3-oxo-Delta(4,5)-steroid 5-beta-reductase-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 5 cut(s) | 83, 101, 152, 329, 360 |
| AclWI | GGATC | 3 cut(s) | 149, 162, 254 |
| AcsI | RAATTY | 2 cut(s) | 118, 421 |
| AgsI | TTSAA | 1 cut(s) | 64 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 419, 451 |
| AlwI | GGATC | 3 cut(s) | 149, 162, 254 |
| ApeKI | GCWGC | 2 cut(s) | 32, 211 |
| ApoI | RAATTY | 2 cut(s) | 118, 421 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 338, 370 |
| AspLEI | GCGC | 1 cut(s) | 19 |
| AsuC2I | CCSGG | 1 cut(s) | 72 |
| BamHI | GGATCC | 1 cut(s) | 154 |
| BbsI | GAAGAC | 1 cut(s) | 61 |
| BbvCI | CCTCAGC | 1 cut(s) | 287 |
| BbvI | GCAGC | 2 cut(s) | 19, 223 |
| BcnI | CCSGG | 1 cut(s) | 72 |
| BisI | GCNGC | 3 cut(s) | 33, 84, 212 |
| BlpI | GCTNAGC | 1 cut(s) | 234 |
| BlsI | GCNGC | 3 cut(s) | 34, 85, 213 |
| Bme1390I | CCNGG | 1 cut(s) | 72 |
| BmiI | GGNNCC | 1 cut(s) | 156 |
| BmrFI | CCNGG | 1 cut(s) | 72 |
| BmsI | GCATC | 1 cut(s) | 103 |
| BpiI | GAAGAC | 1 cut(s) | 61 |
| Bpu10I | CCTNAGC | 1 cut(s) | 287 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 234 |
| BpuMI | CCSGG | 1 cut(s) | 72 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 245, 275, 284, 314, 356, 386 |
| BseGI | GGATG | 1 cut(s) | 118 |
| BseMII | CTCAG | 2 cut(s) | 225, 278 |
| BseRI | GAGGAG | 3 cut(s) | 266, 287, 335 |
| BseXI | GCAGC | 2 cut(s) | 19, 223 |
| BsiSI | CCGG | 1 cut(s) | 72 |
| Bsp143I | GATC | 2 cut(s) | 154, 246 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 234 |
| BspACI | CCGC | 5 cut(s) | 83, 101, 152, 329, 360 |
| BspCNI | CTCAG | 2 cut(s) | 226, 279 |
| BspLI | GGNNCC | 1 cut(s) | 156 |
| BspPI | GGATC | 3 cut(s) | 149, 162, 254 |
| BssMI | GATC | 2 cut(s) | 154, 246 |
| BstDEI | CTNAG | 3 cut(s) | 234, 287, 312 |
| BstF5I | GGATG | 1 cut(s) | 118 |
| BstHHI | GCGC | 1 cut(s) | 19 |
| BstKTI | GATC | 2 cut(s) | 157, 249 |
| BstMBI | GATC | 2 cut(s) | 154, 246 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 113 |
| BstSCI | CCNGG | 1 cut(s) | 70 |
| BstV1I | GCAGC | 2 cut(s) | 19, 223 |
| BstV2I | GAAGAC | 1 cut(s) | 61 |
| BstX2I | RGATCY | 1 cut(s) | 154 |
| BstYI | RGATCY | 1 cut(s) | 154 |
| BtsCI | GGATG | 1 cut(s) | 118 |
| CfoI | GCGC | 1 cut(s) | 19 |
| CviAII | CATG | 2 cut(s) | 401, 480 |
| CviJI | RGCY | 7 cut(s) | 86, 107, 233, 286, 368, 405, 464 |
| CviKI_1 | RGCY | 7 cut(s) | 86, 107, 233, 286, 368, 405, 464 |
| DdeI | CTNAG | 3 cut(s) | 234, 287, 312 |
| DpnI | GATC | 2 cut(s) | 156, 248 |
| DpnII | GATC | 2 cut(s) | 154, 246 |
| DraI | TTTAAA | 1 cut(s) | 205 |
| EcoRI | GAATTC | 1 cut(s) | 421 |
| FaeI | CATG | 2 cut(s) | 404, 483 |
| FatI | CATG | 2 cut(s) | 400, 479 |
| FauI | CCCGC | 1 cut(s) | 353 |
| FauNDI | CATATG | 1 cut(s) | 140 |
| Fnu4HI | GCNGC | 3 cut(s) | 33, 84, 212 |
| FokI | GGATG | 1 cut(s) | 125 |
| Fsp4HI | GCNGC | 3 cut(s) | 33, 84, 212 |
| GlaI | GCGC | 1 cut(s) | 18 |
| GluI | GCNGC | 3 cut(s) | 33, 84, 212 |
| HapII | CCGG | 1 cut(s) | 72 |
| HhaI | GCGC | 1 cut(s) | 19 |
| Hin1II | CATG | 2 cut(s) | 404, 483 |
| Hin6I | GCGC | 1 cut(s) | 17 |
| HinP1I | GCGC | 1 cut(s) | 17 |
| HpaII | CCGG | 1 cut(s) | 72 |
| Hpy166II | GTNNAC | 1 cut(s) | 187 |
| Hpy188I | TCNGA | 1 cut(s) | 112 |
| Hpy188III | TCNNGA | 1 cut(s) | 418 |
| Hpy8I | GTNNAC | 1 cut(s) | 187 |
| HpyAV | CCTTC | 4 cut(s) | 40, 44, 445, 454 |
| HpyCH4V | TGCA | 2 cut(s) | 116, 304 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 113 |
| HpyF3I | CTNAG | 3 cut(s) | 234, 287, 312 |
| Hsp92II | CATG | 2 cut(s) | 404, 483 |
| HspAI | GCGC | 1 cut(s) | 17 |
| Kzo9I | GATC | 2 cut(s) | 154, 246 |
| LpnPI | CCDG | 2 cut(s) | 85, 403 |
| Lsp1109I | GCAGC | 2 cut(s) | 19, 223 |
| LweI | GCATC | 1 cut(s) | 103 |
| MalI | GATC | 2 cut(s) | 156, 248 |
| MboI | GATC | 2 cut(s) | 154, 246 |
| MboII | GAAGA | 2 cut(s) | 66, 466 |
| MflI | RGATCY | 1 cut(s) | 154 |
| MluCI | AATT | 2 cut(s) | 118, 421 |
| MmeI | TCCRAC | 1 cut(s) | 297 |
| MnlI | CCTC | 8 cut(s) | 217, 244, 265, 268, 282, 313, 316, 358 |
| MseI | TTAA | 3 cut(s) | 180, 204, 450 |
| MslI | CAYNNNNRTG | 1 cut(s) | 143 |
| MspI | CCGG | 1 cut(s) | 72 |
| MspR9I | CCNGG | 1 cut(s) | 72 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 113 |
| NciI | CCSGG | 1 cut(s) | 72 |
| NdeI | CATATG | 1 cut(s) | 140 |
| NdeII | GATC | 2 cut(s) | 154, 246 |
| NlaIII | CATG | 2 cut(s) | 404, 483 |
| NlaIV | GGNNCC | 1 cut(s) | 156 |
| PkrI | GCNGC | 3 cut(s) | 34, 85, 213 |
| PspN4I | GGNNCC | 1 cut(s) | 156 |
| PsuI | RGATCY | 1 cut(s) | 154 |
| RseI | CAYNNNNRTG | 1 cut(s) | 143 |
| SaqAI | TTAA | 3 cut(s) | 180, 204, 450 |
| SatI | GCNGC | 3 cut(s) | 33, 84, 212 |
| Sau3AI | GATC | 2 cut(s) | 154, 246 |
| ScrFI | CCNGG | 1 cut(s) | 72 |
| SetI | ASST | 2 cut(s) | 228, 327 |
| SfaNI | GCATC | 1 cut(s) | 103 |
| SgeI | CNNG | 8 cut(s) | 83, 84, 99, 262, 373, 413, 430, 456 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 143 |
| Sse9I | AATT | 2 cut(s) | 118, 421 |
| SsiI | CCGC | 5 cut(s) | 83, 101, 152, 329, 360 |
| StyD4I | CCNGG | 1 cut(s) | 70 |
| TaqI | TCGA | 1 cut(s) | 249 |
| TasI | AATT | 2 cut(s) | 118, 421 |
| TauI | GCSGC | 1 cut(s) | 86 |
| Tru1I | TTAA | 3 cut(s) | 180, 204, 450 |
| Tru9I | TTAA | 3 cut(s) | 180, 204, 450 |
| TseI | GCWGC | 2 cut(s) | 32, 211 |
| TspDTI | ATGAA | 4 cut(s) | 17, 398, 427, 473 |
| TspGWI | ACGGA | 1 cut(s) | 147 |
| XapI | RAATTY | 2 cut(s) | 118, 421 |