RLG00000030260

tRNA-dihydrouridine20 synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001304
Physical Location & Seq
Forward (+)
58515717 .. 58544479
28763 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030260

Sequence Viewer

Length: 672 bp
ATGACAAGATTTTCAGCTTGTTCAAGGATTTTATGGCCGGTTATACGCTTAACTCTGCTAATTGCCAAGTTTGAGGAATTGGTGCCCATGGGAAGCACATTTCTCAATGGCTTTCAGCATGCACTGGAGTTTCTTCGACGGCCGGCGATAGATAAAACATCTGAGTTGGTTAAAAATATAATCCAATCAAACGAAACAAAGAGGGTTAAATCATACATTGAAGCTGGATGTATCAACACTGATGATTGCAGGCACAATATAACCAAGTTAGTGCAATCATGCCAACTTGGACTTCATGACCATATATGTAAAGGAAAATGTATACTTAACGAACTGGAATGCCTACTAGAAGATGTAAGTGGTGCAATGCAAGATAAATCATTGTTCCAGGATGACAATTTGGGTGAAAGGTTGAATGAACAAGCATGTATCAATGACAAGGAGGAAATTGCCTCGCCTTGTCCTAGAAGAAATGAAGTTACTGATTATGCTGTCCTGATGGCAGTCATTTATCGTATGGTCAAGCATGAATATATGATGCAGGAAAGAATTGTTTCTGCGTTAAGTCTCAAGTCATCTTCCGGAGAATTAGAGAGCTACTCCCTGATGTGGTCTTTGCGTCCTTACATAGATGAAGAAATTATGGGTCTTGCTTGGAAACTTATACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

224

Amino Acids

25.59

Weight (kDa)

5.73

Isoelectric Point (pI)

60.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7795 PF25071 21 - 125 1.1e-45 Domain of unknown function (DUF7795)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 82
AccI GTMKAC 1 cut(s) 322
AccIII TCCGGA 1 cut(s) 581
AcoI YGGCCR 2 cut(s) 35, 140
AfiI CCNNNNNNNGG 1 cut(s) 609
AgsI TTSAA 3 cut(s) 24, 221, 415
AjnI CCWGG 1 cut(s) 387
AjuI GAANNNNNNNTTGG 2 cut(s) 276, 308
AluBI AGCT 3 cut(s) 17, 224, 597
AluI AGCT 3 cut(s) 17, 224, 597
Alw26I GTCTC 1 cut(s) 572
Aor13HI TCCGGA 1 cut(s) 581
AoxI GGCC 2 cut(s) 35, 140
Asp700I GAANNNNTTC 1 cut(s) 553
AsuHPI GGTGA 1 cut(s) 416
BaeGI GKGCMC 1 cut(s) 87
BanI GGYRCC 1 cut(s) 82
BccI CCATC 1 cut(s) 493
BceAI ACGGC 1 cut(s) 155
BciT130I CCWGG 1 cut(s) 389
BcoDI GTCTC 1 cut(s) 572
BfaI CTAG 2 cut(s) 347, 465
Bme1390I CCNGG 1 cut(s) 389
BmiI GGNNCC 1 cut(s) 84
BmrFI CCNGG 1 cut(s) 389
BmsI GCATC 1 cut(s) 528
BplI GAGNNNNNCTC 2 cut(s) 584, 616
BpmI CTGGAG 1 cut(s) 146
BpuEI CTTGAG 1 cut(s) 554
BsaJI CCNNGG 1 cut(s) 87
BsaWI WCCGGW 1 cut(s) 581
Bsc4I CCNNNNNNNGG 1 cut(s) 609
Bse118I RCCGGY 2 cut(s) 37, 142
Bse1I ACTGG 2 cut(s) 129, 339
Bse3DI GCAATG 1 cut(s) 372
BseAI TCCGGA 1 cut(s) 581
BseBI CCWGG 1 cut(s) 389
BseDI CCNNGG 1 cut(s) 87
BseGI GGATG 2 cut(s) 233, 397
BseLI CCNNNNNNNGG 1 cut(s) 609
BseMI GCAATG 1 cut(s) 372
BseMII CTCAG 1 cut(s) 153
BseNI ACTGG 2 cut(s) 129, 339
BseSI GKGCMC 1 cut(s) 87
BseX3I CGGCCG 1 cut(s) 140
Bsh1285I CGRYCG 1 cut(s) 143
BshFI GGCC 2 cut(s) 37, 142
BshNI GGYRCC 1 cut(s) 82
BsiEI CGRYCG 1 cut(s) 143
BsiSI CCGG 3 cut(s) 38, 143, 582
BslI CCNNNNNNNGG 1 cut(s) 609
BsmAI GTCTC 1 cut(s) 572
BsmI GAATGC 1 cut(s) 344
BsnI GGCC 2 cut(s) 37, 142
Bsp1286I GDGCHC 1 cut(s) 87
Bsp13I TCCGGA 1 cut(s) 581
Bsp19I CCATGG 1 cut(s) 87
BspANI GGCC 2 cut(s) 37, 142
BspCNI CTCAG 1 cut(s) 154
BspEI TCCGGA 1 cut(s) 581
BspHI TCATGA 1 cut(s) 295
BspLI GGNNCC 1 cut(s) 84
BspT107I GGYRCC 1 cut(s) 82
BsrDI GCAATG 1 cut(s) 372
BsrFI RCCGGY 2 cut(s) 37, 142
BsrI ACTGG 2 cut(s) 129, 339
BssAI RCCGGY 2 cut(s) 37, 142
BssECI CCNNGG 1 cut(s) 87
BssNAI GTATAC 1 cut(s) 323
BssT1I CCWWGG 1 cut(s) 87
Bst1107I GTATAC 1 cut(s) 323
Bst2UI CCWGG 1 cut(s) 389
BstC8I GCNNGC 3 cut(s) 120, 144, 251
BstDEI CTNAG 1 cut(s) 162
BstDSI CCRYGG 1 cut(s) 87
BstF5I GGATG 2 cut(s) 233, 397
BstMAI GTCTC 1 cut(s) 572
BstMCI CGRYCG 1 cut(s) 143
BstNI CCWGG 1 cut(s) 389
BstNSI RCATGY 2 cut(s) 122, 429
BstSCI CCNGG 1 cut(s) 387
BstSLI GKGCMC 1 cut(s) 87
BstZ17I GTATAC 1 cut(s) 323
BstZI CGGCCG 1 cut(s) 140
BsuRI GGCC 2 cut(s) 37, 142
BtgI CCRYGG 1 cut(s) 87
BtsCI GGATG 2 cut(s) 233, 397
BtsIMutI CAGTG 3 cut(s) 122, 237, 667
Cac8I GCNNGC 3 cut(s) 120, 144, 251
CciI TCATGA 1 cut(s) 295
Cfr10I RCCGGY 2 cut(s) 37, 142
CseI GACGC 1 cut(s) 608
CviAII CATG 6 cut(s) 88, 119, 279, 296, 426, 527
CviJI RGCY 6 cut(s) 17, 37, 111, 142, 224, 597
CviKI_1 RGCY 6 cut(s) 17, 37, 111, 142, 224, 597
DdeI CTNAG 1 cut(s) 162
EaeI YGGCCR 2 cut(s) 35, 140
EagI CGGCCG 1 cut(s) 140
EclXI CGGCCG 1 cut(s) 140
Eco130I CCWWGG 1 cut(s) 87
Eco52I CGGCCG 1 cut(s) 140
EcoRII CCWGG 1 cut(s) 387
EcoT14I CCWWGG 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 6 cut(s) 91, 122, 282, 299, 429, 530
FatI CATG 6 cut(s) 87, 118, 278, 295, 425, 526
FblI GTMKAC 1 cut(s) 322
FokI GGATG 2 cut(s) 240, 404
FspBI CTAG 2 cut(s) 347, 465
GsuI CTGGAG 1 cut(s) 146
HaeIII GGCC 2 cut(s) 37, 142
HapII CCGG 3 cut(s) 38, 143, 582
HgaI GACGC 1 cut(s) 608
Hin1II CATG 6 cut(s) 91, 122, 282, 299, 429, 530
HpaII CCGG 3 cut(s) 38, 143, 582
HphI GGTGA 1 cut(s) 416
Hpy166II GTNNAC 1 cut(s) 323
Hpy188I TCNGA 1 cut(s) 163
Hpy188III TCNNGA 3 cut(s) 296, 496, 582
Hpy8I GTNNAC 1 cut(s) 323
Hpy99I CGWCG 1 cut(s) 141
HpyCH4V TGCA 6 cut(s) 122, 249, 274, 365, 370, 541
HpyF3I CTNAG 1 cut(s) 162
Hsp92II CATG 6 cut(s) 91, 122, 282, 299, 429, 530
Kpn2I TCCGGA 1 cut(s) 581
KroI GCCGGC 1 cut(s) 142
KroNI GCCGGC 1 cut(s) 144
LweI GCATC 1 cut(s) 528
MaeI CTAG 2 cut(s) 347, 465
MaeIII GTNAC 1 cut(s) 478
MboII GAAGA 5 cut(s) 125, 362, 480, 570, 647
MhlI GDGCHC 1 cut(s) 87
MluCI AATT 7 cut(s) 60, 77, 397, 447, 549, 587, 639
MnlI CCTC 4 cut(s) 67, 195, 436, 463
MroI TCCGGA 1 cut(s) 581
MroNI GCCGGC 1 cut(s) 142
MroXI GAANNNNTTC 1 cut(s) 553
MseI TTAA 5 cut(s) 50, 171, 207, 327, 563
MspI CCGG 3 cut(s) 38, 143, 582
MspR9I CCNGG 1 cut(s) 389
Mva1269I GAATGC 1 cut(s) 344
MvaI CCWGG 1 cut(s) 389
NaeI GCCGGC 1 cut(s) 144
NcoI CCATGG 1 cut(s) 87
NgoMIV GCCGGC 1 cut(s) 142
NlaIII CATG 6 cut(s) 91, 122, 282, 299, 429, 530
NlaIV GGNNCC 1 cut(s) 84
NspI RCATGY 2 cut(s) 122, 429
PaeI GCATGC 1 cut(s) 122
PagI TCATGA 1 cut(s) 295
PctI GAATGC 1 cut(s) 344
PdiI GCCGGC 1 cut(s) 144
PdmI GAANNNNTTC 1 cut(s) 553
PfoI TCCNGGA 1 cut(s) 387
Psp6I CCWGG 1 cut(s) 387
PspGI CCWGG 1 cut(s) 387
PspN4I GGNNCC 1 cut(s) 84
SaqAI TTAA 5 cut(s) 50, 171, 207, 327, 563
ScrFI CCNGG 1 cut(s) 389
SduI GDGCHC 1 cut(s) 87
SetI ASST 4 cut(s) 19, 226, 413, 599
SfaNI GCATC 1 cut(s) 528
SmlI CTYRAG 1 cut(s) 569
SmoI CTYRAG 1 cut(s) 569
SphI GCATGC 1 cut(s) 122
Sse9I AATT 7 cut(s) 60, 77, 397, 447, 549, 587, 639
SspMI CTAG 2 cut(s) 347, 465
StyD4I CCNGG 1 cut(s) 387
StyI CCWWGG 1 cut(s) 87
TaqI TCGA 1 cut(s) 136
TasI AATT 7 cut(s) 60, 77, 397, 447, 549, 587, 639
Tru1I TTAA 5 cut(s) 50, 171, 207, 327, 563
Tru9I TTAA 5 cut(s) 50, 171, 207, 327, 563
TscAI CASTG 2 cut(s) 129, 244
TspDTI ATGAA 5 cut(s) 284, 432, 489, 543, 648
TspRI CASTG 2 cut(s) 129, 244
XceI RCATGY 2 cut(s) 122, 429
XmiI GTMKAC 1 cut(s) 322
XmnI GAANNNNTTC 1 cut(s) 553
XspI CTAG 2 cut(s) 347, 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.