FvH4_2g33270

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001505
Physical Location & Seq
Reverse (-)
24999737 .. 25007965
8229 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33270.t1

Sequence Viewer

Length: 1890 bp
ATGGTAGCTGCTGTATCGACGACATTGAATCCCAAAGCCGCCGGTCGTCCTCAGAACGGAACCAGGCCTCCTTTATTACCCTCCGACCCCGAAAATGGCGTCGCTCATCGCCGTCCCAAGGCTCGAGAGGTCACCTCCCGCTACATGTCGTCTTCTTCCTCCACGGCTTCGTCTAATTGTTCCAATTCGAAGCGATGTCCGTCGCCGGTGGTGTCCAGAACCGCCGCGGCGAGTCCGATGCCGACGCCGCAGACGTTGAGGCGGTCGCAGTCGGTGGAGAGGAGGAGAGCGGCGACGCCTCGGCCGAACTCGTTGGATTTGAGGACCGGGAACGGAGCTGCCGCCGGCGGTGAAATGTCGGCGGCTCAGAAGCTGCTGGTTACGTCGACGAGGAGTTTATCGGTTTCGTTTCAGGGGGAGTCGTACTCGCTTCAGGTGAGTAAAGCGAAGCGAGCGCCGTCTCCGACTGCGAGGAAAGGCACGCCGGAGAGGAGGAAGGTTTCGACGCCGGCGAGGCCGGATCAGAGTGAGAATTCGAGGCCGATGGATCAGTACCGGTGGCCGGCGAGGTTGAGGCCGGACACGTGTATGACGAAGAGCTTGGATTGTACGGTGGAGAGGAAGAAGGTGAATGGATCTGGCTCCAATGTGGTTAGGGCATTGCAAAACTCGCTGGGGAATGATCTTGATGGGAGGTTGAGGTCAATTTCGTGTAATTTGGGTTCTGTGAAAGCTACTGAGTCTGTTGATGAAGCTAAGTCGGTGAGAGTTACACAGTCTGAAGCTGTGGCATCGTCTGATACCGATAGTGTGTCTTCTGGTAGTTCCAATTCGGGGCCTCATGAGCTCGGTGGAGGTGTCTCTGGTGCTGTTGGACAAGCGCCACGGCCTAGAGGGATAGTGGTTCCGGCCAGGTTTATGCAAGAGACTAACAATCTCTTGCGCAGGCAGTCTGAGTATAAGGCTAATGGAGCAAGAATTATAGCATCTCCTAAGGTTAATGAGTTGAATAGGTTATCTGTTGATAGTCCTGTGTCATCTCCTCGAGGTGTTGTGAATAGCAGAGGCCAGTTATCCCCAATTCGTGGATCTGCTAGGCCTGCATCGCCAAGTAAGCTCAGCAGGCCATCTGTATCGTCCCCTTCGAGGGGAGTGAGCCCTTCTCGAGTAAGGAATGGAGTGTCAGCTACACCAAGTAGTAATTTGAGCAACATTCCTTCGGTTTTAAGTTTTGCTGCTGATGTTCGAAGAGGGAAGACTGGGGAGGAACAGATAGAGTATGCACATGTGTTGAGGCTCTTACATAACCGGCAGTTGCAGTTGCGTTTCGTAAATGCTAGAGCTAATGCTGCACTAGCTGCACAGAGGTCCAATGCAGAGAGAAGTTTATACAATGCATGGATAACAACTTCAAAACTCCGGGAATCTGTTAGAGCCAAAAGAACTGAGTTACAACTACTGAGGCAAAATTTGAAGTTAACTTCCATCCTCCAGGGCCAAATGACATATTTGGAAGAGTGTTCCCTTCTGGATCGGGATTACTCTAATTCCTTGTCCGGTGCCATTGAAGCTTTGAGGGCTAGCACACTCCGCCTTCCAGTGGTTGATGGAGCAAGGGCAGATGTCCACAATGTGAAGGACGCTATTTCTTCAGCAGTTGATGTGATGCAGGCAATGGCATCATCAATATGCTTATTATTATCTAAGGTTGGGGAGGTGAACTCTTTGGCGGATGAACTTGCAAACGTAAGTGTCAAGGAGCATGCTTTGCTTGGTCAATGCAGAGATCTGTTGTCAACAGTTGCAGCCATGCAGGTTGAGGAATGTAGCCTGAGAACACATATTCTACAACAGGAACGTGTACCTTCAAGCCTCATAGCAGAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

630

Amino Acids

67.4

Weight (kDa)

10.49

Isoelectric Point (pI)

63.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 270 - 591 1.9e-106 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1044
Acc16I TGCGCA 1 cut(s) 944
Acc36I ACCTGC 1 cut(s) 1804
AccB1I GGYRCC 1 cut(s) 1559
AccB7I CCANNNNNTGG 1 cut(s) 1085
AccBSI CCGCTC 1 cut(s) 290
AccI GTMKAC 1 cut(s) 386
AccII CGCG 1 cut(s) 227
AclWI GGATC 5 cut(s) 528, 555, 643, 1096, 1539
AcoI YGGCCR 3 cut(s) 302, 560, 909
AcsI RAATTY 2 cut(s) 532, 1468
AcuI CTGAAG 3 cut(s) 416, 801, 1635
AcvI CACGTG 1 cut(s) 585
AcyI GRCGYC 4 cut(s) 99, 245, 296, 506
AdeI CACNNNGTG 1 cut(s) 1633
AfaI GTAC 4 cut(s) 425, 554, 610, 1863
AflIII ACRYGT 5 cut(s) 144, 582, 584, 1285, 1858
AgeI ACCGGT 1 cut(s) 555
AgsI TTSAA 6 cut(s) 28, 1009, 1413, 1474, 1568, 1869
AjnI CCWGG 3 cut(s) 62, 911, 1491
AloI GAACNNNNNNTCC 2 cut(s) 52, 84
Alw21I GWGCWC 1 cut(s) 849
Alw26I GTCTC 3 cut(s) 465, 865, 920
AlwI GGATC 5 cut(s) 528, 555, 643, 1096, 1539
AlwNI CAGNNNCTG 1 cut(s) 373
Ama87I CYCGRG 3 cut(s) 123, 1044, 1164
ApeKI GCWGC 7 cut(s) 8, 338, 373, 1235, 1349, 1358, 1805
ApoI RAATTY 2 cut(s) 532, 1468
ArsI GACNNNNNNTTYG 2 cut(s) 84, 116
AsiGI ACCGGT 1 cut(s) 555
AspLEI GCGC 3 cut(s) 457, 883, 945
AspS9I GGNCC 4 cut(s) 324, 836, 1368, 1495
AsuC2I CCSGG 2 cut(s) 328, 1421
AsuHPI GGTGA 6 cut(s) 124, 362, 448, 640, 775, 1729
AsuII TTCGAA 2 cut(s) 188, 1246
AsuNHI GCTAGC 1 cut(s) 1580
AvaI CYCGRG 3 cut(s) 123, 1044, 1164
AvaII GGWCC 2 cut(s) 324, 1368
AxyI CCTNAGG 1 cut(s) 993
BanI GGYRCC 1 cut(s) 1559
BanII GRGCYC 2 cut(s) 849, 1160
BbrPI CACGTG 1 cut(s) 585
BbsI GAAGAC 3 cut(s) 144, 807, 1262
Bbv12I GWGCWC 1 cut(s) 849
BbvI GCAGC 6 cut(s) 325, 360, 1222, 1336, 1345, 1817
BccI CCATC 5 cut(s) 538, 683, 1135, 1493, 1601
BceAI ACGGC 4 cut(s) 96, 180, 442, 902
BcgI CGANNNNNNTGC 2 cut(s) 220, 254
BciT130I CCWGG 3 cut(s) 64, 913, 1493
BcnI CCSGG 2 cut(s) 328, 1421
BcoDI GTCTC 3 cut(s) 465, 865, 920
BfaI CTAG 5 cut(s) 891, 1095, 1338, 1355, 1581
BfoI RGCGCY 2 cut(s) 458, 884
BfuAI ACCTGC 1 cut(s) 1804
BglI GCCNNNNNGGC 1 cut(s) 514
BglII AGATCT 1 cut(s) 1786
BlpI GCTNAGC 1 cut(s) 1118
Bme1390I CCNGG 5 cut(s) 64, 328, 913, 1421, 1493
Bme18I GGWCC 2 cut(s) 324, 1368
BmeT110I CYCGRG 3 cut(s) 123, 1044, 1164
BmgT120I GGNCC 4 cut(s) 324, 836, 1368, 1495
BmiI GGNNCC 5 cut(s) 61, 643, 837, 906, 1561
BmrFI CCNGG 5 cut(s) 64, 328, 913, 1421, 1493
BmrI ACTGGG 1 cut(s) 1269
BmsI GCATC 6 cut(s) 228, 800, 995, 1112, 1656, 1688
BmtI GCTAGC 1 cut(s) 1584
BmuI ACTGGG 1 cut(s) 1269
BpiI GAAGAC 3 cut(s) 144, 807, 1262
BplI GAGNNNNNCTC 8 cut(s) 119, 151, 410, 442, 1147, 1179, 1706, 1738
BpmI CTGGAG 1 cut(s) 1475
Bpu1102I GCTNAGC 1 cut(s) 1118
Bpu14I TTCGAA 2 cut(s) 188, 1246
BpuMI CCSGG 2 cut(s) 328, 1421
BsaAI YACGTR 1 cut(s) 585
BsaHI GRCGYC 4 cut(s) 99, 245, 296, 506
BsaJI CCNNGG 6 cut(s) 117, 162, 225, 299, 884, 1492
BsaWI WCCGGW 2 cut(s) 555, 1556
BsaXI ACNNNNNCTCC 2 cut(s) 52, 82
Bse118I RCCGGY 7 cut(s) 41, 205, 344, 508, 555, 562, 1308
Bse1I ACTGG 3 cut(s) 1069, 1264, 1598
Bse21I CCTNAGG 1 cut(s) 993
Bse3DI GCAATG 2 cut(s) 659, 1680
BseBI CCWGG 3 cut(s) 64, 913, 1493
BseDI CCNNGG 6 cut(s) 117, 162, 225, 299, 884, 1492
BseGI GGATG 2 cut(s) 1485, 1738
BseMI GCAATG 2 cut(s) 659, 1680
BseMII CTCAG 8 cut(s) 65, 380, 729, 945, 1132, 1437, 1451, 1823
BseNI ACTGG 3 cut(s) 1069, 1264, 1598
BseRI GAGGAG 5 cut(s) 295, 298, 406, 505, 1032
BseX3I CGGCCG 1 cut(s) 302
BseXI GCAGC 6 cut(s) 325, 360, 1222, 1336, 1345, 1817
BseYI CCCAGC 1 cut(s) 673
BsgI GTGCAG 2 cut(s) 1335, 1344
Bsh1236I CGCG 1 cut(s) 227
Bsh1285I CGRYCG 3 cut(s) 46, 266, 305
BshNI GGYRCC 1 cut(s) 1559
BshTI ACCGGT 1 cut(s) 555
BsiEI CGRYCG 3 cut(s) 46, 266, 305
BsiHKAI GWGCWC 1 cut(s) 849
BsiHKCI CYCGRG 3 cut(s) 123, 1044, 1164
BslFI GGGAC 2 cut(s) 99, 1123
BsmAI GTCTC 3 cut(s) 465, 865, 920
BsmBI CGTCTC 1 cut(s) 465
BsmFI GGGAC 2 cut(s) 99, 1123
BsoBI CYCGRG 3 cut(s) 123, 1044, 1164
Bsp119I TTCGAA 2 cut(s) 188, 1246
Bsp1286I GDGCHC 2 cut(s) 849, 1160
Bsp143I GATC 7 cut(s) 520, 547, 635, 682, 1088, 1531, 1786
Bsp1720I GCTNAGC 1 cut(s) 1118
BspCNI CTCAG 8 cut(s) 64, 379, 730, 946, 1131, 1438, 1452, 1824
BspFNI CGCG 1 cut(s) 227
BspHI TCATGA 1 cut(s) 841
BspLI GGNNCC 5 cut(s) 61, 643, 837, 906, 1561
BspMI ACCTGC 1 cut(s) 1804
BspOI GCTAGC 1 cut(s) 1584
BspPI GGATC 5 cut(s) 528, 555, 643, 1096, 1539
BspQI GCTCTTC 1 cut(s) 590
BspT104I TTCGAA 2 cut(s) 188, 1246
BspT107I GGYRCC 1 cut(s) 1559
BsrBI CCGCTC 1 cut(s) 290
BsrDI GCAATG 2 cut(s) 659, 1680
BsrFI RCCGGY 7 cut(s) 41, 205, 344, 508, 555, 562, 1308
BsrI ACTGG 3 cut(s) 1069, 1264, 1598
BssAI RCCGGY 7 cut(s) 41, 205, 344, 508, 555, 562, 1308
BssECI CCNNGG 6 cut(s) 117, 162, 225, 299, 884, 1492
BssMI GATC 7 cut(s) 520, 547, 635, 682, 1088, 1531, 1786
BssNI GRCGYC 4 cut(s) 99, 245, 296, 506
BssT1I CCWWGG 1 cut(s) 117
Bst2UI CCWGG 3 cut(s) 64, 913, 1493
Bst4CI ACNGT 3 cut(s) 613, 777, 1801
Bst6I CTCTTC 3 cut(s) 590, 1243, 1509
BstACI GRCGYC 4 cut(s) 99, 245, 296, 506
BstAPI GCANNNNNTGC 2 cut(s) 1358, 1768
BstBAI YACGTR 1 cut(s) 585
BstBI TTCGAA 2 cut(s) 188, 1246
BstDSI CCRYGG 3 cut(s) 162, 225, 884
BstEII GGTNACC 1 cut(s) 130
BstF5I GGATG 2 cut(s) 1485, 1738
BstFNI CGCG 1 cut(s) 227
BstH2I RGCGCY 2 cut(s) 458, 884
BstHHI GCGC 3 cut(s) 457, 883, 945
BstKTI GATC 7 cut(s) 523, 550, 638, 685, 1091, 1534, 1789
BstMAI GTCTC 3 cut(s) 465, 865, 920
BstMBI GATC 7 cut(s) 520, 547, 635, 682, 1088, 1531, 1786
BstMCI CGRYCG 3 cut(s) 46, 266, 305
BstNI CCWGG 3 cut(s) 64, 913, 1493
BstNSI RCATGY 3 cut(s) 148, 1289, 1766
BstPI GGTNACC 1 cut(s) 130
BstSCI CCNGG 5 cut(s) 62, 326, 911, 1419, 1491
BstUI CGCG 1 cut(s) 227
BstV1I GCAGC 6 cut(s) 325, 360, 1222, 1336, 1345, 1817
BstV2I GAAGAC 3 cut(s) 144, 807, 1262
BstX2I RGATCY 3 cut(s) 635, 1088, 1786
BstYI RGATCY 3 cut(s) 635, 1088, 1786
BstZI CGGCCG 1 cut(s) 302
Bsu36I CCTNAGG 1 cut(s) 993
BtgI CCRYGG 3 cut(s) 162, 225, 884
BtgZI GCGATG 3 cut(s) 92, 208, 1089
BtsCI GGATG 2 cut(s) 1485, 1738
BtsIMutI CAGTG 1 cut(s) 1605
BveI ACCTGC 1 cut(s) 1804
CaiI CAGNNNCTG 1 cut(s) 373
CciI TCATGA 1 cut(s) 841
CfoI GCGC 3 cut(s) 457, 883, 945
Cfr10I RCCGGY 7 cut(s) 41, 205, 344, 508, 555, 562, 1308
Cfr13I GGNCC 4 cut(s) 324, 836, 1368, 1495
Cfr42I CCGCGG 1 cut(s) 228
CseI GACGC 5 cut(s) 88, 253, 304, 514, 1649
Csp6I GTAC 4 cut(s) 424, 553, 609, 1862
CspAI ACCGGT 1 cut(s) 555
CviAII CATG 6 cut(s) 145, 842, 1286, 1398, 1763, 1810
CviQI GTAC 4 cut(s) 424, 553, 609, 1862
DpnI GATC 7 cut(s) 522, 549, 637, 684, 1090, 1533, 1788
DpnII GATC 7 cut(s) 520, 547, 635, 682, 1088, 1531, 1786
DraIII CACNNNGTG 1 cut(s) 1633
EaeI YGGCCR 3 cut(s) 302, 560, 909
EagI CGGCCG 1 cut(s) 302
Eam1104I CTCTTC 3 cut(s) 590, 1243, 1509
EarI CTCTTC 3 cut(s) 590, 1243, 1509
EciI GGCGGA 2 cut(s) 1580, 1745
Ecl136II GAGCTC 1 cut(s) 847
EclXI CGGCCG 1 cut(s) 302
Eco130I CCWWGG 1 cut(s) 117
Eco147I AGGCCT 2 cut(s) 67, 1099
Eco24I GRGCYC 2 cut(s) 849, 1160
Eco47I GGWCC 2 cut(s) 324, 1368
Eco52I CGGCCG 1 cut(s) 302
Eco53kI GAGCTC 1 cut(s) 847
Eco57I CTGAAG 3 cut(s) 416, 801, 1635
Eco72I CACGTG 1 cut(s) 585
Eco81I CCTNAGG 1 cut(s) 993
Eco88I CYCGRG 3 cut(s) 123, 1044, 1164
Eco91I GGTNACC 1 cut(s) 130
EcoICRI GAGCTC 1 cut(s) 847
EcoO109I RGGNCCY 1 cut(s) 836
EcoO65I GGTNACC 1 cut(s) 130
EcoRI GAATTC 1 cut(s) 532
EcoRII CCWGG 3 cut(s) 62, 911, 1491
EcoT14I CCWWGG 1 cut(s) 117
EcoT22I ATGCAT 1 cut(s) 1399
EcoT38I GRGCYC 2 cut(s) 849, 1160
ErhI CCWWGG 1 cut(s) 117
Esp3I CGTCTC 1 cut(s) 465
FaeI CATG 6 cut(s) 148, 845, 1289, 1401, 1766, 1813
FaqI GGGAC 2 cut(s) 99, 1123
FatI CATG 6 cut(s) 144, 841, 1285, 1397, 1762, 1809
FauI CCCGC 1 cut(s) 146
FblI GTMKAC 1 cut(s) 386
FokI GGATG 2 cut(s) 1472, 1745
FriOI GRGCYC 2 cut(s) 849, 1160
FspBI CTAG 5 cut(s) 891, 1095, 1338, 1355, 1581
FspI TGCGCA 1 cut(s) 944
GlaI GCGC 3 cut(s) 456, 882, 944
GsaI CCCAGC 1 cut(s) 677
GsuI CTGGAG 1 cut(s) 1475
HaeII RGCGCY 2 cut(s) 458, 884
HgaI GACGC 5 cut(s) 88, 253, 304, 514, 1649
HhaI GCGC 3 cut(s) 457, 883, 945
Hin1I GRCGYC 4 cut(s) 99, 245, 296, 506
Hin1II CATG 6 cut(s) 148, 845, 1289, 1401, 1766, 1813
Hin6I GCGC 3 cut(s) 455, 881, 943
HinP1I GCGC 3 cut(s) 455, 881, 943
HincII GTYRAC 3 cut(s) 387, 1479, 1797
HindII GTYRAC 3 cut(s) 387, 1479, 1797
HindIII AAGCTT 1 cut(s) 1569
HinfI GANTC 5 cut(s) 28, 232, 419, 740, 1424
HpaI GTTAAC 1 cut(s) 1479
HphI GGTGA 6 cut(s) 124, 362, 448, 640, 775, 1729
Hpy166II GTNNAC 6 cut(s) 387, 1479, 1627, 1720, 1797, 1862
Hpy188I TCNGA 9 cut(s) 54, 85, 237, 369, 465, 525, 781, 799, 955
Hpy188III TCNNGA 7 cut(s) 125, 216, 686, 842, 1164, 1529, 1535
Hpy8I GTNNAC 6 cut(s) 387, 1479, 1627, 1720, 1797, 1862
Hpy99I CGWCG 8 cut(s) 22, 104, 205, 247, 298, 388, 391, 508
HpyAV CCTTC 9 cut(s) 490, 619, 1152, 1170, 1227, 1535, 1604, 1630, 1875
HpyCH4III ACNGT 3 cut(s) 613, 777, 1801
HpyCH4IV ACGT 5 cut(s) 254, 383, 584, 1746, 1858
HpySE526I ACGT 5 cut(s) 254, 383, 584, 1746, 1858
Hsp92I GRCGYC 4 cut(s) 99, 245, 296, 506
Hsp92II CATG 6 cut(s) 148, 845, 1289, 1401, 1766, 1813
HspAI GCGC 3 cut(s) 455, 881, 943
KroI GCCGGC 3 cut(s) 344, 508, 562
KroNI GCCGGC 3 cut(s) 346, 510, 564
KspAI GTTAAC 1 cut(s) 1479
KspI CCGCGG 1 cut(s) 228
Kzo9I GATC 7 cut(s) 520, 547, 635, 682, 1088, 1531, 1786
LguI GCTCTTC 1 cut(s) 590
LmnI GCTCC 5 cut(s) 335, 647, 971, 1610, 1759
Lsp1109I GCAGC 6 cut(s) 325, 360, 1222, 1336, 1345, 1817
LweI GCATC 6 cut(s) 228, 800, 995, 1112, 1656, 1688
MaeI CTAG 5 cut(s) 891, 1095, 1338, 1355, 1581
MaeII ACGT 5 cut(s) 254, 383, 584, 1746, 1858
MaeIII GTNAC 4 cut(s) 130, 379, 769, 1449
MalI GATC 7 cut(s) 522, 549, 637, 684, 1090, 1533, 1788
MbiI CCGCTC 1 cut(s) 290
MboI GATC 7 cut(s) 520, 547, 635, 682, 1088, 1531, 1786
MboII GAAGA 9 cut(s) 144, 147, 607, 634, 807, 1260, 1267, 1526, 1641
MflI RGATCY 3 cut(s) 635, 1088, 1786
MhlI GDGCHC 2 cut(s) 849, 1160
MlyI GAGTC 3 cut(s) 241, 428, 749
MmeI TCCRAC 4 cut(s) 108, 294, 488, 853
Mph1103I ATGCAT 1 cut(s) 1399
MreI CGCCGGCG 2 cut(s) 344, 508
MroNI GCCGGC 3 cut(s) 344, 508, 562
MseI TTAA 3 cut(s) 999, 1226, 1478
MslI CAYNNNNRTG 2 cut(s) 587, 1687
MspA1I CMGCKG 1 cut(s) 227
MspR9I CCNGG 5 cut(s) 64, 328, 913, 1421, 1493
MvaI CCWGG 3 cut(s) 64, 913, 1493
MvnI CGCG 1 cut(s) 227
NaeI GCCGGC 3 cut(s) 346, 510, 564
NciI CCSGG 2 cut(s) 328, 1421
NdeII GATC 7 cut(s) 520, 547, 635, 682, 1088, 1531, 1786
NgoMIV GCCGGC 3 cut(s) 344, 508, 562
NheI GCTAGC 1 cut(s) 1580
NlaIII CATG 6 cut(s) 148, 845, 1289, 1401, 1766, 1813
NlaIV GGNNCC 5 cut(s) 61, 643, 837, 906, 1561
NmeAIII GCCGAG 1 cut(s) 280
NmuCI GTSAC 1 cut(s) 130
NsbI TGCGCA 1 cut(s) 944
NsiI ATGCAT 1 cut(s) 1399
NspI RCATGY 3 cut(s) 148, 1289, 1766
NspV TTCGAA 2 cut(s) 188, 1246
PaeI GCATGC 1 cut(s) 1766
PaeR7I CTCGAG 3 cut(s) 123, 1044, 1164
PagI TCATGA 1 cut(s) 841
PceI AGGCCT 2 cut(s) 67, 1099
PciI ACATGT 2 cut(s) 144, 1285
PciSI GCTCTTC 1 cut(s) 590
PcsI WCGNNNNNNNCGW 5 cut(s) 251, 302, 509, 590, 1142
PdiI GCCGGC 3 cut(s) 346, 510, 564
PfeI GAWTC 2 cut(s) 28, 1424
PflMI CCANNNNNTGG 1 cut(s) 1085
PfoI TCCNGGA 1 cut(s) 1419
PinAI ACCGGT 1 cut(s) 555
PleI GAGTC 3 cut(s) 240, 427, 748
PmaCI CACGTG 1 cut(s) 585
PmlI CACGTG 1 cut(s) 585
PpsI GAGTC 3 cut(s) 240, 427, 748
Ppu21I YACGTR 1 cut(s) 585
PscI ACATGT 2 cut(s) 144, 1285
Psp124BI GAGCTC 1 cut(s) 849
Psp6I CCWGG 3 cut(s) 62, 911, 1491
PspCI CACGTG 1 cut(s) 585
PspEI GGTNACC 1 cut(s) 130
PspFI CCCAGC 1 cut(s) 673
PspGI CCWGG 3 cut(s) 62, 911, 1491
PspN4I GGNNCC 5 cut(s) 61, 643, 837, 906, 1561
PspPI GGNCC 4 cut(s) 324, 836, 1368, 1495
PspXI VCTCGAGB 1 cut(s) 1044
PsrI GAACNNNNNNTAC 2 cut(s) 706, 738
PstNI CAGNNNCTG 1 cut(s) 373
PsuI RGATCY 3 cut(s) 635, 1088, 1786
RsaI GTAC 4 cut(s) 425, 554, 610, 1863
RsaNI GTAC 4 cut(s) 424, 553, 609, 1862
RseI CAYNNNNRTG 2 cut(s) 587, 1687
SacI GAGCTC 1 cut(s) 849
SacII CCGCGG 1 cut(s) 228
SalI GTCGAC 1 cut(s) 385
SapI GCTCTTC 1 cut(s) 590
SaqAI TTAA 3 cut(s) 999, 1226, 1478
Sau3AI GATC 7 cut(s) 520, 547, 635, 682, 1088, 1531, 1786
Sau96I GGNCC 4 cut(s) 324, 836, 1368, 1495
SchI GAGTC 3 cut(s) 241, 428, 749
ScrFI CCNGG 5 cut(s) 64, 328, 913, 1421, 1493
SduI GDGCHC 2 cut(s) 849, 1160
SfaNI GCATC 6 cut(s) 228, 800, 995, 1112, 1656, 1688
Sfr274I CTCGAG 3 cut(s) 123, 1044, 1164
Sfr303I CCGCGG 1 cut(s) 228
SfuI TTCGAA 2 cut(s) 188, 1246
SgrAI CRCCGGYG 3 cut(s) 205, 344, 508
SgrBI CCGCGG 1 cut(s) 228
SgrDI CGTCGACG 1 cut(s) 385
SinI GGWCC 2 cut(s) 324, 1368
SlaI CTCGAG 3 cut(s) 123, 1044, 1164
SmiMI CAYNNNNRTG 2 cut(s) 587, 1687
SmlI CTYRAG 3 cut(s) 123, 1044, 1164
SmoI CTYRAG 3 cut(s) 123, 1044, 1164
SphI GCATGC 1 cut(s) 1766
SseBI AGGCCT 2 cut(s) 67, 1099
SspMI CTAG 5 cut(s) 891, 1095, 1338, 1355, 1581
SstI GAGCTC 1 cut(s) 849
StuI AGGCCT 2 cut(s) 67, 1099
StyD4I CCNGG 5 cut(s) 62, 326, 911, 1419, 1491
StyI CCWWGG 1 cut(s) 117
TaaI ACNGT 3 cut(s) 613, 777, 1801
TaiI ACGT 5 cut(s) 257, 386, 587, 1749, 1861
TauI GCSGC 7 cut(s) 41, 227, 230, 250, 293, 344, 365
TfiI GAWTC 2 cut(s) 28, 1424
Tru1I TTAA 3 cut(s) 999, 1226, 1478
Tru9I TTAA 3 cut(s) 999, 1226, 1478
TscAI CASTG 1 cut(s) 1605
TseFI GTSAC 1 cut(s) 130
TseI GCWGC 7 cut(s) 8, 338, 373, 1235, 1349, 1358, 1805
Tsp45I GTSAC 1 cut(s) 130
TspDTI ATGAA 2 cut(s) 765, 1749
TspGWI ACGGA 3 cut(s) 72, 189, 348
TspRI CASTG 1 cut(s) 1605
Van91I CCANNNNNTGG 1 cut(s) 1085
VpaK11BI GGWCC 2 cut(s) 324, 1368
XapI RAATTY 2 cut(s) 532, 1468
XceI RCATGY 3 cut(s) 148, 1289, 1766
XhoI CTCGAG 3 cut(s) 123, 1044, 1164
XmiI GTMKAC 1 cut(s) 386
XspI CTAG 5 cut(s) 891, 1095, 1338, 1355, 1581
Zsp2I ATGCAT 1 cut(s) 1399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.