3-oxo-Delta(4,5)-steroid 5-beta-reductase-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1058 |
| AarI | CACCTGC | 1 cut(s) | 173 |
| Acc36I | ACCTGC | 2 cut(s) | 173, 714 |
| AccI | GTMKAC | 1 cut(s) | 796 |
| AccII | CGCG | 1 cut(s) | 325 |
| AciI | CCGC | 1 cut(s) | 323 |
| AclWI | GGATC | 2 cut(s) | 228, 241 |
| AcsI | RAATTY | 4 cut(s) | 122, 308, 376, 1135 |
| AcuI | CTGAAG | 1 cut(s) | 406 |
| AfaI | GTAC | 4 cut(s) | 119, 161, 186, 1071 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 712, 834 |
| AflII | CTTAAG | 1 cut(s) | 190 |
| AflIII | ACRYGT | 2 cut(s) | 822, 994 |
| AgeI | ACCGGT | 1 cut(s) | 1066 |
| AjiI | CACGTC | 1 cut(s) | 140 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 557, 589 |
| AluBI | AGCT | 5 cut(s) | 223, 781, 876, 929, 1028 |
| AluI | AGCT | 5 cut(s) | 223, 781, 876, 929, 1028 |
| Alw26I | GTCTC | 2 cut(s) | 728, 1070 |
| AlwI | GGATC | 2 cut(s) | 228, 241 |
| AoxI | GGCC | 3 cut(s) | 202, 320, 462 |
| ApeKI | GCWGC | 3 cut(s) | 758, 781, 1100 |
| ApoI | RAATTY | 4 cut(s) | 122, 308, 376, 1135 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 82, 114 |
| AsiGI | ACCGGT | 1 cut(s) | 1066 |
| AspLEI | GCGC | 2 cut(s) | 327, 1011 |
| AspS9I | GGNCC | 2 cut(s) | 203, 463 |
| AsuHPI | GGTGA | 4 cut(s) | 263, 973, 1056, 1173 |
| AxyI | CCTNAGG | 1 cut(s) | 711 |
| BamHI | GGATCC | 1 cut(s) | 233 |
| BbvI | GCAGC | 3 cut(s) | 745, 768, 1087 |
| BccI | CCATC | 1 cut(s) | 827 |
| BclI | TGATCA | 2 cut(s) | 151, 178 |
| BcoDI | GTCTC | 2 cut(s) | 728, 1070 |
| BfaI | CTAG | 5 cut(s) | 762, 773, 887, 1121, 1190 |
| BfrI | CTTAAG | 1 cut(s) | 190 |
| BfuAI | ACCTGC | 2 cut(s) | 173, 714 |
| BisI | GCNGC | 4 cut(s) | 323, 759, 782, 1101 |
| BlsI | GCNGC | 4 cut(s) | 324, 760, 783, 1102 |
| BmgBI | CACGTC | 1 cut(s) | 140 |
| BmgT120I | GGNCC | 2 cut(s) | 203, 463 |
| BmiI | GGNNCC | 1 cut(s) | 235 |
| BmsI | GCATC | 3 cut(s) | 324, 547, 1087 |
| BpmI | CTGGAG | 1 cut(s) | 988 |
| BpuEI | CTTGAG | 4 cut(s) | 213, 527, 533, 1094 |
| BsaBI | GATNNNNATC | 2 cut(s) | 255, 1110 |
| BsaWI | WCCGGW | 2 cut(s) | 1066, 1164 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 712, 834 |
| Bse118I | RCCGGY | 1 cut(s) | 1066 |
| Bse1I | ACTGG | 3 cut(s) | 662, 971, 1038 |
| Bse21I | CCTNAGG | 1 cut(s) | 711 |
| Bse8I | GATNNNNATC | 2 cut(s) | 255, 1110 |
| BseJI | GATNNNNATC | 2 cut(s) | 255, 1110 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 712, 834 |
| BseMII | CTCAG | 2 cut(s) | 725, 860 |
| BseNI | ACTGG | 3 cut(s) | 662, 971, 1038 |
| BseRI | GAGGAG | 1 cut(s) | 489 |
| BseXI | GCAGC | 3 cut(s) | 745, 768, 1087 |
| Bsh1236I | CGCG | 1 cut(s) | 325 |
| BshFI | GGCC | 3 cut(s) | 204, 322, 464 |
| BshTI | ACCGGT | 1 cut(s) | 1066 |
| BsiSI | CCGG | 2 cut(s) | 1067, 1165 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 712, 834 |
| BsmAI | GTCTC | 2 cut(s) | 728, 1070 |
| BsmI | GAATGC | 2 cut(s) | 410, 642 |
| BsnI | GGCC | 3 cut(s) | 204, 322, 464 |
| Bsp143I | GATC | 4 cut(s) | 151, 178, 233, 256 |
| BspACI | CCGC | 1 cut(s) | 323 |
| BspANI | GGCC | 3 cut(s) | 204, 322, 464 |
| BspCNI | CTCAG | 2 cut(s) | 724, 861 |
| BspFNI | CGCG | 1 cut(s) | 325 |
| BspLI | GGNNCC | 1 cut(s) | 235 |
| BspMI | ACCTGC | 2 cut(s) | 173, 714 |
| BspPI | GGATC | 2 cut(s) | 228, 241 |
| BspTI | CTTAAG | 1 cut(s) | 190 |
| BsrFI | RCCGGY | 1 cut(s) | 1066 |
| BsrI | ACTGG | 3 cut(s) | 662, 971, 1038 |
| BssAI | RCCGGY | 1 cut(s) | 1066 |
| BssMI | GATC | 4 cut(s) | 151, 178, 233, 256 |
| Bst4CI | ACNGT | 3 cut(s) | 530, 536, 1087 |
| BstAFI | CTTAAG | 1 cut(s) | 190 |
| BstC8I | GCNNGC | 2 cut(s) | 462, 1176 |
| BstDEI | CTNAG | 2 cut(s) | 711, 869 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 710 |
| BstFNI | CGCG | 1 cut(s) | 325 |
| BstHHI | GCGC | 2 cut(s) | 327, 1011 |
| BstKTI | GATC | 4 cut(s) | 154, 181, 236, 259 |
| BstMAI | GTCTC | 2 cut(s) | 728, 1070 |
| BstMBI | GATC | 4 cut(s) | 151, 178, 233, 256 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 210, 457, 778 |
| BstNSI | RCATGY | 3 cut(s) | 826, 848, 998 |
| BstUI | CGCG | 1 cut(s) | 325 |
| BstV1I | GCAGC | 3 cut(s) | 745, 768, 1087 |
| BstX2I | RGATCY | 1 cut(s) | 233 |
| BstYI | RGATCY | 1 cut(s) | 233 |
| Bsu36I | CCTNAGG | 1 cut(s) | 711 |
| BsuRI | GGCC | 3 cut(s) | 204, 322, 464 |
| BtrI | CACGTC | 1 cut(s) | 140 |
| BtsI | GCAGTG | 1 cut(s) | 948 |
| BtsIMutI | CAGTG | 1 cut(s) | 948 |
| BveI | ACCTGC | 2 cut(s) | 173, 714 |
| Cac8I | GCNNGC | 2 cut(s) | 462, 1176 |
| CfoI | GCGC | 2 cut(s) | 327, 1011 |
| Cfr10I | RCCGGY | 1 cut(s) | 1066 |
| Cfr13I | GGNCC | 2 cut(s) | 203, 463 |
| Csp6I | GTAC | 4 cut(s) | 118, 160, 185, 1070 |
| CspAI | ACCGGT | 1 cut(s) | 1066 |
| CviAII | CATG | 8 cut(s) | 182, 342, 354, 728, 792, 823, 845, 995 |
| CviQI | GTAC | 4 cut(s) | 118, 160, 185, 1070 |
| DdeI | CTNAG | 2 cut(s) | 711, 869 |
| DpnI | GATC | 4 cut(s) | 153, 180, 235, 258 |
| DpnII | GATC | 4 cut(s) | 151, 178, 233, 256 |
| Eco57I | CTGAAG | 1 cut(s) | 406 |
| Eco81I | CCTNAGG | 1 cut(s) | 711 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 710 |
| FaeI | CATG | 8 cut(s) | 185, 345, 357, 731, 795, 826, 848, 998 |
| FatI | CATG | 8 cut(s) | 181, 341, 353, 727, 791, 822, 844, 994 |
| FbaI | TGATCA | 2 cut(s) | 151, 178 |
| FblI | GTMKAC | 1 cut(s) | 796 |
| Fnu4HI | GCNGC | 4 cut(s) | 323, 759, 782, 1101 |
| Fsp4HI | GCNGC | 4 cut(s) | 323, 759, 782, 1101 |
| FspBI | CTAG | 5 cut(s) | 762, 773, 887, 1121, 1190 |
| GlaI | GCGC | 2 cut(s) | 326, 1010 |
| GluI | GCNGC | 4 cut(s) | 323, 759, 782, 1101 |
| GsuI | CTGGAG | 1 cut(s) | 988 |
| HaeIII | GGCC | 3 cut(s) | 204, 322, 464 |
| HapII | CCGG | 2 cut(s) | 1067, 1165 |
| HhaI | GCGC | 2 cut(s) | 327, 1011 |
| Hin1II | CATG | 8 cut(s) | 185, 345, 357, 731, 795, 826, 848, 998 |
| Hin6I | GCGC | 2 cut(s) | 325, 1009 |
| HinP1I | GCGC | 2 cut(s) | 325, 1009 |
| HincII | GTYRAC | 1 cut(s) | 516 |
| HindII | GTYRAC | 1 cut(s) | 516 |
| HinfI | GANTC | 7 cut(s) | 21, 265, 367, 427, 625, 721, 1186 |
| HpaII | CCGG | 2 cut(s) | 1067, 1165 |
| HphI | GGTGA | 4 cut(s) | 263, 973, 1056, 1173 |
| Hpy166II | GTNNAC | 6 cut(s) | 10, 162, 516, 797, 946, 1064 |
| Hpy188I | TCNGA | 4 cut(s) | 542, 720, 870, 1046 |
| Hpy188III | TCNNGA | 7 cut(s) | 279, 359, 424, 550, 812, 887, 1190 |
| Hpy8I | GTNNAC | 6 cut(s) | 10, 162, 516, 797, 946, 1064 |
| Hpy99I | CGWCG | 1 cut(s) | 672 |
| HpyAV | CCTTC | 5 cut(s) | 70, 348, 414, 587, 875 |
| HpyCH4III | ACNGT | 3 cut(s) | 530, 536, 1087 |
| HpyCH4IV | ACGT | 1 cut(s) | 139 |
| HpyCH4V | TGCA | 3 cut(s) | 168, 433, 560 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 210, 457, 778 |
| HpyF3I | CTNAG | 2 cut(s) | 711, 869 |
| HpySE526I | ACGT | 1 cut(s) | 139 |
| Hsp92II | CATG | 8 cut(s) | 185, 345, 357, 731, 795, 826, 848, 998 |
| HspAI | GCGC | 2 cut(s) | 325, 1009 |
| Ksp22I | TGATCA | 2 cut(s) | 151, 178 |
| Kzo9I | GATC | 4 cut(s) | 151, 178, 233, 256 |
| LmnI | GCTCC | 4 cut(s) | 841, 881, 938, 1167 |
| Lsp1109I | GCAGC | 3 cut(s) | 745, 768, 1087 |
| LweI | GCATC | 3 cut(s) | 324, 547, 1087 |
| MaeI | CTAG | 5 cut(s) | 762, 773, 887, 1121, 1190 |
| MaeII | ACGT | 1 cut(s) | 139 |
| MaeIII | GTNAC | 4 cut(s) | 194, 454, 530, 979 |
| MalI | GATC | 4 cut(s) | 153, 180, 235, 258 |
| MboI | GATC | 4 cut(s) | 151, 178, 233, 256 |
| MboII | GAAGA | 2 cut(s) | 260, 560 |
| MflI | RGATCY | 1 cut(s) | 233 |
| MlyI | GAGTC | 3 cut(s) | 376, 436, 730 |
| MmeI | TCCRAC | 1 cut(s) | 456 |
| MnlI | CCTC | 7 cut(s) | 34, 81, 108, 504, 510, 720, 1189 |
| MseI | TTAA | 5 cut(s) | 191, 306, 447, 732, 1152 |
| MspCI | CTTAAG | 1 cut(s) | 190 |
| MspI | CCGG | 2 cut(s) | 1067, 1165 |
| Mva1269I | GAATGC | 2 cut(s) | 410, 642 |
| MvnI | CGCG | 1 cut(s) | 325 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 210, 457, 778 |
| NdeII | GATC | 4 cut(s) | 151, 178, 233, 256 |
| NlaIII | CATG | 8 cut(s) | 185, 345, 357, 731, 795, 826, 848, 998 |
| NlaIV | GGNNCC | 1 cut(s) | 235 |
| NmuCI | GTSAC | 3 cut(s) | 194, 530, 979 |
| NspI | RCATGY | 3 cut(s) | 826, 848, 998 |
| PaqCI | CACCTGC | 1 cut(s) | 173 |
| PciI | ACATGT | 2 cut(s) | 822, 994 |
| PctI | GAATGC | 2 cut(s) | 410, 642 |
| PfeI | GAWTC | 4 cut(s) | 21, 265, 625, 1186 |
| PinAI | ACCGGT | 1 cut(s) | 1066 |
| PkrI | GCNGC | 4 cut(s) | 324, 760, 783, 1102 |
| PleI | GAGTC | 3 cut(s) | 375, 435, 729 |
| PpsI | GAGTC | 3 cut(s) | 375, 435, 729 |
| PscI | ACATGT | 2 cut(s) | 822, 994 |
| PsiI | TTATAA | 1 cut(s) | 1058 |
| PspN4I | GGNNCC | 1 cut(s) | 235 |
| PspPI | GGNCC | 2 cut(s) | 203, 463 |
| PsuI | RGATCY | 1 cut(s) | 233 |
| RsaI | GTAC | 4 cut(s) | 119, 161, 186, 1071 |
| RsaNI | GTAC | 4 cut(s) | 118, 160, 185, 1070 |
| SaqAI | TTAA | 5 cut(s) | 191, 306, 447, 732, 1152 |
| SatI | GCNGC | 4 cut(s) | 323, 759, 782, 1101 |
| Sau3AI | GATC | 4 cut(s) | 151, 178, 233, 256 |
| Sau96I | GGNCC | 2 cut(s) | 203, 463 |
| SchI | GAGTC | 3 cut(s) | 376, 436, 730 |
| SfaNI | GCATC | 3 cut(s) | 324, 547, 1087 |
| SmlI | CTYRAG | 5 cut(s) | 190, 228, 506, 548, 1073 |
| SmoI | CTYRAG | 5 cut(s) | 190, 228, 506, 548, 1073 |
| SsiI | CCGC | 1 cut(s) | 323 |
| SspI | AATATT | 1 cut(s) | 57 |
| SspMI | CTAG | 5 cut(s) | 762, 773, 887, 1121, 1190 |
| TaaI | ACNGT | 3 cut(s) | 530, 536, 1087 |
| TaiI | ACGT | 1 cut(s) | 142 |
| TatI | WGTACW | 2 cut(s) | 159, 184 |
| TauI | GCSGC | 1 cut(s) | 325 |
| TfiI | GAWTC | 4 cut(s) | 21, 265, 625, 1186 |
| Tru1I | TTAA | 5 cut(s) | 191, 306, 447, 732, 1152 |
| Tru9I | TTAA | 5 cut(s) | 191, 306, 447, 732, 1152 |
| TscAI | CASTG | 1 cut(s) | 948 |
| TseFI | GTSAC | 3 cut(s) | 194, 530, 979 |
| TseI | GCWGC | 3 cut(s) | 758, 781, 1100 |
| Tsp45I | GTSAC | 3 cut(s) | 194, 530, 979 |
| TspDTI | ATGAA | 3 cut(s) | 330, 342, 1122 |
| TspRI | CASTG | 1 cut(s) | 948 |
| Vha464I | CTTAAG | 1 cut(s) | 190 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 710 |
| XapI | RAATTY | 4 cut(s) | 122, 308, 376, 1135 |
| XbaI | TCTAGA | 2 cut(s) | 886, 1189 |
| XceI | RCATGY | 3 cut(s) | 826, 848, 998 |
| XmiI | GTMKAC | 1 cut(s) | 796 |
| XspI | CTAG | 5 cut(s) | 762, 773, 887, 1121, 1190 |