Rh2DG220000

tRNA-dihydrouridine20 synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001304
Physical Location & Seq
Forward (+)
21134963 .. 21137168
2206 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG220000.1

Sequence Viewer

Length: 633 bp
ATGTTTTACCTTCATTGTGGTAGGATTGCCAAGTTTGAGGAATTGGTGCCCATGGGAAGCACATTTCTCAATGGCTTTCAGCATGCACTGGAGTTTCTTCGACGTCCGGCGATAGATAAAACATCTGAGTTGGTTAAAAATATAATCCAATCTAACGAAACAAAGAGGGTTAAATCATACATTGAAGCTGGATGTATCAACACTGATGATGGCAGGCACAATATAACCAAGTTGCAATCATGCCAACTTGGACTTCATGACCATATATGTAAAGGAAAAGGTATACTTAACGAACTGGAATGCCTACTGGAAGATGTAAGTGGTGCAATGCAAGATAAATCCTTGTTCCAGGATGACAATTTGGGTGAAAGGTTGAATGAACAAGCATGTATCAATGACAAGGAGGAAATTGCGTCTCCTTGTCCCAGAAGAAATGAAGTTACTGATTATGCTGTCCTGATGGCAGTCATTTATCGTATGGTCAAGCAAGAATATATGATGCAGGAAAGAATTGTTTTTGCCTTAAGTCTCAAGTCATCTTCCGGAGAATTAGAGAGCTACTGCCTTATGTGGTCTTTGCGTCCTTACATAGATGAAGAAATTATGGGTCATGCTTGGAAACTTATACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

210

Amino Acids

24.14

Weight (kDa)

5.53

Isoelectric Point (pI)

63.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7795 PF25071 7 - 112 2.4e-48 Domain of unknown function (DUF7795)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 106
AccB1I GGYRCC 1 cut(s) 46
AccI GTMKAC 1 cut(s) 283
AccIII TCCGGA 1 cut(s) 542
AcyI GRCGYC 1 cut(s) 103
AflII CTTAAG 1 cut(s) 523
AgsI TTSAA 2 cut(s) 185, 376
AjnI CCWGG 1 cut(s) 348
AjuI GAANNNNNNNTTGG 2 cut(s) 237, 269
AluBI AGCT 2 cut(s) 188, 558
AluI AGCT 2 cut(s) 188, 558
Alw26I GTCTC 2 cut(s) 420, 533
Aor13HI TCCGGA 1 cut(s) 542
AsuHPI GGTGA 1 cut(s) 377
BaeGI GKGCMC 1 cut(s) 51
BanI GGYRCC 1 cut(s) 46
BccI CCATC 2 cut(s) 203, 454
BciT130I CCWGG 1 cut(s) 350
BcoDI GTCTC 2 cut(s) 420, 533
BfrI CTTAAG 1 cut(s) 523
Bme1390I CCNGG 1 cut(s) 350
BmiI GGNNCC 1 cut(s) 48
BmrFI CCNGG 1 cut(s) 350
BmsI GCATC 1 cut(s) 489
BpmI CTGGAG 1 cut(s) 110
BpuEI CTTGAG 1 cut(s) 515
BsaHI GRCGYC 1 cut(s) 103
BsaJI CCNNGG 1 cut(s) 51
BsaWI WCCGGW 1 cut(s) 542
Bse1I ACTGG 3 cut(s) 93, 300, 312
Bse3DI GCAATG 1 cut(s) 333
BseAI TCCGGA 1 cut(s) 542
BseBI CCWGG 1 cut(s) 350
BseDI CCNNGG 1 cut(s) 51
BseGI GGATG 2 cut(s) 197, 358
BseMI GCAATG 1 cut(s) 333
BseMII CTCAG 1 cut(s) 117
BseNI ACTGG 3 cut(s) 93, 300, 312
BseSI GKGCMC 1 cut(s) 51
BshNI GGYRCC 1 cut(s) 46
BsiSI CCGG 2 cut(s) 107, 543
BslFI GGGAC 1 cut(s) 408
BsmAI GTCTC 2 cut(s) 420, 533
BsmBI CGTCTC 1 cut(s) 420
BsmFI GGGAC 1 cut(s) 408
BsmI GAATGC 1 cut(s) 305
Bsp1286I GDGCHC 1 cut(s) 51
Bsp13I TCCGGA 1 cut(s) 542
Bsp19I CCATGG 1 cut(s) 51
BspCNI CTCAG 1 cut(s) 118
BspEI TCCGGA 1 cut(s) 542
BspHI TCATGA 1 cut(s) 256
BspLI GGNNCC 1 cut(s) 48
BspT107I GGYRCC 1 cut(s) 46
BspTI CTTAAG 1 cut(s) 523
BsrDI GCAATG 1 cut(s) 333
BsrI ACTGG 3 cut(s) 93, 300, 312
BssECI CCNNGG 1 cut(s) 51
BssNAI GTATAC 1 cut(s) 284
BssNI GRCGYC 1 cut(s) 103
BssT1I CCWWGG 1 cut(s) 51
Bst1107I GTATAC 1 cut(s) 284
Bst2UI CCWGG 1 cut(s) 350
BstACI GRCGYC 1 cut(s) 103
BstAFI CTTAAG 1 cut(s) 523
BstC8I GCNNGC 2 cut(s) 84, 215
BstDEI CTNAG 1 cut(s) 126
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 2 cut(s) 197, 358
BstMAI GTCTC 2 cut(s) 420, 533
BstNI CCWGG 1 cut(s) 350
BstNSI RCATGY 2 cut(s) 86, 390
BstSCI CCNGG 1 cut(s) 348
BstSLI GKGCMC 1 cut(s) 51
BstZ17I GTATAC 1 cut(s) 284
BtgI CCRYGG 1 cut(s) 51
BtsCI GGATG 2 cut(s) 197, 358
BtsIMutI CAGTG 3 cut(s) 86, 201, 628
Cac8I GCNNGC 2 cut(s) 84, 215
CciI TCATGA 1 cut(s) 256
CseI GACGC 2 cut(s) 402, 569
CviAII CATG 6 cut(s) 52, 83, 240, 257, 387, 611
CviJI RGCY 3 cut(s) 75, 188, 558
CviKI_1 RGCY 3 cut(s) 75, 188, 558
DdeI CTNAG 1 cut(s) 126
Eco130I CCWWGG 1 cut(s) 51
EcoRII CCWGG 1 cut(s) 348
EcoT14I CCWWGG 1 cut(s) 51
ErhI CCWWGG 1 cut(s) 51
Esp3I CGTCTC 1 cut(s) 420
FaeI CATG 6 cut(s) 55, 86, 243, 260, 390, 614
FalI AAGNNNNNCTT 2 cut(s) 270, 302
FaqI GGGAC 1 cut(s) 408
FatI CATG 6 cut(s) 51, 82, 239, 256, 386, 610
FblI GTMKAC 1 cut(s) 283
FokI GGATG 2 cut(s) 204, 365
GsuI CTGGAG 1 cut(s) 110
HapII CCGG 2 cut(s) 107, 543
HgaI GACGC 2 cut(s) 402, 569
Hin1I GRCGYC 1 cut(s) 103
Hin1II CATG 6 cut(s) 55, 86, 243, 260, 390, 614
HpaII CCGG 2 cut(s) 107, 543
HphI GGTGA 1 cut(s) 377
Hpy166II GTNNAC 1 cut(s) 284
Hpy188I TCNGA 1 cut(s) 127
Hpy188III TCNNGA 3 cut(s) 257, 457, 543
Hpy8I GTNNAC 1 cut(s) 284
Hpy99I CGWCG 1 cut(s) 105
HpyAV CCTTC 1 cut(s) 20
HpyCH4IV ACGT 1 cut(s) 103
HpyCH4V TGCA 5 cut(s) 86, 235, 326, 331, 502
HpyF3I CTNAG 1 cut(s) 126
HpySE526I ACGT 1 cut(s) 103
Hsp92I GRCGYC 1 cut(s) 103
Hsp92II CATG 6 cut(s) 55, 86, 243, 260, 390, 614
Kpn2I TCCGGA 1 cut(s) 542
LweI GCATC 1 cut(s) 489
MaeII ACGT 1 cut(s) 103
MaeIII GTNAC 1 cut(s) 439
MboII GAAGA 5 cut(s) 89, 323, 441, 531, 608
MhlI GDGCHC 1 cut(s) 51
MluCI AATT 6 cut(s) 41, 358, 408, 510, 548, 600
MnlI CCTC 3 cut(s) 31, 159, 397
MroI TCCGGA 1 cut(s) 542
MseI TTAA 4 cut(s) 135, 171, 288, 524
MspCI CTTAAG 1 cut(s) 523
MspI CCGG 2 cut(s) 107, 543
MspR9I CCNGG 1 cut(s) 350
Mva1269I GAATGC 1 cut(s) 305
MvaI CCWGG 1 cut(s) 350
NcoI CCATGG 1 cut(s) 51
NlaIII CATG 6 cut(s) 55, 86, 243, 260, 390, 614
NlaIV GGNNCC 1 cut(s) 48
NspI RCATGY 2 cut(s) 86, 390
PaeI GCATGC 1 cut(s) 86
PagI TCATGA 1 cut(s) 256
PctI GAATGC 1 cut(s) 305
PfoI TCCNGGA 1 cut(s) 348
Psp6I CCWGG 1 cut(s) 348
PspGI CCWGG 1 cut(s) 348
PspN4I GGNNCC 1 cut(s) 48
SaqAI TTAA 4 cut(s) 135, 171, 288, 524
ScrFI CCNGG 1 cut(s) 350
SduI GDGCHC 1 cut(s) 51
SetI ASST 6 cut(s) 12, 106, 190, 283, 374, 560
SfaNI GCATC 1 cut(s) 489
SmlI CTYRAG 2 cut(s) 523, 530
SmoI CTYRAG 2 cut(s) 523, 530
SphI GCATGC 1 cut(s) 86
Sse9I AATT 6 cut(s) 41, 358, 408, 510, 548, 600
StyD4I CCNGG 1 cut(s) 348
StyI CCWWGG 1 cut(s) 51
TaiI ACGT 1 cut(s) 106
TaqI TCGA 1 cut(s) 100
TasI AATT 6 cut(s) 41, 358, 408, 510, 548, 600
Tru1I TTAA 4 cut(s) 135, 171, 288, 524
Tru9I TTAA 4 cut(s) 135, 171, 288, 524
TscAI CASTG 2 cut(s) 93, 208
TspDTI ATGAA 4 cut(s) 245, 393, 450, 609
TspRI CASTG 2 cut(s) 93, 208
Vha464I CTTAAG 1 cut(s) 523
XceI RCATGY 2 cut(s) 86, 390
XmiI GTMKAC 1 cut(s) 283
ZraI GACGTC 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.