FvH4_1g02121

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001429
Physical Location & Seq
Forward (+)
1141433 .. 1143245
1813 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g02121.t1

Sequence Viewer

Length: 1065 bp
ATGGCTAAATCTTCCGCGCCTGCCCAGTTCGAGTTATTAGCAACCAAAACTGTCCAAGAGCAACTCATAGAAGGAGAAGTACTGGTACCCCCACCGAAATATATTCTCAAAGATGGAGTTCCACTTCCAGATGCTTCTGTTGAGTTCATGGATGTTCCAGTTATTGATCTTGGCCTCCTCACACCATCCTCAATCAGTATGGAAGAATTTGAAAAACTCAGATCAGCTTTAAACAAATGGGGTTGCTTTCAGGTAGTTAACCATGGAATGACTCCTGAATTCCTTGACCAAGTCCGTGAAATCACGAAACAGTTTTTTGCAATGCCAGCGGAAGAGAAGAAGAAATATTTGAGGCCAGTCGACAATATTGAAGGATATGGGAATGACATGGTATTTTCAGAGCAGCAAACACTTGATTGGACCGACAGACTATACCTTACAGTATATCCACCAGACAAGCACAAGCTAAAGTTTTGGCCTGAAAATCCCAAATCTTTCAGGGATACTCTAGACCAATATACCTGTAAGTTACAGGTGATAACAGAAACTGTCCTTAAGGGCATGGCGCGGTCATTGAATTTGGAGGAAGATTGCTTTCTGAAAAAGTATGGAGAACAAAGGAAAATGGATGCTAGGTTCAACTTCTATCCTCCATGTTCTAGGCCTGATCGCGTACTCGGTACCAAGCCACATGCAGATGGAACATTAATAACCCTTCTGTTGCAAGATAAAGAAGTGGAAGGTCTTCAATTTATGAAAGATGACCGATGGTATAGGGCTCCCATTGTTCCGGAGGCGCTTCTCATTAATGTTGGTGATCAAGCTGAGATATTGAGCAATGGACTGTTCAAGAGTCCGGTGCACAAGGTTGTAACAAATGCAGAGAAGGAGAGGATATCCTTGGCTGTGTTCTGTGTCCCGGATTCAGAGACAGAGATTGAACCCTTTGAAAGCCTCATCAATGAGTCAAGGCCAAGGTTGTACAAAAAGCTGAAGAACTACGTTGGCATCTATTTCGAGTACTACCAGCAGGGAAGAAGACCAATTGAAGCAGCAATAATATAA

Protein Analysis

355

Amino Acids

40.77

Weight (kDa)

5.33

Isoelectric Point (pI)

49.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 52 - 162 3.3e-29 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 212 - 307 3.6e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 85, 680
AccB1I GGYRCC 2 cut(s) 85, 680
AccI GTMKAC 1 cut(s) 360
AccII CGCG 3 cut(s) 17, 568, 672
AccIII TCCGGA 1 cut(s) 790
AciI CCGC 3 cut(s) 15, 329, 568
AcsI RAATTY 3 cut(s) 206, 278, 577
AcuI CTGAAG 1 cut(s) 1013
AfaI GTAC 6 cut(s) 81, 87, 675, 682, 983, 1022
AflII CTTAAG 1 cut(s) 554
AgsI TTSAA 9 cut(s) 212, 371, 577, 640, 749, 850, 941, 950, 1049
AloI GAACNNNNNNTCC 2 cut(s) 620, 652
AluBI AGCT 4 cut(s) 227, 466, 824, 991
AluI AGCT 4 cut(s) 227, 466, 824, 991
Alw21I GWGCWC 1 cut(s) 864
Alw26I GTCTC 1 cut(s) 923
Alw44I GTGCAC 1 cut(s) 860
AlwNI CAGNNNCTG 1 cut(s) 548
Aor13HI TCCGGA 1 cut(s) 790
AoxI GGCC 5 cut(s) 172, 353, 476, 662, 971
ApaLI GTGCAC 1 cut(s) 860
ApeKI GCWGC 2 cut(s) 403, 1052
ApoI RAATTY 3 cut(s) 206, 278, 577
AseI ATTAAT 2 cut(s) 707, 807
Asp700I GAANNNNTTC 1 cut(s) 744
Asp718I GGTACC 2 cut(s) 85, 680
AspLEI GCGC 3 cut(s) 19, 568, 799
AspS9I GGNCC 1 cut(s) 420
AsuC2I CCSGG 1 cut(s) 920
AsuHPI GGTGA 2 cut(s) 547, 827
AvaII GGWCC 1 cut(s) 420
BaeGI GKGCMC 1 cut(s) 864
BanI GGYRCC 2 cut(s) 85, 680
BanII GRGCYC 1 cut(s) 781
BarI GAAGNNNNNNTAC 4 cut(s) 63, 69, 95, 101
BbsI GAAGAC 2 cut(s) 737, 1045
Bbv12I GWGCWC 1 cut(s) 864
BbvI GCAGC 1 cut(s) 415
BccI CCATC 4 cut(s) 107, 193, 692, 762
BcgI CGANNNNNNTGC 2 cut(s) 997, 1031
BciVI GTATCC 1 cut(s) 496
BclI TGATCA 1 cut(s) 817
BcnI CCSGG 1 cut(s) 920
BcoDI GTCTC 1 cut(s) 923
BfaI CTAG 3 cut(s) 509, 633, 660
BfoI RGCGCY 1 cut(s) 800
BfrI CTTAAG 1 cut(s) 554
BfuI GTATCC 1 cut(s) 496
BisI GCNGC 2 cut(s) 404, 1053
BlsI GCNGC 2 cut(s) 405, 1054
BmcAI AGTACT 2 cut(s) 81, 1022
Bme1390I CCNGG 1 cut(s) 920
Bme18I GGWCC 1 cut(s) 420
BmgT120I GGNCC 1 cut(s) 420
BmiI GGNNCC 3 cut(s) 87, 682, 780
BmrFI CCNGG 1 cut(s) 920
BmrI ACTGGG 1 cut(s) 19
BmsI GCATC 3 cut(s) 121, 619, 1017
BmuI ACTGGG 1 cut(s) 19
BpiI GAAGAC 2 cut(s) 737, 1045
BpuMI CCSGG 1 cut(s) 920
BsaJI CCNNGG 3 cut(s) 262, 900, 974
BsaWI WCCGGW 2 cut(s) 790, 856
Bse1I ACTGG 4 cut(s) 25, 87, 158, 356
Bse3DI GCAATG 2 cut(s) 327, 844
BseAI TCCGGA 1 cut(s) 790
BseDI CCNNGG 3 cut(s) 262, 900, 974
BseGI GGATG 3 cut(s) 157, 185, 634
BseMI GCAATG 2 cut(s) 327, 844
BseMII CTCAG 2 cut(s) 232, 816
BseNI ACTGG 4 cut(s) 25, 87, 158, 356
BseRI GAGGAG 1 cut(s) 167
BseSI GKGCMC 1 cut(s) 864
BseXI GCAGC 1 cut(s) 415
Bsh1236I CGCG 3 cut(s) 17, 568, 672
BshFI GGCC 5 cut(s) 174, 355, 478, 664, 973
BshNI GGYRCC 2 cut(s) 85, 680
BsiHKAI GWGCWC 1 cut(s) 864
BsiSI CCGG 3 cut(s) 791, 857, 920
BslFI GGGAC 1 cut(s) 902
BsmAI GTCTC 1 cut(s) 923
BsmFI GGGAC 1 cut(s) 902
BsnI GGCC 5 cut(s) 174, 355, 478, 664, 973
Bsp1286I GDGCHC 2 cut(s) 781, 864
Bsp13I TCCGGA 1 cut(s) 790
Bsp1407I TGTACA 1 cut(s) 981
Bsp143I GATC 4 cut(s) 166, 221, 667, 817
Bsp19I CCATGG 1 cut(s) 262
BspACI CCGC 3 cut(s) 15, 329, 568
BspANI GGCC 5 cut(s) 174, 355, 478, 664, 973
BspCNI CTCAG 2 cut(s) 231, 817
BspEI TCCGGA 1 cut(s) 790
BspFNI CGCG 3 cut(s) 17, 568, 672
BspLI GGNNCC 3 cut(s) 87, 682, 780
BspT107I GGYRCC 2 cut(s) 85, 680
BspTI CTTAAG 1 cut(s) 554
BsrDI GCAATG 2 cut(s) 327, 844
BsrGI TGTACA 1 cut(s) 981
BsrI ACTGG 4 cut(s) 25, 87, 158, 356
BssECI CCNNGG 3 cut(s) 262, 900, 974
BssMI GATC 4 cut(s) 166, 221, 667, 817
BssT1I CCWWGG 3 cut(s) 262, 900, 974
Bst4CI ACNGT 5 cut(s) 52, 312, 442, 550, 846
Bst6I CTCTTC 1 cut(s) 327
BstAFI CTTAAG 1 cut(s) 554
BstAUI TGTACA 1 cut(s) 981
BstC8I GCNNGC 2 cut(s) 21, 327
BstDEI CTNAG 2 cut(s) 218, 825
BstDSI CCRYGG 1 cut(s) 262
BstF5I GGATG 3 cut(s) 157, 185, 634
BstFNI CGCG 3 cut(s) 17, 568, 672
BstH2I RGCGCY 1 cut(s) 800
BstHHI GCGC 3 cut(s) 19, 568, 799
BstKTI GATC 4 cut(s) 169, 224, 670, 820
BstMAI GTCTC 1 cut(s) 923
BstMBI GATC 4 cut(s) 166, 221, 667, 817
BstMWI GCNNNNNNNGC 1 cut(s) 326
BstNSI RCATGY 1 cut(s) 695
BstSCI CCNGG 1 cut(s) 918
BstSLI GKGCMC 1 cut(s) 864
BstUI CGCG 3 cut(s) 17, 568, 672
BstV1I GCAGC 1 cut(s) 415
BstV2I GAAGAC 2 cut(s) 737, 1045
BsuI GTATCC 1 cut(s) 496
BsuRI GGCC 5 cut(s) 174, 355, 478, 664, 973
BtgI CCRYGG 1 cut(s) 262
BtsCI GGATG 3 cut(s) 157, 185, 634
Cac8I GCNNGC 2 cut(s) 21, 327
CaiI CAGNNNCTG 1 cut(s) 548
CfoI GCGC 3 cut(s) 19, 568, 799
Cfr13I GGNCC 1 cut(s) 420
Csp6I GTAC 6 cut(s) 80, 86, 674, 681, 982, 1021
CviAII CATG 6 cut(s) 148, 263, 388, 562, 654, 692
CviQI GTAC 6 cut(s) 80, 86, 674, 681, 982, 1021
DdeI CTNAG 2 cut(s) 218, 825
DpnI GATC 4 cut(s) 168, 223, 669, 819
DpnII GATC 4 cut(s) 166, 221, 667, 817
DraI TTTAAA 1 cut(s) 231
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
Eco130I CCWWGG 3 cut(s) 262, 900, 974
Eco147I AGGCCT 1 cut(s) 664
Eco24I GRGCYC 1 cut(s) 781
Eco32I GATATC 1 cut(s) 897
Eco47I GGWCC 1 cut(s) 420
Eco57I CTGAAG 1 cut(s) 1013
EcoRI GAATTC 1 cut(s) 278
EcoRV GATATC 1 cut(s) 897
EcoT14I CCWWGG 3 cut(s) 262, 900, 974
EcoT38I GRGCYC 1 cut(s) 781
ErhI CCWWGG 3 cut(s) 262, 900, 974
FaeI CATG 6 cut(s) 151, 266, 391, 565, 657, 695
FaqI GGGAC 1 cut(s) 902
FatI CATG 6 cut(s) 147, 262, 387, 561, 653, 691
FbaI TGATCA 1 cut(s) 817
FblI GTMKAC 1 cut(s) 360
Fnu4HI GCNGC 2 cut(s) 404, 1053
FokI GGATG 3 cut(s) 164, 172, 641
FriOI GRGCYC 1 cut(s) 781
Fsp4HI GCNGC 2 cut(s) 404, 1053
FspBI CTAG 3 cut(s) 509, 633, 660
GlaI GCGC 3 cut(s) 18, 567, 798
GluI GCNGC 2 cut(s) 404, 1053
HaeII RGCGCY 1 cut(s) 800
HaeIII GGCC 5 cut(s) 174, 355, 478, 664, 973
HapII CCGG 3 cut(s) 791, 857, 920
HhaI GCGC 3 cut(s) 19, 568, 799
Hin1II CATG 6 cut(s) 151, 266, 391, 565, 657, 695
Hin6I GCGC 3 cut(s) 17, 566, 797
HinP1I GCGC 3 cut(s) 17, 566, 797
HincII GTYRAC 2 cut(s) 259, 361
HindII GTYRAC 2 cut(s) 259, 361
HinfI GANTC 4 cut(s) 271, 853, 923, 965
HpaI GTTAAC 1 cut(s) 259
HpaII CCGG 3 cut(s) 791, 857, 920
HphI GGTGA 2 cut(s) 547, 827
Hpy166II GTNNAC 3 cut(s) 259, 361, 862
Hpy188I TCNGA 4 cut(s) 221, 400, 600, 928
Hpy188III TCNNGA 6 cut(s) 128, 275, 304, 509, 791, 850
Hpy8I GTNNAC 3 cut(s) 259, 361, 862
HpyAV CCTTC 5 cut(s) 65, 365, 725, 734, 880
HpyCH4III ACNGT 5 cut(s) 52, 312, 442, 550, 846
HpyCH4IV ACGT 1 cut(s) 1002
HpyCH4V TGCA 5 cut(s) 320, 695, 724, 862, 881
HpyF10VI GCNNNNNNNGC 1 cut(s) 326
HpyF3I CTNAG 2 cut(s) 218, 825
HpySE526I ACGT 1 cut(s) 1002
Hsp92II CATG 6 cut(s) 151, 266, 391, 565, 657, 695
HspAI GCGC 3 cut(s) 17, 566, 797
Kpn2I TCCGGA 1 cut(s) 790
KpnI GGTACC 2 cut(s) 89, 684
Ksp22I TGATCA 1 cut(s) 817
KspAI GTTAAC 1 cut(s) 259
Kzo9I GATC 4 cut(s) 166, 221, 667, 817
LmnI GCTCC 1 cut(s) 784
Lsp1109I GCAGC 1 cut(s) 415
LweI GCATC 3 cut(s) 121, 619, 1017
MaeI CTAG 3 cut(s) 509, 633, 660
MaeII ACGT 1 cut(s) 1002
MaeIII GTNAC 2 cut(s) 528, 871
MalI GATC 4 cut(s) 168, 223, 669, 819
MboI GATC 4 cut(s) 166, 221, 667, 817
MfeI CAATTG 1 cut(s) 1044
MhlI GDGCHC 2 cut(s) 781, 864
MluCI AATT 5 cut(s) 206, 278, 577, 749, 1044
MlyI GAGTC 3 cut(s) 265, 862, 974
MnlI CCTC 9 cut(s) 185, 188, 199, 345, 577, 660, 787, 885, 965
MroI TCCGGA 1 cut(s) 790
MroXI GAANNNNTTC 1 cut(s) 744
MseI TTAA 5 cut(s) 230, 258, 555, 707, 807
MslI CAYNNNNRTG 1 cut(s) 696
MspA1I CMGCKG 1 cut(s) 329
MspCI CTTAAG 1 cut(s) 554
MspI CCGG 3 cut(s) 791, 857, 920
MspR9I CCNGG 1 cut(s) 920
MunI CAATTG 1 cut(s) 1044
MvnI CGCG 3 cut(s) 17, 568, 672
MwoI GCNNNNNNNGC 1 cut(s) 326
NciI CCSGG 1 cut(s) 920
NcoI CCATGG 1 cut(s) 262
NdeII GATC 4 cut(s) 166, 221, 667, 817
NlaIII CATG 6 cut(s) 151, 266, 391, 565, 657, 695
NlaIV GGNNCC 3 cut(s) 87, 682, 780
NspI RCATGY 1 cut(s) 695
PceI AGGCCT 1 cut(s) 664
PdmI GAANNNNTTC 1 cut(s) 744
PfeI GAWTC 1 cut(s) 923
PflFI GACNNNGTC 1 cut(s) 290
PfoI TCCNGGA 1 cut(s) 918
PkrI GCNGC 2 cut(s) 405, 1054
PleI GAGTC 3 cut(s) 265, 861, 973
PpsI GAGTC 3 cut(s) 265, 861, 973
PshBI ATTAAT 2 cut(s) 707, 807
PspN4I GGNNCC 3 cut(s) 87, 682, 780
PspPI GGNCC 1 cut(s) 420
PstNI CAGNNNCTG 1 cut(s) 548
PsyI GACNNNGTC 1 cut(s) 290
RsaI GTAC 6 cut(s) 81, 87, 675, 682, 983, 1022
RsaNI GTAC 6 cut(s) 80, 86, 674, 681, 982, 1021
RseI CAYNNNNRTG 1 cut(s) 696
SalI GTCGAC 1 cut(s) 359
SaqAI TTAA 5 cut(s) 230, 258, 555, 707, 807
SatI GCNGC 2 cut(s) 404, 1053
Sau3AI GATC 4 cut(s) 166, 221, 667, 817
Sau96I GGNCC 1 cut(s) 420
ScaI AGTACT 2 cut(s) 81, 1022
SchI GAGTC 3 cut(s) 265, 862, 974
ScrFI CCNGG 1 cut(s) 920
SduI GDGCHC 2 cut(s) 781, 864
SfaNI GCATC 3 cut(s) 121, 619, 1017
SinI GGWCC 1 cut(s) 420
SmiMI CAYNNNNRTG 1 cut(s) 696
SmlI CTYRAG 1 cut(s) 554
SmoI CTYRAG 1 cut(s) 554
Sse9I AATT 5 cut(s) 206, 278, 577, 749, 1044
SseBI AGGCCT 1 cut(s) 664
SsiI CCGC 3 cut(s) 15, 329, 568
SspI AATATT 2 cut(s) 347, 367
SspMI CTAG 3 cut(s) 509, 633, 660
StuI AGGCCT 1 cut(s) 664
StyD4I CCNGG 1 cut(s) 918
StyI CCWWGG 3 cut(s) 262, 900, 974
TaaI ACNGT 5 cut(s) 52, 312, 442, 550, 846
TaiI ACGT 1 cut(s) 1005
TaqI TCGA 3 cut(s) 30, 360, 1017
TaqII GACCGA 2 cut(s) 437, 780
TasI AATT 5 cut(s) 206, 278, 577, 749, 1044
TatI WGTACW 3 cut(s) 79, 981, 1020
TfiI GAWTC 1 cut(s) 923
Tru1I TTAA 5 cut(s) 230, 258, 555, 707, 807
Tru9I TTAA 5 cut(s) 230, 258, 555, 707, 807
TseI GCWGC 2 cut(s) 403, 1052
TspDTI ATGAA 2 cut(s) 136, 770
TspGWI ACGGA 1 cut(s) 284
Tth111I GACNNNGTC 1 cut(s) 290
Vha464I CTTAAG 1 cut(s) 554
VneI GTGCAC 1 cut(s) 860
VpaK11BI GGWCC 1 cut(s) 420
VspI ATTAAT 2 cut(s) 707, 807
XapI RAATTY 3 cut(s) 206, 278, 577
XbaI TCTAGA 1 cut(s) 508
XceI RCATGY 1 cut(s) 695
XmiI GTMKAC 1 cut(s) 360
XmnI GAANNNNTTC 1 cut(s) 744
XspI CTAG 3 cut(s) 509, 633, 660
ZrmI AGTACT 2 cut(s) 81, 1022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.