MD05G1094500.v1.1

3-oxo-Delta(4,5)-steroid 5-beta-reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000626
Physical Location & Seq
Forward (+)
20039460 .. 20041173
1714 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1094500.v1.1.491

Sequence Viewer

Length: 1176 bp
ATGAGCTGGTGGTGGGCAGGAGCCATTGGAGCTGCAAAGAAAACTTTTGATCCCAATGATGTGCCGCCCAAATACCAGAGCGTGGCTCTGGTTCTCGGCGTCACTGGGATAGTCGGCAATAGCCTTGCCGAGATTCTCCCCCTGGCCGACACACCCGGCGGGCCATGGAAGGTATATGGTGTAGCCCGCCGGCCTCGTCCGGCGTGGAACGCTGATCATCCTGTTGAATACATTCAGTGTGATCTCTCAGACCCTCATCAAACACATTCTCAGCTGTCCCAGCTGACCGATGTCACTCATGTATTTTATGTGACGTGGGCAAGTAAGCCCACGGAAGCTGAGAACTGTGAGGTCAACGGCAACATGTTTCGGAACTTGCTGGATGCTGTGATCTCAAATGCACCAAATCTGCAGCACATATGCTTGCAAACGGGCCGGAAGCATTATATCGGTCCGTTTGAGATATTGGGCAAGGTCGAGCCTCATGAGCCACCGTTTCACGAAGACCTCCCGAGGCTTGACGTGCCCAATTTTTACTACACATTGGAGGACATTTTGTTTGAGGAGGTGAAGAAAAAGGAGGGTTTGACATGGTCAGTGCACAGGCCTAGCGTGATATTCGGGTTTTCGCCCTATAGCTTGATGAACATTGTGGGGAGCCTCTGCATTTATGCCGCAATATGTAAGCACGAGGGGAAGAAACTGAGGTTTACGGGGAGCAGAGGGGCGTGGAACGGGTACTGGGATGTCTCGGACGCCGACTTGATAGCCGAGCACCAGATTTGGGCAGCAGTTGATCCATATGCCAAGAATGAGGCTTTCAATTGCAGCAATGGGGATGTGGTTAAGTGGAAGCATTTGTTCAGGGTTTTGGCAGAGCAGTTCGAGCTTGATATTGAGGACGTTGGCGAGGAGGGCGGATCGGAGGCGAGCTTGGAGGAGATGATGAAAGATAAGGGGCCGGTGTGGGATGAGATTGTGAAAGAGAAGGGTCTGGTTCCTACCAAGCTGGAAGAAGTTGGTAACTGGTGGTTTGTGGATCTTATGTTTGGAGTTGAGACAAGTCTGGACAGCATGAACAAGAGCAAAGAACATGGTTTTGTGGGGTTCAGGAATTCAAAGACATCGTTTTTAACTTGGATTGACAGGATGAAATCTTACAGACTTGTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

392

Amino Acids

44.23

Weight (kDa)

5.35

Isoelectric Point (pI)

33.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 29 - 253 1.9e-08 NAD dependent epimerase/dehydratase family
PRISE PF22917 88 - 391 6.2e-25 PRISE-like Rossmann-fold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000626)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24220 AT4G24220
fragaria_vesca FvH4_2g04580 FvH4_2g04580 FvH4_2g04580 FvH4_4g06071 FvH4_5g25130
malus_domestica MD05G1094500.v1.1 MD05G1108300.v1.1 MD08G1088400.v1.1 MD15G1073200.v1.1 MD15G1073300.v1.1 MD15G1073400.v1.1 MD15G1073500.v1.1 MD15G1073600.v1.1
prunus_persica Prupe.1G426100_v2.0.a1 Prupe.1G426100_v2.0.a1 Prupe.1G426200_v2.0.a1 Prupe.1G426300_v2.0.a1 Prupe.1G426400_v2.0.a1 Prupe.1G426400_v2.0.a1 Prupe.8G139400_v2.0.a1 Prupe.8G139400_v2.0.a1
pyrus_communis pycom05g09350 pycom08g07190 pycom13g22780 pycom15g06840 pycom15g06850 pycom15g06870
rosa_chinensis RchiOBHm_Chr6g0254161 RchiOBHm_Chr6g0254181 RchiOBHm_Chr6g0254191 RchiOBHm_Chr6g0309171 RchiOBHm_Chr6g0309181 RchiOBHm_Chr6g0309191 RchiOBHm_Chr6g0309201
rosa_laevigata RLG00000010582 RLG00000010583 RLG00000014937 RLG00000014938
rosa_multiflora Rmu_co8060540.1_g000001 Rmu_co8332663.1_g000001 Rmu_sc0000010.1_g000017 Rmu_sc0003711.1_g000008 Rmu_sc0016971.1_g000003 Rmu_sc0020102.1_g000006 Rmu_sc0020102.1_g000008
rosa_roxburghii Rroxscaffold_7G00159570 Rroxscaffold_7G00159580 Rroxscaffold_7G00159590 Rroxscaffold_7G00211100
rosa_rugosa Rorug05G0550400 Rorug06G0370100 Rorug06G0370300
rosa_samantha Rh6AG066900 Rh6AG483200 Rh6AG483300 Rh6AG483400 Rh6BG059800 Rh6BG060000 Rh6BG492200 Rh6BG492300 Rh6BG492500 Rh6CG059700 Rh6CG059800 Rh6CG496900 Rh6CG497000 Rh6CG497200 Rh6CG497400 Rh6DG056700 Rh6DG056800 Rh6DG483800 Rh6DG484000
rosa_wichuraiana Rw6G005860 Rw6G005870 Rw6G042070 Rw6G042080 Rw6G042090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 82
AciI CCGC 5 cut(s) 65, 159, 187, 675, 918
AclWI GGATC 4 cut(s) 44, 791, 928, 1047
AcoI YGGCCR 1 cut(s) 144
AcsI RAATTY 1 cut(s) 1114
AcyI GRCGYC 2 cut(s) 99, 756
AfaI GTAC 1 cut(s) 740
AfiI CCNNNNNNNGG 2 cut(s) 82, 784
AflIII ACRYGT 1 cut(s) 363
AgsI TTSAA 3 cut(s) 227, 823, 1119
AjiI CACGTC 2 cut(s) 315, 523
AjnI CCWGG 1 cut(s) 141
AluBI AGCT 9 cut(s) 6, 32, 274, 283, 338, 639, 889, 933, 1009
AluI AGCT 9 cut(s) 6, 32, 274, 283, 338, 639, 889, 933, 1009
Alw21I GWGCWC 2 cut(s) 603, 777
Alw26I GTCTC 2 cut(s) 754, 1052
Alw44I GTGCAC 1 cut(s) 599
AlwI GGATC 4 cut(s) 44, 791, 928, 1047
Ama87I CYCGRG 1 cut(s) 511
AoxI GGCC 6 cut(s) 144, 161, 191, 433, 605, 959
ApaLI GTGCAC 1 cut(s) 599
ApeKI GCWGC 4 cut(s) 32, 412, 788, 828
ApoI RAATTY 1 cut(s) 1114
ArsI GACNNNNNNTTYG 2 cut(s) 542, 574
Asp700I GAANNNNTTC 1 cut(s) 231
AspS9I GGNCC 4 cut(s) 161, 433, 452, 959
AsuC2I CCSGG 1 cut(s) 156
AsuHPI GGTGA 1 cut(s) 580
AvaI CYCGRG 1 cut(s) 511
AvaII GGWCC 1 cut(s) 452
BaeGI GKGCMC 2 cut(s) 528, 603
BauI CACGAG 1 cut(s) 689
BbsI GAAGAC 1 cut(s) 510
Bbv12I GWGCWC 2 cut(s) 603, 777
BbvI GCAGC 4 cut(s) 19, 424, 800, 840
BceAI ACGGC 1 cut(s) 373
BciT130I CCWGG 1 cut(s) 143
BclI TGATCA 1 cut(s) 214
BcnI CCSGG 1 cut(s) 156
BcoDI GTCTC 2 cut(s) 754, 1052
BfaI CTAG 1 cut(s) 609
BfmI CTRYAG 2 cut(s) 410, 634
BisI GCNGC 6 cut(s) 33, 65, 413, 675, 789, 829
BlsI GCNGC 6 cut(s) 34, 66, 414, 676, 790, 830
Bme1390I CCNGG 2 cut(s) 143, 156
Bme18I GGWCC 1 cut(s) 452
BmeT110I CYCGRG 1 cut(s) 511
BmgBI CACGTC 2 cut(s) 315, 523
BmgT120I GGNCC 4 cut(s) 161, 433, 452, 959
BmiI GGNNCC 4 cut(s) 22, 659, 960, 999
BmrFI CCNGG 2 cut(s) 143, 156
BmrI ACTGGG 2 cut(s) 114, 751
BmsI GCATC 1 cut(s) 373
BmuI ACTGGG 2 cut(s) 114, 751
BoxI GACNNNNGTC 1 cut(s) 290
BpiI GAAGAC 1 cut(s) 510
BplI GAGNNNNNCTC 2 cut(s) 70, 102
BpuMI CCSGG 1 cut(s) 156
BsaHI GRCGYC 2 cut(s) 99, 756
BsaJI CCNNGG 4 cut(s) 141, 164, 330, 512
Bsc4I CCNNNNNNNGG 2 cut(s) 82, 784
Bse118I RCCGGY 2 cut(s) 189, 961
Bse1I ACTGG 3 cut(s) 109, 746, 1031
Bse3DI GCAATG 1 cut(s) 838
BseBI CCWGG 1 cut(s) 143
BseDI CCNNGG 4 cut(s) 141, 164, 330, 512
BseGI GGATG 6 cut(s) 217, 388, 751, 844, 976, 1155
BseLI CCNNNNNNNGG 2 cut(s) 82, 784
BseMI GCAATG 1 cut(s) 838
BseMII CTCAG 4 cut(s) 261, 284, 330, 695
BseNI ACTGG 3 cut(s) 109, 746, 1031
BseRI GAGGAG 3 cut(s) 578, 926, 953
BseSI GKGCMC 2 cut(s) 528, 603
BseXI GCAGC 4 cut(s) 19, 424, 800, 840
BseYI CCCAGC 1 cut(s) 279
BshFI GGCC 6 cut(s) 146, 163, 193, 435, 607, 961
BsiHKAI GWGCWC 2 cut(s) 603, 777
BsiHKCI CYCGRG 1 cut(s) 511
BsiSI CCGG 5 cut(s) 156, 190, 200, 436, 962
BslFI GGGAC 1 cut(s) 262
BslI CCNNNNNNNGG 2 cut(s) 82, 784
BsmAI GTCTC 2 cut(s) 754, 1052
BsmFI GGGAC 1 cut(s) 262
BsnI GGCC 6 cut(s) 146, 163, 193, 435, 607, 961
BsoBI CYCGRG 1 cut(s) 511
Bsp1286I GDGCHC 3 cut(s) 528, 603, 777
Bsp143I GATC 7 cut(s) 49, 214, 241, 390, 796, 920, 1039
Bsp19I CCATGG 1 cut(s) 164
BspACI CCGC 5 cut(s) 65, 159, 187, 675, 918
BspANI GGCC 6 cut(s) 146, 163, 193, 435, 607, 961
BspCNI CTCAG 4 cut(s) 260, 283, 331, 696
BspHI TCATGA 1 cut(s) 484
BspLI GGNNCC 4 cut(s) 22, 659, 960, 999
BspMAI CTGCAG 1 cut(s) 414
BspPI GGATC 4 cut(s) 44, 791, 928, 1047
BsrDI GCAATG 1 cut(s) 838
BsrFI RCCGGY 2 cut(s) 189, 961
BsrI ACTGG 3 cut(s) 109, 746, 1031
BssAI RCCGGY 2 cut(s) 189, 961
BssECI CCNNGG 4 cut(s) 141, 164, 330, 512
BssMI GATC 7 cut(s) 49, 214, 241, 390, 796, 920, 1039
BssNI GRCGYC 2 cut(s) 99, 756
BssSI CACGAG 1 cut(s) 689
BssT1I CCWWGG 1 cut(s) 164
Bst2BI CACGAG 1 cut(s) 689
Bst2UI CCWGG 1 cut(s) 143
Bst4CI ACNGT 2 cut(s) 347, 495
BstACI GRCGYC 2 cut(s) 99, 756
BstC8I GCNNGC 5 cut(s) 161, 187, 191, 425, 931
BstDEI CTNAG 4 cut(s) 247, 270, 339, 704
BstDSI CCRYGG 2 cut(s) 164, 330
BstF5I GGATG 6 cut(s) 217, 388, 751, 844, 976, 1155
BstKTI GATC 7 cut(s) 52, 217, 244, 393, 799, 923, 1042
BstMAI GTCTC 2 cut(s) 754, 1052
BstMBI GATC 7 cut(s) 49, 214, 241, 390, 796, 920, 1039
BstMWI GCNNNNNNNGC 7 cut(s) 29, 209, 280, 487, 523, 886, 915
BstNI CCWGG 1 cut(s) 143
BstNSI RCATGY 1 cut(s) 367
BstPAI GACNNNNGTC 1 cut(s) 290
BstSCI CCNGG 2 cut(s) 141, 154
BstSFI CTRYAG 2 cut(s) 410, 634
BstSLI GKGCMC 2 cut(s) 528, 603
BstV1I GCAGC 4 cut(s) 19, 424, 800, 840
BstV2I GAAGAC 1 cut(s) 510
BstX2I RGATCY 1 cut(s) 1039
BstYI RGATCY 1 cut(s) 1039
BsuRI GGCC 6 cut(s) 146, 163, 193, 435, 607, 961
BtgI CCRYGG 2 cut(s) 164, 330
BtrI CACGTC 2 cut(s) 315, 523
BtsCI GGATG 6 cut(s) 217, 388, 751, 844, 976, 1155
BtsIMutI CAGTG 3 cut(s) 102, 242, 603
Cac8I GCNNGC 5 cut(s) 161, 187, 191, 425, 931
CciI TCATGA 1 cut(s) 484
Cfr10I RCCGGY 2 cut(s) 189, 961
Cfr13I GGNCC 4 cut(s) 161, 433, 452, 959
CpoI CGGWCCG 1 cut(s) 452
CseI GACGC 2 cut(s) 88, 764
Csp6I GTAC 1 cut(s) 739
CspI CGGWCCG 1 cut(s) 452
CviAII CATG 7 cut(s) 165, 299, 364, 485, 591, 1075, 1094
CviQI GTAC 1 cut(s) 739
DdeI CTNAG 4 cut(s) 247, 270, 339, 704
DpnI GATC 7 cut(s) 51, 216, 243, 392, 798, 922, 1041
DpnII GATC 7 cut(s) 49, 214, 241, 390, 796, 920, 1039
EaeI YGGCCR 1 cut(s) 144
EciI GGCGGA 1 cut(s) 933
Eco130I CCWWGG 1 cut(s) 164
Eco147I AGGCCT 1 cut(s) 607
Eco47I GGWCC 1 cut(s) 452
Eco88I CYCGRG 1 cut(s) 511
EcoRI GAATTC 1 cut(s) 1114
EcoRII CCWGG 1 cut(s) 141
EcoT14I CCWWGG 1 cut(s) 164
ErhI CCWWGG 1 cut(s) 164
FaeI CATG 7 cut(s) 168, 302, 367, 488, 594, 1078, 1097
FaqI GGGAC 1 cut(s) 262
FatI CATG 7 cut(s) 164, 298, 363, 484, 590, 1074, 1093
FauI CCCGC 2 cut(s) 152, 194
FauNDI CATATG 2 cut(s) 419, 802
FbaI TGATCA 1 cut(s) 214
Fnu4HI GCNGC 6 cut(s) 33, 65, 413, 675, 789, 829
FokI GGATG 6 cut(s) 204, 395, 758, 851, 983, 1162
Fsp4HI GCNGC 6 cut(s) 33, 65, 413, 675, 789, 829
FspBI CTAG 1 cut(s) 609
GluI GCNGC 6 cut(s) 33, 65, 413, 675, 789, 829
GsaI CCCAGC 1 cut(s) 283
HaeIII GGCC 6 cut(s) 146, 163, 193, 435, 607, 961
HapII CCGG 5 cut(s) 156, 190, 200, 436, 962
HgaI GACGC 2 cut(s) 88, 764
Hin1I GRCGYC 2 cut(s) 99, 756
Hin1II CATG 7 cut(s) 168, 302, 367, 488, 594, 1078, 1097
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HinfI GANTC 1 cut(s) 133
HpaII CCGG 5 cut(s) 156, 190, 200, 436, 962
HphI GGTGA 1 cut(s) 580
Hpy166II GTNNAC 3 cut(s) 355, 601, 711
Hpy188I TCNGA 4 cut(s) 250, 372, 754, 925
Hpy188III TCNNGA 5 cut(s) 485, 500, 511, 1067, 1111
Hpy8I GTNNAC 3 cut(s) 355, 601, 711
HpyAV CCTTC 2 cut(s) 163, 982
HpyCH4III ACNGT 2 cut(s) 347, 495
HpyCH4IV ACGT 3 cut(s) 314, 522, 903
HpyCH4V TGCA 7 cut(s) 35, 401, 412, 427, 601, 666, 828
HpyF10VI GCNNNNNNNGC 7 cut(s) 29, 209, 280, 487, 523, 886, 915
HpyF3I CTNAG 4 cut(s) 247, 270, 339, 704
HpySE526I ACGT 3 cut(s) 314, 522, 903
Hsp92I GRCGYC 2 cut(s) 99, 756
Hsp92II CATG 7 cut(s) 168, 302, 367, 488, 594, 1078, 1097
KroI GCCGGC 1 cut(s) 189
KroNI GCCGGC 1 cut(s) 191
Ksp22I TGATCA 1 cut(s) 214
Kzo9I GATC 7 cut(s) 49, 214, 241, 390, 796, 920, 1039
LmnI GCTCC 4 cut(s) 20, 29, 657, 717
Lsp1109I GCAGC 4 cut(s) 19, 424, 800, 840
LweI GCATC 1 cut(s) 373
MaeI CTAG 1 cut(s) 609
MaeII ACGT 3 cut(s) 314, 522, 903
MaeIII GTNAC 4 cut(s) 100, 292, 310, 1022
MalI GATC 7 cut(s) 51, 216, 243, 392, 798, 922, 1041
MboI GATC 7 cut(s) 49, 214, 241, 390, 796, 920, 1039
MboII GAAGA 4 cut(s) 515, 583, 709, 1025
MfeI CAATTG 1 cut(s) 823
MflI RGATCY 1 cut(s) 1039
MhlI GDGCHC 3 cut(s) 528, 603, 777
MluCI AATT 3 cut(s) 529, 823, 1114
MroNI GCCGGC 1 cut(s) 189
MroXI GAANNNNTTC 1 cut(s) 231
MseI TTAA 2 cut(s) 846, 1133
MspA1I CMGCKG 2 cut(s) 274, 283
MspI CCGG 5 cut(s) 156, 190, 200, 436, 962
MspR9I CCNGG 2 cut(s) 143, 156
MunI CAATTG 1 cut(s) 823
MvaI CCWGG 1 cut(s) 143
MwoI GCNNNNNNNGC 7 cut(s) 29, 209, 280, 487, 523, 886, 915
NaeI GCCGGC 1 cut(s) 191
NciI CCSGG 1 cut(s) 156
NcoI CCATGG 1 cut(s) 164
NdeI CATATG 2 cut(s) 419, 802
NdeII GATC 7 cut(s) 49, 214, 241, 390, 796, 920, 1039
NgoMIV GCCGGC 1 cut(s) 189
NlaIII CATG 7 cut(s) 168, 302, 367, 488, 594, 1078, 1097
NlaIV GGNNCC 4 cut(s) 22, 659, 960, 999
NmeAIII GCCGAG 3 cut(s) 75, 154, 796
NmuCI GTSAC 3 cut(s) 100, 292, 310
NspI RCATGY 1 cut(s) 367
PagI TCATGA 1 cut(s) 484
PceI AGGCCT 1 cut(s) 607
PciI ACATGT 1 cut(s) 363
PdiI GCCGGC 1 cut(s) 191
PdmI GAANNNNTTC 1 cut(s) 231
PfeI GAWTC 1 cut(s) 133
PflFI GACNNNGTC 1 cut(s) 592
PflMI CCANNNNNTGG 1 cut(s) 82
PkrI GCNGC 6 cut(s) 34, 66, 414, 676, 790, 830
PscI ACATGT 1 cut(s) 363
PshAI GACNNNNGTC 1 cut(s) 290
Psp6I CCWGG 1 cut(s) 141
PspFI CCCAGC 1 cut(s) 279
PspGI CCWGG 1 cut(s) 141
PspN4I GGNNCC 4 cut(s) 22, 659, 960, 999
PspPI GGNCC 4 cut(s) 161, 433, 452, 959
PstI CTGCAG 1 cut(s) 414
PsuI RGATCY 1 cut(s) 1039
PsyI GACNNNGTC 1 cut(s) 592
PvuII CAGCTG 2 cut(s) 274, 283
RsaI GTAC 1 cut(s) 740
RsaNI GTAC 1 cut(s) 739
Rsr2I CGGWCCG 1 cut(s) 452
RsrII CGGWCCG 1 cut(s) 452
SaqAI TTAA 2 cut(s) 846, 1133
SatI GCNGC 6 cut(s) 33, 65, 413, 675, 789, 829
Sau3AI GATC 7 cut(s) 49, 214, 241, 390, 796, 920, 1039
Sau96I GGNCC 4 cut(s) 161, 433, 452, 959
ScrFI CCNGG 2 cut(s) 143, 156
SduI GDGCHC 3 cut(s) 528, 603, 777
SfaNI GCATC 1 cut(s) 373
SfcI CTRYAG 2 cut(s) 410, 634
SinI GGWCC 1 cut(s) 452
Sse9I AATT 3 cut(s) 529, 823, 1114
SseBI AGGCCT 1 cut(s) 607
SsiI CCGC 5 cut(s) 65, 159, 187, 675, 918
SspMI CTAG 1 cut(s) 609
StuI AGGCCT 1 cut(s) 607
StyD4I CCNGG 2 cut(s) 141, 154
StyI CCWWGG 1 cut(s) 164
TaaI ACNGT 2 cut(s) 347, 495
TaiI ACGT 3 cut(s) 317, 525, 906
TaqI TCGA 2 cut(s) 477, 885
TaqII GACCGA 2 cut(s) 302, 440
TasI AATT 3 cut(s) 529, 823, 1114
TauI GCSGC 2 cut(s) 67, 677
TfiI GAWTC 1 cut(s) 133
Tru1I TTAA 2 cut(s) 846, 1133
Tru9I TTAA 2 cut(s) 846, 1133
TscAI CASTG 3 cut(s) 109, 242, 603
TseFI GTSAC 3 cut(s) 100, 292, 310
TseI GCWGC 4 cut(s) 32, 412, 788, 828
Tsp45I GTSAC 3 cut(s) 100, 292, 310
TspDTI ATGAA 4 cut(s) 659, 962, 1091, 1166
TspGWI ACGGA 2 cut(s) 347, 444
TspRI CASTG 3 cut(s) 109, 242, 603
Tth111I GACNNNGTC 1 cut(s) 592
Van91I CCANNNNNTGG 1 cut(s) 82
VneI GTGCAC 1 cut(s) 599
VpaK11BI GGWCC 1 cut(s) 452
XapI RAATTY 1 cut(s) 1114
XceI RCATGY 1 cut(s) 367
XmnI GAANNNNTTC 1 cut(s) 231
XspI CTAG 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.