Rmu_sc0000507.1_g000008

tRNA-dihydrouridine20 synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000507.1
OG0001304
Physical Location & Seq
Forward (+)
50872 .. 53347
2476 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000507.1_g000008.1.cds

Sequence Viewer

Length: 732 bp
atgagagtgtattttgctttgaggtttatcacctgcggatttgcagttcttgctgttaggatgtcgaattctgaattagaggacaagattttcaacttgttcaaggattttatggtcgggattgccaagtttgaggaattggtgcccatgggaagcccatttctcaatggctttcagcatgcactggagtttcttcgacggccggcgatagataaaacatctgagttagttaaaaatataatccaatcaaacgaaacaaggagggttaaatcatacattgaagctggatgtatcaacactgatgatggcaggcacaatataaccaagttagtgcaatcatgccaacttggacttcatgaccatatatgtaaaggaaaatgtatacttaacgaactggaatgcctactagaagatgtaagtggtgcaatgcaagataaatcattgttccaggatgacaatttgggtgaaaggttgaatgaacaagcatgtatcaatgacaaggaggaaattgcctcgccttgtcccagaagaaatgaagttactgattatgctgtcctgatggcagtcatttatcgtatggtcaagcaagaatatatgatgcaggaaagaattgtttctgcgttaagtctcaagtcatcttccggagaattagagagctactcccttatgtggtctttgcgtccttacatagatgaagaaattatgggtcttgcttggaaacttatacactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

243

Amino Acids

27.86

Weight (kDa)

5.36

Isoelectric Point (pI)

57.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 41
Acc36I ACCTGC 1 cut(s) 41
AccB1I GGYRCC 1 cut(s) 142
AccI GTMKAC 1 cut(s) 382
AccIII TCCGGA 1 cut(s) 641
AciI CCGC 1 cut(s) 36
AcoI YGGCCR 1 cut(s) 200
AcsI RAATTY 1 cut(s) 67
AfiI CCNNNNNNNGG 1 cut(s) 669
AgsI TTSAA 4 cut(s) 94, 103, 281, 475
AjnI CCWGG 1 cut(s) 447
AjuI GAANNNNNNNTTGG 2 cut(s) 336, 368
AloI GAACNNNNNNTCC 2 cut(s) 30, 62
AluBI AGCT 2 cut(s) 284, 657
AluI AGCT 2 cut(s) 284, 657
Alw26I GTCTC 1 cut(s) 632
Aor13HI TCCGGA 1 cut(s) 641
AoxI GGCC 1 cut(s) 200
ApoI RAATTY 1 cut(s) 67
Asp700I GAANNNNTTC 1 cut(s) 613
AsuHPI GGTGA 2 cut(s) 22, 476
BaeGI GKGCMC 1 cut(s) 147
BanI GGYRCC 1 cut(s) 142
BccI CCATC 2 cut(s) 299, 553
BceAI ACGGC 1 cut(s) 215
BciT130I CCWGG 1 cut(s) 449
BcoDI GTCTC 1 cut(s) 632
BfaI CTAG 1 cut(s) 407
BfuAI ACCTGC 1 cut(s) 41
Bme1390I CCNGG 1 cut(s) 449
BmiI GGNNCC 1 cut(s) 144
BmrFI CCNGG 1 cut(s) 449
BmsI GCATC 1 cut(s) 588
BplI GAGNNNNNCTC 2 cut(s) 644, 676
BpmI CTGGAG 1 cut(s) 206
BpuEI CTTGAG 1 cut(s) 614
BsaJI CCNNGG 1 cut(s) 147
BsaWI WCCGGW 1 cut(s) 641
Bsc4I CCNNNNNNNGG 1 cut(s) 669
Bse118I RCCGGY 1 cut(s) 202
Bse1I ACTGG 2 cut(s) 189, 399
Bse3DI GCAATG 1 cut(s) 432
BseAI TCCGGA 1 cut(s) 641
BseBI CCWGG 1 cut(s) 449
BseDI CCNNGG 1 cut(s) 147
BseGI GGATG 3 cut(s) 66, 293, 457
BseLI CCNNNNNNNGG 1 cut(s) 669
BseMI GCAATG 1 cut(s) 432
BseMII CTCAG 1 cut(s) 213
BseNI ACTGG 2 cut(s) 189, 399
BseSI GKGCMC 1 cut(s) 147
BseX3I CGGCCG 1 cut(s) 200
Bsh1285I CGRYCG 1 cut(s) 203
BshFI GGCC 1 cut(s) 202
BshNI GGYRCC 1 cut(s) 142
BsiEI CGRYCG 1 cut(s) 203
BsiSI CCGG 2 cut(s) 203, 642
BslFI GGGAC 1 cut(s) 507
BslI CCNNNNNNNGG 1 cut(s) 669
BsmAI GTCTC 1 cut(s) 632
BsmFI GGGAC 1 cut(s) 507
BsmI GAATGC 1 cut(s) 404
BsnI GGCC 1 cut(s) 202
Bsp1286I GDGCHC 1 cut(s) 147
Bsp13I TCCGGA 1 cut(s) 641
Bsp19I CCATGG 1 cut(s) 147
BspACI CCGC 1 cut(s) 36
BspANI GGCC 1 cut(s) 202
BspCNI CTCAG 1 cut(s) 214
BspEI TCCGGA 1 cut(s) 641
BspHI TCATGA 1 cut(s) 355
BspLI GGNNCC 1 cut(s) 144
BspMI ACCTGC 1 cut(s) 41
BspT107I GGYRCC 1 cut(s) 142
BsrDI GCAATG 1 cut(s) 432
BsrFI RCCGGY 1 cut(s) 202
BsrI ACTGG 2 cut(s) 189, 399
BssAI RCCGGY 1 cut(s) 202
BssECI CCNNGG 1 cut(s) 147
BssNAI GTATAC 1 cut(s) 383
BssT1I CCWWGG 1 cut(s) 147
Bst1107I GTATAC 1 cut(s) 383
Bst2UI CCWGG 1 cut(s) 449
BstAPI GCANNNNNTGC 1 cut(s) 50
BstC8I GCNNGC 3 cut(s) 180, 204, 311
BstDEI CTNAG 1 cut(s) 222
BstDSI CCRYGG 1 cut(s) 147
BstF5I GGATG 3 cut(s) 66, 293, 457
BstMAI GTCTC 1 cut(s) 632
BstMCI CGRYCG 1 cut(s) 203
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstNI CCWGG 1 cut(s) 449
BstNSI RCATGY 2 cut(s) 182, 489
BstSCI CCNGG 1 cut(s) 447
BstSLI GKGCMC 1 cut(s) 147
BstZ17I GTATAC 1 cut(s) 383
BstZI CGGCCG 1 cut(s) 200
BsuRI GGCC 1 cut(s) 202
BtgI CCRYGG 1 cut(s) 147
BtsCI GGATG 3 cut(s) 66, 293, 457
BtsIMutI CAGTG 3 cut(s) 182, 297, 727
BveI ACCTGC 1 cut(s) 41
Cac8I GCNNGC 3 cut(s) 180, 204, 311
CciI TCATGA 1 cut(s) 355
Cfr10I RCCGGY 1 cut(s) 202
CseI GACGC 1 cut(s) 668
CviAII CATG 5 cut(s) 148, 179, 339, 356, 486
CviJI RGCY 5 cut(s) 156, 171, 202, 284, 657
CviKI_1 RGCY 5 cut(s) 156, 171, 202, 284, 657
DdeI CTNAG 1 cut(s) 222
EaeI YGGCCR 1 cut(s) 200
EagI CGGCCG 1 cut(s) 200
EclXI CGGCCG 1 cut(s) 200
Eco130I CCWWGG 1 cut(s) 147
Eco52I CGGCCG 1 cut(s) 200
EcoRI GAATTC 1 cut(s) 67
EcoRII CCWGG 1 cut(s) 447
EcoT14I CCWWGG 1 cut(s) 147
ErhI CCWWGG 1 cut(s) 147
FaeI CATG 5 cut(s) 151, 182, 342, 359, 489
FaqI GGGAC 1 cut(s) 507
FatI CATG 5 cut(s) 147, 178, 338, 355, 485
FblI GTMKAC 1 cut(s) 382
FokI GGATG 3 cut(s) 73, 300, 464
FspBI CTAG 1 cut(s) 407
GsuI CTGGAG 1 cut(s) 206
HaeIII GGCC 1 cut(s) 202
HapII CCGG 2 cut(s) 203, 642
HgaI GACGC 1 cut(s) 668
Hin1II CATG 5 cut(s) 151, 182, 342, 359, 489
HpaII CCGG 2 cut(s) 203, 642
HphI GGTGA 2 cut(s) 22, 476
Hpy166II GTNNAC 1 cut(s) 383
Hpy188I TCNGA 2 cut(s) 73, 223
Hpy188III TCNNGA 4 cut(s) 118, 356, 556, 642
Hpy8I GTNNAC 1 cut(s) 383
Hpy99I CGWCG 1 cut(s) 201
HpyCH4V TGCA 6 cut(s) 44, 182, 334, 425, 430, 601
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
HpyF3I CTNAG 1 cut(s) 222
Hsp92II CATG 5 cut(s) 151, 182, 342, 359, 489
Kpn2I TCCGGA 1 cut(s) 641
KroI GCCGGC 1 cut(s) 202
KroNI GCCGGC 1 cut(s) 204
LweI GCATC 1 cut(s) 588
MaeI CTAG 1 cut(s) 407
MaeIII GTNAC 1 cut(s) 538
MboII GAAGA 5 cut(s) 185, 422, 540, 630, 707
MhlI GDGCHC 1 cut(s) 147
MluCI AATT 8 cut(s) 67, 74, 137, 457, 507, 609, 647, 699
MnlI CCTC 6 cut(s) 15, 73, 127, 255, 496, 523
MroI TCCGGA 1 cut(s) 641
MroNI GCCGGC 1 cut(s) 202
MroXI GAANNNNTTC 1 cut(s) 613
MseI TTAA 4 cut(s) 231, 267, 387, 623
MspI CCGG 2 cut(s) 203, 642
MspR9I CCNGG 1 cut(s) 449
Mva1269I GAATGC 1 cut(s) 404
MvaI CCWGG 1 cut(s) 449
MwoI GCNNNNNNNGC 1 cut(s) 50
NaeI GCCGGC 1 cut(s) 204
NcoI CCATGG 1 cut(s) 147
NgoMIV GCCGGC 1 cut(s) 202
NlaIII CATG 5 cut(s) 151, 182, 342, 359, 489
NlaIV GGNNCC 1 cut(s) 144
NspI RCATGY 2 cut(s) 182, 489
PaeI GCATGC 1 cut(s) 182
PagI TCATGA 1 cut(s) 355
PaqCI CACCTGC 1 cut(s) 41
PctI GAATGC 1 cut(s) 404
PdiI GCCGGC 1 cut(s) 204
PdmI GAANNNNTTC 1 cut(s) 613
PfoI TCCNGGA 1 cut(s) 447
Psp6I CCWGG 1 cut(s) 447
PspGI CCWGG 1 cut(s) 447
PspN4I GGNNCC 1 cut(s) 144
SaqAI TTAA 4 cut(s) 231, 267, 387, 623
ScrFI CCNGG 1 cut(s) 449
SduI GDGCHC 1 cut(s) 147
SetI ASST 5 cut(s) 26, 35, 286, 473, 659
SfaNI GCATC 1 cut(s) 588
SmlI CTYRAG 1 cut(s) 629
SmoI CTYRAG 1 cut(s) 629
SphI GCATGC 1 cut(s) 182
Sse9I AATT 8 cut(s) 67, 74, 137, 457, 507, 609, 647, 699
SsiI CCGC 1 cut(s) 36
SspMI CTAG 1 cut(s) 407
StyD4I CCNGG 1 cut(s) 447
StyI CCWWGG 1 cut(s) 147
TaqI TCGA 2 cut(s) 65, 196
TasI AATT 8 cut(s) 67, 74, 137, 457, 507, 609, 647, 699
Tru1I TTAA 4 cut(s) 231, 267, 387, 623
Tru9I TTAA 4 cut(s) 231, 267, 387, 623
TscAI CASTG 2 cut(s) 189, 304
TspDTI ATGAA 4 cut(s) 344, 492, 549, 708
TspRI CASTG 2 cut(s) 189, 304
XapI RAATTY 1 cut(s) 67
XceI RCATGY 2 cut(s) 182, 489
XmiI GTMKAC 1 cut(s) 382
XmnI GAANNNNTTC 1 cut(s) 613
XspI CTAG 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.