AT4G24220

3-oxo-Delta(4,5)-steroid 5-beta-reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0000626
Physical Location & Seq
Forward (+)
12564945 .. 12566755
1811 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G24220.2

Sequence Viewer

Length: 1167 bp
ATGAGTTGGTGGTGGGCTGGCGCCATCGGAGCTGCCAAGAAGAAACTCGACGAAGATGAACCATCACAAAGCTTCGAGAGCGTCGCTCTCATCATCGGCGTTACTGGAATCGTCGGAAACAGCTTGGCGGAGATTCTCCCTCTTTCCGACACACCCGGTGGTCCATGGAAAGTCTACGGCGTCGCTCGTCGTCCTCGTCCTACCTGGAACGCCGATCATCCGATCGATTACATCCAGTGCGATGTCTCCGACGCCGAAGACACAAGATCCAAGCTTTCCCCTTTAACCGATGTCACACATGTCTTCTACGTCACCTGGACCAATCGTGAATCGGAAAGTGAAAACTGTGAAGCAAATGGCTCAATGCTCCGTAACGTTCTCCAAGCGATTATCCCATACGCGCCAAATCTCCGACATGTTTGTCTCCAGACAGGGACAAAGCACTACCTTGGCCCTTTCACCAACGTTGACGGACCTCGTCACGATCCACCGTTCACTGAGGATATGCCGAGATTGCAGATCCAGAATTTCTATTATACCCAAGAGGATATTCTGTTTGAAGAGATCAAGAAGATAGAAACCGTGACGTGGTCTATCCACAGACCAAACATGATCTTTGGGTTCTCTCCTTATAGTTTGATGAACATTGTTGGGACTCTCTGTGTCTATGCAGCGATATGTAAGCATGAAGGGTCTCCGTTGTTGTTTCCTGGGAGCAAGAAAGCTTGGGAAGGGTTCATGACGGCTTCTGACGCGGATTTGATTGCGGAGCAGCAGATTTGGGCGGCGGTTGATCCGTATGCGAAGAACGAGGCGTTTAACTGCAACAATGCTGATATCTTCAAGTGGAAGCATCTGTGGAAGATTCTAGCTGAGCAGTTTGGGATTGAGGAGTATGGATTTGAGGAAGGGAAGAATTTGGGGTTGGTGGAGATGATGAAAGGGAAAGAGAGAGTGTGGGAGGAGATGGTTAAGGAGAATCAATTGCAAGAGAAGAAGCTTGAGGAAGTTGGTGTGTGGTGGTTTGCTGATGTTATACTTGGAGTTGAAGGAATGATTGATAGTATGAACAAGAGTAAGGAATATGGCTTCCTTGGTTTCAGGAACTCCAACAACTCTTTTATCTCTTGGATTGACAAGTACAAGGCGTTCAAGATCGTACCTTGA

Protein Analysis

388

Amino Acids

44.23

Weight (kDa)

5.09

Isoelectric Point (pI)

41.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRISE PF22917 86 - 387 2.4e-30 PRISE-like Rossmann-fold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000626)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24220 AT4G24220
fragaria_vesca FvH4_2g04580 FvH4_2g04580 FvH4_2g04580 FvH4_4g06071 FvH4_5g25130
malus_domestica MD05G1094500.v1.1 MD05G1108300.v1.1 MD08G1088400.v1.1 MD15G1073200.v1.1 MD15G1073300.v1.1 MD15G1073400.v1.1 MD15G1073500.v1.1 MD15G1073600.v1.1
prunus_persica Prupe.1G426100_v2.0.a1 Prupe.1G426100_v2.0.a1 Prupe.1G426200_v2.0.a1 Prupe.1G426300_v2.0.a1 Prupe.1G426400_v2.0.a1 Prupe.1G426400_v2.0.a1 Prupe.8G139400_v2.0.a1 Prupe.8G139400_v2.0.a1
pyrus_communis pycom05g09350 pycom08g07190 pycom13g22780 pycom15g06840 pycom15g06850 pycom15g06870
rosa_chinensis RchiOBHm_Chr6g0254161 RchiOBHm_Chr6g0254181 RchiOBHm_Chr6g0254191 RchiOBHm_Chr6g0309171 RchiOBHm_Chr6g0309181 RchiOBHm_Chr6g0309191 RchiOBHm_Chr6g0309201
rosa_laevigata RLG00000010582 RLG00000010583 RLG00000014937 RLG00000014938
rosa_multiflora Rmu_co8060540.1_g000001 Rmu_co8332663.1_g000001 Rmu_sc0000010.1_g000017 Rmu_sc0003711.1_g000008 Rmu_sc0016971.1_g000003 Rmu_sc0020102.1_g000006 Rmu_sc0020102.1_g000008
rosa_roxburghii Rroxscaffold_7G00159570 Rroxscaffold_7G00159580 Rroxscaffold_7G00159590 Rroxscaffold_7G00211100
rosa_rugosa Rorug05G0550400 Rorug06G0370100 Rorug06G0370300
rosa_samantha Rh6AG066900 Rh6AG483200 Rh6AG483300 Rh6AG483400 Rh6BG059800 Rh6BG060000 Rh6BG492200 Rh6BG492300 Rh6BG492500 Rh6CG059700 Rh6CG059800 Rh6CG496900 Rh6CG497000 Rh6CG497200 Rh6CG497400 Rh6DG056700 Rh6DG056800 Rh6DG483800 Rh6DG484000
rosa_wichuraiana Rw6G005860 Rw6G005870 Rw6G042070 Rw6G042080 Rw6G042090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 420
AccB1I GGYRCC 1 cut(s) 20
AccI GTMKAC 1 cut(s) 174
AccII CGCG 2 cut(s) 401, 755
AciI CCGC 5 cut(s) 128, 755, 767, 785, 788
AclI AACGTT 2 cut(s) 375, 465
AclWI GGATC 4 cut(s) 261, 479, 514, 788
AcsI RAATTY 2 cut(s) 526, 916
AcyI GRCGYC 3 cut(s) 21, 180, 252
AdeI CACNNNGTG 1 cut(s) 158
AfaI GTAC 2 cut(s) 1142, 1161
AfiI CCNNNNNNNGG 1 cut(s) 588
AflIII ACRYGT 2 cut(s) 298, 415
AgsI TTSAA 4 cut(s) 560, 844, 1049, 1153
AjiI CACGTC 1 cut(s) 588
AjnI CCWGG 3 cut(s) 203, 314, 709
AjuI GAANNNNNNNTTGG 4 cut(s) 534, 566, 908, 940
AluBI AGCT 7 cut(s) 32, 72, 123, 274, 725, 872, 1000
AluI AGCT 7 cut(s) 32, 72, 123, 274, 725, 872, 1000
Alw26I GTCTC 3 cut(s) 250, 428, 699
AlwI GGATC 4 cut(s) 261, 479, 514, 788
AoxI GGCC 1 cut(s) 451
ApeKI GCWGC 3 cut(s) 32, 671, 772
ApoI RAATTY 2 cut(s) 526, 916
AspLEI GCGC 2 cut(s) 23, 403
AspS9I GGNCC 4 cut(s) 161, 318, 452, 473
AsuC2I CCSGG 1 cut(s) 156
AsuHPI GGTGA 2 cut(s) 304, 451
AvaII GGWCC 3 cut(s) 161, 318, 473
BanI GGYRCC 1 cut(s) 20
BbsI GAAGAC 2 cut(s) 264, 295
BbvI GCAGC 3 cut(s) 19, 683, 784
BccI CCATC 3 cut(s) 32, 70, 961
BceAI ACGGC 2 cut(s) 193, 759
BciT130I CCWGG 3 cut(s) 205, 316, 711
BcnI CCSGG 1 cut(s) 156
BcoDI GTCTC 3 cut(s) 250, 428, 699
BfaI CTAG 1 cut(s) 869
BfoI RGCGCY 1 cut(s) 24
BisI GCNGC 4 cut(s) 33, 672, 773, 786
BlpI GCTNAGC 1 cut(s) 873
BlsI GCNGC 4 cut(s) 34, 673, 774, 787
Bme1390I CCNGG 4 cut(s) 156, 205, 316, 711
Bme18I GGWCC 3 cut(s) 161, 318, 473
BmgBI CACGTC 1 cut(s) 588
BmgT120I GGNCC 4 cut(s) 161, 318, 452, 473
BmiI GGNNCC 1 cut(s) 22
BmrFI CCNGG 4 cut(s) 156, 205, 316, 711
BmsI GCATC 1 cut(s) 862
BpiI GAAGAC 2 cut(s) 264, 295
BplI GAGNNNNNCTC 2 cut(s) 70, 102
BpmI CTGGAG 1 cut(s) 410
Bpu1102I GCTNAGC 1 cut(s) 873
BpuEI CTTGAG 1 cut(s) 1022
BpuMI CCSGG 1 cut(s) 156
Bsa29I ATCGAT 1 cut(s) 225
BsaHI GRCGYC 3 cut(s) 21, 180, 252
BsaI GGTCTC 1 cut(s) 699
BsaJI CCNNGG 4 cut(s) 164, 448, 710, 1093
Bsc4I CCNNNNNNNGG 1 cut(s) 588
Bse1I ACTGG 2 cut(s) 109, 235
BseBI CCWGG 3 cut(s) 205, 316, 711
BseCI ATCGAT 1 cut(s) 225
BseDI CCNNGG 4 cut(s) 164, 448, 710, 1093
BseGI GGATG 2 cut(s) 217, 231
BseLI CCNNNNNNNGG 1 cut(s) 588
BseMII CTCAG 2 cut(s) 489, 864
BseNI ACTGG 2 cut(s) 109, 235
BseRI GAGGAG 2 cut(s) 905, 977
BseXI GCAGC 3 cut(s) 19, 683, 784
Bsh1236I CGCG 2 cut(s) 401, 755
Bsh1285I CGRYCG 1 cut(s) 225
BshFI GGCC 1 cut(s) 453
BshNI GGYRCC 1 cut(s) 20
BshVI ATCGAT 1 cut(s) 225
BsiEI CGRYCG 1 cut(s) 225
BsiSI CCGG 1 cut(s) 156
BslFI GGGAC 2 cut(s) 448, 667
BslI CCNNNNNNNGG 1 cut(s) 588
BsmAI GTCTC 3 cut(s) 250, 428, 699
BsmFI GGGAC 2 cut(s) 448, 667
BsnI GGCC 1 cut(s) 453
Bso31I GGTCTC 1 cut(s) 699
Bsp143I GATC 9 cut(s) 214, 222, 266, 484, 519, 564, 612, 793, 1155
Bsp1720I GCTNAGC 1 cut(s) 873
Bsp19I CCATGG 1 cut(s) 164
BspACI CCGC 5 cut(s) 128, 755, 767, 785, 788
BspANI GGCC 1 cut(s) 453
BspCNI CTCAG 2 cut(s) 490, 865
BspDI ATCGAT 1 cut(s) 225
BspFNI CGCG 2 cut(s) 401, 755
BspHI TCATGA 1 cut(s) 738
BspLI GGNNCC 1 cut(s) 22
BspPI GGATC 4 cut(s) 261, 479, 514, 788
BspT107I GGYRCC 1 cut(s) 20
BspTNI GGTCTC 1 cut(s) 699
BsrI ACTGG 2 cut(s) 109, 235
BssECI CCNNGG 4 cut(s) 164, 448, 710, 1093
BssMI GATC 9 cut(s) 214, 222, 266, 484, 519, 564, 612, 793, 1155
BssNI GRCGYC 3 cut(s) 21, 180, 252
BssT1I CCWWGG 3 cut(s) 164, 448, 1093
Bst2UI CCWGG 3 cut(s) 205, 316, 711
Bst4CI ACNGT 3 cut(s) 347, 492, 583
Bst6I CTCTTC 1 cut(s) 555
BstACI GRCGYC 3 cut(s) 21, 180, 252
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 2 cut(s) 498, 873
BstDSI CCRYGG 1 cut(s) 164
BstF5I GGATG 2 cut(s) 217, 231
BstFNI CGCG 2 cut(s) 401, 755
BstH2I RGCGCY 1 cut(s) 24
BstHHI GCGC 2 cut(s) 23, 403
BstKTI GATC 9 cut(s) 217, 225, 269, 487, 522, 567, 615, 796, 1158
BstMAI GTCTC 3 cut(s) 250, 428, 699
BstMBI GATC 9 cut(s) 214, 222, 266, 484, 519, 564, 612, 793, 1155
BstMCI CGRYCG 1 cut(s) 225
BstMWI GCNNNNNNNGC 4 cut(s) 29, 78, 514, 752
BstNI CCWGG 3 cut(s) 205, 316, 711
BstNSI RCATGY 2 cut(s) 302, 419
BstSCI CCNGG 4 cut(s) 154, 203, 314, 709
BstUI CGCG 2 cut(s) 401, 755
BstV1I GCAGC 3 cut(s) 19, 683, 784
BstV2I GAAGAC 2 cut(s) 264, 295
BstX2I RGATCY 2 cut(s) 266, 519
BstYI RGATCY 2 cut(s) 266, 519
Bsu15I ATCGAT 1 cut(s) 225
BsuRI GGCC 1 cut(s) 453
BsuTUI ATCGAT 1 cut(s) 225
BtgI CCRYGG 1 cut(s) 164
BtgZI GCGATG 1 cut(s) 255
BtrI CACGTC 1 cut(s) 588
BtsCI GGATG 2 cut(s) 217, 231
BtsIMutI CAGTG 2 cut(s) 242, 495
Cac8I GCNNGC 1 cut(s) 19
CciI TCATGA 1 cut(s) 738
CfoI GCGC 2 cut(s) 23, 403
Cfr13I GGNCC 4 cut(s) 161, 318, 452, 473
ClaI ATCGAT 1 cut(s) 225
CseI GACGC 4 cut(s) 70, 169, 260, 761
Csp6I GTAC 2 cut(s) 1141, 1160
CviAII CATG 6 cut(s) 165, 299, 416, 610, 686, 739
CviQI GTAC 2 cut(s) 1141, 1160
DdeI CTNAG 2 cut(s) 498, 873
DinI GGCGCC 1 cut(s) 22
DpnI GATC 9 cut(s) 216, 224, 268, 486, 521, 566, 614, 795, 1157
DpnII GATC 9 cut(s) 214, 222, 266, 484, 519, 564, 612, 793, 1155
DraIII CACNNNGTG 1 cut(s) 158
DrdI GACNNNNNNGTC 1 cut(s) 420
DseDI GACNNNNNNGTC 1 cut(s) 420
Eam1104I CTCTTC 1 cut(s) 555
EarI CTCTTC 1 cut(s) 555
EciI GGCGGA 1 cut(s) 143
Eco130I CCWWGG 3 cut(s) 164, 448, 1093
Eco31I GGTCTC 1 cut(s) 699
Eco32I GATATC 1 cut(s) 838
Eco47I GGWCC 3 cut(s) 161, 318, 473
EcoRII CCWGG 3 cut(s) 203, 314, 709
EcoRV GATATC 1 cut(s) 838
EcoT14I CCWWGG 3 cut(s) 164, 448, 1093
EgeI GGCGCC 1 cut(s) 22
EheI GGCGCC 1 cut(s) 22
ErhI CCWWGG 3 cut(s) 164, 448, 1093
FaeI CATG 6 cut(s) 168, 302, 419, 613, 689, 742
FaqI GGGAC 2 cut(s) 448, 667
FatI CATG 6 cut(s) 164, 298, 415, 609, 685, 738
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 4 cut(s) 33, 672, 773, 786
FokI GGATG 2 cut(s) 204, 218
Fsp4HI GCNGC 4 cut(s) 33, 672, 773, 786
FspBI CTAG 1 cut(s) 869
GlaI GCGC 2 cut(s) 22, 402
GluI GCNGC 4 cut(s) 33, 672, 773, 786
GsuI CTGGAG 1 cut(s) 410
HaeII RGCGCY 1 cut(s) 24
HaeIII GGCC 1 cut(s) 453
HapII CCGG 1 cut(s) 156
HgaI GACGC 4 cut(s) 70, 169, 260, 761
HhaI GCGC 2 cut(s) 23, 403
Hin1I GRCGYC 3 cut(s) 21, 180, 252
Hin1II CATG 6 cut(s) 168, 302, 419, 613, 689, 742
Hin6I GCGC 2 cut(s) 21, 401
HinP1I GCGC 2 cut(s) 21, 401
HincII GTYRAC 1 cut(s) 469
HindII GTYRAC 1 cut(s) 469
HindIII AAGCTT 4 cut(s) 70, 272, 723, 998
HinfI GANTC 6 cut(s) 108, 133, 329, 655, 865, 979
HpaII CCGG 1 cut(s) 156
HphI GGTGA 2 cut(s) 304, 451
Hpy166II GTNNAC 3 cut(s) 175, 469, 495
Hpy188I TCNGA 8 cut(s) 29, 116, 148, 222, 250, 334, 413, 751
Hpy188III TCNNGA 9 cut(s) 76, 326, 427, 482, 523, 568, 739, 1102, 1153
Hpy8I GTNNAC 3 cut(s) 175, 469, 495
Hpy99I CGWCG 6 cut(s) 53, 86, 116, 185, 192, 254
HpyAV CCTTC 4 cut(s) 683, 725, 902, 1043
HpyCH4III ACNGT 3 cut(s) 347, 492, 583
HpyCH4IV ACGT 4 cut(s) 309, 375, 465, 587
HpyCH4V TGCA 4 cut(s) 517, 671, 825, 988
HpyF10VI GCNNNNNNNGC 4 cut(s) 29, 78, 514, 752
HpyF3I CTNAG 2 cut(s) 498, 873
HpySE526I ACGT 4 cut(s) 309, 375, 465, 587
Hsp92I GRCGYC 3 cut(s) 21, 180, 252
Hsp92II CATG 6 cut(s) 168, 302, 419, 613, 689, 742
HspAI GCGC 2 cut(s) 21, 401
KasI GGCGCC 1 cut(s) 20
Kzo9I GATC 9 cut(s) 214, 222, 266, 484, 519, 564, 612, 793, 1155
LmnI GCTCC 4 cut(s) 29, 372, 714, 769
Lsp1109I GCAGC 3 cut(s) 19, 683, 784
LweI GCATC 1 cut(s) 862
MaeI CTAG 1 cut(s) 869
MaeII ACGT 4 cut(s) 309, 375, 465, 587
MaeIII GTNAC 6 cut(s) 100, 292, 310, 371, 479, 583
MalI GATC 9 cut(s) 216, 224, 268, 486, 521, 566, 614, 795, 1157
MboI GATC 9 cut(s) 214, 222, 266, 484, 519, 564, 612, 793, 1155
MfeI CAATTG 1 cut(s) 983
MflI RGATCY 2 cut(s) 266, 519
MluCI AATT 3 cut(s) 526, 916, 983
Mly113I GGCGCC 1 cut(s) 21
MlyI GAGTC 1 cut(s) 649
MmeI TCCRAC 5 cut(s) 94, 171, 273, 436, 1134
MseI TTAA 3 cut(s) 284, 819, 972
MspI CCGG 1 cut(s) 156
MspR9I CCNGG 4 cut(s) 156, 205, 316, 711
MunI CAATTG 1 cut(s) 983
MvaI CCWGG 3 cut(s) 205, 316, 711
MvnI CGCG 2 cut(s) 401, 755
MwoI GCNNNNNNNGC 4 cut(s) 29, 78, 514, 752
NarI GGCGCC 1 cut(s) 21
NciI CCSGG 1 cut(s) 156
NcoI CCATGG 1 cut(s) 164
NdeII GATC 9 cut(s) 214, 222, 266, 484, 519, 564, 612, 793, 1155
NlaIII CATG 6 cut(s) 168, 302, 419, 613, 689, 742
NlaIV GGNNCC 1 cut(s) 22
NmeAIII GCCGAG 1 cut(s) 534
NmuCI GTSAC 4 cut(s) 292, 310, 479, 583
NspI RCATGY 2 cut(s) 302, 419
PagI TCATGA 1 cut(s) 738
PciI ACATGT 2 cut(s) 298, 415
PcsI WCGNNNNNNNCGW 1 cut(s) 193
PfeI GAWTC 5 cut(s) 108, 133, 329, 865, 979
PflFI GACNNNGTC 2 cut(s) 477, 589
PkrI GCNGC 4 cut(s) 34, 673, 774, 787
Ple19I CGATCG 1 cut(s) 225
PleI GAGTC 1 cut(s) 649
PluTI GGCGCC 1 cut(s) 24
PpsI GAGTC 1 cut(s) 649
PscI ACATGT 2 cut(s) 298, 415
Psp1406I AACGTT 2 cut(s) 375, 465
Psp6I CCWGG 3 cut(s) 203, 314, 709
PspGI CCWGG 3 cut(s) 203, 314, 709
PspN4I GGNNCC 1 cut(s) 22
PspPI GGNCC 4 cut(s) 161, 318, 452, 473
PsrI GAACNNNNNNTAC 2 cut(s) 1133, 1165
PsuI RGATCY 2 cut(s) 266, 519
PsyI GACNNNGTC 2 cut(s) 477, 589
PvuI CGATCG 1 cut(s) 225
RsaI GTAC 2 cut(s) 1142, 1161
RsaNI GTAC 2 cut(s) 1141, 1160
SaqAI TTAA 3 cut(s) 284, 819, 972
SatI GCNGC 4 cut(s) 33, 672, 773, 786
Sau3AI GATC 9 cut(s) 214, 222, 266, 484, 519, 564, 612, 793, 1155
Sau96I GGNCC 4 cut(s) 161, 318, 452, 473
SchI GAGTC 1 cut(s) 649
ScrFI CCNGG 4 cut(s) 156, 205, 316, 711
SfaNI GCATC 1 cut(s) 862
SfoI GGCGCC 1 cut(s) 22
SinI GGWCC 3 cut(s) 161, 318, 473
SmlI CTYRAG 1 cut(s) 1001
SmoI CTYRAG 1 cut(s) 1001
Sse9I AATT 3 cut(s) 526, 916, 983
SsiI CCGC 5 cut(s) 128, 755, 767, 785, 788
SspDI GGCGCC 1 cut(s) 20
SspMI CTAG 1 cut(s) 869
StyD4I CCNGG 4 cut(s) 154, 203, 314, 709
StyI CCWWGG 3 cut(s) 164, 448, 1093
TaaI ACNGT 3 cut(s) 347, 492, 583
TaiI ACGT 4 cut(s) 312, 378, 468, 590
TaqI TCGA 3 cut(s) 48, 75, 225
TasI AATT 3 cut(s) 526, 916, 983
TatI WGTACW 1 cut(s) 1140
TauI GCSGC 1 cut(s) 788
TfiI GAWTC 5 cut(s) 108, 133, 329, 865, 979
Tru1I TTAA 3 cut(s) 284, 819, 972
Tru9I TTAA 3 cut(s) 284, 819, 972
TscAI CASTG 2 cut(s) 242, 502
TseFI GTSAC 4 cut(s) 292, 310, 479, 583
TseI GCWGC 3 cut(s) 32, 671, 772
Tsp45I GTSAC 4 cut(s) 292, 310, 479, 583
TspDTI ATGAA 6 cut(s) 72, 656, 702, 727, 953, 1082
TspGWI ACGGA 4 cut(s) 359, 486, 687, 786
TspRI CASTG 2 cut(s) 242, 502
Tth111I GACNNNGTC 2 cut(s) 477, 589
VpaK11BI GGWCC 3 cut(s) 161, 318, 473
XapI RAATTY 2 cut(s) 526, 916
XceI RCATGY 2 cut(s) 302, 419
XmiI GTMKAC 1 cut(s) 174
XspI CTAG 1 cut(s) 869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.