Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0001429

Physical Location & Seq

Genomic Coordinates Forward (+)

1412696 .. 1414606

Gene Structure 1911 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1019700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1050 bp

ATGGAGTTATTAGGATCTAAAACTGTCCAAGAGGTACTCATCCAAGGGGAACAGGTGCCAGATAAATATATCCGTAAAGTTGAAGATGGTGGAGTCCGAGTCCCAGTCCCAGATGCTTCTGCTGCCCAGTTGATGGATGTTCCAGTCATTGATCTTGCTCTTCTCGCAGCTTCTTCAATCACTTCAGATGAACTTGAGAAATTCAGATCAGCTCTTGCCACATGGGGTTGCTTCCAGGTAATAAACCACAGTATGTCGCCGGAATTCCTAGACGAGGTCCGAGAGATTACAAAACAGTTTTTTGCACTTCCAGTTGCAGATAAGAAGAAATACTTGAGACAAGTCAACGACATTCAAGGATATGGAAACGACATGGTTTTTTCAGAGCAACAAACACTAGATTGGTCTGATAGACTATACCTTTCTGTGTATCCACAAGACAACCGTAAGCTCAAATTTTGGCCCAAAAATCCGGACTCTTTTAGCCGGACTTTAGACCAATATGCCATGGAGTTACAAGTGGTAACAAAAACTATCCTCAAGGCCATGACAAGGTCATTGGATTTGGAGGACAATTGCTTTTCCGACCTATATGGTGAACAAGGGAAAATGGCTATCAGATTTAACTTTTATCCTCCATGTCCAAGGCCTGATCTCGTCCTTGGTCTCAAACCACACGCAGACGGAACACTAATCACCCTTGTCTTGCAAGATAAGGAAGTTGAAGGTCTTCAATTTCTTAAAGGCGATCACTGGTTTCGAGCTCCCATTGTTCCTGACGCGCTTCTCATGAATATCGGTGATCAAGTAGAGATATTGAGCAATGGAATATTCAAGAGTCCTGTACACAAGGTGGTGACAAATCCAGACAAGGAGAGGATATCTTTGGCGGCCTTCTGCATGCCAGAGTCGAACAAAGAGATTGAACCTTTTGAAAGGTTGGTCGATGAGTCAAGACCAAGATTGTACAAAAAGGTGACCAATTATGATGGCATCTATTTCGAATACTATCAGCAAGGGAGGAGACCAATTGAAGCAGCGAGATTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0010150 GO:0016491 GO:0016679 GO:0016682 GO:0032501 GO:0032502 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0055114 GO:0090693 GO:0099402

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

350

Amino Acids

39.94

Weight (kDa)

5.19

Isoelectric Point (pI)

43.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	47 - 157	7.1e-27	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	204 - 302	1.2e-30	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001429)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G49390 AT1G49390 AT5G20400 AT5G20550 AT5G54000 AT5G54000
fragaria_vesca	FvH4_1g02121 FvH4_1g02150 FvH4_1g02151 FvH4_1g02152 FvH4_1g02160 FvH4_1g02160 FvH4_1g02160 FvH4_1g02160
malus_domestica	MD02G1019700.v1.1 MD02G1020000.v1.1 MD02G1020100.v1.1 MD14G1108700.v1.1 MD14G1108800.v1.1
prunus_persica	Prupe.4G248100_v2.0.a1 Prupe.4G248200_v2.0.a1 Prupe.4G248300_v2.0.a1 Prupe.4G248400_v2.0.a1 Prupe.4G248600_v2.0.a1
pyrus_communis	pycom02g01750 pycom02g01760
rosa_chinensis	RchiOBHm_Chr2g0087201 RchiOBHm_Chr2g0087211 RchiOBHm_Chr2g0087231 RchiOBHm_Chr2g0087261
rosa_laevigata	RLG00000015844 RLG00000015846 RLG00000015848
rosa_multiflora	Rmu_co8425197.1_g000001 Rmu_co8475215.1_g000001 Rmu_sc0003367.1_g000009 Rmu_sc0003367.1_g000015 Rmu_sc0018794.1_g000005
rosa_roxburghii	Rroxscaffold_2G00153770 Rroxscaffold_2G00153780 Rroxscaffold_2G00153810
rosa_rugosa	Rorug01G0473300 Rorug01G0473600
rosa_samantha	Rh2AG026600 Rh2AG026900 Rh2AG027100 Rh2BG026400 Rh2DG026700 Rh2DG026900 Rh2DG027100
rosa_wichuraiana	Rw2G002120 Rw2G002130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	55
AccB7I	CCANNNNNTGG	1 cut(s)	133
AccII	CGCG	1 cut(s)	782
AccIII	TCCGGA	1 cut(s)	472
AciI	CCGC	1 cut(s)	890
AclWI	GGATC	1 cut(s)	22
AcsI	RAATTY	3 cut(s)	200, 263, 455
AcuI	CTGAAG	1 cut(s)	168
AdeI	CACNNNGTG	1 cut(s)	853
AfaI	GTAC	3 cut(s)	36, 846, 968
AfiI	CCNNNNNNNGG	3 cut(s)	133, 274, 552
AgsI	TTSAA	9 cut(s)	83, 177, 356, 725, 734, 835, 926, 935, 1034
AjnI	CCWGG	1 cut(s)	234
AluBI	AGCT	4 cut(s)	170, 212, 451, 764
AluI	AGCT	4 cut(s)	170, 212, 451, 764
Alw21I	GWGCWC	1 cut(s)	766
Alw26I	GTCTC	3 cut(s)	331, 671, 1018
AlwI	GGATC	1 cut(s)	22
Aor13HI	TCCGGA	1 cut(s)	472
AoxI	GGCC	4 cut(s)	461, 543, 647, 891
ApeKI	GCWGC	3 cut(s)	122, 167, 1037
ApoI	RAATTY	3 cut(s)	200, 263, 455
Asp700I	GAANNNNTTC	1 cut(s)	729
AspLEI	GCGC	1 cut(s)	784
AspS9I	GGNCC	2 cut(s)	277, 462
AsuHPI	GGTGA	5 cut(s)	608, 688, 812, 868, 988
AsuII	TTCGAA	1 cut(s)	1002
AvaII	GGWCC	1 cut(s)	277
BanI	GGYRCC	1 cut(s)	55
BanII	GRGCYC	1 cut(s)	766
BbsI	GAAGAC	1 cut(s)	722
Bbv12I	GWGCWC	1 cut(s)	766
BbvI	GCAGC	2 cut(s)	109, 179
BccI	CCATC	3 cut(s)	80, 127, 983
BcgI	CGANNNNNNTGC	2 cut(s)	982, 1016
BciT130I	CCWGG	1 cut(s)	236
BciVI	GTATCC	1 cut(s)	441
BclI	TGATCA	1 cut(s)	802
BcoDI	GTCTC	3 cut(s)	331, 671, 1018
BfaI	CTAG	2 cut(s)	269, 398
BfuI	GTATCC	1 cut(s)	441
BisI	GCNGC	4 cut(s)	123, 168, 891, 1038
BlsI	GCNGC	4 cut(s)	124, 169, 892, 1039
Bme1390I	CCNGG	1 cut(s)	236
Bme18I	GGWCC	1 cut(s)	277
BmgT120I	GGNCC	2 cut(s)	277, 462
BmiI	GGNNCC	1 cut(s)	57
BmrFI	CCNGG	1 cut(s)	236
BmrI	ACTGGG	2 cut(s)	98, 121
BmsI	GCATC	2 cut(s)	103, 1002
BmuI	ACTGGG	2 cut(s)	98, 121
BpiI	GAAGAC	1 cut(s)	722
Bpu14I	TTCGAA	1 cut(s)	1002
BpuEI	CTTGAG	3 cut(s)	215, 355, 524
BsaI	GGTCTC	2 cut(s)	671, 1018
BsaJI	CCNNGG	4 cut(s)	43, 507, 644, 661
BsaWI	WCCGGW	1 cut(s)	472
BsaXI	ACNNNNNCTCC	2 cut(s)	84, 114
Bsc4I	CCNNNNNNNGG	3 cut(s)	133, 274, 552
Bse1I	ACTGG	5 cut(s)	104, 127, 143, 311, 758
Bse3DI	GCAATG	1 cut(s)	829
BseAI	TCCGGA	1 cut(s)	472
BseBI	CCWGG	1 cut(s)	236
BseDI	CCNNGG	4 cut(s)	43, 507, 644, 661
BseGI	GGATG	2 cut(s)	39, 142
BseLI	CCNNNNNNNGG	3 cut(s)	133, 274, 552
BseMI	GCAATG	1 cut(s)	829
BseNI	ACTGG	5 cut(s)	104, 127, 143, 311, 758
BseRI	GAGGAG	1 cut(s)	1036
BseXI	GCAGC	2 cut(s)	109, 179
Bsh1236I	CGCG	1 cut(s)	782
BshFI	GGCC	4 cut(s)	463, 545, 649, 893
BshNI	GGYRCC	1 cut(s)	55
BsiHKAI	GWGCWC	1 cut(s)	766
BsiSI	CCGG	3 cut(s)	260, 473, 487
BslFI	GGGAC	2 cut(s)	86, 92
BslI	CCNNNNNNNGG	3 cut(s)	133, 274, 552
BsmAI	GTCTC	3 cut(s)	331, 671, 1018
BsmFI	GGGAC	2 cut(s)	86, 92
BsnI	GGCC	4 cut(s)	463, 545, 649, 893
Bso31I	GGTCTC	2 cut(s)	671, 1018
Bsp119I	TTCGAA	1 cut(s)	1002
Bsp1286I	GDGCHC	1 cut(s)	766
Bsp13I	TCCGGA	1 cut(s)	472
Bsp1407I	TGTACA	2 cut(s)	844, 966
Bsp143I	GATC	6 cut(s)	14, 151, 206, 652, 748, 802
Bsp19I	CCATGG	1 cut(s)	507
BspACI	CCGC	1 cut(s)	890
BspANI	GGCC	4 cut(s)	463, 545, 649, 893
BspEI	TCCGGA	1 cut(s)	472
BspFNI	CGCG	1 cut(s)	782
BspHI	TCATGA	1 cut(s)	789
BspLI	GGNNCC	1 cut(s)	57
BspPI	GGATC	1 cut(s)	22
BspQI	GCTCTTC	1 cut(s)	165
BspT104I	TTCGAA	1 cut(s)	1002
BspT107I	GGYRCC	1 cut(s)	55
BspTNI	GGTCTC	2 cut(s)	671, 1018
BsrDI	GCAATG	1 cut(s)	829
BsrGI	TGTACA	2 cut(s)	844, 966
BsrI	ACTGG	5 cut(s)	104, 127, 143, 311, 758
BssECI	CCNNGG	4 cut(s)	43, 507, 644, 661
BssMI	GATC	6 cut(s)	14, 151, 206, 652, 748, 802
BssT1I	CCWWGG	4 cut(s)	43, 507, 644, 661
Bst2UI	CCWGG	1 cut(s)	236
Bst4CI	ACNGT	4 cut(s)	25, 251, 297, 446
Bst6I	CTCTTC	1 cut(s)	165
BstAUI	TGTACA	2 cut(s)	844, 966
BstBI	TTCGAA	1 cut(s)	1002
BstC8I	GCNNGC	1 cut(s)	902
BstDSI	CCRYGG	1 cut(s)	507
BstEII	GGTNACC	1 cut(s)	976
BstENI	CCTNNNNNAGG	1 cut(s)	272
BstF5I	GGATG	2 cut(s)	39, 142
BstFNI	CGCG	1 cut(s)	782
BstHHI	GCGC	1 cut(s)	784
BstKTI	GATC	6 cut(s)	17, 154, 209, 655, 751, 805
BstMAI	GTCTC	3 cut(s)	331, 671, 1018
BstMBI	GATC	6 cut(s)	14, 151, 206, 652, 748, 802
BstMWI	GCNNNNNNNGC	2 cut(s)	122, 164
BstNI	CCWGG	1 cut(s)	236
BstNSI	RCATGY	1 cut(s)	904
BstPI	GGTNACC	1 cut(s)	976
BstSCI	CCNGG	1 cut(s)	234
BstUI	CGCG	1 cut(s)	782
BstV1I	GCAGC	2 cut(s)	109, 179
BstV2I	GAAGAC	1 cut(s)	722
BstX2I	RGATCY	1 cut(s)	14
BstYI	RGATCY	1 cut(s)	14
BsuI	GTATCC	1 cut(s)	441
BsuRI	GGCC	4 cut(s)	463, 545, 649, 893
BtgI	CCRYGG	1 cut(s)	507
BtsCI	GGATG	2 cut(s)	39, 142
BtsIMutI	CAGTG	1 cut(s)	751
Cac8I	GCNNGC	1 cut(s)	902
CciI	TCATGA	1 cut(s)	789
CfoI	GCGC	1 cut(s)	784
Cfr13I	GGNCC	2 cut(s)	277, 462
CseI	GACGC	1 cut(s)	788
Csp6I	GTAC	3 cut(s)	35, 845, 967
CviAII	CATG	7 cut(s)	222, 373, 508, 547, 639, 790, 901
CviQI	GTAC	3 cut(s)	35, 845, 967
DpnI	GATC	6 cut(s)	16, 153, 208, 654, 750, 804
DpnII	GATC	6 cut(s)	14, 151, 206, 652, 748, 802
DraIII	CACNNNGTG	1 cut(s)	853
Eam1104I	CTCTTC	1 cut(s)	165
EarI	CTCTTC	1 cut(s)	165
Ecl136II	GAGCTC	1 cut(s)	764
Eco130I	CCWWGG	4 cut(s)	43, 507, 644, 661
Eco147I	AGGCCT	1 cut(s)	649
Eco24I	GRGCYC	1 cut(s)	766
Eco31I	GGTCTC	2 cut(s)	671, 1018
Eco32I	GATATC	1 cut(s)	882
Eco47I	GGWCC	1 cut(s)	277
Eco53kI	GAGCTC	1 cut(s)	764
Eco57I	CTGAAG	1 cut(s)	168
Eco91I	GGTNACC	1 cut(s)	976
EcoICRI	GAGCTC	1 cut(s)	764
EcoNI	CCTNNNNNAGG	1 cut(s)	272
EcoO65I	GGTNACC	1 cut(s)	976
EcoRI	GAATTC	1 cut(s)	263
EcoRII	CCWGG	1 cut(s)	234
EcoRV	GATATC	1 cut(s)	882
EcoT14I	CCWWGG	4 cut(s)	43, 507, 644, 661
EcoT38I	GRGCYC	1 cut(s)	766
ErhI	CCWWGG	4 cut(s)	43, 507, 644, 661
FaeI	CATG	7 cut(s)	225, 376, 511, 550, 642, 793, 904
FalI	AAGNNNNNCTT	2 cut(s)	317, 349
FaqI	GGGAC	2 cut(s)	86, 92
FatI	CATG	7 cut(s)	221, 372, 507, 546, 638, 789, 900
FbaI	TGATCA	1 cut(s)	802
Fnu4HI	GCNGC	4 cut(s)	123, 168, 891, 1038
FokI	GGATG	2 cut(s)	26, 149
FriOI	GRGCYC	1 cut(s)	766
Fsp4HI	GCNGC	4 cut(s)	123, 168, 891, 1038
FspBI	CTAG	2 cut(s)	269, 398
GlaI	GCGC	1 cut(s)	783
GluI	GCNGC	4 cut(s)	123, 168, 891, 1038
HaeIII	GGCC	4 cut(s)	463, 545, 649, 893
HapII	CCGG	3 cut(s)	260, 473, 487
HgaI	GACGC	1 cut(s)	788
HhaI	GCGC	1 cut(s)	784
Hin1II	CATG	7 cut(s)	225, 376, 511, 550, 642, 793, 904
Hin6I	GCGC	1 cut(s)	782
HinP1I	GCGC	1 cut(s)	782
HincII	GTYRAC	1 cut(s)	346
HindII	GTYRAC	1 cut(s)	346
HinfI	GANTC	6 cut(s)	93, 99, 476, 838, 908, 950
HpaII	CCGG	3 cut(s)	260, 473, 487
HphI	GGTGA	5 cut(s)	608, 688, 812, 868, 988
Hpy166II	GTNNAC	3 cut(s)	346, 599, 847
Hpy188I	TCNGA	8 cut(s)	98, 187, 206, 281, 385, 409, 586, 620
Hpy188III	TCNNGA	6 cut(s)	473, 776, 790, 835, 866, 954
Hpy8I	GTNNAC	3 cut(s)	346, 599, 847
HpyAV	CCTTC	2 cut(s)	719, 904
HpyCH4III	ACNGT	4 cut(s)	25, 251, 297, 446
HpyCH4V	TGCA	4 cut(s)	305, 317, 709, 900
HpyF10VI	GCNNNNNNNGC	2 cut(s)	122, 164
Hsp92II	CATG	7 cut(s)	225, 376, 511, 550, 642, 793, 904
HspAI	GCGC	1 cut(s)	782
Kpn2I	TCCGGA	1 cut(s)	472
Ksp22I	TGATCA	1 cut(s)	802
Kzo9I	GATC	6 cut(s)	14, 151, 206, 652, 748, 802
LguI	GCTCTTC	1 cut(s)	165
LmnI	GCTCC	1 cut(s)	769
Lsp1109I	GCAGC	2 cut(s)	109, 179
LweI	GCATC	2 cut(s)	103, 1002
MaeI	CTAG	2 cut(s)	269, 398
MaeIII	GTNAC	4 cut(s)	513, 523, 856, 976
MalI	GATC	6 cut(s)	16, 153, 208, 654, 750, 804
MboI	GATC	6 cut(s)	14, 151, 206, 652, 748, 802
MboII	GAAGA	5 cut(s)	95, 152, 165, 337, 722
MfeI	CAATTG	2 cut(s)	574, 1029
MflI	RGATCY	1 cut(s)	14
MhlI	GDGCHC	1 cut(s)	766
MluCI	AATT	7 cut(s)	200, 263, 455, 574, 734, 982, 1029
MlyI	GAGTC	6 cut(s)	102, 108, 470, 847, 917, 959
MmeI	TCCRAC	1 cut(s)	609
MnlI	CCTC	7 cut(s)	25, 268, 548, 562, 645, 870, 1014
MroI	TCCGGA	1 cut(s)	472
MroXI	GAANNNNTTC	1 cut(s)	729
MseI	TTAA	2 cut(s)	624, 741
MspI	CCGG	3 cut(s)	260, 473, 487
MspR9I	CCNGG	1 cut(s)	236
MunI	CAATTG	2 cut(s)	574, 1029
MvaI	CCWGG	1 cut(s)	236
MvnI	CGCG	1 cut(s)	782
MwoI	GCNNNNNNNGC	2 cut(s)	122, 164
NcoI	CCATGG	1 cut(s)	507
NdeII	GATC	6 cut(s)	14, 151, 206, 652, 748, 802
NlaIII	CATG	7 cut(s)	225, 376, 511, 550, 642, 793, 904
NlaIV	GGNNCC	1 cut(s)	57
NmuCI	GTSAC	2 cut(s)	856, 976
NspI	RCATGY	1 cut(s)	904
NspV	TTCGAA	1 cut(s)	1002
PaeI	GCATGC	1 cut(s)	904
PagI	TCATGA	1 cut(s)	789
PceI	AGGCCT	1 cut(s)	649
PciSI	GCTCTTC	1 cut(s)	165
PdmI	GAANNNNTTC	1 cut(s)	729
PflFI	GACNNNGTC	2 cut(s)	275, 553
PflMI	CCANNNNNTGG	1 cut(s)	133
PkrI	GCNGC	4 cut(s)	124, 169, 892, 1039
PleI	GAGTC	6 cut(s)	101, 107, 470, 846, 916, 958
PpsI	GAGTC	6 cut(s)	101, 107, 470, 846, 916, 958
Psp124BI	GAGCTC	1 cut(s)	766
Psp6I	CCWGG	1 cut(s)	234
PspEI	GGTNACC	1 cut(s)	976
PspGI	CCWGG	1 cut(s)	234
PspN4I	GGNNCC	1 cut(s)	57
PspPI	GGNCC	2 cut(s)	277, 462
PsuI	RGATCY	1 cut(s)	14
PsyI	GACNNNGTC	2 cut(s)	275, 553
RsaI	GTAC	3 cut(s)	36, 846, 968
RsaNI	GTAC	3 cut(s)	35, 845, 967
SacI	GAGCTC	1 cut(s)	766
SapI	GCTCTTC	1 cut(s)	165
SaqAI	TTAA	2 cut(s)	624, 741
SatI	GCNGC	4 cut(s)	123, 168, 891, 1038
Sau3AI	GATC	6 cut(s)	14, 151, 206, 652, 748, 802
Sau96I	GGNCC	2 cut(s)	277, 462
SchI	GAGTC	6 cut(s)	102, 108, 470, 847, 917, 959
ScrFI	CCNGG	1 cut(s)	236
SduI	GDGCHC	1 cut(s)	766
SfaNI	GCATC	2 cut(s)	103, 1002
SfuI	TTCGAA	1 cut(s)	1002
SinI	GGWCC	1 cut(s)	277
SmlI	CTYRAG	3 cut(s)	194, 334, 539
SmoI	CTYRAG	3 cut(s)	194, 334, 539
SphI	GCATGC	1 cut(s)	904
Sse9I	AATT	7 cut(s)	200, 263, 455, 574, 734, 982, 1029
SseBI	AGGCCT	1 cut(s)	649
SsiI	CCGC	1 cut(s)	890
SspI	AATATT	1 cut(s)	831
SspMI	CTAG	2 cut(s)	269, 398
SstI	GAGCTC	1 cut(s)	766
StuI	AGGCCT	1 cut(s)	649
StyD4I	CCNGG	1 cut(s)	234
StyI	CCWWGG	4 cut(s)	43, 507, 644, 661
TaaI	ACNGT	4 cut(s)	25, 251, 297, 446
TaqI	TCGA	4 cut(s)	760, 911, 945, 1002
TasI	AATT	7 cut(s)	200, 263, 455, 574, 734, 982, 1029
TatI	WGTACW	2 cut(s)	844, 966
TauI	GCSGC	1 cut(s)	893
Tru1I	TTAA	2 cut(s)	624, 741
Tru9I	TTAA	2 cut(s)	624, 741
TscAI	CASTG	1 cut(s)	758
TseFI	GTSAC	2 cut(s)	856, 976
TseI	GCWGC	3 cut(s)	122, 167, 1037
Tsp45I	GTSAC	2 cut(s)	856, 976
TspDTI	ATGAA	2 cut(s)	204, 806
TspGWI	ACGGA	2 cut(s)	62, 699
TspRI	CASTG	1 cut(s)	758
Tth111I	GACNNNGTC	2 cut(s)	275, 553
Van91I	CCANNNNNTGG	1 cut(s)	133
VpaK11BI	GGWCC	1 cut(s)	277
XagI	CCTNNNNNAGG	1 cut(s)	272
XapI	RAATTY	3 cut(s)	200, 263, 455
XceI	RCATGY	1 cut(s)	904
XmnI	GAANNNNTTC	1 cut(s)	729
XspI	CTAG	2 cut(s)	269, 398

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.