Belongs to the iron ascorbate-dependent oxidoreductase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 58 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 130 |
| AccII | CGCG | 1 cut(s) | 770 |
| AciI | CCGC | 4 cut(s) | 442, 484, 878, 893 |
| AclI | AACGTT | 1 cut(s) | 454 |
| AcsI | RAATTY | 3 cut(s) | 188, 197, 260 |
| AdeI | CACNNNGTG | 1 cut(s) | 841 |
| AfaI | GTAC | 4 cut(s) | 329, 834, 902, 953 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 106, 130, 549 |
| AjiI | CACGTC | 2 cut(s) | 271, 370 |
| AjnI | CCWGG | 2 cut(s) | 30, 231 |
| AluBI | AGCT | 4 cut(s) | 167, 209, 448, 752 |
| AluI | AGCT | 4 cut(s) | 167, 209, 448, 752 |
| Alw21I | GWGCWC | 3 cut(s) | 42, 570, 754 |
| Alw26I | GTCTC | 1 cut(s) | 328 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 130 |
| AoxI | GGCC | 4 cut(s) | 458, 540, 635, 941 |
| ApeKI | GCWGC | 1 cut(s) | 164 |
| ApoI | RAATTY | 3 cut(s) | 188, 197, 260 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 71, 717 |
| AspLEI | GCGC | 3 cut(s) | 256, 772, 830 |
| AspS9I | GGNCC | 2 cut(s) | 274, 493 |
| AsuC2I | CCSGG | 1 cut(s) | 107 |
| AsuHPI | GGTGA | 3 cut(s) | 676, 746, 856 |
| AvaII | GGWCC | 2 cut(s) | 274, 493 |
| BanI | GGYRCC | 1 cut(s) | 58 |
| BanII | GRGCYC | 1 cut(s) | 754 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 702, 734 |
| BbsI | GAAGAC | 1 cut(s) | 710 |
| Bbv12I | GWGCWC | 3 cut(s) | 42, 570, 754 |
| BbvI | GCAGC | 1 cut(s) | 176 |
| BccI | CCATC | 2 cut(s) | 83, 955 |
| BceAI | ACGGC | 1 cut(s) | 262 |
| BciT130I | CCWGG | 2 cut(s) | 32, 233 |
| BcnI | CCSGG | 1 cut(s) | 107 |
| BcoDI | GTCTC | 1 cut(s) | 328 |
| BfmI | CTRYAG | 1 cut(s) | 180 |
| BfoI | RGCGCY | 1 cut(s) | 831 |
| BisI | GCNGC | 2 cut(s) | 165, 879 |
| BlsI | GCNGC | 2 cut(s) | 166, 880 |
| BmcAI | AGTACT | 1 cut(s) | 329 |
| Bme1390I | CCNGG | 3 cut(s) | 32, 107, 233 |
| Bme18I | GGWCC | 2 cut(s) | 274, 493 |
| BmgBI | CACGTC | 2 cut(s) | 271, 370 |
| BmgT120I | GGNCC | 2 cut(s) | 274, 493 |
| BmiI | GGNNCC | 1 cut(s) | 60 |
| BmrFI | CCNGG | 3 cut(s) | 32, 107, 233 |
| BmrI | ACTGGG | 2 cut(s) | 95, 118 |
| BmsI | GCATC | 1 cut(s) | 8 |
| BmuI | ACTGGG | 2 cut(s) | 95, 118 |
| BpiI | GAAGAC | 1 cut(s) | 710 |
| BpuEI | CTTGAG | 2 cut(s) | 352, 521 |
| BpuMI | CCSGG | 1 cut(s) | 107 |
| BsaJI | CCNNGG | 3 cut(s) | 244, 504, 632 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 87, 117 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 106, 130, 549 |
| Bse1I | ACTGG | 6 cut(s) | 101, 124, 135, 140, 308, 399 |
| Bse3DI | GCAATG | 1 cut(s) | 249 |
| BseBI | CCWGG | 2 cut(s) | 32, 233 |
| BseDI | CCNNGG | 3 cut(s) | 244, 504, 632 |
| BseGI | GGATG | 6 cut(s) | 23, 42, 115, 139, 674, 850 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 106, 130, 549 |
| BseMI | GCAATG | 1 cut(s) | 249 |
| BseMII | CTCAG | 1 cut(s) | 681 |
| BseNI | ACTGG | 6 cut(s) | 101, 124, 135, 140, 308, 399 |
| BseRI | GAGGAG | 4 cut(s) | 20, 108, 149, 1021 |
| BseXI | GCAGC | 1 cut(s) | 176 |
| Bsh1236I | CGCG | 1 cut(s) | 770 |
| BshFI | GGCC | 4 cut(s) | 460, 542, 637, 943 |
| BshNI | GGYRCC | 1 cut(s) | 58 |
| BsiHKAI | GWGCWC | 3 cut(s) | 42, 570, 754 |
| BsiSI | CCGG | 2 cut(s) | 107, 257 |
| BslFI | GGGAC | 3 cut(s) | 83, 89, 989 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 106, 130, 549 |
| BsmAI | GTCTC | 1 cut(s) | 328 |
| BsmFI | GGGAC | 3 cut(s) | 83, 89, 989 |
| BsmI | GAATGC | 1 cut(s) | 881 |
| BsnI | GGCC | 4 cut(s) | 460, 542, 637, 943 |
| Bsp1286I | GDGCHC | 3 cut(s) | 42, 570, 754 |
| Bsp1407I | TGTACA | 2 cut(s) | 832, 951 |
| Bsp143I | GATC | 3 cut(s) | 148, 203, 640 |
| BspACI | CCGC | 4 cut(s) | 442, 484, 878, 893 |
| BspANI | GGCC | 4 cut(s) | 460, 542, 637, 943 |
| BspCNI | CTCAG | 1 cut(s) | 680 |
| BspFNI | CGCG | 1 cut(s) | 770 |
| BspLI | GGNNCC | 1 cut(s) | 60 |
| BspMAI | CTGCAG | 1 cut(s) | 184 |
| BspT107I | GGYRCC | 1 cut(s) | 58 |
| BsrDI | GCAATG | 1 cut(s) | 249 |
| BsrGI | TGTACA | 2 cut(s) | 832, 951 |
| BsrI | ACTGG | 6 cut(s) | 101, 124, 135, 140, 308, 399 |
| BssECI | CCNNGG | 3 cut(s) | 244, 504, 632 |
| BssMI | GATC | 3 cut(s) | 148, 203, 640 |
| Bst2UI | CCWGG | 2 cut(s) | 32, 233 |
| Bst4CI | ACNGT | 4 cut(s) | 28, 294, 424, 532 |
| BstAUI | TGTACA | 2 cut(s) | 832, 951 |
| BstC8I | GCNNGC | 1 cut(s) | 446 |
| BstDEI | CTNAG | 2 cut(s) | 265, 667 |
| BstDSI | CCRYGG | 2 cut(s) | 244, 504 |
| BstF5I | GGATG | 6 cut(s) | 23, 42, 115, 139, 674, 850 |
| BstFNI | CGCG | 1 cut(s) | 770 |
| BstH2I | RGCGCY | 1 cut(s) | 831 |
| BstHHI | GCGC | 3 cut(s) | 256, 772, 830 |
| BstKTI | GATC | 3 cut(s) | 151, 206, 643 |
| BstMAI | GTCTC | 1 cut(s) | 328 |
| BstMBI | GATC | 3 cut(s) | 148, 203, 640 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 161 |
| BstNI | CCWGG | 2 cut(s) | 32, 233 |
| BstSCI | CCNGG | 3 cut(s) | 30, 105, 231 |
| BstSFI | CTRYAG | 1 cut(s) | 180 |
| BstUI | CGCG | 1 cut(s) | 770 |
| BstV1I | GCAGC | 1 cut(s) | 176 |
| BstV2I | GAAGAC | 1 cut(s) | 710 |
| BsuRI | GGCC | 4 cut(s) | 460, 542, 637, 943 |
| BtgI | CCRYGG | 2 cut(s) | 244, 504 |
| BtrI | CACGTC | 2 cut(s) | 271, 370 |
| BtsCI | GGATG | 6 cut(s) | 23, 42, 115, 139, 674, 850 |
| Cac8I | GCNNGC | 1 cut(s) | 446 |
| CaiI | CAGNNNCTG | 1 cut(s) | 130 |
| CfoI | GCGC | 3 cut(s) | 256, 772, 830 |
| Cfr13I | GGNCC | 2 cut(s) | 274, 493 |
| CseI | GACGC | 1 cut(s) | 776 |
| Csp6I | GTAC | 4 cut(s) | 328, 833, 901, 952 |
| CviAII | CATG | 4 cut(s) | 544, 598, 627, 689 |
| CviJI | RGCY | 9 cut(s) | 167, 209, 448, 460, 542, 561, 637, 752, 943 |
| CviKI_1 | RGCY | 9 cut(s) | 167, 209, 448, 460, 542, 561, 637, 752, 943 |
| CviQI | GTAC | 4 cut(s) | 328, 833, 901, 952 |
| DdeI | CTNAG | 2 cut(s) | 265, 667 |
| DpnI | GATC | 3 cut(s) | 150, 205, 642 |
| DpnII | GATC | 3 cut(s) | 148, 203, 640 |
| DraIII | CACNNNGTG | 1 cut(s) | 841 |
| EciI | GGCGGA | 1 cut(s) | 499 |
| Ecl136II | GAGCTC | 1 cut(s) | 752 |
| Eco147I | AGGCCT | 1 cut(s) | 637 |
| Eco24I | GRGCYC | 1 cut(s) | 754 |
| Eco32I | GATATC | 2 cut(s) | 604, 870 |
| Eco47I | GGWCC | 2 cut(s) | 274, 493 |
| Eco53kI | GAGCTC | 1 cut(s) | 752 |
| EcoICRI | GAGCTC | 1 cut(s) | 752 |
| EcoRI | GAATTC | 1 cut(s) | 260 |
| EcoRII | CCWGG | 2 cut(s) | 30, 231 |
| EcoRV | GATATC | 2 cut(s) | 604, 870 |
| EcoT38I | GRGCYC | 1 cut(s) | 754 |
| FaeI | CATG | 4 cut(s) | 547, 601, 630, 692 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 314, 346 |
| FaqI | GGGAC | 3 cut(s) | 83, 89, 989 |
| FatI | CATG | 4 cut(s) | 543, 597, 626, 688 |
| Fnu4HI | GCNGC | 2 cut(s) | 165, 879 |
| FokI | GGATG | 6 cut(s) | 29, 30, 122, 146, 661, 837 |
| FriOI | GRGCYC | 1 cut(s) | 754 |
| Fsp4HI | GCNGC | 2 cut(s) | 165, 879 |
| GlaI | GCGC | 3 cut(s) | 255, 771, 829 |
| GluI | GCNGC | 2 cut(s) | 165, 879 |
| HaeII | RGCGCY | 1 cut(s) | 831 |
| HaeIII | GGCC | 4 cut(s) | 460, 542, 637, 943 |
| HapII | CCGG | 2 cut(s) | 107, 257 |
| HgaI | GACGC | 1 cut(s) | 776 |
| HhaI | GCGC | 3 cut(s) | 256, 772, 830 |
| Hin1II | CATG | 4 cut(s) | 547, 601, 630, 692 |
| Hin6I | GCGC | 3 cut(s) | 254, 770, 828 |
| HinP1I | GCGC | 3 cut(s) | 254, 770, 828 |
| HinfI | GANTC | 6 cut(s) | 96, 113, 473, 609, 671, 896 |
| HpaII | CCGG | 2 cut(s) | 107, 257 |
| HphI | GGTGA | 3 cut(s) | 676, 746, 856 |
| Hpy166II | GTNNAC | 1 cut(s) | 835 |
| Hpy188I | TCNGA | 8 cut(s) | 17, 203, 278, 583, 608, 633, 670, 999 |
| Hpy188III | TCNNGA | 4 cut(s) | 212, 470, 764, 854 |
| Hpy8I | GTNNAC | 1 cut(s) | 835 |
| Hpy99I | CGWCG | 2 cut(s) | 350, 371 |
| HpyAV | CCTTC | 1 cut(s) | 707 |
| HpyCH4III | ACNGT | 4 cut(s) | 28, 294, 424, 532 |
| HpyCH4IV | ACGT | 4 cut(s) | 270, 348, 369, 454 |
| HpyCH4V | TGCA | 4 cut(s) | 21, 182, 302, 697 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 161 |
| HpyF3I | CTNAG | 2 cut(s) | 265, 667 |
| HpySE526I | ACGT | 4 cut(s) | 270, 348, 369, 454 |
| Hsp92II | CATG | 4 cut(s) | 547, 601, 630, 692 |
| HspAI | GCGC | 3 cut(s) | 254, 770, 828 |
| Kzo9I | GATC | 3 cut(s) | 148, 203, 640 |
| LmnI | GCTCC | 4 cut(s) | 382, 565, 594, 757 |
| Lsp1109I | GCAGC | 1 cut(s) | 176 |
| LweI | GCATC | 1 cut(s) | 8 |
| MaeII | ACGT | 4 cut(s) | 270, 348, 369, 454 |
| MaeIII | GTNAC | 2 cut(s) | 126, 520 |
| MalI | GATC | 3 cut(s) | 150, 205, 642 |
| MboI | GATC | 3 cut(s) | 148, 203, 640 |
| MboII | GAAGA | 6 cut(s) | 98, 162, 326, 334, 710, 1031 |
| MfeI | CAATTG | 1 cut(s) | 1014 |
| MhlI | GDGCHC | 3 cut(s) | 42, 570, 754 |
| MluCI | AATT | 9 cut(s) | 174, 188, 197, 260, 497, 722, 906, 967, 1014 |
| MlyI | GAGTC | 3 cut(s) | 105, 665, 905 |
| MmeI | TCCRAC | 1 cut(s) | 606 |
| MroXI | GAANNNNTTC | 2 cut(s) | 71, 717 |
| MslI | CAYNNNNRTG | 1 cut(s) | 983 |
| MspI | CCGG | 2 cut(s) | 107, 257 |
| MspR9I | CCNGG | 3 cut(s) | 32, 107, 233 |
| MunI | CAATTG | 1 cut(s) | 1014 |
| Mva1269I | GAATGC | 1 cut(s) | 881 |
| MvaI | CCWGG | 2 cut(s) | 32, 233 |
| MvnI | CGCG | 1 cut(s) | 770 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 161 |
| NciI | CCSGG | 1 cut(s) | 107 |
| NdeII | GATC | 3 cut(s) | 148, 203, 640 |
| NlaIII | CATG | 4 cut(s) | 547, 601, 630, 692 |
| NlaIV | GGNNCC | 1 cut(s) | 60 |
| PceI | AGGCCT | 1 cut(s) | 637 |
| PctI | GAATGC | 1 cut(s) | 881 |
| PdmI | GAANNNNTTC | 2 cut(s) | 71, 717 |
| PfeI | GAWTC | 3 cut(s) | 113, 473, 609 |
| PflFI | GACNNNGTC | 2 cut(s) | 272, 550 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 130 |
| PfoI | TCCNGGA | 2 cut(s) | 30, 105 |
| PkrI | GCNGC | 2 cut(s) | 166, 880 |
| PleI | GAGTC | 3 cut(s) | 104, 665, 904 |
| PpsI | GAGTC | 3 cut(s) | 104, 665, 904 |
| Psp124BI | GAGCTC | 1 cut(s) | 754 |
| Psp1406I | AACGTT | 1 cut(s) | 454 |
| Psp6I | CCWGG | 2 cut(s) | 30, 231 |
| PspGI | CCWGG | 2 cut(s) | 30, 231 |
| PspN4I | GGNNCC | 1 cut(s) | 60 |
| PspPI | GGNCC | 2 cut(s) | 274, 493 |
| PstI | CTGCAG | 1 cut(s) | 184 |
| PstNI | CAGNNNCTG | 1 cut(s) | 130 |
| PsyI | GACNNNGTC | 2 cut(s) | 272, 550 |
| RsaI | GTAC | 4 cut(s) | 329, 834, 902, 953 |
| RsaNI | GTAC | 4 cut(s) | 328, 833, 901, 952 |
| RseI | CAYNNNNRTG | 1 cut(s) | 983 |
| SacI | GAGCTC | 1 cut(s) | 754 |
| SatI | GCNGC | 2 cut(s) | 165, 879 |
| Sau3AI | GATC | 3 cut(s) | 148, 203, 640 |
| Sau96I | GGNCC | 2 cut(s) | 274, 493 |
| ScaI | AGTACT | 1 cut(s) | 329 |
| SchI | GAGTC | 3 cut(s) | 105, 665, 905 |
| ScrFI | CCNGG | 3 cut(s) | 32, 107, 233 |
| SduI | GDGCHC | 3 cut(s) | 42, 570, 754 |
| SfaNI | GCATC | 1 cut(s) | 8 |
| SfcI | CTRYAG | 1 cut(s) | 180 |
| SinI | GGWCC | 2 cut(s) | 274, 493 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 983 |
| SmlI | CTYRAG | 2 cut(s) | 331, 536 |
| SmoI | CTYRAG | 2 cut(s) | 331, 536 |
| Sse9I | AATT | 9 cut(s) | 174, 188, 197, 260, 497, 722, 906, 967, 1014 |
| SseBI | AGGCCT | 1 cut(s) | 637 |
| SsiI | CCGC | 4 cut(s) | 442, 484, 878, 893 |
| SspI | AATATT | 1 cut(s) | 71 |
| SstI | GAGCTC | 1 cut(s) | 754 |
| StuI | AGGCCT | 1 cut(s) | 637 |
| StyD4I | CCNGG | 3 cut(s) | 30, 105, 231 |
| TaaI | ACNGT | 4 cut(s) | 28, 294, 424, 532 |
| TaiI | ACGT | 4 cut(s) | 273, 351, 372, 457 |
| TaqI | TCGA | 2 cut(s) | 748, 938 |
| TasI | AATT | 9 cut(s) | 174, 188, 197, 260, 497, 722, 906, 967, 1014 |
| TatI | WGTACW | 3 cut(s) | 327, 832, 951 |
| TauI | GCSGC | 1 cut(s) | 881 |
| TfiI | GAWTC | 3 cut(s) | 113, 473, 609 |
| TseI | GCWGC | 1 cut(s) | 164 |
| TspDTI | ATGAA | 1 cut(s) | 201 |
| TspGWI | ACGGA | 3 cut(s) | 395, 521, 828 |
| Tth111I | GACNNNGTC | 2 cut(s) | 272, 550 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 130 |
| VpaK11BI | GGWCC | 2 cut(s) | 274, 493 |
| XapI | RAATTY | 3 cut(s) | 188, 197, 260 |
| XmnI | GAANNNNTTC | 2 cut(s) | 71, 717 |
| ZrmI | AGTACT | 1 cut(s) | 329 |