RLG00000030253

tRNA-dihydrouridine20 synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001304
Physical Location & Seq
Forward (+)
58489862 .. 58493021
3160 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030253

Sequence Viewer

Length: 786 bp
ATGAAAGAGGAAACTCTACGGCCTCCAAATCCTAGTCGTTATGACTTGGTTATCTATCGAGGTCTTTGTTGTCGCAACCAGCACCGATCAAGGCTCGACCCACTGAGTTACAGTAGCCTAGGTGGAGAATGGATAGGATTTGGAACTACAAAGGTGTCGGGCGGCGCAGAGGCAACTGGGCTGAAGTTGTTTTGGTCAACCACAACAAAGTTTGGGTGTCCCTTTTCTGGGTGCATTGGTTCATGGGGTTTGAATGTGTATGGGCCCAACCATGGGTCTCTGTATTGGGTATTTCTGAATTATGGGGTTATGCACTTATGTTTGGGTTTCACATCTGGGATTAGGGGGAAGGTATGGTGTTTTAGGGTTCTCTGCACTTTGAAGCCTTCTCTTCATGACTTGATGGGATTGCTACTTCTATTGTCAGGAAAAGGTATACTTAACGAACTGGAATGCCTACTGGAAGATGTAAGTGGTGCAATGCAAGATAAATCCTTGTTCCAAGATGACAATTTGGGTGAAAGGTTGAATGAACAAGCATGTATCAATGACAAGGAGGAAATTGCCCCGCCTTGTCCCAGAAGAAATGAAGTTACTGATTATGCTGTCCTGATGGCAGTCATTTATCGTATGGTCAAGCAAGAATATATGATGCAAGAAAGAATTTTTTCTGCGTTAAGTCTCAAGTCATCTTCCGGAGAATTAGAGAGCTACTGCCTTATGTGGTCTTTGCGTCCTTACATAGATGAAGAAATTATGGGTCATGCTTGGAAACTTATACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

262

Amino Acids

29.5

Weight (kDa)

6.31

Isoelectric Point (pI)

39.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 436
AccIII TCCGGA 1 cut(s) 695
AciI CCGC 2 cut(s) 162, 569
AcsI RAATTY 1 cut(s) 663
AcuI CTGAAG 1 cut(s) 203
AfiI CCNNNNNNNGG 4 cut(s) 227, 228, 272, 273
AgsI TTSAA 3 cut(s) 253, 382, 529
AluBI AGCT 1 cut(s) 711
AluI AGCT 1 cut(s) 711
Alw26I GTCTC 2 cut(s) 282, 686
Aor13HI TCCGGA 1 cut(s) 695
AoxI GGCC 2 cut(s) 20, 263
ApaI GGGCCC 1 cut(s) 267
ApoI RAATTY 1 cut(s) 663
Asp700I GAANNNNTTC 1 cut(s) 667
AspA2I CCTAGG 1 cut(s) 118
AspLEI GCGC 1 cut(s) 167
AspS9I GGNCC 2 cut(s) 263, 264
AsuHPI GGTGA 1 cut(s) 530
AvrII CCTAGG 1 cut(s) 118
BaeGI GKGCMC 1 cut(s) 267
BanII GRGCYC 1 cut(s) 267
BccI CCATC 2 cut(s) 397, 607
BceAI ACGGC 1 cut(s) 35
BcoDI GTCTC 2 cut(s) 282, 686
BfaI CTAG 2 cut(s) 33, 119
BisI GCNGC 1 cut(s) 163
BlnI CCTAGG 1 cut(s) 118
BlsI GCNGC 1 cut(s) 164
BmgT120I GGNCC 2 cut(s) 263, 264
BmiI GGNNCC 1 cut(s) 265
BmrI ACTGGG 1 cut(s) 186
BmsI GCATC 1 cut(s) 642
BmuI ACTGGG 1 cut(s) 186
BpuEI CTTGAG 1 cut(s) 668
BsaI GGTCTC 1 cut(s) 282
BsaJI CCNNGG 2 cut(s) 118, 271
BsaWI WCCGGW 1 cut(s) 695
Bsc4I CCNNNNNNNGG 4 cut(s) 227, 228, 272, 273
Bse1I ACTGG 3 cut(s) 181, 453, 465
Bse3DI GCAATG 1 cut(s) 486
BseAI TCCGGA 1 cut(s) 695
BseDI CCNNGG 2 cut(s) 118, 271
BseLI CCNNNNNNNGG 4 cut(s) 227, 228, 272, 273
BseMI GCAATG 1 cut(s) 486
BseMII CTCAG 1 cut(s) 95
BseNI ACTGG 3 cut(s) 181, 453, 465
BseSI GKGCMC 1 cut(s) 267
BsgI GTGCAG 1 cut(s) 358
BshFI GGCC 2 cut(s) 22, 265
BsiSI CCGG 1 cut(s) 696
BslFI GGGAC 2 cut(s) 204, 561
BslI CCNNNNNNNGG 4 cut(s) 227, 228, 272, 273
BsmAI GTCTC 2 cut(s) 282, 686
BsmFI GGGAC 2 cut(s) 204, 561
BsmI GAATGC 1 cut(s) 458
BsnI GGCC 2 cut(s) 22, 265
Bso31I GGTCTC 1 cut(s) 282
Bsp120I GGGCCC 1 cut(s) 263
Bsp1286I GDGCHC 1 cut(s) 267
Bsp13I TCCGGA 1 cut(s) 695
Bsp143I GATC 1 cut(s) 86
Bsp19I CCATGG 1 cut(s) 271
BspACI CCGC 2 cut(s) 162, 569
BspANI GGCC 2 cut(s) 22, 265
BspCNI CTCAG 1 cut(s) 96
BspEI TCCGGA 1 cut(s) 695
BspHI TCATGA 1 cut(s) 394
BspLI GGNNCC 1 cut(s) 265
BspTNI GGTCTC 1 cut(s) 282
BsrDI GCAATG 1 cut(s) 486
BsrI ACTGG 3 cut(s) 181, 453, 465
BssECI CCNNGG 2 cut(s) 118, 271
BssMI GATC 1 cut(s) 86
BssNAI GTATAC 1 cut(s) 437
BssT1I CCWWGG 2 cut(s) 118, 271
Bst1107I GTATAC 1 cut(s) 437
Bst4CI ACNGT 1 cut(s) 113
Bst6I CTCTTC 1 cut(s) 396
BstDEI CTNAG 1 cut(s) 104
BstDSI CCRYGG 1 cut(s) 271
BstHHI GCGC 1 cut(s) 167
BstKTI GATC 1 cut(s) 89
BstMAI GTCTC 2 cut(s) 282, 686
BstMBI GATC 1 cut(s) 86
BstNSI RCATGY 1 cut(s) 543
BstSLI GKGCMC 1 cut(s) 267
BstZ17I GTATAC 1 cut(s) 437
BsuRI GGCC 2 cut(s) 22, 265
BtgI CCRYGG 1 cut(s) 271
BtsIMutI CAGTG 2 cut(s) 101, 781
CciI TCATGA 1 cut(s) 394
CfoI GCGC 1 cut(s) 167
Cfr13I GGNCC 2 cut(s) 263, 264
CseI GACGC 1 cut(s) 722
CviAII CATG 5 cut(s) 243, 272, 395, 540, 764
CviJI RGCY 7 cut(s) 22, 94, 117, 181, 265, 385, 711
CviKI_1 RGCY 7 cut(s) 22, 94, 117, 181, 265, 385, 711
DdeI CTNAG 1 cut(s) 104
DpnI GATC 1 cut(s) 88
DpnII GATC 1 cut(s) 86
Eam1104I CTCTTC 1 cut(s) 396
EarI CTCTTC 1 cut(s) 396
Eco130I CCWWGG 2 cut(s) 118, 271
Eco24I GRGCYC 1 cut(s) 267
Eco31I GGTCTC 1 cut(s) 282
Eco57I CTGAAG 1 cut(s) 203
EcoT14I CCWWGG 2 cut(s) 118, 271
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 2 cut(s) 118, 271
FaeI CATG 5 cut(s) 246, 275, 398, 543, 767
FalI AAGNNNNNCTT 2 cut(s) 423, 455
FaqI GGGAC 2 cut(s) 204, 561
FatI CATG 5 cut(s) 242, 271, 394, 539, 763
FauI CCCGC 1 cut(s) 576
FblI GTMKAC 1 cut(s) 436
Fnu4HI GCNGC 1 cut(s) 163
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 1 cut(s) 163
FspBI CTAG 2 cut(s) 33, 119
GlaI GCGC 1 cut(s) 166
GluI GCNGC 1 cut(s) 163
HaeIII GGCC 2 cut(s) 22, 265
HapII CCGG 1 cut(s) 696
HgaI GACGC 1 cut(s) 722
HhaI GCGC 1 cut(s) 167
Hin1II CATG 5 cut(s) 246, 275, 398, 543, 767
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HincII GTYRAC 1 cut(s) 198
HindII GTYRAC 1 cut(s) 198
HpaII CCGG 1 cut(s) 696
HphI GGTGA 1 cut(s) 530
Hpy166II GTNNAC 2 cut(s) 198, 437
Hpy188I TCNGA 1 cut(s) 297
Hpy188III TCNNGA 4 cut(s) 395, 426, 610, 696
Hpy8I GTNNAC 2 cut(s) 198, 437
HpyAV CCTTC 2 cut(s) 343, 396
HpyCH4III ACNGT 1 cut(s) 113
HpyCH4V TGCA 6 cut(s) 234, 313, 375, 479, 484, 655
HpyF3I CTNAG 1 cut(s) 104
Hsp92II CATG 5 cut(s) 246, 275, 398, 543, 767
HspAI GCGC 1 cut(s) 165
Kpn2I TCCGGA 1 cut(s) 695
Kzo9I GATC 1 cut(s) 86
LweI GCATC 1 cut(s) 642
MaeI CTAG 2 cut(s) 33, 119
MaeIII GTNAC 2 cut(s) 107, 592
MalI GATC 1 cut(s) 88
MboI GATC 1 cut(s) 86
MboII GAAGA 5 cut(s) 383, 476, 594, 684, 761
MhlI GDGCHC 1 cut(s) 267
MluCI AATT 6 cut(s) 298, 511, 561, 663, 701, 753
MnlI CCTC 4 cut(s) 33, 53, 163, 550
MroI TCCGGA 1 cut(s) 695
MroXI GAANNNNTTC 1 cut(s) 667
MseI TTAA 2 cut(s) 441, 677
MspI CCGG 1 cut(s) 696
Mva1269I GAATGC 1 cut(s) 458
NcoI CCATGG 1 cut(s) 271
NdeII GATC 1 cut(s) 86
NlaIII CATG 5 cut(s) 246, 275, 398, 543, 767
NlaIV GGNNCC 1 cut(s) 265
NspI RCATGY 1 cut(s) 543
PagI TCATGA 1 cut(s) 394
PctI GAATGC 1 cut(s) 458
PdmI GAANNNNTTC 1 cut(s) 667
PkrI GCNGC 1 cut(s) 164
PspN4I GGNNCC 1 cut(s) 265
PspOMI GGGCCC 1 cut(s) 263
PspPI GGNCC 2 cut(s) 263, 264
SaqAI TTAA 2 cut(s) 441, 677
SatI GCNGC 1 cut(s) 163
Sau3AI GATC 1 cut(s) 86
Sau96I GGNCC 2 cut(s) 263, 264
SduI GDGCHC 1 cut(s) 267
SetI ASST 7 cut(s) 64, 124, 156, 354, 436, 527, 713
SfaNI GCATC 1 cut(s) 642
SmlI CTYRAG 1 cut(s) 683
SmoI CTYRAG 1 cut(s) 683
Sse9I AATT 6 cut(s) 298, 511, 561, 663, 701, 753
SsiI CCGC 2 cut(s) 162, 569
SspMI CTAG 2 cut(s) 33, 119
StyI CCWWGG 2 cut(s) 118, 271
TaaI ACNGT 1 cut(s) 113
TaqI TCGA 2 cut(s) 58, 96
TasI AATT 6 cut(s) 298, 511, 561, 663, 701, 753
TauI GCSGC 1 cut(s) 165
Tru1I TTAA 2 cut(s) 441, 677
Tru9I TTAA 2 cut(s) 441, 677
TscAI CASTG 1 cut(s) 108
TspDTI ATGAA 6 cut(s) 17, 231, 383, 546, 603, 762
TspRI CASTG 1 cut(s) 108
XapI RAATTY 1 cut(s) 663
XceI RCATGY 1 cut(s) 543
XmaJI CCTAGG 1 cut(s) 118
XmiI GTMKAC 1 cut(s) 436
XmnI GAANNNNTTC 1 cut(s) 667
XspI CTAG 2 cut(s) 33, 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.