FvH4_2g04580

3-oxo-Delta(4,5)-steroid 5-beta-reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000626
Physical Location & Seq
Reverse (-)
3516995 .. 3518722
1728 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g04580.t3

Sequence Viewer

Length: 807 bp
ATGTTGAGTAATGTCTTGAATGCTGTGATCCCAAACGCGCCGAATTTGCAGCACATATGTATGCAGACAGGTCGGAAGCAATACGTAGGTCCGTTTGAGATGTTTGGGAAAATCGAACCGCATGAGCCACCATATGTCGAAGACACGTCGAGGCTTGATTGCCCGAATTTCTACTATGTGATGGAGGATATTCTGTTTGAGGAGGTGAAGAAGAAGGAGGGGCTGACATGGTCTGTGCATAGGCCGGGAATCATATTCGGGTTTTCGCCTTATAGCTTGATAAATACGATTGGGAGTTTGTGTGTTTACGCTGCGATTTGTAAGCATGAAGGGCAGCCGTTGAGGTTTCCGGGGAGTAAAGGAACTTGGGAGGGGTTCTGGGATGCTTCTGATGCAGATATGATTGCTGAACATGAGATATGGGCAGCTGTGGAGCCTTATGCCAAGAATGAAGCTTTCAATTGCAGCAATGGGGATGTGTTCAGGTGGAAGCATTTGTGGGGGATTTTGGCTGATCAGTTCGAGGTCGAGTCTGGTGGAGTTGATGAGGAGTTGAGGGTAGAGGAGATGATGAAAGATAAAGGGCCAGTGTGGGATGAGATTGTGAGAAAGCATGATTTGGTGCCTTCCAAATTGGAAGAAGTTGGGAACTGGTGGCTGGTGGATATTATATTTCGGATTGGGGTTGATTCGAGTATGGACAGTATGAATAAGAGCAGAGAGCATGGTTTTGTTGGCTTTAGGAACTCGAAAACTTCCTTCCTTTCTTGGATTGACAAGATGAAGTCTTACAGAATTGTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

269

Amino Acids

30.81

Weight (kDa)

5.16

Isoelectric Point (pI)

37.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRISE PF22917 29 - 389 1e-22 PRISE-like Rossmann-fold domain
Epimerase PF01370 29 - 181 6.7e-06 NAD dependent epimerase/dehydratase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000626)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24220 AT4G24220
fragaria_vesca FvH4_2g04580 FvH4_2g04580 FvH4_2g04580 FvH4_4g06071 FvH4_5g25130
malus_domestica MD05G1094500.v1.1 MD05G1108300.v1.1 MD08G1088400.v1.1 MD15G1073200.v1.1 MD15G1073300.v1.1 MD15G1073400.v1.1 MD15G1073500.v1.1 MD15G1073600.v1.1
prunus_persica Prupe.1G426100_v2.0.a1 Prupe.1G426100_v2.0.a1 Prupe.1G426200_v2.0.a1 Prupe.1G426300_v2.0.a1 Prupe.1G426400_v2.0.a1 Prupe.1G426400_v2.0.a1 Prupe.8G139400_v2.0.a1 Prupe.8G139400_v2.0.a1
pyrus_communis pycom05g09350 pycom08g07190 pycom13g22780 pycom15g06840 pycom15g06850 pycom15g06870
rosa_chinensis RchiOBHm_Chr6g0254161 RchiOBHm_Chr6g0254181 RchiOBHm_Chr6g0254191 RchiOBHm_Chr6g0309171 RchiOBHm_Chr6g0309181 RchiOBHm_Chr6g0309191 RchiOBHm_Chr6g0309201
rosa_laevigata RLG00000010582 RLG00000010583 RLG00000014937 RLG00000014938
rosa_multiflora Rmu_co8060540.1_g000001 Rmu_co8332663.1_g000001 Rmu_sc0000010.1_g000017 Rmu_sc0003711.1_g000008 Rmu_sc0016971.1_g000003 Rmu_sc0020102.1_g000006 Rmu_sc0020102.1_g000008
rosa_roxburghii Rroxscaffold_7G00159570 Rroxscaffold_7G00159580 Rroxscaffold_7G00159590 Rroxscaffold_7G00211100
rosa_rugosa Rorug05G0550400 Rorug06G0370100 Rorug06G0370300
rosa_samantha Rh6AG066900 Rh6AG483200 Rh6AG483300 Rh6AG483400 Rh6BG059800 Rh6BG060000 Rh6BG492200 Rh6BG492300 Rh6BG492500 Rh6CG059700 Rh6CG059800 Rh6CG496900 Rh6CG497000 Rh6CG497200 Rh6CG497400 Rh6DG056700 Rh6DG056800 Rh6DG483800 Rh6DG484000
rosa_wichuraiana Rw6G005860 Rw6G005870 Rw6G042070 Rw6G042080 Rw6G042090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 622
AccII CGCG 1 cut(s) 38
AciI CCGC 1 cut(s) 119
AclWI GGATC 1 cut(s) 22
AcsI RAATTY 2 cut(s) 43, 166
AflIII ACRYGT 1 cut(s) 144
AgsI TTSAA 2 cut(s) 19, 460
AjiI CACGTC 1 cut(s) 147
AluBI AGCT 3 cut(s) 276, 428, 455
AluI AGCT 3 cut(s) 276, 428, 455
AlwI GGATC 1 cut(s) 22
AoxI GGCC 2 cut(s) 242, 584
ApeKI GCWGC 5 cut(s) 49, 311, 334, 425, 465
ApoI RAATTY 2 cut(s) 43, 166
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 2 cut(s) 89, 584
AsuC2I CCSGG 2 cut(s) 246, 351
AsuHPI GGTGA 1 cut(s) 217
AvaII GGWCC 1 cut(s) 89
BanI GGYRCC 1 cut(s) 622
BbsI GAAGAC 1 cut(s) 147
BbvI GCAGC 5 cut(s) 61, 298, 346, 437, 477
BccI CCATC 1 cut(s) 175
BceAI ACGGC 1 cut(s) 322
BcgI CGANNNNNNTGC 2 cut(s) 53, 87
BclI TGATCA 1 cut(s) 514
BcnI CCSGG 2 cut(s) 246, 351
BisI GCNGC 5 cut(s) 50, 312, 335, 426, 466
BlsI GCNGC 5 cut(s) 51, 313, 336, 427, 467
Bme1390I CCNGG 2 cut(s) 246, 351
Bme18I GGWCC 1 cut(s) 89
BmgBI CACGTC 1 cut(s) 147
BmgT120I GGNCC 2 cut(s) 89, 584
BmiI GGNNCC 2 cut(s) 435, 624
BmrFI CCNGG 2 cut(s) 246, 351
BmsI GCATC 2 cut(s) 373, 382
BpiI GAAGAC 1 cut(s) 147
BpuMI CCSGG 2 cut(s) 246, 351
BsaAI YACGTR 1 cut(s) 85
BsaJI CCNNGG 1 cut(s) 350
BsaXI ACNNNNNCTCC 2 cut(s) 286, 316
Bse1I ACTGG 2 cut(s) 587, 656
Bse3DI GCAATG 1 cut(s) 475
BseDI CCNNGG 1 cut(s) 350
BseGI GGATG 3 cut(s) 388, 481, 601
BseMI GCAATG 1 cut(s) 475
BseNI ACTGG 2 cut(s) 587, 656
BseRI GAGGAG 3 cut(s) 215, 563, 578
BseXI GCAGC 5 cut(s) 61, 298, 346, 437, 477
Bsh1236I CGCG 1 cut(s) 38
BshFI GGCC 2 cut(s) 244, 586
BshNI GGYRCC 1 cut(s) 622
BsiSI CCGG 2 cut(s) 245, 350
BsmI GAATGC 1 cut(s) 25
BsnI GGCC 2 cut(s) 244, 586
Bsp143I GATC 2 cut(s) 27, 514
BspACI CCGC 1 cut(s) 119
BspANI GGCC 2 cut(s) 244, 586
BspFNI CGCG 1 cut(s) 38
BspLI GGNNCC 2 cut(s) 435, 624
BspPI GGATC 1 cut(s) 22
BspT107I GGYRCC 1 cut(s) 622
BsrDI GCAATG 1 cut(s) 475
BsrI ACTGG 2 cut(s) 587, 656
BssECI CCNNGG 1 cut(s) 350
BssMI GATC 2 cut(s) 27, 514
Bst4CI ACNGT 1 cut(s) 704
BstBAI YACGTR 1 cut(s) 85
BstF5I GGATG 3 cut(s) 388, 481, 601
BstFNI CGCG 1 cut(s) 38
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 2 cut(s) 30, 517
BstMBI GATC 2 cut(s) 27, 514
BstMWI GCNNNNNNNGC 3 cut(s) 46, 331, 392
BstSCI CCNGG 2 cut(s) 244, 349
BstSNI TACGTA 1 cut(s) 85
BstUI CGCG 1 cut(s) 38
BstV1I GCAGC 5 cut(s) 61, 298, 346, 437, 477
BstV2I GAAGAC 1 cut(s) 147
BsuRI GGCC 2 cut(s) 244, 586
BtrI CACGTC 1 cut(s) 147
BtsCI GGATG 3 cut(s) 388, 481, 601
BtsIMutI CAGTG 1 cut(s) 594
CfoI GCGC 1 cut(s) 40
Cfr13I GGNCC 2 cut(s) 89, 584
CviAII CATG 6 cut(s) 122, 228, 326, 413, 614, 725
DpnI GATC 2 cut(s) 29, 516
DpnII GATC 2 cut(s) 27, 514
Eco105I TACGTA 1 cut(s) 85
Eco47I GGWCC 1 cut(s) 89
FaeI CATG 6 cut(s) 125, 231, 329, 416, 617, 728
FatI CATG 6 cut(s) 121, 227, 325, 412, 613, 724
FauNDI CATATG 2 cut(s) 56, 133
FbaI TGATCA 1 cut(s) 514
Fnu4HI GCNGC 5 cut(s) 50, 312, 335, 426, 466
FokI GGATG 3 cut(s) 395, 488, 608
Fsp4HI GCNGC 5 cut(s) 50, 312, 335, 426, 466
GlaI GCGC 1 cut(s) 39
GluI GCNGC 5 cut(s) 50, 312, 335, 426, 466
HaeIII GGCC 2 cut(s) 244, 586
HapII CCGG 2 cut(s) 245, 350
HhaI GCGC 1 cut(s) 40
Hin1II CATG 6 cut(s) 125, 231, 329, 416, 617, 728
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HindIII AAGCTT 1 cut(s) 453
HinfI GANTC 3 cut(s) 249, 530, 689
HpaII CCGG 2 cut(s) 245, 350
HphI GGTGA 1 cut(s) 217
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 3 cut(s) 75, 391, 678
Hpy188III TCNNGA 1 cut(s) 16
Hpy8I GTNNAC 1 cut(s) 307
Hpy99I CGWCG 1 cut(s) 151
HpyAV CCTTC 4 cut(s) 208, 323, 636, 769
HpyCH4III ACNGT 1 cut(s) 704
HpyCH4IV ACGT 2 cut(s) 84, 146
HpyCH4V TGCA 5 cut(s) 49, 64, 238, 395, 465
HpyF10VI GCNNNNNNNGC 3 cut(s) 46, 331, 392
HpySE526I ACGT 2 cut(s) 84, 146
Hsp92II CATG 6 cut(s) 125, 231, 329, 416, 617, 728
HspAI GCGC 1 cut(s) 38
Ksp22I TGATCA 1 cut(s) 514
Kzo9I GATC 2 cut(s) 27, 514
LmnI GCTCC 1 cut(s) 433
LpnPI CCDG 9 cut(s) 54, 258, 363, 364, 469, 519, 600, 637, 644
Lsp1109I GCAGC 5 cut(s) 61, 298, 346, 437, 477
LweI GCATC 2 cut(s) 373, 382
MaeII ACGT 2 cut(s) 84, 146
MalI GATC 2 cut(s) 29, 516
MboI GATC 2 cut(s) 27, 514
MboII GAAGA 4 cut(s) 152, 220, 223, 650
MfeI CAATTG 1 cut(s) 460
MluCI AATT 5 cut(s) 43, 166, 460, 632, 795
MlyI GAGTC 1 cut(s) 539
MmeI TCCRAC 1 cut(s) 53
MslI CAYNNNNRTG 1 cut(s) 59
MspA1I CMGCKG 1 cut(s) 428
MspI CCGG 2 cut(s) 245, 350
MspR9I CCNGG 2 cut(s) 246, 351
MunI CAATTG 1 cut(s) 460
Mva1269I GAATGC 1 cut(s) 25
MvnI CGCG 1 cut(s) 38
MwoI GCNNNNNNNGC 3 cut(s) 46, 331, 392
NciI CCSGG 2 cut(s) 246, 351
NdeI CATATG 2 cut(s) 56, 133
NdeII GATC 2 cut(s) 27, 514
NlaIII CATG 6 cut(s) 125, 231, 329, 416, 617, 728
NlaIV GGNNCC 2 cut(s) 435, 624
PctI GAATGC 1 cut(s) 25
PfeI GAWTC 2 cut(s) 249, 689
PflFI GACNNNGTC 1 cut(s) 229
PkrI GCNGC 5 cut(s) 51, 313, 336, 427, 467
PleI GAGTC 1 cut(s) 538
PpsI GAGTC 1 cut(s) 538
Ppu21I YACGTR 1 cut(s) 85
PspN4I GGNNCC 2 cut(s) 435, 624
PspPI GGNCC 2 cut(s) 89, 584
PsyI GACNNNGTC 1 cut(s) 229
PvuII CAGCTG 1 cut(s) 428
RseI CAYNNNNRTG 1 cut(s) 59
SatI GCNGC 5 cut(s) 50, 312, 335, 426, 466
Sau3AI GATC 2 cut(s) 27, 514
Sau96I GGNCC 2 cut(s) 89, 584
SchI GAGTC 1 cut(s) 539
ScrFI CCNGG 2 cut(s) 246, 351
SfaNI GCATC 2 cut(s) 373, 382
SinI GGWCC 1 cut(s) 89
SmiMI CAYNNNNRTG 1 cut(s) 59
SnaBI TACGTA 1 cut(s) 85
Sse9I AATT 5 cut(s) 43, 166, 460, 632, 795
SsiI CCGC 1 cut(s) 119
StyD4I CCNGG 2 cut(s) 244, 349
TaaI ACNGT 1 cut(s) 704
TaiI ACGT 2 cut(s) 87, 149
TaqI TCGA 7 cut(s) 114, 138, 149, 522, 528, 692, 749
TasI AATT 5 cut(s) 43, 166, 460, 632, 795
TfiI GAWTC 2 cut(s) 249, 689
TscAI CASTG 1 cut(s) 594
TseI GCWGC 5 cut(s) 49, 311, 334, 425, 465
TspDTI ATGAA 5 cut(s) 342, 465, 587, 722, 797
TspGWI ACGGA 1 cut(s) 81
TspRI CASTG 1 cut(s) 594
Tth111I GACNNNGTC 1 cut(s) 229
VpaK11BI GGWCC 1 cut(s) 89
XapI RAATTY 2 cut(s) 43, 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.