Rh6CG059700

3-oxo-Delta(4,5)-steroid 5-beta-reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000626
Physical Location & Seq
Reverse (-)
5926163 .. 5929038
2876 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG059700.1

Sequence Viewer

Length: 1164 bp
ATGAGTTGGTGGTGGGCAGGAGCCATAGGAGCTGCAAAGAAAAAGTTTGATCCAGACGATGCACCAACCGAGTACCAGAGCGTGGCCCTCGTACTTGGGGTCACCGGAATCGTCGGAAACAGCCTCGCCGAGATTCTCCCACTTCCCGATACTCCCGGCGGGCCGTGGAAGGTCTATGGCGTGGCCCGGCGGCCCCGGCCGCAGTGGAACGTTGACCACCCGATTCAGTACATCCAGTGTGACCTCTCTGACTCTTCACAGACCGAGGCAGAGCTCTCCCAGCTCACTGATGTCACTCACATTTTTTATGTGACATGGGCGAGCAAGCCAACTGAAGCTGAGAACTGTGAGGCTAATGGGAAGATGTTGAGCAACGTGTTGAATACTGTGATCCCAAACGCGCCGAATTTGCAGCACATATGCATGCAGACGGGTCGGAAGCACTACTTAGGTTCGTATGAGATGTTGGGGAAAATCCAATCCCATGAACCTCCATATCACGAAGACCTGCCGAGGCTTAATTCCCCCAATTTTTACTATGTGATGGAGGATATTCTGTTTGAGGAGGTGAAGAAGAAGGAGGGGCTGACATGGTCTGTGCACAGGCCGGGAGTCATATTCGGGTTCTCACCTTATAGCATGATGAATGCGATTGGGAGTTTGTGTGTTTACGCTGCGATTTGTAAGCATGAAGGGCAGCCGTTGAGGTTTCCGGGGAGTAAAGGAACTTGGGAAGGGTTCTGGGACGTGTCTGATGCGGATATGATTGCTGAACATGAGATATGGGCAGCTGTGGAGCCTTTTGCCAAGAATGAAGCTTTTAATTGCAGCAATGGGGACGTGTTCAAGTGGAAGCAGTTGTGGGGGCTTTTGGCTGAGCAGTTCGAGGTAGTGCCTGCTGGAGTTCACGAGGAGTTGAGTTTTGAGGAGATGATGAAAGATAAAGGGCCGGTGTGGGATGAAATTGTGAGAGAGCATGGTTTGGTGCCTACCAAATTGGAAGAGGTTGGGAACTGGTGGTATCTGGATATATTGTTTCAGGTTGATTCAGCTGTGGACAGTATGAACAAGAGCAAAGAGCATGGTTTTGTTGGCTTTAGGAACTCGAAAACTTCCTTTGTTTCTTGGATTGACAAGATGAAGTCTTTTAGAATTATTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

387

Amino Acids

43.85

Weight (kDa)

5.16

Isoelectric Point (pI)

36.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 29 - 252 1.9e-07 NAD dependent epimerase/dehydratase family
PRISE PF22917 84 - 387 6.6e-25 PRISE-like Rossmann-fold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000626)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24220 AT4G24220
fragaria_vesca FvH4_2g04580 FvH4_2g04580 FvH4_2g04580 FvH4_4g06071 FvH4_5g25130
malus_domestica MD05G1094500.v1.1 MD05G1108300.v1.1 MD08G1088400.v1.1 MD15G1073200.v1.1 MD15G1073300.v1.1 MD15G1073400.v1.1 MD15G1073500.v1.1 MD15G1073600.v1.1
prunus_persica Prupe.1G426100_v2.0.a1 Prupe.1G426100_v2.0.a1 Prupe.1G426200_v2.0.a1 Prupe.1G426300_v2.0.a1 Prupe.1G426400_v2.0.a1 Prupe.1G426400_v2.0.a1 Prupe.8G139400_v2.0.a1 Prupe.8G139400_v2.0.a1
pyrus_communis pycom05g09350 pycom08g07190 pycom13g22780 pycom15g06840 pycom15g06850 pycom15g06870
rosa_chinensis RchiOBHm_Chr6g0254161 RchiOBHm_Chr6g0254181 RchiOBHm_Chr6g0254191 RchiOBHm_Chr6g0309171 RchiOBHm_Chr6g0309181 RchiOBHm_Chr6g0309191 RchiOBHm_Chr6g0309201
rosa_laevigata RLG00000010582 RLG00000010583 RLG00000014937 RLG00000014938
rosa_multiflora Rmu_co8060540.1_g000001 Rmu_co8332663.1_g000001 Rmu_sc0000010.1_g000017 Rmu_sc0003711.1_g000008 Rmu_sc0016971.1_g000003 Rmu_sc0020102.1_g000006 Rmu_sc0020102.1_g000008
rosa_roxburghii Rroxscaffold_7G00159570 Rroxscaffold_7G00159580 Rroxscaffold_7G00159590 Rroxscaffold_7G00211100
rosa_rugosa Rorug05G0550400 Rorug06G0370100 Rorug06G0370300
rosa_samantha Rh6AG066900 Rh6AG483200 Rh6AG483300 Rh6AG483400 Rh6BG059800 Rh6BG060000 Rh6BG492200 Rh6BG492300 Rh6BG492500 Rh6CG059700 Rh6CG059800 Rh6CG496900 Rh6CG497000 Rh6CG497200 Rh6CG497400 Rh6DG056700 Rh6DG056800 Rh6DG483800 Rh6DG484000
rosa_wichuraiana Rw6G005860 Rw6G005870 Rw6G042070 Rw6G042080 Rw6G042090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 516
AccB1I GGYRCC 1 cut(s) 985
AccB7I CCANNNNNTGG 1 cut(s) 82
AccII CGCG 1 cut(s) 401
AciI CCGC 4 cut(s) 159, 190, 200, 758
AclI AACGTT 1 cut(s) 210
AclWI GGATC 2 cut(s) 44, 385
AcoI YGGCCR 1 cut(s) 197
AcsI RAATTY 1 cut(s) 406
AcuI CTGAAG 1 cut(s) 354
AfaI GTAC 3 cut(s) 74, 93, 230
AfiI CCNNNNNNNGG 1 cut(s) 82
AflIII ACRYGT 3 cut(s) 375, 747, 840
AgsI TTSAA 2 cut(s) 382, 847
AjiI CACGTC 2 cut(s) 748, 841
AluBI AGCT 7 cut(s) 32, 274, 283, 338, 791, 818, 1052
AluI AGCT 7 cut(s) 32, 274, 283, 338, 791, 818, 1052
Alw21I GWGCWC 2 cut(s) 276, 603
Alw44I GTGCAC 1 cut(s) 599
AlwI GGATC 2 cut(s) 44, 385
AoxI GGCC 7 cut(s) 84, 161, 183, 191, 197, 605, 947
ApaLI GTGCAC 1 cut(s) 599
ApeKI GCWGC 6 cut(s) 32, 412, 674, 697, 788, 828
ApoI RAATTY 1 cut(s) 406
AspLEI GCGC 1 cut(s) 403
AspS9I GGNCC 5 cut(s) 85, 161, 184, 192, 947
AsuC2I CCSGG 5 cut(s) 156, 187, 196, 609, 714
AsuHPI GGTGA 3 cut(s) 94, 580, 621
BaeGI GKGCMC 1 cut(s) 603
BanI GGYRCC 1 cut(s) 985
BanII GRGCYC 1 cut(s) 276
BauI CACGAG 1 cut(s) 908
BbsI GAAGAC 1 cut(s) 510
Bbv12I GWGCWC 2 cut(s) 276, 603
BbvI GCAGC 6 cut(s) 19, 424, 661, 709, 800, 840
BccI CCATC 1 cut(s) 538
BceAI ACGGC 2 cut(s) 148, 685
BcgI CGANNNNNNTGC 2 cut(s) 416, 450
BcnI CCSGG 5 cut(s) 156, 187, 196, 609, 714
BfuAI ACCTGC 1 cut(s) 516
BisI GCNGC 8 cut(s) 33, 191, 200, 413, 675, 698, 789, 829
BlpI GCTNAGC 1 cut(s) 876
BlsI GCNGC 8 cut(s) 34, 192, 201, 414, 676, 699, 790, 830
Bme1390I CCNGG 5 cut(s) 156, 187, 196, 609, 714
BmgBI CACGTC 2 cut(s) 748, 841
BmgT120I GGNCC 5 cut(s) 85, 161, 184, 192, 947
BmiI GGNNCC 4 cut(s) 22, 194, 798, 987
BmrFI CCNGG 5 cut(s) 156, 187, 196, 609, 714
BmsI GCATC 2 cut(s) 49, 745
BpiI GAAGAC 1 cut(s) 510
BpmI CTGGAG 1 cut(s) 921
Bpu1102I GCTNAGC 1 cut(s) 876
BpuMI CCSGG 5 cut(s) 156, 187, 196, 609, 714
BsaJI CCNNGG 5 cut(s) 164, 194, 264, 512, 713
BsaWI WCCGGW 1 cut(s) 104
BsaXI ACNNNNNCTCC 2 cut(s) 649, 679
Bsc4I CCNNNNNNNGG 1 cut(s) 82
Bse118I RCCGGY 1 cut(s) 949
Bse1I ACTGG 2 cut(s) 235, 1019
Bse3DI GCAATG 1 cut(s) 838
BseDI CCNNGG 5 cut(s) 164, 194, 264, 512, 713
BseGI GGATG 2 cut(s) 231, 964
BseLI CCNNNNNNNGG 1 cut(s) 82
BseMI GCAATG 1 cut(s) 838
BseMII CTCAG 2 cut(s) 330, 867
BseNI ACTGG 2 cut(s) 235, 1019
BseRI GAGGAG 3 cut(s) 578, 926, 941
BseSI GKGCMC 1 cut(s) 603
BseX3I CGGCCG 1 cut(s) 197
BseXI GCAGC 6 cut(s) 19, 424, 661, 709, 800, 840
BseYI CCCAGC 1 cut(s) 279
Bsh1236I CGCG 1 cut(s) 401
Bsh1285I CGRYCG 1 cut(s) 200
BshFI GGCC 7 cut(s) 86, 163, 185, 193, 199, 607, 949
BshNI GGYRCC 1 cut(s) 985
BsiEI CGRYCG 1 cut(s) 200
BsiHKAI GWGCWC 2 cut(s) 276, 603
BsiSI CCGG 7 cut(s) 105, 156, 187, 196, 608, 713, 950
BslFI GGGAC 2 cut(s) 758, 851
BslI CCNNNNNNNGG 1 cut(s) 82
BsmFI GGGAC 2 cut(s) 758, 851
BsmI GAATGC 1 cut(s) 652
BsnI GGCC 7 cut(s) 86, 163, 185, 193, 199, 607, 949
Bsp1286I GDGCHC 2 cut(s) 276, 603
Bsp143I GATC 2 cut(s) 49, 390
Bsp1720I GCTNAGC 1 cut(s) 876
BspACI CCGC 4 cut(s) 159, 190, 200, 758
BspANI GGCC 7 cut(s) 86, 163, 185, 193, 199, 607, 949
BspCNI CTCAG 2 cut(s) 331, 868
BspFNI CGCG 1 cut(s) 401
BspLI GGNNCC 4 cut(s) 22, 194, 798, 987
BspMI ACCTGC 1 cut(s) 516
BspPI GGATC 2 cut(s) 44, 385
BspT107I GGYRCC 1 cut(s) 985
BsrDI GCAATG 1 cut(s) 838
BsrFI RCCGGY 1 cut(s) 949
BsrI ACTGG 2 cut(s) 235, 1019
BssAI RCCGGY 1 cut(s) 949
BssECI CCNNGG 5 cut(s) 164, 194, 264, 512, 713
BssMI GATC 2 cut(s) 49, 390
BssSI CACGAG 1 cut(s) 908
Bst2BI CACGAG 1 cut(s) 908
Bst4CI ACNGT 3 cut(s) 347, 388, 1061
Bst6I CTCTTC 2 cut(s) 259, 996
BstC8I GCNNGC 5 cut(s) 161, 322, 326, 425, 897
BstDEI CTNAG 3 cut(s) 339, 448, 876
BstDSI CCRYGG 1 cut(s) 164
BstEII GGTNACC 1 cut(s) 100
BstF5I GGATG 2 cut(s) 231, 964
BstFNI CGCG 1 cut(s) 401
BstHHI GCGC 1 cut(s) 403
BstKTI GATC 2 cut(s) 52, 393
BstMBI GATC 2 cut(s) 49, 390
BstMCI CGRYCG 1 cut(s) 200
BstMWI GCNNNNNNNGC 6 cut(s) 29, 196, 199, 280, 409, 694
BstNSI RCATGY 1 cut(s) 427
BstPI GGTNACC 1 cut(s) 100
BstSCI CCNGG 5 cut(s) 154, 185, 194, 607, 712
BstSLI GKGCMC 1 cut(s) 603
BstUI CGCG 1 cut(s) 401
BstV1I GCAGC 6 cut(s) 19, 424, 661, 709, 800, 840
BstV2I GAAGAC 1 cut(s) 510
BstZI CGGCCG 1 cut(s) 197
BsuRI GGCC 7 cut(s) 86, 163, 185, 193, 199, 607, 949
BtgI CCRYGG 1 cut(s) 164
BtrI CACGTC 2 cut(s) 748, 841
BtsCI GGATG 2 cut(s) 231, 964
BtsI GCAGTG 1 cut(s) 209
BtsIMutI CAGTG 3 cut(s) 209, 242, 285
BveI ACCTGC 1 cut(s) 516
Cac8I GCNNGC 5 cut(s) 161, 322, 326, 425, 897
CfoI GCGC 1 cut(s) 403
Cfr10I RCCGGY 1 cut(s) 949
Cfr13I GGNCC 5 cut(s) 85, 161, 184, 192, 947
Csp6I GTAC 3 cut(s) 73, 92, 229
CviAII CATG 9 cut(s) 315, 424, 485, 591, 640, 689, 776, 977, 1082
CviQI GTAC 3 cut(s) 73, 92, 229
DdeI CTNAG 3 cut(s) 339, 448, 876
DpnI GATC 2 cut(s) 51, 392
DpnII GATC 2 cut(s) 49, 390
EaeI YGGCCR 1 cut(s) 197
EagI CGGCCG 1 cut(s) 197
Eam1104I CTCTTC 2 cut(s) 259, 996
EarI CTCTTC 2 cut(s) 259, 996
Ecl136II GAGCTC 1 cut(s) 274
EclXI CGGCCG 1 cut(s) 197
Eco24I GRGCYC 1 cut(s) 276
Eco52I CGGCCG 1 cut(s) 197
Eco53kI GAGCTC 1 cut(s) 274
Eco57I CTGAAG 1 cut(s) 354
Eco91I GGTNACC 1 cut(s) 100
EcoICRI GAGCTC 1 cut(s) 274
EcoO65I GGTNACC 1 cut(s) 100
EcoT22I ATGCAT 1 cut(s) 425
EcoT38I GRGCYC 1 cut(s) 276
FaeI CATG 9 cut(s) 318, 427, 488, 594, 643, 692, 779, 980, 1085
FalI AAGNNNNNCTT 2 cut(s) 431, 463
FaqI GGGAC 2 cut(s) 758, 851
FatI CATG 9 cut(s) 314, 423, 484, 590, 639, 688, 775, 976, 1081
FauI CCCGC 1 cut(s) 152
FauNDI CATATG 1 cut(s) 419
Fnu4HI GCNGC 8 cut(s) 33, 191, 200, 413, 675, 698, 789, 829
FokI GGATG 2 cut(s) 218, 971
FriOI GRGCYC 1 cut(s) 276
Fsp4HI GCNGC 8 cut(s) 33, 191, 200, 413, 675, 698, 789, 829
GlaI GCGC 1 cut(s) 402
GluI GCNGC 8 cut(s) 33, 191, 200, 413, 675, 698, 789, 829
GsaI CCCAGC 1 cut(s) 283
GsuI CTGGAG 1 cut(s) 921
HaeIII GGCC 7 cut(s) 86, 163, 185, 193, 199, 607, 949
HapII CCGG 7 cut(s) 105, 156, 187, 196, 608, 713, 950
HhaI GCGC 1 cut(s) 403
Hin1II CATG 9 cut(s) 318, 427, 488, 594, 643, 692, 779, 980, 1085
Hin6I GCGC 1 cut(s) 401
HinP1I GCGC 1 cut(s) 401
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HindIII AAGCTT 1 cut(s) 816
HinfI GANTC 6 cut(s) 108, 133, 223, 251, 612, 1046
HpaII CCGG 7 cut(s) 105, 156, 187, 196, 608, 713, 950
HphI GGTGA 3 cut(s) 94, 580, 621
Hpy166II GTNNAC 5 cut(s) 214, 601, 670, 907, 1057
Hpy188I TCNGA 4 cut(s) 116, 250, 438, 754
Hpy188III TCNNGA 5 cut(s) 53, 146, 500, 908, 1025
Hpy8I GTNNAC 5 cut(s) 214, 601, 670, 907, 1057
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 4 cut(s) 163, 571, 686, 728
HpyCH4III ACNGT 3 cut(s) 347, 388, 1061
HpyCH4IV ACGT 4 cut(s) 210, 375, 747, 840
HpyCH4V TGCA 7 cut(s) 35, 62, 412, 423, 427, 601, 828
HpyF10VI GCNNNNNNNGC 6 cut(s) 29, 196, 199, 280, 409, 694
HpyF3I CTNAG 3 cut(s) 339, 448, 876
HpySE526I ACGT 4 cut(s) 210, 375, 747, 840
Hsp92II CATG 9 cut(s) 318, 427, 488, 594, 643, 692, 779, 980, 1085
HspAI GCGC 1 cut(s) 401
Kzo9I GATC 2 cut(s) 49, 390
LmnI GCTCC 3 cut(s) 20, 29, 796
Lsp1109I GCAGC 6 cut(s) 19, 424, 661, 709, 800, 840
LweI GCATC 2 cut(s) 49, 745
MaeII ACGT 4 cut(s) 210, 375, 747, 840
MaeIII GTNAC 4 cut(s) 100, 239, 292, 310
MalI GATC 2 cut(s) 51, 392
MboI GATC 2 cut(s) 49, 390
MboII GAAGA 6 cut(s) 246, 373, 515, 583, 586, 1013
MhlI GDGCHC 2 cut(s) 276, 603
MluCI AATT 7 cut(s) 406, 520, 529, 823, 963, 995, 1152
MlyI GAGTC 2 cut(s) 245, 621
MmeI TCCRAC 2 cut(s) 94, 416
Mph1103I ATGCAT 1 cut(s) 425
MseI TTAA 2 cut(s) 519, 822
MslI CAYNNNNRTG 1 cut(s) 422
MspA1I CMGCKG 2 cut(s) 791, 1052
MspI CCGG 7 cut(s) 105, 156, 187, 196, 608, 713, 950
MspR9I CCNGG 5 cut(s) 156, 187, 196, 609, 714
Mva1269I GAATGC 1 cut(s) 652
MvnI CGCG 1 cut(s) 401
MwoI GCNNNNNNNGC 6 cut(s) 29, 196, 199, 280, 409, 694
NciI CCSGG 5 cut(s) 156, 187, 196, 609, 714
NdeI CATATG 1 cut(s) 419
NdeII GATC 2 cut(s) 49, 390
NlaIII CATG 9 cut(s) 318, 427, 488, 594, 643, 692, 779, 980, 1085
NlaIV GGNNCC 4 cut(s) 22, 194, 798, 987
NmeAIII GCCGAG 2 cut(s) 154, 537
NmuCI GTSAC 4 cut(s) 100, 239, 292, 310
NsiI ATGCAT 1 cut(s) 425
NspI RCATGY 1 cut(s) 427
PaeI GCATGC 1 cut(s) 427
PctI GAATGC 1 cut(s) 652
PfeI GAWTC 4 cut(s) 108, 133, 223, 1046
PflFI GACNNNGTC 1 cut(s) 592
PflMI CCANNNNNTGG 1 cut(s) 82
PkrI GCNGC 8 cut(s) 34, 192, 201, 414, 676, 699, 790, 830
PleI GAGTC 2 cut(s) 245, 620
PpsI GAGTC 2 cut(s) 245, 620
Psp124BI GAGCTC 1 cut(s) 276
Psp1406I AACGTT 1 cut(s) 210
PspEI GGTNACC 1 cut(s) 100
PspFI CCCAGC 1 cut(s) 279
PspN4I GGNNCC 4 cut(s) 22, 194, 798, 987
PspPI GGNCC 5 cut(s) 85, 161, 184, 192, 947
PsyI GACNNNGTC 1 cut(s) 592
PvuII CAGCTG 2 cut(s) 791, 1052
RsaI GTAC 3 cut(s) 74, 93, 230
RsaNI GTAC 3 cut(s) 73, 92, 229
RseI CAYNNNNRTG 1 cut(s) 422
SacI GAGCTC 1 cut(s) 276
SaqAI TTAA 2 cut(s) 519, 822
SatI GCNGC 8 cut(s) 33, 191, 200, 413, 675, 698, 789, 829
Sau3AI GATC 2 cut(s) 49, 390
Sau96I GGNCC 5 cut(s) 85, 161, 184, 192, 947
SchI GAGTC 2 cut(s) 245, 621
ScrFI CCNGG 5 cut(s) 156, 187, 196, 609, 714
SduI GDGCHC 2 cut(s) 276, 603
SfaNI GCATC 2 cut(s) 49, 745
SmiMI CAYNNNNRTG 1 cut(s) 422
SphI GCATGC 1 cut(s) 427
Sse9I AATT 7 cut(s) 406, 520, 529, 823, 963, 995, 1152
SsiI CCGC 4 cut(s) 159, 190, 200, 758
SstI GAGCTC 1 cut(s) 276
StyD4I CCNGG 5 cut(s) 154, 185, 194, 607, 712
TaaI ACNGT 3 cut(s) 347, 388, 1061
TaiI ACGT 4 cut(s) 213, 378, 750, 843
TaqI TCGA 2 cut(s) 885, 1106
TaqII GACCGA 1 cut(s) 278
TasI AATT 7 cut(s) 406, 520, 529, 823, 963, 995, 1152
TatI WGTACW 1 cut(s) 228
TauI GCSGC 2 cut(s) 193, 202
TfiI GAWTC 4 cut(s) 108, 133, 223, 1046
Tru1I TTAA 2 cut(s) 519, 822
Tru9I TTAA 2 cut(s) 519, 822
TscAI CASTG 3 cut(s) 209, 242, 292
TseFI GTSAC 4 cut(s) 100, 239, 292, 310
TseI GCWGC 6 cut(s) 32, 412, 674, 697, 788, 828
Tsp45I GTSAC 4 cut(s) 100, 239, 292, 310
TspDTI ATGAA 8 cut(s) 501, 659, 705, 828, 950, 975, 1079, 1154
TspRI CASTG 3 cut(s) 209, 242, 292
Tth111I GACNNNGTC 1 cut(s) 592
Van91I CCANNNNNTGG 1 cut(s) 82
VneI GTGCAC 1 cut(s) 599
XapI RAATTY 1 cut(s) 406
XceI RCATGY 1 cut(s) 427
Zsp2I ATGCAT 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.