RLG00000030262

tRNA-dihydrouridine20 synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001304
Physical Location & Seq
Reverse (-)
58559626 .. 58562074
2449 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030262

Sequence Viewer

Length: 939 bp
ATGTCGAATTCTGAATTAGATGACAAGATTTTCAGCTTGTTCAAGGATTTTATGGCCGGGATTGCCAAGTTTGAGGAATTAGTGCCCATGGGAAGCACATTTCTCAATGGCTTTCAGCATGCACTGGAGTTTCTTCGACGGCCGGCGATAGATAAAACATCTGAGTTGGTTAAAAATATTATCAAATCAAACGAAACAAAGAGGGTTAAATCATACATTGAAGCTGGATGTATCAACACTGATGATGGTAGGCACAATATAACCAAGTTGCAATCATGCCAACTTGGACTTCATGACCATATATGTAAAGGAAAATGTATACTTAACGAACTGGAATGCCTACTGGAAGATGTAAGTGGTGCAATGCAAGATAAATCCTTGTTCCAGGATGACAATTTGGGTGAAAGGTTGAATGAACAAACATGTATCAATGACAAGTGTGGAACACTGGAACTTGCTTCTTCCTTCCAATTCGATGTGAGGAACAATGGTTTAGAAGAAGGTTTATTTCTAATCCTAATAAAAGATGCAGAGAGCTATAGCTATCTTGATGATGCCGTCTCTGCACAGAAGCCACAGACACCCAGAAAGGCCTTCCAAACCTACCATCAAAGCCAACTTTCCAAACCCCAAGGCAAACACCTCCTCTTCCAATCTCAGCCCCGGGCTTTGAAGTGCCCATCTGGCAGTCAGCAAAGAGGCGACGCGGCAAAGCCGGCGCTATCCATGGCCGTCGCCGGACAGGGAACAGTTTGTCGGAGAGGTTGTGCTCTCTCTTGCGGCGCTAGGGTTTCTCCAAAATTTTTTAATGAGTTGGATTGGGCAATGAGTTTCGATGTATTGGGAGTATCCGTATTTATAGAAGCTTTAATTTTCGTGCTTATCCTAATTGTTGGTTCAATTTATGCATGGCGAAAAGGAGCATTGGAATGGTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

313

Amino Acids

34.69

Weight (kDa)

6.11

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7795 PF25071 4 - 124 1.2e-55 Domain of unknown function (DUF7795)
Oxidored_q4 PF00507 273 - 311 2.3e-08 NADH-ubiquinone/plastoquinone oxidoreductase, chain 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 319
AccII CGCG 1 cut(s) 707
AciI CCGC 2 cut(s) 707, 780
AcoI YGGCCR 3 cut(s) 54, 140, 729
AcsI RAATTY 2 cut(s) 7, 800
AflIII ACRYGT 1 cut(s) 422
AgsI TTSAA 5 cut(s) 43, 221, 412, 673, 900
AjnI CCWGG 1 cut(s) 384
AjuI GAANNNNNNNTTGG 2 cut(s) 273, 305
AluBI AGCT 5 cut(s) 36, 224, 537, 543, 866
AluI AGCT 5 cut(s) 36, 224, 537, 543, 866
Alw21I GWGCWC 1 cut(s) 772
Alw26I GTCTC 1 cut(s) 565
Ama87I CYCGRG 1 cut(s) 663
AoxI GGCC 4 cut(s) 54, 140, 591, 729
ApoI RAATTY 2 cut(s) 7, 800
AspLEI GCGC 2 cut(s) 721, 785
AsuC2I CCSGG 3 cut(s) 58, 664, 665
AsuHPI GGTGA 1 cut(s) 413
AvaI CYCGRG 1 cut(s) 663
BaeGI GKGCMC 2 cut(s) 87, 680
Bbv12I GWGCWC 1 cut(s) 772
BccI CCATC 3 cut(s) 239, 615, 688
BceAI ACGGC 3 cut(s) 155, 542, 716
BciT130I CCWGG 1 cut(s) 386
BciVI GTATCC 1 cut(s) 859
BcnI CCSGG 3 cut(s) 58, 664, 665
BcoDI GTCTC 1 cut(s) 565
BfaI CTAG 1 cut(s) 786
BfmI CTRYAG 1 cut(s) 538
BfoI RGCGCY 2 cut(s) 722, 786
BfuI GTATCC 1 cut(s) 859
BglI GCCNNNNNGGC 1 cut(s) 684
BisI GCNGC 2 cut(s) 708, 781
BlsI GCNGC 2 cut(s) 709, 782
Bme1390I CCNGG 4 cut(s) 58, 386, 664, 665
BmeT110I CYCGRG 1 cut(s) 663
BmrFI CCNGG 4 cut(s) 58, 386, 664, 665
BmsI GCATC 2 cut(s) 517, 544
BpmI CTGGAG 1 cut(s) 146
BpuMI CCSGG 3 cut(s) 58, 664, 665
BsaJI CCNNGG 5 cut(s) 87, 631, 662, 663, 726
BsaXI ACNNNNNCTCC 4 cut(s) 751, 781, 837, 867
Bse118I RCCGGY 2 cut(s) 142, 715
Bse1I ACTGG 4 cut(s) 129, 336, 348, 453
Bse3DI GCAATG 2 cut(s) 369, 831
BseBI CCWGG 1 cut(s) 386
BseDI CCNNGG 5 cut(s) 87, 631, 662, 663, 726
BseGI GGATG 2 cut(s) 233, 394
BseMI GCAATG 2 cut(s) 369, 831
BseMII CTCAG 2 cut(s) 153, 671
BseNI ACTGG 4 cut(s) 129, 336, 348, 453
BseRI GAGGAG 1 cut(s) 635
BseSI GKGCMC 2 cut(s) 87, 680
BseX3I CGGCCG 1 cut(s) 140
BsgI GTGCAG 1 cut(s) 549
Bsh1236I CGCG 1 cut(s) 707
Bsh1285I CGRYCG 1 cut(s) 143
BshFI GGCC 4 cut(s) 56, 142, 593, 731
BsiEI CGRYCG 1 cut(s) 143
BsiHKAI GWGCWC 1 cut(s) 772
BsiHKCI CYCGRG 1 cut(s) 663
BsiSI CCGG 5 cut(s) 57, 143, 664, 716, 738
BsmAI GTCTC 1 cut(s) 565
BsmBI CGTCTC 1 cut(s) 565
BsmI GAATGC 1 cut(s) 341
BsnI GGCC 4 cut(s) 56, 142, 593, 731
BsoBI CYCGRG 1 cut(s) 663
Bsp1286I GDGCHC 3 cut(s) 87, 680, 772
Bsp19I CCATGG 2 cut(s) 87, 726
BspACI CCGC 2 cut(s) 707, 780
BspANI GGCC 4 cut(s) 56, 142, 593, 731
BspCNI CTCAG 2 cut(s) 154, 670
BspFNI CGCG 1 cut(s) 707
BspHI TCATGA 1 cut(s) 292
BsrDI GCAATG 2 cut(s) 369, 831
BsrFI RCCGGY 2 cut(s) 142, 715
BsrI ACTGG 4 cut(s) 129, 336, 348, 453
BssAI RCCGGY 2 cut(s) 142, 715
BssECI CCNNGG 5 cut(s) 87, 631, 662, 663, 726
BssNAI GTATAC 1 cut(s) 320
BssT1I CCWWGG 3 cut(s) 87, 631, 726
Bst1107I GTATAC 1 cut(s) 320
Bst2UI CCWGG 1 cut(s) 386
Bst4CI ACNGT 1 cut(s) 751
Bst6I CTCTTC 1 cut(s) 653
BstC8I GCNNGC 3 cut(s) 120, 144, 717
BstDEI CTNAG 3 cut(s) 162, 657, 936
BstDSI CCRYGG 2 cut(s) 87, 726
BstF5I GGATG 2 cut(s) 233, 394
BstFNI CGCG 1 cut(s) 707
BstH2I RGCGCY 2 cut(s) 722, 786
BstHHI GCGC 2 cut(s) 721, 785
BstMAI GTCTC 1 cut(s) 565
BstMCI CGRYCG 1 cut(s) 143
BstMWI GCNNNNNNNGC 4 cut(s) 62, 563, 684, 716
BstNI CCWGG 1 cut(s) 386
BstNSI RCATGY 2 cut(s) 122, 426
BstSCI CCNGG 4 cut(s) 56, 384, 662, 663
BstSFI CTRYAG 1 cut(s) 538
BstSLI GKGCMC 2 cut(s) 87, 680
BstUI CGCG 1 cut(s) 707
BstZ17I GTATAC 1 cut(s) 320
BstZI CGGCCG 1 cut(s) 140
BsuI GTATCC 1 cut(s) 859
BsuRI GGCC 4 cut(s) 56, 142, 593, 731
BtgI CCRYGG 2 cut(s) 87, 726
BtsCI GGATG 2 cut(s) 233, 394
BtsIMutI CAGTG 3 cut(s) 122, 237, 446
Cac8I GCNNGC 3 cut(s) 120, 144, 717
CciI TCATGA 1 cut(s) 292
CfoI GCGC 2 cut(s) 721, 785
Cfr10I RCCGGY 2 cut(s) 142, 715
Cfr9I CCCGGG 1 cut(s) 663
CseI GACGC 1 cut(s) 713
CviAII CATG 7 cut(s) 88, 119, 276, 293, 423, 727, 909
DdeI CTNAG 3 cut(s) 162, 657, 936
EaeI YGGCCR 3 cut(s) 54, 140, 729
EagI CGGCCG 1 cut(s) 140
Eam1104I CTCTTC 1 cut(s) 653
EarI CTCTTC 1 cut(s) 653
EclXI CGGCCG 1 cut(s) 140
Eco130I CCWWGG 3 cut(s) 87, 631, 726
Eco147I AGGCCT 1 cut(s) 593
Eco52I CGGCCG 1 cut(s) 140
Eco88I CYCGRG 1 cut(s) 663
EcoRI GAATTC 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 384
EcoT14I CCWWGG 3 cut(s) 87, 631, 726
EcoT22I ATGCAT 1 cut(s) 910
ErhI CCWWGG 3 cut(s) 87, 631, 726
Esp3I CGTCTC 1 cut(s) 565
FaeI CATG 7 cut(s) 91, 122, 279, 296, 426, 730, 912
FatI CATG 7 cut(s) 87, 118, 275, 292, 422, 726, 908
FblI GTMKAC 1 cut(s) 319
Fnu4HI GCNGC 2 cut(s) 708, 781
FokI GGATG 2 cut(s) 240, 401
Fsp4HI GCNGC 2 cut(s) 708, 781
FspBI CTAG 1 cut(s) 786
GlaI GCGC 2 cut(s) 720, 784
GluI GCNGC 2 cut(s) 708, 781
GsuI CTGGAG 1 cut(s) 146
HaeII RGCGCY 2 cut(s) 722, 786
HaeIII GGCC 4 cut(s) 56, 142, 593, 731
HapII CCGG 5 cut(s) 57, 143, 664, 716, 738
HgaI GACGC 1 cut(s) 713
HhaI GCGC 2 cut(s) 721, 785
Hin1II CATG 7 cut(s) 91, 122, 279, 296, 426, 730, 912
Hin6I GCGC 2 cut(s) 719, 783
HinP1I GCGC 2 cut(s) 719, 783
HindIII AAGCTT 1 cut(s) 864
HpaII CCGG 5 cut(s) 57, 143, 664, 716, 738
HphI GGTGA 1 cut(s) 413
Hpy166II GTNNAC 1 cut(s) 320
Hpy188I TCNGA 3 cut(s) 13, 163, 759
Hpy188III TCNNGA 2 cut(s) 293, 548
Hpy8I GTNNAC 1 cut(s) 320
Hpy99I CGWCG 3 cut(s) 141, 707, 737
HpyAV CCTTC 3 cut(s) 475, 494, 604
HpyCH4III ACNGT 1 cut(s) 751
HpyCH4V TGCA 7 cut(s) 122, 271, 362, 367, 530, 566, 908
HpyF10VI GCNNNNNNNGC 4 cut(s) 62, 563, 684, 716
HpyF3I CTNAG 3 cut(s) 162, 657, 936
Hsp92II CATG 7 cut(s) 91, 122, 279, 296, 426, 730, 912
HspAI GCGC 2 cut(s) 719, 783
KroI GCCGGC 2 cut(s) 142, 715
KroNI GCCGGC 2 cut(s) 144, 717
LmnI GCTCC 1 cut(s) 920
LweI GCATC 2 cut(s) 517, 544
MaeI CTAG 1 cut(s) 786
MboII GAAGA 5 cut(s) 125, 359, 453, 509, 640
MhlI GDGCHC 3 cut(s) 87, 680, 772
MluCI AATT 9 cut(s) 7, 14, 77, 394, 470, 800, 870, 888, 900
MmeI TCCRAC 2 cut(s) 737, 795
MnlI CCTC 7 cut(s) 67, 195, 474, 653, 656, 692, 755
Mph1103I ATGCAT 1 cut(s) 910
MroNI GCCGGC 2 cut(s) 142, 715
MseI TTAA 5 cut(s) 171, 207, 324, 807, 869
MslI CAYNNNNRTG 1 cut(s) 928
MspI CCGG 5 cut(s) 57, 143, 664, 716, 738
MspR9I CCNGG 4 cut(s) 58, 386, 664, 665
Mva1269I GAATGC 1 cut(s) 341
MvaI CCWGG 1 cut(s) 386
MvnI CGCG 1 cut(s) 707
MwoI GCNNNNNNNGC 4 cut(s) 62, 563, 684, 716
NaeI GCCGGC 2 cut(s) 144, 717
NciI CCSGG 3 cut(s) 58, 664, 665
NcoI CCATGG 2 cut(s) 87, 726
NgoMIV GCCGGC 2 cut(s) 142, 715
NlaIII CATG 7 cut(s) 91, 122, 279, 296, 426, 730, 912
NsiI ATGCAT 1 cut(s) 910
NspI RCATGY 2 cut(s) 122, 426
PaeI GCATGC 1 cut(s) 122
PagI TCATGA 1 cut(s) 292
PceI AGGCCT 1 cut(s) 593
PciI ACATGT 1 cut(s) 422
PctI GAATGC 1 cut(s) 341
PdiI GCCGGC 2 cut(s) 144, 717
PfoI TCCNGGA 1 cut(s) 384
PkrI GCNGC 2 cut(s) 709, 782
PscI ACATGT 1 cut(s) 422
Psp6I CCWGG 1 cut(s) 384
PspGI CCWGG 1 cut(s) 384
RseI CAYNNNNRTG 1 cut(s) 928
SaqAI TTAA 5 cut(s) 171, 207, 324, 807, 869
SatI GCNGC 2 cut(s) 708, 781
ScrFI CCNGG 4 cut(s) 58, 386, 664, 665
SduI GDGCHC 3 cut(s) 87, 680, 772
SfaNI GCATC 2 cut(s) 517, 544
SfcI CTRYAG 1 cut(s) 538
SmaI CCCGGG 1 cut(s) 665
SmiMI CAYNNNNRTG 1 cut(s) 928
SphI GCATGC 1 cut(s) 122
Sse9I AATT 9 cut(s) 7, 14, 77, 394, 470, 800, 870, 888, 900
SseBI AGGCCT 1 cut(s) 593
SsiI CCGC 2 cut(s) 707, 780
SspI AATATT 1 cut(s) 178
SspMI CTAG 1 cut(s) 786
StuI AGGCCT 1 cut(s) 593
StyD4I CCNGG 4 cut(s) 56, 384, 662, 663
StyI CCWWGG 3 cut(s) 87, 631, 726
TaaI ACNGT 1 cut(s) 751
TaqI TCGA 4 cut(s) 5, 136, 474, 834
TasI AATT 9 cut(s) 7, 14, 77, 394, 470, 800, 870, 888, 900
TauI GCSGC 2 cut(s) 710, 783
Tru1I TTAA 5 cut(s) 171, 207, 324, 807, 869
Tru9I TTAA 5 cut(s) 171, 207, 324, 807, 869
TscAI CASTG 3 cut(s) 129, 244, 453
TspDTI ATGAA 2 cut(s) 281, 429
TspGWI ACGGA 1 cut(s) 841
TspMI CCCGGG 1 cut(s) 663
TspRI CASTG 3 cut(s) 129, 244, 453
XapI RAATTY 2 cut(s) 7, 800
XceI RCATGY 2 cut(s) 122, 426
XmaI CCCGGG 1 cut(s) 663
XmiI GTMKAC 1 cut(s) 319
XspI CTAG 1 cut(s) 786
Zsp2I ATGCAT 1 cut(s) 910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.