MD15G1071600.v1.1

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001505
Physical Location & Seq
Reverse (-)
4918992 .. 4924167
5176 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1071600.v1.1.491

Sequence Viewer

Length: 1932 bp
ATGGTACCTGCTGTGTCGACGACATTGAACCCCAAAGCCGCGTCCCAAGGAGGAGGTCGTCCTCAAAACGGCACCGTAAGGCCGCCTCTTCTGCCTTCTGAATCCGACAATGGCGTCGCTGTTCCTCGCCGCCTCAAAGCTCGGGAGGTCACTTCTCGGTACATGTCCTCCTCCTCCTCTTCTTCTTCCTCAACTTGTTCAAATTCAAGGCGGTGTCCGTCGCCGTTGCCGTCCAGAACTGGCTGCTCGACGGCGGCGACGCCGTTGTCGCAGTCTGTGAAGCGCTCGCAATCGGTGGAGAGGAGGAGAGTTGTCACTCCTCGCCCTAATTCTTTGGATTTGAGGATCGGGAATGGTGGTGCTAGCGGCGGCGGCATGTCCGCGGCTCAAAAGCTACTGTTCACTTCGACGAGGAGCTTATCGGTGTCGTTTCAGGGCGAGTCCTACTCCCTCCAGGTTAGTAAAGCGAAGCCGGCGCCGTCTCCGAATATAAGGAAAGGCACGCCGGAGAGGAGGAAGGTCACGACGCCGTTTAGAGCGGATCAGAGTGAGAATTCAAAGCCGACAGAGCAGCAGCTGTGGCCTGCGAGGTTGCGGCAACCAAATAGTATGAGTAGGAGCCTGGATTGCACGGATGAGAGGAAGAGGGTGAGTGGATCTGGGGCCAATGTGGTTAGGGCATTACAAAACTCGATGGTGGATGATGTTGATGGGAGATTGAGGTCGAATTCTTGCAATTTAGGTTCAGTGAAAGCTACTGAGCCAGTTGATGACGGCAATTCAGCTACTACACAATCTGAGCCTGTGGCTTGTTCCGATTCTGATAGTGTGTCTTCCGACAGTACTAATTCAGGACTACACGAGCTTGGTAATGTCGGTGGTGGTGGCACAAATGGCTACGGCGGTGTTGGACAAGGGCCACGGCCTAGAGGGATGGTGGTCCCTGCGAGGTTTTGGCAAGAGACTAACAATAGATTGCGCCGCCAATCAGAGTCTAATAAGGGTAATGCAACAAAAAATATGGGTTCTCCTAAACAGATTGAGAGCAGCAGGTTATCTATGGATAGTCCAGTGTCATCTCCTCGAGGTGTTGTGAATAGCAGGGGGCAGTTATCCCCCATTCGTGGAGCTGCTCGACCTGCTTCACCAAGTAAGCTCAGCAGGTCGTTGGTGCCATCTTCACCTATGAGGGGAGTGAGCCCTTCCCGGGTGAGGAATGGAGTGGTAGTTACTCCAAGTAGTAGTTTGAGCAACATGCCTTCAGTTTTAAGTTTTGCTGCTGATGTTCGAAGAGGCAAGGTTGGAGAGAACCGGATAGTTGATGCACATGTGGTGAGGCTATTACATAACCGGCTCTTGCAGTGGCGTTTTGTAAATGCTAGAGCTAATGCCGCCCTAGCTGCACAGAGGTCCAATGCAGAGAGAAGCCTCTATAATGCATGGGTAACTAGTTCAAGACTTCGTGAATCTGTAAGACAAAAACGGATCGAGTTACATCTGCTGAGGCAACATTTGAAGCTAACTTCCATCCTAAAGGGACAAATGACATATCTGGAAGAGTTGTCACTTATGGATCAGGATTACTCTGATTCTTTGTCAGGGGCCATCGAAGCTTTGAAAGCTAGCACACTTCGCCTTCCAGTTGTTGGTGTAGCAAGGGCTGATGTCTACAACGTGAAGGATGCTATTTCTTCAGCAGTTGATGTGATGCAAGCAATGGCATCATCAATATGCTTATTGTTATCGAAGGTTGGGGATGTGAACTTTTTGGTGGCTGAACTTGCAAATGTAACTGCGAAGGAGCATGCTTTGCTTGGTCAGTGCAGAGACCTATTGTCAACAGTTGTAGCCATGCAGGTGGAGGAATGTAGCCTCAGGACACATATTTTACAACGGAAACGCGTACCTGACAGCCTCACAACGGAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

644

Amino Acids

68.99

Weight (kDa)

10.54

Isoelectric Point (pI)

67.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 283 - 605 1.9e-108 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1846
AasI GACNNNNNNGTC 2 cut(s) 113, 265
AbsI CCTCGAGG 1 cut(s) 1083
Acc36I ACCTGC 5 cut(s) 16, 1041, 1147, 1152, 1846
Acc65I GGTACC 1 cut(s) 4
AccB1I GGYRCC 4 cut(s) 4, 71, 475, 1171
AccB7I CCANNNNNTGG 1 cut(s) 1646
AccBSI CCGCTC 1 cut(s) 539
AccI GTMKAC 2 cut(s) 17, 1668
AccII CGCG 3 cut(s) 41, 383, 1902
AclWI GGATC 5 cut(s) 353, 549, 664, 1493, 1581
AcsI RAATTY 3 cut(s) 202, 553, 727
AcuI CTGAAG 2 cut(s) 1245, 1677
AcyI GRCGYC 4 cut(s) 114, 260, 476, 527
AfaI GTAC 4 cut(s) 6, 161, 844, 1905
AfeI AGCGCT 1 cut(s) 284
AfiI CCNNNNNNNGG 7 cut(s) 68, 1124, 1190, 1207, 1212, 1646, 1921
AflIII ACRYGT 3 cut(s) 162, 1327, 1900
AgsI TTSAA 7 cut(s) 28, 201, 207, 558, 1455, 1516, 1618
AhdI GACNNNNNGTC 1 cut(s) 1834
AhlI ACTAGT 1 cut(s) 1448
AjnI CCWGG 2 cut(s) 453, 621
Alw26I GTCTC 3 cut(s) 486, 956, 1821
AlwI GGATC 5 cut(s) 353, 549, 664, 1493, 1581
AlwNI CAGNNNCTG 1 cut(s) 577
Ama87I CYCGRG 3 cut(s) 141, 1083, 1206
Aor51HI AGCGCT 1 cut(s) 284
AoxI GGCC 6 cut(s) 80, 581, 663, 917, 923, 1602
ApeKI GCWGC 7 cut(s) 243, 571, 574, 1047, 1130, 1277, 1400
ApoI RAATTY 3 cut(s) 202, 553, 727
Asp718I GGTACC 1 cut(s) 4
AspLEI GCGC 3 cut(s) 285, 478, 981
AspS9I GGNCC 5 cut(s) 663, 917, 940, 1410, 1602
AsuC2I CCSGG 2 cut(s) 1207, 1208
AsuHPI GGTGA 5 cut(s) 661, 1137, 1173, 1222, 1345
AsuII TTCGAA 1 cut(s) 1288
AsuNHI GCTAGC 2 cut(s) 362, 1622
AvaI CYCGRG 3 cut(s) 141, 1083, 1206
AvaII GGWCC 2 cut(s) 940, 1410
AxyI CCTNAGG 1 cut(s) 1874
BanI GGYRCC 4 cut(s) 4, 71, 475, 1171
BanII GRGCYC 1 cut(s) 1202
BauI CACGAG 1 cut(s) 860
BbsI GAAGAC 1 cut(s) 825
BbvCI CCTCAGC 1 cut(s) 1502
BbvI GCAGC 7 cut(s) 230, 583, 586, 1059, 1117, 1264, 1387
BccI CCATC 6 cut(s) 688, 704, 928, 1183, 1535, 1613
BciT130I CCWGG 2 cut(s) 455, 623
BcnI CCSGG 2 cut(s) 1207, 1208
BcoDI GTCTC 3 cut(s) 486, 956, 1821
BcuI ACTAGT 1 cut(s) 1448
BfaI CTAG 6 cut(s) 363, 927, 1380, 1397, 1449, 1623
BfoI RGCGCY 2 cut(s) 286, 479
BfuAI ACCTGC 5 cut(s) 16, 1041, 1147, 1152, 1846
BlpI GCTNAGC 1 cut(s) 1157
BmcAI AGTACT 1 cut(s) 844
Bme1390I CCNGG 4 cut(s) 455, 623, 1207, 1208
Bme18I GGWCC 2 cut(s) 940, 1410
BmeRI GACNNNNNGTC 1 cut(s) 1834
BmeT110I CYCGRG 3 cut(s) 141, 1083, 1206
BmgT120I GGNCC 5 cut(s) 663, 917, 940, 1410, 1602
BmiI GGNNCC 8 cut(s) 6, 73, 477, 620, 664, 942, 1173, 1603
BmrFI CCNGG 4 cut(s) 455, 623, 1207, 1208
BmsI GCATC 4 cut(s) 1312, 1672, 1698, 1730
BmtI GCTAGC 2 cut(s) 366, 1626
BpiI GAAGAC 1 cut(s) 825
BplI GAGNNNNNCTC 2 cut(s) 431, 463
BpmI CTGGAG 1 cut(s) 437
Bpu10I CCTNAGC 1 cut(s) 1502
Bpu1102I GCTNAGC 1 cut(s) 1157
Bpu14I TTCGAA 1 cut(s) 1288
BpuMI CCSGG 2 cut(s) 1207, 1208
BsaHI GRCGYC 4 cut(s) 114, 260, 476, 527
BsaI GGTCTC 1 cut(s) 1821
BsaJI CCNNGG 4 cut(s) 46, 381, 920, 1206
BsaWI WCCGGW 1 cut(s) 1311
BsaXI ACNNNNNCTCC 4 cut(s) 152, 182, 1212, 1242
Bsc4I CCNNNNNNNGG 7 cut(s) 68, 1124, 1190, 1207, 1212, 1646, 1921
Bse118I RCCGGY 2 cut(s) 472, 1350
Bse1I ACTGG 4 cut(s) 244, 764, 1070, 1640
Bse21I CCTNAGG 1 cut(s) 1874
Bse3DI GCAATG 1 cut(s) 1722
BseBI CCWGG 2 cut(s) 455, 623
BseDI CCNNGG 4 cut(s) 46, 381, 920, 1206
BseGI GGATG 6 cut(s) 640, 706, 939, 1527, 1687, 1762
BseLI CCNNNNNNNGG 7 cut(s) 68, 1124, 1190, 1207, 1212, 1646, 1921
BseMI GCAATG 1 cut(s) 1722
BseMII CTCAG 5 cut(s) 750, 789, 1171, 1493, 1888
BseNI ACTGG 4 cut(s) 244, 764, 1070, 1640
BseXI GCAGC 7 cut(s) 230, 583, 586, 1059, 1117, 1264, 1387
BsgI GTGCAG 2 cut(s) 1386, 1843
Bsh1236I CGCG 3 cut(s) 41, 383, 1902
BshFI GGCC 6 cut(s) 82, 583, 665, 919, 925, 1604
BshNI GGYRCC 4 cut(s) 4, 71, 475, 1171
BsiHKCI CYCGRG 3 cut(s) 141, 1083, 1206
BsiSI CCGG 5 cut(s) 473, 506, 1207, 1312, 1351
BslFI GGGAC 3 cut(s) 28, 926, 1551
BslI CCNNNNNNNGG 7 cut(s) 68, 1124, 1190, 1207, 1212, 1646, 1921
BsmAI GTCTC 3 cut(s) 486, 956, 1821
BsmBI CGTCTC 1 cut(s) 486
BsmFI GGGAC 3 cut(s) 28, 926, 1551
BsnI GGCC 6 cut(s) 82, 583, 665, 919, 925, 1604
Bso31I GGTCTC 1 cut(s) 1821
BsoBI CYCGRG 3 cut(s) 141, 1083, 1206
Bsp119I TTCGAA 1 cut(s) 1288
Bsp1286I GDGCHC 1 cut(s) 1202
Bsp143I GATC 5 cut(s) 345, 541, 656, 1485, 1573
Bsp1720I GCTNAGC 1 cut(s) 1157
BspANI GGCC 6 cut(s) 82, 583, 665, 919, 925, 1604
BspCNI CTCAG 5 cut(s) 751, 790, 1170, 1494, 1887
BspFNI CGCG 3 cut(s) 41, 383, 1902
BspLI GGNNCC 8 cut(s) 6, 73, 477, 620, 664, 942, 1173, 1603
BspMI ACCTGC 5 cut(s) 16, 1041, 1147, 1152, 1846
BspOI GCTAGC 2 cut(s) 366, 1626
BspPI GGATC 5 cut(s) 353, 549, 664, 1493, 1581
BspT104I TTCGAA 1 cut(s) 1288
BspT107I GGYRCC 4 cut(s) 4, 71, 475, 1171
BspTNI GGTCTC 1 cut(s) 1821
BsrBI CCGCTC 1 cut(s) 539
BsrDI GCAATG 1 cut(s) 1722
BsrFI RCCGGY 2 cut(s) 472, 1350
BsrI ACTGG 4 cut(s) 244, 764, 1070, 1640
BssAI RCCGGY 2 cut(s) 472, 1350
BssECI CCNNGG 4 cut(s) 46, 381, 920, 1206
BssMI GATC 5 cut(s) 345, 541, 656, 1485, 1573
BssNI GRCGYC 4 cut(s) 114, 260, 476, 527
BssSI CACGAG 1 cut(s) 860
BssT1I CCWWGG 1 cut(s) 46
Bst2BI CACGAG 1 cut(s) 860
Bst2UI CCWGG 2 cut(s) 455, 623
Bst4CI ACNGT 4 cut(s) 76, 399, 842, 1843
Bst6I CTCTTC 5 cut(s) 93, 184, 638, 1285, 1551
BstACI GRCGYC 4 cut(s) 114, 260, 476, 527
BstAPI GCANNNNNTGC 1 cut(s) 1810
BstBI TTCGAA 1 cut(s) 1288
BstC8I GCNNGC 8 cut(s) 287, 364, 474, 503, 585, 1624, 1713, 1806
BstDEI CTNAG 5 cut(s) 759, 798, 1157, 1502, 1874
BstDSI CCRYGG 2 cut(s) 381, 920
BstF5I GGATG 6 cut(s) 640, 706, 939, 1527, 1687, 1762
BstFNI CGCG 3 cut(s) 41, 383, 1902
BstH2I RGCGCY 2 cut(s) 286, 479
BstHHI GCGC 3 cut(s) 285, 478, 981
BstKTI GATC 5 cut(s) 348, 544, 659, 1488, 1576
BstMAI GTCTC 3 cut(s) 486, 956, 1821
BstMBI GATC 5 cut(s) 345, 541, 656, 1485, 1573
BstNI CCWGG 2 cut(s) 455, 623
BstNSI RCATGY 5 cut(s) 166, 379, 1258, 1331, 1808
BstSCI CCNGG 4 cut(s) 453, 621, 1205, 1206
BstUI CGCG 3 cut(s) 41, 383, 1902
BstV1I GCAGC 7 cut(s) 230, 583, 586, 1059, 1117, 1264, 1387
BstV2I GAAGAC 1 cut(s) 825
BstX2I RGATCY 1 cut(s) 656
BstXI CCANNNNNNTGG 1 cut(s) 1858
BstYI RGATCY 1 cut(s) 656
Bsu36I CCTNAGG 1 cut(s) 1874
BsuRI GGCC 6 cut(s) 82, 583, 665, 919, 925, 1604
BtgI CCRYGG 2 cut(s) 381, 920
BtsCI GGATG 6 cut(s) 640, 706, 939, 1527, 1687, 1762
BtsI GCAGTG 1 cut(s) 1367
BtsIMutI CAGTG 4 cut(s) 753, 1077, 1367, 1826
BveI ACCTGC 5 cut(s) 16, 1041, 1147, 1152, 1846
Cac8I GCNNGC 8 cut(s) 287, 364, 474, 503, 585, 1624, 1713, 1806
CaiI CAGNNNCTG 1 cut(s) 577
CfoI GCGC 3 cut(s) 285, 478, 981
Cfr10I RCCGGY 2 cut(s) 472, 1350
Cfr13I GGNCC 5 cut(s) 663, 917, 940, 1410, 1602
Cfr42I CCGCGG 1 cut(s) 384
Cfr9I CCCGGG 1 cut(s) 1206
CseI GACGC 4 cut(s) 30, 103, 268, 535
Csp6I GTAC 4 cut(s) 5, 160, 843, 1904
CviAII CATG 7 cut(s) 163, 376, 1255, 1328, 1440, 1805, 1852
CviQI GTAC 4 cut(s) 5, 160, 843, 1904
DdeI CTNAG 5 cut(s) 759, 798, 1157, 1502, 1874
DinI GGCGCC 1 cut(s) 477
DpnI GATC 5 cut(s) 347, 543, 658, 1487, 1575
DpnII GATC 5 cut(s) 345, 541, 656, 1485, 1573
DrdI GACNNNNNNGTC 2 cut(s) 113, 265
DriI GACNNNNNGTC 1 cut(s) 1834
DseDI GACNNNNNNGTC 2 cut(s) 113, 265
Eam1104I CTCTTC 5 cut(s) 93, 184, 638, 1285, 1551
Eam1105I GACNNNNNGTC 1 cut(s) 1834
EarI CTCTTC 5 cut(s) 93, 184, 638, 1285, 1551
Eco130I CCWWGG 1 cut(s) 46
Eco24I GRGCYC 1 cut(s) 1202
Eco31I GGTCTC 1 cut(s) 1821
Eco47I GGWCC 2 cut(s) 940, 1410
Eco47III AGCGCT 1 cut(s) 284
Eco57I CTGAAG 2 cut(s) 1245, 1677
Eco81I CCTNAGG 1 cut(s) 1874
Eco88I CYCGRG 3 cut(s) 141, 1083, 1206
EcoRI GAATTC 2 cut(s) 553, 727
EcoRII CCWGG 2 cut(s) 453, 621
EcoT14I CCWWGG 1 cut(s) 46
EcoT22I ATGCAT 1 cut(s) 1441
EcoT38I GRGCYC 1 cut(s) 1202
EgeI GGCGCC 1 cut(s) 477
EheI GGCGCC 1 cut(s) 477
ErhI CCWWGG 1 cut(s) 46
Esp3I CGTCTC 1 cut(s) 486
FaeI CATG 7 cut(s) 166, 379, 1258, 1331, 1443, 1808, 1855
FaqI GGGAC 3 cut(s) 28, 926, 1551
FatI CATG 7 cut(s) 162, 375, 1254, 1327, 1439, 1804, 1851
FblI GTMKAC 2 cut(s) 17, 1668
FokI GGATG 6 cut(s) 647, 713, 946, 1514, 1694, 1769
FriOI GRGCYC 1 cut(s) 1202
FspBI CTAG 6 cut(s) 363, 927, 1380, 1397, 1449, 1623
GlaI GCGC 3 cut(s) 284, 477, 980
GsuI CTGGAG 1 cut(s) 437
HaeII RGCGCY 2 cut(s) 286, 479
HaeIII GGCC 6 cut(s) 82, 583, 665, 919, 925, 1604
HapII CCGG 5 cut(s) 473, 506, 1207, 1312, 1351
HgaI GACGC 4 cut(s) 30, 103, 268, 535
HhaI GCGC 3 cut(s) 285, 478, 981
Hin1I GRCGYC 4 cut(s) 114, 260, 476, 527
Hin1II CATG 7 cut(s) 166, 379, 1258, 1331, 1443, 1808, 1855
Hin6I GCGC 3 cut(s) 283, 476, 979
HinP1I GCGC 3 cut(s) 283, 476, 979
HincII GTYRAC 2 cut(s) 18, 1839
HindII GTYRAC 2 cut(s) 18, 1839
HindIII AAGCTT 1 cut(s) 1611
HinfI GANTC 6 cut(s) 101, 440, 818, 992, 1466, 1589
HpaII CCGG 5 cut(s) 473, 506, 1207, 1312, 1351
HphI GGTGA 5 cut(s) 661, 1137, 1173, 1222, 1345
Hpy166II GTNNAC 5 cut(s) 18, 402, 1669, 1762, 1839
Hpy8I GTNNAC 5 cut(s) 18, 402, 1669, 1762, 1839
Hpy99I CGWCG 7 cut(s) 22, 119, 223, 253, 262, 412, 529
HpyAV CCTTC 8 cut(s) 105, 511, 1212, 1269, 1646, 1672, 1741, 1792
HpyCH4III ACNGT 4 cut(s) 76, 399, 842, 1843
HpyCH4IV ACGT 1 cut(s) 1674
HpyF3I CTNAG 5 cut(s) 759, 798, 1157, 1502, 1874
HpySE526I ACGT 1 cut(s) 1674
Hsp92I GRCGYC 4 cut(s) 114, 260, 476, 527
Hsp92II CATG 7 cut(s) 166, 379, 1258, 1331, 1443, 1808, 1855
HspAI GCGC 3 cut(s) 283, 476, 979
KasI GGCGCC 1 cut(s) 475
KpnI GGTACC 1 cut(s) 8
KroI GCCGGC 1 cut(s) 472
KroNI GCCGGC 1 cut(s) 474
KspI CCGCGG 1 cut(s) 384
Kzo9I GATC 5 cut(s) 345, 541, 656, 1485, 1573
LmnI GCTCC 4 cut(s) 414, 618, 1127, 1801
Lsp1109I GCAGC 7 cut(s) 230, 583, 586, 1059, 1117, 1264, 1387
LweI GCATC 4 cut(s) 1312, 1672, 1698, 1730
MaeI CTAG 6 cut(s) 363, 927, 1380, 1397, 1449, 1623
MaeII ACGT 1 cut(s) 1674
MaeIII GTNAC 8 cut(s) 148, 313, 520, 1228, 1444, 1491, 1563, 1789
MalI GATC 5 cut(s) 347, 543, 658, 1487, 1575
MbiI CCGCTC 1 cut(s) 539
MboI GATC 5 cut(s) 345, 541, 656, 1485, 1573
MflI RGATCY 1 cut(s) 656
MhlI GDGCHC 1 cut(s) 1202
MluCI AATT 7 cut(s) 202, 328, 553, 727, 736, 778, 847
MluI ACGCGT 1 cut(s) 1900
Mly113I GGCGCC 1 cut(s) 476
MlyI GAGTC 2 cut(s) 449, 1001
MmeI TCCRAC 4 cut(s) 129, 861, 889, 1282
Mph1103I ATGCAT 1 cut(s) 1441
MroNI GCCGGC 1 cut(s) 472
MseI TTAA 1 cut(s) 1268
MslI CAYNNNNRTG 2 cut(s) 1729, 1856
MspA1I CMGCKG 2 cut(s) 383, 577
MspI CCGG 5 cut(s) 473, 506, 1207, 1312, 1351
MspR9I CCNGG 4 cut(s) 455, 623, 1207, 1208
MvaI CCWGG 2 cut(s) 455, 623
MvnI CGCG 3 cut(s) 41, 383, 1902
NaeI GCCGGC 1 cut(s) 474
NarI GGCGCC 1 cut(s) 476
NciI CCSGG 2 cut(s) 1207, 1208
NdeII GATC 5 cut(s) 345, 541, 656, 1485, 1573
NgoMIV GCCGGC 1 cut(s) 472
NheI GCTAGC 2 cut(s) 362, 1622
NlaIII CATG 7 cut(s) 166, 379, 1258, 1331, 1443, 1808, 1855
NlaIV GGNNCC 8 cut(s) 6, 73, 477, 620, 664, 942, 1173, 1603
NmuCI GTSAC 4 cut(s) 148, 313, 520, 1563
NsiI ATGCAT 1 cut(s) 1441
NspI RCATGY 5 cut(s) 166, 379, 1258, 1331, 1808
NspV TTCGAA 1 cut(s) 1288
PaeI GCATGC 1 cut(s) 1808
PaeR7I CTCGAG 1 cut(s) 1083
PaqCI CACCTGC 1 cut(s) 1846
PciI ACATGT 2 cut(s) 162, 1327
PcsI WCGNNNNNNNCGW 2 cut(s) 227, 254
PdiI GCCGGC 1 cut(s) 474
PfeI GAWTC 4 cut(s) 101, 818, 1466, 1589
PflMI CCANNNNNTGG 1 cut(s) 1646
PleI GAGTC 2 cut(s) 448, 1000
PluTI GGCGCC 1 cut(s) 479
PpsI GAGTC 2 cut(s) 448, 1000
PscI ACATGT 2 cut(s) 162, 1327
Psp6I CCWGG 2 cut(s) 453, 621
PspGI CCWGG 2 cut(s) 453, 621
PspN4I GGNNCC 8 cut(s) 6, 73, 477, 620, 664, 942, 1173, 1603
PspPI GGNCC 5 cut(s) 663, 917, 940, 1410, 1602
PspXI VCTCGAGB 1 cut(s) 1083
PstNI CAGNNNCTG 1 cut(s) 577
PsuI RGATCY 1 cut(s) 656
PvuII CAGCTG 1 cut(s) 577
RsaI GTAC 4 cut(s) 6, 161, 844, 1905
RsaNI GTAC 4 cut(s) 5, 160, 843, 1904
RseI CAYNNNNRTG 2 cut(s) 1729, 1856
SacII CCGCGG 1 cut(s) 384
SalI GTCGAC 1 cut(s) 16
SaqAI TTAA 1 cut(s) 1268
Sau3AI GATC 5 cut(s) 345, 541, 656, 1485, 1573
Sau96I GGNCC 5 cut(s) 663, 917, 940, 1410, 1602
ScaI AGTACT 1 cut(s) 844
SchI GAGTC 2 cut(s) 449, 1001
ScrFI CCNGG 4 cut(s) 455, 623, 1207, 1208
SduI GDGCHC 1 cut(s) 1202
SfaNI GCATC 4 cut(s) 1312, 1672, 1698, 1730
SfoI GGCGCC 1 cut(s) 477
Sfr274I CTCGAG 1 cut(s) 1083
Sfr303I CCGCGG 1 cut(s) 384
SfuI TTCGAA 1 cut(s) 1288
SgrBI CCGCGG 1 cut(s) 384
SinI GGWCC 2 cut(s) 940, 1410
SlaI CTCGAG 1 cut(s) 1083
SmaI CCCGGG 1 cut(s) 1208
SmiMI CAYNNNNRTG 2 cut(s) 1729, 1856
SmlI CTYRAG 1 cut(s) 1083
SmoI CTYRAG 1 cut(s) 1083
SpeI ACTAGT 1 cut(s) 1448
SphI GCATGC 1 cut(s) 1808
Sse9I AATT 7 cut(s) 202, 328, 553, 727, 736, 778, 847
SspDI GGCGCC 1 cut(s) 475
SspMI CTAG 6 cut(s) 363, 927, 1380, 1397, 1449, 1623
StyD4I CCNGG 4 cut(s) 453, 621, 1205, 1206
StyI CCWWGG 1 cut(s) 46
TaaI ACNGT 4 cut(s) 76, 399, 842, 1843
TaiI ACGT 1 cut(s) 1677
TasI AATT 7 cut(s) 202, 328, 553, 727, 736, 778, 847
TatI WGTACW 1 cut(s) 842
TfiI GAWTC 4 cut(s) 101, 818, 1466, 1589
Tru1I TTAA 1 cut(s) 1268
Tru9I TTAA 1 cut(s) 1268
TscAI CASTG 4 cut(s) 753, 1077, 1367, 1826
TseFI GTSAC 4 cut(s) 148, 313, 520, 1563
TseI GCWGC 7 cut(s) 243, 571, 574, 1047, 1130, 1277, 1400
Tsp45I GTSAC 4 cut(s) 148, 313, 520, 1563
TspGWI ACGGA 4 cut(s) 207, 647, 1498, 1909
TspMI CCCGGG 1 cut(s) 1206
TspRI CASTG 4 cut(s) 753, 1077, 1367, 1826
Van91I CCANNNNNTGG 1 cut(s) 1646
VpaK11BI GGWCC 2 cut(s) 940, 1410
XapI RAATTY 3 cut(s) 202, 553, 727
XceI RCATGY 5 cut(s) 166, 379, 1258, 1331, 1808
XhoI CTCGAG 1 cut(s) 1083
XmaI CCCGGG 1 cut(s) 1206
XmiI GTMKAC 2 cut(s) 17, 1668
XspI CTAG 6 cut(s) 363, 927, 1380, 1397, 1449, 1623
ZrmI AGTACT 1 cut(s) 844
Zsp2I ATGCAT 1 cut(s) 1441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.