Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0001429

Physical Location & Seq

Genomic Coordinates Reverse (-)

21917699 .. 21919180

Gene Structure 1482 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G54000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 810 bp

ATGAATCATGGAATTTCAGAAGCCTTGCTCGACAAGATTCACGAGCTAACGAAGCAGTTCTTTGTACTTCCGACCAAAGAGAAACAGAAGTACGCAAGAGAGATAAGTAGTTTTCAAGGATTTGGAAACGACATGATTCTGTCTGATGATCAAGTTCTCGATTGGGTCGACCGCTTGTACCTCATTACGTACCCTGAAGATCAACGACAGCTTAAATTCTGGCCTGAAAATCCAAGCGGATTTAGGGAAACTTTACATGAATACACAATGAAGCAACAGTTAGTGGTTGAGAAATTCTTCAAGGCATTAGCTAGATCGTTGGAGTTGGAAGATAATTGCTTTCTAGAGATGCATGGTGAAAATGCTACGTTGGAGACAAGATTCAACATCTACCCTCCATGTCCGAGGCCGGACAAGGTTCTTGGGCTAAAACCGCATTCTGATGGCTCAGCCTTTACTCTTATCCTACCCGACAAAAATGTAGAAGGACTTCAGTTCCTCAAAGATGGGAAGTGGTATAAAGCTTCAATTCTCCCTCATACAATTCTGATCAATGTAGGAGATACTATGGAGGTAATGAGCAATGGAATATACAAGAGTCCGGTTCATAGAGTGGTGCTTAACGGAAAAAAGGAAAGGATATATGTGGCAACATTTTGTAATGCTGATGAAGACAAAGAGATTCAACCTTTAAATGGGCTTGTGTCTGAGGCTAGACCAAGGTTGTACAAAGCAGTTAAGAAGTCTGAGAAAAACTTCTTCGATTACTATCAACAAGGTCGAAGACCGATCGAAGCTGCAATGATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0010150 GO:0016491 GO:0016679 GO:0016682 GO:0032501 GO:0032502 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0055114 GO:0090693 GO:0099402

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

269

Amino Acids

31.22

Weight (kDa)

6.41

Isoelectric Point (pI)

36.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	2 - 77	4.5e-15	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	127 - 221	1.2e-28	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001429)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G49390 AT1G49390 AT5G20400 AT5G20550 AT5G54000 AT5G54000
fragaria_vesca	FvH4_1g02121 FvH4_1g02150 FvH4_1g02151 FvH4_1g02152 FvH4_1g02160 FvH4_1g02160 FvH4_1g02160 FvH4_1g02160
malus_domestica	MD02G1019700.v1.1 MD02G1020000.v1.1 MD02G1020100.v1.1 MD14G1108700.v1.1 MD14G1108800.v1.1
prunus_persica	Prupe.4G248100_v2.0.a1 Prupe.4G248200_v2.0.a1 Prupe.4G248300_v2.0.a1 Prupe.4G248400_v2.0.a1 Prupe.4G248600_v2.0.a1
pyrus_communis	pycom02g01750 pycom02g01760
rosa_chinensis	RchiOBHm_Chr2g0087201 RchiOBHm_Chr2g0087211 RchiOBHm_Chr2g0087231 RchiOBHm_Chr2g0087261
rosa_laevigata	RLG00000015844 RLG00000015846 RLG00000015848
rosa_multiflora	Rmu_co8425197.1_g000001 Rmu_co8475215.1_g000001 Rmu_sc0003367.1_g000009 Rmu_sc0003367.1_g000015 Rmu_sc0018794.1_g000005
rosa_roxburghii	Rroxscaffold_2G00153770 Rroxscaffold_2G00153780 Rroxscaffold_2G00153810
rosa_rugosa	Rorug01G0473300 Rorug01G0473600
rosa_samantha	Rh2AG026600 Rh2AG026900 Rh2AG027100 Rh2BG026400 Rh2DG026700 Rh2DG026900 Rh2DG027100
rosa_wichuraiana	Rw2G002120 Rw2G002130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	168
AciI	CCGC	3 cut(s)	172, 237, 434
AcsI	RAATTY	3 cut(s)	12, 215, 293
AcuI	CTGAAG	2 cut(s)	216, 476
AfaI	GTAC	5 cut(s)	66, 92, 179, 191, 728
AfiI	CCNNNNNNNGG	1 cut(s)	695
AgsI	TTSAA	5 cut(s)	116, 301, 385, 528, 686
AluBI	AGCT	5 cut(s)	46, 211, 311, 524, 797
AluI	AGCT	5 cut(s)	46, 211, 311, 524, 797
Alw26I	GTCTC	1 cut(s)	368
AoxI	GGCC	2 cut(s)	221, 407
ApeKI	GCWGC	1 cut(s)	797
ApoI	RAATTY	3 cut(s)	12, 215, 293
Asp700I	GAANNNNTTC	4 cut(s)	56, 296, 489, 755
AsuHPI	GGTGA	1 cut(s)	368
BarI	GAAGNNNNNNTAC	2 cut(s)	474, 506
BauI	CACGAG	1 cut(s)	41
BbsI	GAAGAC	2 cut(s)	678, 790
BbvI	GCAGC	1 cut(s)	784
BccI	CCATC	2 cut(s)	437, 500
BclI	TGATCA	2 cut(s)	148, 549
BcoDI	GTCTC	1 cut(s)	368
BfaI	CTAG	3 cut(s)	312, 344, 714
BisI	GCNGC	1 cut(s)	798
BlpI	GCTNAGC	1 cut(s)	448
BlsI	GCNGC	1 cut(s)	799
BmsI	GCATC	1 cut(s)	339
BpiI	GAAGAC	2 cut(s)	678, 790
Bpu1102I	GCTNAGC	1 cut(s)	448
BsaAI	YACGTR	1 cut(s)	189
BsaBI	GATNNNNATC	1 cut(s)	768
BsaJI	CCNNGG	2 cut(s)	404, 719
BsaWI	WCCGGW	1 cut(s)	601
Bsc4I	CCNNNNNNNGG	1 cut(s)	695
Bse3DI	GCAATG	2 cut(s)	589, 807
Bse8I	GATNNNNATC	1 cut(s)	768
BseDI	CCNNGG	2 cut(s)	404, 719
BseJI	GATNNNNATC	1 cut(s)	768
BseLI	CCNNNNNNNGG	1 cut(s)	695
BseMI	GCAATG	2 cut(s)	589, 807
BseMII	CTCAG	3 cut(s)	462, 699, 738
BseXI	GCAGC	1 cut(s)	784
Bsh1285I	CGRYCG	2 cut(s)	172, 792
BshFI	GGCC	2 cut(s)	223, 409
BsiEI	CGRYCG	2 cut(s)	172, 792
BsiSI	CCGG	2 cut(s)	410, 602
BslI	CCNNNNNNNGG	1 cut(s)	695
BsmAI	GTCTC	1 cut(s)	368
BsmI	GAATGC	1 cut(s)	436
BsnI	GGCC	2 cut(s)	223, 409
Bsp1407I	TGTACA	1 cut(s)	726
Bsp143I	GATC	6 cut(s)	148, 199, 314, 549, 789, 804
Bsp1720I	GCTNAGC	1 cut(s)	448
BspACI	CCGC	3 cut(s)	172, 237, 434
BspANI	GGCC	2 cut(s)	223, 409
BspCNI	CTCAG	3 cut(s)	461, 700, 739
BsrDI	GCAATG	2 cut(s)	589, 807
BsrGI	TGTACA	1 cut(s)	726
BssECI	CCNNGG	2 cut(s)	404, 719
BssMI	GATC	6 cut(s)	148, 199, 314, 549, 789, 804
BssSI	CACGAG	1 cut(s)	41
BssT1I	CCWWGG	1 cut(s)	719
Bst2BI	CACGAG	1 cut(s)	41
Bst4CI	ACNGT	1 cut(s)	279
BstAUI	TGTACA	1 cut(s)	726
BstBAI	YACGTR	1 cut(s)	189
BstDEI	CTNAG	3 cut(s)	448, 708, 747
BstKTI	GATC	6 cut(s)	151, 202, 317, 552, 792, 807
BstMAI	GTCTC	1 cut(s)	368
BstMBI	GATC	6 cut(s)	148, 199, 314, 549, 789, 804
BstMCI	CGRYCG	2 cut(s)	172, 792
BstMWI	GCNNNNNNNGC	2 cut(s)	52, 433
BstSNI	TACGTA	1 cut(s)	189
BstV1I	GCAGC	1 cut(s)	784
BstV2I	GAAGAC	2 cut(s)	678, 790
BsuRI	GGCC	2 cut(s)	223, 409
Csp6I	GTAC	5 cut(s)	65, 91, 178, 190, 727
CviAII	CATG	5 cut(s)	8, 133, 257, 353, 399
CviQI	GTAC	5 cut(s)	65, 91, 178, 190, 727
DdeI	CTNAG	3 cut(s)	448, 708, 747
DpnI	GATC	6 cut(s)	150, 201, 316, 551, 791, 806
DpnII	GATC	6 cut(s)	148, 199, 314, 549, 789, 804
DraI	TTTAAA	1 cut(s)	693
Eco105I	TACGTA	1 cut(s)	189
Eco130I	CCWWGG	1 cut(s)	719
Eco57I	CTGAAG	2 cut(s)	216, 476
EcoT14I	CCWWGG	1 cut(s)	719
EcoT22I	ATGCAT	1 cut(s)	354
ErhI	CCWWGG	1 cut(s)	719
FaeI	CATG	5 cut(s)	11, 136, 260, 356, 402
FalI	AAGNNNNNCTT	2 cut(s)	44, 76
FatI	CATG	5 cut(s)	7, 132, 256, 352, 398
FbaI	TGATCA	2 cut(s)	148, 549
FblI	GTMKAC	1 cut(s)	168
Fnu4HI	GCNGC	1 cut(s)	798
Fsp4HI	GCNGC	1 cut(s)	798
FspBI	CTAG	3 cut(s)	312, 344, 714
GluI	GCNGC	1 cut(s)	798
HaeIII	GGCC	2 cut(s)	223, 409
HapII	CCGG	2 cut(s)	410, 602
Hin1II	CATG	5 cut(s)	11, 136, 260, 356, 402
HincII	GTYRAC	1 cut(s)	169
HindII	GTYRAC	1 cut(s)	169
HindIII	AAGCTT	1 cut(s)	522
HinfI	GANTC	6 cut(s)	4, 37, 136, 381, 598, 682
HpaII	CCGG	2 cut(s)	410, 602
HphI	GGTGA	1 cut(s)	368
Hpy166II	GTNNAC	1 cut(s)	169
Hpy188I	TCNGA	9 cut(s)	19, 72, 145, 405, 442, 549, 709, 748, 809
Hpy188III	TCNNGA	3 cut(s)	41, 158, 344
Hpy8I	GTNNAC	1 cut(s)	169
HpyAV	CCTTC	1 cut(s)	479
HpyCH4III	ACNGT	1 cut(s)	279
HpyCH4IV	ACGT	2 cut(s)	188, 368
HpyCH4V	TGCA	2 cut(s)	352, 800
HpyF10VI	GCNNNNNNNGC	2 cut(s)	52, 433
HpyF3I	CTNAG	3 cut(s)	448, 708, 747
HpySE526I	ACGT	2 cut(s)	188, 368
Hsp92II	CATG	5 cut(s)	11, 136, 260, 356, 402
Ksp22I	TGATCA	2 cut(s)	148, 549
Kzo9I	GATC	6 cut(s)	148, 199, 314, 549, 789, 804
LpnPI	CCDG	5 cut(s)	205, 207, 237, 423, 615
Lsp1109I	GCAGC	1 cut(s)	784
LweI	GCATC	1 cut(s)	339
MaeI	CTAG	3 cut(s)	312, 344, 714
MaeII	ACGT	2 cut(s)	188, 368
MalI	GATC	6 cut(s)	150, 201, 316, 551, 791, 806
MboI	GATC	6 cut(s)	148, 199, 314, 549, 789, 804
MboII	GAAGA	6 cut(s)	209, 289, 341, 683, 751, 795
MluCI	AATT	6 cut(s)	12, 215, 293, 334, 528, 543
MlyI	GAGTC	1 cut(s)	607
MmeI	TCCRAC	4 cut(s)	95, 300, 306, 351
MnlI	CCTC	7 cut(s)	191, 399, 405, 509, 546, 565, 703
Mph1103I	ATGCAT	1 cut(s)	354
MroXI	GAANNNNTTC	4 cut(s)	56, 296, 489, 755
MseI	TTAA	4 cut(s)	213, 621, 692, 738
MslI	CAYNNNNRTG	1 cut(s)	441
MspI	CCGG	2 cut(s)	410, 602
Mva1269I	GAATGC	1 cut(s)	436
MwoI	GCNNNNNNNGC	2 cut(s)	52, 433
NdeII	GATC	6 cut(s)	148, 199, 314, 549, 789, 804
NlaIII	CATG	5 cut(s)	11, 136, 260, 356, 402
NsiI	ATGCAT	1 cut(s)	354
PcsI	WCGNNNNNNNCGW	1 cut(s)	165
PctI	GAATGC	1 cut(s)	436
PdmI	GAANNNNTTC	4 cut(s)	56, 296, 489, 755
PfeI	GAWTC	5 cut(s)	4, 37, 136, 381, 682
PkrI	GCNGC	1 cut(s)	799
Ple19I	CGATCG	1 cut(s)	792
PleI	GAGTC	1 cut(s)	606
PpsI	GAGTC	1 cut(s)	606
Ppu21I	YACGTR	1 cut(s)	189
PvuI	CGATCG	1 cut(s)	792
RsaI	GTAC	5 cut(s)	66, 92, 179, 191, 728
RsaNI	GTAC	5 cut(s)	65, 91, 178, 190, 727
RseI	CAYNNNNRTG	1 cut(s)	441
SalI	GTCGAC	1 cut(s)	167
SaqAI	TTAA	4 cut(s)	213, 621, 692, 738
SatI	GCNGC	1 cut(s)	798
Sau3AI	GATC	6 cut(s)	148, 199, 314, 549, 789, 804
SchI	GAGTC	1 cut(s)	607
SfaNI	GCATC	1 cut(s)	339
SmiMI	CAYNNNNRTG	1 cut(s)	441
SnaBI	TACGTA	1 cut(s)	189
Sse9I	AATT	6 cut(s)	12, 215, 293, 334, 528, 543
SsiI	CCGC	3 cut(s)	172, 237, 434
SspMI	CTAG	3 cut(s)	312, 344, 714
StyI	CCWWGG	1 cut(s)	719
TaaI	ACNGT	1 cut(s)	279
TaiI	ACGT	2 cut(s)	191, 371
TaqI	TCGA	6 cut(s)	30, 159, 168, 762, 781, 792
TaqII	GACCGA	1 cut(s)	802
TasI	AATT	6 cut(s)	12, 215, 293, 334, 528, 543
TatI	WGTACW	2 cut(s)	64, 726
TfiI	GAWTC	5 cut(s)	4, 37, 136, 381, 682
Tru1I	TTAA	4 cut(s)	213, 621, 692, 738
Tru9I	TTAA	4 cut(s)	213, 621, 692, 738
TseI	GCWGC	1 cut(s)	797
TspDTI	ATGAA	5 cut(s)	17, 273, 284, 596, 684
TspGWI	ACGGA	1 cut(s)	639
XapI	RAATTY	3 cut(s)	12, 215, 293
XbaI	TCTAGA	1 cut(s)	343
XmiI	GTMKAC	1 cut(s)	168
XmnI	GAANNNNTTC	4 cut(s)	56, 296, 489, 755
XspI	CTAG	3 cut(s)	312, 344, 714
Zsp2I	ATGCAT	1 cut(s)	354

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.