Rmu_sc0020102.1_g000006

3-oxo-Delta(4,5)-steroid 5-beta-reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0020102.1
OG0000626
Physical Location & Seq
Reverse (-)
36250 .. 37720
1471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0020102.1_g000006.1.cds

Sequence Viewer

Length: 1308 bp
atggtcaccaggtcacattggattcagattttatctgccctcatctcatctaccggcctttctcaccccctcaccctctccactccttatcttcctcctttgctcttctcctcctccgacgatttcccagaaaccatgagctggtggtgggccggagccgttggcgctgccaagagaaaattagtagaagatgaagggccgcgaacgccgcagagcgtggggttagtgatcggcgtgacgggaatagtcggcaacagcctcgccgagatcctccctctctccgacacccccggcggtccatggaaggtctacggcgtcgcccgccgtccccggcccaactggaacgccgatcaccccgtcgagtacatccagtgcgacgtctccgacgccgaggacgccaaggccaagctcacgccgttgaccgacgtcactcacctcttctacgtcacctggtccagccgatccaccgaggccgagaattgcgaggccaacgccgtgatgctgcgcaacgtcttgagctccgtgatcccaaacgcgccgaatctgaagcacatatgcctccagacgggaggcaaacactacatcgggccgttcgagtcgtgggggaagatcgagccgcacgacacgccgttcacggaggatctgccccggttgagctaccagaatttctactacacactcgaagatatattgttcgaagaggtggagaagaaagaggagctgacttggtcggtgcaccgacccgacaccattttcgggttttcgccttatagcttgatgaacattgtaggcactctgtgtgtgtatgctgctatatgcaaacatgagggtctgccactgagatttccgggaaccaaagaggcgtggaattgcttgtcggcagcttcggatgcggatttgatagcggagcagcagatatgggctgcagtggatccttatgctcgaaacgaagccttcaatatcaacaatgcggatgtgttcaggtggaagcatttctggaaggtgttggctgagaatttcgggatagaggaattcgggtttgaggagaagggggagaggttaagcttggagaagttgatggaggggaagaatggagtgtgggaggagatagtgaaggagaatcagctgctgccgacgaagctggaggaggttggagtgtggtggtttgtggatactgtgtttagtggggaaagtaagtttggttgtatgaacaagagtaaagaacatgggtttgtggggtttaggaactccaggaattcctttgttacttggattgataaaatgaaagcttacaagcttgtgccttga

Protein Analysis

435

Amino Acids

49.13

Weight (kDa)

5.28

Isoelectric Point (pI)

40.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000626)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24220 AT4G24220
fragaria_vesca FvH4_2g04580 FvH4_2g04580 FvH4_2g04580 FvH4_4g06071 FvH4_5g25130
malus_domestica MD05G1094500.v1.1 MD05G1108300.v1.1 MD08G1088400.v1.1 MD15G1073200.v1.1 MD15G1073300.v1.1 MD15G1073400.v1.1 MD15G1073500.v1.1 MD15G1073600.v1.1
prunus_persica Prupe.1G426100_v2.0.a1 Prupe.1G426100_v2.0.a1 Prupe.1G426200_v2.0.a1 Prupe.1G426300_v2.0.a1 Prupe.1G426400_v2.0.a1 Prupe.1G426400_v2.0.a1 Prupe.8G139400_v2.0.a1 Prupe.8G139400_v2.0.a1
pyrus_communis pycom05g09350 pycom08g07190 pycom13g22780 pycom15g06840 pycom15g06850 pycom15g06870
rosa_chinensis RchiOBHm_Chr6g0254161 RchiOBHm_Chr6g0254181 RchiOBHm_Chr6g0254191 RchiOBHm_Chr6g0309171 RchiOBHm_Chr6g0309181 RchiOBHm_Chr6g0309191 RchiOBHm_Chr6g0309201
rosa_laevigata RLG00000010582 RLG00000010583 RLG00000014937 RLG00000014938
rosa_multiflora Rmu_co8060540.1_g000001 Rmu_co8332663.1_g000001 Rmu_sc0000010.1_g000017 Rmu_sc0003711.1_g000008 Rmu_sc0016971.1_g000003 Rmu_sc0020102.1_g000006 Rmu_sc0020102.1_g000008
rosa_roxburghii Rroxscaffold_7G00159570 Rroxscaffold_7G00159580 Rroxscaffold_7G00159590 Rroxscaffold_7G00211100
rosa_rugosa Rorug05G0550400 Rorug06G0370100 Rorug06G0370300
rosa_samantha Rh6AG066900 Rh6AG483200 Rh6AG483300 Rh6AG483400 Rh6BG059800 Rh6BG060000 Rh6BG492200 Rh6BG492300 Rh6BG492500 Rh6CG059700 Rh6CG059800 Rh6CG496900 Rh6CG497000 Rh6CG497200 Rh6CG497400 Rh6DG056700 Rh6DG056800 Rh6DG483800 Rh6DG484000
rosa_wichuraiana Rw6G005860 Rw6G005870 Rw6G042070 Rw6G042080 Rw6G042090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 381, 429
Acc16I TGCGCA 1 cut(s) 506
AccB7I CCANNNNNTGG 1 cut(s) 141
AccI GTMKAC 1 cut(s) 309
AccII CGCG 2 cut(s) 202, 536
AciI CCGC 8 cut(s) 200, 209, 294, 322, 617, 893, 905, 971
AclWI GGATC 6 cut(s) 262, 456, 520, 648, 926, 939
AcsI RAATTY 4 cut(s) 664, 1015, 1031, 1255
AcuI CTGAAG 1 cut(s) 566
AcyI GRCGYC 5 cut(s) 315, 378, 387, 396, 426
AdeI CACNNNGTG 1 cut(s) 798
AfaI GTAC 1 cut(s) 365
AfiI CCNNNNNNNGG 3 cut(s) 141, 565, 756
AgsI TTSAA 1 cut(s) 958
AjnI CCWGG 3 cut(s) 8, 449, 1250
AjuI GAANNNNNNNTTGG 2 cut(s) 1182, 1214
Alw21I GWGCWC 2 cut(s) 521, 738
Alw26I GTCTC 1 cut(s) 385
Alw44I GTGCAC 1 cut(s) 734
AlwI GGATC 6 cut(s) 262, 456, 520, 648, 926, 939
AlwNI CAGNNNCTG 1 cut(s) 1129
AoxI GGCC 8 cut(s) 55, 150, 197, 332, 402, 471, 486, 587
ApaLI GTGCAC 1 cut(s) 734
ApeKI GCWGC 8 cut(s) 167, 502, 809, 881, 910, 923, 1126, 1129
ApoI RAATTY 4 cut(s) 664, 1015, 1031, 1255
ArsI GACNNNNNNTTYG 2 cut(s) 737, 769
Asp700I GAANNNNTTC 1 cut(s) 992
AspLEI GCGC 3 cut(s) 167, 507, 538
AspS9I GGNCC 6 cut(s) 150, 197, 296, 333, 453, 587
AsuC2I CCSGG 4 cut(s) 291, 331, 649, 849
AsuHPI GGTGA 5 cut(s) 56, 64, 344, 425, 439
AsuII TTCGAA 1 cut(s) 696
AvaII GGWCC 2 cut(s) 296, 453
BaeGI GKGCMC 1 cut(s) 738
BamHI GGATCC 1 cut(s) 931
BanII GRGCYC 1 cut(s) 521
Bbv12I GWGCWC 2 cut(s) 521, 738
BbvI GCAGC 8 cut(s) 154, 489, 796, 893, 910, 922, 1113, 1116
BccI CCATC 1 cut(s) 1072
BceAI ACGGC 7 cut(s) 143, 309, 328, 400, 479, 574, 613
BcgI CGANNNNNNTGC 2 cut(s) 241, 275
BciT130I CCWGG 3 cut(s) 10, 451, 1252
BciVI GTATCC 1 cut(s) 1165
BcnI CCSGG 4 cut(s) 291, 331, 649, 849
BcoDI GTCTC 1 cut(s) 385
BfmI CTRYAG 1 cut(s) 924
BfoI RGCGCY 1 cut(s) 168
BfuI GTATCC 1 cut(s) 1165
Bme1390I CCNGG 7 cut(s) 10, 291, 331, 451, 649, 849, 1252
Bme18I GGWCC 2 cut(s) 296, 453
BmgT120I GGNCC 6 cut(s) 150, 197, 296, 333, 453, 587
BmiI GGNNCC 3 cut(s) 157, 853, 933
BmrFI CCNGG 7 cut(s) 10, 291, 331, 451, 649, 849, 1252
BmsI GCATC 2 cut(s) 489, 880
BoxI GACNNNNGTC 1 cut(s) 425
BpmI CTGGAG 3 cut(s) 545, 1163, 1234
Bpu14I TTCGAA 1 cut(s) 696
BpuEI CTTGAG 1 cut(s) 535
BpuMI CCSGG 4 cut(s) 291, 331, 649, 849
BsaHI GRCGYC 5 cut(s) 315, 378, 387, 396, 426
BsaJI CCNNGG 7 cut(s) 289, 299, 329, 390, 399, 468, 647
Bsc4I CCNNNNNNNGG 3 cut(s) 141, 565, 756
Bse118I RCCGGY 1 cut(s) 53
Bse1I ACTGG 2 cut(s) 344, 370
BseBI CCWGG 3 cut(s) 10, 451, 1252
BseDI CCNNGG 7 cut(s) 289, 299, 329, 390, 399, 468, 647
BseGI GGATG 3 cut(s) 366, 895, 979
BseLI CCNNNNNNNGG 3 cut(s) 141, 565, 756
BseMII CTCAG 2 cut(s) 830, 1002
BseNI ACTGG 2 cut(s) 344, 370
BseRI GAGGAG 6 cut(s) 100, 103, 731, 1058, 1118, 1160
BseSI GKGCMC 1 cut(s) 738
BseXI GCAGC 8 cut(s) 154, 489, 796, 893, 910, 922, 1113, 1116
Bsh1236I CGCG 2 cut(s) 202, 536
BshFI GGCC 8 cut(s) 57, 152, 199, 334, 404, 473, 488, 589
BsiHKAI GWGCWC 2 cut(s) 521, 738
BsiSI CCGG 6 cut(s) 54, 153, 291, 331, 649, 848
BslFI GGGAC 1 cut(s) 312
BslI CCNNNNNNNGG 3 cut(s) 141, 565, 756
BsmAI GTCTC 1 cut(s) 385
BsmBI CGTCTC 1 cut(s) 385
BsmFI GGGAC 1 cut(s) 312
BsnI GGCC 8 cut(s) 57, 152, 199, 334, 404, 473, 488, 589
Bsp119I TTCGAA 1 cut(s) 696
Bsp1286I GDGCHC 2 cut(s) 521, 738
Bsp143I GATC 8 cut(s) 228, 267, 349, 461, 525, 609, 640, 931
Bsp19I CCATGG 1 cut(s) 299
BspACI CCGC 8 cut(s) 200, 209, 294, 322, 617, 893, 905, 971
BspANI GGCC 8 cut(s) 57, 152, 199, 334, 404, 473, 488, 589
BspCNI CTCAG 2 cut(s) 831, 1003
BspFNI CGCG 2 cut(s) 202, 536
BspLI GGNNCC 3 cut(s) 157, 853, 933
BspMAI CTGCAG 1 cut(s) 928
BspPI GGATC 6 cut(s) 262, 456, 520, 648, 926, 939
BspQI GCTCTTC 1 cut(s) 110
BspT104I TTCGAA 1 cut(s) 696
BsrFI RCCGGY 1 cut(s) 53
BsrI ACTGG 2 cut(s) 344, 370
BssAI RCCGGY 1 cut(s) 53
BssECI CCNNGG 7 cut(s) 289, 299, 329, 390, 399, 468, 647
BssMI GATC 8 cut(s) 228, 267, 349, 461, 525, 609, 640, 931
BssNI GRCGYC 5 cut(s) 315, 378, 387, 396, 426
BssT1I CCWWGG 2 cut(s) 299, 399
Bst2UI CCWGG 3 cut(s) 10, 451, 1252
Bst4CI ACNGT 1 cut(s) 1177
Bst6I CTCTTC 3 cut(s) 110, 443, 693
BstACI GRCGYC 5 cut(s) 315, 378, 387, 396, 426
BstBI TTCGAA 1 cut(s) 696
BstC8I GCNNGC 1 cut(s) 322
BstDEI CTNAG 2 cut(s) 839, 1011
BstDSI CCRYGG 1 cut(s) 299
BstEII GGTNACC 1 cut(s) 4
BstF5I GGATG 3 cut(s) 366, 895, 979
BstFNI CGCG 2 cut(s) 202, 536
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 3 cut(s) 167, 507, 538
BstKTI GATC 8 cut(s) 231, 270, 352, 464, 528, 612, 643, 934
BstMAI GTCTC 1 cut(s) 385
BstMBI GATC 8 cut(s) 228, 267, 349, 461, 525, 609, 640, 931
BstMWI GCNNNNNNNGC 8 cut(s) 164, 205, 208, 321, 395, 625, 890, 1138
BstNI CCWGG 3 cut(s) 10, 451, 1252
BstPAI GACNNNNGTC 1 cut(s) 425
BstPI GGTNACC 1 cut(s) 4
BstSCI CCNGG 7 cut(s) 8, 289, 329, 449, 647, 847, 1250
BstSFI CTRYAG 1 cut(s) 924
BstSLI GKGCMC 1 cut(s) 738
BstUI CGCG 2 cut(s) 202, 536
BstV1I GCAGC 8 cut(s) 154, 489, 796, 893, 910, 922, 1113, 1116
BstX2I RGATCY 3 cut(s) 267, 640, 931
BstYI RGATCY 3 cut(s) 267, 640, 931
BsuI GTATCC 1 cut(s) 1165
BsuRI GGCC 8 cut(s) 57, 152, 199, 334, 404, 473, 488, 589
BtgI CCRYGG 1 cut(s) 299
BtsCI GGATG 3 cut(s) 366, 895, 979
BtsI GCAGTG 1 cut(s) 933
BtsIMutI CAGTG 3 cut(s) 377, 836, 933
Cac8I GCNNGC 1 cut(s) 322
CaiI CAGNNNCTG 1 cut(s) 1129
CfoI GCGC 3 cut(s) 167, 507, 538
Cfr10I RCCGGY 1 cut(s) 53
Cfr13I GGNCC 6 cut(s) 150, 197, 296, 333, 453, 587
CseI GACGC 3 cut(s) 304, 395, 404
CsiI ACCWGGT 2 cut(s) 8, 449
Csp6I GTAC 1 cut(s) 364
CviAII CATG 4 cut(s) 136, 300, 824, 1226
CviQI GTAC 1 cut(s) 364
DdeI CTNAG 2 cut(s) 839, 1011
DpnI GATC 8 cut(s) 230, 269, 351, 463, 527, 611, 642, 933
DpnII GATC 8 cut(s) 228, 267, 349, 461, 525, 609, 640, 931
DraIII CACNNNGTG 1 cut(s) 798
Eam1104I CTCTTC 3 cut(s) 110, 443, 693
EarI CTCTTC 3 cut(s) 110, 443, 693
Ecl136II GAGCTC 1 cut(s) 519
Eco130I CCWWGG 2 cut(s) 299, 399
Eco24I GRGCYC 1 cut(s) 521
Eco47I GGWCC 2 cut(s) 296, 453
Eco53kI GAGCTC 1 cut(s) 519
Eco57I CTGAAG 1 cut(s) 566
Eco91I GGTNACC 1 cut(s) 4
EcoICRI GAGCTC 1 cut(s) 519
EcoO65I GGTNACC 1 cut(s) 4
EcoRI GAATTC 2 cut(s) 1031, 1255
EcoRII CCWGG 3 cut(s) 8, 449, 1250
EcoT14I CCWWGG 2 cut(s) 299, 399
EcoT38I GRGCYC 1 cut(s) 521
ErhI CCWWGG 2 cut(s) 299, 399
Esp3I CGTCTC 1 cut(s) 385
FaeI CATG 4 cut(s) 139, 303, 827, 1229
FaqI GGGAC 1 cut(s) 312
FatI CATG 4 cut(s) 135, 299, 823, 1225
FauI CCCGC 1 cut(s) 329
FauNDI CATATG 1 cut(s) 554
FblI GTMKAC 1 cut(s) 309
FokI GGATG 3 cut(s) 353, 902, 986
FriOI GRGCYC 1 cut(s) 521
FspI TGCGCA 1 cut(s) 506
GlaI GCGC 3 cut(s) 166, 506, 537
GsuI CTGGAG 3 cut(s) 545, 1163, 1234
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 8 cut(s) 57, 152, 199, 334, 404, 473, 488, 589
HapII CCGG 6 cut(s) 54, 153, 291, 331, 649, 848
HgaI GACGC 3 cut(s) 304, 395, 404
HhaI GCGC 3 cut(s) 167, 507, 538
Hin1I GRCGYC 5 cut(s) 315, 378, 387, 396, 426
Hin1II CATG 4 cut(s) 139, 303, 827, 1229
Hin6I GCGC 3 cut(s) 165, 505, 536
HinP1I GCGC 3 cut(s) 165, 505, 536
HincII GTYRAC 1 cut(s) 420
HindII GTYRAC 1 cut(s) 420
HindIII AAGCTT 3 cut(s) 1063, 1287, 1295
HinfI GANTC 4 cut(s) 22, 541, 596, 1120
HpaII CCGG 6 cut(s) 54, 153, 291, 331, 649, 848
HphI GGTGA 5 cut(s) 56, 64, 344, 425, 439
Hpy166II GTNNAC 4 cut(s) 310, 420, 633, 736
Hpy188I TCNGA 6 cut(s) 27, 118, 283, 385, 546, 889
Hpy188III TCNNGA 4 cut(s) 514, 562, 997, 1021
Hpy8I GTNNAC 4 cut(s) 310, 420, 633, 736
Hpy99I CGWCG 7 cut(s) 122, 320, 362, 380, 389, 428, 1138
HpyAV CCTTC 6 cut(s) 188, 298, 964, 994, 1042, 1108
HpyCH4III ACNGT 1 cut(s) 1177
HpyCH4IV ACGT 4 cut(s) 378, 426, 444, 510
HpyCH4V TGCA 3 cut(s) 736, 819, 926
HpyF10VI GCNNNNNNNGC 8 cut(s) 164, 205, 208, 321, 395, 625, 890, 1138
HpyF3I CTNAG 2 cut(s) 839, 1011
HpySE526I ACGT 4 cut(s) 378, 426, 444, 510
Hsp92I GRCGYC 5 cut(s) 315, 378, 387, 396, 426
Hsp92II CATG 4 cut(s) 139, 303, 827, 1229
HspAI GCGC 3 cut(s) 165, 505, 536
Kzo9I GATC 8 cut(s) 228, 267, 349, 461, 525, 609, 640, 931
LguI GCTCTTC 1 cut(s) 110
LmnI GCTCC 4 cut(s) 155, 524, 718, 907
Lsp1109I GCAGC 8 cut(s) 154, 489, 796, 893, 910, 922, 1113, 1116
LweI GCATC 2 cut(s) 489, 880
MabI ACCWGGT 2 cut(s) 8, 449
MaeII ACGT 4 cut(s) 378, 426, 444, 510
MaeIII GTNAC 6 cut(s) 4, 12, 235, 427, 445, 1264
MalI GATC 8 cut(s) 230, 269, 351, 463, 527, 611, 642, 933
MboI GATC 8 cut(s) 228, 267, 349, 461, 525, 609, 640, 931
MboII GAAGA 9 cut(s) 83, 97, 200, 430, 619, 695, 710, 721, 1099
MflI RGATCY 3 cut(s) 267, 640, 931
MhlI GDGCHC 2 cut(s) 521, 738
MluCI AATT 7 cut(s) 179, 478, 664, 868, 1015, 1031, 1255
MlyI GAGTC 1 cut(s) 605
MmeI TCCRAC 4 cut(s) 141, 306, 408, 1132
MroXI GAANNNNTTC 1 cut(s) 992
MseI TTAA 1 cut(s) 1061
MspA1I CMGCKG 1 cut(s) 1126
MspI CCGG 6 cut(s) 54, 153, 291, 331, 649, 848
MspR9I CCNGG 7 cut(s) 10, 291, 331, 451, 649, 849, 1252
MvaI CCWGG 3 cut(s) 10, 451, 1252
MvnI CGCG 2 cut(s) 202, 536
MwoI GCNNNNNNNGC 8 cut(s) 164, 205, 208, 321, 395, 625, 890, 1138
NciI CCSGG 4 cut(s) 291, 331, 649, 849
NcoI CCATGG 1 cut(s) 299
NdeI CATATG 1 cut(s) 554
NdeII GATC 8 cut(s) 228, 267, 349, 461, 525, 609, 640, 931
NlaIII CATG 4 cut(s) 139, 303, 827, 1229
NlaIV GGNNCC 3 cut(s) 157, 853, 933
NmeAIII GCCGAG 3 cut(s) 289, 415, 499
NmuCI GTSAC 5 cut(s) 4, 12, 235, 427, 445
NsbI TGCGCA 1 cut(s) 506
NspV TTCGAA 1 cut(s) 696
PciSI GCTCTTC 1 cut(s) 110
PcsI WCGNNNNNNNCGW 2 cut(s) 591, 618
PdmI GAANNNNTTC 1 cut(s) 992
PfeI GAWTC 3 cut(s) 22, 541, 1120
PflFI GACNNNGTC 1 cut(s) 727
PflMI CCANNNNNTGG 1 cut(s) 141
PfoI TCCNGGA 2 cut(s) 847, 1250
PleI GAGTC 1 cut(s) 604
PpsI GAGTC 1 cut(s) 604
PshAI GACNNNNGTC 1 cut(s) 425
Psp124BI GAGCTC 1 cut(s) 521
Psp6I CCWGG 3 cut(s) 8, 449, 1250
PspEI GGTNACC 1 cut(s) 4
PspGI CCWGG 3 cut(s) 8, 449, 1250
PspN4I GGNNCC 3 cut(s) 157, 853, 933
PspPI GGNCC 6 cut(s) 150, 197, 296, 333, 453, 587
PstI CTGCAG 1 cut(s) 928
PstNI CAGNNNCTG 1 cut(s) 1129
PsuI RGATCY 3 cut(s) 267, 640, 931
PsyI GACNNNGTC 1 cut(s) 727
PvuII CAGCTG 1 cut(s) 1126
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
SacI GAGCTC 1 cut(s) 521
SapI GCTCTTC 1 cut(s) 110
SaqAI TTAA 1 cut(s) 1061
Sau3AI GATC 8 cut(s) 228, 267, 349, 461, 525, 609, 640, 931
Sau96I GGNCC 6 cut(s) 150, 197, 296, 333, 453, 587
SchI GAGTC 1 cut(s) 605
ScrFI CCNGG 7 cut(s) 10, 291, 331, 451, 649, 849, 1252
SduI GDGCHC 2 cut(s) 521, 738
SexAI ACCWGGT 2 cut(s) 8, 449
SfaNI GCATC 2 cut(s) 489, 880
SfcI CTRYAG 1 cut(s) 924
SfuI TTCGAA 1 cut(s) 696
SinI GGWCC 2 cut(s) 296, 453
SmlI CTYRAG 1 cut(s) 514
SmoI CTYRAG 1 cut(s) 514
Sse9I AATT 7 cut(s) 179, 478, 664, 868, 1015, 1031, 1255
SsiI CCGC 8 cut(s) 200, 209, 294, 322, 617, 893, 905, 971
SstI GAGCTC 1 cut(s) 521
StyD4I CCNGG 7 cut(s) 8, 289, 329, 449, 647, 847, 1250
StyI CCWWGG 2 cut(s) 299, 399
TaaI ACNGT 1 cut(s) 1177
TaiI ACGT 4 cut(s) 381, 429, 447, 513
TaqI TCGA 6 cut(s) 360, 594, 612, 681, 696, 943
TaqII GACCGA 1 cut(s) 437
TasI AATT 7 cut(s) 179, 478, 664, 868, 1015, 1031, 1255
TatI WGTACW 1 cut(s) 363
TauI GCSGC 3 cut(s) 202, 211, 619
TfiI GAWTC 3 cut(s) 22, 541, 1120
Tru1I TTAA 1 cut(s) 1061
Tru9I TTAA 1 cut(s) 1061
TscAI CASTG 3 cut(s) 377, 843, 933
TseFI GTSAC 5 cut(s) 4, 12, 235, 427, 445
TseI GCWGC 8 cut(s) 167, 502, 809, 881, 910, 923, 1126, 1129
Tsp45I GTSAC 5 cut(s) 4, 12, 235, 427, 445
TspDTI ATGAA 4 cut(s) 207, 794, 1223, 1298
TspGWI ACGGA 2 cut(s) 511, 650
TspRI CASTG 3 cut(s) 377, 843, 933
Tth111I GACNNNGTC 1 cut(s) 727
Van91I CCANNNNNTGG 1 cut(s) 141
VneI GTGCAC 1 cut(s) 734
VpaK11BI GGWCC 2 cut(s) 296, 453
XapI RAATTY 4 cut(s) 664, 1015, 1031, 1255
XmiI GTMKAC 1 cut(s) 309
XmnI GAANNNNTTC 1 cut(s) 992
ZraI GACGTC 2 cut(s) 379, 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.