RchiOBHm_Chr5g0054161

tRNA-dihydrouridine20 synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001304
Physical Location & Seq
Reverse (-)
56612345 .. 56616073
3729 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33137

Sequence Viewer

Length: 699 bp
ATGGTGCATGTAGAAGCATTGACTGCTTTGGCATCAGTTGTTGTTTCATTTCCGATGTCGAATTCTGAATTAGAGGACAAGATTTTCAGCTTGTTCAAGGATTTTATGGCCGGGATTGCCAAGTTTGAGGAATTGGTGCCCATGGGAAGCACATTTCTCAATGGCTTTCAGCATGCACTGGATAAAACATCTGACTTGGTTAAAAATATAATCCAATCAAACGAAACAAAGAGGGTTAAATCATACATTGAAGCTGGATGTATCAACACTGATGATGGCAGGCACAATATAACCAAGTTGCAATCATGCCAACTTGGACTTCATGACCATATATGTAAAGGAAAAGGTATACTTAACGAACTGGAATGCCTACTGGAAGATGTAAGTGGTGCAATGCAAGATAAATCCTTGTTCCAGGATGACAATTTGGGTGAAAGATTGAATGAACAAGCATGTATCAATGACAAGGAAGAAATTGCCTCGCCTTGTCCCAGAAGAAATGAAGTTACTGATTATGCTATCCTGATGGCAGTCATTTATCATATGGTCAAGCAAGAATATATGATGCAGGAAAGAATTGTTTCTGCATTAAGTCTCAAGTCATCTTCCGGAGAATTAGAGAGCTACTGCCTTATGTGGTCTTTGCGTCCTTACATAGATGAAGAAATTATGGGTCATGCTTGGAAACTTATACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

232

Amino Acids

26.21

Weight (kDa)

5.02

Isoelectric Point (pI)

55.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7795 PF25071 22 - 134 7.8e-45 Domain of unknown function (DUF7795)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 136
AccI GTMKAC 1 cut(s) 349
AccIII TCCGGA 1 cut(s) 608
AcoI YGGCCR 1 cut(s) 108
AcsI RAATTY 1 cut(s) 61
AgsI TTSAA 3 cut(s) 97, 251, 442
AjnI CCWGG 1 cut(s) 414
AjuI GAANNNNNNNTTGG 2 cut(s) 303, 335
AluBI AGCT 3 cut(s) 90, 254, 624
AluI AGCT 3 cut(s) 90, 254, 624
Alw26I GTCTC 1 cut(s) 599
Aor13HI TCCGGA 1 cut(s) 608
AoxI GGCC 1 cut(s) 108
ApoI RAATTY 1 cut(s) 61
Asp700I GAANNNNTTC 1 cut(s) 580
AsuC2I CCSGG 1 cut(s) 112
AsuHPI GGTGA 1 cut(s) 443
BaeGI GKGCMC 1 cut(s) 141
BanI GGYRCC 1 cut(s) 136
BccI CCATC 2 cut(s) 269, 520
BciT130I CCWGG 1 cut(s) 416
BcnI CCSGG 1 cut(s) 112
BcoDI GTCTC 1 cut(s) 599
Bme1390I CCNGG 2 cut(s) 112, 416
BmiI GGNNCC 1 cut(s) 138
BmrFI CCNGG 2 cut(s) 112, 416
BmsI GCATC 2 cut(s) 41, 555
BpuEI CTTGAG 1 cut(s) 581
BpuMI CCSGG 1 cut(s) 112
BsaJI CCNNGG 1 cut(s) 141
BsaWI WCCGGW 1 cut(s) 608
Bse1I ACTGG 3 cut(s) 183, 366, 378
Bse3DI GCAATG 1 cut(s) 399
BseAI TCCGGA 1 cut(s) 608
BseBI CCWGG 1 cut(s) 416
BseDI CCNNGG 1 cut(s) 141
BseGI GGATG 2 cut(s) 263, 424
BseMI GCAATG 1 cut(s) 399
BseNI ACTGG 3 cut(s) 183, 366, 378
BseSI GKGCMC 1 cut(s) 141
BshFI GGCC 1 cut(s) 110
BshNI GGYRCC 1 cut(s) 136
BsiSI CCGG 2 cut(s) 111, 609
BslFI GGGAC 1 cut(s) 474
BsmAI GTCTC 1 cut(s) 599
BsmFI GGGAC 1 cut(s) 474
BsmI GAATGC 1 cut(s) 371
BsnI GGCC 1 cut(s) 110
Bsp1286I GDGCHC 1 cut(s) 141
Bsp13I TCCGGA 1 cut(s) 608
Bsp19I CCATGG 1 cut(s) 141
BspANI GGCC 1 cut(s) 110
BspEI TCCGGA 1 cut(s) 608
BspHI TCATGA 1 cut(s) 322
BspLI GGNNCC 1 cut(s) 138
BspT107I GGYRCC 1 cut(s) 136
BsrDI GCAATG 1 cut(s) 399
BsrI ACTGG 3 cut(s) 183, 366, 378
BssECI CCNNGG 1 cut(s) 141
BssNAI GTATAC 1 cut(s) 350
BssT1I CCWWGG 1 cut(s) 141
Bst1107I GTATAC 1 cut(s) 350
Bst2UI CCWGG 1 cut(s) 416
BstAPI GCANNNNNTGC 1 cut(s) 23
BstC8I GCNNGC 2 cut(s) 174, 281
BstDSI CCRYGG 1 cut(s) 141
BstF5I GGATG 2 cut(s) 263, 424
BstMAI GTCTC 1 cut(s) 599
BstMWI GCNNNNNNNGC 2 cut(s) 23, 116
BstNI CCWGG 1 cut(s) 416
BstNSI RCATGY 3 cut(s) 11, 176, 456
BstSCI CCNGG 2 cut(s) 110, 414
BstSLI GKGCMC 1 cut(s) 141
BstZ17I GTATAC 1 cut(s) 350
BsuRI GGCC 1 cut(s) 110
BtgI CCRYGG 1 cut(s) 141
BtsCI GGATG 2 cut(s) 263, 424
BtsIMutI CAGTG 3 cut(s) 176, 267, 694
Cac8I GCNNGC 2 cut(s) 174, 281
CciI TCATGA 1 cut(s) 322
CseI GACGC 1 cut(s) 635
CviAII CATG 7 cut(s) 8, 142, 173, 306, 323, 453, 677
CviJI RGCY 5 cut(s) 90, 110, 165, 254, 624
CviKI_1 RGCY 5 cut(s) 90, 110, 165, 254, 624
EaeI YGGCCR 1 cut(s) 108
Eco130I CCWWGG 1 cut(s) 141
EcoRI GAATTC 1 cut(s) 61
EcoRII CCWGG 1 cut(s) 414
EcoT14I CCWWGG 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 141
FaeI CATG 7 cut(s) 11, 145, 176, 309, 326, 456, 680
FalI AAGNNNNNCTT 2 cut(s) 336, 368
FaqI GGGAC 1 cut(s) 474
FatI CATG 7 cut(s) 7, 141, 172, 305, 322, 452, 676
FauNDI CATATG 1 cut(s) 543
FblI GTMKAC 1 cut(s) 349
FokI GGATG 2 cut(s) 270, 431
HaeIII GGCC 1 cut(s) 110
HapII CCGG 2 cut(s) 111, 609
HgaI GACGC 1 cut(s) 635
Hin1II CATG 7 cut(s) 11, 145, 176, 309, 326, 456, 680
HpaII CCGG 2 cut(s) 111, 609
HphI GGTGA 1 cut(s) 443
Hpy166II GTNNAC 1 cut(s) 350
Hpy188I TCNGA 3 cut(s) 54, 67, 193
Hpy188III TCNNGA 3 cut(s) 323, 523, 609
Hpy8I GTNNAC 1 cut(s) 350
HpyCH4V TGCA 7 cut(s) 7, 176, 301, 392, 397, 568, 587
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 116
Hsp92II CATG 7 cut(s) 11, 145, 176, 309, 326, 456, 680
Kpn2I TCCGGA 1 cut(s) 608
LweI GCATC 2 cut(s) 41, 555
MaeIII GTNAC 1 cut(s) 505
MboII GAAGA 5 cut(s) 389, 482, 507, 597, 674
MhlI GDGCHC 1 cut(s) 141
MluCI AATT 8 cut(s) 61, 68, 131, 424, 474, 576, 614, 666
MnlI CCTC 4 cut(s) 67, 121, 225, 490
MroI TCCGGA 1 cut(s) 608
MroXI GAANNNNTTC 1 cut(s) 580
MseI TTAA 4 cut(s) 201, 237, 354, 590
MspI CCGG 2 cut(s) 111, 609
MspR9I CCNGG 2 cut(s) 112, 416
Mva1269I GAATGC 1 cut(s) 371
MvaI CCWGG 1 cut(s) 416
MwoI GCNNNNNNNGC 2 cut(s) 23, 116
NciI CCSGG 1 cut(s) 112
NcoI CCATGG 1 cut(s) 141
NdeI CATATG 1 cut(s) 543
NlaIII CATG 7 cut(s) 11, 145, 176, 309, 326, 456, 680
NlaIV GGNNCC 1 cut(s) 138
NspI RCATGY 3 cut(s) 11, 176, 456
PaeI GCATGC 1 cut(s) 176
PagI TCATGA 1 cut(s) 322
PctI GAATGC 1 cut(s) 371
PdmI GAANNNNTTC 1 cut(s) 580
PfoI TCCNGGA 1 cut(s) 414
Psp6I CCWGG 1 cut(s) 414
PspGI CCWGG 1 cut(s) 414
PspN4I GGNNCC 1 cut(s) 138
SaqAI TTAA 4 cut(s) 201, 237, 354, 590
ScrFI CCNGG 2 cut(s) 112, 416
SduI GDGCHC 1 cut(s) 141
SetI ASST 4 cut(s) 92, 256, 349, 626
SfaNI GCATC 2 cut(s) 41, 555
SmlI CTYRAG 1 cut(s) 596
SmoI CTYRAG 1 cut(s) 596
SphI GCATGC 1 cut(s) 176
Sse9I AATT 8 cut(s) 61, 68, 131, 424, 474, 576, 614, 666
StyD4I CCNGG 2 cut(s) 110, 414
StyI CCWWGG 1 cut(s) 141
TaqI TCGA 1 cut(s) 59
TasI AATT 8 cut(s) 61, 68, 131, 424, 474, 576, 614, 666
Tru1I TTAA 4 cut(s) 201, 237, 354, 590
Tru9I TTAA 4 cut(s) 201, 237, 354, 590
TscAI CASTG 2 cut(s) 183, 274
TspDTI ATGAA 5 cut(s) 36, 311, 459, 516, 675
TspRI CASTG 2 cut(s) 183, 274
XapI RAATTY 1 cut(s) 61
XceI RCATGY 3 cut(s) 11, 176, 456
XmiI GTMKAC 1 cut(s) 349
XmnI GAANNNNTTC 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.