AT4G05110

Transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001163
Physical Location & Seq
Reverse (-)
2624867 .. 2629058
4192 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G05110.2

Sequence Viewer

Length: 1257 bp
ATGGCGGATATATACGAGCACCAAGTTCCTGAGAAGCTTCGCGGGAAGTATCAAGCAATGATTGTCTACTGTATTCTTGGATTTGGTTCTCTTATTTCTTGGAACAGTATGCTCACTACAGCAGATTACTACTACAAAGTTTTCCCGGATTATCATCCTTCAAGAGTACTCACACTTGTATACCAACCGTTTGCGTTTGGAGCAATCGTGATCCTCGCATACCACGAATCGAAAACCAGTACTCGAAAACGTAACCTGATTGGTTACATTCTATACACAATATCCACATTCTTGCTCATAGTTTTGGATTTGGCAACAAAAGGACGTGGTGGATTCGGACCTTATACCGGTTTATGCGCAGTCGTTGCTGCTTTTGGCCTCGCGGATGCTACCGTTCAAGGAGGAATGTTCGGTGATTTATCACTGATGTGCCCTGAACTAGTTCAGTCATACATGGGAGGTATGGCTGTAGCTGGTGCGCTAACATCAGCGCTTAGGCTAATAACCAAAGCAGCTTTTGAGAAATCAAACAATGGTCTACGAAAAGGGGCAATGATGTTCTTAGCTATCTCCACATGCATAGAGTTACTATCTGTGATGCTATATGCTTATGTGTTACCGAAACTCCCGATTGTTATGTATTACCGAAGAAAAGCCGCTTCTCAAGGATCCAAAACTGTTTCTGCTGATCTTGCTGCTGCGGGTATCCAAAATCAATCAGACTTGAGTGATGATGATTCCAAGAATCAAAGGCTAAGCAAAAAAGAGCTGTTGTTTCAAAACATAGACCATGCAGTAAATTTATTCCTCATCTACGTTTGTACATTATCCATTTTTCCCGGGTTCTTGTATGAAAACACAGGACAACACGGGCTAGGTGCTTGGTACGCACTTGTCTTAGTAGCGATGTACAATTGTTGGGATTTGGTCGGGAGGTACACACCACTGGTTAAATGGCTCAATATTGAGAATAGAAAATTGATCACAATTGCGGTTTTGTCACGTTATCTCCTCATTCCGGCGTTCTACTTCACTGCGAAATATGGTGATCAAGGATGGATGATTATGCTCGTTTCTGTTTTGGGATTGACTAATGGACATCTCACTGTTTGCATTATGACTATAGCTCCTAAAGGATACAAGGGTCCGGAGCAGAATGCGTTAGGGAACTTGCTGGTGATATTTCTGTTAGGAGGGATATTTGCAGGAGTTGCTTTGGATTGGTTATGGCTTATTGGTAAGAAGAATGCCTTTTGA

Protein Analysis

418

Amino Acids

46.22

Weight (kDa)

9.08

Isoelectric Point (pI)

30.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleoside_tran PF01733 121 - 410 2e-31 Nucleoside transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 358
AccI GTMKAC 3 cut(s) 66, 180, 538
AccII CGCG 2 cut(s) 42, 383
AccIII TCCGGA 1 cut(s) 1147
AciI CCGC 6 cut(s) 5, 42, 383, 657, 701, 992
AclWI GGATC 3 cut(s) 205, 663, 676
AcsI RAATTY 1 cut(s) 799
AfaI GTAC 6 cut(s) 168, 241, 823, 887, 911, 938
AfeI AGCGCT 1 cut(s) 492
AfiI CCNNNNNNNGG 2 cut(s) 347, 1018
AgeI ACCGGT 1 cut(s) 347
AgsI TTSAA 3 cut(s) 162, 398, 779
AhlI ACTAGT 1 cut(s) 439
AjiI CACGTC 1 cut(s) 326
AleI CACNNNNGTG 1 cut(s) 427
AluBI AGCT 6 cut(s) 37, 473, 515, 566, 769, 1127
AluI AGCT 6 cut(s) 37, 473, 515, 566, 769, 1127
Alw21I GWGCWC 1 cut(s) 21
AlwI GGATC 3 cut(s) 205, 663, 676
Ama87I CYCGRG 1 cut(s) 839
Aor13HI TCCGGA 1 cut(s) 1147
Aor51HI AGCGCT 1 cut(s) 492
AoxI GGCC 1 cut(s) 376
ApeKI GCWGC 4 cut(s) 368, 512, 695, 698
ApoI RAATTY 1 cut(s) 799
AsiGI ACCGGT 1 cut(s) 347
Asp700I GAANNNNTTC 1 cut(s) 441
AspLEI GCGC 3 cut(s) 359, 481, 493
AspS9I GGNCC 2 cut(s) 338, 1145
AsuC2I CCSGG 3 cut(s) 146, 840, 841
AsuHPI GGTGA 3 cut(s) 425, 1058, 1189
AvaI CYCGRG 1 cut(s) 839
AvaII GGWCC 2 cut(s) 338, 1145
BaeGI GKGCMC 1 cut(s) 434
BaeI ACNNNNGTAYC 2 cut(s) 1129, 1162
BamHI GGATCC 1 cut(s) 668
Bbv12I GWGCWC 1 cut(s) 21
BbvI GCAGC 4 cut(s) 355, 524, 682, 685
BccI CCATC 1 cut(s) 1050
BciVI GTATCC 2 cut(s) 716, 1130
BclI TGATCA 2 cut(s) 981, 1048
BcnI CCSGG 3 cut(s) 146, 840, 841
BcuI ACTAGT 1 cut(s) 439
BfaI CTAG 2 cut(s) 440, 875
BfmI CTRYAG 3 cut(s) 117, 468, 1122
BfoI RGCGCY 1 cut(s) 494
BfuI GTATCC 2 cut(s) 716, 1130
BisI GCNGC 5 cut(s) 369, 513, 657, 696, 699
BlpI GCTNAGC 1 cut(s) 755
BlsI GCNGC 5 cut(s) 370, 514, 658, 697, 700
BmcAI AGTACT 2 cut(s) 168, 241
Bme1390I CCNGG 3 cut(s) 146, 840, 841
Bme18I GGWCC 2 cut(s) 338, 1145
BmeT110I CYCGRG 1 cut(s) 839
BmgBI CACGTC 1 cut(s) 326
BmgT120I GGNCC 2 cut(s) 338, 1145
BmiI GGNNCC 2 cut(s) 670, 1146
BmrFI CCNGG 3 cut(s) 146, 840, 841
BmsI GCATC 2 cut(s) 376, 588
Bpu10I CCTNAGC 1 cut(s) 494
Bpu1102I GCTNAGC 1 cut(s) 755
BpuEI CTTGAG 2 cut(s) 648, 745
BpuMI CCSGG 3 cut(s) 146, 840, 841
BsaBI GATNNNNATC 1 cut(s) 153
BsaJI CCNNGG 1 cut(s) 839
BsaWI WCCGGW 2 cut(s) 347, 1147
BsaXI ACNNNNNCTCC 8 cut(s) 192, 222, 609, 639, 993, 1023, 1111, 1141
Bsc4I CCNNNNNNNGG 2 cut(s) 347, 1018
Bse118I RCCGGY 1 cut(s) 347
Bse1I ACTGG 2 cut(s) 237, 951
Bse3DI GCAATG 2 cut(s) 63, 558
Bse8I GATNNNNATC 1 cut(s) 153
BseAI TCCGGA 1 cut(s) 1147
BseDI CCNNGG 1 cut(s) 839
BseGI GGATG 4 cut(s) 154, 391, 1061, 1065
BseJI GATNNNNATC 1 cut(s) 153
BseLI CCNNNNNNNGG 2 cut(s) 347, 1018
BseMI GCAATG 2 cut(s) 63, 558
BseMII CTCAG 1 cut(s) 21
BseNI ACTGG 2 cut(s) 237, 951
BseRI GAGGAG 1 cut(s) 1001
BseSI GKGCMC 1 cut(s) 434
BseXI GCAGC 4 cut(s) 355, 524, 682, 685
Bsh1236I CGCG 2 cut(s) 42, 383
BshFI GGCC 1 cut(s) 378
BshTI ACCGGT 1 cut(s) 347
BsiHKAI GWGCWC 1 cut(s) 21
BsiHKCI CYCGRG 1 cut(s) 839
BsiSI CCGG 5 cut(s) 146, 348, 840, 1019, 1148
BslI CCNNNNNNNGG 2 cut(s) 347, 1018
BsmI GAATGC 2 cut(s) 1162, 1252
BsnI GGCC 1 cut(s) 378
BsoBI CYCGRG 1 cut(s) 839
Bsp1286I GDGCHC 2 cut(s) 21, 434
Bsp13I TCCGGA 1 cut(s) 1147
Bsp1407I TGTACA 2 cut(s) 821, 909
Bsp143I GATC 5 cut(s) 210, 668, 688, 981, 1048
Bsp1720I GCTNAGC 1 cut(s) 755
BspACI CCGC 6 cut(s) 5, 42, 383, 657, 701, 992
BspANI GGCC 1 cut(s) 378
BspCNI CTCAG 1 cut(s) 22
BspEI TCCGGA 1 cut(s) 1147
BspFNI CGCG 2 cut(s) 42, 383
BspLI GGNNCC 2 cut(s) 670, 1146
BspPI GGATC 3 cut(s) 205, 663, 676
BsrDI GCAATG 2 cut(s) 63, 558
BsrFI RCCGGY 1 cut(s) 347
BsrGI TGTACA 2 cut(s) 821, 909
BsrI ACTGG 2 cut(s) 237, 951
BssAI RCCGGY 1 cut(s) 347
BssECI CCNNGG 1 cut(s) 839
BssMI GATC 5 cut(s) 210, 668, 688, 981, 1048
BssNAI GTATAC 1 cut(s) 181
Bst1107I GTATAC 1 cut(s) 181
Bst4CI ACNGT 6 cut(s) 71, 107, 189, 394, 679, 1108
BstAPI GCANNNNNTGC 2 cut(s) 365, 1211
BstAUI TGTACA 2 cut(s) 821, 909
BstDEI CTNAG 5 cut(s) 30, 494, 562, 755, 898
BstF5I GGATG 4 cut(s) 154, 391, 1061, 1065
BstFNI CGCG 2 cut(s) 42, 383
BstH2I RGCGCY 1 cut(s) 494
BstHHI GCGC 3 cut(s) 359, 481, 493
BstKTI GATC 5 cut(s) 213, 671, 691, 984, 1051
BstMBI GATC 5 cut(s) 210, 668, 688, 981, 1048
BstMWI GCNNNNNNNGC 5 cut(s) 200, 365, 692, 887, 1211
BstNSI RCATGY 1 cut(s) 579
BstSCI CCNGG 3 cut(s) 144, 838, 839
BstSFI CTRYAG 3 cut(s) 117, 468, 1122
BstSLI GKGCMC 1 cut(s) 434
BstUI CGCG 2 cut(s) 42, 383
BstV1I GCAGC 4 cut(s) 355, 524, 682, 685
BstX2I RGATCY 1 cut(s) 668
BstYI RGATCY 1 cut(s) 668
BstZ17I GTATAC 1 cut(s) 181
BsuI GTATCC 2 cut(s) 716, 1130
BsuRI GGCC 1 cut(s) 378
BtgZI GCGATG 1 cut(s) 920
BtrI CACGTC 1 cut(s) 326
BtsCI GGATG 4 cut(s) 154, 391, 1061, 1065
BtsI GCAGTG 1 cut(s) 1032
BtsIMutI CAGTG 4 cut(s) 422, 944, 1032, 1104
CfoI GCGC 3 cut(s) 359, 481, 493
Cfr10I RCCGGY 1 cut(s) 347
Cfr13I GGNCC 2 cut(s) 338, 1145
Cfr9I CCCGGG 1 cut(s) 839
Csp6I GTAC 6 cut(s) 167, 240, 822, 886, 910, 937
CspAI ACCGGT 1 cut(s) 347
CviAII CATG 3 cut(s) 454, 576, 791
CviQI GTAC 6 cut(s) 167, 240, 822, 886, 910, 937
DdeI CTNAG 5 cut(s) 30, 494, 562, 755, 898
DpnI GATC 5 cut(s) 212, 670, 690, 983, 1050
DpnII GATC 5 cut(s) 210, 668, 688, 981, 1048
EciI GGCGGA 1 cut(s) 20
Eco47I GGWCC 2 cut(s) 338, 1145
Eco47III AGCGCT 1 cut(s) 492
Eco88I CYCGRG 1 cut(s) 839
EcoT22I ATGCAT 1 cut(s) 581
FaeI CATG 3 cut(s) 457, 579, 794
FalI AAGNNNNNCTT 1 cut(s) 1235
FatI CATG 3 cut(s) 453, 575, 790
FauI CCCGC 2 cut(s) 35, 694
FbaI TGATCA 2 cut(s) 981, 1048
FblI GTMKAC 3 cut(s) 66, 180, 538
Fnu4HI GCNGC 5 cut(s) 369, 513, 657, 696, 699
FokI GGATG 4 cut(s) 141, 398, 1068, 1072
Fsp4HI GCNGC 5 cut(s) 369, 513, 657, 696, 699
FspBI CTAG 2 cut(s) 440, 875
FspI TGCGCA 1 cut(s) 358
GlaI GCGC 3 cut(s) 358, 480, 492
GluI GCNGC 5 cut(s) 369, 513, 657, 696, 699
HaeII RGCGCY 1 cut(s) 494
HaeIII GGCC 1 cut(s) 378
HapII CCGG 5 cut(s) 146, 348, 840, 1019, 1148
HhaI GCGC 3 cut(s) 359, 481, 493
Hin1II CATG 3 cut(s) 457, 579, 794
Hin6I GCGC 3 cut(s) 357, 479, 491
HinP1I GCGC 3 cut(s) 357, 479, 491
HindIII AAGCTT 1 cut(s) 35
HinfI GANTC 4 cut(s) 227, 333, 737, 745
HpaII CCGG 5 cut(s) 146, 348, 840, 1019, 1148
HphI GGTGA 3 cut(s) 425, 1058, 1189
Hpy166II GTNNAC 4 cut(s) 67, 181, 539, 939
Hpy188I TCNGA 2 cut(s) 338, 721
Hpy188III TCNNGA 6 cut(s) 29, 162, 208, 628, 931, 1148
Hpy8I GTNNAC 4 cut(s) 67, 181, 539, 939
HpyAV CCTTC 1 cut(s) 168
HpyCH4III ACNGT 6 cut(s) 71, 107, 189, 394, 679, 1108
HpyCH4IV ACGT 4 cut(s) 250, 325, 816, 1003
HpyCH4V TGCA 4 cut(s) 579, 794, 1113, 1205
HpyF10VI GCNNNNNNNGC 5 cut(s) 200, 365, 692, 887, 1211
HpyF3I CTNAG 5 cut(s) 30, 494, 562, 755, 898
HpySE526I ACGT 4 cut(s) 250, 325, 816, 1003
Hsp92II CATG 3 cut(s) 457, 579, 794
HspAI GCGC 3 cut(s) 357, 479, 491
Kpn2I TCCGGA 1 cut(s) 1147
Ksp22I TGATCA 2 cut(s) 981, 1048
Kzo9I GATC 5 cut(s) 210, 668, 688, 981, 1048
LmnI GCTCC 3 cut(s) 200, 1132, 1150
Lsp1109I GCAGC 4 cut(s) 355, 524, 682, 685
LweI GCATC 2 cut(s) 376, 588
MaeI CTAG 2 cut(s) 440, 875
MaeII ACGT 4 cut(s) 250, 325, 816, 1003
MaeIII GTNAC 5 cut(s) 251, 263, 585, 615, 999
MalI GATC 5 cut(s) 212, 670, 690, 983, 1050
MboI GATC 5 cut(s) 210, 668, 688, 981, 1048
MboII GAAGA 2 cut(s) 660, 1255
MfeI CAATTG 2 cut(s) 913, 987
MflI RGATCY 1 cut(s) 668
MhlI GDGCHC 2 cut(s) 21, 434
MluCI AATT 4 cut(s) 799, 913, 977, 987
MnlI CCTC 8 cut(s) 224, 389, 395, 452, 818, 927, 1022, 1187
Mph1103I ATGCAT 1 cut(s) 581
MroI TCCGGA 1 cut(s) 1147
MroXI GAANNNNTTC 1 cut(s) 441
MseI TTAA 1 cut(s) 951
MslI CAYNNNNRTG 1 cut(s) 427
MspI CCGG 5 cut(s) 146, 348, 840, 1019, 1148
MspR9I CCNGG 3 cut(s) 146, 840, 841
MunI CAATTG 2 cut(s) 913, 987
Mva1269I GAATGC 2 cut(s) 1162, 1252
MvnI CGCG 2 cut(s) 42, 383
MwoI GCNNNNNNNGC 5 cut(s) 200, 365, 692, 887, 1211
NciI CCSGG 3 cut(s) 146, 840, 841
NdeII GATC 5 cut(s) 210, 668, 688, 981, 1048
NlaIII CATG 3 cut(s) 457, 579, 794
NlaIV GGNNCC 2 cut(s) 670, 1146
NmuCI GTSAC 1 cut(s) 999
NsbI TGCGCA 1 cut(s) 358
NsiI ATGCAT 1 cut(s) 581
NspI RCATGY 1 cut(s) 579
OliI CACNNNNGTG 1 cut(s) 427
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PctI GAATGC 2 cut(s) 1162, 1252
PdmI GAANNNNTTC 1 cut(s) 441
PfeI GAWTC 4 cut(s) 227, 333, 737, 745
PfoI TCCNGGA 1 cut(s) 144
PinAI ACCGGT 1 cut(s) 347
PkrI GCNGC 5 cut(s) 370, 514, 658, 697, 700
PspN4I GGNNCC 2 cut(s) 670, 1146
PspPI GGNCC 2 cut(s) 338, 1145
PsuI RGATCY 1 cut(s) 668
RsaI GTAC 6 cut(s) 168, 241, 823, 887, 911, 938
RsaNI GTAC 6 cut(s) 167, 240, 822, 886, 910, 937
RseI CAYNNNNRTG 1 cut(s) 427
SaqAI TTAA 1 cut(s) 951
SatI GCNGC 5 cut(s) 369, 513, 657, 696, 699
Sau3AI GATC 5 cut(s) 210, 668, 688, 981, 1048
Sau96I GGNCC 2 cut(s) 338, 1145
ScaI AGTACT 2 cut(s) 168, 241
ScrFI CCNGG 3 cut(s) 146, 840, 841
SduI GDGCHC 2 cut(s) 21, 434
SfaNI GCATC 2 cut(s) 376, 588
SfcI CTRYAG 3 cut(s) 117, 468, 1122
SinI GGWCC 2 cut(s) 338, 1145
SmaI CCCGGG 1 cut(s) 841
SmiMI CAYNNNNRTG 1 cut(s) 427
SmlI CTYRAG 2 cut(s) 663, 724
SmoI CTYRAG 2 cut(s) 663, 724
SpeI ACTAGT 1 cut(s) 439
Sse9I AATT 4 cut(s) 799, 913, 977, 987
SsiI CCGC 6 cut(s) 5, 42, 383, 657, 701, 992
SspI AATATT 1 cut(s) 964
SspMI CTAG 2 cut(s) 440, 875
StyD4I CCNGG 3 cut(s) 144, 838, 839
TaaI ACNGT 6 cut(s) 71, 107, 189, 394, 679, 1108
TaiI ACGT 4 cut(s) 253, 328, 819, 1006
TaqI TCGA 2 cut(s) 230, 244
TasI AATT 4 cut(s) 799, 913, 977, 987
TatI WGTACW 4 cut(s) 166, 239, 821, 909
TauI GCSGC 1 cut(s) 659
TfiI GAWTC 4 cut(s) 227, 333, 737, 745
Tru1I TTAA 1 cut(s) 951
Tru9I TTAA 1 cut(s) 951
TscAI CASTG 4 cut(s) 429, 951, 1039, 1111
TseFI GTSAC 1 cut(s) 999
TseI GCWGC 4 cut(s) 368, 512, 695, 698
Tsp45I GTSAC 1 cut(s) 999
TspDTI ATGAA 1 cut(s) 867
TspMI CCCGGG 1 cut(s) 839
TspRI CASTG 4 cut(s) 429, 951, 1039, 1111
VpaK11BI GGWCC 2 cut(s) 338, 1145
XapI RAATTY 1 cut(s) 799
XceI RCATGY 1 cut(s) 579
XcmI CCANNNNNNNNNTGG 1 cut(s) 951
XmaI CCCGGG 1 cut(s) 839
XmiI GTMKAC 3 cut(s) 66, 180, 538
XmnI GAANNNNTTC 1 cut(s) 441
XspI CTAG 2 cut(s) 440, 875
ZrmI AGTACT 2 cut(s) 168, 241
Zsp2I ATGCAT 1 cut(s) 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.