Prupe.4G248300_v2.0.a1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0001429
Physical Location & Seq
Forward (+)
16572312 .. 16574906
2595 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G248300.1

Sequence Viewer

Length: 1092 bp
ATGGACATGGCTGAGTCTTCCACTATTGCCAAGATAGAGTTAGGATCCACAACTGTCCAAGAGCTGCTCGCCAAAGGCAAAGGCAAAGGGGAACAAGTGCCAGATAAATATATCCATAAAGTTACAGACGGTTGCATTGGAGCCCCAAGCGCTTCTTCTGCCGCGTTGATGGATATTCCAGTCATTGATCTTGGCCTCCCCCTCACACCTTCCTCAATCACTGCACAACAACTTGATAAACTTCGATCAGCTCTTACCACATGGGGTTGCTTTCAGGTAATAAACCATGGCATGACTCTTGAATTCCTAGACAAGGTTCGCGAAATGGCAAAACAATTTTTTGCACTTCCAGTGGAAGAGAAGCAGAACTACTTGAGGCAAGTCAACGATACTGAAGGATATGGGAGCGACATGGTTTTTTCAGAACAACAAACACTTGATTGGACTGACCGGCTATACCTTTCTGTATATCCAGAAGACCGCCGAAAGTTCAAATTTTGGCCTCAAAACCCCAAATCTTTTAGTGAAACTTTAGACCAATATACGATGAAGTTACAAGTGGTAACAAAAACTGTCCTGGAGGCCATGGCAAGGTTATTGAATTTGGATGATAATTGCTTTCGGGACCTGTATGTAGAACACGGAAAAATGGATGTGAGGTTTAACTTGTATCCCCCGTGTTCAAGGCCTGATGTCGTCCTGGGTGTGAAACCGCATGCGGATGGAACAATAATCACCCTTCTCTTGCAAGACAAACAAGTCGAAGGTCTTCAGTTTCTTAAAGACGATCAGTGGTTTAGATCTCCCATTGTTCCTGAGGCGCTTCTCATTAATGTTGGTGATCAGGCAGAGATATTGAGCAATGGAAAATTCAAGAGCCCTATACACAGGGTAGTGATAAATCCAGACAAAGAGAGGATCTCTTTGGCTGCCTTCTGCATCCCTGAATCAGATAAAGAGATTGAACCCTTTGAAAGCCTGGTAAATGAGTCAACGCCGAGATTATACAAAATGGTGAAAGATTATTCTGGCGTCTTTTTCGAATACCACTACCAGCAGGGGAGGAGACCAATAGAAGCAGCAAAAATTTGA

Protein Analysis

364

Amino Acids

41.24

Weight (kDa)

5.34

Isoelectric Point (pI)

41.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 758
AccII CGCG 2 cut(s) 164, 321
AciI CCGC 4 cut(s) 162, 481, 713, 719
AclWI GGATC 3 cut(s) 39, 52, 926
AcsI RAATTY 5 cut(s) 302, 494, 601, 869, 1086
AcuI CTGAAG 2 cut(s) 414, 755
AcyI GRCGYC 1 cut(s) 1032
AfeI AGCGCT 1 cut(s) 151
AfiI CCNNNNNNNGG 2 cut(s) 313, 591
AgsI TTSAA 7 cut(s) 302, 493, 601, 684, 874, 965, 974
AjnI CCWGG 3 cut(s) 576, 699, 978
AjuI GAANNNNNNNTTGG 2 cut(s) 139, 171
AluBI AGCT 2 cut(s) 64, 251
AluI AGCT 2 cut(s) 64, 251
Alw26I GTCTC 1 cut(s) 1060
AlwI GGATC 3 cut(s) 39, 52, 926
Aor51HI AGCGCT 1 cut(s) 151
AoxI GGCC 4 cut(s) 193, 500, 582, 686
ApeKI GCWGC 3 cut(s) 64, 929, 1079
ApoI RAATTY 5 cut(s) 302, 494, 601, 869, 1086
AseI ATTAAT 1 cut(s) 831
Asp700I GAANNNNTTC 1 cut(s) 768
AspLEI GCGC 2 cut(s) 152, 823
AspS9I GGNCC 1 cut(s) 625
AsuHPI GGTGA 3 cut(s) 727, 851, 1027
AsuII TTCGAA 1 cut(s) 1041
AvaII GGWCC 1 cut(s) 625
AxyI CCTNAGG 1 cut(s) 816
BamHI GGATCC 1 cut(s) 44
BanII GRGCYC 2 cut(s) 145, 881
BarI GAAGNNNNNNTAC 2 cut(s) 353, 385
BbsI GAAGAC 3 cut(s) 9, 483, 761
BbvI GCAGC 2 cut(s) 51, 916
BccI CCATC 2 cut(s) 163, 716
BciT130I CCWGG 3 cut(s) 578, 701, 980
BciVI GTATCC 1 cut(s) 681
BclI TGATCA 1 cut(s) 841
BcoDI GTCTC 1 cut(s) 1060
BfaI CTAG 1 cut(s) 308
BfoI RGCGCY 2 cut(s) 153, 824
BfuI GTATCC 1 cut(s) 681
BglII AGATCT 1 cut(s) 800
BisI GCNGC 4 cut(s) 65, 162, 930, 1080
BlsI GCNGC 4 cut(s) 66, 163, 931, 1081
Bme1390I CCNGG 3 cut(s) 578, 701, 980
Bme18I GGWCC 1 cut(s) 625
BmgT120I GGNCC 1 cut(s) 625
BmiI GGNNCC 3 cut(s) 46, 142, 626
BmrFI CCNGG 3 cut(s) 578, 701, 980
BmsI GCATC 1 cut(s) 948
BpiI GAAGAC 3 cut(s) 9, 483, 761
BplI GAGNNNNNCTC 2 cut(s) 905, 937
BpmI CTGGAG 1 cut(s) 599
Bpu14I TTCGAA 1 cut(s) 1041
BpuEI CTTGAG 1 cut(s) 394
BsaHI GRCGYC 1 cut(s) 1032
BsaI GGTCTC 1 cut(s) 1060
BsaJI CCNNGG 3 cut(s) 286, 585, 700
Bsc4I CCNNNNNNNGG 2 cut(s) 313, 591
Bse118I RCCGGY 1 cut(s) 450
Bse1I ACTGG 2 cut(s) 179, 350
Bse21I CCTNAGG 1 cut(s) 816
Bse3DI GCAATG 1 cut(s) 868
BseBI CCWGG 3 cut(s) 578, 701, 980
BseDI CCNNGG 3 cut(s) 286, 585, 700
BseGI GGATG 4 cut(s) 613, 658, 727, 939
BseLI CCNNNNNNNGG 2 cut(s) 313, 591
BseMI GCAATG 1 cut(s) 868
BseMII CTCAG 1 cut(s) 807
BseNI ACTGG 2 cut(s) 179, 350
BseRI GAGGAG 1 cut(s) 1078
BseXI GCAGC 2 cut(s) 51, 916
BsgI GTGCAG 1 cut(s) 207
Bsh1236I CGCG 2 cut(s) 164, 321
BshFI GGCC 4 cut(s) 195, 502, 584, 688
BsiSI CCGG 1 cut(s) 451
BslFI GGGAC 1 cut(s) 638
BslI CCNNNNNNNGG 2 cut(s) 313, 591
BsmAI GTCTC 1 cut(s) 1060
BsmFI GGGAC 1 cut(s) 638
BsnI GGCC 4 cut(s) 195, 502, 584, 688
Bso31I GGTCTC 1 cut(s) 1060
Bsp119I TTCGAA 1 cut(s) 1041
Bsp1286I GDGCHC 2 cut(s) 145, 881
Bsp143I GATC 7 cut(s) 44, 187, 245, 787, 800, 841, 918
Bsp19I CCATGG 2 cut(s) 286, 585
Bsp68I TCGCGA 1 cut(s) 321
BspACI CCGC 4 cut(s) 162, 481, 713, 719
BspANI GGCC 4 cut(s) 195, 502, 584, 688
BspCNI CTCAG 2 cut(s) 4, 808
BspFNI CGCG 2 cut(s) 164, 321
BspLI GGNNCC 3 cut(s) 46, 142, 626
BspPI GGATC 3 cut(s) 39, 52, 926
BspT104I TTCGAA 1 cut(s) 1041
BspTNI GGTCTC 1 cut(s) 1060
BsrDI GCAATG 1 cut(s) 868
BsrFI RCCGGY 1 cut(s) 450
BsrI ACTGG 2 cut(s) 179, 350
BssAI RCCGGY 1 cut(s) 450
BssECI CCNNGG 3 cut(s) 286, 585, 700
BssMI GATC 7 cut(s) 44, 187, 245, 787, 800, 841, 918
BssNI GRCGYC 1 cut(s) 1032
BssT1I CCWWGG 2 cut(s) 286, 585
Bst2UI CCWGG 3 cut(s) 578, 701, 980
Bst4CI ACNGT 3 cut(s) 55, 131, 574
Bst6I CTCTTC 1 cut(s) 351
BstACI GRCGYC 1 cut(s) 1032
BstBI TTCGAA 1 cut(s) 1041
BstC8I GCNNGC 2 cut(s) 69, 717
BstDEI CTNAG 2 cut(s) 12, 816
BstDSI CCRYGG 2 cut(s) 286, 585
BstENI CCTNNNNNAGG 1 cut(s) 311
BstF5I GGATG 4 cut(s) 613, 658, 727, 939
BstFNI CGCG 2 cut(s) 164, 321
BstH2I RGCGCY 2 cut(s) 153, 824
BstHHI GCGC 2 cut(s) 152, 823
BstKTI GATC 7 cut(s) 47, 190, 248, 790, 803, 844, 921
BstMAI GTCTC 1 cut(s) 1060
BstMBI GATC 7 cut(s) 44, 187, 245, 787, 800, 841, 918
BstMWI GCNNNNNNNGC 2 cut(s) 149, 158
BstNI CCWGG 3 cut(s) 578, 701, 980
BstNSI RCATGY 1 cut(s) 719
BstSCI CCNGG 3 cut(s) 576, 699, 978
BstUI CGCG 2 cut(s) 164, 321
BstV1I GCAGC 2 cut(s) 51, 916
BstV2I GAAGAC 3 cut(s) 9, 483, 761
BstX2I RGATCY 3 cut(s) 44, 800, 918
BstYI RGATCY 3 cut(s) 44, 800, 918
Bsu36I CCTNAGG 1 cut(s) 816
BsuI GTATCC 1 cut(s) 681
BsuRI GGCC 4 cut(s) 195, 502, 584, 688
BtgI CCRYGG 2 cut(s) 286, 585
BtsCI GGATG 4 cut(s) 613, 658, 727, 939
BtsI GCAGTG 1 cut(s) 219
BtsIMutI CAGTG 3 cut(s) 219, 357, 797
BtuMI TCGCGA 1 cut(s) 321
Cac8I GCNNGC 2 cut(s) 69, 717
CfoI GCGC 2 cut(s) 152, 823
Cfr10I RCCGGY 1 cut(s) 450
Cfr13I GGNCC 1 cut(s) 625
CseI GACGC 1 cut(s) 1021
CviAII CATG 7 cut(s) 7, 261, 287, 292, 412, 586, 716
DdeI CTNAG 2 cut(s) 12, 816
DpnI GATC 7 cut(s) 46, 189, 247, 789, 802, 843, 920
DpnII GATC 7 cut(s) 44, 187, 245, 787, 800, 841, 918
DrdI GACNNNNNNGTC 1 cut(s) 758
DseDI GACNNNNNNGTC 1 cut(s) 758
Eam1104I CTCTTC 1 cut(s) 351
EarI CTCTTC 1 cut(s) 351
Eco130I CCWWGG 2 cut(s) 286, 585
Eco147I AGGCCT 1 cut(s) 688
Eco24I GRGCYC 2 cut(s) 145, 881
Eco31I GGTCTC 1 cut(s) 1060
Eco47I GGWCC 1 cut(s) 625
Eco47III AGCGCT 1 cut(s) 151
Eco57I CTGAAG 2 cut(s) 414, 755
Eco81I CCTNAGG 1 cut(s) 816
EcoNI CCTNNNNNAGG 1 cut(s) 311
EcoO109I RGGNCCY 1 cut(s) 625
EcoRI GAATTC 1 cut(s) 302
EcoRII CCWGG 3 cut(s) 576, 699, 978
EcoT14I CCWWGG 2 cut(s) 286, 585
EcoT38I GRGCYC 2 cut(s) 145, 881
ErhI CCWWGG 2 cut(s) 286, 585
FaeI CATG 7 cut(s) 10, 264, 290, 295, 415, 589, 719
FalI AAGNNNNNCTT 2 cut(s) 139, 171
FaqI GGGAC 1 cut(s) 638
FatI CATG 7 cut(s) 6, 260, 286, 291, 411, 585, 715
FbaI TGATCA 1 cut(s) 841
Fnu4HI GCNGC 4 cut(s) 65, 162, 930, 1080
FokI GGATG 4 cut(s) 620, 665, 734, 926
FriOI GRGCYC 2 cut(s) 145, 881
Fsp4HI GCNGC 4 cut(s) 65, 162, 930, 1080
FspBI CTAG 1 cut(s) 308
GlaI GCGC 2 cut(s) 151, 822
GluI GCNGC 4 cut(s) 65, 162, 930, 1080
GsuI CTGGAG 1 cut(s) 599
HaeII RGCGCY 2 cut(s) 153, 824
HaeIII GGCC 4 cut(s) 195, 502, 584, 688
HapII CCGG 1 cut(s) 451
HgaI GACGC 1 cut(s) 1021
HhaI GCGC 2 cut(s) 152, 823
Hin1I GRCGYC 1 cut(s) 1032
Hin1II CATG 7 cut(s) 10, 264, 290, 295, 415, 589, 719
Hin6I GCGC 2 cut(s) 150, 821
HinP1I GCGC 2 cut(s) 150, 821
HincII GTYRAC 2 cut(s) 385, 993
HindII GTYRAC 2 cut(s) 385, 993
HinfI GANTC 4 cut(s) 14, 295, 947, 989
HpaII CCGG 1 cut(s) 451
HphI GGTGA 3 cut(s) 727, 851, 1027
Hpy166II GTNNAC 2 cut(s) 385, 993
Hpy188I TCNGA 2 cut(s) 424, 952
Hpy188III TCNNGA 7 cut(s) 299, 320, 473, 623, 815, 874, 905
Hpy8I GTNNAC 2 cut(s) 385, 993
HpyAV CCTTC 5 cut(s) 219, 389, 749, 758, 943
HpyCH4III ACNGT 3 cut(s) 55, 131, 574
HpyCH4V TGCA 5 cut(s) 135, 224, 344, 748, 939
HpyF10VI GCNNNNNNNGC 2 cut(s) 149, 158
HpyF3I CTNAG 2 cut(s) 12, 816
Hsp92I GRCGYC 1 cut(s) 1032
Hsp92II CATG 7 cut(s) 10, 264, 290, 295, 415, 589, 719
HspAI GCGC 2 cut(s) 150, 821
Ksp22I TGATCA 1 cut(s) 841
Kzo9I GATC 7 cut(s) 44, 187, 245, 787, 800, 841, 918
LmnI GCTCC 2 cut(s) 140, 405
Lsp1109I GCAGC 2 cut(s) 51, 916
LweI GCATC 1 cut(s) 948
MaeI CTAG 1 cut(s) 308
MaeIII GTNAC 3 cut(s) 121, 552, 562
MalI GATC 7 cut(s) 46, 189, 247, 789, 802, 843, 920
MboI GATC 7 cut(s) 44, 187, 245, 787, 800, 841, 918
MboII GAAGA 5 cut(s) 9, 147, 368, 488, 761
MflI RGATCY 3 cut(s) 44, 800, 918
MhlI GDGCHC 2 cut(s) 145, 881
MluCI AATT 7 cut(s) 302, 335, 494, 601, 613, 869, 1086
MlyI GAGTC 3 cut(s) 23, 289, 998
MroXI GAANNNNTTC 1 cut(s) 768
MseI TTAA 3 cut(s) 663, 780, 831
MslI CAYNNNNRTG 1 cut(s) 720
MspI CCGG 1 cut(s) 451
MspR9I CCNGG 3 cut(s) 578, 701, 980
MvaI CCWGG 3 cut(s) 578, 701, 980
MvnI CGCG 2 cut(s) 164, 321
MwoI GCNNNNNNNGC 2 cut(s) 149, 158
NcoI CCATGG 2 cut(s) 286, 585
NdeII GATC 7 cut(s) 44, 187, 245, 787, 800, 841, 918
NlaIII CATG 7 cut(s) 10, 264, 290, 295, 415, 589, 719
NlaIV GGNNCC 3 cut(s) 46, 142, 626
NmeAIII GCCGAG 1 cut(s) 1023
NruI TCGCGA 1 cut(s) 321
NspI RCATGY 1 cut(s) 719
NspV TTCGAA 1 cut(s) 1041
PaeI GCATGC 1 cut(s) 719
PceI AGGCCT 1 cut(s) 688
PdmI GAANNNNTTC 1 cut(s) 768
PfeI GAWTC 1 cut(s) 947
PfoI TCCNGGA 1 cut(s) 576
PkrI GCNGC 4 cut(s) 66, 163, 931, 1081
PleI GAGTC 3 cut(s) 22, 289, 997
PpsI GAGTC 3 cut(s) 22, 289, 997
PpuMI RGGWCCY 1 cut(s) 625
PshBI ATTAAT 1 cut(s) 831
Psp5II RGGWCCY 1 cut(s) 625
Psp6I CCWGG 3 cut(s) 576, 699, 978
PspGI CCWGG 3 cut(s) 576, 699, 978
PspN4I GGNNCC 3 cut(s) 46, 142, 626
PspPI GGNCC 1 cut(s) 625
PspPPI RGGWCCY 1 cut(s) 625
PsuI RGATCY 3 cut(s) 44, 800, 918
RruI TCGCGA 1 cut(s) 321
RseI CAYNNNNRTG 1 cut(s) 720
SaqAI TTAA 3 cut(s) 663, 780, 831
SatI GCNGC 4 cut(s) 65, 162, 930, 1080
Sau3AI GATC 7 cut(s) 44, 187, 245, 787, 800, 841, 918
Sau96I GGNCC 1 cut(s) 625
SchI GAGTC 3 cut(s) 23, 289, 998
ScrFI CCNGG 3 cut(s) 578, 701, 980
SduI GDGCHC 2 cut(s) 145, 881
SfaNI GCATC 1 cut(s) 948
SfuI TTCGAA 1 cut(s) 1041
SinI GGWCC 1 cut(s) 625
SmiMI CAYNNNNRTG 1 cut(s) 720
SmlI CTYRAG 1 cut(s) 373
SmoI CTYRAG 1 cut(s) 373
SphI GCATGC 1 cut(s) 719
Sse9I AATT 7 cut(s) 302, 335, 494, 601, 613, 869, 1086
SseBI AGGCCT 1 cut(s) 688
SsiI CCGC 4 cut(s) 162, 481, 713, 719
SspMI CTAG 1 cut(s) 308
StuI AGGCCT 1 cut(s) 688
StyD4I CCNGG 3 cut(s) 576, 699, 978
StyI CCWWGG 2 cut(s) 286, 585
TaaI ACNGT 3 cut(s) 55, 131, 574
TaqI TCGA 3 cut(s) 244, 762, 1041
TasI AATT 7 cut(s) 302, 335, 494, 601, 613, 869, 1086
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 1 cut(s) 947
Tru1I TTAA 3 cut(s) 663, 780, 831
Tru9I TTAA 3 cut(s) 663, 780, 831
TscAI CASTG 3 cut(s) 226, 357, 797
TseI GCWGC 3 cut(s) 64, 929, 1079
TspDTI ATGAA 1 cut(s) 563
TspGWI ACGGA 1 cut(s) 657
TspRI CASTG 3 cut(s) 226, 357, 797
VpaK11BI GGWCC 1 cut(s) 625
VspI ATTAAT 1 cut(s) 831
XagI CCTNNNNNAGG 1 cut(s) 311
XapI RAATTY 5 cut(s) 302, 494, 601, 869, 1086
XceI RCATGY 1 cut(s) 719
XmnI GAANNNNTTC 1 cut(s) 768
XspI CTAG 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.