Rw1G000140

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001505
Physical Location & Seq
Reverse (-)
187442 .. 190842
3401 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G000140.1

Sequence Viewer

Length: 549 bp
ATGCCTTCGCTCGCCTCTACTCCGCCGAGGACAGCGGTCACTTGTCGACCTAATTCTTTGGATTTGAGGGCCCGGAATGGAGCTGTCGGTGGCTCAGAAGTTGTTGGTCACTTCGACGAGGACTTTATCGAAACAACTTCAAAACTCTGGGAATCTGTTAGAGCCAAAAGAACTGAGTTACAATTGCTGAGGCAAAATTTGAAGCTAACTTCCATTCTCCAGGGGCAAGTAGATCAAGTGTTGGTTATGGACCGGGATTACTCTAATTCCTTGTCTGGTGCCATCGAAGCTTTGAGGGCTAGCACACTCCGCCTTCTAGTGGTTGATGGAGCAAGGGCAGATGTCCACAATGTGAAGGATGTTATTTCTTCCGCAGTTGATGTGATGCAGGCAATGGCATCATCAATATGCTTATTATTATCGAAGGCCACTAGCCAAGTCACCGTTAACCTCAGGTCACTGGATGCAAGTCACTGGCAAATTATAAACTCCAGCTTACTAAATCAATTAGTTTTTTGTATCTTTTTATGGTGTCACTGTTATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

182

Amino Acids

20.01

Weight (kDa)

6.89

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 44 - 144 6.5e-33 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 485
AccB1I GGYRCC 1 cut(s) 278
AccI GTMKAC 1 cut(s) 46
AciI CCGC 4 cut(s) 23, 35, 310, 372
AcsI RAATTY 1 cut(s) 196
AdeI CACNNNGTG 1 cut(s) 352
AfiI CCNNNNNNNGG 1 cut(s) 319
AgsI TTSAA 2 cut(s) 141, 202
AjnI CCWGG 1 cut(s) 219
AluBI AGCT 4 cut(s) 83, 205, 290, 495
AluI AGCT 4 cut(s) 83, 205, 290, 495
AoxI GGCC 2 cut(s) 69, 426
ApaI GGGCCC 1 cut(s) 73
ApoI RAATTY 1 cut(s) 196
AspS9I GGNCC 3 cut(s) 69, 70, 250
AsuC2I CCSGG 2 cut(s) 73, 254
AsuHPI GGTGA 1 cut(s) 433
AsuNHI GCTAGC 1 cut(s) 299
AvaII GGWCC 1 cut(s) 250
AxyI CCTNAGG 1 cut(s) 452
BaeGI GKGCMC 1 cut(s) 73
BanI GGYRCC 1 cut(s) 278
BanII GRGCYC 1 cut(s) 73
BbvCI CCTCAGC 1 cut(s) 188
BccI CCATC 2 cut(s) 290, 320
BciT130I CCWGG 1 cut(s) 221
BcnI CCSGG 2 cut(s) 73, 254
BfaI CTAG 3 cut(s) 300, 317, 432
Bme1390I CCNGG 3 cut(s) 73, 221, 254
Bme18I GGWCC 1 cut(s) 250
BmgT120I GGNCC 3 cut(s) 69, 70, 250
BmiI GGNNCC 2 cut(s) 71, 280
BmrFI CCNGG 3 cut(s) 73, 221, 254
BmsI GCATC 3 cut(s) 375, 407, 454
BmtI GCTAGC 1 cut(s) 303
BoxI GACNNNNGTC 1 cut(s) 35
BpmI CTGGAG 2 cut(s) 203, 475
Bpu10I CCTNAGC 1 cut(s) 188
BpuMI CCSGG 2 cut(s) 73, 254
BsaJI CCNNGG 2 cut(s) 26, 220
Bsc4I CCNNNNNNNGG 1 cut(s) 319
Bse1I ACTGG 2 cut(s) 465, 479
Bse21I CCTNAGG 1 cut(s) 452
Bse3DI GCAATG 1 cut(s) 399
BseBI CCWGG 1 cut(s) 221
BseDI CCNNGG 2 cut(s) 26, 220
BseGI GGATG 2 cut(s) 364, 469
BseLI CCNNNNNNNGG 1 cut(s) 319
BseMI GCAATG 1 cut(s) 399
BseMII CTCAG 4 cut(s) 108, 165, 179, 466
BseNI ACTGG 2 cut(s) 465, 479
BseSI GKGCMC 1 cut(s) 73
BshFI GGCC 2 cut(s) 71, 428
BshNI GGYRCC 1 cut(s) 278
BsiSI CCGG 2 cut(s) 73, 253
BslI CCNNNNNNNGG 1 cut(s) 319
BsnI GGCC 2 cut(s) 71, 428
Bsp120I GGGCCC 1 cut(s) 69
Bsp1286I GDGCHC 1 cut(s) 73
Bsp143I GATC 1 cut(s) 232
BspACI CCGC 4 cut(s) 23, 35, 310, 372
BspANI GGCC 2 cut(s) 71, 428
BspCNI CTCAG 4 cut(s) 107, 166, 180, 465
BspLI GGNNCC 2 cut(s) 71, 280
BspOI GCTAGC 1 cut(s) 303
BspT107I GGYRCC 1 cut(s) 278
BsrDI GCAATG 1 cut(s) 399
BsrI ACTGG 2 cut(s) 465, 479
BssECI CCNNGG 2 cut(s) 26, 220
BssMI GATC 1 cut(s) 232
Bst2UI CCWGG 1 cut(s) 221
Bst4CI ACNGT 2 cut(s) 445, 539
BstC8I GCNNGC 3 cut(s) 12, 301, 390
BstDEI CTNAG 4 cut(s) 94, 174, 188, 452
BstF5I GGATG 2 cut(s) 364, 469
BstKTI GATC 1 cut(s) 235
BstMBI GATC 1 cut(s) 232
BstMWI GCNNNNNNNGC 3 cut(s) 287, 296, 309
BstNI CCWGG 1 cut(s) 221
BstPAI GACNNNNGTC 1 cut(s) 35
BstSCI CCNGG 3 cut(s) 71, 219, 252
BstSLI GKGCMC 1 cut(s) 73
Bsu36I CCTNAGG 1 cut(s) 452
BsuRI GGCC 2 cut(s) 71, 428
BtsCI GGATG 2 cut(s) 364, 469
BtsIMutI CAGTG 3 cut(s) 458, 472, 535
Cac8I GCNNGC 3 cut(s) 12, 301, 390
Cfr13I GGNCC 3 cut(s) 69, 70, 250
DdeI CTNAG 4 cut(s) 94, 174, 188, 452
DpnI GATC 1 cut(s) 234
DpnII GATC 1 cut(s) 232
DraIII CACNNNGTG 1 cut(s) 352
EciI GGCGGA 2 cut(s) 12, 299
Eco24I GRGCYC 1 cut(s) 73
Eco47I GGWCC 1 cut(s) 250
Eco81I CCTNAGG 1 cut(s) 452
EcoO109I RGGNCCY 1 cut(s) 69
EcoRII CCWGG 1 cut(s) 219
EcoT38I GRGCYC 1 cut(s) 73
FaiI YATR 5 cut(s) 248, 409, 485, 529, 543
FblI GTMKAC 1 cut(s) 46
FokI GGATG 2 cut(s) 371, 476
FriOI GRGCYC 1 cut(s) 73
FspBI CTAG 3 cut(s) 300, 317, 432
GsuI CTGGAG 2 cut(s) 203, 475
HaeIII GGCC 2 cut(s) 71, 428
HapII CCGG 2 cut(s) 73, 253
HincII GTYRAC 2 cut(s) 47, 448
HindII GTYRAC 2 cut(s) 47, 448
HindIII AAGCTT 1 cut(s) 288
HinfI GANTC 1 cut(s) 152
HpaI GTTAAC 1 cut(s) 448
HpaII CCGG 2 cut(s) 73, 253
HphI GGTGA 1 cut(s) 433
Hpy166II GTNNAC 3 cut(s) 47, 346, 448
Hpy188I TCNGA 1 cut(s) 97
Hpy8I GTNNAC 3 cut(s) 47, 346, 448
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 4 cut(s) 15, 323, 349, 418
HpyCH4III ACNGT 2 cut(s) 445, 539
HpyCH4V TGCA 2 cut(s) 388, 467
HpyF10VI GCNNNNNNNGC 3 cut(s) 287, 296, 309
HpyF3I CTNAG 4 cut(s) 94, 174, 188, 452
KspAI GTTAAC 1 cut(s) 448
Kzo9I GATC 1 cut(s) 232
LmnI GCTCC 2 cut(s) 80, 329
LweI GCATC 3 cut(s) 375, 407, 454
MaeI CTAG 3 cut(s) 300, 317, 432
MaeIII GTNAC 7 cut(s) 37, 107, 177, 439, 456, 470, 533
MalI GATC 1 cut(s) 234
MboI GATC 1 cut(s) 232
MboII GAAGA 1 cut(s) 360
MfeI CAATTG 1 cut(s) 182
MhlI GDGCHC 1 cut(s) 73
MluCI AATT 6 cut(s) 52, 182, 196, 265, 480, 506
MnlI CCTC 7 cut(s) 21, 25, 60, 112, 183, 288, 461
MseI TTAA 1 cut(s) 447
MslI CAYNNNNRTG 2 cut(s) 406, 540
MspA1I CMGCKG 1 cut(s) 35
MspI CCGG 2 cut(s) 73, 253
MspR9I CCNGG 3 cut(s) 73, 221, 254
MunI CAATTG 1 cut(s) 182
MvaI CCWGG 1 cut(s) 221
MwoI GCNNNNNNNGC 3 cut(s) 287, 296, 309
NciI CCSGG 2 cut(s) 73, 254
NdeII GATC 1 cut(s) 232
NheI GCTAGC 1 cut(s) 299
NlaIV GGNNCC 2 cut(s) 71, 280
NmeAIII GCCGAG 1 cut(s) 51
NmuCI GTSAC 6 cut(s) 37, 107, 439, 456, 470, 533
PfeI GAWTC 1 cut(s) 152
PshAI GACNNNNGTC 1 cut(s) 35
PsiI TTATAA 1 cut(s) 485
Psp6I CCWGG 1 cut(s) 219
PspGI CCWGG 1 cut(s) 219
PspN4I GGNNCC 2 cut(s) 71, 280
PspOMI GGGCCC 1 cut(s) 69
PspPI GGNCC 3 cut(s) 69, 70, 250
RseI CAYNNNNRTG 2 cut(s) 406, 540
SalI GTCGAC 1 cut(s) 45
SaqAI TTAA 1 cut(s) 447
Sau3AI GATC 1 cut(s) 232
Sau96I GGNCC 3 cut(s) 69, 70, 250
ScrFI CCNGG 3 cut(s) 73, 221, 254
SduI GDGCHC 1 cut(s) 73
SetI ASST 7 cut(s) 52, 85, 207, 292, 453, 458, 497
SfaNI GCATC 3 cut(s) 375, 407, 454
SinI GGWCC 1 cut(s) 250
SmiMI CAYNNNNRTG 2 cut(s) 406, 540
Sse9I AATT 6 cut(s) 52, 182, 196, 265, 480, 506
SsiI CCGC 4 cut(s) 23, 35, 310, 372
SspMI CTAG 3 cut(s) 300, 317, 432
StyD4I CCNGG 3 cut(s) 71, 219, 252
TaaI ACNGT 2 cut(s) 445, 539
TaqI TCGA 5 cut(s) 46, 114, 129, 285, 422
TasI AATT 6 cut(s) 52, 182, 196, 265, 480, 506
TfiI GAWTC 1 cut(s) 152
Tru1I TTAA 1 cut(s) 447
Tru9I TTAA 1 cut(s) 447
TscAI CASTG 3 cut(s) 465, 479, 542
TseFI GTSAC 6 cut(s) 37, 107, 439, 456, 470, 533
Tsp45I GTSAC 6 cut(s) 37, 107, 439, 456, 470, 533
TspRI CASTG 3 cut(s) 465, 479, 542
VpaK11BI GGWCC 1 cut(s) 250
XapI RAATTY 1 cut(s) 196
XmiI GTMKAC 1 cut(s) 46
XspI CTAG 3 cut(s) 300, 317, 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.