Prupe.1G426100_v2.0.a1

3-oxo-Delta(4,5)-steroid 5-beta-reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000626
Physical Location & Seq
Forward (+)
36645103 .. 36649762
4660 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G426100.2

Sequence Viewer

Length: 1176 bp
ATGAGCTGGTGGTGGGCTGGAGCTGTTGGCGCTGCCAAGAAAAAATTTGACGAAGACGAAGCACCGCGAAGCCCCGAGAGCGTAGGTCTCGTGATCGGCGTCACCGGCATAGTCGGCAACAGCCTCGCCGAAATCCTCCCGATCTCCGACACCCCCGGGGGTCCATGGAAGGTCTACGGCGTCGCACGCCGTCCCCGCCCGAACTGGAACGCCGACCACCCCGTCGAGTACATCCAGTGCGACATCTCCGATGCCGACGACGCCAAGGCCAAGCTCTCTCCGTTATCCGACGTCACCCACATCTTCTACGTGACCTGGACCAACCGATCCACCGAGGCCGAGAACTGCGAGGCTAACGGCGCTATGTTCCGCAACGTCTTGAATTCCGTCATCCCAAACGCACCCAATCTCCGCCACGTGTGCCTCCAGACCGGGGCCAAGCACTACGTCGGGCCGTTCGAGGCGTTCGGAAAGATCCAGCCCCACGAGCCGCCCTTCACGGAGGACCTGCCCCGATTGGACTACCCGAATTTCTACTACACCATGGAAGATTTGTTGTTCGCTGAGATGGAGAAGAAGGAGGACTTGACTTGGTCCGTCCACCGACCCGATGCGATTTTCGGGTTCTCTCCGTACAGCATGATGAACATCGTCGGCACGCTCTGCGTGTACGCCGCCATCTGCAAGCACGAGAGAGTGCCGTTAAAGTTCCCGGGAAGCAAAGCGGCGTGGAACTGCTACTCGGTGGCTTCGGATGCGGATCTGATAGCGGAGCAGCACATATGGGCGGCGGTGGACCCGTATGCCAAAAACGAAGCGTTTAACATAAACAATGGAGATGTGTTTAAGTGGAAGCATTTCTGGAAGGTGTTGGCTGAGCAATTTGGGATTGAGGAGTATGGGATTGATGAGGAGGAGGGGGCGAAGCTGAGCTTGGTGGAGCTGATGAAGGGGAAGGAGAGAGTGTGGGAGGAGATAGTGAAGGAGAATCAGCTGCAGCCCACAAGGTTGGAGGAGGTTGGGGTGTGGTGGTTTGTGGATGCTTGGTTGGGTGGGGAGGGATTGTTGTGTAGTATGAACAAGAGCAAGGAGCATGGGTTTGTGGGTTTTAGAAACTCCAGGAATTCATTTGTCACTTGGATTGATAAAACGAAGGCTTTCAAGATTGTGCCTTGA

Protein Analysis

392

Amino Acids

44.23

Weight (kDa)

5.26

Isoelectric Point (pI)

35.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000626)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24220 AT4G24220
fragaria_vesca FvH4_2g04580 FvH4_2g04580 FvH4_2g04580 FvH4_4g06071 FvH4_5g25130
malus_domestica MD05G1094500.v1.1 MD05G1108300.v1.1 MD08G1088400.v1.1 MD15G1073200.v1.1 MD15G1073300.v1.1 MD15G1073400.v1.1 MD15G1073500.v1.1 MD15G1073600.v1.1
prunus_persica Prupe.1G426100_v2.0.a1 Prupe.1G426100_v2.0.a1 Prupe.1G426200_v2.0.a1 Prupe.1G426300_v2.0.a1 Prupe.1G426400_v2.0.a1 Prupe.1G426400_v2.0.a1 Prupe.8G139400_v2.0.a1 Prupe.8G139400_v2.0.a1
pyrus_communis pycom05g09350 pycom08g07190 pycom13g22780 pycom15g06840 pycom15g06850 pycom15g06870
rosa_chinensis RchiOBHm_Chr6g0254161 RchiOBHm_Chr6g0254181 RchiOBHm_Chr6g0254191 RchiOBHm_Chr6g0309171 RchiOBHm_Chr6g0309181 RchiOBHm_Chr6g0309191 RchiOBHm_Chr6g0309201
rosa_laevigata RLG00000010582 RLG00000010583 RLG00000014937 RLG00000014938
rosa_multiflora Rmu_co8060540.1_g000001 Rmu_co8332663.1_g000001 Rmu_sc0000010.1_g000017 Rmu_sc0003711.1_g000008 Rmu_sc0016971.1_g000003 Rmu_sc0020102.1_g000006 Rmu_sc0020102.1_g000008
rosa_roxburghii Rroxscaffold_7G00159570 Rroxscaffold_7G00159580 Rroxscaffold_7G00159590 Rroxscaffold_7G00211100
rosa_rugosa Rorug05G0550400 Rorug06G0370100 Rorug06G0370300
rosa_samantha Rh6AG066900 Rh6AG483200 Rh6AG483300 Rh6AG483400 Rh6BG059800 Rh6BG060000 Rh6BG492200 Rh6BG492300 Rh6BG492500 Rh6CG059700 Rh6CG059800 Rh6CG496900 Rh6CG497000 Rh6CG497200 Rh6CG497400 Rh6DG056700 Rh6DG056800 Rh6DG483800 Rh6DG484000
rosa_wichuraiana Rw6G005860 Rw6G005870 Rw6G042070 Rw6G042080 Rw6G042090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 221
AatII GACGTC 1 cut(s) 294
Acc36I ACCTGC 1 cut(s) 516
AccI GTMKAC 1 cut(s) 174
AccII CGCG 1 cut(s) 67
AclWI GGATC 3 cut(s) 321, 469, 768
AcsI RAATTY 4 cut(s) 44, 382, 529, 1123
AcvI CACGTG 1 cut(s) 418
AcyI GRCGYC 4 cut(s) 99, 180, 261, 291
AfaI GTAC 3 cut(s) 230, 635, 671
AfiI CCNNNNNNNGG 1 cut(s) 433
AflIII ACRYGT 1 cut(s) 417
AgsI TTSAA 2 cut(s) 382, 1162
AjnI CCWGG 2 cut(s) 314, 1118
AjuI GAANNNNNNNTTGG 2 cut(s) 917, 949
AluBI AGCT 7 cut(s) 6, 23, 274, 928, 933, 943, 994
AluI AGCT 7 cut(s) 6, 23, 274, 928, 933, 943, 994
Alw26I GTCTC 1 cut(s) 92
AlwI GGATC 3 cut(s) 321, 469, 768
Ama87I CYCGRG 3 cut(s) 74, 155, 712
AoxI GGCC 4 cut(s) 267, 336, 435, 452
ApeKI GCWGC 4 cut(s) 32, 775, 994, 997
ApoI RAATTY 4 cut(s) 44, 382, 529, 1123
Asp700I GAANNNNTTC 2 cut(s) 857, 1157
AspLEI GCGC 2 cut(s) 32, 362
AspS9I GGNCC 7 cut(s) 161, 318, 435, 452, 505, 594, 796
AsuC2I CCSGG 5 cut(s) 156, 157, 433, 713, 714
AsuHPI GGTGA 2 cut(s) 94, 286
AvaI CYCGRG 3 cut(s) 74, 155, 712
AvaII GGWCC 5 cut(s) 161, 318, 505, 594, 796
BauI CACGAG 3 cut(s) 89, 485, 689
BbrPI CACGTG 1 cut(s) 418
BbsI GAAGAC 1 cut(s) 60
BbvI GCAGC 4 cut(s) 19, 787, 981, 1009
BccI CCATC 2 cut(s) 562, 686
BceAI ACGGC 5 cut(s) 174, 193, 373, 439, 685
BcgI CGANNNNNNTGC 2 cut(s) 106, 140
BciT130I CCWGG 2 cut(s) 316, 1120
BcnI CCSGG 5 cut(s) 156, 157, 433, 713, 714
BcoDI GTCTC 1 cut(s) 92
BfmI CTRYAG 1 cut(s) 995
BfoI RGCGCY 2 cut(s) 33, 363
BfuAI ACCTGC 1 cut(s) 516
BisI GCNGC 8 cut(s) 33, 491, 675, 726, 776, 789, 995, 998
BlpI GCTNAGC 2 cut(s) 876, 929
BlsI GCNGC 8 cut(s) 34, 492, 676, 727, 777, 790, 996, 999
Bme1390I CCNGG 7 cut(s) 156, 157, 316, 433, 713, 714, 1120
Bme18I GGWCC 5 cut(s) 161, 318, 505, 594, 796
BmeT110I CYCGRG 3 cut(s) 74, 155, 712
BmgT120I GGNCC 7 cut(s) 161, 318, 435, 452, 505, 594, 796
BmiI GGNNCC 3 cut(s) 162, 436, 798
BmrFI CCNGG 7 cut(s) 156, 157, 316, 433, 713, 714, 1120
BmsI GCATC 4 cut(s) 241, 601, 745, 1030
BpiI GAAGAC 1 cut(s) 60
BpmI CTGGAG 3 cut(s) 39, 410, 1102
Bpu1102I GCTNAGC 2 cut(s) 876, 929
BpuMI CCSGG 5 cut(s) 156, 157, 433, 713, 714
BsaAI YACGTR 2 cut(s) 310, 418
BsaBI GATNNNNATC 2 cut(s) 647, 759
BsaHI GRCGYC 4 cut(s) 99, 180, 261, 291
BsaI GGTCTC 1 cut(s) 92
BsaJI CCNNGG 9 cut(s) 154, 155, 156, 164, 264, 333, 432, 543, 712
BsaXI ACNNNNNCTCC 8 cut(s) 393, 423, 950, 980, 1049, 1079, 1082, 1112
Bsc4I CCNNNNNNNGG 1 cut(s) 433
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 2 cut(s) 209, 235
Bse8I GATNNNNATC 2 cut(s) 647, 759
BseBI CCWGG 2 cut(s) 316, 1120
BseDI CCNNGG 9 cut(s) 154, 155, 156, 164, 264, 333, 432, 543, 712
BseGI GGATG 4 cut(s) 231, 390, 760, 1045
BseJI GATNNNNATC 2 cut(s) 647, 759
BseLI CCNNNNNNNGG 1 cut(s) 433
BseMII CTCAG 3 cut(s) 555, 867, 920
BseNI ACTGG 2 cut(s) 209, 235
BseRI GAGGAG 5 cut(s) 908, 926, 929, 986, 1028
BseXI GCAGC 4 cut(s) 19, 787, 981, 1009
Bsh1236I CGCG 1 cut(s) 67
BshFI GGCC 4 cut(s) 269, 338, 437, 454
BsiHKCI CYCGRG 3 cut(s) 74, 155, 712
BsiSI CCGG 4 cut(s) 105, 156, 432, 713
BslFI GGGAC 1 cut(s) 177
BslI CCNNNNNNNGG 1 cut(s) 433
BsmAI GTCTC 1 cut(s) 92
BsmFI GGGAC 1 cut(s) 177
BsnI GGCC 4 cut(s) 269, 338, 437, 454
Bso31I GGTCTC 1 cut(s) 92
BsoBI CYCGRG 3 cut(s) 74, 155, 712
Bsp143I GATC 5 cut(s) 93, 141, 326, 474, 760
Bsp1720I GCTNAGC 2 cut(s) 876, 929
Bsp19I CCATGG 2 cut(s) 164, 543
BspANI GGCC 4 cut(s) 269, 338, 437, 454
BspCNI CTCAG 3 cut(s) 556, 868, 921
BspFNI CGCG 1 cut(s) 67
BspLI GGNNCC 3 cut(s) 162, 436, 798
BspMAI CTGCAG 1 cut(s) 999
BspMI ACCTGC 1 cut(s) 516
BspPI GGATC 3 cut(s) 321, 469, 768
BspTNI GGTCTC 1 cut(s) 92
BsrFI RCCGGY 1 cut(s) 104
BsrI ACTGG 2 cut(s) 209, 235
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 9 cut(s) 154, 155, 156, 164, 264, 333, 432, 543, 712
BssMI GATC 5 cut(s) 93, 141, 326, 474, 760
BssNI GRCGYC 4 cut(s) 99, 180, 261, 291
BssSI CACGAG 3 cut(s) 89, 485, 689
BssT1I CCWWGG 3 cut(s) 164, 264, 543
Bst2BI CACGAG 3 cut(s) 89, 485, 689
Bst2UI CCWGG 2 cut(s) 316, 1120
BstACI GRCGYC 4 cut(s) 99, 180, 261, 291
BstAPI GCANNNNNTGC 1 cut(s) 663
BstBAI YACGTR 2 cut(s) 310, 418
BstC8I GCNNGC 3 cut(s) 187, 659, 686
BstDEI CTNAG 3 cut(s) 564, 876, 929
BstDSI CCRYGG 2 cut(s) 164, 543
BstF5I GGATG 4 cut(s) 231, 390, 760, 1045
BstFNI CGCG 1 cut(s) 67
BstH2I RGCGCY 2 cut(s) 33, 363
BstHHI GCGC 2 cut(s) 32, 362
BstKTI GATC 5 cut(s) 96, 144, 329, 477, 763
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 5 cut(s) 93, 141, 326, 474, 760
BstNI CCWGG 2 cut(s) 316, 1120
BstSCI CCNGG 7 cut(s) 154, 155, 314, 431, 711, 712, 1118
BstSFI CTRYAG 1 cut(s) 995
BstUI CGCG 1 cut(s) 67
BstV1I GCAGC 4 cut(s) 19, 787, 981, 1009
BstV2I GAAGAC 1 cut(s) 60
BstX2I RGATCY 2 cut(s) 474, 760
BstXI CCANNNNNNTGG 1 cut(s) 1009
BstYI RGATCY 2 cut(s) 474, 760
BsuRI GGCC 4 cut(s) 269, 338, 437, 454
BtgI CCRYGG 2 cut(s) 164, 543
BtsCI GGATG 4 cut(s) 231, 390, 760, 1045
BtsIMutI CAGTG 1 cut(s) 242
BveI ACCTGC 1 cut(s) 516
Cac8I GCNNGC 3 cut(s) 187, 659, 686
CfoI GCGC 2 cut(s) 32, 362
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 7 cut(s) 161, 318, 435, 452, 505, 594, 796
Cfr9I CCCGGG 2 cut(s) 155, 712
CseI GACGC 3 cut(s) 88, 169, 269
Csp6I GTAC 3 cut(s) 229, 634, 670
CviAII CATG 4 cut(s) 165, 544, 640, 1094
CviQI GTAC 3 cut(s) 229, 634, 670
DdeI CTNAG 3 cut(s) 564, 876, 929
DpnI GATC 5 cut(s) 95, 143, 328, 476, 762
DpnII GATC 5 cut(s) 93, 141, 326, 474, 760
DrdI GACNNNNNNGTC 1 cut(s) 221
DseDI GACNNNNNNGTC 1 cut(s) 221
EciI GGCGGA 1 cut(s) 401
Eco130I CCWWGG 3 cut(s) 164, 264, 543
Eco31I GGTCTC 1 cut(s) 92
Eco47I GGWCC 5 cut(s) 161, 318, 505, 594, 796
Eco72I CACGTG 1 cut(s) 418
Eco88I CYCGRG 3 cut(s) 74, 155, 712
EcoO109I RGGNCCY 1 cut(s) 505
EcoRI GAATTC 2 cut(s) 382, 1123
EcoRII CCWGG 2 cut(s) 314, 1118
EcoT14I CCWWGG 3 cut(s) 164, 264, 543
ErhI CCWWGG 3 cut(s) 164, 264, 543
FaeI CATG 4 cut(s) 168, 547, 643, 1097
FalI AAGNNNNNCTT 4 cut(s) 569, 601, 917, 949
FaqI GGGAC 1 cut(s) 177
FatI CATG 4 cut(s) 164, 543, 639, 1093
FauI CCCGC 1 cut(s) 203
FauNDI CATATG 1 cut(s) 782
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 8 cut(s) 33, 491, 675, 726, 776, 789, 995, 998
FokI GGATG 4 cut(s) 218, 377, 767, 1052
Fsp4HI GCNGC 8 cut(s) 33, 491, 675, 726, 776, 789, 995, 998
GlaI GCGC 2 cut(s) 31, 361
GluI GCNGC 8 cut(s) 33, 491, 675, 726, 776, 789, 995, 998
GsuI CTGGAG 3 cut(s) 39, 410, 1102
HaeII RGCGCY 2 cut(s) 33, 363
HaeIII GGCC 4 cut(s) 269, 338, 437, 454
HapII CCGG 4 cut(s) 105, 156, 432, 713
HgaI GACGC 3 cut(s) 88, 169, 269
HhaI GCGC 2 cut(s) 32, 362
Hin1I GRCGYC 4 cut(s) 99, 180, 261, 291
Hin1II CATG 4 cut(s) 168, 547, 643, 1097
Hin6I GCGC 2 cut(s) 30, 360
HinP1I GCGC 2 cut(s) 30, 360
HinfI GANTC 1 cut(s) 988
HpaII CCGG 4 cut(s) 105, 156, 432, 713
HphI GGTGA 2 cut(s) 94, 286
Hpy166II GTNNAC 4 cut(s) 175, 601, 670, 796
Hpy188I TCNGA 6 cut(s) 148, 250, 289, 470, 754, 765
Hpy188III TCNNGA 6 cut(s) 91, 139, 379, 427, 862, 1162
Hpy8I GTNNAC 4 cut(s) 175, 601, 670, 796
Hpy99I CGWCG 7 cut(s) 185, 227, 260, 263, 293, 452, 656
HpyAV CCTTC 8 cut(s) 163, 505, 571, 859, 943, 949, 976, 1147
HpyCH4IV ACGT 5 cut(s) 291, 309, 375, 417, 447
HpyCH4V TGCA 2 cut(s) 684, 997
HpyF3I CTNAG 3 cut(s) 564, 876, 929
HpySE526I ACGT 5 cut(s) 291, 309, 375, 417, 447
Hsp92I GRCGYC 4 cut(s) 99, 180, 261, 291
Hsp92II CATG 4 cut(s) 168, 547, 643, 1097
HspAI GCGC 2 cut(s) 30, 360
Kzo9I GATC 5 cut(s) 93, 141, 326, 474, 760
LmnI GCTCC 4 cut(s) 20, 772, 940, 1090
Lsp1109I GCAGC 4 cut(s) 19, 787, 981, 1009
LweI GCATC 4 cut(s) 241, 601, 745, 1030
MaeII ACGT 5 cut(s) 291, 309, 375, 417, 447
MaeIII GTNAC 4 cut(s) 100, 292, 310, 1132
MalI GATC 5 cut(s) 95, 143, 328, 476, 762
MboI GATC 5 cut(s) 93, 141, 326, 474, 760
MboII GAAGA 4 cut(s) 65, 295, 560, 586
MflI RGATCY 2 cut(s) 474, 760
MluCI AATT 5 cut(s) 44, 382, 529, 881, 1123
MmeI TCCRAC 3 cut(s) 171, 312, 990
MroXI GAANNNNTTC 2 cut(s) 857, 1157
MseI TTAA 3 cut(s) 704, 822, 846
MspA1I CMGCKG 1 cut(s) 994
MspI CCGG 4 cut(s) 105, 156, 432, 713
MspR9I CCNGG 7 cut(s) 156, 157, 316, 433, 713, 714, 1120
MvaI CCWGG 2 cut(s) 316, 1120
MvnI CGCG 1 cut(s) 67
NciI CCSGG 5 cut(s) 156, 157, 433, 713, 714
NcoI CCATGG 2 cut(s) 164, 543
NdeI CATATG 1 cut(s) 782
NdeII GATC 5 cut(s) 93, 141, 326, 474, 760
NlaIII CATG 4 cut(s) 168, 547, 643, 1097
NlaIV GGNNCC 3 cut(s) 162, 436, 798
NmeAIII GCCGAG 1 cut(s) 364
NmuCI GTSAC 4 cut(s) 100, 292, 310, 1132
PcsI WCGNNNNNNNCGW 2 cut(s) 96, 456
PdmI GAANNNNTTC 2 cut(s) 857, 1157
PfeI GAWTC 1 cut(s) 988
PflFI GACNNNGTC 1 cut(s) 592
PfoI TCCNGGA 1 cut(s) 1118
PkrI GCNGC 8 cut(s) 34, 492, 676, 727, 777, 790, 996, 999
PmaCI CACGTG 1 cut(s) 418
PmlI CACGTG 1 cut(s) 418
Ppu21I YACGTR 2 cut(s) 310, 418
PpuMI RGGWCCY 1 cut(s) 505
Psp5II RGGWCCY 1 cut(s) 505
Psp6I CCWGG 2 cut(s) 314, 1118
PspCI CACGTG 1 cut(s) 418
PspGI CCWGG 2 cut(s) 314, 1118
PspN4I GGNNCC 3 cut(s) 162, 436, 798
PspPI GGNCC 7 cut(s) 161, 318, 435, 452, 505, 594, 796
PspPPI RGGWCCY 1 cut(s) 505
PstI CTGCAG 1 cut(s) 999
PsuI RGATCY 2 cut(s) 474, 760
PsyI GACNNNGTC 1 cut(s) 592
PvuII CAGCTG 1 cut(s) 994
RsaI GTAC 3 cut(s) 230, 635, 671
RsaNI GTAC 3 cut(s) 229, 634, 670
SaqAI TTAA 3 cut(s) 704, 822, 846
SatI GCNGC 8 cut(s) 33, 491, 675, 726, 776, 789, 995, 998
Sau3AI GATC 5 cut(s) 93, 141, 326, 474, 760
Sau96I GGNCC 7 cut(s) 161, 318, 435, 452, 505, 594, 796
ScrFI CCNGG 7 cut(s) 156, 157, 316, 433, 713, 714, 1120
SfaNI GCATC 4 cut(s) 241, 601, 745, 1030
SfcI CTRYAG 1 cut(s) 995
SinI GGWCC 5 cut(s) 161, 318, 505, 594, 796
SmaI CCCGGG 2 cut(s) 157, 714
Sse9I AATT 5 cut(s) 44, 382, 529, 881, 1123
StyD4I CCNGG 7 cut(s) 154, 155, 314, 431, 711, 712, 1118
StyI CCWWGG 3 cut(s) 164, 264, 543
TaiI ACGT 5 cut(s) 294, 312, 378, 420, 450
TaqI TCGA 2 cut(s) 225, 459
TasI AATT 5 cut(s) 44, 382, 529, 881, 1123
TatI WGTACW 1 cut(s) 228
TauI GCSGC 4 cut(s) 493, 677, 728, 791
TfiI GAWTC 1 cut(s) 988
Tru1I TTAA 3 cut(s) 704, 822, 846
Tru9I TTAA 3 cut(s) 704, 822, 846
TscAI CASTG 1 cut(s) 242
TseFI GTSAC 4 cut(s) 100, 292, 310, 1132
TseI GCWGC 4 cut(s) 32, 775, 994, 997
Tsp45I GTSAC 4 cut(s) 100, 292, 310, 1132
TspDTI ATGAA 4 cut(s) 659, 962, 1091, 1116
TspGWI ACGGA 5 cut(s) 270, 376, 515, 586, 621
TspMI CCCGGG 2 cut(s) 155, 712
TspRI CASTG 1 cut(s) 242
Tth111I GACNNNGTC 1 cut(s) 592
VpaK11BI GGWCC 5 cut(s) 161, 318, 505, 594, 796
XapI RAATTY 4 cut(s) 44, 382, 529, 1123
XmaI CCCGGG 2 cut(s) 155, 712
XmiI GTMKAC 1 cut(s) 174
XmnI GAANNNNTTC 2 cut(s) 857, 1157
ZraI GACGTC 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.