Rh2CG216700

tRNA-dihydrouridine20 synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0001304
Physical Location & Seq
Forward (+)
21299879 .. 21305157
5279 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG216700.1

Sequence Viewer

Length: 669 bp
ATGTCAAATTCTGAATTAGAGGACAAGATTTTCAGCTTGTTCAAGGATTTTATGGCCGGGATTGCCAAGTTTGAAGAATTGGTGCCCATGGGAAGCACATTTCTCAATGGCTTTCAGCATGCACTGGAGTTTCTTCGACGTCCGGCGATAGATAAAACATCTGAGTTGGTTAAAAATATAATCCAATCTAACGAAACAAAGAGGGTTAAATCATACATTGAAGCTGGATGTATCAACACTGATGATGGCAGGCACAATATAACCAAGTTGCAATCATGCCAACTTGGACTTCATGACCATATATGTAAAGGAAAAGGTATACTTAACGAACTGGAATGCCTACTGGAAGATGTAAGTGGTGCAATGCAAGATAAATCCTTGTTCCAGGATGACAATTTGGGTGAAAGGTTGAATGAACAAGCATGTATCAATGACAAGGAGGAAATTGCGTCGCCTTGTCCCAGAAGAAATGAAGTTACTGATTATGCTGTCCTGATGGCAGTCATTTATCGTATGGTCAAGCAAGAATATATGATGCAGGAAAGAATTGTTTCTGCCTTAAGTCTCAAGTCATCTTCCGGAGAATTAGAGAGCTACTGCCTTATGTGGTCTTTGCGTCCTTACATAGATGAAGAAATTATGGGTCATGCTTGGAAACTTATACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

222

Amino Acids

25.36

Weight (kDa)

5.14

Isoelectric Point (pI)

61.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7795 PF25071 4 - 124 4.4e-55 Domain of unknown function (DUF7795)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 142
AccB1I GGYRCC 1 cut(s) 82
AccI GTMKAC 1 cut(s) 319
AccIII TCCGGA 1 cut(s) 578
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 1 cut(s) 7
AcyI GRCGYC 1 cut(s) 139
AflII CTTAAG 1 cut(s) 559
AgsI TTSAA 4 cut(s) 43, 74, 221, 412
AjnI CCWGG 1 cut(s) 384
AjuI GAANNNNNNNTTGG 2 cut(s) 273, 305
AluBI AGCT 3 cut(s) 36, 224, 594
AluI AGCT 3 cut(s) 36, 224, 594
Alw26I GTCTC 1 cut(s) 569
Aor13HI TCCGGA 1 cut(s) 578
AoxI GGCC 1 cut(s) 54
ApoI RAATTY 1 cut(s) 7
Asp700I GAANNNNTTC 1 cut(s) 550
AsuC2I CCSGG 1 cut(s) 58
AsuHPI GGTGA 1 cut(s) 413
BaeGI GKGCMC 1 cut(s) 87
BanI GGYRCC 1 cut(s) 82
BccI CCATC 2 cut(s) 239, 490
BciT130I CCWGG 1 cut(s) 386
BcnI CCSGG 1 cut(s) 58
BcoDI GTCTC 1 cut(s) 569
BfrI CTTAAG 1 cut(s) 559
Bme1390I CCNGG 2 cut(s) 58, 386
BmiI GGNNCC 1 cut(s) 84
BmrFI CCNGG 2 cut(s) 58, 386
BmsI GCATC 1 cut(s) 525
BpmI CTGGAG 1 cut(s) 146
BpuEI CTTGAG 1 cut(s) 551
BpuMI CCSGG 1 cut(s) 58
BsaHI GRCGYC 1 cut(s) 139
BsaJI CCNNGG 1 cut(s) 87
BsaWI WCCGGW 1 cut(s) 578
Bse1I ACTGG 3 cut(s) 129, 336, 348
Bse3DI GCAATG 1 cut(s) 369
BseAI TCCGGA 1 cut(s) 578
BseBI CCWGG 1 cut(s) 386
BseDI CCNNGG 1 cut(s) 87
BseGI GGATG 2 cut(s) 233, 394
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 1 cut(s) 153
BseNI ACTGG 3 cut(s) 129, 336, 348
BseSI GKGCMC 1 cut(s) 87
BshFI GGCC 1 cut(s) 56
BshNI GGYRCC 1 cut(s) 82
BsiSI CCGG 3 cut(s) 57, 143, 579
BslFI GGGAC 1 cut(s) 444
BsmAI GTCTC 1 cut(s) 569
BsmFI GGGAC 1 cut(s) 444
BsmI GAATGC 1 cut(s) 341
BsnI GGCC 1 cut(s) 56
Bsp1286I GDGCHC 1 cut(s) 87
Bsp13I TCCGGA 1 cut(s) 578
Bsp19I CCATGG 1 cut(s) 87
BspANI GGCC 1 cut(s) 56
BspCNI CTCAG 1 cut(s) 154
BspEI TCCGGA 1 cut(s) 578
BspHI TCATGA 1 cut(s) 292
BspLI GGNNCC 1 cut(s) 84
BspT107I GGYRCC 1 cut(s) 82
BspTI CTTAAG 1 cut(s) 559
BsrDI GCAATG 1 cut(s) 369
BsrI ACTGG 3 cut(s) 129, 336, 348
BssECI CCNNGG 1 cut(s) 87
BssNAI GTATAC 1 cut(s) 320
BssNI GRCGYC 1 cut(s) 139
BssT1I CCWWGG 1 cut(s) 87
Bst1107I GTATAC 1 cut(s) 320
Bst2UI CCWGG 1 cut(s) 386
BstACI GRCGYC 1 cut(s) 139
BstAFI CTTAAG 1 cut(s) 559
BstC8I GCNNGC 2 cut(s) 120, 251
BstDEI CTNAG 1 cut(s) 162
BstDSI CCRYGG 1 cut(s) 87
BstF5I GGATG 2 cut(s) 233, 394
BstMAI GTCTC 1 cut(s) 569
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstNI CCWGG 1 cut(s) 386
BstNSI RCATGY 2 cut(s) 122, 426
BstSCI CCNGG 2 cut(s) 56, 384
BstSLI GKGCMC 1 cut(s) 87
BstZ17I GTATAC 1 cut(s) 320
BsuRI GGCC 1 cut(s) 56
BtgI CCRYGG 1 cut(s) 87
BtsCI GGATG 2 cut(s) 233, 394
BtsIMutI CAGTG 3 cut(s) 122, 237, 664
Cac8I GCNNGC 2 cut(s) 120, 251
CciI TCATGA 1 cut(s) 292
CseI GACGC 2 cut(s) 438, 605
CviAII CATG 6 cut(s) 88, 119, 276, 293, 423, 647
CviJI RGCY 5 cut(s) 36, 56, 111, 224, 594
CviKI_1 RGCY 5 cut(s) 36, 56, 111, 224, 594
DdeI CTNAG 1 cut(s) 162
EaeI YGGCCR 1 cut(s) 54
Eco130I CCWWGG 1 cut(s) 87
EcoRII CCWGG 1 cut(s) 384
EcoT14I CCWWGG 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 6 cut(s) 91, 122, 279, 296, 426, 650
FalI AAGNNNNNCTT 2 cut(s) 306, 338
FaqI GGGAC 1 cut(s) 444
FatI CATG 6 cut(s) 87, 118, 275, 292, 422, 646
FblI GTMKAC 1 cut(s) 319
FokI GGATG 2 cut(s) 240, 401
GsuI CTGGAG 1 cut(s) 146
HaeIII GGCC 1 cut(s) 56
HapII CCGG 3 cut(s) 57, 143, 579
HgaI GACGC 2 cut(s) 438, 605
Hin1I GRCGYC 1 cut(s) 139
Hin1II CATG 6 cut(s) 91, 122, 279, 296, 426, 650
HpaII CCGG 3 cut(s) 57, 143, 579
HphI GGTGA 1 cut(s) 413
Hpy166II GTNNAC 1 cut(s) 320
Hpy188I TCNGA 2 cut(s) 13, 163
Hpy188III TCNNGA 3 cut(s) 293, 493, 579
Hpy8I GTNNAC 1 cut(s) 320
Hpy99I CGWCG 2 cut(s) 141, 454
HpyCH4IV ACGT 1 cut(s) 139
HpyCH4V TGCA 5 cut(s) 122, 271, 362, 367, 538
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpyF3I CTNAG 1 cut(s) 162
HpySE526I ACGT 1 cut(s) 139
Hsp92I GRCGYC 1 cut(s) 139
Hsp92II CATG 6 cut(s) 91, 122, 279, 296, 426, 650
Kpn2I TCCGGA 1 cut(s) 578
LweI GCATC 1 cut(s) 525
MaeII ACGT 1 cut(s) 139
MaeIII GTNAC 1 cut(s) 475
MboII GAAGA 6 cut(s) 86, 125, 359, 477, 567, 644
MhlI GDGCHC 1 cut(s) 87
MluCI AATT 8 cut(s) 7, 14, 77, 394, 444, 546, 584, 636
MnlI CCTC 3 cut(s) 13, 195, 433
MroI TCCGGA 1 cut(s) 578
MroXI GAANNNNTTC 1 cut(s) 550
MseI TTAA 4 cut(s) 171, 207, 324, 560
MspCI CTTAAG 1 cut(s) 559
MspI CCGG 3 cut(s) 57, 143, 579
MspR9I CCNGG 2 cut(s) 58, 386
Mva1269I GAATGC 1 cut(s) 341
MvaI CCWGG 1 cut(s) 386
MwoI GCNNNNNNNGC 1 cut(s) 62
NciI CCSGG 1 cut(s) 58
NcoI CCATGG 1 cut(s) 87
NlaIII CATG 6 cut(s) 91, 122, 279, 296, 426, 650
NlaIV GGNNCC 1 cut(s) 84
NspI RCATGY 2 cut(s) 122, 426
PaeI GCATGC 1 cut(s) 122
PagI TCATGA 1 cut(s) 292
PctI GAATGC 1 cut(s) 341
PdmI GAANNNNTTC 1 cut(s) 550
PfoI TCCNGGA 1 cut(s) 384
Psp6I CCWGG 1 cut(s) 384
PspGI CCWGG 1 cut(s) 384
PspN4I GGNNCC 1 cut(s) 84
SaqAI TTAA 4 cut(s) 171, 207, 324, 560
ScrFI CCNGG 2 cut(s) 58, 386
SduI GDGCHC 1 cut(s) 87
SetI ASST 6 cut(s) 38, 142, 226, 319, 410, 596
SfaNI GCATC 1 cut(s) 525
SmlI CTYRAG 2 cut(s) 559, 566
SmoI CTYRAG 2 cut(s) 559, 566
SphI GCATGC 1 cut(s) 122
Sse9I AATT 8 cut(s) 7, 14, 77, 394, 444, 546, 584, 636
StyD4I CCNGG 2 cut(s) 56, 384
StyI CCWWGG 1 cut(s) 87
TaiI ACGT 1 cut(s) 142
TaqI TCGA 1 cut(s) 136
TasI AATT 8 cut(s) 7, 14, 77, 394, 444, 546, 584, 636
Tru1I TTAA 4 cut(s) 171, 207, 324, 560
Tru9I TTAA 4 cut(s) 171, 207, 324, 560
TscAI CASTG 2 cut(s) 129, 244
TspDTI ATGAA 4 cut(s) 281, 429, 486, 645
TspRI CASTG 2 cut(s) 129, 244
Vha464I CTTAAG 1 cut(s) 559
XapI RAATTY 1 cut(s) 7
XceI RCATGY 2 cut(s) 122, 426
XmiI GTMKAC 1 cut(s) 319
XmnI GAANNNNTTC 1 cut(s) 550
ZraI GACGTC 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.