Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0000626

Physical Location & Seq

Genomic Coordinates Forward (+)

5005605 .. 5007209

Gene Structure 1605 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1073500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1179 bp

ATGAGCTGGTGGTGGGCAAGTGCTGTTGAAGCTGCCAAGAAGAAACACCAAGAAGATCGTTACGAACCACCAAGTATCCCCAAGAGCGTAGGGTTGGTGATCGGCATAACGGGTATAGTGGGCAACAGCCTCGCCGAAATCCTCCCGCTCTCCGACACTCCTGGCGGTCCATGGAAGGTTTATGGAGTTGCTAGGCGTCCGCGCCCGCACTGGAACGCAGACCACGCAGTGGACTATATACAGTGCGACATCTCAGACCCGGACGACACCCAAAAAAAGCTCTCCAAATTAAGTGACGTTACCCACATCTTCTACGTTACTTGGACTAACCGGTCCACTGAGAACGAAAATTGCGAGGCCAATGCCGCCATGTTGCGCAACGTTCTGCGCATCGTTATTTCAAACGCGCCGAATCTCCGTCACGTGTGCTTGCAAACAGGAACCAAACAATACATCGGACCATTCGAGACGTTTGGGAAAATTGATCATCATGACCCGCCTTGTTCGGAGGATCTGCCGCGATTGAACGCGCCTAATTTCTATTATGCCCTCGAGGACGTGTTACTGGAAGAGGTAGGGAAGAAGGAGGATATCACTTGGTCCGTCCATAGGCCCGGGGTTATTTTCGGGTTTTCGCCTTATAGTTTAGCGAACATGGTTGCCATGCTGAGTGTGTACGCGGCAATATGCAAGCACGAAGGGATGCCCTTGAAATTTCCCGGAAGCAAAGAGGCTTGGAACTGCTACTCTGATGCTTCCGATGCGGATCTGATAGCGGAGCAGCAGATATGGGCGGCGGTGGACCCATACGCCAAAAACAAAGCGTTTAATGTCAACAATGGGGATGTTTTCAAGTGGAAGCAGTTATGGAAGGTGTTGGCTGAGCAGTTTGAGATTGAGGAGTACGGGTTTGATGAGGAGGGTGAGAAGCTGAGCTTGGCGGAGTTGATGAAGGTGAAAGAGGGGGTGTGGGAGCAGATAGTGAAGGAAAATCAGCTGGAGCCGATGAAGTTGGTGGAGATCGGACAGTGGTGGTTGGTGGATCTTGTGTTCGGTGGGGAAGGCTTGATGACTAGTATGAACAAGAGTAAGGAACATGGATTTTTGGGGTTTAGAAACTCTGGGAATTCGTTTGTCAGTTGGATTGATAAACTGAAAGCTTTTAAGATTGTGCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003002 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006629 GO:0006950 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0008202 GO:0009611 GO:0010051 GO:0016491 GO:0016627 GO:0016628 GO:0032501 GO:0032502 GO:0035671 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0048856 GO:0050896 GO:0055114 GO:0071704 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Epimerase

Protein Analysis

393

Amino Acids

44.37

Weight (kDa)

5.4

Isoelectric Point (pI)

31.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Epimerase	PF01370	32 - 212	5.3e-08	NAD dependent epimerase/dehydratase family
PRISE	PF22917	90 - 307	2.4e-26	PRISE-like Rossmann-fold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000626)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24220 AT4G24220
fragaria_vesca	FvH4_2g04580 FvH4_2g04580 FvH4_2g04580 FvH4_4g06071 FvH4_5g25130
malus_domestica	MD05G1094500.v1.1 MD05G1108300.v1.1 MD08G1088400.v1.1 MD15G1073200.v1.1 MD15G1073300.v1.1 MD15G1073400.v1.1 MD15G1073500.v1.1 MD15G1073600.v1.1
prunus_persica	Prupe.1G426100_v2.0.a1 Prupe.1G426100_v2.0.a1 Prupe.1G426200_v2.0.a1 Prupe.1G426300_v2.0.a1 Prupe.1G426400_v2.0.a1 Prupe.1G426400_v2.0.a1 Prupe.8G139400_v2.0.a1 Prupe.8G139400_v2.0.a1
pyrus_communis	pycom05g09350 pycom08g07190 pycom13g22780 pycom15g06840 pycom15g06850 pycom15g06870
rosa_chinensis	RchiOBHm_Chr6g0254161 RchiOBHm_Chr6g0254181 RchiOBHm_Chr6g0254191 RchiOBHm_Chr6g0309171 RchiOBHm_Chr6g0309181 RchiOBHm_Chr6g0309191 RchiOBHm_Chr6g0309201
rosa_laevigata	RLG00000010582 RLG00000010583 RLG00000014937 RLG00000014938
rosa_multiflora	Rmu_co8060540.1_g000001 Rmu_co8332663.1_g000001 Rmu_sc0000010.1_g000017 Rmu_sc0003711.1_g000008 Rmu_sc0016971.1_g000003 Rmu_sc0020102.1_g000006 Rmu_sc0020102.1_g000008
rosa_roxburghii	Rroxscaffold_7G00159570 Rroxscaffold_7G00159580 Rroxscaffold_7G00159590 Rroxscaffold_7G00211100
rosa_rugosa	Rorug05G0550400 Rorug06G0370100 Rorug06G0370300
rosa_samantha	Rh6AG066900 Rh6AG483200 Rh6AG483300 Rh6AG483400 Rh6BG059800 Rh6BG060000 Rh6BG492200 Rh6BG492300 Rh6BG492500 Rh6CG059700 Rh6CG059800 Rh6CG496900 Rh6CG497000 Rh6CG497200 Rh6CG497400 Rh6DG056700 Rh6DG056800 Rh6DG483800 Rh6DG484000
rosa_wichuraiana	Rw6G005860 Rw6G005870 Rw6G042070 Rw6G042080 Rw6G042090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	331
AbsI	CCTCGAGG	1 cut(s)	551
Acc16I	TGCGCA	2 cut(s)	377, 389
AccB7I	CCANNNNNTGG	1 cut(s)	229
AccBSI	CCGCTC	1 cut(s)	148
AccII	CGCG	5 cut(s)	202, 407, 520, 530, 680
AclI	AACGTT	1 cut(s)	381
AclWI	GGATC	3 cut(s)	519, 774, 1050
AcsI	RAATTY	2 cut(s)	713, 1126
AcvI	CACGTG	1 cut(s)	424
AcyI	GRCGYC	1 cut(s)	196
AdeI	CACNNNGTG	1 cut(s)	229
AfaI	GTAC	2 cut(s)	677, 905
AfiI	CCNNNNNNNGG	2 cut(s)	229, 609
AflIII	ACRYGT	2 cut(s)	423, 558
AgeI	ACCGGT	1 cut(s)	330
AgsI	TTSAA	5 cut(s)	29, 402, 526, 712, 853
AhlI	ACTAGT	1 cut(s)	1073
AjiI	CACGTC	1 cut(s)	559
AjnI	CCWGG	1 cut(s)	160
AjuI	GAANNNNNNNTTGG	2 cut(s)	920, 952
AloI	GAACNNNNNNTCC	2 cut(s)	1034, 1066
AluBI	AGCT	7 cut(s)	6, 32, 280, 931, 936, 997, 1160
AluI	AGCT	7 cut(s)	6, 32, 280, 931, 936, 997, 1160
Alw26I	GTCTC	1 cut(s)	461
AlwI	GGATC	3 cut(s)	519, 774, 1050
Ama87I	CYCGRG	2 cut(s)	551, 614
AoxI	GGCC	2 cut(s)	357, 611
ApeKI	GCWGC	2 cut(s)	32, 781
ApoI	RAATTY	2 cut(s)	713, 1126
AsiGI	ACCGGT	1 cut(s)	330
AspLEI	GCGC	5 cut(s)	204, 378, 390, 409, 532
AspS9I	GGNCC	6 cut(s)	167, 333, 458, 600, 612, 802
AsuC2I	CCSGG	4 cut(s)	260, 615, 616, 720
AsuHPI	GGTGA	3 cut(s)	109, 935, 967
AvaI	CYCGRG	2 cut(s)	551, 614
AvaII	GGWCC	5 cut(s)	167, 333, 458, 600, 802
BbrPI	CACGTG	1 cut(s)	424
BbvI	GCAGC	2 cut(s)	19, 793
BcgI	CGANNNNNNTGC	4 cut(s)	112, 146, 344, 378
BciT130I	CCWGG	1 cut(s)	162
BciVI	GTATCC	1 cut(s)	86
BclI	TGATCA	1 cut(s)	484
BcnI	CCSGG	4 cut(s)	260, 615, 616, 720
BcoDI	GTCTC	1 cut(s)	461
BcuI	ACTAGT	1 cut(s)	1073
BfaI	CTAG	2 cut(s)	192, 1074
BfuI	GTATCC	1 cut(s)	86
BisI	GCNGC	6 cut(s)	33, 366, 518, 681, 782, 795
BlpI	GCTNAGC	2 cut(s)	882, 932
BlsI	GCNGC	6 cut(s)	34, 367, 519, 682, 783, 796
Bme1390I	CCNGG	5 cut(s)	162, 260, 615, 616, 720
Bme18I	GGWCC	5 cut(s)	167, 333, 458, 600, 802
BmeT110I	CYCGRG	2 cut(s)	551, 614
BmgBI	CACGTC	1 cut(s)	559
BmgT120I	GGNCC	6 cut(s)	167, 333, 458, 600, 612, 802
BmiI	GGNNCC	3 cut(s)	442, 804, 1002
BmrFI	CCNGG	5 cut(s)	162, 260, 615, 616, 720
BmsI	GCATC	4 cut(s)	399, 693, 742, 751
BpmI	CTGGAG	1 cut(s)	1019
Bpu1102I	GCTNAGC	2 cut(s)	882, 932
BpuMI	CCSGG	4 cut(s)	260, 615, 616, 720
BsaAI	YACGTR	1 cut(s)	424
BsaBI	GATNNNNATC	1 cut(s)	765
BsaHI	GRCGYC	1 cut(s)	196
BsaJI	CCNNGG	3 cut(s)	170, 614, 615
BsaWI	WCCGGW	1 cut(s)	330
BsaXI	ACNNNNNCTCC	2 cut(s)	893, 923
Bsc4I	CCNNNNNNNGG	2 cut(s)	229, 609
Bse118I	RCCGGY	1 cut(s)	330
Bse1I	ACTGG	2 cut(s)	215, 570
Bse8I	GATNNNNATC	1 cut(s)	765
BseBI	CCWGG	1 cut(s)	162
BseDI	CCNNGG	3 cut(s)	170, 614, 615
BseGI	GGATG	2 cut(s)	708, 850
BseJI	GATNNNNATC	1 cut(s)	765
BseLI	CCNNNNNNNGG	2 cut(s)	229, 609
BseMII	CTCAG	5 cut(s)	267, 330, 659, 873, 923
BseNI	ACTGG	2 cut(s)	215, 570
BseRI	GAGGAG	2 cut(s)	914, 932
BseXI	GCAGC	2 cut(s)	19, 793
Bsh1236I	CGCG	5 cut(s)	202, 407, 520, 530, 680
BshFI	GGCC	2 cut(s)	359, 613
BshTI	ACCGGT	1 cut(s)	330
BsiHKCI	CYCGRG	2 cut(s)	551, 614
BsiSI	CCGG	4 cut(s)	260, 331, 615, 720
BslI	CCNNNNNNNGG	2 cut(s)	229, 609
BsmAI	GTCTC	1 cut(s)	461
BsmBI	CGTCTC	1 cut(s)	461
BsnI	GGCC	2 cut(s)	359, 613
BsoBI	CYCGRG	2 cut(s)	551, 614
Bsp143I	GATC	7 cut(s)	55, 99, 484, 511, 766, 1020, 1042
Bsp1720I	GCTNAGC	2 cut(s)	882, 932
Bsp19I	CCATGG	1 cut(s)	170
BspANI	GGCC	2 cut(s)	359, 613
BspCNI	CTCAG	5 cut(s)	266, 331, 660, 874, 924
BspFNI	CGCG	5 cut(s)	202, 407, 520, 530, 680
BspHI	TCATGA	1 cut(s)	490
BspLI	GGNNCC	3 cut(s)	442, 804, 1002
BspPI	GGATC	3 cut(s)	519, 774, 1050
BsrBI	CCGCTC	1 cut(s)	148
BsrFI	RCCGGY	1 cut(s)	330
BsrI	ACTGG	2 cut(s)	215, 570
BssAI	RCCGGY	1 cut(s)	330
BssECI	CCNNGG	3 cut(s)	170, 614, 615
BssMI	GATC	7 cut(s)	55, 99, 484, 511, 766, 1020, 1042
BssNI	GRCGYC	1 cut(s)	196
BssT1I	CCWWGG	1 cut(s)	170
Bst2UI	CCWGG	1 cut(s)	162
Bst4CI	ACNGT	2 cut(s)	243, 1029
Bst6I	CTCTTC	1 cut(s)	564
BstACI	GRCGYC	1 cut(s)	196
BstBAI	YACGTR	1 cut(s)	424
BstC8I	GCNNGC	3 cut(s)	206, 431, 692
BstDEI	CTNAG	5 cut(s)	253, 339, 668, 882, 932
BstDSI	CCRYGG	1 cut(s)	170
BstF5I	GGATG	2 cut(s)	708, 850
BstFNI	CGCG	5 cut(s)	202, 407, 520, 530, 680
BstHHI	GCGC	5 cut(s)	204, 378, 390, 409, 532
BstKTI	GATC	7 cut(s)	58, 102, 487, 514, 769, 1023, 1045
BstMAI	GTCTC	1 cut(s)	461
BstMBI	GATC	7 cut(s)	55, 99, 484, 511, 766, 1020, 1042
BstMWI	GCNNNNNNNGC	4 cut(s)	29, 224, 365, 761
BstNI	CCWGG	1 cut(s)	162
BstSCI	CCNGG	5 cut(s)	160, 258, 613, 614, 718
BstUI	CGCG	5 cut(s)	202, 407, 520, 530, 680
BstV1I	GCAGC	2 cut(s)	19, 793
BstX2I	RGATCY	3 cut(s)	511, 766, 1042
BstYI	RGATCY	3 cut(s)	511, 766, 1042
BsuI	GTATCC	1 cut(s)	86
BsuRI	GGCC	2 cut(s)	359, 613
BtgI	CCRYGG	1 cut(s)	170
BtrI	CACGTC	1 cut(s)	559
BtsCI	GGATG	2 cut(s)	708, 850
BtsI	GCAGTG	1 cut(s)	234
BtsIMutI	CAGTG	5 cut(s)	208, 234, 248, 336, 1034
Cac8I	GCNNGC	3 cut(s)	206, 431, 692
CciI	TCATGA	1 cut(s)	490
CfoI	GCGC	5 cut(s)	204, 378, 390, 409, 532
Cfr10I	RCCGGY	1 cut(s)	330
Cfr13I	GGNCC	6 cut(s)	167, 333, 458, 600, 612, 802
Cfr9I	CCCGGG	1 cut(s)	614
CseI	GACGC	1 cut(s)	185
Csp6I	GTAC	2 cut(s)	676, 904
CspAI	ACCGGT	1 cut(s)	330
CviAII	CATG	6 cut(s)	171, 370, 491, 655, 664, 1097
CviQI	GTAC	2 cut(s)	676, 904
DdeI	CTNAG	5 cut(s)	253, 339, 668, 882, 932
DpnI	GATC	7 cut(s)	57, 101, 486, 513, 768, 1022, 1044
DpnII	GATC	7 cut(s)	55, 99, 484, 511, 766, 1020, 1042
DraIII	CACNNNGTG	1 cut(s)	229
DrdI	GACNNNNNNGTC	1 cut(s)	331
DseDI	GACNNNNNNGTC	1 cut(s)	331
Eam1104I	CTCTTC	1 cut(s)	564
EarI	CTCTTC	1 cut(s)	564
EciI	GGCGGA	1 cut(s)	956
Eco130I	CCWWGG	1 cut(s)	170
Eco32I	GATATC	1 cut(s)	592
Eco47I	GGWCC	5 cut(s)	167, 333, 458, 600, 802
Eco72I	CACGTG	1 cut(s)	424
Eco88I	CYCGRG	2 cut(s)	551, 614
EcoRI	GAATTC	1 cut(s)	1126
EcoRII	CCWGG	1 cut(s)	160
EcoRV	GATATC	1 cut(s)	592
EcoT14I	CCWWGG	1 cut(s)	170
ErhI	CCWWGG	1 cut(s)	170
Esp3I	CGTCTC	1 cut(s)	461
FaeI	CATG	6 cut(s)	174, 373, 494, 658, 667, 1100
FalI	AAGNNNNNCTT	2 cut(s)	920, 952
FatI	CATG	6 cut(s)	170, 369, 490, 654, 663, 1096
FauI	CCCGC	3 cut(s)	153, 213, 504
FbaI	TGATCA	1 cut(s)	484
Fnu4HI	GCNGC	6 cut(s)	33, 366, 518, 681, 782, 795
FokI	GGATG	2 cut(s)	715, 857
Fsp4HI	GCNGC	6 cut(s)	33, 366, 518, 681, 782, 795
FspBI	CTAG	2 cut(s)	192, 1074
FspI	TGCGCA	2 cut(s)	377, 389
GlaI	GCGC	5 cut(s)	203, 377, 389, 408, 531
GluI	GCNGC	6 cut(s)	33, 366, 518, 681, 782, 795
GsuI	CTGGAG	1 cut(s)	1019
HaeIII	GGCC	2 cut(s)	359, 613
HapII	CCGG	4 cut(s)	260, 331, 615, 720
HgaI	GACGC	1 cut(s)	185
HhaI	GCGC	5 cut(s)	204, 378, 390, 409, 532
Hin1I	GRCGYC	1 cut(s)	196
Hin1II	CATG	6 cut(s)	174, 373, 494, 658, 667, 1100
Hin6I	GCGC	5 cut(s)	202, 376, 388, 407, 530
HinP1I	GCGC	5 cut(s)	202, 376, 388, 407, 530
HincII	GTYRAC	1 cut(s)	835
HindII	GTYRAC	1 cut(s)	835
HindIII	AAGCTT	1 cut(s)	1158
HinfI	GANTC	1 cut(s)	412
HpaII	CCGG	4 cut(s)	260, 331, 615, 720
HphI	GGTGA	3 cut(s)	109, 935, 967
Hpy166II	GTNNAC	5 cut(s)	232, 336, 676, 802, 835
Hpy188I	TCNGA	8 cut(s)	154, 256, 458, 508, 751, 760, 771, 1025
Hpy188III	TCNNGA	2 cut(s)	466, 491
Hpy8I	GTNNAC	5 cut(s)	232, 336, 676, 802, 835
HpyAV	CCTTC	7 cut(s)	169, 577, 692, 865, 946, 979, 1055
HpyCH4III	ACNGT	2 cut(s)	243, 1029
HpyCH4IV	ACGT	6 cut(s)	297, 315, 381, 423, 470, 558
HpyCH4V	TGCA	2 cut(s)	433, 690
HpyF10VI	GCNNNNNNNGC	4 cut(s)	29, 224, 365, 761
HpyF3I	CTNAG	5 cut(s)	253, 339, 668, 882, 932
HpySE526I	ACGT	6 cut(s)	297, 315, 381, 423, 470, 558
Hsp92I	GRCGYC	1 cut(s)	196
Hsp92II	CATG	6 cut(s)	174, 373, 494, 658, 667, 1100
HspAI	GCGC	5 cut(s)	202, 376, 388, 407, 530
Ksp22I	TGATCA	1 cut(s)	484
Kzo9I	GATC	7 cut(s)	55, 99, 484, 511, 766, 1020, 1042
LmnI	GCTCC	3 cut(s)	778, 973, 1000
Lsp1109I	GCAGC	2 cut(s)	19, 793
LweI	GCATC	4 cut(s)	399, 693, 742, 751
MaeI	CTAG	2 cut(s)	192, 1074
MaeII	ACGT	6 cut(s)	297, 315, 381, 423, 470, 558
MaeIII	GTNAC	6 cut(s)	59, 293, 298, 316, 419, 561
MalI	GATC	7 cut(s)	57, 101, 486, 513, 768, 1022, 1044
MbiI	CCGCTC	1 cut(s)	148
MboI	GATC	7 cut(s)	55, 99, 484, 511, 766, 1020, 1042
MboII	GAAGA	5 cut(s)	52, 65, 301, 581, 592
MflI	RGATCY	3 cut(s)	511, 766, 1042
MluCI	AATT	6 cut(s)	287, 349, 480, 535, 713, 1126
MmeI	TCCRAC	2 cut(s)	177, 1121
MseI	TTAA	3 cut(s)	290, 828, 1164
MspA1I	CMGCKG	1 cut(s)	997
MspI	CCGG	4 cut(s)	260, 331, 615, 720
MspR9I	CCNGG	5 cut(s)	162, 260, 615, 616, 720
MvaI	CCWGG	1 cut(s)	162
MvnI	CGCG	5 cut(s)	202, 407, 520, 530, 680
MwoI	GCNNNNNNNGC	4 cut(s)	29, 224, 365, 761
NciI	CCSGG	4 cut(s)	260, 615, 616, 720
NcoI	CCATGG	1 cut(s)	170
NdeII	GATC	7 cut(s)	55, 99, 484, 511, 766, 1020, 1042
NlaIII	CATG	6 cut(s)	174, 373, 494, 658, 667, 1100
NlaIV	GGNNCC	3 cut(s)	442, 804, 1002
NmuCI	GTSAC	2 cut(s)	293, 419
NsbI	TGCGCA	2 cut(s)	377, 389
PaeR7I	CTCGAG	1 cut(s)	551
PagI	TCATGA	1 cut(s)	490
PcsI	WCGNNNNNNNCGW	2 cut(s)	351, 462
PfeI	GAWTC	1 cut(s)	412
PflMI	CCANNNNNTGG	1 cut(s)	229
PfoI	TCCNGGA	1 cut(s)	718
PinAI	ACCGGT	1 cut(s)	330
PkrI	GCNGC	6 cut(s)	34, 367, 519, 682, 783, 796
PmaCI	CACGTG	1 cut(s)	424
PmlI	CACGTG	1 cut(s)	424
Ppu21I	YACGTR	1 cut(s)	424
Psp1406I	AACGTT	1 cut(s)	381
Psp6I	CCWGG	1 cut(s)	160
PspCI	CACGTG	1 cut(s)	424
PspGI	CCWGG	1 cut(s)	160
PspN4I	GGNNCC	3 cut(s)	442, 804, 1002
PspPI	GGNCC	6 cut(s)	167, 333, 458, 600, 612, 802
PspXI	VCTCGAGB	1 cut(s)	551
PsuI	RGATCY	3 cut(s)	511, 766, 1042
PvuII	CAGCTG	1 cut(s)	997
RsaI	GTAC	2 cut(s)	677, 905
RsaNI	GTAC	2 cut(s)	676, 904
SaqAI	TTAA	3 cut(s)	290, 828, 1164
SatI	GCNGC	6 cut(s)	33, 366, 518, 681, 782, 795
Sau3AI	GATC	7 cut(s)	55, 99, 484, 511, 766, 1020, 1042
Sau96I	GGNCC	6 cut(s)	167, 333, 458, 600, 612, 802
ScrFI	CCNGG	5 cut(s)	162, 260, 615, 616, 720
SfaNI	GCATC	4 cut(s)	399, 693, 742, 751
Sfr274I	CTCGAG	1 cut(s)	551
SinI	GGWCC	5 cut(s)	167, 333, 458, 600, 802
SlaI	CTCGAG	1 cut(s)	551
SmaI	CCCGGG	1 cut(s)	616
SmlI	CTYRAG	1 cut(s)	551
SmoI	CTYRAG	1 cut(s)	551
SpeI	ACTAGT	1 cut(s)	1073
Sse9I	AATT	6 cut(s)	287, 349, 480, 535, 713, 1126
SspMI	CTAG	2 cut(s)	192, 1074
StyD4I	CCNGG	5 cut(s)	160, 258, 613, 614, 718
StyI	CCWWGG	1 cut(s)	170
TaaI	ACNGT	2 cut(s)	243, 1029
TaiI	ACGT	6 cut(s)	300, 318, 384, 426, 473, 561
TaqI	TCGA	2 cut(s)	465, 552
TasI	AATT	6 cut(s)	287, 349, 480, 535, 713, 1126
TauI	GCSGC	4 cut(s)	368, 520, 683, 797
TfiI	GAWTC	1 cut(s)	412
Tru1I	TTAA	3 cut(s)	290, 828, 1164
Tru9I	TTAA	3 cut(s)	290, 828, 1164
TscAI	CASTG	5 cut(s)	215, 234, 248, 343, 1034
TseFI	GTSAC	2 cut(s)	293, 419
TseI	GCWGC	2 cut(s)	32, 781
Tsp45I	GTSAC	2 cut(s)	293, 419
TspDTI	ATGAA	3 cut(s)	965, 1022, 1094
TspGWI	ACGGA	2 cut(s)	407, 592
TspMI	CCCGGG	1 cut(s)	614
TspRI	CASTG	5 cut(s)	215, 234, 248, 343, 1034
Van91I	CCANNNNNTGG	1 cut(s)	229
VpaK11BI	GGWCC	5 cut(s)	167, 333, 458, 600, 802
XapI	RAATTY	2 cut(s)	713, 1126
XhoI	CTCGAG	1 cut(s)	551
XmaI	CCCGGG	1 cut(s)	614
XspI	CTAG	2 cut(s)	192, 1074

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.