RchiOBHm_Chr2g0109701

tRNA-dihydrouridine20 synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001304
Physical Location & Seq
Forward (+)
21055464 .. 21060763
5300 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48351

Sequence Viewer

Length: 768 bp
ATGTCCCAAAAAGTCTTCTTCTTCTCGTTACTTTTTCAGTCTTCTCTCTCCGAACACGGTCTCCTTCGTCCTTCGATTCTCACAAGCTCGCCAAATCCCATGTCAAATTCTGAATTAGAGGACAAGATTTTCAGCTTGTTCAAGGATTTTATGGCCGGGATTGCCAAGTTTGAAGAATTGGTGCCCATGGGAAGCACATTTCTCAATGGCTTTCAGCATGCACTGGAGTTTCTTCGACGTCCGGCGATAGATAAAACATCTGAGTTGGTTAAAAATATAATCCAATCTAACGAAACAAAGAGGGTTAAATCATACATTGAAGCTGGATGTATCAACACTGATGATGGCAGGCACAATATAACCAAGTTGCAATCATGCCAACTTGGACTTCATGACCATATATGTAAAGGAAAAGGTATACTTAACGAACTGGAATGCCTACTGGAAGATGTAAGTGGTGCAATGCAAGATAAATCCTTGTTCCAGGATGACAATTTGGGTGAAAGGTTGAATGAACAAGCATGTATCAATGACAAGGAGGAAATTGCGTCGCCTTGTCCCAGAAGAAATGAAGTTACTGATTATGCTGTCCTGATGGCAGTCATTTATCGTATGGTCAAGCAAGAATATATGATGCAGGAAAGAATTGTTTCTGCCTTAAGTCTCAAGTCATCTTCCGGAGAATTAGAGAGCTACTGCCTTATGTGGTCTTTGCGTCCTTACATAGATGAAGAAATTATGGGTCATGCTTGGAAACTTATACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

255

Amino Acids

29.04

Weight (kDa)

5.33

Isoelectric Point (pI)

64.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7795 PF25071 37 - 157 6.3e-55 Domain of unknown function (DUF7795)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 241
AccB1I GGYRCC 1 cut(s) 181
AccI GTMKAC 1 cut(s) 418
AccIII TCCGGA 1 cut(s) 677
AcoI YGGCCR 1 cut(s) 153
AcsI RAATTY 1 cut(s) 106
AcyI GRCGYC 1 cut(s) 238
AflII CTTAAG 1 cut(s) 658
AgsI TTSAA 4 cut(s) 142, 173, 320, 511
AjnI CCWGG 1 cut(s) 483
AjuI GAANNNNNNNTTGG 2 cut(s) 372, 404
AloI GAACNNNNNNTCC 2 cut(s) 45, 77
AluBI AGCT 4 cut(s) 87, 135, 323, 693
AluI AGCT 4 cut(s) 87, 135, 323, 693
Alw26I GTCTC 2 cut(s) 65, 668
Aor13HI TCCGGA 1 cut(s) 677
AoxI GGCC 1 cut(s) 153
ApoI RAATTY 1 cut(s) 106
ArsI GACNNNNNNTTYG 2 cut(s) 86, 118
Asp700I GAANNNNTTC 1 cut(s) 649
AsuC2I CCSGG 1 cut(s) 157
AsuHPI GGTGA 1 cut(s) 512
BaeGI GKGCMC 1 cut(s) 186
BanI GGYRCC 1 cut(s) 181
BbsI GAAGAC 2 cut(s) 7, 33
BccI CCATC 2 cut(s) 338, 589
BciT130I CCWGG 1 cut(s) 485
BcnI CCSGG 1 cut(s) 157
BcoDI GTCTC 2 cut(s) 65, 668
BfrI CTTAAG 1 cut(s) 658
Bme1390I CCNGG 2 cut(s) 157, 485
BmiI GGNNCC 1 cut(s) 183
BmrFI CCNGG 2 cut(s) 157, 485
BmsI GCATC 1 cut(s) 624
BpiI GAAGAC 2 cut(s) 7, 33
BpmI CTGGAG 1 cut(s) 245
BpuEI CTTGAG 1 cut(s) 650
BpuMI CCSGG 1 cut(s) 157
BsaHI GRCGYC 1 cut(s) 238
BsaI GGTCTC 1 cut(s) 65
BsaJI CCNNGG 1 cut(s) 186
BsaWI WCCGGW 1 cut(s) 677
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bse1I ACTGG 3 cut(s) 228, 435, 447
Bse3DI GCAATG 1 cut(s) 468
BseAI TCCGGA 1 cut(s) 677
BseBI CCWGG 1 cut(s) 485
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 2 cut(s) 332, 493
BseMI GCAATG 1 cut(s) 468
BseMII CTCAG 1 cut(s) 252
BseNI ACTGG 3 cut(s) 228, 435, 447
BseSI GKGCMC 1 cut(s) 186
BshFI GGCC 1 cut(s) 155
BshNI GGYRCC 1 cut(s) 181
BsiSI CCGG 3 cut(s) 156, 242, 678
BslFI GGGAC 1 cut(s) 543
BsmAI GTCTC 2 cut(s) 65, 668
BsmFI GGGAC 1 cut(s) 543
BsmI GAATGC 1 cut(s) 440
BsnI GGCC 1 cut(s) 155
Bso31I GGTCTC 1 cut(s) 65
Bsp1286I GDGCHC 1 cut(s) 186
Bsp13I TCCGGA 1 cut(s) 677
Bsp19I CCATGG 1 cut(s) 186
BspANI GGCC 1 cut(s) 155
BspCNI CTCAG 1 cut(s) 253
BspEI TCCGGA 1 cut(s) 677
BspHI TCATGA 1 cut(s) 391
BspLI GGNNCC 1 cut(s) 183
BspT107I GGYRCC 1 cut(s) 181
BspTI CTTAAG 1 cut(s) 658
BspTNI GGTCTC 1 cut(s) 65
BsrDI GCAATG 1 cut(s) 468
BsrI ACTGG 3 cut(s) 228, 435, 447
BssECI CCNNGG 1 cut(s) 186
BssNAI GTATAC 1 cut(s) 419
BssNI GRCGYC 1 cut(s) 238
BssT1I CCWWGG 1 cut(s) 186
Bst1107I GTATAC 1 cut(s) 419
Bst2UI CCWGG 1 cut(s) 485
Bst4CI ACNGT 1 cut(s) 59
BstACI GRCGYC 1 cut(s) 238
BstAFI CTTAAG 1 cut(s) 658
BstC8I GCNNGC 3 cut(s) 89, 219, 350
BstDEI CTNAG 1 cut(s) 261
BstDSI CCRYGG 1 cut(s) 186
BstF5I GGATG 2 cut(s) 332, 493
BstMAI GTCTC 2 cut(s) 65, 668
BstMWI GCNNNNNNNGC 1 cut(s) 161
BstNI CCWGG 1 cut(s) 485
BstNSI RCATGY 2 cut(s) 221, 525
BstSCI CCNGG 2 cut(s) 155, 483
BstSLI GKGCMC 1 cut(s) 186
BstV2I GAAGAC 2 cut(s) 7, 33
BstZ17I GTATAC 1 cut(s) 419
BsuRI GGCC 1 cut(s) 155
BtgI CCRYGG 1 cut(s) 186
BtsCI GGATG 2 cut(s) 332, 493
BtsIMutI CAGTG 3 cut(s) 221, 336, 763
Cac8I GCNNGC 3 cut(s) 89, 219, 350
CciI TCATGA 1 cut(s) 391
CseI GACGC 2 cut(s) 537, 704
CviAII CATG 7 cut(s) 100, 187, 218, 375, 392, 522, 746
CviJI RGCY 6 cut(s) 87, 135, 155, 210, 323, 693
CviKI_1 RGCY 6 cut(s) 87, 135, 155, 210, 323, 693
DdeI CTNAG 1 cut(s) 261
EaeI YGGCCR 1 cut(s) 153
Eco130I CCWWGG 1 cut(s) 186
Eco31I GGTCTC 1 cut(s) 65
EcoRII CCWGG 1 cut(s) 483
EcoT14I CCWWGG 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 7 cut(s) 103, 190, 221, 378, 395, 525, 749
FalI AAGNNNNNCTT 2 cut(s) 405, 437
FaqI GGGAC 1 cut(s) 543
FatI CATG 7 cut(s) 99, 186, 217, 374, 391, 521, 745
FblI GTMKAC 1 cut(s) 418
FokI GGATG 2 cut(s) 339, 500
GsuI CTGGAG 1 cut(s) 245
HaeIII GGCC 1 cut(s) 155
HapII CCGG 3 cut(s) 156, 242, 678
HgaI GACGC 2 cut(s) 537, 704
Hin1I GRCGYC 1 cut(s) 238
Hin1II CATG 7 cut(s) 103, 190, 221, 378, 395, 525, 749
HinfI GANTC 1 cut(s) 76
HpaII CCGG 3 cut(s) 156, 242, 678
HphI GGTGA 1 cut(s) 512
Hpy166II GTNNAC 1 cut(s) 419
Hpy188I TCNGA 3 cut(s) 52, 112, 262
Hpy188III TCNNGA 3 cut(s) 392, 592, 678
Hpy8I GTNNAC 1 cut(s) 419
Hpy99I CGWCG 2 cut(s) 240, 553
HpyAV CCTTC 2 cut(s) 74, 81
HpyCH4III ACNGT 1 cut(s) 59
HpyCH4IV ACGT 1 cut(s) 238
HpyCH4V TGCA 5 cut(s) 221, 370, 461, 466, 637
HpyF10VI GCNNNNNNNGC 1 cut(s) 161
HpyF3I CTNAG 1 cut(s) 261
HpySE526I ACGT 1 cut(s) 238
Hsp92I GRCGYC 1 cut(s) 238
Hsp92II CATG 7 cut(s) 103, 190, 221, 378, 395, 525, 749
Kpn2I TCCGGA 1 cut(s) 677
LweI GCATC 1 cut(s) 624
MaeII ACGT 1 cut(s) 238
MaeIII GTNAC 2 cut(s) 27, 574
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 8 cut(s) 106, 113, 176, 493, 543, 645, 683, 735
MnlI CCTC 3 cut(s) 112, 294, 532
MroI TCCGGA 1 cut(s) 677
MroXI GAANNNNTTC 1 cut(s) 649
MseI TTAA 4 cut(s) 270, 306, 423, 659
MspCI CTTAAG 1 cut(s) 658
MspI CCGG 3 cut(s) 156, 242, 678
MspR9I CCNGG 2 cut(s) 157, 485
Mva1269I GAATGC 1 cut(s) 440
MvaI CCWGG 1 cut(s) 485
MwoI GCNNNNNNNGC 1 cut(s) 161
NciI CCSGG 1 cut(s) 157
NcoI CCATGG 1 cut(s) 186
NlaIII CATG 7 cut(s) 103, 190, 221, 378, 395, 525, 749
NlaIV GGNNCC 1 cut(s) 183
NspI RCATGY 2 cut(s) 221, 525
PaeI GCATGC 1 cut(s) 221
PagI TCATGA 1 cut(s) 391
PctI GAATGC 1 cut(s) 440
PdmI GAANNNNTTC 1 cut(s) 649
PfeI GAWTC 1 cut(s) 76
PfoI TCCNGGA 1 cut(s) 483
Psp6I CCWGG 1 cut(s) 483
PspGI CCWGG 1 cut(s) 483
PspN4I GGNNCC 1 cut(s) 183
SaqAI TTAA 4 cut(s) 270, 306, 423, 659
ScrFI CCNGG 2 cut(s) 157, 485
SduI GDGCHC 1 cut(s) 186
SetI ASST 7 cut(s) 89, 137, 241, 325, 418, 509, 695
SfaNI GCATC 1 cut(s) 624
SmlI CTYRAG 2 cut(s) 658, 665
SmoI CTYRAG 2 cut(s) 658, 665
SphI GCATGC 1 cut(s) 221
Sse9I AATT 8 cut(s) 106, 113, 176, 493, 543, 645, 683, 735
StyD4I CCNGG 2 cut(s) 155, 483
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 1 cut(s) 59
TaiI ACGT 1 cut(s) 241
TaqI TCGA 2 cut(s) 74, 235
TasI AATT 8 cut(s) 106, 113, 176, 493, 543, 645, 683, 735
TfiI GAWTC 1 cut(s) 76
Tru1I TTAA 4 cut(s) 270, 306, 423, 659
Tru9I TTAA 4 cut(s) 270, 306, 423, 659
TscAI CASTG 2 cut(s) 228, 343
TspDTI ATGAA 4 cut(s) 380, 528, 585, 744
TspRI CASTG 2 cut(s) 228, 343
Vha464I CTTAAG 1 cut(s) 658
XapI RAATTY 1 cut(s) 106
XceI RCATGY 2 cut(s) 221, 525
XmiI GTMKAC 1 cut(s) 418
XmnI GAANNNNTTC 1 cut(s) 649
ZraI GACGTC 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.