FvH4_2g30460

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001549
Physical Location & Seq
Forward (+)
23599548 .. 23600979
1432 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g30460.t1

Sequence Viewer

Length: 519 bp
ATGGATATGATCCTGGCTACGGTTACAGTTACGAGAGATAATTTATGTAATTGTGGCTTTGATGGTGGGAATGGACGCCTTGATAAGCTAAACATGGGCGCATTGTTTTTGAAACTGGTGTTTTTGTTCATGTCCGTGACCATATTAGAAGCCAAAACACATGTTCGCATCATTAACGATTTCCCGGACCACTCGGTACTATCCGTCCACTGTCGATCTAAGAACAATGATCTTGGCTGGCATTACCTCTCCTACAATAGCTCCTATGAATTTCGTTTTAGGCCCAACTTGTGGGGGACGACTCTATTTGCCTGTGAGATGAAGTGGCCTCCTTCAAATAGTCACGGTGTCGTAATCTATCGTTTCAAAAGAGACTATGACTGTCATTTTACAGGTGGCACATGTACGTGGTACATAAAACCAACTGGTCCGTGTATGTTTATGAAAGATTACCCCATGATGTGTTTGGGTTGGGATCAGCCGCCCAATTACCGTATGGAAATGCAAAATGGAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

173

Amino Acids

19.98

Weight (kDa)

8.11

Isoelectric Point (pI)

29.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 54 - 155 4.6e-27 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 291
AciI CCGC 1 cut(s) 482
AclWI GGATC 2 cut(s) 4, 483
AcsI RAATTY 1 cut(s) 269
AcyI GRCGYC 1 cut(s) 76
AfaI GTAC 3 cut(s) 198, 406, 413
AfiI CCNNNNNNNGG 2 cut(s) 19, 291
AflIII ACRYGT 2 cut(s) 160, 401
AgsI TTSAA 3 cut(s) 112, 336, 367
AjnI CCWGG 1 cut(s) 12
AluBI AGCT 2 cut(s) 88, 261
AluI AGCT 2 cut(s) 88, 261
Alw26I GTCTC 1 cut(s) 366
AlwI GGATC 2 cut(s) 4, 483
AoxI GGCC 2 cut(s) 281, 326
ApoI RAATTY 1 cut(s) 269
AspLEI GCGC 1 cut(s) 101
AspS9I GGNCC 3 cut(s) 187, 282, 428
AsuC2I CCSGG 1 cut(s) 185
AvaII GGWCC 2 cut(s) 187, 428
BccI CCATC 1 cut(s) 56
BciT130I CCWGG 1 cut(s) 14
BcnI CCSGG 1 cut(s) 185
BcoDI GTCTC 1 cut(s) 366
BisI GCNGC 1 cut(s) 482
BlsI GCNGC 1 cut(s) 483
Bme1390I CCNGG 2 cut(s) 14, 185
Bme18I GGWCC 2 cut(s) 187, 428
BmgT120I GGNCC 3 cut(s) 187, 282, 428
BmrFI CCNGG 2 cut(s) 14, 185
BmsI GCATC 1 cut(s) 177
BpuMI CCSGG 1 cut(s) 185
BsaAI YACGTR 1 cut(s) 408
BsaHI GRCGYC 1 cut(s) 76
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bsc4I CCNNNNNNNGG 2 cut(s) 19, 291
Bse1I ACTGG 2 cut(s) 120, 430
BseBI CCWGG 1 cut(s) 14
BseLI CCNNNNNNNGG 2 cut(s) 19, 291
BseNI ACTGG 2 cut(s) 120, 430
BshFI GGCC 2 cut(s) 283, 328
BsiSI CCGG 1 cut(s) 185
BslFI GGGAC 1 cut(s) 310
BslI CCNNNNNNNGG 2 cut(s) 19, 291
BsmAI GTCTC 1 cut(s) 366
BsmFI GGGAC 1 cut(s) 310
BsnI GGCC 2 cut(s) 283, 328
Bsp143I GATC 4 cut(s) 9, 215, 229, 475
BspACI CCGC 1 cut(s) 482
BspANI GGCC 2 cut(s) 283, 328
BspPI GGATC 2 cut(s) 4, 483
BsrI ACTGG 2 cut(s) 120, 430
BssMI GATC 4 cut(s) 9, 215, 229, 475
BssNI GRCGYC 1 cut(s) 76
Bst2UI CCWGG 1 cut(s) 14
Bst4CI ACNGT 6 cut(s) 22, 28, 212, 347, 383, 494
BstACI GRCGYC 1 cut(s) 76
BstBAI YACGTR 1 cut(s) 408
BstC8I GCNNGC 1 cut(s) 239
BstDEI CTNAG 1 cut(s) 219
BstHHI GCGC 1 cut(s) 101
BstKTI GATC 4 cut(s) 12, 218, 232, 478
BstMAI GTCTC 1 cut(s) 366
BstMBI GATC 4 cut(s) 9, 215, 229, 475
BstNI CCWGG 1 cut(s) 14
BstNSI RCATGY 2 cut(s) 164, 405
BstSCI CCNGG 2 cut(s) 12, 183
BsuRI GGCC 2 cut(s) 283, 328
BtsIMutI CAGTG 1 cut(s) 208
Cac8I GCNNGC 1 cut(s) 239
CfoI GCGC 1 cut(s) 101
Cfr13I GGNCC 3 cut(s) 187, 282, 428
CseI GACGC 1 cut(s) 84
Csp6I GTAC 3 cut(s) 197, 405, 412
CviAII CATG 5 cut(s) 94, 130, 161, 402, 457
CviJI RGCY 9 cut(s) 17, 57, 88, 152, 237, 261, 283, 328, 481
CviKI_1 RGCY 9 cut(s) 17, 57, 88, 152, 237, 261, 283, 328, 481
CviQI GTAC 3 cut(s) 197, 405, 412
DdeI CTNAG 1 cut(s) 219
DpnI GATC 4 cut(s) 11, 217, 231, 477
DpnII GATC 4 cut(s) 9, 215, 229, 475
Eco47I GGWCC 2 cut(s) 187, 428
EcoRII CCWGG 1 cut(s) 12
FaeI CATG 5 cut(s) 97, 133, 164, 405, 460
FaqI GGGAC 1 cut(s) 310
FatI CATG 5 cut(s) 93, 129, 160, 401, 456
Fnu4HI GCNGC 1 cut(s) 482
Fsp4HI GCNGC 1 cut(s) 482
GlaI GCGC 1 cut(s) 100
GluI GCNGC 1 cut(s) 482
HaeIII GGCC 2 cut(s) 283, 328
HapII CCGG 1 cut(s) 185
HgaI GACGC 1 cut(s) 84
HhaI GCGC 1 cut(s) 101
Hin1I GRCGYC 1 cut(s) 76
Hin1II CATG 5 cut(s) 97, 133, 164, 405, 460
Hin6I GCGC 1 cut(s) 99
HinP1I GCGC 1 cut(s) 99
HinfI GANTC 1 cut(s) 301
HpaII CCGG 1 cut(s) 185
Hpy166II GTNNAC 1 cut(s) 208
Hpy8I GTNNAC 1 cut(s) 208
HpyAV CCTTC 1 cut(s) 342
HpyCH4III ACNGT 6 cut(s) 22, 28, 212, 347, 383, 494
HpyCH4IV ACGT 1 cut(s) 407
HpyCH4V TGCA 1 cut(s) 505
HpyF3I CTNAG 1 cut(s) 219
HpySE526I ACGT 1 cut(s) 407
Hsp92I GRCGYC 1 cut(s) 76
Hsp92II CATG 5 cut(s) 97, 133, 164, 405, 460
HspAI GCGC 1 cut(s) 99
Kzo9I GATC 4 cut(s) 9, 215, 229, 475
LmnI GCTCC 1 cut(s) 266
LpnPI CCDG 7 cut(s) 26, 101, 198, 223, 325, 378, 411
LweI GCATC 1 cut(s) 177
MaeII ACGT 1 cut(s) 407
MaeIII GTNAC 4 cut(s) 22, 28, 136, 341
MalI GATC 4 cut(s) 11, 217, 231, 477
MboI GATC 4 cut(s) 9, 215, 229, 475
MluCI AATT 5 cut(s) 40, 49, 269, 487, 514
MlyI GAGTC 1 cut(s) 295
MnlI CCTC 2 cut(s) 257, 339
MseI TTAA 1 cut(s) 174
MslI CAYNNNNRTG 2 cut(s) 134, 406
MspI CCGG 1 cut(s) 185
MspR9I CCNGG 2 cut(s) 14, 185
MvaI CCWGG 1 cut(s) 14
NciI CCSGG 1 cut(s) 185
NdeII GATC 4 cut(s) 9, 215, 229, 475
NlaIII CATG 5 cut(s) 97, 133, 164, 405, 460
NmuCI GTSAC 2 cut(s) 136, 341
NspI RCATGY 2 cut(s) 164, 405
PciI ACATGT 2 cut(s) 160, 401
PflMI CCANNNNNTGG 1 cut(s) 291
PfoI TCCNGGA 1 cut(s) 183
PkrI GCNGC 1 cut(s) 483
PleI GAGTC 1 cut(s) 295
PpsI GAGTC 1 cut(s) 295
Ppu21I YACGTR 1 cut(s) 408
PscI ACATGT 2 cut(s) 160, 401
Psp6I CCWGG 1 cut(s) 12
PspGI CCWGG 1 cut(s) 12
PspPI GGNCC 3 cut(s) 187, 282, 428
RsaI GTAC 3 cut(s) 198, 406, 413
RsaNI GTAC 3 cut(s) 197, 405, 412
RseI CAYNNNNRTG 2 cut(s) 134, 406
SaqAI TTAA 1 cut(s) 174
SatI GCNGC 1 cut(s) 482
Sau3AI GATC 4 cut(s) 9, 215, 229, 475
Sau96I GGNCC 3 cut(s) 187, 282, 428
SchI GAGTC 1 cut(s) 295
ScrFI CCNGG 2 cut(s) 14, 185
SetI ASST 5 cut(s) 90, 249, 263, 397, 410
SfaNI GCATC 1 cut(s) 177
SinI GGWCC 2 cut(s) 187, 428
SmiMI CAYNNNNRTG 2 cut(s) 134, 406
Sse9I AATT 5 cut(s) 40, 49, 269, 487, 514
SsiI CCGC 1 cut(s) 482
StyD4I CCNGG 2 cut(s) 12, 183
TaaI ACNGT 6 cut(s) 22, 28, 212, 347, 383, 494
TaiI ACGT 1 cut(s) 410
TaqI TCGA 1 cut(s) 214
TasI AATT 5 cut(s) 40, 49, 269, 487, 514
TauI GCSGC 1 cut(s) 484
Tru1I TTAA 1 cut(s) 174
Tru9I TTAA 1 cut(s) 174
TscAI CASTG 1 cut(s) 215
TseFI GTSAC 2 cut(s) 136, 341
Tsp45I GTSAC 2 cut(s) 136, 341
TspDTI ATGAA 4 cut(s) 118, 282, 335, 458
TspGWI ACGGA 3 cut(s) 124, 193, 420
TspRI CASTG 1 cut(s) 215
Van91I CCANNNNNTGG 1 cut(s) 291
VpaK11BI GGWCC 2 cut(s) 187, 428
XapI RAATTY 1 cut(s) 269
XceI RCATGY 2 cut(s) 164, 405
XcmI CCANNNNNNNNNTGG 2 cut(s) 463, 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.