RchiOBHm_Chr5g0021001

Zinc transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002306
Physical Location & Seq
Forward (+)
15258071 .. 15259976
1906 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30107

Sequence Viewer

Length: 1065 bp
ATGAGAAAGCTTACTCTATCTTCCACTCTTCTTCTTCTTTTGCTGTTCGTCCCCATCTTGGTTTCTGCAGAATGTACCCGCAGTGATGAATCCCAAGGCCGCGACAAGGGGGAGGCTCTCAAGTTCAAGCTAGTTGCAATAAGTTCAATCCTCATAGCCAGTGCTATTGGTGTCTGTCTCCCAATGTTGGGCAAGAAAATCCCAACTTTGCGTCCTGAAAATGATATTTTCTTCATGATCAAAGCCTTTGCAGCCGGTGTGATACTAGCCACCGGGTTCATCCATGTCTTGCCGGATGCATTTGAAAGCTTAACAAGCCCCTGCCTCAGCCAAAGGCCATGGGGGGATTTCCCGTTCACCGGCTTCATTGCAATGCTTTCGGCTATAGGGACGATGATGATCGACACCTTTGCTACTAGCTATTATAGAAGATCACATTTCAATAAAGCACTGCCAGTGAAAGATGACCAAGAAATGAATGGCGTGCATGATGGTCATGTTCATGTTCATACTCATGCCACACATGGCCACGCCCATGGATCGGACGCGATAGTCTTGGAGGATTCATCGTCTTCTTTCGAGCTCGTCAGGCGACGAATAATATCACAGGTCCTGGAGCTTGGGATTGTGGTTCATTCTGTAATCATTGGAGTATCTTTAGGTGCCTCTCAGAGTCCCAAGACAATCAAGCCTCTAGTTGCAGCGCTGACATTCCATCAATTTTTTGAAGGCATGGGCCTTGGCGGTTGCATCTGCCAGGCTAACTTCAAGTCGAAGGCCATTGCAACTATGGTACTTTTCTTCTCCCTCACCACCCCAATTGGAATAGCTGTTGGAATGGGGGTGTCAAGTTTTTACAAGGAGAATAGCAGTGCTGCTCTGATTGTGGAAGGAGCATTCAACTCTGCTTCAGCTGGGATCCTAATCTACATGGCTCTGGTTGACCTTCTAGCAGCTGACTTTATGAATCCTAGAATGCAGAGTAACCTTAGGATCCAGCTTGGGGCTAATCTTTCACTTGTTTTAGGGTCAGGATGTATGTCTCTCTTGGCCAAATGGGCATGA

Protein Analysis

354

Amino Acids

38.02

Weight (kDa)

8.21

Isoelectric Point (pI)

36.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zip PF02535 41 - 351 6.6e-83 ZIP Zinc transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 551
AccB1I GGYRCC 1 cut(s) 662
AccII CGCG 2 cut(s) 102, 548
AciI CCGC 3 cut(s) 79, 100, 744
AclWI GGATC 5 cut(s) 547, 913, 926, 988, 1001
AcoI YGGCCR 2 cut(s) 526, 1050
AcuI CTGAAG 1 cut(s) 894
AfaI GTAC 2 cut(s) 76, 795
AfeI AGCGCT 1 cut(s) 705
AfiI CCNNNNNNNGG 7 cut(s) 58, 106, 187, 188, 359, 541, 1003
AgsI TTSAA 7 cut(s) 127, 147, 305, 442, 728, 769, 901
AjnI CCWGG 2 cut(s) 612, 756
AloI GAACNNNNNNTCC 2 cut(s) 338, 370
Alw21I GWGCWC 1 cut(s) 585
Alw26I GTCTC 2 cut(s) 182, 1047
AlwI GGATC 5 cut(s) 547, 913, 926, 988, 1001
AlwNI CAGNNNCTG 1 cut(s) 613
Aor51HI AGCGCT 1 cut(s) 705
AoxI GGCC 6 cut(s) 97, 335, 526, 736, 777, 1050
ApeKI GCWGC 4 cut(s) 251, 701, 875, 953
AspLEI GCGC 1 cut(s) 706
AspS9I GGNCC 2 cut(s) 610, 736
AsuC2I CCSGG 1 cut(s) 274
AsuHPI GGTGA 2 cut(s) 349, 802
AvaII GGWCC 1 cut(s) 610
AxyI CCTNAGG 1 cut(s) 989
BalI TGGCCA 2 cut(s) 528, 1052
BamHI GGATCC 2 cut(s) 918, 993
BanI GGYRCC 1 cut(s) 662
BanII GRGCYC 1 cut(s) 585
BbsI GAAGAC 1 cut(s) 564
Bbv12I GWGCWC 1 cut(s) 585
BbvCI CCTCAGC 1 cut(s) 326
BbvI GCAGC 4 cut(s) 263, 713, 862, 965
BccI CCATC 3 cut(s) 62, 485, 723
BcgI CGANNNNNNTGC 4 cut(s) 360, 392, 394, 426
BciT130I CCWGG 2 cut(s) 614, 758
BclI TGATCA 1 cut(s) 237
BcnI CCSGG 1 cut(s) 274
BcoDI GTCTC 2 cut(s) 182, 1047
BfaI CTAG 6 cut(s) 131, 266, 417, 695, 950, 972
BfmI CTRYAG 2 cut(s) 66, 384
BfoI RGCGCY 1 cut(s) 707
BglI GCCNNNNNGGC 1 cut(s) 1058
BisI GCNGC 5 cut(s) 100, 252, 702, 876, 954
BlsI GCNGC 5 cut(s) 101, 253, 703, 877, 955
Bme1390I CCNGG 3 cut(s) 274, 614, 758
Bme18I GGWCC 1 cut(s) 610
BmgT120I GGNCC 2 cut(s) 610, 736
BmiI GGNNCC 3 cut(s) 664, 920, 995
BmrFI CCNGG 3 cut(s) 274, 614, 758
BmsI GCATC 2 cut(s) 286, 759
BpiI GAAGAC 1 cut(s) 564
BpmI CTGGAG 1 cut(s) 635
Bpu10I CCTNAGC 1 cut(s) 326
BpuEI CTTGAG 1 cut(s) 104
BpuMI CCSGG 1 cut(s) 274
BsaBI GATNNNNATC 2 cut(s) 398, 923
BsaJI CCNNGG 4 cut(s) 94, 338, 535, 739
Bsc4I CCNNNNNNNGG 7 cut(s) 58, 106, 187, 188, 359, 541, 1003
Bse118I RCCGGY 2 cut(s) 254, 359
Bse1I ACTGG 2 cut(s) 159, 455
Bse21I CCTNAGG 1 cut(s) 989
Bse3DI GCAATG 3 cut(s) 366, 378, 780
Bse8I GATNNNNATC 2 cut(s) 398, 923
BseBI CCWGG 2 cut(s) 614, 758
BseDI CCNNGG 4 cut(s) 94, 338, 535, 739
BseGI GGATG 3 cut(s) 279, 301, 1040
BseJI GATNNNNATC 2 cut(s) 398, 923
BseLI CCNNNNNNNGG 7 cut(s) 58, 106, 187, 188, 359, 541, 1003
BseMI GCAATG 3 cut(s) 366, 378, 780
BseMII CTCAG 2 cut(s) 340, 683
BseNI ACTGG 2 cut(s) 159, 455
BseXI GCAGC 4 cut(s) 263, 713, 862, 965
BseYI CCCAGC 1 cut(s) 914
Bsh1236I CGCG 2 cut(s) 102, 548
BshFI GGCC 6 cut(s) 99, 337, 528, 738, 779, 1052
BshNI GGYRCC 1 cut(s) 662
BsiHKAI GWGCWC 1 cut(s) 585
BsiSI CCGG 4 cut(s) 255, 273, 293, 360
BslFI GGGAC 3 cut(s) 35, 403, 660
BslI CCNNNNNNNGG 7 cut(s) 58, 106, 187, 188, 359, 541, 1003
BsmAI GTCTC 2 cut(s) 182, 1047
BsmFI GGGAC 3 cut(s) 35, 403, 660
BsmI GAATGC 2 cut(s) 896, 981
BsnI GGCC 6 cut(s) 99, 337, 528, 738, 779, 1052
Bsp1286I GDGCHC 1 cut(s) 585
Bsp143I GATC 6 cut(s) 237, 399, 431, 539, 918, 993
Bsp19I CCATGG 2 cut(s) 338, 535
BspACI CCGC 3 cut(s) 79, 100, 744
BspANI GGCC 6 cut(s) 99, 337, 528, 738, 779, 1052
BspCNI CTCAG 2 cut(s) 339, 682
BspFNI CGCG 2 cut(s) 102, 548
BspHI TCATGA 1 cut(s) 234
BspLI GGNNCC 3 cut(s) 664, 920, 995
BspMAI CTGCAG 1 cut(s) 70
BspPI GGATC 5 cut(s) 547, 913, 926, 988, 1001
BspT107I GGYRCC 1 cut(s) 662
BsrDI GCAATG 3 cut(s) 366, 378, 780
BsrFI RCCGGY 2 cut(s) 254, 359
BsrI ACTGG 2 cut(s) 159, 455
BssAI RCCGGY 2 cut(s) 254, 359
BssECI CCNNGG 4 cut(s) 94, 338, 535, 739
BssMI GATC 6 cut(s) 237, 399, 431, 539, 918, 993
BssT1I CCWWGG 4 cut(s) 94, 338, 535, 739
Bst2UI CCWGG 2 cut(s) 614, 758
Bst6I CTCTTC 1 cut(s) 33
BstC8I GCNNGC 1 cut(s) 485
BstDEI CTNAG 3 cut(s) 326, 669, 989
BstDSI CCRYGG 2 cut(s) 338, 535
BstF5I GGATG 3 cut(s) 279, 301, 1040
BstFNI CGCG 2 cut(s) 102, 548
BstH2I RGCGCY 1 cut(s) 707
BstHHI GCGC 1 cut(s) 706
BstKTI GATC 6 cut(s) 240, 402, 434, 542, 921, 996
BstMAI GTCTC 2 cut(s) 182, 1047
BstMBI GATC 6 cut(s) 237, 399, 431, 539, 918, 993
BstMWI GCNNNNNNNGC 4 cut(s) 251, 315, 589, 1058
BstNI CCWGG 2 cut(s) 614, 758
BstSCI CCNGG 3 cut(s) 272, 612, 756
BstSFI CTRYAG 2 cut(s) 66, 384
BstUI CGCG 2 cut(s) 102, 548
BstV1I GCAGC 4 cut(s) 263, 713, 862, 965
BstV2I GAAGAC 1 cut(s) 564
BstX2I RGATCY 2 cut(s) 918, 993
BstXI CCANNNNNNTGG 1 cut(s) 536
BstYI RGATCY 2 cut(s) 918, 993
Bsu36I CCTNAGG 1 cut(s) 989
BsuRI GGCC 6 cut(s) 99, 337, 528, 738, 779, 1052
BtgI CCRYGG 2 cut(s) 338, 535
BtsCI GGATG 3 cut(s) 279, 301, 1040
BtsI GCAGTG 3 cut(s) 88, 449, 877
BtsIMutI CAGTG 5 cut(s) 88, 166, 449, 462, 877
Cac8I GCNNGC 1 cut(s) 485
CaiI CAGNNNCTG 1 cut(s) 613
CciI TCATGA 1 cut(s) 234
CfoI GCGC 1 cut(s) 706
Cfr10I RCCGGY 2 cut(s) 254, 359
Cfr13I GGNCC 2 cut(s) 610, 736
CseI GACGC 2 cut(s) 200, 554
Csp6I GTAC 2 cut(s) 75, 794
CspCI CAANNNNNGTGG 2 cut(s) 802, 837
CviQI GTAC 2 cut(s) 75, 794
DdeI CTNAG 3 cut(s) 326, 669, 989
DpnI GATC 6 cut(s) 239, 401, 433, 541, 920, 995
DpnII GATC 6 cut(s) 237, 399, 431, 539, 918, 993
DrdI GACNNNNNNGTC 1 cut(s) 551
DseDI GACNNNNNNGTC 1 cut(s) 551
EaeI YGGCCR 2 cut(s) 526, 1050
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
Ecl136II GAGCTC 1 cut(s) 583
Eco130I CCWWGG 4 cut(s) 94, 338, 535, 739
Eco24I GRGCYC 1 cut(s) 585
Eco47I GGWCC 1 cut(s) 610
Eco47III AGCGCT 1 cut(s) 705
Eco53kI GAGCTC 1 cut(s) 583
Eco57I CTGAAG 1 cut(s) 894
Eco81I CCTNAGG 1 cut(s) 989
EcoICRI GAGCTC 1 cut(s) 583
EcoO109I RGGNCCY 1 cut(s) 610
EcoRII CCWGG 2 cut(s) 612, 756
EcoT14I CCWWGG 4 cut(s) 94, 338, 535, 739
EcoT22I ATGCAT 1 cut(s) 301
EcoT38I GRGCYC 1 cut(s) 585
ErhI CCWWGG 4 cut(s) 94, 338, 535, 739
FaqI GGGAC 3 cut(s) 35, 403, 660
FauI CCCGC 1 cut(s) 86
FbaI TGATCA 1 cut(s) 237
Fnu4HI GCNGC 5 cut(s) 100, 252, 702, 876, 954
FokI GGATG 3 cut(s) 266, 308, 1047
FriOI GRGCYC 1 cut(s) 585
Fsp4HI GCNGC 5 cut(s) 100, 252, 702, 876, 954
FspBI CTAG 6 cut(s) 131, 266, 417, 695, 950, 972
GlaI GCGC 1 cut(s) 705
GluI GCNGC 5 cut(s) 100, 252, 702, 876, 954
GsaI CCCAGC 1 cut(s) 918
GsuI CTGGAG 1 cut(s) 635
HaeII RGCGCY 1 cut(s) 707
HaeIII GGCC 6 cut(s) 99, 337, 528, 738, 779, 1052
HapII CCGG 4 cut(s) 255, 273, 293, 360
HgaI GACGC 2 cut(s) 200, 554
HhaI GCGC 1 cut(s) 706
Hin6I GCGC 1 cut(s) 704
HinP1I GCGC 1 cut(s) 704
HincII GTYRAC 1 cut(s) 943
HindII GTYRAC 1 cut(s) 943
HindIII AAGCTT 2 cut(s) 8, 307
HinfI GANTC 4 cut(s) 89, 563, 673, 967
HpaII CCGG 4 cut(s) 255, 273, 293, 360
HphI GGTGA 2 cut(s) 349, 802
Hpy166II GTNNAC 2 cut(s) 357, 943
Hpy188I TCNGA 3 cut(s) 544, 672, 882
Hpy188III TCNNGA 3 cut(s) 215, 235, 1032
Hpy8I GTNNAC 2 cut(s) 357, 943
Hpy99I CGWCG 1 cut(s) 597
HpyAV CCTTC 4 cut(s) 722, 769, 884, 956
HpyF10VI GCNNNNNNNGC 4 cut(s) 251, 315, 589, 1058
HpyF3I CTNAG 3 cut(s) 326, 669, 989
HspAI GCGC 1 cut(s) 704
Ksp22I TGATCA 1 cut(s) 237
Kzo9I GATC 6 cut(s) 237, 399, 431, 539, 918, 993
LmnI GCTCC 2 cut(s) 616, 893
Lsp1109I GCAGC 4 cut(s) 263, 713, 862, 965
LweI GCATC 2 cut(s) 286, 759
MaeI CTAG 6 cut(s) 131, 266, 417, 695, 950, 972
MaeIII GTNAC 1 cut(s) 983
MalI GATC 6 cut(s) 239, 401, 433, 541, 920, 995
MboI GATC 6 cut(s) 237, 399, 431, 539, 918, 993
MboII GAAGA 8 cut(s) 12, 20, 23, 26, 223, 441, 564, 793
MfeI CAATTG 1 cut(s) 819
MflI RGATCY 2 cut(s) 918, 993
MhlI GDGCHC 1 cut(s) 585
MlsI TGGCCA 2 cut(s) 528, 1052
MluCI AATT 2 cut(s) 719, 819
MluNI TGGCCA 2 cut(s) 528, 1052
MlyI GAGTC 1 cut(s) 682
MmeI TCCRAC 1 cut(s) 814
MnlI CCTC 7 cut(s) 106, 161, 335, 553, 676, 702, 818
Mox20I TGGCCA 2 cut(s) 528, 1052
Mph1103I ATGCAT 1 cut(s) 301
MscI TGGCCA 2 cut(s) 528, 1052
MseI TTAA 1 cut(s) 311
MslI CAYNNNNRTG 4 cut(s) 371, 501, 513, 534
Msp20I TGGCCA 2 cut(s) 528, 1052
MspA1I CMGCKG 2 cut(s) 914, 956
MspI CCGG 4 cut(s) 255, 273, 293, 360
MspR9I CCNGG 3 cut(s) 274, 614, 758
MunI CAATTG 1 cut(s) 819
Mva1269I GAATGC 2 cut(s) 896, 981
MvaI CCWGG 2 cut(s) 614, 758
MvnI CGCG 2 cut(s) 102, 548
MwoI GCNNNNNNNGC 4 cut(s) 251, 315, 589, 1058
NciI CCSGG 1 cut(s) 274
NcoI CCATGG 2 cut(s) 338, 535
NdeII GATC 6 cut(s) 237, 399, 431, 539, 918, 993
NlaIV GGNNCC 3 cut(s) 664, 920, 995
NsiI ATGCAT 1 cut(s) 301
PagI TCATGA 1 cut(s) 234
PctI GAATGC 2 cut(s) 896, 981
PfeI GAWTC 3 cut(s) 89, 563, 967
PfoI TCCNGGA 1 cut(s) 612
PkrI GCNGC 5 cut(s) 101, 253, 703, 877, 955
PleI GAGTC 1 cut(s) 681
PpsI GAGTC 1 cut(s) 681
PpuMI RGGWCCY 1 cut(s) 610
Psp124BI GAGCTC 1 cut(s) 585
Psp5II RGGWCCY 1 cut(s) 610
Psp6I CCWGG 2 cut(s) 612, 756
PspFI CCCAGC 1 cut(s) 914
PspGI CCWGG 2 cut(s) 612, 756
PspN4I GGNNCC 3 cut(s) 664, 920, 995
PspPI GGNCC 2 cut(s) 610, 736
PspPPI RGGWCCY 1 cut(s) 610
PstI CTGCAG 1 cut(s) 70
PstNI CAGNNNCTG 1 cut(s) 613
PsuI RGATCY 2 cut(s) 918, 993
PvuII CAGCTG 2 cut(s) 914, 956
RsaI GTAC 2 cut(s) 76, 795
RsaNI GTAC 2 cut(s) 75, 794
RseI CAYNNNNRTG 4 cut(s) 371, 501, 513, 534
SacI GAGCTC 1 cut(s) 585
SaqAI TTAA 1 cut(s) 311
SatI GCNGC 5 cut(s) 100, 252, 702, 876, 954
Sau3AI GATC 6 cut(s) 237, 399, 431, 539, 918, 993
Sau96I GGNCC 2 cut(s) 610, 736
SchI GAGTC 1 cut(s) 682
ScrFI CCNGG 3 cut(s) 274, 614, 758
SduI GDGCHC 1 cut(s) 585
SfaNI GCATC 2 cut(s) 286, 759
SfcI CTRYAG 2 cut(s) 66, 384
SinI GGWCC 1 cut(s) 610
SmiMI CAYNNNNRTG 4 cut(s) 371, 501, 513, 534
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
Sse9I AATT 2 cut(s) 719, 819
SsiI CCGC 3 cut(s) 79, 100, 744
SspMI CTAG 6 cut(s) 131, 266, 417, 695, 950, 972
SstI GAGCTC 1 cut(s) 585
StyD4I CCNGG 3 cut(s) 272, 612, 756
StyI CCWWGG 4 cut(s) 94, 338, 535, 739
TaqI TCGA 3 cut(s) 402, 579, 773
TasI AATT 2 cut(s) 719, 819
TauI GCSGC 1 cut(s) 102
TfiI GAWTC 3 cut(s) 89, 563, 967
Tru1I TTAA 1 cut(s) 311
Tru9I TTAA 1 cut(s) 311
TscAI CASTG 5 cut(s) 88, 166, 456, 462, 877
TseI GCWGC 4 cut(s) 251, 701, 875, 953
TspRI CASTG 5 cut(s) 88, 166, 456, 462, 877
VpaK11BI GGWCC 1 cut(s) 610
XcmI CCANNNNNNNNNTGG 2 cut(s) 476, 787
XspI CTAG 6 cut(s) 131, 266, 417, 695, 950, 972
Zsp2I ATGCAT 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.