Belongs to the iron ascorbate-dependent oxidoreductase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 283 |
| AccI | GTMKAC | 1 cut(s) | 555 |
| AccII | CGCG | 2 cut(s) | 763, 867 |
| AciI | CCGC | 1 cut(s) | 763 |
| AclWI | GGATC | 1 cut(s) | 904 |
| AcsI | RAATTY | 3 cut(s) | 401, 473, 772 |
| AcuI | CTGAAG | 1 cut(s) | 288 |
| AfaI | GTAC | 3 cut(s) | 279, 870, 1178 |
| AflII | CTTAAG | 1 cut(s) | 749 |
| AluBI | AGCT | 4 cut(s) | 140, 422, 661, 974 |
| AluI | AGCT | 4 cut(s) | 140, 422, 661, 974 |
| Alw21I | GWGCWC | 2 cut(s) | 125, 1059 |
| Alw26I | GTCTC | 2 cut(s) | 374, 1118 |
| Alw44I | GTGCAC | 1 cut(s) | 1055 |
| AlwI | GGATC | 1 cut(s) | 904 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 743 |
| AoxI | GGCC | 4 cut(s) | 548, 671, 857, 1166 |
| ApaLI | GTGCAC | 1 cut(s) | 1055 |
| ApeKI | GCWGC | 4 cut(s) | 137, 598, 1100, 1247 |
| ApoI | RAATTY | 3 cut(s) | 401, 473, 772 |
| AseI | ATTAAT | 1 cut(s) | 1002 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 939 |
| AspLEI | GCGC | 2 cut(s) | 657, 763 |
| AspS9I | GGNCC | 1 cut(s) | 858 |
| AsuC2I | CCSGG | 1 cut(s) | 1115 |
| AsuHPI | GGTGA | 4 cut(s) | 8, 16, 898, 1022 |
| AxyI | CCTNAGG | 1 cut(s) | 987 |
| BaeGI | GKGCMC | 1 cut(s) | 1059 |
| BanI | GGYRCC | 1 cut(s) | 283 |
| BanII | GRGCYC | 1 cut(s) | 1052 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 261, 293 |
| BbsI | GAAGAC | 2 cut(s) | 932, 1240 |
| Bbv12I | GWGCWC | 2 cut(s) | 125, 1059 |
| BbvI | GCAGC | 3 cut(s) | 149, 610, 1087 |
| BccI | CCATC | 3 cut(s) | 302, 337, 887 |
| BciVI | GTATCC | 1 cut(s) | 691 |
| BclI | TGATCA | 2 cut(s) | 958, 1012 |
| BcnI | CCSGG | 1 cut(s) | 1115 |
| BcoDI | GTCTC | 2 cut(s) | 374, 1118 |
| BfaI | CTAG | 3 cut(s) | 704, 828, 872 |
| BfrI | CTTAAG | 1 cut(s) | 749 |
| BfuI | GTATCC | 1 cut(s) | 691 |
| BisI | GCNGC | 4 cut(s) | 138, 599, 1101, 1248 |
| BlsI | GCNGC | 4 cut(s) | 139, 600, 1102, 1249 |
| BmcAI | AGTACT | 1 cut(s) | 279 |
| Bme1390I | CCNGG | 1 cut(s) | 1115 |
| BmgT120I | GGNCC | 1 cut(s) | 858 |
| BmiI | GGNNCC | 1 cut(s) | 285 |
| BmrFI | CCNGG | 1 cut(s) | 1115 |
| BmrI | ACTGGG | 1 cut(s) | 1046 |
| BmsI | GCATC | 2 cut(s) | 814, 1212 |
| BmuI | ACTGGG | 1 cut(s) | 1046 |
| BpiI | GAAGAC | 2 cut(s) | 932, 1240 |
| BpuEI | CTTGAG | 1 cut(s) | 647 |
| BpuMI | CCSGG | 1 cut(s) | 1115 |
| BsaI | GGTCTC | 1 cut(s) | 374 |
| BsaJI | CCNNGG | 3 cut(s) | 222, 457, 1169 |
| Bse1I | ACTGG | 5 cut(s) | 285, 353, 551, 717, 1052 |
| Bse21I | CCTNAGG | 1 cut(s) | 987 |
| BseDI | CCNNGG | 3 cut(s) | 222, 457, 1169 |
| BseGI | GGATG | 3 cut(s) | 166, 352, 829 |
| BseMII | CTCAG | 3 cut(s) | 140, 427, 978 |
| BseNI | ACTGG | 5 cut(s) | 285, 353, 551, 717, 1052 |
| BseRI | GAGGAG | 1 cut(s) | 362 |
| BseSI | GKGCMC | 1 cut(s) | 1059 |
| BseXI | GCAGC | 3 cut(s) | 149, 610, 1087 |
| Bsh1236I | CGCG | 2 cut(s) | 763, 867 |
| Bsh1285I | CGRYCG | 1 cut(s) | 865 |
| BshFI | GGCC | 4 cut(s) | 550, 673, 859, 1168 |
| BshNI | GGYRCC | 1 cut(s) | 283 |
| BsiEI | CGRYCG | 1 cut(s) | 865 |
| BsiHKAI | GWGCWC | 2 cut(s) | 125, 1059 |
| BsiSI | CCGG | 1 cut(s) | 1115 |
| BslFI | GGGAC | 1 cut(s) | 1097 |
| BsmAI | GTCTC | 2 cut(s) | 374, 1118 |
| BsmFI | GGGAC | 1 cut(s) | 1097 |
| BsnI | GGCC | 4 cut(s) | 550, 673, 859, 1168 |
| Bso31I | GGTCTC | 1 cut(s) | 374 |
| Bsp1286I | GDGCHC | 3 cut(s) | 125, 1052, 1059 |
| Bsp1407I | TGTACA | 1 cut(s) | 1176 |
| Bsp143I | GATC | 8 cut(s) | 3, 64, 361, 416, 862, 896, 958, 1012 |
| Bsp19I | CCATGG | 1 cut(s) | 457 |
| BspACI | CCGC | 1 cut(s) | 763 |
| BspANI | GGCC | 4 cut(s) | 550, 673, 859, 1168 |
| BspCNI | CTCAG | 3 cut(s) | 139, 426, 979 |
| BspFNI | CGCG | 2 cut(s) | 763, 867 |
| BspLI | GGNNCC | 1 cut(s) | 285 |
| BspPI | GGATC | 1 cut(s) | 904 |
| BspT107I | GGYRCC | 1 cut(s) | 283 |
| BspTI | CTTAAG | 1 cut(s) | 749 |
| BspTNI | GGTCTC | 1 cut(s) | 374 |
| BsrGI | TGTACA | 1 cut(s) | 1176 |
| BsrI | ACTGG | 5 cut(s) | 285, 353, 551, 717, 1052 |
| BssECI | CCNNGG | 3 cut(s) | 222, 457, 1169 |
| BssMI | GATC | 8 cut(s) | 3, 64, 361, 416, 862, 896, 958, 1012 |
| BssT1I | CCWWGG | 3 cut(s) | 222, 457, 1169 |
| Bst4CI | ACNGT | 4 cut(s) | 250, 637, 745, 1041 |
| BstAFI | CTTAAG | 1 cut(s) | 749 |
| BstAUI | TGTACA | 1 cut(s) | 1176 |
| BstC8I | GCNNGC | 1 cut(s) | 659 |
| BstDEI | CTNAG | 3 cut(s) | 126, 413, 987 |
| BstDSI | CCRYGG | 1 cut(s) | 457 |
| BstF5I | GGATG | 3 cut(s) | 166, 352, 829 |
| BstFNI | CGCG | 2 cut(s) | 763, 867 |
| BstHHI | GCGC | 2 cut(s) | 657, 763 |
| BstKTI | GATC | 8 cut(s) | 6, 67, 364, 419, 865, 899, 961, 1015 |
| BstMAI | GTCTC | 2 cut(s) | 374, 1118 |
| BstMBI | GATC | 8 cut(s) | 3, 64, 361, 416, 862, 896, 958, 1012 |
| BstMCI | CGRYCG | 1 cut(s) | 865 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 146, 532 |
| BstNSI | RCATGY | 1 cut(s) | 890 |
| BstSCI | CCNGG | 1 cut(s) | 1113 |
| BstSLI | GKGCMC | 1 cut(s) | 1059 |
| BstUI | CGCG | 2 cut(s) | 763, 867 |
| BstV1I | GCAGC | 3 cut(s) | 149, 610, 1087 |
| BstV2I | GAAGAC | 2 cut(s) | 932, 1240 |
| Bsu36I | CCTNAGG | 1 cut(s) | 987 |
| BsuI | GTATCC | 1 cut(s) | 691 |
| BsuRI | GGCC | 4 cut(s) | 550, 673, 859, 1168 |
| BtgI | CCRYGG | 1 cut(s) | 457 |
| BtsCI | GGATG | 3 cut(s) | 166, 352, 829 |
| BtsI | GCAGTG | 1 cut(s) | 521 |
| BtsIMutI | CAGTG | 3 cut(s) | 264, 521, 1059 |
| Cac8I | GCNNGC | 1 cut(s) | 659 |
| CaiI | CAGNNNCTG | 1 cut(s) | 743 |
| CfoI | GCGC | 2 cut(s) | 657, 763 |
| Cfr13I | GGNCC | 1 cut(s) | 858 |
| Csp6I | GTAC | 3 cut(s) | 278, 869, 1177 |
| CviAII | CATG | 5 cut(s) | 199, 458, 583, 757, 887 |
| CviQI | GTAC | 3 cut(s) | 278, 869, 1177 |
| DdeI | CTNAG | 3 cut(s) | 126, 413, 987 |
| DpnI | GATC | 8 cut(s) | 5, 66, 363, 418, 864, 898, 960, 1014 |
| DpnII | GATC | 8 cut(s) | 3, 64, 361, 416, 862, 896, 958, 1012 |
| Eco130I | CCWWGG | 3 cut(s) | 222, 457, 1169 |
| Eco24I | GRGCYC | 1 cut(s) | 1052 |
| Eco31I | GGTCTC | 1 cut(s) | 374 |
| Eco57I | CTGAAG | 1 cut(s) | 288 |
| Eco81I | CCTNAGG | 1 cut(s) | 987 |
| EcoRI | GAATTC | 1 cut(s) | 473 |
| EcoT14I | CCWWGG | 3 cut(s) | 222, 457, 1169 |
| EcoT38I | GRGCYC | 1 cut(s) | 1052 |
| ErhI | CCWWGG | 3 cut(s) | 222, 457, 1169 |
| FaeI | CATG | 5 cut(s) | 202, 461, 586, 760, 890 |
| FaqI | GGGAC | 1 cut(s) | 1097 |
| FatI | CATG | 5 cut(s) | 198, 457, 582, 756, 886 |
| FbaI | TGATCA | 2 cut(s) | 958, 1012 |
| FblI | GTMKAC | 1 cut(s) | 555 |
| Fnu4HI | GCNGC | 4 cut(s) | 138, 599, 1101, 1248 |
| FokI | GGATG | 3 cut(s) | 153, 359, 836 |
| FriOI | GRGCYC | 1 cut(s) | 1052 |
| Fsp4HI | GCNGC | 4 cut(s) | 138, 599, 1101, 1248 |
| FspBI | CTAG | 3 cut(s) | 704, 828, 872 |
| GlaI | GCGC | 2 cut(s) | 656, 762 |
| GluI | GCNGC | 4 cut(s) | 138, 599, 1101, 1248 |
| HaeIII | GGCC | 4 cut(s) | 550, 673, 859, 1168 |
| HapII | CCGG | 1 cut(s) | 1115 |
| HhaI | GCGC | 2 cut(s) | 657, 763 |
| Hin1II | CATG | 5 cut(s) | 202, 461, 586, 760, 890 |
| Hin6I | GCGC | 2 cut(s) | 655, 761 |
| HinP1I | GCGC | 2 cut(s) | 655, 761 |
| HincII | GTYRAC | 1 cut(s) | 556 |
| HindII | GTYRAC | 1 cut(s) | 556 |
| HinfI | GANTC | 5 cut(s) | 206, 312, 466, 1118, 1160 |
| HpaII | CCGG | 1 cut(s) | 1115 |
| HphI | GGTGA | 4 cut(s) | 8, 16, 898, 1022 |
| Hpy166II | GTNNAC | 2 cut(s) | 556, 1057 |
| Hpy188I | TCNGA | 6 cut(s) | 64, 69, 416, 595, 951, 1123 |
| Hpy188III | TCNNGA | 6 cut(s) | 470, 499, 704, 794, 986, 1045 |
| Hpy8I | GTNNAC | 2 cut(s) | 556, 1057 |
| HpyAV | CCTTC | 5 cut(s) | 263, 390, 560, 920, 929 |
| HpyCH4III | ACNGT | 4 cut(s) | 250, 637, 745, 1041 |
| HpyCH4IV | ACGT | 1 cut(s) | 1197 |
| HpyCH4V | TGCA | 8 cut(s) | 149, 253, 515, 526, 890, 919, 1057, 1076 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 146, 532 |
| HpyF3I | CTNAG | 3 cut(s) | 126, 413, 987 |
| HpySE526I | ACGT | 1 cut(s) | 1197 |
| Hsp92II | CATG | 5 cut(s) | 202, 461, 586, 760, 890 |
| HspAI | GCGC | 2 cut(s) | 655, 761 |
| Ksp22I | TGATCA | 2 cut(s) | 958, 1012 |
| Kzo9I | GATC | 8 cut(s) | 3, 64, 361, 416, 862, 896, 958, 1012 |
| Lsp1109I | GCAGC | 3 cut(s) | 149, 610, 1087 |
| LweI | GCATC | 2 cut(s) | 814, 1212 |
| MaeI | CTAG | 3 cut(s) | 704, 828, 872 |
| MaeII | ACGT | 1 cut(s) | 1197 |
| MaeIII | GTNAC | 2 cut(s) | 723, 1066 |
| MalI | GATC | 8 cut(s) | 5, 66, 363, 418, 864, 898, 960, 1014 |
| MboI | GATC | 8 cut(s) | 3, 64, 361, 416, 862, 896, 958, 1012 |
| MboII | GAAGA | 7 cut(s) | 81, 201, 410, 794, 932, 1242, 1245 |
| MfeI | CAATTG | 2 cut(s) | 978, 1239 |
| MhlI | GDGCHC | 3 cut(s) | 125, 1052, 1059 |
| MlyI | GAGTC | 2 cut(s) | 460, 1169 |
| MroXI | GAANNNNTTC | 1 cut(s) | 939 |
| MseI | TTAA | 3 cut(s) | 750, 834, 1002 |
| MslI | CAYNNNNRTG | 1 cut(s) | 891 |
| MspCI | CTTAAG | 1 cut(s) | 749 |
| MspI | CCGG | 1 cut(s) | 1115 |
| MspR9I | CCNGG | 1 cut(s) | 1115 |
| MunI | CAATTG | 2 cut(s) | 978, 1239 |
| MvnI | CGCG | 2 cut(s) | 763, 867 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 146, 532 |
| NciI | CCSGG | 1 cut(s) | 1115 |
| NcoI | CCATGG | 1 cut(s) | 457 |
| NdeII | GATC | 8 cut(s) | 3, 64, 361, 416, 862, 896, 958, 1012 |
| NlaIII | CATG | 5 cut(s) | 202, 461, 586, 760, 890 |
| NlaIV | GGNNCC | 1 cut(s) | 285 |
| NmuCI | GTSAC | 1 cut(s) | 1066 |
| NspI | RCATGY | 1 cut(s) | 890 |
| PdmI | GAANNNNTTC | 1 cut(s) | 939 |
| PfeI | GAWTC | 3 cut(s) | 206, 312, 1118 |
| PflFI | GACNNNGTC | 1 cut(s) | 485 |
| PfoI | TCCNGGA | 1 cut(s) | 1113 |
| PkrI | GCNGC | 4 cut(s) | 139, 600, 1102, 1249 |
| Ple19I | CGATCG | 1 cut(s) | 865 |
| PleI | GAGTC | 2 cut(s) | 460, 1168 |
| PpsI | GAGTC | 2 cut(s) | 460, 1168 |
| PshBI | ATTAAT | 1 cut(s) | 1002 |
| PspN4I | GGNNCC | 1 cut(s) | 285 |
| PspPI | GGNCC | 1 cut(s) | 858 |
| PstNI | CAGNNNCTG | 1 cut(s) | 743 |
| PsyI | GACNNNGTC | 1 cut(s) | 485 |
| PvuI | CGATCG | 1 cut(s) | 865 |
| RsaI | GTAC | 3 cut(s) | 279, 870, 1178 |
| RsaNI | GTAC | 3 cut(s) | 278, 869, 1177 |
| RseI | CAYNNNNRTG | 1 cut(s) | 891 |
| SalI | GTCGAC | 1 cut(s) | 554 |
| SaqAI | TTAA | 3 cut(s) | 750, 834, 1002 |
| SatI | GCNGC | 4 cut(s) | 138, 599, 1101, 1248 |
| Sau3AI | GATC | 8 cut(s) | 3, 64, 361, 416, 862, 896, 958, 1012 |
| Sau96I | GGNCC | 1 cut(s) | 858 |
| ScaI | AGTACT | 1 cut(s) | 279 |
| SchI | GAGTC | 2 cut(s) | 460, 1169 |
| ScrFI | CCNGG | 1 cut(s) | 1115 |
| SduI | GDGCHC | 3 cut(s) | 125, 1052, 1059 |
| SfaNI | GCATC | 2 cut(s) | 814, 1212 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 891 |
| SmlI | CTYRAG | 2 cut(s) | 662, 749 |
| SmoI | CTYRAG | 2 cut(s) | 662, 749 |
| SsiI | CCGC | 1 cut(s) | 763 |
| SspI | AATATT | 1 cut(s) | 542 |
| SspMI | CTAG | 3 cut(s) | 704, 828, 872 |
| StyD4I | CCNGG | 1 cut(s) | 1113 |
| StyI | CCWWGG | 3 cut(s) | 222, 457, 1169 |
| TaaI | ACNGT | 4 cut(s) | 250, 637, 745, 1041 |
| TaiI | ACGT | 1 cut(s) | 1200 |
| TaqI | TCGA | 2 cut(s) | 228, 555 |
| TatI | WGTACW | 2 cut(s) | 277, 1176 |
| TfiI | GAWTC | 3 cut(s) | 206, 312, 1118 |
| Tru1I | TTAA | 3 cut(s) | 750, 834, 1002 |
| Tru9I | TTAA | 3 cut(s) | 750, 834, 1002 |
| TscAI | CASTG | 3 cut(s) | 271, 528, 1059 |
| TseFI | GTSAC | 1 cut(s) | 1066 |
| TseI | GCWGC | 4 cut(s) | 137, 598, 1100, 1247 |
| Tsp45I | GTSAC | 1 cut(s) | 1066 |
| TspGWI | ACGGA | 1 cut(s) | 479 |
| TspRI | CASTG | 3 cut(s) | 271, 528, 1059 |
| Tth111I | GACNNNGTC | 1 cut(s) | 485 |
| Vha464I | CTTAAG | 1 cut(s) | 749 |
| VneI | GTGCAC | 1 cut(s) | 1055 |
| VspI | ATTAAT | 1 cut(s) | 1002 |
| XapI | RAATTY | 3 cut(s) | 401, 473, 772 |
| XbaI | TCTAGA | 1 cut(s) | 703 |
| XceI | RCATGY | 1 cut(s) | 890 |
| XmiI | GTMKAC | 1 cut(s) | 555 |
| XmnI | GAANNNNTTC | 1 cut(s) | 939 |
| XspI | CTAG | 3 cut(s) | 704, 828, 872 |
| ZrmI | AGTACT | 1 cut(s) | 279 |