Rw0G009640

tRNA-dihydrouridine20 synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00382
OG0001304
Physical Location & Seq
Forward (+)
20140 .. 23465
3326 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G009640.1

Sequence Viewer

Length: 723 bp
ATGGTGCATGTAGAAGCATTGACTGCTTTGGCATCAGTTGTCGTTTCATTTCCGATGTCGAATTCTGAATTAGAGGACAAGATTTTCAGCTTGTTCATGGATTTTATGGCCGGGATTGCCAAGTTTGAGGAATTGGTGCCCATGGGAAGCACATTTCTCAATGGCTTTCAGCTTGCACTGGAGTTTCTTCGACGGCCGGCGATAGATAAAACATCTGAGTTGGTTAAAAATATAATCCAATCAAACGAAACAAAGAGGGTTAAATCATACATTGAAGCTGGATGTATCAACACTGATGATGGCAGGCACAATATAACCAAGTTGCAATCATGCCAGCTTGGACTTCATGACCATATATGTAAAGGAAAAGGTATACTTAACGAACTGGAATGCCTACTGGAAGATGTAAGTGGTGCAATGCAAGATAAATCCTTGTTCCAGGATGACAATTTGGGTGAAAGGTTGAATGAACAAGCATGTATCAATGACAAGGAGGAAATTGCCTCGCCTTGTCCCAGAAGAAATGAAGTTACTGATTATGCTGTCCTGATGGCAGTCATTTATCGTATGGTCAAGCAAGAATATATGATGCAGGAAAGAATTGTTTCTGCATTAAGTCTCAAGTCATCTTCCGGAGAATTAGAGAGCTACTGCCTTATGTGGTCTTTGCGTCCTTACATAGATGAAGAAATTATGGGTCATGCTTGGAAACTTATACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

240

Amino Acids

27.19

Weight (kDa)

4.99

Isoelectric Point (pI)

58.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7795 PF25071 22 - 142 7e-54 Domain of unknown function (DUF7795)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 136
AccI GTMKAC 1 cut(s) 373
AccIII TCCGGA 1 cut(s) 632
AcoI YGGCCR 2 cut(s) 108, 194
AcsI RAATTY 1 cut(s) 61
AgsI TTSAA 2 cut(s) 275, 466
AjnI CCWGG 1 cut(s) 438
AluBI AGCT 5 cut(s) 90, 172, 278, 337, 648
AluI AGCT 5 cut(s) 90, 172, 278, 337, 648
Alw26I GTCTC 1 cut(s) 623
Aor13HI TCCGGA 1 cut(s) 632
AoxI GGCC 2 cut(s) 108, 194
ApoI RAATTY 1 cut(s) 61
Asp700I GAANNNNTTC 1 cut(s) 604
AsuC2I CCSGG 1 cut(s) 112
AsuHPI GGTGA 1 cut(s) 467
BaeGI GKGCMC 1 cut(s) 141
BanI GGYRCC 1 cut(s) 136
BccI CCATC 2 cut(s) 293, 544
BceAI ACGGC 1 cut(s) 209
BciT130I CCWGG 1 cut(s) 440
BcnI CCSGG 1 cut(s) 112
BcoDI GTCTC 1 cut(s) 623
Bme1390I CCNGG 2 cut(s) 112, 440
BmiI GGNNCC 1 cut(s) 138
BmrFI CCNGG 2 cut(s) 112, 440
BmsI GCATC 2 cut(s) 41, 579
BpmI CTGGAG 1 cut(s) 200
BpuEI CTTGAG 1 cut(s) 605
BpuMI CCSGG 1 cut(s) 112
BsaJI CCNNGG 1 cut(s) 141
BsaWI WCCGGW 1 cut(s) 632
Bse118I RCCGGY 1 cut(s) 196
Bse1I ACTGG 3 cut(s) 183, 390, 402
Bse3DI GCAATG 1 cut(s) 423
BseAI TCCGGA 1 cut(s) 632
BseBI CCWGG 1 cut(s) 440
BseDI CCNNGG 1 cut(s) 141
BseGI GGATG 2 cut(s) 287, 448
BseMI GCAATG 1 cut(s) 423
BseMII CTCAG 1 cut(s) 207
BseNI ACTGG 3 cut(s) 183, 390, 402
BseSI GKGCMC 1 cut(s) 141
BseX3I CGGCCG 1 cut(s) 194
Bsh1285I CGRYCG 1 cut(s) 197
BshFI GGCC 2 cut(s) 110, 196
BshNI GGYRCC 1 cut(s) 136
BsiEI CGRYCG 1 cut(s) 197
BsiSI CCGG 3 cut(s) 111, 197, 633
BslFI GGGAC 1 cut(s) 498
BsmAI GTCTC 1 cut(s) 623
BsmFI GGGAC 1 cut(s) 498
BsmI GAATGC 1 cut(s) 395
BsnI GGCC 2 cut(s) 110, 196
Bsp1286I GDGCHC 1 cut(s) 141
Bsp13I TCCGGA 1 cut(s) 632
Bsp19I CCATGG 1 cut(s) 141
BspANI GGCC 2 cut(s) 110, 196
BspCNI CTCAG 1 cut(s) 208
BspEI TCCGGA 1 cut(s) 632
BspHI TCATGA 1 cut(s) 346
BspLI GGNNCC 1 cut(s) 138
BspT107I GGYRCC 1 cut(s) 136
BsrDI GCAATG 1 cut(s) 423
BsrFI RCCGGY 1 cut(s) 196
BsrI ACTGG 3 cut(s) 183, 390, 402
BssAI RCCGGY 1 cut(s) 196
BssECI CCNNGG 1 cut(s) 141
BssNAI GTATAC 1 cut(s) 374
BssT1I CCWWGG 1 cut(s) 141
Bst1107I GTATAC 1 cut(s) 374
Bst2UI CCWGG 1 cut(s) 440
BstAPI GCANNNNNTGC 1 cut(s) 23
BstC8I GCNNGC 4 cut(s) 174, 198, 305, 335
BstDEI CTNAG 1 cut(s) 216
BstDSI CCRYGG 1 cut(s) 141
BstF5I GGATG 2 cut(s) 287, 448
BstMAI GTCTC 1 cut(s) 623
BstMCI CGRYCG 1 cut(s) 197
BstMWI GCNNNNNNNGC 2 cut(s) 23, 116
BstNI CCWGG 1 cut(s) 440
BstNSI RCATGY 2 cut(s) 11, 480
BstSCI CCNGG 2 cut(s) 110, 438
BstSLI GKGCMC 1 cut(s) 141
BstZ17I GTATAC 1 cut(s) 374
BstZI CGGCCG 1 cut(s) 194
BsuRI GGCC 2 cut(s) 110, 196
BtgI CCRYGG 1 cut(s) 141
BtsCI GGATG 2 cut(s) 287, 448
BtsIMutI CAGTG 3 cut(s) 176, 291, 718
Cac8I GCNNGC 4 cut(s) 174, 198, 305, 335
CciI TCATGA 1 cut(s) 346
Cfr10I RCCGGY 1 cut(s) 196
CseI GACGC 1 cut(s) 659
CviAII CATG 7 cut(s) 8, 97, 142, 330, 347, 477, 701
CviJI RGCY 8 cut(s) 90, 110, 165, 172, 196, 278, 337, 648
CviKI_1 RGCY 8 cut(s) 90, 110, 165, 172, 196, 278, 337, 648
DdeI CTNAG 1 cut(s) 216
EaeI YGGCCR 2 cut(s) 108, 194
EagI CGGCCG 1 cut(s) 194
EclXI CGGCCG 1 cut(s) 194
Eco130I CCWWGG 1 cut(s) 141
Eco52I CGGCCG 1 cut(s) 194
EcoRI GAATTC 1 cut(s) 61
EcoRII CCWGG 1 cut(s) 438
EcoT14I CCWWGG 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 141
FaeI CATG 7 cut(s) 11, 100, 145, 333, 350, 480, 704
FalI AAGNNNNNCTT 2 cut(s) 360, 392
FaqI GGGAC 1 cut(s) 498
FatI CATG 7 cut(s) 7, 96, 141, 329, 346, 476, 700
FblI GTMKAC 1 cut(s) 373
FokI GGATG 2 cut(s) 294, 455
GsuI CTGGAG 1 cut(s) 200
HaeIII GGCC 2 cut(s) 110, 196
HapII CCGG 3 cut(s) 111, 197, 633
HgaI GACGC 1 cut(s) 659
Hin1II CATG 7 cut(s) 11, 100, 145, 333, 350, 480, 704
HpaII CCGG 3 cut(s) 111, 197, 633
HphI GGTGA 1 cut(s) 467
Hpy166II GTNNAC 1 cut(s) 374
Hpy188I TCNGA 3 cut(s) 54, 67, 217
Hpy188III TCNNGA 3 cut(s) 347, 547, 633
Hpy8I GTNNAC 1 cut(s) 374
Hpy99I CGWCG 1 cut(s) 195
HpyCH4V TGCA 7 cut(s) 7, 176, 325, 416, 421, 592, 611
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 116
HpyF3I CTNAG 1 cut(s) 216
Hsp92II CATG 7 cut(s) 11, 100, 145, 333, 350, 480, 704
Kpn2I TCCGGA 1 cut(s) 632
KroI GCCGGC 1 cut(s) 196
KroNI GCCGGC 1 cut(s) 198
LweI GCATC 2 cut(s) 41, 579
MaeIII GTNAC 1 cut(s) 529
MboII GAAGA 5 cut(s) 179, 413, 531, 621, 698
MhlI GDGCHC 1 cut(s) 141
MluCI AATT 8 cut(s) 61, 68, 131, 448, 498, 600, 638, 690
MnlI CCTC 5 cut(s) 67, 121, 249, 487, 514
MroI TCCGGA 1 cut(s) 632
MroNI GCCGGC 1 cut(s) 196
MroXI GAANNNNTTC 1 cut(s) 604
MseI TTAA 4 cut(s) 225, 261, 378, 614
MspI CCGG 3 cut(s) 111, 197, 633
MspR9I CCNGG 2 cut(s) 112, 440
Mva1269I GAATGC 1 cut(s) 395
MvaI CCWGG 1 cut(s) 440
MwoI GCNNNNNNNGC 2 cut(s) 23, 116
NaeI GCCGGC 1 cut(s) 198
NciI CCSGG 1 cut(s) 112
NcoI CCATGG 1 cut(s) 141
NgoMIV GCCGGC 1 cut(s) 196
NlaIII CATG 7 cut(s) 11, 100, 145, 333, 350, 480, 704
NlaIV GGNNCC 1 cut(s) 138
NspI RCATGY 2 cut(s) 11, 480
PagI TCATGA 1 cut(s) 346
PctI GAATGC 1 cut(s) 395
PdiI GCCGGC 1 cut(s) 198
PdmI GAANNNNTTC 1 cut(s) 604
PfoI TCCNGGA 1 cut(s) 438
Psp6I CCWGG 1 cut(s) 438
PspGI CCWGG 1 cut(s) 438
PspN4I GGNNCC 1 cut(s) 138
SaqAI TTAA 4 cut(s) 225, 261, 378, 614
ScrFI CCNGG 2 cut(s) 112, 440
SduI GDGCHC 1 cut(s) 141
SetI ASST 7 cut(s) 92, 174, 280, 339, 373, 464, 650
SfaNI GCATC 2 cut(s) 41, 579
SmlI CTYRAG 1 cut(s) 620
SmoI CTYRAG 1 cut(s) 620
Sse9I AATT 8 cut(s) 61, 68, 131, 448, 498, 600, 638, 690
StyD4I CCNGG 2 cut(s) 110, 438
StyI CCWWGG 1 cut(s) 141
TaqI TCGA 2 cut(s) 59, 190
TasI AATT 8 cut(s) 61, 68, 131, 448, 498, 600, 638, 690
Tru1I TTAA 4 cut(s) 225, 261, 378, 614
Tru9I TTAA 4 cut(s) 225, 261, 378, 614
TscAI CASTG 2 cut(s) 183, 298
TspDTI ATGAA 6 cut(s) 36, 85, 335, 483, 540, 699
TspRI CASTG 2 cut(s) 183, 298
XapI RAATTY 1 cut(s) 61
XceI RCATGY 2 cut(s) 11, 480
XmiI GTMKAC 1 cut(s) 373
XmnI GAANNNNTTC 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.