RLG00000010327

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0001549
Physical Location & Seq
Reverse (-)
680977 .. 683331
2355 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010327

Sequence Viewer

Length: 549 bp
ATGTTGAAAGCCAAAACACATGTTCGCATCATTAACAATTTACCGGACGACGTGCAACTAAACATCCATTGTCAATCCGGGGACGATGATCTTGGCTTCCATGAACTCTCCTACCTCAGCTCTTTCGAATTTAGTTTCAACCCGAACTGGTTTGGGACGACTCGATTTTTTTGCTTCATGGTGTGGCTCTCCAATGGTCACTATTTTGATATCTACGAGTACCGGAGAGATGCTAATACTGAGTTTAGCCCAAGTGGTGAGAGGCGAGGTCCGGCGACCAACATTGGTGCTATTTTGGTGGCTATTGAGCCGTGTATTTCCGATATTTACGCTCTACACGCATGTGGTATAAACAAATCCCCCAAGTCCCCAGTTAAGAGTTCTCCTGAGTGGAGATTCCCCCCCTCGGGTCCCCCAATTTTCCGGTCAAGAGAATTTGAAGGCCGAGTCTCCCCTGGTCCCGAGTTTTCCCGGAGTGTTGTCGGTACCATTGAGAACACTTGGCTCATGTTCGCTATTTATGTGATTTCCACTACTTGTGGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

183

Amino Acids

20.57

Weight (kDa)

5.94

Isoelectric Point (pI)

61.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 7 - 79 5.3e-20 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 485
AccB1I GGYRCC 1 cut(s) 485
AcsI RAATTY 2 cut(s) 128, 434
AfaI GTAC 2 cut(s) 221, 487
AfiI CCNNNNNNNGG 2 cut(s) 406, 407
AflIII ACRYGT 1 cut(s) 19
AgsI TTSAA 3 cut(s) 7, 139, 440
AjiI CACGTC 1 cut(s) 52
AjnI CCWGG 1 cut(s) 454
AleI CACNNNNGTG 1 cut(s) 342
AluBI AGCT 1 cut(s) 120
AluI AGCT 1 cut(s) 120
Alw26I GTCTC 1 cut(s) 454
Ama87I CYCGRG 2 cut(s) 406, 461
AoxI GGCC 1 cut(s) 442
ApoI RAATTY 2 cut(s) 128, 434
Asp718I GGTACC 1 cut(s) 485
AspS9I GGNCC 3 cut(s) 269, 410, 458
AsuC2I CCSGG 2 cut(s) 79, 472
AsuHPI GGTGA 1 cut(s) 269
AsuII TTCGAA 1 cut(s) 126
AvaI CYCGRG 2 cut(s) 406, 461
AvaII GGWCC 3 cut(s) 269, 410, 458
BanI GGYRCC 1 cut(s) 485
BbvCI CCTCAGC 1 cut(s) 116
BceAI ACGGC 1 cut(s) 295
BcgI CGANNNNNNTGC 2 cut(s) 153, 187
BciT130I CCWGG 1 cut(s) 456
BcnI CCSGG 2 cut(s) 79, 472
BcoDI GTCTC 1 cut(s) 454
Bme1390I CCNGG 3 cut(s) 79, 456, 472
Bme18I GGWCC 3 cut(s) 269, 410, 458
BmeT110I CYCGRG 2 cut(s) 406, 461
BmgBI CACGTC 1 cut(s) 52
BmgT120I GGNCC 3 cut(s) 269, 410, 458
BmiI GGNNCC 4 cut(s) 411, 412, 460, 487
BmrFI CCNGG 3 cut(s) 79, 456, 472
BmrI ACTGGG 1 cut(s) 365
BmsI GCATC 2 cut(s) 36, 220
BmuI ACTGGG 1 cut(s) 365
Bpu10I CCTNAGC 1 cut(s) 116
Bpu14I TTCGAA 1 cut(s) 126
BpuMI CCSGG 2 cut(s) 79, 472
BsaJI CCNNGG 3 cut(s) 78, 405, 454
BsaWI WCCGGW 3 cut(s) 43, 222, 423
Bsc4I CCNNNNNNNGG 2 cut(s) 406, 407
Bse1I ACTGG 2 cut(s) 152, 371
BseBI CCWGG 1 cut(s) 456
BseDI CCNNGG 3 cut(s) 78, 405, 454
BseGI GGATG 1 cut(s) 63
BseLI CCNNNNNNNGG 2 cut(s) 406, 407
BseMII CTCAG 3 cut(s) 130, 231, 378
BseNI ACTGG 2 cut(s) 152, 371
BshFI GGCC 1 cut(s) 444
BshNI GGYRCC 1 cut(s) 485
BsiHKCI CYCGRG 2 cut(s) 406, 461
BsiSI CCGG 6 cut(s) 44, 78, 223, 272, 424, 472
BslFI GGGAC 5 cut(s) 95, 169, 352, 396, 444
BslI CCNNNNNNNGG 2 cut(s) 406, 407
BsmAI GTCTC 1 cut(s) 454
BsmFI GGGAC 5 cut(s) 95, 169, 352, 396, 444
BsnI GGCC 1 cut(s) 444
BsoBI CYCGRG 2 cut(s) 406, 461
Bsp119I TTCGAA 1 cut(s) 126
Bsp143I GATC 1 cut(s) 88
BspANI GGCC 1 cut(s) 444
BspCNI CTCAG 3 cut(s) 129, 232, 379
BspLI GGNNCC 4 cut(s) 411, 412, 460, 487
BspT104I TTCGAA 1 cut(s) 126
BspT107I GGYRCC 1 cut(s) 485
BsrI ACTGG 2 cut(s) 152, 371
BssECI CCNNGG 3 cut(s) 78, 405, 454
BssMI GATC 1 cut(s) 88
Bst2UI CCWGG 1 cut(s) 456
BstBI TTCGAA 1 cut(s) 126
BstDEI CTNAG 3 cut(s) 116, 240, 387
BstF5I GGATG 1 cut(s) 63
BstKTI GATC 1 cut(s) 91
BstMAI GTCTC 1 cut(s) 454
BstMBI GATC 1 cut(s) 88
BstMWI GCNNNNNNNGC 1 cut(s) 338
BstNI CCWGG 1 cut(s) 456
BstNSI RCATGY 2 cut(s) 23, 345
BstSCI CCNGG 3 cut(s) 77, 454, 470
BsuRI GGCC 1 cut(s) 444
BtrI CACGTC 1 cut(s) 52
BtsCI GGATG 1 cut(s) 63
Cfr13I GGNCC 3 cut(s) 269, 410, 458
Csp6I GTAC 2 cut(s) 220, 486
CviAII CATG 5 cut(s) 20, 101, 178, 342, 508
CviJI RGCY 9 cut(s) 11, 96, 120, 187, 249, 302, 310, 444, 505
CviKI_1 RGCY 9 cut(s) 11, 96, 120, 187, 249, 302, 310, 444, 505
CviQI GTAC 2 cut(s) 220, 486
DdeI CTNAG 3 cut(s) 116, 240, 387
DpnI GATC 1 cut(s) 90
DpnII GATC 1 cut(s) 88
Eco32I GATATC 1 cut(s) 211
Eco47I GGWCC 3 cut(s) 269, 410, 458
Eco88I CYCGRG 2 cut(s) 406, 461
EcoO109I RGGNCCY 1 cut(s) 410
EcoRII CCWGG 1 cut(s) 454
EcoRV GATATC 1 cut(s) 211
FaeI CATG 5 cut(s) 23, 104, 181, 345, 511
FaiI YATR 7 cut(s) 21, 102, 179, 343, 350, 509, 522
FaqI GGGAC 5 cut(s) 95, 169, 352, 396, 444
FatI CATG 5 cut(s) 19, 100, 177, 341, 507
FokI GGATG 1 cut(s) 50
HaeIII GGCC 1 cut(s) 444
HapII CCGG 6 cut(s) 44, 78, 223, 272, 424, 472
Hin1II CATG 5 cut(s) 23, 104, 181, 345, 511
HinfI GANTC 3 cut(s) 160, 396, 447
HpaII CCGG 6 cut(s) 44, 78, 223, 272, 424, 472
HphI GGTGA 1 cut(s) 269
Hpy188I TCNGA 1 cut(s) 322
Hpy188III TCNNGA 4 cut(s) 386, 429, 461, 546
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 1 cut(s) 434
HpyCH4IV ACGT 1 cut(s) 51
HpyCH4V TGCA 1 cut(s) 55
HpyF10VI GCNNNNNNNGC 1 cut(s) 338
HpyF3I CTNAG 3 cut(s) 116, 240, 387
HpySE526I ACGT 1 cut(s) 51
Hsp92II CATG 5 cut(s) 23, 104, 181, 345, 511
KflI GGGWCCC 1 cut(s) 410
KpnI GGTACC 1 cut(s) 489
Kzo9I GATC 1 cut(s) 88
LweI GCATC 2 cut(s) 36, 220
MaeII ACGT 1 cut(s) 51
MaeIII GTNAC 1 cut(s) 197
MalI GATC 1 cut(s) 90
MboI GATC 1 cut(s) 88
MluCI AATT 4 cut(s) 37, 128, 417, 434
MlyI GAGTC 2 cut(s) 154, 456
MnlI CCTC 4 cut(s) 125, 255, 260, 415
MseI TTAA 2 cut(s) 33, 375
MslI CAYNNNNRTG 1 cut(s) 342
MspI CCGG 6 cut(s) 44, 78, 223, 272, 424, 472
MspR9I CCNGG 3 cut(s) 79, 456, 472
MvaI CCWGG 1 cut(s) 456
MwoI GCNNNNNNNGC 1 cut(s) 338
NciI CCSGG 2 cut(s) 79, 472
NdeII GATC 1 cut(s) 88
NlaIII CATG 5 cut(s) 23, 104, 181, 345, 511
NlaIV GGNNCC 4 cut(s) 411, 412, 460, 487
NmeAIII GCCGAG 1 cut(s) 470
NmuCI GTSAC 1 cut(s) 197
NspI RCATGY 2 cut(s) 23, 345
NspV TTCGAA 1 cut(s) 126
OliI CACNNNNGTG 1 cut(s) 342
PciI ACATGT 1 cut(s) 19
PfeI GAWTC 1 cut(s) 396
PfoI TCCNGGA 1 cut(s) 470
PleI GAGTC 2 cut(s) 154, 455
PpsI GAGTC 2 cut(s) 154, 455
PpuMI RGGWCCY 1 cut(s) 410
PscI ACATGT 1 cut(s) 19
Psp5II RGGWCCY 1 cut(s) 410
Psp6I CCWGG 1 cut(s) 454
PspGI CCWGG 1 cut(s) 454
PspN4I GGNNCC 4 cut(s) 411, 412, 460, 487
PspPI GGNCC 3 cut(s) 269, 410, 458
PspPPI RGGWCCY 1 cut(s) 410
RsaI GTAC 2 cut(s) 221, 487
RsaNI GTAC 2 cut(s) 220, 486
RseI CAYNNNNRTG 1 cut(s) 342
SaqAI TTAA 2 cut(s) 33, 375
Sau3AI GATC 1 cut(s) 88
Sau96I GGNCC 3 cut(s) 269, 410, 458
SchI GAGTC 2 cut(s) 154, 456
ScrFI CCNGG 3 cut(s) 79, 456, 472
SetI ASST 4 cut(s) 54, 117, 122, 271
SfaNI GCATC 2 cut(s) 36, 220
SfuI TTCGAA 1 cut(s) 126
SinI GGWCC 3 cut(s) 269, 410, 458
SmiMI CAYNNNNRTG 1 cut(s) 342
Sse9I AATT 4 cut(s) 37, 128, 417, 434
StyD4I CCNGG 3 cut(s) 77, 454, 470
TaiI ACGT 1 cut(s) 54
TaqI TCGA 2 cut(s) 126, 163
TasI AATT 4 cut(s) 37, 128, 417, 434
TfiI GAWTC 1 cut(s) 396
Tru1I TTAA 2 cut(s) 33, 375
Tru9I TTAA 2 cut(s) 33, 375
TseFI GTSAC 1 cut(s) 197
Tsp45I GTSAC 1 cut(s) 197
TspDTI ATGAA 2 cut(s) 117, 166
VpaK11BI GGWCC 3 cut(s) 269, 410, 458
XapI RAATTY 2 cut(s) 128, 434
XceI RCATGY 2 cut(s) 23, 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.