Prupe.8G139400_v2.0.a1

3-oxo-Delta(4,5)-steroid 5-beta-reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000626
Physical Location & Seq
Forward (+)
15825871 .. 15829371
3501 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G139400.2

Sequence Viewer

Length: 1179 bp
ATGAGCTGGTGGTGGGCAGGAGCCATAGGAGCTGCAAAGAAAAAGTTTGATCCCAACGATGCACCACCCAAGTACCAGAGCGTGGCACTGGTGGCTGGGGTCACGGGGATCGTGGGCAACAGCCTTGCTGATATCCTGCCGCTCCCCGACACCCCCGGTGGGCCCTGGAAGGTCTACGGGGTAGCCCGCAGGGCCCGCCCGGATTGGAACGCTGATCATCCCATCGAGTACATCCAATGTGATCTCTCAGACCCTCATCAAACACATTCTGAACTCTCCCAACTGACCGATGTCACACACATCTTTTACGTCACTTGGGCCAGCAAGCCCACGGAAGCAGAGAACTGCGAGGCCAATGGCAACATGTTTCGAAACTTGTTGGATGCTGTGATCCCAAATGCACCAAATTTGCAGCACATATGCTTGCAAACAGGTCGCAAGCATTACGTTGGTTCATTTGAGATGTTGGGAAAAGTTGAGCCTCATGAGCCTCCATTTCATGAGGACCTCCCGAGGCTCAACGTGCCCAACTTTTACTACACATTGGAGGACATTTTGTTTGAGGAGGTGAAGAAGGAGGGGTTGACATGGTCAGTGCACAGGCCTACTGTGATTTTCGGGTTTTCGCCGTATTGCTTGATGAACATAGTGGGGAGTTTGTGTGTTTACGCTGCGATATGTAAGCACGTAGGGAAGAAACTGAGGTTTTCGGGGAGCAGAGGAGTGTGGAACGGGTACTGGGATGCCTCAGATGCTGACCTGATTGCTGAGCATCAGATATGGGCAGCAGTTGATCCTTATGCAAAGAATGAGGCTTTCAATTGCAGCAATGGGGATGTGTATAAGTGGAAGCATTTGTGGAGGGTTTTGGCAGAACAGTTTGACATGGAGTTTGAGGACTTTGATGATGATGACGAGGGCGAATTGAGGCCGAGCATGGAGGAGATGATGAAAGATAAGGGGCCAGTGTGGGATGAGATTGTGAGAGAGAATGATCTGGTGCCTACAAAATTGGAAGAGATTGGGAATTGGTGGTTTGTGGATGTCATGTTTGGGGTAGAGTCAAGTGTGGACAGTATGAATAAGAGCAAAGAGCATGGCTTTGTGGGGTTCAGGAACTCAAGGACATCATTTGTAACTTGGATTGATAAGATGAAATCTTACAGACTTGTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

393

Amino Acids

44.74

Weight (kDa)

5.1

Isoelectric Point (pI)

33.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000626)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24220 AT4G24220
fragaria_vesca FvH4_2g04580 FvH4_2g04580 FvH4_2g04580 FvH4_4g06071 FvH4_5g25130
malus_domestica MD05G1094500.v1.1 MD05G1108300.v1.1 MD08G1088400.v1.1 MD15G1073200.v1.1 MD15G1073300.v1.1 MD15G1073400.v1.1 MD15G1073500.v1.1 MD15G1073600.v1.1
prunus_persica Prupe.1G426100_v2.0.a1 Prupe.1G426100_v2.0.a1 Prupe.1G426200_v2.0.a1 Prupe.1G426300_v2.0.a1 Prupe.1G426400_v2.0.a1 Prupe.1G426400_v2.0.a1 Prupe.8G139400_v2.0.a1 Prupe.8G139400_v2.0.a1
pyrus_communis pycom05g09350 pycom08g07190 pycom13g22780 pycom15g06840 pycom15g06850 pycom15g06870
rosa_chinensis RchiOBHm_Chr6g0254161 RchiOBHm_Chr6g0254181 RchiOBHm_Chr6g0254191 RchiOBHm_Chr6g0309171 RchiOBHm_Chr6g0309181 RchiOBHm_Chr6g0309191 RchiOBHm_Chr6g0309201
rosa_laevigata RLG00000010582 RLG00000010583 RLG00000014937 RLG00000014938
rosa_multiflora Rmu_co8060540.1_g000001 Rmu_co8332663.1_g000001 Rmu_sc0000010.1_g000017 Rmu_sc0003711.1_g000008 Rmu_sc0016971.1_g000003 Rmu_sc0020102.1_g000006 Rmu_sc0020102.1_g000008
rosa_roxburghii Rroxscaffold_7G00159570 Rroxscaffold_7G00159580 Rroxscaffold_7G00159590 Rroxscaffold_7G00211100
rosa_rugosa Rorug05G0550400 Rorug06G0370100 Rorug06G0370300
rosa_samantha Rh6AG066900 Rh6AG483200 Rh6AG483300 Rh6AG483400 Rh6BG059800 Rh6BG060000 Rh6BG492200 Rh6BG492300 Rh6BG492500 Rh6CG059700 Rh6CG059800 Rh6CG496900 Rh6CG497000 Rh6CG497200 Rh6CG497400 Rh6DG056700 Rh6DG056800 Rh6DG483800 Rh6DG484000
rosa_wichuraiana Rw6G005860 Rw6G005870 Rw6G042070 Rw6G042080 Rw6G042090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1000
AccB7I CCANNNNNTGG 1 cut(s) 82
AccBSI CCGCTC 1 cut(s) 142
AccI GTMKAC 1 cut(s) 174
AciI CCGC 3 cut(s) 140, 187, 196
AclWI GGATC 4 cut(s) 44, 116, 385, 788
AcsI RAATTY 1 cut(s) 406
AfaI GTAC 3 cut(s) 74, 230, 737
AfiI CCNNNNNNNGG 2 cut(s) 82, 159
AflIII ACRYGT 1 cut(s) 363
AgsI TTSAA 1 cut(s) 820
AjnI CCWGG 1 cut(s) 164
AluBI AGCT 2 cut(s) 6, 32
AluI AGCT 2 cut(s) 6, 32
Alw21I GWGCWC 1 cut(s) 600
Alw44I GTGCAC 1 cut(s) 596
AlwI GGATC 4 cut(s) 44, 116, 385, 788
AlwNI CAGNNNCTG 1 cut(s) 755
Ama87I CYCGRG 1 cut(s) 511
AoxI GGCC 7 cut(s) 161, 192, 318, 351, 602, 929, 962
ApaI GGGCCC 2 cut(s) 165, 196
ApaLI GTGCAC 1 cut(s) 596
ApeKI GCWGC 5 cut(s) 32, 412, 671, 785, 825
ApoI RAATTY 1 cut(s) 406
ArsI GACNNNNNNTTYG 4 cut(s) 542, 574, 875, 907
AspS9I GGNCC 7 cut(s) 161, 162, 192, 193, 318, 505, 962
AsuC2I CCSGG 2 cut(s) 156, 200
AsuHPI GGTGA 1 cut(s) 580
AsuII TTCGAA 1 cut(s) 370
AvaI CYCGRG 1 cut(s) 511
AvaII GGWCC 1 cut(s) 505
BaeGI GKGCMC 4 cut(s) 165, 196, 528, 600
BaeI ACNNNNGTAYC 2 cut(s) 56, 89
BanI GGYRCC 1 cut(s) 1000
BanII GRGCYC 2 cut(s) 165, 196
Bbv12I GWGCWC 1 cut(s) 600
BbvI GCAGC 5 cut(s) 19, 424, 658, 797, 837
BccI CCATC 1 cut(s) 230
BceAI ACGGC 1 cut(s) 613
BcgI CGANNNNNNTGC 2 cut(s) 416, 450
BciT130I CCWGG 1 cut(s) 166
BclI TGATCA 1 cut(s) 214
BcnI CCSGG 2 cut(s) 156, 200
BglI GCCNNNNNGGC 1 cut(s) 191
BisI GCNGC 6 cut(s) 33, 140, 413, 672, 786, 826
BlpI GCTNAGC 1 cut(s) 768
BlsI GCNGC 6 cut(s) 34, 141, 414, 673, 787, 827
Bme1390I CCNGG 3 cut(s) 156, 166, 200
Bme18I GGWCC 1 cut(s) 505
BmeT110I CYCGRG 1 cut(s) 511
BmgT120I GGNCC 7 cut(s) 161, 162, 192, 193, 318, 505, 962
BmiI GGNNCC 5 cut(s) 22, 163, 194, 963, 1002
BmrFI CCNGG 3 cut(s) 156, 166, 200
BmrI ACTGGG 1 cut(s) 748
BmsI GCATC 5 cut(s) 49, 373, 733, 742, 781
BmuI ACTGGG 1 cut(s) 748
BoxI GACNNNNGTC 1 cut(s) 290
Bpu1102I GCTNAGC 1 cut(s) 768
Bpu14I TTCGAA 1 cut(s) 370
BpuEI CTTGAG 1 cut(s) 1105
BpuMI CCSGG 2 cut(s) 156, 200
BsaAI YACGTR 1 cut(s) 688
BsaJI CCNNGG 4 cut(s) 154, 164, 330, 512
BsaXI ACNNNNNCTCC 2 cut(s) 646, 676
Bsc4I CCNNNNNNNGG 2 cut(s) 82, 159
Bse1I ACTGG 3 cut(s) 93, 743, 965
Bse3DI GCAATG 1 cut(s) 835
BseBI CCWGG 1 cut(s) 166
BseDI CCNNGG 4 cut(s) 154, 164, 330, 512
BseGI GGATG 7 cut(s) 217, 231, 388, 748, 841, 979, 1048
BseLI CCNNNNNNNGG 2 cut(s) 82, 159
BseMI GCAATG 1 cut(s) 835
BseMII CTCAG 4 cut(s) 261, 692, 759, 762
BseNI ACTGG 3 cut(s) 93, 743, 965
BseRI GAGGAG 3 cut(s) 578, 735, 956
BseSI GKGCMC 4 cut(s) 165, 196, 528, 600
BseXI GCAGC 5 cut(s) 19, 424, 658, 797, 837
BseYI CCCAGC 1 cut(s) 95
BshFI GGCC 7 cut(s) 163, 194, 320, 353, 604, 931, 964
BshNI GGYRCC 1 cut(s) 1000
BsiHKAI GWGCWC 1 cut(s) 600
BsiHKCI CYCGRG 1 cut(s) 511
BsiSI CCGG 2 cut(s) 156, 200
BslI CCNNNNNNNGG 2 cut(s) 82, 159
BsnI GGCC 7 cut(s) 163, 194, 320, 353, 604, 931, 964
BsoBI CYCGRG 1 cut(s) 511
Bsp119I TTCGAA 1 cut(s) 370
Bsp120I GGGCCC 2 cut(s) 161, 192
Bsp1286I GDGCHC 4 cut(s) 165, 196, 528, 600
Bsp143I GATC 7 cut(s) 49, 108, 214, 241, 390, 793, 994
Bsp1720I GCTNAGC 1 cut(s) 768
BspACI CCGC 3 cut(s) 140, 187, 196
BspANI GGCC 7 cut(s) 163, 194, 320, 353, 604, 931, 964
BspCNI CTCAG 4 cut(s) 260, 693, 760, 761
BspHI TCATGA 2 cut(s) 484, 499
BspLI GGNNCC 5 cut(s) 22, 163, 194, 963, 1002
BspPI GGATC 4 cut(s) 44, 116, 385, 788
BspT104I TTCGAA 1 cut(s) 370
BspT107I GGYRCC 1 cut(s) 1000
BsrBI CCGCTC 1 cut(s) 142
BsrDI GCAATG 1 cut(s) 835
BsrI ACTGG 3 cut(s) 93, 743, 965
BssECI CCNNGG 4 cut(s) 154, 164, 330, 512
BssMI GATC 7 cut(s) 49, 108, 214, 241, 390, 793, 994
Bst2UI CCWGG 1 cut(s) 166
Bst4CI ACNGT 3 cut(s) 610, 879, 1076
Bst6I CTCTTC 1 cut(s) 1011
BstBAI YACGTR 1 cut(s) 688
BstBI TTCGAA 1 cut(s) 370
BstC8I GCNNGC 6 cut(s) 187, 196, 322, 326, 425, 440
BstDEI CTNAG 4 cut(s) 247, 701, 748, 768
BstDSI CCRYGG 1 cut(s) 330
BstF5I GGATG 7 cut(s) 217, 231, 388, 748, 841, 979, 1048
BstKTI GATC 7 cut(s) 52, 111, 217, 244, 393, 796, 997
BstMBI GATC 7 cut(s) 49, 108, 214, 241, 390, 793, 994
BstMWI GCNNNNNNNGC 7 cut(s) 29, 92, 191, 195, 487, 523, 752
BstNI CCWGG 1 cut(s) 166
BstNSI RCATGY 1 cut(s) 367
BstPAI GACNNNNGTC 1 cut(s) 290
BstSCI CCNGG 3 cut(s) 154, 164, 198
BstSLI GKGCMC 4 cut(s) 165, 196, 528, 600
BstV1I GCAGC 5 cut(s) 19, 424, 658, 797, 837
BsuRI GGCC 7 cut(s) 163, 194, 320, 353, 604, 931, 964
BtgI CCRYGG 1 cut(s) 330
BtsCI GGATG 7 cut(s) 217, 231, 388, 748, 841, 979, 1048
BtsIMutI CAGTG 3 cut(s) 86, 600, 972
Cac8I GCNNGC 6 cut(s) 187, 196, 322, 326, 425, 440
CaiI CAGNNNCTG 1 cut(s) 755
CciI TCATGA 2 cut(s) 484, 499
Cfr13I GGNCC 7 cut(s) 161, 162, 192, 193, 318, 505, 962
Csp6I GTAC 3 cut(s) 73, 229, 736
CviAII CATG 8 cut(s) 364, 485, 500, 588, 886, 937, 1048, 1097
CviQI GTAC 3 cut(s) 73, 229, 736
DdeI CTNAG 4 cut(s) 247, 701, 748, 768
DpnI GATC 7 cut(s) 51, 110, 216, 243, 392, 795, 996
DpnII GATC 7 cut(s) 49, 108, 214, 241, 390, 793, 994
Eam1104I CTCTTC 1 cut(s) 1011
EarI CTCTTC 1 cut(s) 1011
Eco147I AGGCCT 1 cut(s) 604
Eco24I GRGCYC 2 cut(s) 165, 196
Eco32I GATATC 1 cut(s) 133
Eco47I GGWCC 1 cut(s) 505
Eco88I CYCGRG 1 cut(s) 511
EcoO109I RGGNCCY 3 cut(s) 162, 192, 505
EcoRII CCWGG 1 cut(s) 164
EcoRV GATATC 1 cut(s) 133
EcoT38I GRGCYC 2 cut(s) 165, 196
FaeI CATG 8 cut(s) 367, 488, 503, 591, 889, 940, 1051, 1100
FatI CATG 8 cut(s) 363, 484, 499, 587, 885, 936, 1047, 1096
FauI CCCGC 2 cut(s) 194, 203
FauNDI CATATG 1 cut(s) 419
FbaI TGATCA 1 cut(s) 214
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 6 cut(s) 33, 140, 413, 672, 786, 826
FokI GGATG 7 cut(s) 204, 218, 395, 755, 848, 986, 1055
FriOI GRGCYC 2 cut(s) 165, 196
Fsp4HI GCNGC 6 cut(s) 33, 140, 413, 672, 786, 826
GluI GCNGC 6 cut(s) 33, 140, 413, 672, 786, 826
GsaI CCCAGC 1 cut(s) 99
HaeIII GGCC 7 cut(s) 163, 194, 320, 353, 604, 931, 964
HapII CCGG 2 cut(s) 156, 200
Hin1II CATG 8 cut(s) 367, 488, 503, 591, 889, 940, 1051, 1100
HincII GTYRAC 1 cut(s) 585
HindII GTYRAC 1 cut(s) 585
HinfI GANTC 1 cut(s) 1061
HpaII CCGG 2 cut(s) 156, 200
HphI GGTGA 1 cut(s) 580
Hpy166II GTNNAC 5 cut(s) 175, 585, 598, 667, 1072
Hpy188I TCNGA 4 cut(s) 250, 271, 751, 777
Hpy188III TCNNGA 4 cut(s) 485, 500, 511, 1114
Hpy8I GTNNAC 5 cut(s) 175, 585, 598, 667, 1072
HpyAV CCTTC 2 cut(s) 163, 568
HpyCH4III ACNGT 3 cut(s) 610, 879, 1076
HpyCH4IV ACGT 4 cut(s) 309, 447, 522, 687
HpyCH4V TGCA 8 cut(s) 35, 62, 401, 412, 427, 598, 803, 825
HpyF10VI GCNNNNNNNGC 7 cut(s) 29, 92, 191, 195, 487, 523, 752
HpyF3I CTNAG 4 cut(s) 247, 701, 748, 768
HpySE526I ACGT 4 cut(s) 309, 447, 522, 687
Hsp92II CATG 8 cut(s) 367, 488, 503, 591, 889, 940, 1051, 1100
Ksp22I TGATCA 1 cut(s) 214
Kzo9I GATC 7 cut(s) 49, 108, 214, 241, 390, 793, 994
LmnI GCTCC 4 cut(s) 20, 29, 147, 714
Lsp1109I GCAGC 5 cut(s) 19, 424, 658, 797, 837
LweI GCATC 5 cut(s) 49, 373, 733, 742, 781
MaeII ACGT 4 cut(s) 309, 447, 522, 687
MaeIII GTNAC 4 cut(s) 100, 292, 310, 1135
MalI GATC 7 cut(s) 51, 110, 216, 243, 392, 795, 996
MbiI CCGCTC 1 cut(s) 142
MboI GATC 7 cut(s) 49, 108, 214, 241, 390, 793, 994
MboII GAAGA 3 cut(s) 583, 706, 1028
MfeI CAATTG 1 cut(s) 820
MhlI GDGCHC 4 cut(s) 165, 196, 528, 600
MluCI AATT 5 cut(s) 406, 820, 923, 1010, 1027
MlyI GAGTC 1 cut(s) 1070
MmeI TCCRAC 1 cut(s) 360
MspI CCGG 2 cut(s) 156, 200
MspR9I CCNGG 3 cut(s) 156, 166, 200
MunI CAATTG 1 cut(s) 820
MvaI CCWGG 1 cut(s) 166
MwoI GCNNNNNNNGC 7 cut(s) 29, 92, 191, 195, 487, 523, 752
NciI CCSGG 2 cut(s) 156, 200
NdeI CATATG 1 cut(s) 419
NdeII GATC 7 cut(s) 49, 108, 214, 241, 390, 793, 994
NlaIII CATG 8 cut(s) 367, 488, 503, 591, 889, 940, 1051, 1100
NlaIV GGNNCC 5 cut(s) 22, 163, 194, 963, 1002
NmeAIII GCCGAG 1 cut(s) 957
NmuCI GTSAC 3 cut(s) 100, 292, 310
NspI RCATGY 1 cut(s) 367
NspV TTCGAA 1 cut(s) 370
PagI TCATGA 2 cut(s) 484, 499
PceI AGGCCT 1 cut(s) 604
PciI ACATGT 1 cut(s) 363
PflFI GACNNNGTC 1 cut(s) 589
PflMI CCANNNNNTGG 1 cut(s) 82
PkrI GCNGC 6 cut(s) 34, 141, 414, 673, 787, 827
PleI GAGTC 1 cut(s) 1069
PpsI GAGTC 1 cut(s) 1069
Ppu21I YACGTR 1 cut(s) 688
PpuMI RGGWCCY 1 cut(s) 505
PscI ACATGT 1 cut(s) 363
PshAI GACNNNNGTC 1 cut(s) 290
Psp5II RGGWCCY 1 cut(s) 505
Psp6I CCWGG 1 cut(s) 164
PspFI CCCAGC 1 cut(s) 95
PspGI CCWGG 1 cut(s) 164
PspN4I GGNNCC 5 cut(s) 22, 163, 194, 963, 1002
PspOMI GGGCCC 2 cut(s) 161, 192
PspPI GGNCC 7 cut(s) 161, 162, 192, 193, 318, 505, 962
PspPPI RGGWCCY 1 cut(s) 505
PstNI CAGNNNCTG 1 cut(s) 755
PsyI GACNNNGTC 1 cut(s) 589
RsaI GTAC 3 cut(s) 74, 230, 737
RsaNI GTAC 3 cut(s) 73, 229, 736
SatI GCNGC 6 cut(s) 33, 140, 413, 672, 786, 826
Sau3AI GATC 7 cut(s) 49, 108, 214, 241, 390, 793, 994
Sau96I GGNCC 7 cut(s) 161, 162, 192, 193, 318, 505, 962
SchI GAGTC 1 cut(s) 1070
ScrFI CCNGG 3 cut(s) 156, 166, 200
SduI GDGCHC 4 cut(s) 165, 196, 528, 600
SfaNI GCATC 5 cut(s) 49, 373, 733, 742, 781
SfuI TTCGAA 1 cut(s) 370
SinI GGWCC 1 cut(s) 505
SmlI CTYRAG 1 cut(s) 1120
SmoI CTYRAG 1 cut(s) 1120
Sse9I AATT 5 cut(s) 406, 820, 923, 1010, 1027
SseBI AGGCCT 1 cut(s) 604
SsiI CCGC 3 cut(s) 140, 187, 196
StuI AGGCCT 1 cut(s) 604
StyD4I CCNGG 3 cut(s) 154, 164, 198
TaaI ACNGT 3 cut(s) 610, 879, 1076
TaiI ACGT 4 cut(s) 312, 450, 525, 690
TaqI TCGA 2 cut(s) 225, 370
TaqII GACCGA 1 cut(s) 302
TasI AATT 5 cut(s) 406, 820, 923, 1010, 1027
TatI WGTACW 1 cut(s) 228
TauI GCSGC 1 cut(s) 142
TscAI CASTG 3 cut(s) 93, 600, 972
TseFI GTSAC 3 cut(s) 100, 292, 310
TseI GCWGC 5 cut(s) 32, 412, 671, 785, 825
Tsp45I GTSAC 3 cut(s) 100, 292, 310
TspDTI ATGAA 6 cut(s) 444, 488, 656, 965, 1094, 1169
TspGWI ACGGA 1 cut(s) 347
TspRI CASTG 3 cut(s) 93, 600, 972
Tth111I GACNNNGTC 1 cut(s) 589
Van91I CCANNNNNTGG 1 cut(s) 82
VneI GTGCAC 1 cut(s) 596
VpaK11BI GGWCC 1 cut(s) 505
XapI RAATTY 1 cut(s) 406
XceI RCATGY 1 cut(s) 367
XmiI GTMKAC 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.