FvH4_1g18830

tRNA-dihydrouridine20 synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001304
Physical Location & Seq
Forward (+)
11110482 .. 11116277
5796 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g18830.t1

Sequence Viewer

Length: 669 bp
ATGTCGGATTCTGAATTGGAGGACAAGGTTTTCAGCTTGTTCAAGGATTTTATGGCCGGGATTGCCAAGTTTGAGGAATTGGTGCCCATGGGAAGCACATTTCTCAATGGTTTTCAGCATGCACTGGAGTTTCTTCGACGGCCGCCGATCGATAAAACATCTCAGTTGGTTAAAAACATAATCCAATCTAACGAAACAAAGAGGGTTAAATCATACATTGAAGCTGGATGTATCAACACTGATGATGGCAGGCACAATATAACCAAGTTGCAATCATGCCAACTTGGACTTCATGACCATATACGTAAAGGAAAATGTATACTTAACGAACTGGAATGCCTACTGGAAGATGTAAGGGGTGCAATGCAAGATATATCCTTGTTCCAGGATGACAATTTGGGTGAAAGGTTGAATGAACAAGCCTGTATCAATGACAAGGAGGAATTTGCCTTGCCTTGTCCCAGAAGAAATGAAGTTTCTGATTATGCTGTCCTGATGGCAGTCATTTATAGTATGGTCACGCAAGAATATATGATGCAGGAAAGAGTTGTGTCTGCTTTAAGTCTCAAATTATCTTCAGGAGAATTAGACAGCTACTGTCTTATGTGGTCTTTGCGTCCTTACATAGACGAAGAGATTATGGGTCATGCTTGGAAACTTATATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

223

Amino Acids

25.44

Weight (kDa)

4.96

Isoelectric Point (pI)

58.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7795 PF25071 4 - 124 3.5e-56 Domain of unknown function (DUF7795)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 82
AccI GTMKAC 1 cut(s) 319
AciI CCGC 1 cut(s) 143
AcoI YGGCCR 2 cut(s) 54, 140
AcsI RAATTY 1 cut(s) 443
AcuI CTGAAG 1 cut(s) 561
AgsI TTSAA 3 cut(s) 43, 221, 412
AjnI CCWGG 1 cut(s) 384
AjuI GAANNNNNNNTTGG 2 cut(s) 273, 305
AluBI AGCT 3 cut(s) 36, 224, 594
AluI AGCT 3 cut(s) 36, 224, 594
Alw26I GTCTC 1 cut(s) 569
AlwNI CAGNNNCTG 1 cut(s) 597
AoxI GGCC 2 cut(s) 54, 140
ApoI RAATTY 1 cut(s) 443
AsuC2I CCSGG 1 cut(s) 58
AsuHPI GGTGA 1 cut(s) 413
BaeGI GKGCMC 1 cut(s) 87
BanI GGYRCC 1 cut(s) 82
BccI CCATC 2 cut(s) 239, 490
BceAI ACGGC 1 cut(s) 155
BciT130I CCWGG 1 cut(s) 386
BcnI CCSGG 1 cut(s) 58
BcoDI GTCTC 1 cut(s) 569
BisI GCNGC 1 cut(s) 143
BlsI GCNGC 1 cut(s) 144
Bme1390I CCNGG 2 cut(s) 58, 386
BmiI GGNNCC 1 cut(s) 84
BmrFI CCNGG 2 cut(s) 58, 386
BmsI GCATC 1 cut(s) 525
BpmI CTGGAG 1 cut(s) 146
BpuMI CCSGG 1 cut(s) 58
Bsa29I ATCGAT 1 cut(s) 150
BsaAI YACGTR 1 cut(s) 305
BsaJI CCNNGG 1 cut(s) 87
Bse1I ACTGG 3 cut(s) 129, 336, 348
Bse3DI GCAATG 1 cut(s) 369
BseBI CCWGG 1 cut(s) 386
BseCI ATCGAT 1 cut(s) 150
BseDI CCNNGG 1 cut(s) 87
BseGI GGATG 2 cut(s) 233, 394
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 1 cut(s) 176
BseNI ACTGG 3 cut(s) 129, 336, 348
BseSI GKGCMC 1 cut(s) 87
BseX3I CGGCCG 1 cut(s) 140
Bsh1285I CGRYCG 2 cut(s) 143, 150
BshFI GGCC 2 cut(s) 56, 142
BshNI GGYRCC 1 cut(s) 82
BshVI ATCGAT 1 cut(s) 150
BsiEI CGRYCG 2 cut(s) 143, 150
BsiSI CCGG 1 cut(s) 57
BslFI GGGAC 1 cut(s) 444
BsmAI GTCTC 1 cut(s) 569
BsmFI GGGAC 1 cut(s) 444
BsmI GAATGC 1 cut(s) 341
BsnI GGCC 2 cut(s) 56, 142
Bsp1286I GDGCHC 1 cut(s) 87
Bsp143I GATC 1 cut(s) 147
Bsp19I CCATGG 1 cut(s) 87
BspACI CCGC 1 cut(s) 143
BspANI GGCC 2 cut(s) 56, 142
BspCNI CTCAG 1 cut(s) 175
BspDI ATCGAT 1 cut(s) 150
BspHI TCATGA 1 cut(s) 292
BspLI GGNNCC 1 cut(s) 84
BspT107I GGYRCC 1 cut(s) 82
BsrDI GCAATG 1 cut(s) 369
BsrI ACTGG 3 cut(s) 129, 336, 348
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 1 cut(s) 147
BssNAI GTATAC 1 cut(s) 320
BssT1I CCWWGG 1 cut(s) 87
Bst1107I GTATAC 1 cut(s) 320
Bst2UI CCWGG 1 cut(s) 386
Bst4CI ACNGT 1 cut(s) 599
Bst6I CTCTTC 1 cut(s) 627
BstBAI YACGTR 1 cut(s) 305
BstC8I GCNNGC 2 cut(s) 120, 251
BstDEI CTNAG 1 cut(s) 162
BstDSI CCRYGG 1 cut(s) 87
BstF5I GGATG 2 cut(s) 233, 394
BstKTI GATC 1 cut(s) 150
BstMAI GTCTC 1 cut(s) 569
BstMBI GATC 1 cut(s) 147
BstMCI CGRYCG 2 cut(s) 143, 150
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstNI CCWGG 1 cut(s) 386
BstNSI RCATGY 1 cut(s) 122
BstSCI CCNGG 2 cut(s) 56, 384
BstSLI GKGCMC 1 cut(s) 87
BstSNI TACGTA 1 cut(s) 305
BstZ17I GTATAC 1 cut(s) 320
BstZI CGGCCG 1 cut(s) 140
Bsu15I ATCGAT 1 cut(s) 150
BsuRI GGCC 2 cut(s) 56, 142
BsuTUI ATCGAT 1 cut(s) 150
BtgI CCRYGG 1 cut(s) 87
BtsCI GGATG 2 cut(s) 233, 394
BtsIMutI CAGTG 2 cut(s) 122, 237
Cac8I GCNNGC 2 cut(s) 120, 251
CaiI CAGNNNCTG 1 cut(s) 597
CciI TCATGA 1 cut(s) 292
ClaI ATCGAT 1 cut(s) 150
CseI GACGC 1 cut(s) 605
CviAII CATG 5 cut(s) 88, 119, 276, 293, 647
CviJI RGCY 6 cut(s) 36, 56, 142, 224, 422, 594
CviKI_1 RGCY 6 cut(s) 36, 56, 142, 224, 422, 594
DdeI CTNAG 1 cut(s) 162
DpnI GATC 1 cut(s) 149
DpnII GATC 1 cut(s) 147
EaeI YGGCCR 2 cut(s) 54, 140
EagI CGGCCG 1 cut(s) 140
Eam1104I CTCTTC 1 cut(s) 627
EarI CTCTTC 1 cut(s) 627
EclXI CGGCCG 1 cut(s) 140
Eco105I TACGTA 1 cut(s) 305
Eco130I CCWWGG 1 cut(s) 87
Eco52I CGGCCG 1 cut(s) 140
Eco57I CTGAAG 1 cut(s) 561
EcoRII CCWGG 1 cut(s) 384
EcoT14I CCWWGG 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 5 cut(s) 91, 122, 279, 296, 650
FaqI GGGAC 1 cut(s) 444
FatI CATG 5 cut(s) 87, 118, 275, 292, 646
FblI GTMKAC 1 cut(s) 319
Fnu4HI GCNGC 1 cut(s) 143
FokI GGATG 2 cut(s) 240, 401
Fsp4HI GCNGC 1 cut(s) 143
GluI GCNGC 1 cut(s) 143
GsuI CTGGAG 1 cut(s) 146
HaeIII GGCC 2 cut(s) 56, 142
HapII CCGG 1 cut(s) 57
HgaI GACGC 1 cut(s) 605
Hin1II CATG 5 cut(s) 91, 122, 279, 296, 650
HinfI GANTC 1 cut(s) 8
HpaII CCGG 1 cut(s) 57
HphI GGTGA 1 cut(s) 413
Hpy166II GTNNAC 1 cut(s) 320
Hpy188I TCNGA 3 cut(s) 7, 13, 481
Hpy188III TCNNGA 3 cut(s) 293, 493, 579
Hpy8I GTNNAC 1 cut(s) 320
Hpy99I CGWCG 1 cut(s) 141
HpyCH4III ACNGT 1 cut(s) 599
HpyCH4IV ACGT 1 cut(s) 304
HpyCH4V TGCA 5 cut(s) 122, 271, 362, 367, 538
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpyF3I CTNAG 1 cut(s) 162
HpySE526I ACGT 1 cut(s) 304
Hsp92II CATG 5 cut(s) 91, 122, 279, 296, 650
Kzo9I GATC 1 cut(s) 147
LweI GCATC 1 cut(s) 525
MaeII ACGT 1 cut(s) 304
MaeIII GTNAC 1 cut(s) 517
MalI GATC 1 cut(s) 149
MboI GATC 1 cut(s) 147
MboII GAAGA 5 cut(s) 125, 359, 477, 567, 644
MhlI GDGCHC 1 cut(s) 87
MluCI AATT 6 cut(s) 14, 77, 394, 443, 569, 584
MnlI CCTC 4 cut(s) 13, 67, 195, 433
MseI TTAA 4 cut(s) 171, 207, 324, 560
MspI CCGG 1 cut(s) 57
MspR9I CCNGG 2 cut(s) 58, 386
Mva1269I GAATGC 1 cut(s) 341
MvaI CCWGG 1 cut(s) 386
MwoI GCNNNNNNNGC 1 cut(s) 62
NciI CCSGG 1 cut(s) 58
NcoI CCATGG 1 cut(s) 87
NdeII GATC 1 cut(s) 147
NlaIII CATG 5 cut(s) 91, 122, 279, 296, 650
NlaIV GGNNCC 1 cut(s) 84
NmuCI GTSAC 1 cut(s) 517
NspI RCATGY 1 cut(s) 122
PaeI GCATGC 1 cut(s) 122
PagI TCATGA 1 cut(s) 292
PctI GAATGC 1 cut(s) 341
PfeI GAWTC 1 cut(s) 8
PfoI TCCNGGA 1 cut(s) 384
PkrI GCNGC 1 cut(s) 144
Ple19I CGATCG 1 cut(s) 150
Ppu21I YACGTR 1 cut(s) 305
Psp6I CCWGG 1 cut(s) 384
PspGI CCWGG 1 cut(s) 384
PspN4I GGNNCC 1 cut(s) 84
PstNI CAGNNNCTG 1 cut(s) 597
PvuI CGATCG 1 cut(s) 150
SaqAI TTAA 4 cut(s) 171, 207, 324, 560
SatI GCNGC 1 cut(s) 143
Sau3AI GATC 1 cut(s) 147
ScrFI CCNGG 2 cut(s) 58, 386
SduI GDGCHC 1 cut(s) 87
SetI ASST 6 cut(s) 30, 38, 226, 307, 410, 596
SfaNI GCATC 1 cut(s) 525
SnaBI TACGTA 1 cut(s) 305
SphI GCATGC 1 cut(s) 122
Sse9I AATT 6 cut(s) 14, 77, 394, 443, 569, 584
SsiI CCGC 1 cut(s) 143
StyD4I CCNGG 2 cut(s) 56, 384
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 1 cut(s) 599
TaiI ACGT 1 cut(s) 307
TaqI TCGA 2 cut(s) 136, 150
TasI AATT 6 cut(s) 14, 77, 394, 443, 569, 584
TauI GCSGC 1 cut(s) 145
TfiI GAWTC 1 cut(s) 8
Tru1I TTAA 4 cut(s) 171, 207, 324, 560
Tru9I TTAA 4 cut(s) 171, 207, 324, 560
TscAI CASTG 2 cut(s) 129, 244
TseFI GTSAC 1 cut(s) 517
Tsp45I GTSAC 1 cut(s) 517
TspDTI ATGAA 3 cut(s) 281, 429, 486
TspRI CASTG 2 cut(s) 129, 244
XapI RAATTY 1 cut(s) 443
XceI RCATGY 1 cut(s) 122
XmiI GTMKAC 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.