MD08G1086900.v1.1

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001505
Physical Location & Seq
Reverse (-)
7219414 .. 7224737
5324 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1086900.v1.1.491

Sequence Viewer

Length: 1947 bp
ATGGGATTTTGTGTTTCTGCGATGGTAGCTGCTGTGTCGACGACATTGAACCCCAAGGCCGCGTCCCAAGGAGGAGGTCGTCCCCAAAACGCCACCGTAAGGCCTCCTCTTCTGCCTTCTGAACCCGACAATGGCTTCGCCGTTCCTCGCCGCCTCAAAGCTCGGGAGGTCACTTCCCGGTACATGTCCTCCTCATCTTCTACTTCTTCCTCTACTTGTTCGAATTCGAGGCGTTGTCCCTCTCCGTTGCCGTCCAGAACCGGCGGTTCGACGGCGGCGACGCCGTTGTCGCAGTCTGTGAAGCGCTCGCAATCGGTGGAGAGGAGGAGAGCTGTCACTCCTCGACCCAATTCTTTGAATTTGAGGACCGGGAATGTTGGTGCTGGAGGCGGTGAAATGTCCGCGGCTCAGAAGCTACTGTTCACTTCAACGAGGAGCTTATCGGTGTCGTTTCAGGGCGAGTCGTACTCGCTCCAGGTGCGTAAAGCGAAACCGGCGCCGTCTCCGAGTACAAGGAAAGGCACTCCGGAGAGGAGGAAGGCGACGACGCCGTTTGGAGCAGATCAGAGTGAGAATTCCAAGCCGACAGAGCAGCAGCGGTGGCCTGCGAGATTGCGACAACCGAATAGTATGAGTAGGAGCTTGGATTGCACGGATGAGAGGAAGAGGATGAGTGGATCTGGCGCCAATGTGGTTAGAGCATTACAAAGCTCAATGGTGGATGATGTTGATGGGAGATTGAGGTTGAATTCCTGCAATTTAGTCTCAGGGAAAGCTACTGAGGCTGTTGATGGGGGCAATTCAGGTAATACACAGTCCGAGACCGAGACTTGGTCGAATTTTGATAGTGTGTCTTTTGGCAGTGCTAATTCAGGACCGCACGAGCTTGGTAATGGCTGTGGTGGCGGCACCAATGGCTACGTTGGTGTTGGACAAGCACCACGGCCTAGAGGGATTGTTGTGCCTGCAAGGTTTTGGCAAGAGACTAACAGTAGATTGCGCCGCCAATCAGAGTCTAATAAGGGTAATGCAACCAAAAATATGGGTTCTCCTAAACATATTGAGAGCAACAGGTTATCTATGGATAGTCCAGTGTCATCTCCTCGAGGTGTGGTGAATAGCAGGGGGCAGTTATCCCCTATTCGTGGAGCTGCTGGGCCTGCTTCGCCTAGTAAGCTCAGCAGGTCGTTGGTGCCATCTTCGCCTATGAGGGGATTGAGCCCTTCTCGGATGAGGAATGGAGTGTTGGCTACTCCAAGTAGTAATTTGAGCATGCCTTCAGTTTTAAGTTTCGCTGCTGATGTTCGAAGAGGGAAGGTTGGAGAGAACCGGATGGTTGATGCACATGTGGTGAGGCTATTACATAACCGGCTCTTGCAGTGGCGCTTTGTAAATGCAAGAGCTGATGCCACCCTAGCTGCACAGAGGTCCAATGCAGAGGGAAGCCTCTATAATGCATGGGTAACGAGTTCAAGACTTTGTGAATCTGTAAGACAAAAACGGATTGAGTTACATCTGCTGAGGCAACATGTGAAGATAACATCCATCCTAAAGGGGCAAATAACATATCTGGAAGAGTTGTCACTTATGGATCGGGATTACTCGGATTCTTTGTCAGGGGCCATTGAAGCTTTGAAAGCTAGCACGCTCCGCCTTCCAGTTGTTGGAGCAAGGGCTGATGTCTACAATGTGAAGGATGCTATTTCTTCAGCAGTTGATGTGATGCAAGCGATGGCATCATCAATATGTTTATTGTTATCGAAGGCTGGGGATGTGAACTTTTTGGTGGCTGAACTTGCAAATATAACTGCGAAGGAGCATGCTTTGCTTGGTCAGTGCAGAGATCTATTGTCAACAGTTGCAGCCATGCAGGTGGAGGAATGTAGCCTCAGGACGCATATTTTACAACAGAAAAGCGTACCTGACAGCCTCACGGCGGAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

649

Amino Acids

69.31

Weight (kDa)

10.39

Isoelectric Point (pI)

62.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 312 - 610 2.3e-102 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1861
AasI GACNNNNNNGTC 1 cut(s) 286
AbsI CCTCGAGG 1 cut(s) 1104
Acc36I ACCTGC 2 cut(s) 1173, 1861
AccB1I GGYRCC 4 cut(s) 496, 683, 908, 1192
AccB7I CCANNNNNTGG 1 cut(s) 1664
AccI GTMKAC 2 cut(s) 38, 1683
AccII CGCG 2 cut(s) 62, 404
AccIII TCCGGA 1 cut(s) 526
AclWI GGATC 2 cut(s) 685, 1599
AcsI RAATTY 5 cut(s) 223, 358, 574, 748, 838
AcuI CTGAAG 2 cut(s) 1263, 1692
AcyI GRCGYC 4 cut(s) 281, 497, 548, 684
AfaI GTAC 4 cut(s) 182, 467, 511, 1920
AfeI AGCGCT 1 cut(s) 305
AfiI CCNNNNNNNGG 7 cut(s) 99, 131, 831, 1145, 1211, 1664, 1936
AflIII ACRYGT 3 cut(s) 183, 1345, 1528
AgsI TTSAA 7 cut(s) 49, 358, 429, 748, 1473, 1628, 1636
AjnI CCWGG 1 cut(s) 474
AjuI GAANNNNNNNTTGG 2 cut(s) 1229, 1261
Alw26I GTCTC 5 cut(s) 507, 769, 815, 821, 977
AlwI GGATC 2 cut(s) 685, 1599
Ama87I CYCGRG 2 cut(s) 162, 1104
Aor13HI TCCGGA 1 cut(s) 526
Aor51HI AGCGCT 1 cut(s) 305
AoxI GGCC 6 cut(s) 57, 101, 602, 944, 1157, 1620
ApeKI GCWGC 7 cut(s) 29, 592, 595, 1151, 1295, 1418, 1862
ApoI RAATTY 5 cut(s) 223, 358, 574, 748, 838
ArsI GACNNNNNNTTYG 2 cut(s) 119, 151
AspLEI GCGC 5 cut(s) 306, 499, 686, 1002, 1386
AspS9I GGNCC 5 cut(s) 366, 875, 1157, 1428, 1620
AsuC2I CCSGG 2 cut(s) 178, 370
AsuHPI GGTGA 3 cut(s) 404, 1126, 1363
AsuII TTCGAA 2 cut(s) 221, 1306
AsuNHI GCTAGC 1 cut(s) 1640
AvaI CYCGRG 2 cut(s) 162, 1104
AvaII GGWCC 3 cut(s) 366, 875, 1428
AxyI CCTNAGG 1 cut(s) 1889
BanI GGYRCC 4 cut(s) 496, 683, 908, 1192
BanII GRGCYC 1 cut(s) 1223
BauI CACGAG 1 cut(s) 881
BbvCI CCTCAGC 1 cut(s) 1520
BbvI GCAGC 7 cut(s) 16, 604, 607, 1138, 1282, 1405, 1874
BccI CCATC 7 cut(s) 16, 725, 785, 1204, 1327, 1553, 1726
BceAI ACGGC 7 cut(s) 125, 235, 268, 288, 484, 535, 959
BciT130I CCWGG 1 cut(s) 476
BcnI CCSGG 2 cut(s) 178, 370
BcoDI GTCTC 5 cut(s) 507, 769, 815, 821, 977
BfaI CTAG 4 cut(s) 948, 1170, 1415, 1641
BfoI RGCGCY 4 cut(s) 307, 500, 687, 1387
BfuAI ACCTGC 2 cut(s) 1173, 1861
BglII AGATCT 1 cut(s) 1843
BlpI GCTNAGC 1 cut(s) 1178
Bme1390I CCNGG 3 cut(s) 178, 370, 476
Bme18I GGWCC 3 cut(s) 366, 875, 1428
BmeT110I CYCGRG 2 cut(s) 162, 1104
BmgT120I GGNCC 5 cut(s) 366, 875, 1157, 1428, 1620
BmiI GGNNCC 5 cut(s) 498, 685, 910, 1194, 1621
BmrFI CCNGG 3 cut(s) 178, 370, 476
BmsI GCATC 5 cut(s) 1330, 1396, 1687, 1713, 1745
BmtI GCTAGC 1 cut(s) 1644
BplI GAGNNNNNCTC 4 cut(s) 452, 484, 1210, 1242
BpmI CTGGAG 2 cut(s) 405, 458
Bpu10I CCTNAGC 1 cut(s) 1520
Bpu1102I GCTNAGC 1 cut(s) 1178
Bpu14I TTCGAA 2 cut(s) 221, 1306
BpuMI CCSGG 2 cut(s) 178, 370
BsaHI GRCGYC 4 cut(s) 281, 497, 548, 684
BsaI GGTCTC 1 cut(s) 815
BsaJI CCNNGG 4 cut(s) 54, 67, 402, 941
BsaWI WCCGGW 2 cut(s) 526, 1329
BsaXI ACNNNNNCTCC 2 cut(s) 173, 203
Bsc4I CCNNNNNNNGG 7 cut(s) 99, 131, 831, 1145, 1211, 1664, 1936
Bse118I RCCGGY 3 cut(s) 260, 493, 1368
Bse1I ACTGG 2 cut(s) 1091, 1658
Bse21I CCTNAGG 1 cut(s) 1889
BseAI TCCGGA 1 cut(s) 526
BseBI CCWGG 1 cut(s) 476
BseDI CCNNGG 4 cut(s) 54, 67, 402, 941
BseGI GGATG 9 cut(s) 661, 675, 727, 1236, 1338, 1541, 1545, 1702, 1777
BseLI CCNNNNNNNGG 7 cut(s) 99, 131, 831, 1145, 1211, 1664, 1936
BseMII CTCAG 6 cut(s) 422, 771, 780, 1192, 1511, 1903
BseNI ACTGG 2 cut(s) 1091, 1658
BseRI GAGGAG 9 cut(s) 87, 96, 181, 330, 337, 340, 448, 547, 1092
BseXI GCAGC 7 cut(s) 16, 604, 607, 1138, 1282, 1405, 1874
BseYI CCCAGC 2 cut(s) 1154, 1766
BsgI GTGCAG 2 cut(s) 1404, 1858
Bsh1236I CGCG 2 cut(s) 62, 404
BshFI GGCC 6 cut(s) 59, 103, 604, 946, 1159, 1622
BshNI GGYRCC 4 cut(s) 496, 683, 908, 1192
BsiHKCI CYCGRG 2 cut(s) 162, 1104
BsiSI CCGG 7 cut(s) 178, 261, 369, 494, 527, 1330, 1369
BslFI GGGAC 3 cut(s) 49, 66, 222
BslI CCNNNNNNNGG 7 cut(s) 99, 131, 831, 1145, 1211, 1664, 1936
BsmAI GTCTC 5 cut(s) 507, 769, 815, 821, 977
BsmBI CGTCTC 1 cut(s) 507
BsmFI GGGAC 3 cut(s) 49, 66, 222
BsnI GGCC 6 cut(s) 59, 103, 604, 946, 1159, 1622
Bso31I GGTCTC 1 cut(s) 815
BsoBI CYCGRG 2 cut(s) 162, 1104
Bsp119I TTCGAA 2 cut(s) 221, 1306
Bsp1286I GDGCHC 1 cut(s) 1223
Bsp13I TCCGGA 1 cut(s) 526
Bsp143I GATC 4 cut(s) 562, 677, 1591, 1843
Bsp1720I GCTNAGC 1 cut(s) 1178
BspANI GGCC 6 cut(s) 59, 103, 604, 946, 1159, 1622
BspCNI CTCAG 6 cut(s) 421, 772, 779, 1191, 1512, 1902
BspEI TCCGGA 1 cut(s) 526
BspFNI CGCG 2 cut(s) 62, 404
BspLI GGNNCC 5 cut(s) 498, 685, 910, 1194, 1621
BspMI ACCTGC 2 cut(s) 1173, 1861
BspOI GCTAGC 1 cut(s) 1644
BspPI GGATC 2 cut(s) 685, 1599
BspT104I TTCGAA 2 cut(s) 221, 1306
BspT107I GGYRCC 4 cut(s) 496, 683, 908, 1192
BspTNI GGTCTC 1 cut(s) 815
BsrFI RCCGGY 3 cut(s) 260, 493, 1368
BsrI ACTGG 2 cut(s) 1091, 1658
BssAI RCCGGY 3 cut(s) 260, 493, 1368
BssECI CCNNGG 4 cut(s) 54, 67, 402, 941
BssMI GATC 4 cut(s) 562, 677, 1591, 1843
BssNI GRCGYC 4 cut(s) 281, 497, 548, 684
BssSI CACGAG 1 cut(s) 881
BssT1I CCWWGG 2 cut(s) 54, 67
Bst2BI CACGAG 1 cut(s) 881
Bst2UI CCWGG 1 cut(s) 476
Bst4CI ACNGT 5 cut(s) 97, 420, 816, 992, 1858
Bst6I CTCTTC 4 cut(s) 114, 659, 1303, 1569
BstACI GRCGYC 4 cut(s) 281, 497, 548, 684
BstAPI GCANNNNNTGC 1 cut(s) 1825
BstBI TTCGAA 2 cut(s) 221, 1306
BstC8I GCNNGC 9 cut(s) 308, 606, 966, 1161, 1274, 1642, 1646, 1728, 1821
BstDEI CTNAG 6 cut(s) 408, 766, 780, 1178, 1520, 1889
BstDSI CCRYGG 2 cut(s) 402, 941
BstF5I GGATG 9 cut(s) 661, 675, 727, 1236, 1338, 1541, 1545, 1702, 1777
BstFNI CGCG 2 cut(s) 62, 404
BstH2I RGCGCY 4 cut(s) 307, 500, 687, 1387
BstHHI GCGC 5 cut(s) 306, 499, 686, 1002, 1386
BstKTI GATC 4 cut(s) 565, 680, 1594, 1846
BstMAI GTCTC 5 cut(s) 507, 769, 815, 821, 977
BstMBI GATC 4 cut(s) 562, 677, 1591, 1843
BstNI CCWGG 1 cut(s) 476
BstNSI RCATGY 5 cut(s) 187, 1276, 1349, 1532, 1823
BstSCI CCNGG 3 cut(s) 176, 368, 474
BstUI CGCG 2 cut(s) 62, 404
BstV1I GCAGC 7 cut(s) 16, 604, 607, 1138, 1282, 1405, 1874
BstX2I RGATCY 2 cut(s) 677, 1843
BstXI CCANNNNNNTGG 2 cut(s) 1042, 1873
BstYI RGATCY 2 cut(s) 677, 1843
Bsu36I CCTNAGG 1 cut(s) 1889
BsuRI GGCC 6 cut(s) 59, 103, 604, 946, 1159, 1622
BtgI CCRYGG 2 cut(s) 402, 941
BtgZI GCGATG 2 cut(s) 35, 1745
BtsCI GGATG 9 cut(s) 661, 675, 727, 1236, 1338, 1541, 1545, 1702, 1777
BtsI GCAGTG 2 cut(s) 868, 1385
BtsIMutI CAGTG 4 cut(s) 868, 1098, 1385, 1841
BveI ACCTGC 2 cut(s) 1173, 1861
Cac8I GCNNGC 9 cut(s) 308, 606, 966, 1161, 1274, 1642, 1646, 1728, 1821
CfoI GCGC 5 cut(s) 306, 499, 686, 1002, 1386
Cfr10I RCCGGY 3 cut(s) 260, 493, 1368
Cfr13I GGNCC 5 cut(s) 366, 875, 1157, 1428, 1620
Cfr42I CCGCGG 1 cut(s) 405
CseI GACGC 4 cut(s) 51, 289, 556, 1903
Csp6I GTAC 4 cut(s) 181, 466, 510, 1919
CviAII CATG 7 cut(s) 184, 1273, 1346, 1458, 1529, 1820, 1867
CviQI GTAC 4 cut(s) 181, 466, 510, 1919
DdeI CTNAG 6 cut(s) 408, 766, 780, 1178, 1520, 1889
DinI GGCGCC 2 cut(s) 498, 685
DpnI GATC 4 cut(s) 564, 679, 1593, 1845
DpnII GATC 4 cut(s) 562, 677, 1591, 1843
DrdI GACNNNNNNGTC 1 cut(s) 286
DseDI GACNNNNNNGTC 1 cut(s) 286
Eam1104I CTCTTC 4 cut(s) 114, 659, 1303, 1569
EarI CTCTTC 4 cut(s) 114, 659, 1303, 1569
EciI GGCGGA 1 cut(s) 1640
Eco130I CCWWGG 2 cut(s) 54, 67
Eco147I AGGCCT 1 cut(s) 103
Eco24I GRGCYC 1 cut(s) 1223
Eco31I GGTCTC 1 cut(s) 815
Eco47I GGWCC 3 cut(s) 366, 875, 1428
Eco47III AGCGCT 1 cut(s) 305
Eco57I CTGAAG 2 cut(s) 1263, 1692
Eco81I CCTNAGG 1 cut(s) 1889
Eco88I CYCGRG 2 cut(s) 162, 1104
EcoRI GAATTC 3 cut(s) 223, 574, 748
EcoRII CCWGG 1 cut(s) 474
EcoT14I CCWWGG 2 cut(s) 54, 67
EcoT22I ATGCAT 1 cut(s) 1459
EcoT38I GRGCYC 1 cut(s) 1223
EgeI GGCGCC 2 cut(s) 498, 685
EheI GGCGCC 2 cut(s) 498, 685
ErhI CCWWGG 2 cut(s) 54, 67
Esp3I CGTCTC 1 cut(s) 507
FaeI CATG 7 cut(s) 187, 1276, 1349, 1461, 1532, 1823, 1870
FaqI GGGAC 3 cut(s) 49, 66, 222
FatI CATG 7 cut(s) 183, 1272, 1345, 1457, 1528, 1819, 1866
FblI GTMKAC 2 cut(s) 38, 1683
FokI GGATG 9 cut(s) 668, 682, 734, 1243, 1345, 1528, 1532, 1709, 1784
FriOI GRGCYC 1 cut(s) 1223
FspBI CTAG 4 cut(s) 948, 1170, 1415, 1641
GlaI GCGC 5 cut(s) 305, 498, 685, 1001, 1385
GsaI CCCAGC 2 cut(s) 1158, 1770
GsuI CTGGAG 2 cut(s) 405, 458
HaeII RGCGCY 4 cut(s) 307, 500, 687, 1387
HaeIII GGCC 6 cut(s) 59, 103, 604, 946, 1159, 1622
HapII CCGG 7 cut(s) 178, 261, 369, 494, 527, 1330, 1369
HgaI GACGC 4 cut(s) 51, 289, 556, 1903
HhaI GCGC 5 cut(s) 306, 499, 686, 1002, 1386
Hin1I GRCGYC 4 cut(s) 281, 497, 548, 684
Hin1II CATG 7 cut(s) 187, 1276, 1349, 1461, 1532, 1823, 1870
Hin6I GCGC 5 cut(s) 304, 497, 684, 1000, 1384
HinP1I GCGC 5 cut(s) 304, 497, 684, 1000, 1384
HincII GTYRAC 2 cut(s) 39, 1854
HindII GTYRAC 2 cut(s) 39, 1854
HindIII AAGCTT 1 cut(s) 1629
HinfI GANTC 4 cut(s) 461, 1013, 1484, 1607
HpaII CCGG 7 cut(s) 178, 261, 369, 494, 527, 1330, 1369
HphI GGTGA 3 cut(s) 404, 1126, 1363
Hpy166II GTNNAC 5 cut(s) 39, 423, 1684, 1777, 1854
Hpy188I TCNGA 8 cut(s) 121, 411, 507, 567, 820, 1012, 1230, 1606
Hpy188III TCNNGA 8 cut(s) 164, 255, 527, 873, 1473, 1571, 1595, 1891
Hpy8I GTNNAC 5 cut(s) 39, 423, 1684, 1777, 1854
Hpy99I CGWCG 5 cut(s) 43, 274, 283, 547, 550
HpyAV CCTTC 9 cut(s) 126, 532, 1233, 1287, 1309, 1664, 1687, 1756, 1807
HpyCH4III ACNGT 5 cut(s) 97, 420, 816, 992, 1858
HpyCH4IV ACGT 1 cut(s) 921
HpyF3I CTNAG 6 cut(s) 408, 766, 780, 1178, 1520, 1889
HpySE526I ACGT 1 cut(s) 921
Hsp92I GRCGYC 4 cut(s) 281, 497, 548, 684
Hsp92II CATG 7 cut(s) 187, 1276, 1349, 1461, 1532, 1823, 1870
HspAI GCGC 5 cut(s) 304, 497, 684, 1000, 1384
KasI GGCGCC 2 cut(s) 496, 683
Kpn2I TCCGGA 1 cut(s) 526
KspI CCGCGG 1 cut(s) 405
Kzo9I GATC 4 cut(s) 562, 677, 1591, 1843
LmnI GCTCC 8 cut(s) 435, 477, 557, 639, 1148, 1653, 1667, 1816
Lsp1109I GCAGC 7 cut(s) 16, 604, 607, 1138, 1282, 1405, 1874
LweI GCATC 5 cut(s) 1330, 1396, 1687, 1713, 1745
MaeI CTAG 4 cut(s) 948, 1170, 1415, 1641
MaeII ACGT 1 cut(s) 921
MaeIII GTNAC 5 cut(s) 169, 334, 1462, 1509, 1581
MalI GATC 4 cut(s) 564, 679, 1593, 1845
MboI GATC 4 cut(s) 562, 677, 1591, 1843
MboII GAAGA 9 cut(s) 101, 189, 198, 676, 1191, 1320, 1546, 1586, 1698
MflI RGATCY 2 cut(s) 677, 1843
MhlI GDGCHC 1 cut(s) 1223
Mly113I GGCGCC 2 cut(s) 497, 684
MlyI GAGTC 2 cut(s) 470, 1022
MmeI TCCRAC 3 cut(s) 910, 1300, 1645
Mph1103I ATGCAT 1 cut(s) 1459
MroI TCCGGA 1 cut(s) 526
MseI TTAA 1 cut(s) 1286
MslI CAYNNNNRTG 2 cut(s) 1744, 1871
MspA1I CMGCKG 2 cut(s) 404, 598
MspI CCGG 7 cut(s) 178, 261, 369, 494, 527, 1330, 1369
MspR9I CCNGG 3 cut(s) 178, 370, 476
MvaI CCWGG 1 cut(s) 476
MvnI CGCG 2 cut(s) 62, 404
NarI GGCGCC 2 cut(s) 497, 684
NciI CCSGG 2 cut(s) 178, 370
NdeII GATC 4 cut(s) 562, 677, 1591, 1843
NheI GCTAGC 1 cut(s) 1640
NlaIII CATG 7 cut(s) 187, 1276, 1349, 1461, 1532, 1823, 1870
NlaIV GGNNCC 5 cut(s) 498, 685, 910, 1194, 1621
NmuCI GTSAC 3 cut(s) 169, 334, 1581
NsiI ATGCAT 1 cut(s) 1459
NspI RCATGY 5 cut(s) 187, 1276, 1349, 1532, 1823
NspV TTCGAA 2 cut(s) 221, 1306
PaeI GCATGC 2 cut(s) 1276, 1823
PaeR7I CTCGAG 1 cut(s) 1104
PaqCI CACCTGC 1 cut(s) 1861
PceI AGGCCT 1 cut(s) 103
PciI ACATGT 3 cut(s) 183, 1345, 1528
PcsI WCGNNNNNNNCGW 1 cut(s) 275
PfeI GAWTC 2 cut(s) 1484, 1607
PflFI GACNNNGTC 1 cut(s) 832
PflMI CCANNNNNTGG 1 cut(s) 1664
PleI GAGTC 2 cut(s) 469, 1021
PluTI GGCGCC 2 cut(s) 500, 687
PpsI GAGTC 2 cut(s) 469, 1021
PscI ACATGT 3 cut(s) 183, 1345, 1528
Psp6I CCWGG 1 cut(s) 474
PspFI CCCAGC 2 cut(s) 1154, 1766
PspGI CCWGG 1 cut(s) 474
PspN4I GGNNCC 5 cut(s) 498, 685, 910, 1194, 1621
PspPI GGNCC 5 cut(s) 366, 875, 1157, 1428, 1620
PspXI VCTCGAGB 1 cut(s) 1104
PsuI RGATCY 2 cut(s) 677, 1843
PsyI GACNNNGTC 1 cut(s) 832
RsaI GTAC 4 cut(s) 182, 467, 511, 1920
RsaNI GTAC 4 cut(s) 181, 466, 510, 1919
RseI CAYNNNNRTG 2 cut(s) 1744, 1871
SacII CCGCGG 1 cut(s) 405
SalI GTCGAC 1 cut(s) 37
SaqAI TTAA 1 cut(s) 1286
Sau3AI GATC 4 cut(s) 562, 677, 1591, 1843
Sau96I GGNCC 5 cut(s) 366, 875, 1157, 1428, 1620
SchI GAGTC 2 cut(s) 470, 1022
ScrFI CCNGG 3 cut(s) 178, 370, 476
SduI GDGCHC 1 cut(s) 1223
SfaNI GCATC 5 cut(s) 1330, 1396, 1687, 1713, 1745
SfoI GGCGCC 2 cut(s) 498, 685
Sfr274I CTCGAG 1 cut(s) 1104
Sfr303I CCGCGG 1 cut(s) 405
SfuI TTCGAA 2 cut(s) 221, 1306
SgrBI CCGCGG 1 cut(s) 405
SinI GGWCC 3 cut(s) 366, 875, 1428
SlaI CTCGAG 1 cut(s) 1104
SmiMI CAYNNNNRTG 2 cut(s) 1744, 1871
SmlI CTYRAG 1 cut(s) 1104
SmoI CTYRAG 1 cut(s) 1104
SphI GCATGC 2 cut(s) 1276, 1823
SseBI AGGCCT 1 cut(s) 103
SspDI GGCGCC 2 cut(s) 496, 683
SspMI CTAG 4 cut(s) 948, 1170, 1415, 1641
StuI AGGCCT 1 cut(s) 103
StyD4I CCNGG 3 cut(s) 176, 368, 474
StyI CCWWGG 2 cut(s) 54, 67
TaaI ACNGT 5 cut(s) 97, 420, 816, 992, 1858
TaiI ACGT 1 cut(s) 924
TaqI TCGA 9 cut(s) 38, 221, 227, 269, 343, 836, 1105, 1306, 1760
TaqII GACCGA 1 cut(s) 839
TatI WGTACW 1 cut(s) 509
TauI GCSGC 6 cut(s) 62, 153, 278, 407, 909, 1005
TfiI GAWTC 2 cut(s) 1484, 1607
Tru1I TTAA 1 cut(s) 1286
Tru9I TTAA 1 cut(s) 1286
TscAI CASTG 4 cut(s) 868, 1098, 1385, 1841
TseFI GTSAC 3 cut(s) 169, 334, 1581
TseI GCWGC 7 cut(s) 29, 592, 595, 1151, 1295, 1418, 1862
Tsp45I GTSAC 3 cut(s) 169, 334, 1581
TspGWI ACGGA 3 cut(s) 234, 668, 1516
TspRI CASTG 4 cut(s) 868, 1098, 1385, 1841
Tth111I GACNNNGTC 1 cut(s) 832
Van91I CCANNNNNTGG 1 cut(s) 1664
VpaK11BI GGWCC 3 cut(s) 366, 875, 1428
XapI RAATTY 5 cut(s) 223, 358, 574, 748, 838
XceI RCATGY 5 cut(s) 187, 1276, 1349, 1532, 1823
XhoI CTCGAG 1 cut(s) 1104
XmiI GTMKAC 2 cut(s) 38, 1683
XspI CTAG 4 cut(s) 948, 1170, 1415, 1641
Zsp2I ATGCAT 1 cut(s) 1459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.