3-oxo-Delta(4,5)-steroid 5-beta-reductase-like
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 197 |
| Acc16I | TGCGCA | 1 cut(s) | 347 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 58 |
| AccBSI | CCGCTC | 1 cut(s) | 1026 |
| AccI | GTMKAC | 1 cut(s) | 150 |
| AccII | CGCG | 5 cut(s) | 43, 161, 163, 377, 653 |
| AclI | AACGTT | 1 cut(s) | 351 |
| AclWI | GGATC | 5 cut(s) | 103, 221, 297, 489, 744 |
| AcsI | RAATTY | 5 cut(s) | 20, 358, 505, 1087, 1096 |
| AcuI | CTGAAG | 1 cut(s) | 701 |
| AcvI | CACGTG | 1 cut(s) | 394 |
| AcyI | GRCGYC | 2 cut(s) | 156, 725 |
| AdeI | CACNNNGTG | 1 cut(s) | 639 |
| AfaI | GTAC | 3 cut(s) | 206, 647, 875 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 58, 145, 409 |
| AflIII | ACRYGT | 1 cut(s) | 393 |
| AgsI | TTSAA | 2 cut(s) | 455, 823 |
| AjnI | CCWGG | 2 cut(s) | 130, 1091 |
| AluBI | AGCT | 6 cut(s) | 250, 385, 906, 916, 967, 982 |
| AluI | AGCT | 6 cut(s) | 250, 385, 906, 916, 967, 982 |
| Alw21I | GWGCWC | 1 cut(s) | 579 |
| Alw26I | GTCTC | 1 cut(s) | 68 |
| Alw44I | GTGCAC | 1 cut(s) | 575 |
| AlwI | GGATC | 5 cut(s) | 103, 221, 297, 489, 744 |
| AoxI | GGCC | 5 cut(s) | 136, 411, 428, 674, 892 |
| ApaLI | GTGCAC | 1 cut(s) | 575 |
| ApeKI | GCWGC | 5 cut(s) | 343, 751, 967, 970, 1044 |
| ApoI | RAATTY | 5 cut(s) | 20, 358, 505, 1087, 1096 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 408, 440, 518, 550 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 833, 1130 |
| AspLEI | GCGC | 4 cut(s) | 163, 165, 348, 379 |
| AspS9I | GGNCC | 5 cut(s) | 137, 294, 411, 428, 772 |
| AsuC2I | CCSGG | 2 cut(s) | 409, 690 |
| AsuHPI | GGTGA | 1 cut(s) | 70 |
| AvaII | GGWCC | 2 cut(s) | 294, 772 |
| BaeGI | GKGCMC | 1 cut(s) | 579 |
| BauI | CACGAG | 2 cut(s) | 65, 665 |
| BbrPI | CACGTG | 1 cut(s) | 394 |
| Bbv12I | GWGCWC | 1 cut(s) | 579 |
| BbvI | GCAGC | 5 cut(s) | 330, 763, 954, 982, 1056 |
| BceAI | ACGGC | 5 cut(s) | 150, 169, 320, 415, 1129 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 82, 116 |
| BciT130I | CCWGG | 2 cut(s) | 132, 1093 |
| BcnI | CCSGG | 2 cut(s) | 409, 690 |
| BcoDI | GTCTC | 1 cut(s) | 68 |
| BfmI | CTRYAG | 1 cut(s) | 968 |
| BlpI | GCTNAGC | 1 cut(s) | 902 |
| Bme1390I | CCNGG | 4 cut(s) | 132, 409, 690, 1093 |
| Bme18I | GGWCC | 2 cut(s) | 294, 772 |
| BmgT120I | GGNCC | 5 cut(s) | 137, 294, 411, 428, 772 |
| BmiI | GGNNCC | 3 cut(s) | 36, 412, 774 |
| BmrFI | CCNGG | 4 cut(s) | 132, 409, 690, 1093 |
| BmsI | GCATC | 4 cut(s) | 226, 487, 577, 721 |
| BpmI | CTGGAG | 2 cut(s) | 371, 386 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 902 |
| BpuEI | CTTGAG | 1 cut(s) | 542 |
| BpuMI | CCSGG | 2 cut(s) | 409, 690 |
| BsaAI | YACGTR | 1 cut(s) | 394 |
| BsaBI | GATNNNNATC | 2 cut(s) | 623, 735 |
| BsaHI | GRCGYC | 2 cut(s) | 156, 725 |
| BsaI | GGTCTC | 1 cut(s) | 68 |
| BsaJI | CCNNGG | 3 cut(s) | 140, 309, 408 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 923, 953, 1055, 1085 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 58, 145, 409 |
| Bse118I | RCCGGY | 1 cut(s) | 80 |
| Bse1I | ACTGG | 1 cut(s) | 185 |
| Bse8I | GATNNNNATC | 2 cut(s) | 623, 735 |
| BseBI | CCWGG | 2 cut(s) | 132, 1093 |
| BseDI | CCNNGG | 3 cut(s) | 140, 309, 408 |
| BseGI | GGATG | 5 cut(s) | 207, 502, 736, 820, 1018 |
| BseJI | GATNNNNATC | 2 cut(s) | 623, 735 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 58, 145, 409 |
| BseMII | CTCAG | 1 cut(s) | 893 |
| BseNI | ACTGG | 1 cut(s) | 185 |
| BsePI | GCGCGC | 1 cut(s) | 161 |
| BseRI | GAGGAG | 2 cut(s) | 884, 959 |
| BseSI | GKGCMC | 1 cut(s) | 579 |
| BseXI | GCAGC | 5 cut(s) | 330, 763, 954, 982, 1056 |
| BsgI | GTGCAG | 1 cut(s) | 1063 |
| Bsh1236I | CGCG | 5 cut(s) | 43, 161, 163, 377, 653 |
| BshFI | GGCC | 5 cut(s) | 138, 413, 430, 676, 894 |
| BsiHKAI | GWGCWC | 1 cut(s) | 579 |
| BsiSI | CCGG | 4 cut(s) | 81, 408, 689, 895 |
| BslFI | GGGAC | 2 cut(s) | 33, 153 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 58, 145, 409 |
| BsmAI | GTCTC | 1 cut(s) | 68 |
| BsmFI | GGGAC | 2 cut(s) | 33, 153 |
| BsnI | GGCC | 5 cut(s) | 138, 413, 430, 676, 894 |
| Bso31I | GGTCTC | 1 cut(s) | 68 |
| Bsp1286I | GDGCHC | 1 cut(s) | 579 |
| Bsp143I | GATC | 6 cut(s) | 69, 108, 226, 302, 481, 736 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 902 |
| Bsp19I | CCATGG | 1 cut(s) | 140 |
| BspANI | GGCC | 5 cut(s) | 138, 413, 430, 676, 894 |
| BspCNI | CTCAG | 1 cut(s) | 894 |
| BspFNI | CGCG | 5 cut(s) | 43, 161, 163, 377, 653 |
| BspLI | GGNNCC | 3 cut(s) | 36, 412, 774 |
| BspMAI | CTGCAG | 1 cut(s) | 972 |
| BspPI | GGATC | 5 cut(s) | 103, 221, 297, 489, 744 |
| BspTNI | GGTCTC | 1 cut(s) | 68 |
| BsrBI | CCGCTC | 1 cut(s) | 1026 |
| BsrFI | RCCGGY | 1 cut(s) | 80 |
| BsrI | ACTGG | 1 cut(s) | 185 |
| BssAI | RCCGGY | 1 cut(s) | 80 |
| BssECI | CCNNGG | 3 cut(s) | 140, 309, 408 |
| BssHII | GCGCGC | 1 cut(s) | 161 |
| BssMI | GATC | 6 cut(s) | 69, 108, 226, 302, 481, 736 |
| BssNAI | GTATAC | 1 cut(s) | 151 |
| BssNI | GRCGYC | 2 cut(s) | 156, 725 |
| BssSI | CACGAG | 2 cut(s) | 65, 665 |
| BssT1I | CCWWGG | 1 cut(s) | 140 |
| Bst1107I | GTATAC | 1 cut(s) | 151 |
| Bst2BI | CACGAG | 2 cut(s) | 65, 665 |
| Bst2UI | CCWGG | 2 cut(s) | 132, 1093 |
| Bst4CI | ACNGT | 3 cut(s) | 471, 534, 713 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstACI | GRCGYC | 2 cut(s) | 156, 725 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 639 |
| BstBAI | YACGTR | 1 cut(s) | 394 |
| BstC8I | GCNNGC | 5 cut(s) | 163, 635, 662, 849, 984 |
| BstDEI | CTNAG | 1 cut(s) | 902 |
| BstDSI | CCRYGG | 1 cut(s) | 140 |
| BstF5I | GGATG | 5 cut(s) | 207, 502, 736, 820, 1018 |
| BstFNI | CGCG | 5 cut(s) | 43, 161, 163, 377, 653 |
| BstHHI | GCGC | 4 cut(s) | 163, 165, 348, 379 |
| BstKTI | GATC | 6 cut(s) | 72, 111, 229, 305, 484, 739 |
| BstMAI | GTCTC | 1 cut(s) | 68 |
| BstMBI | GATC | 6 cut(s) | 69, 108, 226, 302, 481, 736 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 90, 162, 171, 545, 639, 731, 853, 979 |
| BstNI | CCWGG | 2 cut(s) | 132, 1093 |
| BstSCI | CCNGG | 4 cut(s) | 130, 407, 688, 1091 |
| BstSFI | CTRYAG | 1 cut(s) | 968 |
| BstSLI | GKGCMC | 1 cut(s) | 579 |
| BstUI | CGCG | 5 cut(s) | 43, 161, 163, 377, 653 |
| BstV1I | GCAGC | 5 cut(s) | 330, 763, 954, 982, 1056 |
| BstX2I | RGATCY | 3 cut(s) | 108, 481, 736 |
| BstYI | RGATCY | 3 cut(s) | 108, 481, 736 |
| BstZ17I | GTATAC | 1 cut(s) | 151 |
| BsuRI | GGCC | 5 cut(s) | 138, 413, 430, 676, 894 |
| BtgI | CCRYGG | 1 cut(s) | 140 |
| BtsCI | GGATG | 5 cut(s) | 207, 502, 736, 820, 1018 |
| BtsIMutI | CAGTG | 1 cut(s) | 677 |
| Cac8I | GCNNGC | 5 cut(s) | 163, 635, 662, 849, 984 |
| CfoI | GCGC | 4 cut(s) | 163, 165, 348, 379 |
| Cfr10I | RCCGGY | 1 cut(s) | 80 |
| Cfr13I | GGNCC | 5 cut(s) | 137, 294, 411, 428, 772 |
| CseI | GACGC | 2 cut(s) | 145, 714 |
| Csp6I | GTAC | 3 cut(s) | 205, 646, 874 |
| CviAII | CATG | 5 cut(s) | 141, 291, 616, 1048, 1067 |
| CviQI | GTAC | 3 cut(s) | 205, 646, 874 |
| DdeI | CTNAG | 1 cut(s) | 902 |
| DpnI | GATC | 6 cut(s) | 71, 110, 228, 304, 483, 738 |
| DpnII | GATC | 6 cut(s) | 69, 108, 226, 302, 481, 736 |
| DraI | TTTAAA | 1 cut(s) | 16 |
| DraIII | CACNNNGTG | 1 cut(s) | 639 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 197 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 197 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| EciI | GGCGGA | 2 cut(s) | 866, 1001 |
| Eco130I | CCWWGG | 1 cut(s) | 140 |
| Eco31I | GGTCTC | 1 cut(s) | 68 |
| Eco47I | GGWCC | 2 cut(s) | 294, 772 |
| Eco57I | CTGAAG | 1 cut(s) | 701 |
| Eco72I | CACGTG | 1 cut(s) | 394 |
| EcoRI | GAATTC | 2 cut(s) | 358, 1096 |
| EcoRII | CCWGG | 2 cut(s) | 130, 1091 |
| EcoT14I | CCWWGG | 1 cut(s) | 140 |
| ErhI | CCWWGG | 1 cut(s) | 140 |
| FaeI | CATG | 5 cut(s) | 144, 294, 619, 1051, 1070 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 545, 577 |
| FaqI | GGGAC | 2 cut(s) | 33, 153 |
| FatI | CATG | 5 cut(s) | 140, 290, 615, 1047, 1066 |
| FauI | CCCGC | 1 cut(s) | 179 |
| FauNDI | CATATG | 1 cut(s) | 758 |
| FblI | GTMKAC | 1 cut(s) | 150 |
| FokI | GGATG | 5 cut(s) | 194, 509, 743, 827, 1025 |
| FspI | TGCGCA | 1 cut(s) | 347 |
| GlaI | GCGC | 4 cut(s) | 162, 164, 347, 378 |
| GsuI | CTGGAG | 2 cut(s) | 371, 386 |
| HaeIII | GGCC | 5 cut(s) | 138, 413, 430, 676, 894 |
| HapII | CCGG | 4 cut(s) | 81, 408, 689, 895 |
| HgaI | GACGC | 2 cut(s) | 145, 714 |
| HhaI | GCGC | 4 cut(s) | 163, 165, 348, 379 |
| Hin1I | GRCGYC | 2 cut(s) | 156, 725 |
| Hin1II | CATG | 5 cut(s) | 144, 294, 619, 1051, 1070 |
| Hin6I | GCGC | 4 cut(s) | 161, 163, 346, 377 |
| HinP1I | GCGC | 4 cut(s) | 161, 163, 346, 377 |
| HincII | GTYRAC | 1 cut(s) | 261 |
| HindII | GTYRAC | 1 cut(s) | 261 |
| HinfI | GANTC | 1 cut(s) | 451 |
| HpaI | GTTAAC | 1 cut(s) | 261 |
| HpaII | CCGG | 4 cut(s) | 81, 408, 689, 895 |
| HphI | GGTGA | 1 cut(s) | 70 |
| Hpy166II | GTNNAC | 6 cut(s) | 151, 261, 474, 577, 646, 772 |
| Hpy188I | TCNGA | 5 cut(s) | 124, 226, 357, 730, 741 |
| Hpy188III | TCNNGA | 5 cut(s) | 67, 230, 403, 838, 1135 |
| Hpy8I | GTNNAC | 6 cut(s) | 151, 261, 474, 577, 646, 772 |
| Hpy99I | CGWCG | 6 cut(s) | 161, 236, 269, 428, 730, 979 |
| HpyAV | CCTTC | 8 cut(s) | 139, 547, 835, 916, 922, 928, 1025, 1120 |
| HpyCH4III | ACNGT | 3 cut(s) | 471, 534, 713 |
| HpyCH4IV | ACGT | 5 cut(s) | 267, 285, 351, 393, 423 |
| HpyCH4V | TGCA | 5 cut(s) | 7, 577, 660, 970, 1044 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 90, 162, 171, 545, 639, 731, 853, 979 |
| HpyF3I | CTNAG | 1 cut(s) | 902 |
| HpySE526I | ACGT | 5 cut(s) | 267, 285, 351, 393, 423 |
| Hsp92I | GRCGYC | 2 cut(s) | 156, 725 |
| Hsp92II | CATG | 5 cut(s) | 144, 294, 619, 1051, 1070 |
| HspAI | GCGC | 4 cut(s) | 161, 163, 346, 377 |
| KspAI | GTTAAC | 1 cut(s) | 261 |
| Kzo9I | GATC | 6 cut(s) | 69, 108, 226, 302, 481, 736 |
| LmnI | GCTCC | 4 cut(s) | 390, 853, 913, 1063 |
| Lsp1109I | GCAGC | 5 cut(s) | 330, 763, 954, 982, 1056 |
| LweI | GCATC | 4 cut(s) | 226, 487, 577, 721 |
| MaeII | ACGT | 5 cut(s) | 267, 285, 351, 393, 423 |
| MaeIII | GTNAC | 5 cut(s) | 76, 268, 286, 713, 1105 |
| MalI | GATC | 6 cut(s) | 71, 110, 228, 304, 483, 738 |
| MbiI | CCGCTC | 1 cut(s) | 1026 |
| MboI | GATC | 6 cut(s) | 69, 108, 226, 302, 481, 736 |
| MboII | GAAGA | 3 cut(s) | 41, 271, 562 |
| MflI | RGATCY | 3 cut(s) | 108, 481, 736 |
| MhlI | GDGCHC | 1 cut(s) | 579 |
| MluCI | AATT | 5 cut(s) | 20, 358, 505, 1087, 1096 |
| MmeI | TCCRAC | 2 cut(s) | 147, 708 |
| MroXI | GAANNNNTTC | 2 cut(s) | 833, 1130 |
| MseI | TTAA | 3 cut(s) | 15, 260, 798 |
| MspI | CCGG | 4 cut(s) | 81, 408, 689, 895 |
| MspR9I | CCNGG | 4 cut(s) | 132, 409, 690, 1093 |
| MvaI | CCWGG | 2 cut(s) | 132, 1093 |
| MvnI | CGCG | 5 cut(s) | 43, 161, 163, 377, 653 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 90, 162, 171, 545, 639, 731, 853, 979 |
| NciI | CCSGG | 2 cut(s) | 409, 690 |
| NcoI | CCATGG | 1 cut(s) | 140 |
| NdeI | CATATG | 1 cut(s) | 758 |
| NdeII | GATC | 6 cut(s) | 69, 108, 226, 302, 481, 736 |
| NlaIII | CATG | 5 cut(s) | 144, 294, 619, 1051, 1070 |
| NlaIV | GGNNCC | 3 cut(s) | 36, 412, 774 |
| NmeAIII | GCCGAG | 1 cut(s) | 130 |
| NmuCI | GTSAC | 2 cut(s) | 76, 286 |
| NsbI | TGCGCA | 1 cut(s) | 347 |
| PauI | GCGCGC | 1 cut(s) | 161 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 432 |
| PdmI | GAANNNNTTC | 2 cut(s) | 833, 1130 |
| PfeI | GAWTC | 1 cut(s) | 451 |
| PflFI | GACNNNGTC | 1 cut(s) | 568 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 58 |
| PfoI | TCCNGGA | 2 cut(s) | 688, 1091 |
| PmaCI | CACGTG | 1 cut(s) | 394 |
| PmlI | CACGTG | 1 cut(s) | 394 |
| Ppu21I | YACGTR | 1 cut(s) | 394 |
| Psp1406I | AACGTT | 1 cut(s) | 351 |
| Psp6I | CCWGG | 2 cut(s) | 130, 1091 |
| PspCI | CACGTG | 1 cut(s) | 394 |
| PspGI | CCWGG | 2 cut(s) | 130, 1091 |
| PspN4I | GGNNCC | 3 cut(s) | 36, 412, 774 |
| PspPI | GGNCC | 5 cut(s) | 137, 294, 411, 428, 772 |
| PstI | CTGCAG | 1 cut(s) | 972 |
| PsuI | RGATCY | 3 cut(s) | 108, 481, 736 |
| PsyI | GACNNNGTC | 1 cut(s) | 568 |
| PteI | GCGCGC | 1 cut(s) | 161 |
| RsaI | GTAC | 3 cut(s) | 206, 647, 875 |
| RsaNI | GTAC | 3 cut(s) | 205, 646, 874 |
| SaqAI | TTAA | 3 cut(s) | 15, 260, 798 |
| Sau3AI | GATC | 6 cut(s) | 69, 108, 226, 302, 481, 736 |
| Sau96I | GGNCC | 5 cut(s) | 137, 294, 411, 428, 772 |
| ScrFI | CCNGG | 4 cut(s) | 132, 409, 690, 1093 |
| SduI | GDGCHC | 1 cut(s) | 579 |
| SfaNI | GCATC | 4 cut(s) | 226, 487, 577, 721 |
| SfcI | CTRYAG | 1 cut(s) | 968 |
| SinI | GGWCC | 2 cut(s) | 294, 772 |
| SmlI | CTYRAG | 1 cut(s) | 521 |
| SmoI | CTYRAG | 1 cut(s) | 521 |
| Sse9I | AATT | 5 cut(s) | 20, 358, 505, 1087, 1096 |
| StyD4I | CCNGG | 4 cut(s) | 130, 407, 688, 1091 |
| StyI | CCWWGG | 1 cut(s) | 140 |
| TaaI | ACNGT | 3 cut(s) | 471, 534, 713 |
| TaiI | ACGT | 5 cut(s) | 270, 288, 354, 396, 426 |
| TaqI | TCGA | 3 cut(s) | 24, 201, 435 |
| TasI | AATT | 5 cut(s) | 20, 358, 505, 1087, 1096 |
| TatI | WGTACW | 1 cut(s) | 204 |
| TauI | GCSGC | 6 cut(s) | 43, 138, 548, 653, 704, 767 |
| TfiI | GAWTC | 1 cut(s) | 451 |
| Tru1I | TTAA | 3 cut(s) | 15, 260, 798 |
| Tru9I | TTAA | 3 cut(s) | 15, 260, 798 |
| TscAI | CASTG | 1 cut(s) | 684 |
| TseFI | GTSAC | 2 cut(s) | 76, 286 |
| TseI | GCWGC | 5 cut(s) | 343, 751, 967, 970, 1044 |
| Tsp45I | GTSAC | 2 cut(s) | 76, 286 |
| TspDTI | ATGAA | 6 cut(s) | 42, 635, 804, 935, 1064, 1139 |
| TspGWI | ACGGA | 3 cut(s) | 246, 352, 491 |
| TspRI | CASTG | 1 cut(s) | 684 |
| Tth111I | GACNNNGTC | 1 cut(s) | 568 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 58 |
| VneI | GTGCAC | 1 cut(s) | 575 |
| VpaK11BI | GGWCC | 2 cut(s) | 294, 772 |
| XapI | RAATTY | 5 cut(s) | 20, 358, 505, 1087, 1096 |
| XmiI | GTMKAC | 1 cut(s) | 150 |
| XmnI | GAANNNNTTC | 2 cut(s) | 833, 1130 |