RchiOBHm_Chr6g0254161

3-oxo-Delta(4,5)-steroid 5-beta-reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000626
Physical Location & Seq
Reverse (-)
9203207 .. 9204097
891 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22796

Sequence Viewer

Length: 744 bp
ATGCAGACGGGTCGGAAGCACTACTTAGGTTCGTTTGAGATGTTGGGGAATGTCCAACCCCATGAACCTCCATGTCACGAAGACCTGCCGAGGCTTAATGCCCCCAATTTTTACTATGTGATGGAGGATATTCTGTTTGAGGAGGTGAAGAAGAAGGAGGGGCTTACATGGTCTGTGCATAGGCCGGGACTCATATTCGGCTTCTCACCTTATAGTTTGATGAATGCGATTGGGACTTTGTGTGTTTATGCTGCGATTTGTAAGCATGAAGGGCAGCCGCTCAGGTTTCCGGGGAGTAAAGGAACTTGGGAAGGATTCTGGGACGCGTCTGATGCGGATATGGTTGCTGAACATCAGATATGGGCAGCTGTGGAGCCTTATGCCAAGAATGAAGCTTTTAATTGCATCAATGGGGATGTGTTCAAGTGGAAGCATTTGTGGGGGATTCTGGCTGAGCAGTTCGAGCTAGTGCCTGCTGGAGTTCACGAGGAGTTGAGTTTTGAGGAGATGATGAAAGATAAAGGGCCAGTGTGGGATGAAATTGTGAGAGAGCATGGTTTGGTGCCTACCAAATTGGAAGAGGTAGGGAACTGGTGGTTTCTGGACACTATGTTTCGGTGGATTGATTCAACTGCGGACAGTATGAACAAGAGCAAAGAGCATGGTTTTGTTGGCTTTAGGAACCCGAAAACTTCCTTTGTTTCTTGGATTGAAAAGATGAAGTCTTTTAGAATTGTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

247

Amino Acids

28.55

Weight (kDa)

5.48

Isoelectric Point (pI)

34.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRISE PF22917 1 - 246 1.8e-16 PRISE-like Rossmann-fold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000626)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24220 AT4G24220
fragaria_vesca FvH4_2g04580 FvH4_2g04580 FvH4_2g04580 FvH4_4g06071 FvH4_5g25130
malus_domestica MD05G1094500.v1.1 MD05G1108300.v1.1 MD08G1088400.v1.1 MD15G1073200.v1.1 MD15G1073300.v1.1 MD15G1073400.v1.1 MD15G1073500.v1.1 MD15G1073600.v1.1
prunus_persica Prupe.1G426100_v2.0.a1 Prupe.1G426100_v2.0.a1 Prupe.1G426200_v2.0.a1 Prupe.1G426300_v2.0.a1 Prupe.1G426400_v2.0.a1 Prupe.1G426400_v2.0.a1 Prupe.8G139400_v2.0.a1 Prupe.8G139400_v2.0.a1
pyrus_communis pycom05g09350 pycom08g07190 pycom13g22780 pycom15g06840 pycom15g06850 pycom15g06870
rosa_chinensis RchiOBHm_Chr6g0254161 RchiOBHm_Chr6g0254181 RchiOBHm_Chr6g0254191 RchiOBHm_Chr6g0309171 RchiOBHm_Chr6g0309181 RchiOBHm_Chr6g0309191 RchiOBHm_Chr6g0309201
rosa_laevigata RLG00000010582 RLG00000010583 RLG00000014937 RLG00000014938
rosa_multiflora Rmu_co8060540.1_g000001 Rmu_co8332663.1_g000001 Rmu_sc0000010.1_g000017 Rmu_sc0003711.1_g000008 Rmu_sc0016971.1_g000003 Rmu_sc0020102.1_g000006 Rmu_sc0020102.1_g000008
rosa_roxburghii Rroxscaffold_7G00159570 Rroxscaffold_7G00159580 Rroxscaffold_7G00159590 Rroxscaffold_7G00211100
rosa_rugosa Rorug05G0550400 Rorug06G0370100 Rorug06G0370300
rosa_samantha Rh6AG066900 Rh6AG483200 Rh6AG483300 Rh6AG483400 Rh6BG059800 Rh6BG060000 Rh6BG492200 Rh6BG492300 Rh6BG492500 Rh6CG059700 Rh6CG059800 Rh6CG496900 Rh6CG497000 Rh6CG497200 Rh6CG497400 Rh6DG056700 Rh6DG056800 Rh6DG483800 Rh6DG484000
rosa_wichuraiana Rw6G005860 Rw6G005870 Rw6G042070 Rw6G042080 Rw6G042090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 93
AccB1I GGYRCC 1 cut(s) 562
AccBSI CCGCTC 1 cut(s) 280
AccII CGCG 1 cut(s) 326
AciI CCGC 3 cut(s) 278, 335, 635
AflIII ACRYGT 1 cut(s) 324
AgsI TTSAA 3 cut(s) 424, 630, 713
AluBI AGCT 3 cut(s) 368, 395, 466
AluI AGCT 3 cut(s) 368, 395, 466
AoxI GGCC 2 cut(s) 182, 524
ApeKI GCWGC 3 cut(s) 251, 274, 365
AspS9I GGNCC 1 cut(s) 524
AsuC2I CCSGG 2 cut(s) 186, 291
AsuHPI GGTGA 2 cut(s) 157, 198
BanI GGYRCC 1 cut(s) 562
BauI CACGAG 1 cut(s) 485
BbsI GAAGAC 1 cut(s) 87
BbvI GCAGC 3 cut(s) 238, 286, 377
BccI CCATC 1 cut(s) 115
BcgI CGANNNNNNTGC 1 cut(s) 27
BcnI CCSGG 2 cut(s) 186, 291
BfaI CTAG 1 cut(s) 467
BfuAI ACCTGC 1 cut(s) 93
BisI GCNGC 4 cut(s) 252, 275, 278, 366
BlpI GCTNAGC 1 cut(s) 453
BlsI GCNGC 4 cut(s) 253, 276, 279, 367
Bme1390I CCNGG 2 cut(s) 186, 291
BmgT120I GGNCC 1 cut(s) 524
BmiI GGNNCC 3 cut(s) 375, 564, 683
BmrFI CCNGG 2 cut(s) 186, 291
BmsI GCATC 2 cut(s) 322, 414
BpiI GAAGAC 1 cut(s) 87
BpmI CTGGAG 1 cut(s) 498
Bpu10I CCTNAGC 1 cut(s) 281
Bpu1102I GCTNAGC 1 cut(s) 453
BpuMI CCSGG 2 cut(s) 186, 291
BsaJI CCNNGG 2 cut(s) 89, 290
Bse1I ACTGG 2 cut(s) 527, 596
BseDI CCNNGG 2 cut(s) 89, 290
BseGI GGATG 2 cut(s) 421, 541
BseMII CTCAG 2 cut(s) 295, 444
BseNI ACTGG 2 cut(s) 527, 596
BseRI GAGGAG 3 cut(s) 155, 503, 518
BseXI GCAGC 3 cut(s) 238, 286, 377
Bsh1236I CGCG 1 cut(s) 326
BshFI GGCC 2 cut(s) 184, 526
BshNI GGYRCC 1 cut(s) 562
BsiSI CCGG 2 cut(s) 185, 290
BslFI GGGAC 3 cut(s) 201, 247, 335
BsmFI GGGAC 3 cut(s) 201, 247, 335
BsmI GAATGC 1 cut(s) 229
BsnI GGCC 2 cut(s) 184, 526
Bsp1720I GCTNAGC 1 cut(s) 453
BspACI CCGC 3 cut(s) 278, 335, 635
BspANI GGCC 2 cut(s) 184, 526
BspCNI CTCAG 2 cut(s) 294, 445
BspFNI CGCG 1 cut(s) 326
BspLI GGNNCC 3 cut(s) 375, 564, 683
BspMI ACCTGC 1 cut(s) 93
BspT107I GGYRCC 1 cut(s) 562
BsrBI CCGCTC 1 cut(s) 280
BsrI ACTGG 2 cut(s) 527, 596
BssECI CCNNGG 2 cut(s) 89, 290
BssSI CACGAG 1 cut(s) 485
Bst2BI CACGAG 1 cut(s) 485
Bst4CI ACNGT 1 cut(s) 641
Bst6I CTCTTC 1 cut(s) 573
BstC8I GCNNGC 1 cut(s) 474
BstDEI CTNAG 3 cut(s) 25, 281, 453
BstF5I GGATG 2 cut(s) 421, 541
BstFNI CGCG 1 cut(s) 326
BstMWI GCNNNNNNNGC 3 cut(s) 271, 332, 463
BstSCI CCNGG 2 cut(s) 184, 289
BstUI CGCG 1 cut(s) 326
BstV1I GCAGC 3 cut(s) 238, 286, 377
BstV2I GAAGAC 1 cut(s) 87
BsuRI GGCC 2 cut(s) 184, 526
BtsCI GGATG 2 cut(s) 421, 541
BtsIMutI CAGTG 1 cut(s) 534
BveI ACCTGC 1 cut(s) 93
Cac8I GCNNGC 1 cut(s) 474
Cfr13I GGNCC 1 cut(s) 524
CseI GACGC 2 cut(s) 315, 332
CviAII CATG 6 cut(s) 62, 72, 168, 266, 554, 662
DdeI CTNAG 3 cut(s) 25, 281, 453
Eam1104I CTCTTC 1 cut(s) 573
EarI CTCTTC 1 cut(s) 573
FaeI CATG 6 cut(s) 65, 75, 171, 269, 557, 665
FalI AAGNNNNNCTT 2 cut(s) 8, 40
FaqI GGGAC 3 cut(s) 201, 247, 335
FatI CATG 6 cut(s) 61, 71, 167, 265, 553, 661
Fnu4HI GCNGC 4 cut(s) 252, 275, 278, 366
FokI GGATG 2 cut(s) 428, 548
Fsp4HI GCNGC 4 cut(s) 252, 275, 278, 366
FspBI CTAG 1 cut(s) 467
GluI GCNGC 4 cut(s) 252, 275, 278, 366
GsuI CTGGAG 1 cut(s) 498
HaeIII GGCC 2 cut(s) 184, 526
HapII CCGG 2 cut(s) 185, 290
HgaI GACGC 2 cut(s) 315, 332
Hin1II CATG 6 cut(s) 65, 75, 171, 269, 557, 665
HindIII AAGCTT 1 cut(s) 393
HinfI GANTC 4 cut(s) 189, 315, 445, 626
HpaII CCGG 2 cut(s) 185, 290
HphI GGTGA 2 cut(s) 157, 198
Hpy166II GTNNAC 1 cut(s) 484
Hpy188I TCNGA 3 cut(s) 15, 331, 357
Hpy188III TCNNGA 3 cut(s) 77, 485, 602
Hpy8I GTNNAC 1 cut(s) 484
HpyAV CCTTC 3 cut(s) 148, 263, 305
HpyCH4III ACNGT 1 cut(s) 641
HpyCH4V TGCA 3 cut(s) 4, 178, 405
HpyF10VI GCNNNNNNNGC 3 cut(s) 271, 332, 463
HpyF3I CTNAG 3 cut(s) 25, 281, 453
Hsp92II CATG 6 cut(s) 65, 75, 171, 269, 557, 665
LmnI GCTCC 1 cut(s) 373
Lsp1109I GCAGC 3 cut(s) 238, 286, 377
LweI GCATC 2 cut(s) 322, 414
MaeI CTAG 1 cut(s) 467
MaeIII GTNAC 1 cut(s) 74
MbiI CCGCTC 1 cut(s) 280
MboII GAAGA 4 cut(s) 92, 160, 163, 590
MluCI AATT 5 cut(s) 106, 400, 540, 572, 732
MluI ACGCGT 1 cut(s) 324
MlyI GAGTC 1 cut(s) 183
MmeI TCCRAC 1 cut(s) 79
MnlI CCTC 9 cut(s) 78, 84, 118, 133, 136, 151, 481, 496, 574
MseI TTAA 2 cut(s) 96, 399
MspA1I CMGCKG 1 cut(s) 368
MspI CCGG 2 cut(s) 185, 290
MspR9I CCNGG 2 cut(s) 186, 291
Mva1269I GAATGC 1 cut(s) 229
MvnI CGCG 1 cut(s) 326
MwoI GCNNNNNNNGC 3 cut(s) 271, 332, 463
NciI CCSGG 2 cut(s) 186, 291
NlaIII CATG 6 cut(s) 65, 75, 171, 269, 557, 665
NlaIV GGNNCC 3 cut(s) 375, 564, 683
NmeAIII GCCGAG 1 cut(s) 114
NmuCI GTSAC 1 cut(s) 74
PctI GAATGC 1 cut(s) 229
PfeI GAWTC 3 cut(s) 315, 445, 626
PkrI GCNGC 4 cut(s) 253, 276, 279, 367
PleI GAGTC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 183
PspN4I GGNNCC 3 cut(s) 375, 564, 683
PspPI GGNCC 1 cut(s) 524
PvuII CAGCTG 1 cut(s) 368
SaqAI TTAA 2 cut(s) 96, 399
SatI GCNGC 4 cut(s) 252, 275, 278, 366
Sau96I GGNCC 1 cut(s) 524
SchI GAGTC 1 cut(s) 183
ScrFI CCNGG 2 cut(s) 186, 291
SfaNI GCATC 2 cut(s) 322, 414
Sse9I AATT 5 cut(s) 106, 400, 540, 572, 732
SsiI CCGC 3 cut(s) 278, 335, 635
SspMI CTAG 1 cut(s) 467
StyD4I CCNGG 2 cut(s) 184, 289
TaaI ACNGT 1 cut(s) 641
TaqI TCGA 1 cut(s) 462
TasI AATT 5 cut(s) 106, 400, 540, 572, 732
TauI GCSGC 1 cut(s) 280
TfiI GAWTC 3 cut(s) 315, 445, 626
Tru1I TTAA 2 cut(s) 96, 399
Tru9I TTAA 2 cut(s) 96, 399
TscAI CASTG 1 cut(s) 534
TseFI GTSAC 1 cut(s) 74
TseI GCWGC 3 cut(s) 251, 274, 365
Tsp45I GTSAC 1 cut(s) 74
TspDTI ATGAA 8 cut(s) 78, 236, 282, 405, 527, 552, 659, 734
TspRI CASTG 1 cut(s) 534
XspI CTAG 1 cut(s) 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.