RchiOBHm_Chr6g0309171

3-oxo-Delta(4,5)-steroid 5-beta-reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000626
Physical Location & Seq
Forward (+)
67029045 .. 67030672
1628 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27801

Sequence Viewer

Length: 1158 bp
ATGAGCTGGTGGCGGGCTGGAGCTGTTGGCGCTGTTAGGAAATTAACAGAAGATGACACCGAAAGTGTTGCGCTAGTGATCGGTGTGACGGGCATAGTAGGCAACAGCCTAGCTGAGATTCTTCCACTCGCGGACACGCCCGGCGGTCCATGGAAGGTGTACGGTGTTGCACGTCGTCCGCGCCCAAATTGGAATGCTGACCATCCCGTTGACTATATCCAGTGCGATATCTCAGACCCAGAAGACACCAAAACCAAGCTCTCCCGGTTAATCGATGTCACACACATCTTCTACGTGACATGGACCAGTAAAGCCACTGAGATTGAGAATTGCGAGGCCAATGGTGCTATGCTCCGCAACGTGCTACGTGTCGTTATCCCAGCGGCTCCAAATCTCCGCCACATCAGCCTCCAAACTGGAACCAAACACTATGTTGGGCCGTTCGAATCTCTTGGTAAGATCCAAGCCCATGATCAGCCTTTCACGGAGGAGTTGCCTAGACTTGATGTGCCCAATTTCTACTACACCCTAGAGGACATCCTTTTCGAAGAGGCTAACAAGAAAGAGGGCCTGACTTGGTCGGTGCACCGGCCCAATCATATATTCGGGTTTTCCCCGTATAGCTTGATGAACATAATTGGCACTCTTTGTGTTTATGCTTCAATATGTAAGCACGAGGGGAAACCACTGCTATTTCCAGGCAGCAAAGAAGCTTGGGAATATTACGCGGTGGCTTCGGATGCAGATCTGATTGCGGAGCAGCATATATGGGCCGCGGTGGATCCAAATGCCAAAAACGAAGCCTTTAACATCAACAATGGGGATGTGTTTAGGTGGAAGAAACTTTGGAAGGTGTTGGCTGAGGAATTTGGGATTGAGGAGTATGGTTTTGAGGAGAAGGGTGAGAAGTTGAGCTTGGTGGATGAGATGAAGGACAAAAGTGGTGTGTGGGAAGAGATAGTGAGGAAGAATCAACTGCAGCCCACCAAATTGCAGGAGGTTGGGGTTTGGTGGTTTGCTGACCTTGTTTTGGGTATGATGCCCGTGTTGGACAGTATGAATAAGAGCAAAGAGCATGGCTTTTTGGGGTTCAGGAATTCCGAAAAGTCATTCGCATCGTGGATTGAAAAAATGAAAGCTCACAAGATTGTGCCGTAG

Protein Analysis

385

Amino Acids

43.61

Weight (kDa)

5.55

Isoelectric Point (pI)

46.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 24 - 271 6.2e-07 NAD dependent epimerase/dehydratase family
PRISE PF22917 82 - 385 1.4e-29 PRISE-like Rossmann-fold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000626)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24220 AT4G24220
fragaria_vesca FvH4_2g04580 FvH4_2g04580 FvH4_2g04580 FvH4_4g06071 FvH4_5g25130
malus_domestica MD05G1094500.v1.1 MD05G1108300.v1.1 MD08G1088400.v1.1 MD15G1073200.v1.1 MD15G1073300.v1.1 MD15G1073400.v1.1 MD15G1073500.v1.1 MD15G1073600.v1.1
prunus_persica Prupe.1G426100_v2.0.a1 Prupe.1G426100_v2.0.a1 Prupe.1G426200_v2.0.a1 Prupe.1G426300_v2.0.a1 Prupe.1G426400_v2.0.a1 Prupe.1G426400_v2.0.a1 Prupe.8G139400_v2.0.a1 Prupe.8G139400_v2.0.a1
pyrus_communis pycom05g09350 pycom08g07190 pycom13g22780 pycom15g06840 pycom15g06850 pycom15g06870
rosa_chinensis RchiOBHm_Chr6g0254161 RchiOBHm_Chr6g0254181 RchiOBHm_Chr6g0254191 RchiOBHm_Chr6g0309171 RchiOBHm_Chr6g0309181 RchiOBHm_Chr6g0309191 RchiOBHm_Chr6g0309201
rosa_laevigata RLG00000010582 RLG00000010583 RLG00000014937 RLG00000014938
rosa_multiflora Rmu_co8060540.1_g000001 Rmu_co8332663.1_g000001 Rmu_sc0000010.1_g000017 Rmu_sc0003711.1_g000008 Rmu_sc0016971.1_g000003 Rmu_sc0020102.1_g000006 Rmu_sc0020102.1_g000008
rosa_roxburghii Rroxscaffold_7G00159570 Rroxscaffold_7G00159580 Rroxscaffold_7G00159590 Rroxscaffold_7G00211100
rosa_rugosa Rorug05G0550400 Rorug06G0370100 Rorug06G0370300
rosa_samantha Rh6AG066900 Rh6AG483200 Rh6AG483300 Rh6AG483400 Rh6BG059800 Rh6BG060000 Rh6BG492200 Rh6BG492300 Rh6BG492500 Rh6CG059700 Rh6CG059800 Rh6CG496900 Rh6CG497000 Rh6CG497200 Rh6CG497400 Rh6DG056700 Rh6DG056800 Rh6DG483800 Rh6DG484000
rosa_wichuraiana Rw6G005860 Rw6G005870 Rw6G042070 Rw6G042080 Rw6G042090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 131, 181, 728, 776
AclWI GGATC 3 cut(s) 454, 776, 789
AcsI RAATTY 2 cut(s) 866, 1096
AfaI GTAC 1 cut(s) 161
AfiI CCNNNNNNNGG 1 cut(s) 1030
AflIII ACRYGT 1 cut(s) 367
AgsI TTSAA 2 cut(s) 663, 1127
AjiI CACGTC 1 cut(s) 173
AjnI CCWGG 1 cut(s) 697
AjuI GAANNNNNNNTTGG 4 cut(s) 587, 619, 899, 931
AluBI AGCT 8 cut(s) 6, 23, 113, 259, 624, 713, 915, 1139
AluI AGCT 8 cut(s) 6, 23, 113, 259, 624, 713, 915, 1139
Alw21I GWGCWC 1 cut(s) 588
Alw44I GTGCAC 1 cut(s) 584
AlwI GGATC 3 cut(s) 454, 776, 789
AoxI GGCC 5 cut(s) 336, 437, 568, 590, 771
ApaLI GTGCAC 1 cut(s) 584
ApeKI GCWGC 3 cut(s) 702, 760, 979
ApoI RAATTY 2 cut(s) 866, 1096
ArsI GACNNNNNNTTYG 2 cut(s) 527, 559
AspLEI GCGC 3 cut(s) 32, 73, 183
AspS9I GGNCC 6 cut(s) 146, 303, 437, 568, 591, 771
AsuC2I CCSGG 2 cut(s) 141, 265
AsuHPI GGTGA 1 cut(s) 914
AsuII TTCGAA 2 cut(s) 444, 546
AvaII GGWCC 2 cut(s) 146, 303
BaeGI GKGCMC 2 cut(s) 513, 588
BamHI GGATCC 1 cut(s) 781
BauI CACGAG 1 cut(s) 674
BbsI GAAGAC 1 cut(s) 249
Bbv12I GWGCWC 1 cut(s) 588
BbvCI CCTCAGC 1 cut(s) 861
BbvI GCAGC 3 cut(s) 714, 772, 991
BccI CCATC 1 cut(s) 210
BceAI ACGGC 2 cut(s) 424, 1138
BcgI CGANNNNNNTGC 2 cut(s) 50, 84
BciT130I CCWGG 1 cut(s) 699
BclI TGATCA 1 cut(s) 472
BcnI CCSGG 2 cut(s) 141, 265
BfaI CTAG 4 cut(s) 74, 110, 498, 530
BfmI CTRYAG 1 cut(s) 977
BfoI RGCGCY 1 cut(s) 33
BglII AGATCT 1 cut(s) 745
BisI GCNGC 5 cut(s) 384, 703, 761, 774, 980
BlsI GCNGC 5 cut(s) 385, 704, 762, 775, 981
Bme1390I CCNGG 3 cut(s) 141, 265, 699
Bme18I GGWCC 2 cut(s) 146, 303
BmgBI CACGTC 1 cut(s) 173
BmgT120I GGNCC 6 cut(s) 146, 303, 437, 568, 591, 771
BmiI GGNNCC 3 cut(s) 387, 421, 783
BmrFI CCNGG 3 cut(s) 141, 265, 699
BmsI GCATC 3 cut(s) 730, 1029, 1124
BpiI GAAGAC 1 cut(s) 249
BpmI CTGGAG 1 cut(s) 39
Bpu10I CCTNAGC 1 cut(s) 861
Bpu14I TTCGAA 2 cut(s) 444, 546
BpuMI CCSGG 2 cut(s) 141, 265
Bsa29I ATCGAT 1 cut(s) 273
BsaAI YACGTR 2 cut(s) 295, 368
BsaBI GATNNNNATC 1 cut(s) 744
BsaJI CCNNGG 2 cut(s) 149, 774
Bsc4I CCNNNNNNNGG 1 cut(s) 1030
Bse118I RCCGGY 1 cut(s) 588
Bse1I ACTGG 3 cut(s) 220, 306, 421
Bse8I GATNNNNATC 1 cut(s) 744
BseBI CCWGG 1 cut(s) 699
BseCI ATCGAT 1 cut(s) 273
BseDI CCNNGG 2 cut(s) 149, 774
BseGI GGATG 5 cut(s) 202, 537, 745, 829, 928
BseJI GATNNNNATC 1 cut(s) 744
BseLI CCNNNNNNNGG 1 cut(s) 1030
BseMII CTCAG 4 cut(s) 105, 246, 309, 852
BseNI ACTGG 3 cut(s) 220, 306, 421
BseRI GAGGAG 3 cut(s) 503, 893, 908
BseSI GKGCMC 2 cut(s) 513, 588
BseXI GCAGC 3 cut(s) 714, 772, 991
BseYI CCCAGC 1 cut(s) 379
Bsh1236I CGCG 4 cut(s) 131, 181, 728, 776
BshFI GGCC 5 cut(s) 338, 439, 570, 592, 773
BshVI ATCGAT 1 cut(s) 273
BsiHKAI GWGCWC 1 cut(s) 588
BsiSI CCGG 3 cut(s) 141, 265, 589
BslI CCNNNNNNNGG 1 cut(s) 1030
BsmI GAATGC 1 cut(s) 199
BsnI GGCC 5 cut(s) 338, 439, 570, 592, 773
Bsp119I TTCGAA 2 cut(s) 444, 546
Bsp1286I GDGCHC 2 cut(s) 513, 588
Bsp143I GATC 5 cut(s) 78, 459, 472, 745, 781
Bsp19I CCATGG 1 cut(s) 149
BspANI GGCC 5 cut(s) 338, 439, 570, 592, 773
BspCNI CTCAG 4 cut(s) 106, 245, 310, 853
BspDI ATCGAT 1 cut(s) 273
BspFNI CGCG 4 cut(s) 131, 181, 728, 776
BspLI GGNNCC 3 cut(s) 387, 421, 783
BspMAI CTGCAG 1 cut(s) 981
BspPI GGATC 3 cut(s) 454, 776, 789
BspT104I TTCGAA 2 cut(s) 444, 546
BsrFI RCCGGY 1 cut(s) 588
BsrI ACTGG 3 cut(s) 220, 306, 421
BssAI RCCGGY 1 cut(s) 588
BssECI CCNNGG 2 cut(s) 149, 774
BssMI GATC 5 cut(s) 78, 459, 472, 745, 781
BssSI CACGAG 1 cut(s) 674
BssT1I CCWWGG 1 cut(s) 149
Bst2BI CACGAG 1 cut(s) 674
Bst2UI CCWGG 1 cut(s) 699
Bst4CI ACNGT 2 cut(s) 164, 1055
Bst6I CTCTTC 2 cut(s) 543, 948
BstBAI YACGTR 2 cut(s) 295, 368
BstBI TTCGAA 2 cut(s) 444, 546
BstC8I GCNNGC 1 cut(s) 15
BstDEI CTNAG 4 cut(s) 114, 232, 318, 861
BstDSI CCRYGG 2 cut(s) 149, 774
BstF5I GGATG 5 cut(s) 202, 537, 745, 829, 928
BstFNI CGCG 4 cut(s) 131, 181, 728, 776
BstH2I RGCGCY 1 cut(s) 33
BstHHI GCGC 3 cut(s) 32, 73, 183
BstKTI GATC 5 cut(s) 81, 462, 475, 748, 784
BstMBI GATC 5 cut(s) 78, 459, 472, 745, 781
BstMWI GCNNNNNNNGC 5 cut(s) 29, 99, 344, 405, 740
BstNI CCWGG 1 cut(s) 699
BstSCI CCNGG 3 cut(s) 139, 263, 697
BstSFI CTRYAG 1 cut(s) 977
BstSLI GKGCMC 2 cut(s) 513, 588
BstUI CGCG 4 cut(s) 131, 181, 728, 776
BstV1I GCAGC 3 cut(s) 714, 772, 991
BstV2I GAAGAC 1 cut(s) 249
BstX2I RGATCY 3 cut(s) 459, 745, 781
BstYI RGATCY 3 cut(s) 459, 745, 781
Bsu15I ATCGAT 1 cut(s) 273
BsuRI GGCC 5 cut(s) 338, 439, 570, 592, 773
BsuTUI ATCGAT 1 cut(s) 273
BtgI CCRYGG 2 cut(s) 149, 774
BtrI CACGTC 1 cut(s) 173
BtsCI GGATG 5 cut(s) 202, 537, 745, 829, 928
BtsI GCAGTG 1 cut(s) 686
BtsIMutI CAGTG 3 cut(s) 227, 315, 686
Cac8I GCNNGC 1 cut(s) 15
CfoI GCGC 3 cut(s) 32, 73, 183
Cfr10I RCCGGY 1 cut(s) 588
Cfr13I GGNCC 6 cut(s) 146, 303, 437, 568, 591, 771
Cfr42I CCGCGG 1 cut(s) 777
ClaI ATCGAT 1 cut(s) 273
Csp6I GTAC 1 cut(s) 160
CspCI CAANNNNNGTGG 2 cut(s) 304, 339
CviAII CATG 4 cut(s) 150, 300, 470, 1076
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 4 cut(s) 114, 232, 318, 861
DpnI GATC 5 cut(s) 80, 461, 474, 747, 783
DpnII GATC 5 cut(s) 78, 459, 472, 745, 781
Eam1104I CTCTTC 2 cut(s) 543, 948
EarI CTCTTC 2 cut(s) 543, 948
EciI GGCGGA 1 cut(s) 386
Eco130I CCWWGG 1 cut(s) 149
Eco32I GATATC 1 cut(s) 229
Eco47I GGWCC 2 cut(s) 146, 303
EcoO109I RGGNCCY 1 cut(s) 568
EcoRI GAATTC 1 cut(s) 1096
EcoRII CCWGG 1 cut(s) 697
EcoRV GATATC 1 cut(s) 229
EcoT14I CCWWGG 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 149
FaeI CATG 4 cut(s) 153, 303, 473, 1079
FalI AAGNNNNNCTT 2 cut(s) 899, 931
FatI CATG 4 cut(s) 149, 299, 469, 1075
FauI CCCGC 1 cut(s) 6
FbaI TGATCA 1 cut(s) 472
Fnu4HI GCNGC 5 cut(s) 384, 703, 761, 774, 980
FokI GGATG 5 cut(s) 189, 524, 752, 836, 935
Fsp4HI GCNGC 5 cut(s) 384, 703, 761, 774, 980
FspBI CTAG 4 cut(s) 74, 110, 498, 530
GlaI GCGC 3 cut(s) 31, 72, 182
GluI GCNGC 5 cut(s) 384, 703, 761, 774, 980
GsaI CCCAGC 1 cut(s) 383
GsuI CTGGAG 1 cut(s) 39
HaeII RGCGCY 1 cut(s) 33
HaeIII GGCC 5 cut(s) 338, 439, 570, 592, 773
HapII CCGG 3 cut(s) 141, 265, 589
HhaI GCGC 3 cut(s) 32, 73, 183
Hin1II CATG 4 cut(s) 153, 303, 473, 1079
Hin6I GCGC 3 cut(s) 30, 71, 181
HinP1I GCGC 3 cut(s) 30, 71, 181
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HindIII AAGCTT 1 cut(s) 711
HinfI GANTC 3 cut(s) 118, 446, 970
HpaII CCGG 3 cut(s) 141, 265, 589
HphI GGTGA 1 cut(s) 914
Hpy166II GTNNAC 3 cut(s) 160, 211, 586
Hpy188I TCNGA 4 cut(s) 235, 739, 750, 1102
Hpy188III TCNNGA 1 cut(s) 1093
Hpy8I GTNNAC 3 cut(s) 160, 211, 586
Hpy99I CGWCG 1 cut(s) 177
HpyAV CCTTC 4 cut(s) 148, 844, 892, 925
HpyCH4III ACNGT 2 cut(s) 164, 1055
HpyCH4IV ACGT 4 cut(s) 172, 294, 360, 367
HpyCH4V TGCA 5 cut(s) 170, 586, 743, 979, 994
HpyF10VI GCNNNNNNNGC 5 cut(s) 29, 99, 344, 405, 740
HpyF3I CTNAG 4 cut(s) 114, 232, 318, 861
HpySE526I ACGT 4 cut(s) 172, 294, 360, 367
Hsp92II CATG 4 cut(s) 153, 303, 473, 1079
HspAI GCGC 3 cut(s) 30, 71, 181
Ksp22I TGATCA 1 cut(s) 472
KspI CCGCGG 1 cut(s) 777
Kzo9I GATC 5 cut(s) 78, 459, 472, 745, 781
LmnI GCTCC 4 cut(s) 20, 357, 391, 757
Lsp1109I GCAGC 3 cut(s) 714, 772, 991
LweI GCATC 3 cut(s) 730, 1029, 1124
MaeI CTAG 4 cut(s) 74, 110, 498, 530
MaeII ACGT 4 cut(s) 172, 294, 360, 367
MaeIII GTNAC 3 cut(s) 85, 277, 295
MalI GATC 5 cut(s) 80, 461, 474, 747, 783
MboI GATC 5 cut(s) 78, 459, 472, 745, 781
MboII GAAGA 8 cut(s) 62, 113, 254, 280, 560, 850, 965, 979
MflI RGATCY 3 cut(s) 459, 745, 781
MhlI GDGCHC 2 cut(s) 513, 588
MluCI AATT 8 cut(s) 41, 187, 328, 514, 636, 866, 989, 1096
MmeI TCCRAC 1 cut(s) 1029
MseI TTAA 3 cut(s) 44, 269, 807
MspA1I CMGCKG 2 cut(s) 383, 776
MspI CCGG 3 cut(s) 141, 265, 589
MspR9I CCNGG 3 cut(s) 141, 265, 699
Mva1269I GAATGC 1 cut(s) 199
MvaI CCWGG 1 cut(s) 699
MvnI CGCG 4 cut(s) 131, 181, 728, 776
MwoI GCNNNNNNNGC 5 cut(s) 29, 99, 344, 405, 740
NciI CCSGG 2 cut(s) 141, 265
NcoI CCATGG 1 cut(s) 149
NdeII GATC 5 cut(s) 78, 459, 472, 745, 781
NlaIII CATG 4 cut(s) 153, 303, 473, 1079
NlaIV GGNNCC 3 cut(s) 387, 421, 783
NmuCI GTSAC 3 cut(s) 85, 277, 295
NspV TTCGAA 2 cut(s) 444, 546
PctI GAATGC 1 cut(s) 199
PfeI GAWTC 3 cut(s) 118, 446, 970
PflFI GACNNNGTC 1 cut(s) 577
PkrI GCNGC 5 cut(s) 385, 704, 762, 775, 981
Ppu21I YACGTR 2 cut(s) 295, 368
Psp6I CCWGG 1 cut(s) 697
PspFI CCCAGC 1 cut(s) 379
PspGI CCWGG 1 cut(s) 697
PspN4I GGNNCC 3 cut(s) 387, 421, 783
PspPI GGNCC 6 cut(s) 146, 303, 437, 568, 591, 771
PstI CTGCAG 1 cut(s) 981
PsuI RGATCY 3 cut(s) 459, 745, 781
PsyI GACNNNGTC 1 cut(s) 577
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
SacII CCGCGG 1 cut(s) 777
SaqAI TTAA 3 cut(s) 44, 269, 807
SatI GCNGC 5 cut(s) 384, 703, 761, 774, 980
Sau3AI GATC 5 cut(s) 78, 459, 472, 745, 781
Sau96I GGNCC 6 cut(s) 146, 303, 437, 568, 591, 771
ScrFI CCNGG 3 cut(s) 141, 265, 699
SduI GDGCHC 2 cut(s) 513, 588
SfaNI GCATC 3 cut(s) 730, 1029, 1124
SfcI CTRYAG 1 cut(s) 977
Sfr303I CCGCGG 1 cut(s) 777
SfuI TTCGAA 2 cut(s) 444, 546
SgrBI CCGCGG 1 cut(s) 777
SinI GGWCC 2 cut(s) 146, 303
Sse9I AATT 8 cut(s) 41, 187, 328, 514, 636, 866, 989, 1096
SspI AATATT 1 cut(s) 722
SspMI CTAG 4 cut(s) 74, 110, 498, 530
StyD4I CCNGG 3 cut(s) 139, 263, 697
StyI CCWWGG 1 cut(s) 149
TaaI ACNGT 2 cut(s) 164, 1055
TaiI ACGT 4 cut(s) 175, 297, 363, 370
TaqI TCGA 3 cut(s) 273, 444, 546
TasI AATT 8 cut(s) 41, 187, 328, 514, 636, 866, 989, 1096
TauI GCSGC 2 cut(s) 386, 776
TfiI GAWTC 3 cut(s) 118, 446, 970
Tru1I TTAA 3 cut(s) 44, 269, 807
Tru9I TTAA 3 cut(s) 44, 269, 807
TscAI CASTG 3 cut(s) 227, 322, 693
TseFI GTSAC 3 cut(s) 85, 277, 295
TseI GCWGC 3 cut(s) 702, 760, 979
Tsp45I GTSAC 3 cut(s) 85, 277, 295
TspDTI ATGAA 4 cut(s) 644, 944, 1073, 1148
TspGWI ACGGA 1 cut(s) 500
TspRI CASTG 3 cut(s) 227, 322, 693
Tth111I GACNNNGTC 1 cut(s) 577
VneI GTGCAC 1 cut(s) 584
VpaK11BI GGWCC 2 cut(s) 146, 303
XapI RAATTY 2 cut(s) 866, 1096
XspI CTAG 4 cut(s) 74, 110, 498, 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.