Rh6BG059800

3-oxo-Delta(4,5)-steroid 5-beta-reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000626
Physical Location & Seq
Reverse (-)
9474611 .. 9475842
1232 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG059800.1

Sequence Viewer

Length: 1167 bp
ATGAGTTGGTGGTGGGCAAGAGCTGTAGGAGCTGCAAAGAAAAAGTTTGATCCCGCCGATGCACCGCCCAAATACCAGGGCGTGGCCCTGGTACTTGGGGTTACTGGACTCGTCGGAAACAGCCTTGCCGAGATTCTTCCACTGCCCGACACTCCCGGCGGGCCGTGGAAGGTCTACGGAGTGGCCCGGCGGCCCCGGCCGCAGTGGAACGCTGACCACCCGATTCAGTACATCCAGTGTGACCTCTCTGACTCTACACAGACCGAGGCAGAGCTCTCCCAGCTCACTGATGTCACTCACATTTTTTATGTCACGTGGACGAGCAAGCCAACTGAAGCTGAGAACTGTGAGGCTAATGGCAAGATGTTGAGCAATGTGTTGAATGCTGTGATCCCAAACGCGCCGAATTTGCAGCACATATGTATGCAGACGGGTCGGAAGCACTACTTAGGTTCGTTTGAGAAGTTGGGGAATGTCCAACCCCATGAACCTCCATGTCACGAAGACCTGCCGAGGCTTAATGCCCCCAATTTTTACTATGTGATGGAGGATATTCTGTTTGAGGAGGTGAAGAAGAAGGAGGGGCTTACATGGTCTGTGCATAGGCCGGGACTCATATTCGGCTTCTCACCTTATAGTTTGATGAATGCGATTGGGACTTTGTGTGTTTATGCTGCGATTTGTAAGCATGAAGGGCAGCCGCTCAGGTTTCCGGGGAGTAAAGGAACTTGGGAAGGATTCTGGGACGCGTCTGATGCGGATATGGTTGCTGAACATGAGATATGGGCAGCTGTGGAGCCTTATGCCAAGAATGAAGCTTTTAATTGCATCAATGGGGATGTGTTCAAGTGGAAGCATTTGTGGGGGATTCTGGCTGAGCAGTTCGAGCTAGTGCCCGCTGGAGTTCACGAGGAGTTGAGTTTTGAGGAGATGATGAAAGATAAAGGGCCAGTGTGGGATGAAATTGTGAGAGAGCATGGTTTGGTGCCTACCAAATTGGAAGAGGTAGGGAACTGGTGGTTTCTGGACATTATGTTTCGACGGATTGATTCAACTGCGGACAGTATGAACAAGAGCAAAGAGCATGGTTTTGTTGGCTTTAGGAACCCGAAAACTTCCTTTGTTTCTTGGATTGACAAGATGAAGTCTTTTAGAATTGTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

388

Amino Acids

43.96

Weight (kDa)

5.66

Isoelectric Point (pI)

35.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 29 - 208 7.6e-07 NAD dependent epimerase/dehydratase family
PRISE PF22917 86 - 388 3.7e-24 PRISE-like Rossmann-fold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000626)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24220 AT4G24220
fragaria_vesca FvH4_2g04580 FvH4_2g04580 FvH4_2g04580 FvH4_4g06071 FvH4_5g25130
malus_domestica MD05G1094500.v1.1 MD05G1108300.v1.1 MD08G1088400.v1.1 MD15G1073200.v1.1 MD15G1073300.v1.1 MD15G1073400.v1.1 MD15G1073500.v1.1 MD15G1073600.v1.1
prunus_persica Prupe.1G426100_v2.0.a1 Prupe.1G426100_v2.0.a1 Prupe.1G426200_v2.0.a1 Prupe.1G426300_v2.0.a1 Prupe.1G426400_v2.0.a1 Prupe.1G426400_v2.0.a1 Prupe.8G139400_v2.0.a1 Prupe.8G139400_v2.0.a1
pyrus_communis pycom05g09350 pycom08g07190 pycom13g22780 pycom15g06840 pycom15g06850 pycom15g06870
rosa_chinensis RchiOBHm_Chr6g0254161 RchiOBHm_Chr6g0254181 RchiOBHm_Chr6g0254191 RchiOBHm_Chr6g0309171 RchiOBHm_Chr6g0309181 RchiOBHm_Chr6g0309191 RchiOBHm_Chr6g0309201
rosa_laevigata RLG00000010582 RLG00000010583 RLG00000014937 RLG00000014938
rosa_multiflora Rmu_co8060540.1_g000001 Rmu_co8332663.1_g000001 Rmu_sc0000010.1_g000017 Rmu_sc0003711.1_g000008 Rmu_sc0016971.1_g000003 Rmu_sc0020102.1_g000006 Rmu_sc0020102.1_g000008
rosa_roxburghii Rroxscaffold_7G00159570 Rroxscaffold_7G00159580 Rroxscaffold_7G00159590 Rroxscaffold_7G00211100
rosa_rugosa Rorug05G0550400 Rorug06G0370100 Rorug06G0370300
rosa_samantha Rh6AG066900 Rh6AG483200 Rh6AG483300 Rh6AG483400 Rh6BG059800 Rh6BG060000 Rh6BG492200 Rh6BG492300 Rh6BG492500 Rh6CG059700 Rh6CG059800 Rh6CG496900 Rh6CG497000 Rh6CG497200 Rh6CG497400 Rh6DG056700 Rh6DG056800 Rh6DG483800 Rh6DG484000
rosa_wichuraiana Rw6G005860 Rw6G005870 Rw6G042070 Rw6G042080 Rw6G042090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 516
AccB1I GGYRCC 1 cut(s) 985
AccB7I CCANNNNNTGG 1 cut(s) 82
AccBSI CCGCTC 1 cut(s) 703
AccI GTMKAC 1 cut(s) 174
AccII CGCG 2 cut(s) 401, 749
AciI CCGC 9 cut(s) 54, 65, 159, 190, 200, 701, 758, 897, 1058
AclWI GGATC 2 cut(s) 44, 385
AcoI YGGCCR 1 cut(s) 197
AcsI RAATTY 1 cut(s) 406
AcuI CTGAAG 1 cut(s) 354
AcvI CACGTG 1 cut(s) 315
AfaI GTAC 2 cut(s) 93, 230
AfiI CCNNNNNNNGG 1 cut(s) 82
AflIII ACRYGT 1 cut(s) 747
AgsI TTSAA 3 cut(s) 382, 847, 1053
AjnI CCWGG 2 cut(s) 75, 87
AluBI AGCT 8 cut(s) 23, 32, 274, 283, 338, 791, 818, 889
AluI AGCT 8 cut(s) 23, 32, 274, 283, 338, 791, 818, 889
Alw21I GWGCWC 1 cut(s) 276
AlwI GGATC 2 cut(s) 44, 385
AoxI GGCC 7 cut(s) 84, 161, 183, 191, 197, 605, 947
ApeKI GCWGC 5 cut(s) 32, 412, 674, 697, 788
ApoI RAATTY 1 cut(s) 406
AspLEI GCGC 1 cut(s) 403
AspS9I GGNCC 5 cut(s) 85, 161, 184, 192, 947
AsuC2I CCSGG 5 cut(s) 156, 187, 196, 609, 714
AsuHPI GGTGA 2 cut(s) 580, 621
BaeGI GKGCMC 1 cut(s) 897
BanI GGYRCC 1 cut(s) 985
BanII GRGCYC 1 cut(s) 276
BauI CACGAG 1 cut(s) 908
BbrPI CACGTG 1 cut(s) 315
BbsI GAAGAC 1 cut(s) 510
Bbv12I GWGCWC 1 cut(s) 276
BbvI GCAGC 5 cut(s) 19, 424, 661, 709, 800
BccI CCATC 1 cut(s) 538
BceAI ACGGC 1 cut(s) 148
BcgI CGANNNNNNTGC 2 cut(s) 416, 450
BciT130I CCWGG 2 cut(s) 77, 89
BcnI CCSGG 5 cut(s) 156, 187, 196, 609, 714
BfaI CTAG 1 cut(s) 890
BfmI CTRYAG 1 cut(s) 24
BfuAI ACCTGC 1 cut(s) 516
BisI GCNGC 8 cut(s) 33, 191, 200, 413, 675, 698, 701, 789
BlpI GCTNAGC 1 cut(s) 876
BlsI GCNGC 8 cut(s) 34, 192, 201, 414, 676, 699, 702, 790
Bme1390I CCNGG 7 cut(s) 77, 89, 156, 187, 196, 609, 714
BmgT120I GGNCC 5 cut(s) 85, 161, 184, 192, 947
BmiI GGNNCC 4 cut(s) 194, 798, 987, 1106
BmrFI CCNGG 7 cut(s) 77, 89, 156, 187, 196, 609, 714
BmsI GCATC 3 cut(s) 49, 745, 837
BpiI GAAGAC 1 cut(s) 510
BpmI CTGGAG 1 cut(s) 921
Bpu10I CCTNAGC 1 cut(s) 704
Bpu1102I GCTNAGC 1 cut(s) 876
BpuMI CCSGG 5 cut(s) 156, 187, 196, 609, 714
BsaAI YACGTR 1 cut(s) 315
BsaJI CCNNGG 7 cut(s) 76, 87, 164, 194, 264, 512, 713
Bsc4I CCNNNNNNNGG 1 cut(s) 82
Bse1I ACTGG 4 cut(s) 109, 235, 950, 1019
Bse3DI GCAATG 1 cut(s) 379
BseBI CCWGG 2 cut(s) 77, 89
BseDI CCNNGG 7 cut(s) 76, 87, 164, 194, 264, 512, 713
BseGI GGATG 3 cut(s) 231, 844, 964
BseLI CCNNNNNNNGG 1 cut(s) 82
BseMI GCAATG 1 cut(s) 379
BseMII CTCAG 3 cut(s) 330, 718, 867
BseNI ACTGG 4 cut(s) 109, 235, 950, 1019
BseRI GAGGAG 3 cut(s) 578, 926, 941
BseSI GKGCMC 1 cut(s) 897
BseX3I CGGCCG 1 cut(s) 197
BseXI GCAGC 5 cut(s) 19, 424, 661, 709, 800
BseYI CCCAGC 1 cut(s) 279
Bsh1236I CGCG 2 cut(s) 401, 749
Bsh1285I CGRYCG 1 cut(s) 200
BshFI GGCC 7 cut(s) 86, 163, 185, 193, 199, 607, 949
BshNI GGYRCC 1 cut(s) 985
BsiEI CGRYCG 1 cut(s) 200
BsiHKAI GWGCWC 1 cut(s) 276
BsiSI CCGG 5 cut(s) 156, 187, 196, 608, 713
BslFI GGGAC 3 cut(s) 624, 670, 758
BslI CCNNNNNNNGG 1 cut(s) 82
BsmFI GGGAC 3 cut(s) 624, 670, 758
BsmI GAATGC 2 cut(s) 388, 652
BsnI GGCC 7 cut(s) 86, 163, 185, 193, 199, 607, 949
Bsp1286I GDGCHC 2 cut(s) 276, 897
Bsp143I GATC 2 cut(s) 49, 390
Bsp1720I GCTNAGC 1 cut(s) 876
BspACI CCGC 9 cut(s) 54, 65, 159, 190, 200, 701, 758, 897, 1058
BspANI GGCC 7 cut(s) 86, 163, 185, 193, 199, 607, 949
BspCNI CTCAG 3 cut(s) 331, 717, 868
BspFNI CGCG 2 cut(s) 401, 749
BspLI GGNNCC 4 cut(s) 194, 798, 987, 1106
BspMI ACCTGC 1 cut(s) 516
BspPI GGATC 2 cut(s) 44, 385
BspT107I GGYRCC 1 cut(s) 985
BsrBI CCGCTC 1 cut(s) 703
BsrDI GCAATG 1 cut(s) 379
BsrI ACTGG 4 cut(s) 109, 235, 950, 1019
BssECI CCNNGG 7 cut(s) 76, 87, 164, 194, 264, 512, 713
BssMI GATC 2 cut(s) 49, 390
BssSI CACGAG 1 cut(s) 908
Bst2BI CACGAG 1 cut(s) 908
Bst2UI CCWGG 2 cut(s) 77, 89
Bst4CI ACNGT 2 cut(s) 347, 1064
Bst6I CTCTTC 1 cut(s) 996
BstBAI YACGTR 1 cut(s) 315
BstC8I GCNNGC 3 cut(s) 161, 326, 897
BstDEI CTNAG 4 cut(s) 339, 448, 704, 876
BstDSI CCRYGG 1 cut(s) 164
BstF5I GGATG 3 cut(s) 231, 844, 964
BstFNI CGCG 2 cut(s) 401, 749
BstHHI GCGC 1 cut(s) 403
BstKTI GATC 2 cut(s) 52, 393
BstMBI GATC 2 cut(s) 49, 390
BstMCI CGRYCG 1 cut(s) 200
BstMWI GCNNNNNNNGC 8 cut(s) 29, 196, 199, 280, 409, 694, 755, 886
BstNI CCWGG 2 cut(s) 77, 89
BstSCI CCNGG 7 cut(s) 75, 87, 154, 185, 194, 607, 712
BstSFI CTRYAG 1 cut(s) 24
BstSLI GKGCMC 1 cut(s) 897
BstUI CGCG 2 cut(s) 401, 749
BstV1I GCAGC 5 cut(s) 19, 424, 661, 709, 800
BstV2I GAAGAC 1 cut(s) 510
BstZI CGGCCG 1 cut(s) 197
BsuRI GGCC 7 cut(s) 86, 163, 185, 193, 199, 607, 949
BtgI CCRYGG 1 cut(s) 164
BtsCI GGATG 3 cut(s) 231, 844, 964
BtsI GCAGTG 2 cut(s) 140, 209
BtsIMutI CAGTG 5 cut(s) 140, 209, 242, 285, 957
BveI ACCTGC 1 cut(s) 516
Cac8I GCNNGC 3 cut(s) 161, 326, 897
CfoI GCGC 1 cut(s) 403
Cfr13I GGNCC 5 cut(s) 85, 161, 184, 192, 947
CseI GACGC 2 cut(s) 738, 755
Csp6I GTAC 2 cut(s) 92, 229
CviAII CATG 7 cut(s) 485, 495, 591, 689, 776, 977, 1085
CviQI GTAC 2 cut(s) 92, 229
DdeI CTNAG 4 cut(s) 339, 448, 704, 876
DpnI GATC 2 cut(s) 51, 392
DpnII GATC 2 cut(s) 49, 390
EaeI YGGCCR 1 cut(s) 197
EagI CGGCCG 1 cut(s) 197
Eam1104I CTCTTC 1 cut(s) 996
EarI CTCTTC 1 cut(s) 996
Ecl136II GAGCTC 1 cut(s) 274
EclXI CGGCCG 1 cut(s) 197
Eco24I GRGCYC 1 cut(s) 276
Eco52I CGGCCG 1 cut(s) 197
Eco53kI GAGCTC 1 cut(s) 274
Eco57I CTGAAG 1 cut(s) 354
Eco72I CACGTG 1 cut(s) 315
EcoICRI GAGCTC 1 cut(s) 274
EcoRII CCWGG 2 cut(s) 75, 87
EcoT38I GRGCYC 1 cut(s) 276
FaeI CATG 7 cut(s) 488, 498, 594, 692, 779, 980, 1088
FalI AAGNNNNNCTT 2 cut(s) 431, 463
FaqI GGGAC 3 cut(s) 624, 670, 758
FatI CATG 7 cut(s) 484, 494, 590, 688, 775, 976, 1084
FauI CCCGC 3 cut(s) 61, 152, 904
FauNDI CATATG 1 cut(s) 419
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 8 cut(s) 33, 191, 200, 413, 675, 698, 701, 789
FokI GGATG 3 cut(s) 218, 851, 971
FriOI GRGCYC 1 cut(s) 276
Fsp4HI GCNGC 8 cut(s) 33, 191, 200, 413, 675, 698, 701, 789
FspBI CTAG 1 cut(s) 890
GlaI GCGC 1 cut(s) 402
GluI GCNGC 8 cut(s) 33, 191, 200, 413, 675, 698, 701, 789
GsaI CCCAGC 1 cut(s) 283
GsuI CTGGAG 1 cut(s) 921
HaeIII GGCC 7 cut(s) 86, 163, 185, 193, 199, 607, 949
HapII CCGG 5 cut(s) 156, 187, 196, 608, 713
HgaI GACGC 2 cut(s) 738, 755
HhaI GCGC 1 cut(s) 403
Hin1II CATG 7 cut(s) 488, 498, 594, 692, 779, 980, 1088
Hin6I GCGC 1 cut(s) 401
HinP1I GCGC 1 cut(s) 401
HindIII AAGCTT 1 cut(s) 816
HinfI GANTC 8 cut(s) 108, 133, 223, 251, 612, 738, 868, 1049
HpaII CCGG 5 cut(s) 156, 187, 196, 608, 713
HphI GGTGA 2 cut(s) 580, 621
Hpy166II GTNNAC 3 cut(s) 175, 318, 907
Hpy188I TCNGA 4 cut(s) 116, 250, 438, 754
Hpy188III TCNNGA 3 cut(s) 500, 908, 1025
Hpy8I GTNNAC 3 cut(s) 175, 318, 907
Hpy99I CGWCG 2 cut(s) 116, 1044
HpyAV CCTTC 4 cut(s) 163, 571, 686, 728
HpyCH4III ACNGT 2 cut(s) 347, 1064
HpyCH4IV ACGT 1 cut(s) 314
HpyCH4V TGCA 6 cut(s) 35, 62, 412, 427, 601, 828
HpyF10VI GCNNNNNNNGC 8 cut(s) 29, 196, 199, 280, 409, 694, 755, 886
HpyF3I CTNAG 4 cut(s) 339, 448, 704, 876
HpySE526I ACGT 1 cut(s) 314
Hsp92II CATG 7 cut(s) 488, 498, 594, 692, 779, 980, 1088
HspAI GCGC 1 cut(s) 401
Kzo9I GATC 2 cut(s) 49, 390
LmnI GCTCC 2 cut(s) 29, 796
Lsp1109I GCAGC 5 cut(s) 19, 424, 661, 709, 800
LweI GCATC 3 cut(s) 49, 745, 837
MaeI CTAG 1 cut(s) 890
MaeII ACGT 1 cut(s) 314
MaeIII GTNAC 5 cut(s) 100, 239, 292, 310, 497
MalI GATC 2 cut(s) 51, 392
MbiI CCGCTC 1 cut(s) 703
MboI GATC 2 cut(s) 49, 390
MboII GAAGA 5 cut(s) 128, 515, 583, 586, 1013
MhlI GDGCHC 2 cut(s) 276, 897
MluCI AATT 6 cut(s) 406, 529, 823, 963, 995, 1155
MluI ACGCGT 1 cut(s) 747
MlyI GAGTC 3 cut(s) 102, 245, 606
MmeI TCCRAC 3 cut(s) 94, 416, 502
MseI TTAA 2 cut(s) 519, 822
MslI CAYNNNNRTG 1 cut(s) 422
MspA1I CMGCKG 2 cut(s) 791, 899
MspI CCGG 5 cut(s) 156, 187, 196, 608, 713
MspR9I CCNGG 7 cut(s) 77, 89, 156, 187, 196, 609, 714
Mva1269I GAATGC 2 cut(s) 388, 652
MvaI CCWGG 2 cut(s) 77, 89
MvnI CGCG 2 cut(s) 401, 749
MwoI GCNNNNNNNGC 8 cut(s) 29, 196, 199, 280, 409, 694, 755, 886
NciI CCSGG 5 cut(s) 156, 187, 196, 609, 714
NdeI CATATG 1 cut(s) 419
NdeII GATC 2 cut(s) 49, 390
NlaIII CATG 7 cut(s) 488, 498, 594, 692, 779, 980, 1088
NlaIV GGNNCC 4 cut(s) 194, 798, 987, 1106
NmeAIII GCCGAG 2 cut(s) 154, 537
NmuCI GTSAC 4 cut(s) 239, 292, 310, 497
PctI GAATGC 2 cut(s) 388, 652
PfeI GAWTC 5 cut(s) 133, 223, 738, 868, 1049
PflMI CCANNNNNTGG 1 cut(s) 82
PkrI GCNGC 8 cut(s) 34, 192, 201, 414, 676, 699, 702, 790
PleI GAGTC 3 cut(s) 102, 245, 606
PmaCI CACGTG 1 cut(s) 315
PmlI CACGTG 1 cut(s) 315
PpsI GAGTC 3 cut(s) 102, 245, 606
Ppu21I YACGTR 1 cut(s) 315
Psp124BI GAGCTC 1 cut(s) 276
Psp6I CCWGG 2 cut(s) 75, 87
PspCI CACGTG 1 cut(s) 315
PspFI CCCAGC 1 cut(s) 279
PspGI CCWGG 2 cut(s) 75, 87
PspN4I GGNNCC 4 cut(s) 194, 798, 987, 1106
PspPI GGNCC 5 cut(s) 85, 161, 184, 192, 947
PvuII CAGCTG 1 cut(s) 791
RsaI GTAC 2 cut(s) 93, 230
RsaNI GTAC 2 cut(s) 92, 229
RseI CAYNNNNRTG 1 cut(s) 422
SacI GAGCTC 1 cut(s) 276
SaqAI TTAA 2 cut(s) 519, 822
SatI GCNGC 8 cut(s) 33, 191, 200, 413, 675, 698, 701, 789
Sau3AI GATC 2 cut(s) 49, 390
Sau96I GGNCC 5 cut(s) 85, 161, 184, 192, 947
SchI GAGTC 3 cut(s) 102, 245, 606
ScrFI CCNGG 7 cut(s) 77, 89, 156, 187, 196, 609, 714
SduI GDGCHC 2 cut(s) 276, 897
SfaNI GCATC 3 cut(s) 49, 745, 837
SfcI CTRYAG 1 cut(s) 24
SmiMI CAYNNNNRTG 1 cut(s) 422
Sse9I AATT 6 cut(s) 406, 529, 823, 963, 995, 1155
SsiI CCGC 9 cut(s) 54, 65, 159, 190, 200, 701, 758, 897, 1058
SspMI CTAG 1 cut(s) 890
SstI GAGCTC 1 cut(s) 276
StyD4I CCNGG 7 cut(s) 75, 87, 154, 185, 194, 607, 712
TaaI ACNGT 2 cut(s) 347, 1064
TaiI ACGT 1 cut(s) 317
TaqI TCGA 2 cut(s) 885, 1039
TaqII GACCGA 1 cut(s) 278
TasI AATT 6 cut(s) 406, 529, 823, 963, 995, 1155
TatI WGTACW 1 cut(s) 228
TauI GCSGC 3 cut(s) 193, 202, 703
TfiI GAWTC 5 cut(s) 133, 223, 738, 868, 1049
Tru1I TTAA 2 cut(s) 519, 822
Tru9I TTAA 2 cut(s) 519, 822
TscAI CASTG 5 cut(s) 147, 209, 242, 292, 957
TseFI GTSAC 4 cut(s) 239, 292, 310, 497
TseI GCWGC 5 cut(s) 32, 412, 674, 697, 788
Tsp45I GTSAC 4 cut(s) 239, 292, 310, 497
TspDTI ATGAA 8 cut(s) 501, 659, 705, 828, 950, 975, 1082, 1157
TspGWI ACGGA 2 cut(s) 192, 1057
TspRI CASTG 5 cut(s) 147, 209, 242, 292, 957
Van91I CCANNNNNTGG 1 cut(s) 82
XapI RAATTY 1 cut(s) 406
XmiI GTMKAC 1 cut(s) 174
XspI CTAG 1 cut(s) 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.