AT5G43155

QWRF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001505
Physical Location & Seq
Forward (+)
17328460 .. 17329240
781 bp
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UTR
Exon/CDS
Intron
AT5G43155.1

Sequence Viewer

Length: 717 bp
ATGACAGCTGCAACAATTTCACCTTCTTTCAACGCAAACGTTAAACAGAACAAACCACCGTCGTTTCCATCGGAATCATCAAATCGCCGCCCAAAAACACGAGATGTAGCATCACGATACCTTGGCGGTACTTCTTCTTTCTTCCACCAATCATCACCGAAGAGATGTCAATCTCCGATCGTTACTCGACCGGTGACTCCGTCGTCTGTTGCTACTAATCGGCCGCAATCAACACCACGTCGTGAGTCTTTAGATCGACGAGAAGTTTCTAAAGCGGAGAGGATGTTGTTGACTTCTGGGAGAAGCTTGTTCGCGTCGTTTCAGGCGGATTCGTTTACTCCAGGAACATTAGAGAGGAGGAAGACGACTTCTTCGGCAACAATATCTAAGAGTGGTGGAGGTAAACAAGAGAAATTGAAACTCTCTGATCAATGGCCTAGGTCTCTGCAACCTAGTTGTTTAAGTAGTAGAAGTGTAGATTTCACTGATACTAGGAAGAAATTGATCGGATCTGGCAATGGTGTAGCTAGAGCTCTGCAGGATTCCATGGTTAGTAATAGACCGGTTTCGCGTGAGCGGATAACGAGTGTTGATTTGGAGACAGAGAGTGTTTCTTCTGGAAGCTCTAATGGAAGAGGGAAAATGTTACCAGCTCGTGGCAATGTGGTTAAGGCGAGAGTTTCGCAGGATCGGAAGCCAATTGTTTCCAAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

238

Amino Acids

25.95

Weight (kDa)

11.35

Isoelectric Point (pI)

66.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 202
AccB7I CCANNNNNTGG 1 cut(s) 656
AccBSI CCGCTC 1 cut(s) 577
AccII CGCG 2 cut(s) 314, 571
AciI CCGC 6 cut(s) 88, 126, 224, 275, 326, 577
AclI AACGTT 1 cut(s) 39
AclWI GGATC 2 cut(s) 517, 696
AcoI YGGCCR 1 cut(s) 221
AdeI CACNNNGTG 1 cut(s) 242
AfaI GTAC 1 cut(s) 130
AfiI CCNNNNNNNGG 1 cut(s) 656
AgeI ACCGGT 2 cut(s) 190, 562
AgsI TTSAA 2 cut(s) 31, 418
AjiI CACGTC 1 cut(s) 239
AjnI CCWGG 1 cut(s) 340
AluBI AGCT 6 cut(s) 8, 306, 527, 533, 624, 653
AluI AGCT 6 cut(s) 8, 306, 527, 533, 624, 653
Alw21I GWGCWC 1 cut(s) 535
Alw26I GTCTC 2 cut(s) 447, 593
AlwI GGATC 2 cut(s) 517, 696
AoxI GGCC 2 cut(s) 221, 434
ApeKI GCWGC 1 cut(s) 8
ArsI GACNNNNNNTTYG 2 cut(s) 355, 387
AsiGI ACCGGT 2 cut(s) 190, 562
AspA2I CCTAGG 1 cut(s) 437
AsuHPI GGTGA 3 cut(s) 12, 147, 205
AvrII CCTAGG 1 cut(s) 437
BanII GRGCYC 1 cut(s) 535
BauI CACGAG 2 cut(s) 99, 654
BbsI GAAGAC 1 cut(s) 368
Bbv12I GWGCWC 1 cut(s) 535
BccI CCATC 1 cut(s) 76
BciT130I CCWGG 1 cut(s) 342
BclI TGATCA 1 cut(s) 427
BcoDI GTCTC 2 cut(s) 447, 593
BfaI CTAG 4 cut(s) 438, 453, 492, 528
BfmI CTRYAG 1 cut(s) 536
BisI GCNGC 3 cut(s) 9, 88, 224
BlnI CCTAGG 1 cut(s) 437
BlsI GCNGC 3 cut(s) 10, 89, 225
Bme1390I CCNGG 1 cut(s) 342
BmgBI CACGTC 1 cut(s) 239
BmrFI CCNGG 1 cut(s) 342
BmsI GCATC 1 cut(s) 119
BpiI GAAGAC 1 cut(s) 368
BpmI CTGGAG 1 cut(s) 324
BsaBI GATNNNNATC 1 cut(s) 169
BsaI GGTCTC 1 cut(s) 447
BsaJI CCNNGG 3 cut(s) 121, 437, 546
BsaWI WCCGGW 2 cut(s) 190, 562
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 1 cut(s) 656
Bse118I RCCGGY 2 cut(s) 190, 562
Bse3DI GCAATG 2 cut(s) 523, 667
Bse8I GATNNNNATC 1 cut(s) 169
BseBI CCWGG 1 cut(s) 342
BseDI CCNNGG 3 cut(s) 121, 437, 546
BseGI GGATG 1 cut(s) 288
BseJI GATNNNNATC 1 cut(s) 169
BseLI CCNNNNNNNGG 1 cut(s) 656
BseMI GCAATG 2 cut(s) 523, 667
BseRI GAGGAG 1 cut(s) 370
BseX3I CGGCCG 1 cut(s) 221
Bsh1236I CGCG 2 cut(s) 314, 571
Bsh1285I CGRYCG 3 cut(s) 180, 191, 224
BshFI GGCC 2 cut(s) 223, 436
BshTI ACCGGT 2 cut(s) 190, 562
BsiEI CGRYCG 3 cut(s) 180, 191, 224
BsiHKAI GWGCWC 1 cut(s) 535
BsiSI CCGG 2 cut(s) 191, 563
BslI CCNNNNNNNGG 1 cut(s) 656
BsmAI GTCTC 2 cut(s) 447, 593
BsnI GGCC 2 cut(s) 223, 436
Bso31I GGTCTC 1 cut(s) 447
Bsp1286I GDGCHC 1 cut(s) 535
Bsp143I GATC 6 cut(s) 177, 253, 427, 504, 509, 688
Bsp19I CCATGG 1 cut(s) 546
BspACI CCGC 6 cut(s) 88, 126, 224, 275, 326, 577
BspANI GGCC 2 cut(s) 223, 436
BspFNI CGCG 2 cut(s) 314, 571
BspMAI CTGCAG 1 cut(s) 540
BspPI GGATC 2 cut(s) 517, 696
BspTNI GGTCTC 1 cut(s) 447
BsrBI CCGCTC 1 cut(s) 577
BsrDI GCAATG 2 cut(s) 523, 667
BsrFI RCCGGY 2 cut(s) 190, 562
BssAI RCCGGY 2 cut(s) 190, 562
BssECI CCNNGG 3 cut(s) 121, 437, 546
BssMI GATC 6 cut(s) 177, 253, 427, 504, 509, 688
BssSI CACGAG 2 cut(s) 99, 654
BssT1I CCWWGG 3 cut(s) 121, 437, 546
Bst2BI CACGAG 2 cut(s) 99, 654
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 1 cut(s) 60
Bst6I CTCTTC 2 cut(s) 155, 628
BstDEI CTNAG 1 cut(s) 387
BstDSI CCRYGG 1 cut(s) 546
BstF5I GGATG 1 cut(s) 288
BstFNI CGCG 2 cut(s) 314, 571
BstKTI GATC 6 cut(s) 180, 256, 430, 507, 512, 691
BstMAI GTCTC 2 cut(s) 447, 593
BstMBI GATC 6 cut(s) 177, 253, 427, 504, 509, 688
BstMCI CGRYCG 3 cut(s) 180, 191, 224
BstNI CCWGG 1 cut(s) 342
BstSCI CCNGG 1 cut(s) 340
BstSFI CTRYAG 1 cut(s) 536
BstUI CGCG 2 cut(s) 314, 571
BstV2I GAAGAC 1 cut(s) 368
BstX2I RGATCY 1 cut(s) 509
BstYI RGATCY 1 cut(s) 509
BstZI CGGCCG 1 cut(s) 221
BsuRI GGCC 2 cut(s) 223, 436
BtgI CCRYGG 1 cut(s) 546
BtrI CACGTC 1 cut(s) 239
BtsCI GGATG 1 cut(s) 288
BtsIMutI CAGTG 1 cut(s) 483
Cfr10I RCCGGY 2 cut(s) 190, 562
CseI GACGC 1 cut(s) 303
Csp6I GTAC 1 cut(s) 129
CspAI ACCGGT 2 cut(s) 190, 562
CviAII CATG 1 cut(s) 547
CviJI RGCY 9 cut(s) 8, 223, 306, 436, 527, 533, 624, 653, 697
CviKI_1 RGCY 9 cut(s) 8, 223, 306, 436, 527, 533, 624, 653, 697
CviQI GTAC 1 cut(s) 129
DdeI CTNAG 1 cut(s) 387
DpnI GATC 6 cut(s) 179, 255, 429, 506, 511, 690
DpnII GATC 6 cut(s) 177, 253, 427, 504, 509, 688
DraIII CACNNNGTG 1 cut(s) 242
DrdI GACNNNNNNGTC 1 cut(s) 202
DseDI GACNNNNNNGTC 1 cut(s) 202
EaeI YGGCCR 1 cut(s) 221
EagI CGGCCG 1 cut(s) 221
Eam1104I CTCTTC 2 cut(s) 155, 628
EarI CTCTTC 2 cut(s) 155, 628
EciI GGCGGA 1 cut(s) 341
Ecl136II GAGCTC 1 cut(s) 533
EclXI CGGCCG 1 cut(s) 221
Eco130I CCWWGG 3 cut(s) 121, 437, 546
Eco24I GRGCYC 1 cut(s) 535
Eco31I GGTCTC 1 cut(s) 447
Eco52I CGGCCG 1 cut(s) 221
Eco53kI GAGCTC 1 cut(s) 533
EcoICRI GAGCTC 1 cut(s) 533
EcoRII CCWGG 1 cut(s) 340
EcoT14I CCWWGG 3 cut(s) 121, 437, 546
EcoT38I GRGCYC 1 cut(s) 535
ErhI CCWWGG 3 cut(s) 121, 437, 546
FaeI CATG 1 cut(s) 550
FaiI YATR 1 cut(s) 548
FatI CATG 1 cut(s) 546
FbaI TGATCA 1 cut(s) 427
Fnu4HI GCNGC 3 cut(s) 9, 88, 224
FokI GGATG 1 cut(s) 295
FriOI GRGCYC 1 cut(s) 535
Fsp4HI GCNGC 3 cut(s) 9, 88, 224
FspBI CTAG 4 cut(s) 438, 453, 492, 528
GluI GCNGC 3 cut(s) 9, 88, 224
GsuI CTGGAG 1 cut(s) 324
HaeIII GGCC 2 cut(s) 223, 436
HapII CCGG 2 cut(s) 191, 563
HgaI GACGC 1 cut(s) 303
Hin1II CATG 1 cut(s) 550
HincII GTYRAC 1 cut(s) 291
HindII GTYRAC 1 cut(s) 291
HindIII AAGCTT 1 cut(s) 304
HinfI GANTC 5 cut(s) 74, 196, 245, 329, 542
HpaII CCGG 2 cut(s) 191, 563
HphI GGTGA 3 cut(s) 12, 147, 205
Hpy166II GTNNAC 3 cut(s) 291, 336, 404
Hpy188I TCNGA 5 cut(s) 73, 177, 427, 509, 693
Hpy188III TCNNGA 3 cut(s) 114, 242, 618
Hpy8I GTNNAC 3 cut(s) 291, 336, 404
Hpy99I CGWCG 5 cut(s) 64, 205, 243, 261, 319
HpyAV CCTTC 1 cut(s) 33
HpyCH4III ACNGT 1 cut(s) 60
HpyCH4IV ACGT 2 cut(s) 39, 238
HpyCH4V TGCA 3 cut(s) 11, 448, 538
HpyF3I CTNAG 1 cut(s) 387
HpySE526I ACGT 2 cut(s) 39, 238
Hsp92II CATG 1 cut(s) 550
Ksp22I TGATCA 1 cut(s) 427
Kzo9I GATC 6 cut(s) 177, 253, 427, 504, 509, 688
LweI GCATC 1 cut(s) 119
MaeI CTAG 4 cut(s) 438, 453, 492, 528
MaeII ACGT 2 cut(s) 39, 238
MaeIII GTNAC 3 cut(s) 181, 193, 645
MalI GATC 6 cut(s) 179, 255, 429, 506, 511, 690
MbiI CCGCTC 1 cut(s) 577
MboI GATC 6 cut(s) 177, 253, 427, 504, 509, 688
MboII GAAGA 8 cut(s) 126, 133, 172, 363, 373, 508, 606, 645
MfeI CAATTG 1 cut(s) 699
MflI RGATCY 1 cut(s) 509
MhlI GDGCHC 1 cut(s) 535
MluCI AATT 4 cut(s) 15, 413, 500, 699
MlyI GAGTC 2 cut(s) 190, 254
MnlI CCTC 5 cut(s) 273, 348, 351, 392, 629
MseI TTAA 3 cut(s) 42, 461, 669
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 2 cut(s) 191, 563
MspR9I CCNGG 1 cut(s) 342
MunI CAATTG 1 cut(s) 699
MvaI CCWGG 1 cut(s) 342
MvnI CGCG 2 cut(s) 314, 571
NcoI CCATGG 1 cut(s) 546
NdeII GATC 6 cut(s) 177, 253, 427, 504, 509, 688
NlaIII CATG 1 cut(s) 550
NmuCI GTSAC 1 cut(s) 193
PfeI GAWTC 3 cut(s) 74, 329, 542
PflFI GACNNNGTC 1 cut(s) 199
PflMI CCANNNNNTGG 1 cut(s) 656
PfoI TCCNGGA 1 cut(s) 340
PinAI ACCGGT 2 cut(s) 190, 562
PkrI GCNGC 3 cut(s) 10, 89, 225
Ple19I CGATCG 1 cut(s) 180
PleI GAGTC 2 cut(s) 190, 253
PpsI GAGTC 2 cut(s) 190, 253
Psp124BI GAGCTC 1 cut(s) 535
Psp1406I AACGTT 1 cut(s) 39
Psp6I CCWGG 1 cut(s) 340
PspGI CCWGG 1 cut(s) 340
PstI CTGCAG 1 cut(s) 540
PsuI RGATCY 1 cut(s) 509
PsyI GACNNNGTC 1 cut(s) 199
PvuI CGATCG 1 cut(s) 180
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 1 cut(s) 130
RsaNI GTAC 1 cut(s) 129
SacI GAGCTC 1 cut(s) 535
SaqAI TTAA 3 cut(s) 42, 461, 669
SatI GCNGC 3 cut(s) 9, 88, 224
Sau3AI GATC 6 cut(s) 177, 253, 427, 504, 509, 688
SchI GAGTC 2 cut(s) 190, 254
ScrFI CCNGG 1 cut(s) 342
SduI GDGCHC 1 cut(s) 535
SfaNI GCATC 1 cut(s) 119
SfcI CTRYAG 1 cut(s) 536
Sse9I AATT 4 cut(s) 15, 413, 500, 699
SsiI CCGC 6 cut(s) 88, 126, 224, 275, 326, 577
SspMI CTAG 4 cut(s) 438, 453, 492, 528
SstI GAGCTC 1 cut(s) 535
StyD4I CCNGG 1 cut(s) 340
StyI CCWWGG 3 cut(s) 121, 437, 546
TaaI ACNGT 1 cut(s) 60
TaiI ACGT 2 cut(s) 42, 241
TaqI TCGA 2 cut(s) 187, 256
TasI AATT 4 cut(s) 15, 413, 500, 699
TauI GCSGC 2 cut(s) 90, 226
TfiI GAWTC 3 cut(s) 74, 329, 542
Tru1I TTAA 3 cut(s) 42, 461, 669
Tru9I TTAA 3 cut(s) 42, 461, 669
TscAI CASTG 1 cut(s) 490
TseFI GTSAC 1 cut(s) 193
TseI GCWGC 1 cut(s) 8
Tsp45I GTSAC 1 cut(s) 193
TspGWI ACGGA 1 cut(s) 189
TspRI CASTG 1 cut(s) 490
Tth111I GACNNNGTC 1 cut(s) 199
Van91I CCANNNNNTGG 1 cut(s) 656
XmaJI CCTAGG 1 cut(s) 437
XspI CTAG 4 cut(s) 438, 453, 492, 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.