Rroxscaffold_4G00326330

tRNA-dihydrouridine20 synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001304
Physical Location & Seq
Forward (+)
58383107 .. 58394068
10962 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00326330.1

Sequence Viewer

Length: 669 bp
ATGTCGAATTCTGAATTAGAGGACAAGATTTTCAACTTGTTCAAGGATTTTATGGTCGGGATTGCCAAGTTTGAGGAATTGGTGCCCATGGGAAGCACATTTCTCAATGGCTTTCAGCATGCACTGGAGTTTCTTCGACGGCCGGCGATAGATAAAACATCTGAGTTGGTTAAAAATATAATCCAATCAAACGAAACAAGGAGGGTTAAATCATACATTGAAGCTGGATGTATCAACACTGATGATGGCAGGCACAATATAACCAAGTTGCAATCATGCCAACTTGGACTTCATGACCATATATGTAAAGGAAAATGTATACTTAACGAACTGGAATGCCTACTAGAAGATGTAAGTGGTGCAATGCAAGATAAATCATTGTTCCAGGATGACAATTTGGGTGAAAGGTTGAATGAACAAGCATGTATCAATGACAAGGAGGAAATTGCCTCGCCTTGTCCCAGAAGAAATGAAGTTACTGATTATGCTGTCCTGATGGCAGTCATTTATCGTATGGTCAAGCAAGAATATATGATGCAGGAAAGAATTGTTTCTGCGTTAAGTCTCAAGTCATCTTCCGGAGAATTAGAGAGCTACTCCCTTATGTGGTCGTTGCGTCCTTACATAGATGAAGAAATTATGGGTCTTGCTTGGAAACTTATACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

222

Amino Acids

25.45

Weight (kDa)

5.06

Isoelectric Point (pI)

61.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7795 PF25071 4 - 124 2.5e-55 Domain of unknown function (DUF7795)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 82
AccI GTMKAC 1 cut(s) 319
AccIII TCCGGA 1 cut(s) 578
AcoI YGGCCR 1 cut(s) 140
AcsI RAATTY 1 cut(s) 7
AfiI CCNNNNNNNGG 1 cut(s) 606
AgsI TTSAA 4 cut(s) 34, 43, 221, 412
AjnI CCWGG 1 cut(s) 384
AjuI GAANNNNNNNTTGG 2 cut(s) 273, 305
AluBI AGCT 2 cut(s) 224, 594
AluI AGCT 2 cut(s) 224, 594
Alw26I GTCTC 1 cut(s) 569
Aor13HI TCCGGA 1 cut(s) 578
AoxI GGCC 1 cut(s) 140
ApoI RAATTY 1 cut(s) 7
Asp700I GAANNNNTTC 1 cut(s) 550
AsuHPI GGTGA 1 cut(s) 413
BaeGI GKGCMC 1 cut(s) 87
BanI GGYRCC 1 cut(s) 82
BccI CCATC 2 cut(s) 239, 490
BceAI ACGGC 1 cut(s) 155
BciT130I CCWGG 1 cut(s) 386
BcoDI GTCTC 1 cut(s) 569
BfaI CTAG 1 cut(s) 344
Bme1390I CCNGG 1 cut(s) 386
BmiI GGNNCC 1 cut(s) 84
BmrFI CCNGG 1 cut(s) 386
BmsI GCATC 1 cut(s) 525
BplI GAGNNNNNCTC 2 cut(s) 581, 613
BpmI CTGGAG 1 cut(s) 146
BpuEI CTTGAG 1 cut(s) 551
BsaJI CCNNGG 1 cut(s) 87
BsaWI WCCGGW 1 cut(s) 578
Bsc4I CCNNNNNNNGG 1 cut(s) 606
Bse118I RCCGGY 1 cut(s) 142
Bse1I ACTGG 2 cut(s) 129, 336
Bse3DI GCAATG 1 cut(s) 369
BseAI TCCGGA 1 cut(s) 578
BseBI CCWGG 1 cut(s) 386
BseDI CCNNGG 1 cut(s) 87
BseGI GGATG 2 cut(s) 233, 394
BseLI CCNNNNNNNGG 1 cut(s) 606
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 1 cut(s) 153
BseNI ACTGG 2 cut(s) 129, 336
BseSI GKGCMC 1 cut(s) 87
BseX3I CGGCCG 1 cut(s) 140
Bsh1285I CGRYCG 1 cut(s) 143
BshFI GGCC 1 cut(s) 142
BshNI GGYRCC 1 cut(s) 82
BsiEI CGRYCG 1 cut(s) 143
BsiSI CCGG 2 cut(s) 143, 579
BslFI GGGAC 1 cut(s) 444
BslI CCNNNNNNNGG 1 cut(s) 606
BsmAI GTCTC 1 cut(s) 569
BsmFI GGGAC 1 cut(s) 444
BsmI GAATGC 1 cut(s) 341
BsnI GGCC 1 cut(s) 142
Bsp1286I GDGCHC 1 cut(s) 87
Bsp13I TCCGGA 1 cut(s) 578
Bsp19I CCATGG 1 cut(s) 87
BspANI GGCC 1 cut(s) 142
BspCNI CTCAG 1 cut(s) 154
BspEI TCCGGA 1 cut(s) 578
BspHI TCATGA 1 cut(s) 292
BspLI GGNNCC 1 cut(s) 84
BspT107I GGYRCC 1 cut(s) 82
BsrDI GCAATG 1 cut(s) 369
BsrFI RCCGGY 1 cut(s) 142
BsrI ACTGG 2 cut(s) 129, 336
BssAI RCCGGY 1 cut(s) 142
BssECI CCNNGG 1 cut(s) 87
BssNAI GTATAC 1 cut(s) 320
BssT1I CCWWGG 1 cut(s) 87
Bst1107I GTATAC 1 cut(s) 320
Bst2UI CCWGG 1 cut(s) 386
BstC8I GCNNGC 3 cut(s) 120, 144, 251
BstDEI CTNAG 1 cut(s) 162
BstDSI CCRYGG 1 cut(s) 87
BstF5I GGATG 2 cut(s) 233, 394
BstMAI GTCTC 1 cut(s) 569
BstMCI CGRYCG 1 cut(s) 143
BstNI CCWGG 1 cut(s) 386
BstNSI RCATGY 2 cut(s) 122, 426
BstSCI CCNGG 1 cut(s) 384
BstSLI GKGCMC 1 cut(s) 87
BstZ17I GTATAC 1 cut(s) 320
BstZI CGGCCG 1 cut(s) 140
BsuRI GGCC 1 cut(s) 142
BtgI CCRYGG 1 cut(s) 87
BtsCI GGATG 2 cut(s) 233, 394
BtsIMutI CAGTG 3 cut(s) 122, 237, 664
Cac8I GCNNGC 3 cut(s) 120, 144, 251
CciI TCATGA 1 cut(s) 292
Cfr10I RCCGGY 1 cut(s) 142
CseI GACGC 1 cut(s) 605
CviAII CATG 5 cut(s) 88, 119, 276, 293, 423
CviJI RGCY 4 cut(s) 111, 142, 224, 594
CviKI_1 RGCY 4 cut(s) 111, 142, 224, 594
DdeI CTNAG 1 cut(s) 162
EaeI YGGCCR 1 cut(s) 140
EagI CGGCCG 1 cut(s) 140
EclXI CGGCCG 1 cut(s) 140
Eco130I CCWWGG 1 cut(s) 87
Eco52I CGGCCG 1 cut(s) 140
EcoRI GAATTC 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 384
EcoT14I CCWWGG 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 5 cut(s) 91, 122, 279, 296, 426
FaqI GGGAC 1 cut(s) 444
FatI CATG 5 cut(s) 87, 118, 275, 292, 422
FblI GTMKAC 1 cut(s) 319
FokI GGATG 2 cut(s) 240, 401
FspBI CTAG 1 cut(s) 344
GsuI CTGGAG 1 cut(s) 146
HaeIII GGCC 1 cut(s) 142
HapII CCGG 2 cut(s) 143, 579
HgaI GACGC 1 cut(s) 605
Hin1II CATG 5 cut(s) 91, 122, 279, 296, 426
HpaII CCGG 2 cut(s) 143, 579
HphI GGTGA 1 cut(s) 413
Hpy166II GTNNAC 1 cut(s) 320
Hpy188I TCNGA 2 cut(s) 13, 163
Hpy188III TCNNGA 4 cut(s) 58, 293, 493, 579
Hpy8I GTNNAC 1 cut(s) 320
Hpy99I CGWCG 1 cut(s) 141
HpyCH4V TGCA 5 cut(s) 122, 271, 362, 367, 538
HpyF3I CTNAG 1 cut(s) 162
Hsp92II CATG 5 cut(s) 91, 122, 279, 296, 426
Kpn2I TCCGGA 1 cut(s) 578
KroI GCCGGC 1 cut(s) 142
KroNI GCCGGC 1 cut(s) 144
LweI GCATC 1 cut(s) 525
MaeI CTAG 1 cut(s) 344
MaeIII GTNAC 1 cut(s) 475
MboII GAAGA 5 cut(s) 125, 359, 477, 567, 644
MhlI GDGCHC 1 cut(s) 87
MluCI AATT 8 cut(s) 7, 14, 77, 394, 444, 546, 584, 636
MnlI CCTC 5 cut(s) 13, 67, 195, 433, 460
MroI TCCGGA 1 cut(s) 578
MroNI GCCGGC 1 cut(s) 142
MroXI GAANNNNTTC 1 cut(s) 550
MseI TTAA 4 cut(s) 171, 207, 324, 560
MspI CCGG 2 cut(s) 143, 579
MspR9I CCNGG 1 cut(s) 386
Mva1269I GAATGC 1 cut(s) 341
MvaI CCWGG 1 cut(s) 386
NaeI GCCGGC 1 cut(s) 144
NcoI CCATGG 1 cut(s) 87
NgoMIV GCCGGC 1 cut(s) 142
NlaIII CATG 5 cut(s) 91, 122, 279, 296, 426
NlaIV GGNNCC 1 cut(s) 84
NspI RCATGY 2 cut(s) 122, 426
PaeI GCATGC 1 cut(s) 122
PagI TCATGA 1 cut(s) 292
PctI GAATGC 1 cut(s) 341
PdiI GCCGGC 1 cut(s) 144
PdmI GAANNNNTTC 1 cut(s) 550
PfoI TCCNGGA 1 cut(s) 384
Psp6I CCWGG 1 cut(s) 384
PspGI CCWGG 1 cut(s) 384
PspN4I GGNNCC 1 cut(s) 84
SaqAI TTAA 4 cut(s) 171, 207, 324, 560
ScrFI CCNGG 1 cut(s) 386
SduI GDGCHC 1 cut(s) 87
SetI ASST 3 cut(s) 226, 410, 596
SfaNI GCATC 1 cut(s) 525
SmlI CTYRAG 1 cut(s) 566
SmoI CTYRAG 1 cut(s) 566
SphI GCATGC 1 cut(s) 122
Sse9I AATT 8 cut(s) 7, 14, 77, 394, 444, 546, 584, 636
SspMI CTAG 1 cut(s) 344
StyD4I CCNGG 1 cut(s) 384
StyI CCWWGG 1 cut(s) 87
TaqI TCGA 2 cut(s) 5, 136
TasI AATT 8 cut(s) 7, 14, 77, 394, 444, 546, 584, 636
Tru1I TTAA 4 cut(s) 171, 207, 324, 560
Tru9I TTAA 4 cut(s) 171, 207, 324, 560
TscAI CASTG 2 cut(s) 129, 244
TspDTI ATGAA 4 cut(s) 281, 429, 486, 645
TspRI CASTG 2 cut(s) 129, 244
XapI RAATTY 1 cut(s) 7
XceI RCATGY 2 cut(s) 122, 426
XmiI GTMKAC 1 cut(s) 319
XmnI GAANNNNTTC 1 cut(s) 550
XspI CTAG 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.