Rh2BG224800

tRNA-dihydrouridine20 synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001304
Physical Location & Seq
Forward (+)
21783264 .. 21788237
4974 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG224800.1

Sequence Viewer

Length: 771 bp
ATGTCCCAAAAAGTCTTCTTCTTCTTCTCGTTACTTTTTCAGTCTTCTCTCTCCGAACACGGTCTCCTTCGTCCTTCGATTCTCACAAGCTCGCCAAATCCCATGTCGAATTCTGAATTAGAGGACAAGATTTTCAGCTTGTTCAAGGATTTTATGGCCGGGATTGCCAAGTTTGAGGAATTGGTGCCCATGGGAAGCACATTTCTCAATGGCTTTCAGCATGCACTGGAGTTTCTTCGACGCCCGGTGATAGATAAAACATCTGAGTTGGTTAAAAATATAATCCAATCTAACGAAACAAAGAGGGTTAAATCATACATTGAAGCTGGATGTATCAACACTGATGATGGCAGGCACAATATAACCAAGTTGCAATCATGCCAACTTGGACTTCATGACCATATATGTAAAGGAAAAGGTATACTTAACGAACTGGAATGCCTACTGGAAGATGTAAGTGGTGCAATGCAAGATAAATCCTTGTTCCAGGATGACAATTTGGGTGAAAGCTTGAATGAACAAGCATGTATCAATGACAAGGAGGAAATTGCGTCGCCTTGTCCCAGAAGAAATGAAGTTACTGATTATGCTGTCCTGATGGCAGTCATTTATCGTATGGTCAAGCAAGAATATATGATGCAGGAAAGAATTGTTTCTGCCTTAAGTCTCAAGTCATCTTCCGGAGAATTAGAGAGCTACTGCCTTATGTGGTCTTTGCGTCCTTACATAGATGAAGAAATTATGGGTCATGCTTGGAAACTTATACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

256

Amino Acids

29.15

Weight (kDa)

5.23

Isoelectric Point (pI)

64.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7795 PF25071 38 - 158 1.3e-54 Domain of unknown function (DUF7795)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 184
AccI GTMKAC 1 cut(s) 421
AccIII TCCGGA 1 cut(s) 680
AcoI YGGCCR 1 cut(s) 156
AcsI RAATTY 1 cut(s) 109
AcyI GRCGYC 1 cut(s) 241
AflII CTTAAG 1 cut(s) 661
AgsI TTSAA 3 cut(s) 145, 323, 514
AjnI CCWGG 1 cut(s) 486
AjuI GAANNNNNNNTTGG 2 cut(s) 375, 407
AloI GAACNNNNNNTCC 2 cut(s) 48, 80
AluBI AGCT 5 cut(s) 90, 138, 326, 510, 696
AluI AGCT 5 cut(s) 90, 138, 326, 510, 696
Alw26I GTCTC 2 cut(s) 68, 671
Aor13HI TCCGGA 1 cut(s) 680
AoxI GGCC 1 cut(s) 156
ApoI RAATTY 1 cut(s) 109
ArsI GACNNNNNNTTYG 2 cut(s) 89, 121
Asp700I GAANNNNTTC 1 cut(s) 652
AsuC2I CCSGG 2 cut(s) 160, 245
AsuHPI GGTGA 2 cut(s) 259, 515
BaeGI GKGCMC 1 cut(s) 189
BanI GGYRCC 1 cut(s) 184
BbsI GAAGAC 2 cut(s) 7, 36
BccI CCATC 2 cut(s) 341, 592
BciT130I CCWGG 1 cut(s) 488
BcnI CCSGG 2 cut(s) 160, 245
BcoDI GTCTC 2 cut(s) 68, 671
BfrI CTTAAG 1 cut(s) 661
Bme1390I CCNGG 3 cut(s) 160, 245, 488
BmiI GGNNCC 1 cut(s) 186
BmrFI CCNGG 3 cut(s) 160, 245, 488
BmsI GCATC 1 cut(s) 627
BpiI GAAGAC 2 cut(s) 7, 36
BpmI CTGGAG 1 cut(s) 248
BpuEI CTTGAG 1 cut(s) 653
BpuMI CCSGG 2 cut(s) 160, 245
BsaHI GRCGYC 1 cut(s) 241
BsaI GGTCTC 1 cut(s) 68
BsaJI CCNNGG 1 cut(s) 189
BsaWI WCCGGW 1 cut(s) 680
BsaXI ACNNNNNCTCC 2 cut(s) 48, 78
Bse1I ACTGG 3 cut(s) 231, 438, 450
Bse3DI GCAATG 1 cut(s) 471
BseAI TCCGGA 1 cut(s) 680
BseBI CCWGG 1 cut(s) 488
BseDI CCNNGG 1 cut(s) 189
BseGI GGATG 2 cut(s) 335, 496
BseMI GCAATG 1 cut(s) 471
BseMII CTCAG 1 cut(s) 255
BseNI ACTGG 3 cut(s) 231, 438, 450
BseSI GKGCMC 1 cut(s) 189
BshFI GGCC 1 cut(s) 158
BshNI GGYRCC 1 cut(s) 184
BsiSI CCGG 3 cut(s) 159, 245, 681
BslFI GGGAC 1 cut(s) 546
BsmAI GTCTC 2 cut(s) 68, 671
BsmFI GGGAC 1 cut(s) 546
BsmI GAATGC 1 cut(s) 443
BsnI GGCC 1 cut(s) 158
Bso31I GGTCTC 1 cut(s) 68
Bsp1286I GDGCHC 1 cut(s) 189
Bsp13I TCCGGA 1 cut(s) 680
Bsp19I CCATGG 1 cut(s) 189
BspANI GGCC 1 cut(s) 158
BspCNI CTCAG 1 cut(s) 256
BspEI TCCGGA 1 cut(s) 680
BspHI TCATGA 1 cut(s) 394
BspLI GGNNCC 1 cut(s) 186
BspT107I GGYRCC 1 cut(s) 184
BspTI CTTAAG 1 cut(s) 661
BspTNI GGTCTC 1 cut(s) 68
BsrDI GCAATG 1 cut(s) 471
BsrI ACTGG 3 cut(s) 231, 438, 450
BssECI CCNNGG 1 cut(s) 189
BssNAI GTATAC 1 cut(s) 422
BssNI GRCGYC 1 cut(s) 241
BssT1I CCWWGG 1 cut(s) 189
Bst1107I GTATAC 1 cut(s) 422
Bst2UI CCWGG 1 cut(s) 488
Bst4CI ACNGT 1 cut(s) 62
BstACI GRCGYC 1 cut(s) 241
BstAFI CTTAAG 1 cut(s) 661
BstC8I GCNNGC 3 cut(s) 92, 222, 353
BstDEI CTNAG 1 cut(s) 264
BstDSI CCRYGG 1 cut(s) 189
BstF5I GGATG 2 cut(s) 335, 496
BstMAI GTCTC 2 cut(s) 68, 671
BstMWI GCNNNNNNNGC 1 cut(s) 164
BstNI CCWGG 1 cut(s) 488
BstNSI RCATGY 2 cut(s) 224, 528
BstSCI CCNGG 3 cut(s) 158, 243, 486
BstSLI GKGCMC 1 cut(s) 189
BstV2I GAAGAC 2 cut(s) 7, 36
BstZ17I GTATAC 1 cut(s) 422
BsuRI GGCC 1 cut(s) 158
BtgI CCRYGG 1 cut(s) 189
BtsCI GGATG 2 cut(s) 335, 496
BtsIMutI CAGTG 3 cut(s) 224, 339, 766
Cac8I GCNNGC 3 cut(s) 92, 222, 353
CciI TCATGA 1 cut(s) 394
CseI GACGC 3 cut(s) 249, 540, 707
CviAII CATG 7 cut(s) 103, 190, 221, 378, 395, 525, 749
CviJI RGCY 7 cut(s) 90, 138, 158, 213, 326, 510, 696
CviKI_1 RGCY 7 cut(s) 90, 138, 158, 213, 326, 510, 696
DdeI CTNAG 1 cut(s) 264
EaeI YGGCCR 1 cut(s) 156
Eco130I CCWWGG 1 cut(s) 189
Eco31I GGTCTC 1 cut(s) 68
EcoRI GAATTC 1 cut(s) 109
EcoRII CCWGG 1 cut(s) 486
EcoT14I CCWWGG 1 cut(s) 189
ErhI CCWWGG 1 cut(s) 189
FaeI CATG 7 cut(s) 106, 193, 224, 381, 398, 528, 752
FalI AAGNNNNNCTT 2 cut(s) 408, 440
FaqI GGGAC 1 cut(s) 546
FatI CATG 7 cut(s) 102, 189, 220, 377, 394, 524, 748
FblI GTMKAC 1 cut(s) 421
FokI GGATG 2 cut(s) 342, 503
GsuI CTGGAG 1 cut(s) 248
HaeIII GGCC 1 cut(s) 158
HapII CCGG 3 cut(s) 159, 245, 681
HgaI GACGC 3 cut(s) 249, 540, 707
Hin1I GRCGYC 1 cut(s) 241
Hin1II CATG 7 cut(s) 106, 193, 224, 381, 398, 528, 752
HindIII AAGCTT 1 cut(s) 508
HinfI GANTC 1 cut(s) 79
HpaII CCGG 3 cut(s) 159, 245, 681
HphI GGTGA 2 cut(s) 259, 515
Hpy166II GTNNAC 1 cut(s) 422
Hpy188I TCNGA 3 cut(s) 55, 115, 265
Hpy188III TCNNGA 3 cut(s) 395, 595, 681
Hpy8I GTNNAC 1 cut(s) 422
Hpy99I CGWCG 2 cut(s) 243, 556
HpyAV CCTTC 2 cut(s) 77, 84
HpyCH4III ACNGT 1 cut(s) 62
HpyCH4V TGCA 5 cut(s) 224, 373, 464, 469, 640
HpyF10VI GCNNNNNNNGC 1 cut(s) 164
HpyF3I CTNAG 1 cut(s) 264
Hsp92I GRCGYC 1 cut(s) 241
Hsp92II CATG 7 cut(s) 106, 193, 224, 381, 398, 528, 752
Kpn2I TCCGGA 1 cut(s) 680
LweI GCATC 1 cut(s) 627
MaeIII GTNAC 2 cut(s) 30, 577
MhlI GDGCHC 1 cut(s) 189
MluCI AATT 8 cut(s) 109, 116, 179, 496, 546, 648, 686, 738
MnlI CCTC 4 cut(s) 115, 169, 297, 535
MroI TCCGGA 1 cut(s) 680
MroXI GAANNNNTTC 1 cut(s) 652
MseI TTAA 4 cut(s) 273, 309, 426, 662
MspCI CTTAAG 1 cut(s) 661
MspI CCGG 3 cut(s) 159, 245, 681
MspR9I CCNGG 3 cut(s) 160, 245, 488
Mva1269I GAATGC 1 cut(s) 443
MvaI CCWGG 1 cut(s) 488
MwoI GCNNNNNNNGC 1 cut(s) 164
NciI CCSGG 2 cut(s) 160, 245
NcoI CCATGG 1 cut(s) 189
NlaIII CATG 7 cut(s) 106, 193, 224, 381, 398, 528, 752
NlaIV GGNNCC 1 cut(s) 186
NspI RCATGY 2 cut(s) 224, 528
PaeI GCATGC 1 cut(s) 224
PagI TCATGA 1 cut(s) 394
PctI GAATGC 1 cut(s) 443
PdmI GAANNNNTTC 1 cut(s) 652
PfeI GAWTC 1 cut(s) 79
PfoI TCCNGGA 1 cut(s) 486
Psp6I CCWGG 1 cut(s) 486
PspGI CCWGG 1 cut(s) 486
PspN4I GGNNCC 1 cut(s) 186
SaqAI TTAA 4 cut(s) 273, 309, 426, 662
ScrFI CCNGG 3 cut(s) 160, 245, 488
SduI GDGCHC 1 cut(s) 189
SetI ASST 6 cut(s) 92, 140, 328, 421, 512, 698
SfaNI GCATC 1 cut(s) 627
SmlI CTYRAG 2 cut(s) 661, 668
SmoI CTYRAG 2 cut(s) 661, 668
SphI GCATGC 1 cut(s) 224
Sse9I AATT 8 cut(s) 109, 116, 179, 496, 546, 648, 686, 738
StyD4I CCNGG 3 cut(s) 158, 243, 486
StyI CCWWGG 1 cut(s) 189
TaaI ACNGT 1 cut(s) 62
TaqI TCGA 3 cut(s) 77, 107, 238
TasI AATT 8 cut(s) 109, 116, 179, 496, 546, 648, 686, 738
TfiI GAWTC 1 cut(s) 79
Tru1I TTAA 4 cut(s) 273, 309, 426, 662
Tru9I TTAA 4 cut(s) 273, 309, 426, 662
TscAI CASTG 2 cut(s) 231, 346
TspDTI ATGAA 4 cut(s) 383, 531, 588, 747
TspRI CASTG 2 cut(s) 231, 346
Vha464I CTTAAG 1 cut(s) 661
XapI RAATTY 1 cut(s) 109
XceI RCATGY 2 cut(s) 224, 528
XmiI GTMKAC 1 cut(s) 421
XmnI GAANNNNTTC 1 cut(s) 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.