Rorug05G0550400

3-oxo-Delta(4,5)-steroid 5-beta-reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000626
Physical Location & Seq
Forward (+)
73855568 .. 73861650
6083 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0550400.1

Sequence Viewer

Length: 1665 bp
ATGATGGCGGAGGCTGGGGTAGCCGGATTGGGGGACATACAGGGCAGCGTATTGGAGGCTAAAGTGACCATGTTGCCCATTGAGGAAACGAAGGAGGAAATGGGCCGGGCCGAGATGTTGAGGAAAGGCGAGACGGTGGCGGTGGCTGACAATGTTAATATGGTCTGTTTGGATGATGGCGATGAGTCTTCGATGGTGAGATTAGGCATAGGGAAGAGCGAGAGTACTAATTCCAGAGATCAAAGCTTTGTTACACGTTCAAAACCTAGAATTATGTATTGTAGTTTTTTTGTGGTCGCCGGAGTTCGTCTGAGGTCGGCCGGAGTTCGCCGGAGCCGGAACTCCCATAGTTTAGCTCGCCTCTCTTCAAATACGTTGTCGTTTTGCTGGACCAGCCTCCCATACCAGAGCTCCATCGCCGGAACTCCTAAGCTCGCCTCTTCATCTCCACATACGTCGTCGTTTTGCTGGACCAGCCTCCCATACGGCCATACCAGAGCTCTATCGCAGTTTTTTTGTTGGGAATTGTTGTCAATGTTGCACTGCGTTGATCTCCCTGAAGATGTTCTAGTGAAGATTCTGTGTCGGTTGCCGGTCAAATATTTGATCCGCTTCACCTGTGTGTCGAAACGGTGGCGTTCTGTTATCACTTCTGACCCTCAGTTTGCCAAATCCCACCTCCAGCTAGCATTACAGCAGGGAACCCTCCGTCATAGAGTCCTCGCCTCTGCCTATTATTTTGACTTTAATTTAGTAAGACTCTACGATGATGTACCCACTCGATTTCAATCATTTGAAGATAATTCTTCAGTCAGAACTCTAACCTACCCAACAGAGGAGAATAATGATCCACAAATAGTTGTTTCCTGCAATGGTTTGGTTCTTCTAGCTGAATCCACTACCAGTCAGTTTAATAACTTGTATATCTGGAACCCATCAACCAGATTTTCCCGCAAAATTCCTCATCTGCCAGAAGGGGACCTATTCTATATAAGTCTTGGTCATGTTTCAGCCACAAATGACTACAAAGTTGTCCTAATACCGCATTCTGGTAGTTCTGGTCATGTTTACTCACTCAGAGAAAAGATTTGGAAAGTTGTTGAAGTTCCTCCTTCACACAGCTGGAGTAGCCCTGTGGGGACTTATTCAAATGGAGCAATTCATTGGGTCAACTACTGCACGCCAGAGAATTCTGAGAGAGTTATCTATGCTTTTGATTTGGCGAAGGAGGAGTTCGGAAAAGTAGCATTGCCTGATTCATTCCAAAATGGATATGACATGAATGGGAGAACGATAAGAACTCAGGTTCATTTAGGAGGATGCCTTTGTGTATCTTCTGATGCCGATCATGGGCTTATTGAATTCTGGGTCATGACAGAGTATGGTGTGCCTGAATCTTGGGTTAAACTCTTTCAGTTTGAGGTAAGGGATTTACGAAAAGTCTCTGCACACTCATACTATGGTTGTAGTCCTATTTTTGTGACAGAAGGCGGCACAGTTTTGATGAAATTTTCTGACAAGATGGAGTTTTTTAAGATCGAACGCTCCAAGGAAGGGAAGCTGGTCTGCAGCCGTCGATATAGGCTTGAGGAGGAGGTGCATGGGTGCAAGTATATATCTCATGCGACTGTATATGATGAAACTCTAGTTTCGATACCCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

554

Amino Acids

62.51

Weight (kDa)

6.79

Isoelectric Point (pI)

45.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 184 - 220 8e-12 F-box domain
F-box-like PF12937 184 - 219 1.1e-07 F-box-like
FBA_3 PF08268 266 - 502 6.2e-18 F-box associated beta propeller domain
FBA_1 PF07734 279 - 552 3.4e-21 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000626)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24220 AT4G24220
fragaria_vesca FvH4_2g04580 FvH4_2g04580 FvH4_2g04580 FvH4_4g06071 FvH4_5g25130
malus_domestica MD05G1094500.v1.1 MD05G1108300.v1.1 MD08G1088400.v1.1 MD15G1073200.v1.1 MD15G1073300.v1.1 MD15G1073400.v1.1 MD15G1073500.v1.1 MD15G1073600.v1.1
prunus_persica Prupe.1G426100_v2.0.a1 Prupe.1G426100_v2.0.a1 Prupe.1G426200_v2.0.a1 Prupe.1G426300_v2.0.a1 Prupe.1G426400_v2.0.a1 Prupe.1G426400_v2.0.a1 Prupe.8G139400_v2.0.a1 Prupe.8G139400_v2.0.a1
pyrus_communis pycom05g09350 pycom08g07190 pycom13g22780 pycom15g06840 pycom15g06850 pycom15g06870
rosa_chinensis RchiOBHm_Chr6g0254161 RchiOBHm_Chr6g0254181 RchiOBHm_Chr6g0254191 RchiOBHm_Chr6g0309171 RchiOBHm_Chr6g0309181 RchiOBHm_Chr6g0309191 RchiOBHm_Chr6g0309201
rosa_laevigata RLG00000010582 RLG00000010583 RLG00000014937 RLG00000014938
rosa_multiflora Rmu_co8060540.1_g000001 Rmu_co8332663.1_g000001 Rmu_sc0000010.1_g000017 Rmu_sc0003711.1_g000008 Rmu_sc0016971.1_g000003 Rmu_sc0020102.1_g000006 Rmu_sc0020102.1_g000008
rosa_roxburghii Rroxscaffold_7G00159570 Rroxscaffold_7G00159580 Rroxscaffold_7G00159590 Rroxscaffold_7G00211100
rosa_rugosa Rorug05G0550400 Rorug06G0370100 Rorug06G0370300
rosa_samantha Rh6AG066900 Rh6AG483200 Rh6AG483300 Rh6AG483400 Rh6BG059800 Rh6BG060000 Rh6BG492200 Rh6BG492300 Rh6BG492500 Rh6CG059700 Rh6CG059800 Rh6CG496900 Rh6CG497000 Rh6CG497200 Rh6CG497400 Rh6DG056700 Rh6DG056800 Rh6DG483800 Rh6DG484000
rosa_wichuraiana Rw6G005860 Rw6G005870 Rw6G042070 Rw6G042080 Rw6G042090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 8, 140, 610, 952, 1043, 1491
AclWI GGATC 2 cut(s) 601, 842
AcoI YGGCCR 2 cut(s) 318, 487
AcsI RAATTY 4 cut(s) 957, 1189, 1361, 1508
AcuI CTGAAG 2 cut(s) 579, 792
AfaI GTAC 2 cut(s) 226, 774
AfiI CCNNNNNNNGG 5 cut(s) 30, 835, 1049, 1350, 1554
AflIII ACRYGT 1 cut(s) 254
AgsI TTSAA 7 cut(s) 261, 369, 788, 797, 1103, 1149, 1361
AleI CACNNNNGTG 1 cut(s) 620
AluBI AGCT 9 cut(s) 246, 356, 411, 433, 500, 685, 890, 1122, 1561
AluI AGCT 9 cut(s) 246, 356, 411, 433, 500, 685, 890, 1122, 1561
Alw21I GWGCWC 2 cut(s) 413, 502
Alw26I GTCTC 2 cut(s) 125, 1447
AlwI GGATC 2 cut(s) 601, 842
AoxI GGCC 4 cut(s) 103, 108, 318, 487
ApeKI GCWGC 2 cut(s) 45, 1569
ApoI RAATTY 4 cut(s) 957, 1189, 1361, 1508
ArsI GACNNNNNNTTYG 4 cut(s) 362, 394, 647, 679
Asp700I GAANNNNTTC 1 cut(s) 564
AspS9I GGNCC 5 cut(s) 103, 108, 390, 471, 979
AsuC2I CCSGG 1 cut(s) 107
AsuHPI GGTGA 2 cut(s) 208, 607
AsuNHI GCTAGC 1 cut(s) 685
AvaII GGWCC 3 cut(s) 390, 471, 979
BanII GRGCYC 2 cut(s) 413, 502
BbsI GAAGAC 1 cut(s) 180
Bbv12I GWGCWC 2 cut(s) 413, 502
BbvI GCAGC 2 cut(s) 57, 1581
BccI CCATC 5 cut(s) 170, 187, 422, 943, 1516
BceAI ACGGC 2 cut(s) 502, 1557
BcnI CCSGG 1 cut(s) 107
BcoDI GTCTC 2 cut(s) 125, 1447
BfaI CTAG 5 cut(s) 267, 569, 686, 887, 1646
BfmI CTRYAG 1 cut(s) 1567
BisI GCNGC 3 cut(s) 46, 1492, 1570
BlsI GCNGC 3 cut(s) 47, 1493, 1571
BmcAI AGTACT 1 cut(s) 226
Bme1390I CCNGG 1 cut(s) 107
Bme18I GGWCC 3 cut(s) 390, 471, 979
BmgT120I GGNCC 5 cut(s) 103, 108, 390, 471, 979
BmiI GGNNCC 4 cut(s) 335, 703, 932, 980
BmrFI CCNGG 1 cut(s) 107
BmsI GCATC 2 cut(s) 1310, 1330
BmtI GCTAGC 1 cut(s) 689
BpiI GAAGAC 1 cut(s) 180
BpmI CTGGAG 2 cut(s) 665, 1144
Bpu10I CCTNAGC 1 cut(s) 429
BpuEI CTTGAG 1 cut(s) 1607
BpuMI CCSGG 1 cut(s) 107
BsaBI GATNNNNATC 2 cut(s) 787, 1344
BsaJI CCNNGG 1 cut(s) 1548
BsaXI ACNNNNNCTCC 2 cut(s) 395, 425
Bsc4I CCNNNNNNNGG 5 cut(s) 30, 835, 1049, 1350, 1554
Bse118I RCCGGY 1 cut(s) 592
Bse1I ACTGG 1 cut(s) 903
Bse3DI GCAATG 2 cut(s) 877, 1247
Bse8I GATNNNNATC 2 cut(s) 787, 1344
BseDI CCNNGG 1 cut(s) 1548
BseGI GGATG 2 cut(s) 178, 1325
BseJI GATNNNNATC 2 cut(s) 787, 1344
BseLI CCNNNNNNNGG 5 cut(s) 30, 835, 1049, 1350, 1554
BseMI GCAATG 2 cut(s) 877, 1247
BseMII CTCAG 5 cut(s) 302, 674, 1090, 1185, 1316
BseNI ACTGG 1 cut(s) 903
BseRI GAGGAG 4 cut(s) 851, 1244, 1604, 1607
BseX3I CGGCCG 1 cut(s) 318
BseXI GCAGC 2 cut(s) 57, 1581
BseYI CCCAGC 1 cut(s) 14
BsgI GTGCAG 2 cut(s) 1162, 1431
Bsh1285I CGRYCG 1 cut(s) 321
BshFI GGCC 4 cut(s) 105, 110, 320, 489
BsiEI CGRYCG 1 cut(s) 321
BsiHKAI GWGCWC 2 cut(s) 413, 502
BsiSI CCGG 8 cut(s) 24, 106, 300, 321, 331, 337, 420, 593
BslFI GGGAC 3 cut(s) 47, 992, 1153
BslI CCNNNNNNNGG 5 cut(s) 30, 835, 1049, 1350, 1554
BsmAI GTCTC 2 cut(s) 125, 1447
BsmBI CGTCTC 1 cut(s) 125
BsmFI GGGAC 3 cut(s) 47, 992, 1153
BsmI GAATGC 1 cut(s) 1045
BsnI GGCC 4 cut(s) 105, 110, 320, 489
Bsp1286I GDGCHC 2 cut(s) 413, 502
Bsp143I GATC 6 cut(s) 238, 550, 606, 847, 1345, 1536
BspACI CCGC 6 cut(s) 8, 140, 610, 952, 1043, 1491
BspANI GGCC 4 cut(s) 105, 110, 320, 489
BspCNI CTCAG 5 cut(s) 303, 673, 1089, 1186, 1315
BspHI TCATGA 1 cut(s) 1371
BspLI GGNNCC 4 cut(s) 335, 703, 932, 980
BspMAI CTGCAG 1 cut(s) 1571
BspOI GCTAGC 1 cut(s) 689
BspPI GGATC 2 cut(s) 601, 842
BspQI GCTCTTC 1 cut(s) 209
BsrDI GCAATG 2 cut(s) 877, 1247
BsrFI RCCGGY 1 cut(s) 592
BsrI ACTGG 1 cut(s) 903
BssAI RCCGGY 1 cut(s) 592
BssECI CCNNGG 1 cut(s) 1548
BssMI GATC 6 cut(s) 238, 550, 606, 847, 1345, 1536
BssT1I CCWWGG 1 cut(s) 1548
Bst4CI ACNGT 4 cut(s) 136, 633, 1498, 1630
Bst6I CTCTTC 3 cut(s) 209, 370, 445
BstC8I GCNNGC 4 cut(s) 358, 435, 687, 1181
BstDEI CTNAG 6 cut(s) 311, 429, 660, 1076, 1194, 1302
BstF5I GGATG 2 cut(s) 178, 1325
BstKTI GATC 6 cut(s) 241, 553, 609, 850, 1348, 1539
BstMAI GTCTC 2 cut(s) 125, 1447
BstMBI GATC 6 cut(s) 238, 550, 606, 847, 1345, 1536
BstMCI CGRYCG 1 cut(s) 321
BstMWI GCNNNNNNNGC 4 cut(s) 20, 393, 474, 1128
BstSCI CCNGG 1 cut(s) 105
BstSFI CTRYAG 1 cut(s) 1567
BstV1I GCAGC 2 cut(s) 57, 1581
BstV2I GAAGAC 1 cut(s) 180
BstZI CGGCCG 1 cut(s) 318
BsuRI GGCC 4 cut(s) 105, 110, 320, 489
BtgZI GCGATG 2 cut(s) 195, 400
BtsCI GGATG 2 cut(s) 178, 1325
BtsI GCAGTG 1 cut(s) 541
BtsIMutI CAGTG 1 cut(s) 541
Cac8I GCNNGC 4 cut(s) 358, 435, 687, 1181
CciI TCATGA 1 cut(s) 1371
Cfr10I RCCGGY 1 cut(s) 592
Cfr13I GGNCC 5 cut(s) 103, 108, 390, 471, 979
Csp6I GTAC 2 cut(s) 225, 773
CviAII CATG 8 cut(s) 70, 1004, 1064, 1279, 1349, 1372, 1601, 1622
CviQI GTAC 2 cut(s) 225, 773
DdeI CTNAG 6 cut(s) 311, 429, 660, 1076, 1194, 1302
DpnI GATC 6 cut(s) 240, 552, 608, 849, 1347, 1538
DpnII GATC 6 cut(s) 238, 550, 606, 847, 1345, 1536
EaeI YGGCCR 2 cut(s) 318, 487
EagI CGGCCG 1 cut(s) 318
Eam1104I CTCTTC 3 cut(s) 209, 370, 445
EarI CTCTTC 3 cut(s) 209, 370, 445
EciI GGCGGA 1 cut(s) 23
Ecl136II GAGCTC 2 cut(s) 411, 500
EclXI CGGCCG 1 cut(s) 318
Eco130I CCWWGG 1 cut(s) 1548
Eco24I GRGCYC 2 cut(s) 413, 502
Eco47I GGWCC 3 cut(s) 390, 471, 979
Eco52I CGGCCG 1 cut(s) 318
Eco53kI GAGCTC 2 cut(s) 411, 500
Eco57I CTGAAG 2 cut(s) 579, 792
EcoICRI GAGCTC 2 cut(s) 411, 500
EcoO109I RGGNCCY 1 cut(s) 979
EcoRI GAATTC 2 cut(s) 1189, 1361
EcoT14I CCWWGG 1 cut(s) 1548
EcoT38I GRGCYC 2 cut(s) 413, 502
ErhI CCWWGG 1 cut(s) 1548
Esp3I CGTCTC 1 cut(s) 125
FaeI CATG 8 cut(s) 73, 1007, 1067, 1282, 1352, 1375, 1604, 1625
FaqI GGGAC 3 cut(s) 47, 992, 1153
FatI CATG 8 cut(s) 69, 1003, 1063, 1278, 1348, 1371, 1600, 1621
FauI CCCGC 1 cut(s) 959
Fnu4HI GCNGC 3 cut(s) 46, 1492, 1570
FokI GGATG 2 cut(s) 185, 1332
FriOI GRGCYC 2 cut(s) 413, 502
Fsp4HI GCNGC 3 cut(s) 46, 1492, 1570
FspBI CTAG 5 cut(s) 267, 569, 686, 887, 1646
GluI GCNGC 3 cut(s) 46, 1492, 1570
GsaI CCCAGC 1 cut(s) 18
GsuI CTGGAG 2 cut(s) 665, 1144
HaeIII GGCC 4 cut(s) 105, 110, 320, 489
HapII CCGG 8 cut(s) 24, 106, 300, 321, 331, 337, 420, 593
Hin1II CATG 8 cut(s) 73, 1007, 1067, 1282, 1352, 1375, 1604, 1625
HincII GTYRAC 1 cut(s) 1171
HindII GTYRAC 1 cut(s) 1171
HindIII AAGCTT 1 cut(s) 244
HinfI GANTC 7 cut(s) 185, 577, 717, 759, 893, 1256, 1394
HpaII CCGG 8 cut(s) 24, 106, 300, 321, 331, 337, 420, 593
HphI GGTGA 2 cut(s) 208, 607
Hpy166II GTNNAC 2 cut(s) 1069, 1171
Hpy188I TCNGA 8 cut(s) 312, 655, 815, 1079, 1195, 1238, 1339, 1516
Hpy188III TCNNGA 3 cut(s) 234, 928, 1372
Hpy8I GTNNAC 2 cut(s) 1069, 1171
Hpy99I CGWCG 3 cut(s) 460, 463, 1578
HpyAV CCTTC 6 cut(s) 85, 968, 1122, 1219, 1481, 1547
HpyCH4III ACNGT 4 cut(s) 136, 633, 1498, 1630
HpyCH4IV ACGT 3 cut(s) 256, 374, 455
HpyCH4V TGCA 7 cut(s) 541, 870, 1179, 1448, 1569, 1600, 1608
HpyF10VI GCNNNNNNNGC 4 cut(s) 20, 393, 474, 1128
HpyF3I CTNAG 6 cut(s) 311, 429, 660, 1076, 1194, 1302
HpySE526I ACGT 3 cut(s) 256, 374, 455
Hsp92II CATG 8 cut(s) 73, 1007, 1067, 1282, 1352, 1375, 1604, 1625
Kzo9I GATC 6 cut(s) 238, 550, 606, 847, 1345, 1536
LguI GCTCTTC 1 cut(s) 209
LmnI GCTCC 4 cut(s) 333, 416, 1154, 1550
Lsp1109I GCAGC 2 cut(s) 57, 1581
LweI GCATC 2 cut(s) 1310, 1330
MaeI CTAG 5 cut(s) 267, 569, 686, 887, 1646
MaeII ACGT 3 cut(s) 256, 374, 455
MaeIII GTNAC 3 cut(s) 64, 250, 1480
MalI GATC 6 cut(s) 240, 552, 608, 849, 1347, 1538
MboI GATC 6 cut(s) 238, 550, 606, 847, 1345, 1536
MhlI GDGCHC 2 cut(s) 413, 502
MlyI GAGTC 3 cut(s) 194, 726, 753
MroXI GAANNNNTTC 1 cut(s) 564
MseI TTAA 5 cut(s) 156, 747, 912, 1404, 1533
MslI CAYNNNNRTG 1 cut(s) 620
MspA1I CMGCKG 1 cut(s) 1122
MspI CCGG 8 cut(s) 24, 106, 300, 321, 331, 337, 420, 593
MspR9I CCNGG 1 cut(s) 107
Mva1269I GAATGC 1 cut(s) 1045
MwoI GCNNNNNNNGC 4 cut(s) 20, 393, 474, 1128
NciI CCSGG 1 cut(s) 107
NdeII GATC 6 cut(s) 238, 550, 606, 847, 1345, 1536
NheI GCTAGC 1 cut(s) 685
NlaIII CATG 8 cut(s) 73, 1007, 1067, 1282, 1352, 1375, 1604, 1625
NlaIV GGNNCC 4 cut(s) 335, 703, 932, 980
NmeAIII GCCGAG 1 cut(s) 136
NmuCI GTSAC 2 cut(s) 64, 1480
OliI CACNNNNGTG 1 cut(s) 620
PagI TCATGA 1 cut(s) 1371
PciSI GCTCTTC 1 cut(s) 209
PctI GAATGC 1 cut(s) 1045
PdmI GAANNNNTTC 1 cut(s) 564
PfeI GAWTC 4 cut(s) 577, 893, 1256, 1394
PkrI GCNGC 3 cut(s) 47, 1493, 1571
PleI GAGTC 3 cut(s) 193, 725, 753
PpsI GAGTC 3 cut(s) 193, 725, 753
PpuMI RGGWCCY 1 cut(s) 979
Psp124BI GAGCTC 2 cut(s) 413, 502
Psp5II RGGWCCY 1 cut(s) 979
PspFI CCCAGC 1 cut(s) 14
PspN4I GGNNCC 4 cut(s) 335, 703, 932, 980
PspPI GGNCC 5 cut(s) 103, 108, 390, 471, 979
PspPPI RGGWCCY 1 cut(s) 979
PstI CTGCAG 1 cut(s) 1571
PvuII CAGCTG 1 cut(s) 1122
RsaI GTAC 2 cut(s) 226, 774
RsaNI GTAC 2 cut(s) 225, 773
RseI CAYNNNNRTG 1 cut(s) 620
SacI GAGCTC 2 cut(s) 413, 502
SapI GCTCTTC 1 cut(s) 209
SaqAI TTAA 5 cut(s) 156, 747, 912, 1404, 1533
SatI GCNGC 3 cut(s) 46, 1492, 1570
Sau3AI GATC 6 cut(s) 238, 550, 606, 847, 1345, 1536
Sau96I GGNCC 5 cut(s) 103, 108, 390, 471, 979
ScaI AGTACT 1 cut(s) 226
SchI GAGTC 3 cut(s) 194, 726, 753
ScrFI CCNGG 1 cut(s) 107
SduI GDGCHC 2 cut(s) 413, 502
SfaNI GCATC 2 cut(s) 1310, 1330
SfcI CTRYAG 1 cut(s) 1567
SinI GGWCC 3 cut(s) 390, 471, 979
SmiMI CAYNNNNRTG 1 cut(s) 620
SmlI CTYRAG 1 cut(s) 1586
SmoI CTYRAG 1 cut(s) 1586
SsiI CCGC 6 cut(s) 8, 140, 610, 952, 1043, 1491
SspI AATATT 1 cut(s) 602
SspMI CTAG 5 cut(s) 267, 569, 686, 887, 1646
SstI GAGCTC 2 cut(s) 413, 502
StyD4I CCNGG 1 cut(s) 105
StyI CCWWGG 1 cut(s) 1548
TaaI ACNGT 4 cut(s) 136, 633, 1498, 1630
TaiI ACGT 3 cut(s) 259, 377, 458
TaqI TCGA 6 cut(s) 191, 626, 781, 1539, 1576, 1652
TatI WGTACW 1 cut(s) 224
TauI GCSGC 1 cut(s) 1494
TfiI GAWTC 4 cut(s) 577, 893, 1256, 1394
Tru1I TTAA 5 cut(s) 156, 747, 912, 1404, 1533
Tru9I TTAA 5 cut(s) 156, 747, 912, 1404, 1533
TscAI CASTG 1 cut(s) 548
TseFI GTSAC 2 cut(s) 64, 1480
TseI GCWGC 2 cut(s) 45, 1569
Tsp45I GTSAC 2 cut(s) 64, 1480
TspDTI ATGAA 7 cut(s) 432, 1151, 1248, 1295, 1298, 1520, 1653
TspGWI ACGGA 1 cut(s) 698
TspRI CASTG 1 cut(s) 548
VpaK11BI GGWCC 3 cut(s) 390, 471, 979
XapI RAATTY 4 cut(s) 957, 1189, 1361, 1508
XmnI GAANNNNTTC 1 cut(s) 564
XspI CTAG 5 cut(s) 267, 569, 686, 887, 1646
ZrmI AGTACT 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.