FvH4_3g02310

DNA binding domain with preference for A/T rich regions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001372
Physical Location & Seq
Reverse (-)
1170407 .. 1174072
3666 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g02310.t1

Sequence Viewer

Length: 1536 bp
ATGAATCCAAATCAAGGTGCAAGCTCGTTATCTTCACCAGATCTCCCTATGAAGCGGAAACGTGGACGTCCACGTAAGGATGAGAATTTAGTTCATGGAGAGAGCATGCCTGCAATACCAGGACCTGATAGTCTGAAGAAAAACAAGCAGAGTGCGGGTACTAACGATGATGCTGAAGGTGAAATGGTGGGTCGGGTGGTTTCTGGTGTTATTGAAGGTTCATTTGATGCCGGCTATCTTCTTAATGTTAAGGTAGAAGACACCGACACTCAGCTGAGGGGTGTTGTATTTCTACCGGGAAAGTTCACTCCAGTTACTGCAGCAAATGATGTTGCTCCAAATGTTAAGATGTGCAGAAGAAAAGAAGTTCCAATACCAGCTGTGACCATGCAATCTCATCTTTATGGTTCTGCCCAGCCATCAGAGCAAAGTAACAAGCAACCTGTTGAGGTGAAAAAGCAAATACCTGCAGTTCCAGACCAAATTCCACCCTGTATTCCTCAGTGTGGCATCACGGAGTCTCTTAGAAGTCAATCTTTCTCTGGCATCCCGGAGACTCTTAGAAGCCAATGCATCCAGAAGTCTTTTAGAAGTCAATCTTTCTCTGGCATCCCAGAGTCATCCTCTTTTGTGACTCCTCCGGTTGATAATTTGCCAAAGAATGATGCCCCTACCTTGGGAGGTAAAGCCATACCACAGCAACCTTTGGCGTCTGTACTTGATGACCAATCCACCCATGTAACGACAAAGTCCGAGAATGATGAAGTTGTTGAACAGGAAAAAGTGTTGGTGGAATTTGAACCGGCCACAACAATAACAGAGCCAACTGGTGTAGAGGACAATAAAGAATCAAAAACAGAAAAATTATTGGAGGAATCATCTGAACCAATTGGTGTTGTACCAAGCATAGAAACAGTAAGTAAGGATCCTCCAATTCAACCTCAGGTTGTGGGTTCTGAAACCAGTGCATTGGTTCAGGATGATGTAAAGAGCCTGGATTTTGAACTCAACCATACTCCTTTATTTGCTGAAGCTGCTCCTGCAATGGCTACCGTACCAAGTGTTGAAACTGTATGCAAGGCTCCTCATATTCAACCTCAGACTGTGGATACTGAAAAGAGTCAATTGGTTGACGATGAGGTAAAGAGTGTGAACGTTGAAATAAATCAAACTCCTGTATTTGCTGAATCGGCACCAGGCTCTGTCATACTGGGAGCTGAGACTGTATGTAAGGATCCTCAAATTCAACCTGAGGATGTGGGTTCTGAAAAGAGTACATTGGTTCAGGATGTGGTAAAGAGCCTAGATCTTGAAATCAACCAAACTCCCCTAGTTGCAGAACCTGCAATTGCTGCCAGTAAACCAGTTGAGAATCTGATGGAAGAAGAGGCTACACCGAAGCCGAGTCAGGTGTTAGGGGGAGAGACTGTTGCATCTGTAGGATCCATTCCTTCAGCCATGGGTGAACCTCCTTCTGGTGCTATTGACAGTATGGAATGTGACATTGCAGATGCCATTCCACCTGCACAATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

512

Amino Acids

54.29

Weight (kDa)

4.51

Isoelectric Point (pI)

58.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1531
AasI GACNNNNNNGTC 1 cut(s) 129
AatII GACGTC 1 cut(s) 70
Acc36I ACCTGC 3 cut(s) 475, 1351, 1531
AccB1I GGYRCC 1 cut(s) 1192
AciI CCGC 2 cut(s) 55, 155
AclI AACGTT 1 cut(s) 1155
AclWI GGATC 6 cut(s) 920, 933, 1229, 1242, 1437, 1450
AcoI YGGCCR 1 cut(s) 804
AcsI RAATTY 4 cut(s) 85, 483, 794, 1242
AcuI CTGAAG 4 cut(s) 155, 195, 1050, 1437
AcyI GRCGYC 2 cut(s) 67, 710
AfaI GTAC 5 cut(s) 160, 717, 900, 1056, 1276
AfiI CCNNNNNNNGG 5 cut(s) 14, 506, 676, 677, 1475
AjnI CCWGG 3 cut(s) 118, 993, 1195
AluBI AGCT 5 cut(s) 24, 274, 380, 1034, 1217
AluI AGCT 5 cut(s) 24, 274, 380, 1034, 1217
Alw26I GTCTC 4 cut(s) 525, 548, 1214, 1418
AlwI GGATC 6 cut(s) 920, 933, 1229, 1242, 1437, 1450
AlwNI CAGNNNCTG 4 cut(s) 125, 317, 1202, 1343
AoxI GGCC 1 cut(s) 804
ApeKI GCWGC 3 cut(s) 320, 1034, 1352
ApoI RAATTY 4 cut(s) 85, 483, 794, 1242
AspS9I GGNCC 1 cut(s) 122
AsuC2I CCSGG 2 cut(s) 297, 551
AsuHPI GGTGA 4 cut(s) 27, 191, 463, 1475
AvaII GGWCC 1 cut(s) 122
AxyI CCTNAGG 2 cut(s) 942, 1251
BamHI GGATCC 3 cut(s) 925, 1234, 1442
BanI GGYRCC 1 cut(s) 1192
BarI GAAGNNNNNNTAC 2 cut(s) 357, 389
BbsI GAAGAC 1 cut(s) 264
BbvCI CCTCAGC 1 cut(s) 275
BbvI GCAGC 3 cut(s) 332, 1021, 1339
BccI CCATC 2 cut(s) 427, 1372
BciT130I CCWGG 3 cut(s) 120, 995, 1197
BciVI GTATCC 1 cut(s) 1102
BcnI CCSGG 2 cut(s) 297, 551
BcoDI GTCTC 4 cut(s) 525, 548, 1214, 1418
BfaI CTAG 2 cut(s) 1304, 1331
BfmI CTRYAG 3 cut(s) 318, 468, 1437
BfuAI ACCTGC 3 cut(s) 475, 1351, 1531
BfuI GTATCC 1 cut(s) 1102
BglII AGATCT 2 cut(s) 40, 1306
BisI GCNGC 3 cut(s) 321, 1035, 1353
BlsI GCNGC 3 cut(s) 322, 1036, 1354
Bme1390I CCNGG 5 cut(s) 120, 297, 551, 995, 1197
Bme18I GGWCC 1 cut(s) 122
BmgT120I GGNCC 1 cut(s) 122
BmiI GGNNCC 5 cut(s) 927, 1083, 1194, 1236, 1444
BmrFI CCNGG 5 cut(s) 120, 297, 551, 995, 1197
BmrI ACTGGG 1 cut(s) 1220
BmsI GCATC 9 cut(s) 160, 217, 519, 555, 582, 618, 655, 1442, 1501
BmuI ACTGGG 1 cut(s) 1220
BpiI GAAGAC 1 cut(s) 264
BplI GAGNNNNNCTC 2 cut(s) 608, 640
BpmI CTGGAG 1 cut(s) 294
Bpu10I CCTNAGC 1 cut(s) 275
BpuMI CCSGG 2 cut(s) 297, 551
BsaAI YACGTR 1 cut(s) 74
BsaHI GRCGYC 2 cut(s) 67, 710
BsaJI CCNNGG 2 cut(s) 675, 1458
BsaWI WCCGGW 1 cut(s) 640
BsaXI ACNNNNNCTCC 4 cut(s) 27, 57, 1413, 1443
Bsc4I CCNNNNNNNGG 5 cut(s) 14, 506, 676, 677, 1475
Bse118I RCCGGY 2 cut(s) 230, 802
Bse1I ACTGG 6 cut(s) 311, 832, 963, 1215, 1356, 1364
Bse21I CCTNAGG 2 cut(s) 942, 1251
Bse3DI GCAATG 2 cut(s) 1050, 1503
BseBI CCWGG 3 cut(s) 120, 995, 1197
BseDI CCNNGG 2 cut(s) 675, 1458
BseGI GGATG 8 cut(s) 85, 546, 573, 609, 620, 985, 1261, 1294
BseLI CCNNNNNNNGG 5 cut(s) 14, 506, 676, 677, 1475
BseMI GCAATG 2 cut(s) 1050, 1503
BseMII CTCAG 7 cut(s) 266, 284, 515, 956, 1112, 1209, 1242
BseNI ACTGG 6 cut(s) 311, 832, 963, 1215, 1356, 1364
BseRI GAGGAG 2 cut(s) 627, 1074
BseXI GCAGC 3 cut(s) 332, 1021, 1339
BseYI CCCAGC 1 cut(s) 414
BsgI GTGCAG 2 cut(s) 373, 1509
BshFI GGCC 1 cut(s) 806
BshNI GGYRCC 1 cut(s) 1192
BsiSI CCGG 5 cut(s) 231, 296, 551, 641, 803
BslI CCNNNNNNNGG 5 cut(s) 14, 506, 676, 677, 1475
BsmAI GTCTC 4 cut(s) 525, 548, 1214, 1418
BsnI GGCC 1 cut(s) 806
Bsp143I GATC 5 cut(s) 40, 925, 1234, 1306, 1442
Bsp19I CCATGG 1 cut(s) 1458
BspACI CCGC 2 cut(s) 55, 155
BspANI GGCC 1 cut(s) 806
BspCNI CTCAG 7 cut(s) 267, 283, 514, 955, 1111, 1210, 1243
BspLI GGNNCC 5 cut(s) 927, 1083, 1194, 1236, 1444
BspMAI CTGCAG 2 cut(s) 322, 472
BspMI ACCTGC 3 cut(s) 475, 1351, 1531
BspPI GGATC 6 cut(s) 920, 933, 1229, 1242, 1437, 1450
BspT107I GGYRCC 1 cut(s) 1192
BsrDI GCAATG 2 cut(s) 1050, 1503
BsrFI RCCGGY 2 cut(s) 230, 802
BsrI ACTGG 6 cut(s) 311, 832, 963, 1215, 1356, 1364
BssAI RCCGGY 2 cut(s) 230, 802
BssECI CCNNGG 2 cut(s) 675, 1458
BssMI GATC 5 cut(s) 40, 925, 1234, 1306, 1442
BssNI GRCGYC 2 cut(s) 67, 710
BssT1I CCWWGG 2 cut(s) 675, 1458
Bst2UI CCWGG 3 cut(s) 120, 995, 1197
Bst4CI ACNGT 7 cut(s) 916, 1054, 1072, 1105, 1225, 1429, 1490
Bst6I CTCTTC 1 cut(s) 1380
BstACI GRCGYC 2 cut(s) 67, 710
BstAPI GCANNNNNTGC 2 cut(s) 1343, 1352
BstBAI YACGTR 1 cut(s) 74
BstC8I GCNNGC 4 cut(s) 22, 107, 111, 232
BstDSI CCRYGG 1 cut(s) 1458
BstF5I GGATG 8 cut(s) 85, 546, 573, 609, 620, 985, 1261, 1294
BstKTI GATC 5 cut(s) 43, 928, 1237, 1309, 1445
BstMAI GTCTC 4 cut(s) 525, 548, 1214, 1418
BstMBI GATC 5 cut(s) 40, 925, 1234, 1306, 1442
BstMWI GCNNNNNNNGC 6 cut(s) 424, 1034, 1040, 1190, 1343, 1352
BstNI CCWGG 3 cut(s) 120, 995, 1197
BstNSI RCATGY 1 cut(s) 109
BstSCI CCNGG 5 cut(s) 118, 295, 549, 993, 1195
BstSFI CTRYAG 3 cut(s) 318, 468, 1437
BstV1I GCAGC 3 cut(s) 332, 1021, 1339
BstV2I GAAGAC 1 cut(s) 264
BstX2I RGATCY 5 cut(s) 40, 925, 1234, 1306, 1442
BstXI CCANNNNNNTGG 1 cut(s) 970
BstYI RGATCY 5 cut(s) 40, 925, 1234, 1306, 1442
Bsu36I CCTNAGG 2 cut(s) 942, 1251
BsuI GTATCC 1 cut(s) 1102
BsuRI GGCC 1 cut(s) 806
BtgI CCRYGG 1 cut(s) 1458
BtsCI GGATG 8 cut(s) 85, 546, 573, 609, 620, 985, 1261, 1294
BtsIMutI CAGTG 2 cut(s) 509, 970
BveI ACCTGC 3 cut(s) 475, 1351, 1531
Cac8I GCNNGC 4 cut(s) 22, 107, 111, 232
CaiI CAGNNNCTG 4 cut(s) 125, 317, 1202, 1343
Cfr10I RCCGGY 2 cut(s) 230, 802
Cfr13I GGNCC 1 cut(s) 122
CseI GACGC 1 cut(s) 699
Csp6I GTAC 5 cut(s) 159, 716, 899, 1055, 1275
CviAII CATG 5 cut(s) 95, 106, 388, 737, 1459
CviQI GTAC 5 cut(s) 159, 716, 899, 1055, 1275
DpnI GATC 5 cut(s) 42, 927, 1236, 1308, 1444
DpnII GATC 5 cut(s) 40, 925, 1234, 1306, 1442
DrdI GACNNNNNNGTC 1 cut(s) 129
DseDI GACNNNNNNGTC 1 cut(s) 129
EaeI YGGCCR 1 cut(s) 804
Eam1104I CTCTTC 1 cut(s) 1380
EarI CTCTTC 1 cut(s) 1380
Eco130I CCWWGG 2 cut(s) 675, 1458
Eco47I GGWCC 1 cut(s) 122
Eco57I CTGAAG 4 cut(s) 155, 195, 1050, 1437
Eco81I CCTNAGG 2 cut(s) 942, 1251
EcoO109I RGGNCCY 1 cut(s) 122
EcoRII CCWGG 3 cut(s) 118, 993, 1195
EcoT14I CCWWGG 2 cut(s) 675, 1458
EcoT22I ATGCAT 1 cut(s) 575
ErhI CCWWGG 2 cut(s) 675, 1458
FaeI CATG 5 cut(s) 98, 109, 391, 740, 1462
FalI AAGNNNNNCTT 4 cut(s) 520, 552, 583, 615
FatI CATG 5 cut(s) 94, 105, 387, 736, 1458
FauI CCCGC 1 cut(s) 148
Fnu4HI GCNGC 3 cut(s) 321, 1035, 1353
FokI GGATG 8 cut(s) 92, 533, 560, 596, 607, 992, 1268, 1301
Fsp4HI GCNGC 3 cut(s) 321, 1035, 1353
FspBI CTAG 2 cut(s) 1304, 1331
GluI GCNGC 3 cut(s) 321, 1035, 1353
GsaI CCCAGC 1 cut(s) 418
GsuI CTGGAG 1 cut(s) 294
HaeIII GGCC 1 cut(s) 806
HapII CCGG 5 cut(s) 231, 296, 551, 641, 803
HgaI GACGC 1 cut(s) 699
Hin1I GRCGYC 2 cut(s) 67, 710
Hin1II CATG 5 cut(s) 98, 109, 391, 740, 1462
HincII GTYRAC 1 cut(s) 1132
HindII GTYRAC 1 cut(s) 1132
HpaII CCGG 5 cut(s) 231, 296, 551, 641, 803
HphI GGTGA 4 cut(s) 27, 191, 463, 1475
Hpy166II GTNNAC 7 cut(s) 65, 71, 306, 1132, 1153, 1361, 1466
Hpy188I TCNGA 8 cut(s) 135, 424, 754, 883, 958, 1101, 1267, 1377
Hpy188III TCNNGA 5 cut(s) 476, 577, 977, 1286, 1310
Hpy8I GTNNAC 7 cut(s) 65, 71, 306, 1132, 1153, 1361, 1466
HpyAV CCTTC 4 cut(s) 170, 209, 1461, 1482
HpyCH4III ACNGT 7 cut(s) 916, 1054, 1072, 1105, 1225, 1429, 1490
HpyCH4IV ACGT 4 cut(s) 61, 67, 73, 1155
HpyF10VI GCNNNNNNNGC 6 cut(s) 424, 1034, 1040, 1190, 1343, 1352
HpySE526I ACGT 4 cut(s) 61, 67, 73, 1155
Hsp92I GRCGYC 2 cut(s) 67, 710
Hsp92II CATG 5 cut(s) 98, 109, 391, 740, 1462
KroI GCCGGC 1 cut(s) 230
KroNI GCCGGC 1 cut(s) 232
Kzo9I GATC 5 cut(s) 40, 925, 1234, 1306, 1442
LmnI GCTCC 4 cut(s) 340, 1042, 1087, 1214
Lsp1109I GCAGC 3 cut(s) 332, 1021, 1339
LweI GCATC 9 cut(s) 160, 217, 519, 555, 582, 618, 655, 1442, 1501
MaeI CTAG 2 cut(s) 1304, 1331
MaeII ACGT 4 cut(s) 61, 67, 73, 1155
MaeIII GTNAC 6 cut(s) 313, 382, 431, 631, 739, 1499
MalI GATC 5 cut(s) 42, 927, 1236, 1308, 1444
MboI GATC 5 cut(s) 40, 925, 1234, 1306, 1442
MboII GAAGA 7 cut(s) 24, 148, 230, 269, 369, 1394, 1397
MfeI CAATTG 3 cut(s) 888, 1124, 1347
MflI RGATCY 5 cut(s) 40, 925, 1234, 1306, 1442
MlyI GAGTC 6 cut(s) 527, 550, 626, 628, 1129, 1414
Mph1103I ATGCAT 1 cut(s) 575
MroNI GCCGGC 1 cut(s) 230
MseI TTAA 3 cut(s) 243, 249, 345
MslI CAYNNNNRTG 1 cut(s) 402
MspA1I CMGCKG 2 cut(s) 274, 380
MspI CCGG 5 cut(s) 231, 296, 551, 641, 803
MspR9I CCNGG 5 cut(s) 120, 297, 551, 995, 1197
MunI CAATTG 3 cut(s) 888, 1124, 1347
MvaI CCWGG 3 cut(s) 120, 995, 1197
MwoI GCNNNNNNNGC 6 cut(s) 424, 1034, 1040, 1190, 1343, 1352
NaeI GCCGGC 1 cut(s) 232
NciI CCSGG 2 cut(s) 297, 551
NcoI CCATGG 1 cut(s) 1458
NdeII GATC 5 cut(s) 40, 925, 1234, 1306, 1442
NgoMIV GCCGGC 1 cut(s) 230
NlaIII CATG 5 cut(s) 98, 109, 391, 740, 1462
NlaIV GGNNCC 5 cut(s) 927, 1083, 1194, 1236, 1444
NmeAIII GCCGAG 1 cut(s) 1428
NmuCI GTSAC 3 cut(s) 382, 631, 1499
NsiI ATGCAT 1 cut(s) 575
NspI RCATGY 1 cut(s) 109
PaeI GCATGC 1 cut(s) 109
PaqCI CACCTGC 1 cut(s) 1531
PdiI GCCGGC 1 cut(s) 232
PfeI GAWTC 5 cut(s) 4, 848, 875, 1187, 1372
PflFI GACNNNGTC 1 cut(s) 748
PfoI TCCNGGA 1 cut(s) 549
PkrI GCNGC 3 cut(s) 322, 1036, 1354
PleI GAGTC 6 cut(s) 526, 550, 625, 628, 1128, 1413
PpsI GAGTC 6 cut(s) 526, 550, 625, 628, 1128, 1413
Ppu21I YACGTR 1 cut(s) 74
PpuMI RGGWCCY 1 cut(s) 122
Psp1406I AACGTT 1 cut(s) 1155
Psp5II RGGWCCY 1 cut(s) 122
Psp6I CCWGG 3 cut(s) 118, 993, 1195
PspFI CCCAGC 1 cut(s) 414
PspGI CCWGG 3 cut(s) 118, 993, 1195
PspN4I GGNNCC 5 cut(s) 927, 1083, 1194, 1236, 1444
PspPI GGNCC 1 cut(s) 122
PspPPI RGGWCCY 1 cut(s) 122
PstI CTGCAG 2 cut(s) 322, 472
PstNI CAGNNNCTG 4 cut(s) 125, 317, 1202, 1343
PsuI RGATCY 5 cut(s) 40, 925, 1234, 1306, 1442
PsyI GACNNNGTC 1 cut(s) 748
PvuII CAGCTG 2 cut(s) 274, 380
RsaI GTAC 5 cut(s) 160, 717, 900, 1056, 1276
RsaNI GTAC 5 cut(s) 159, 716, 899, 1055, 1275
RseI CAYNNNNRTG 1 cut(s) 402
SaqAI TTAA 3 cut(s) 243, 249, 345
SatI GCNGC 3 cut(s) 321, 1035, 1353
Sau3AI GATC 5 cut(s) 40, 925, 1234, 1306, 1442
Sau96I GGNCC 1 cut(s) 122
SchI GAGTC 6 cut(s) 527, 550, 626, 628, 1129, 1414
ScrFI CCNGG 5 cut(s) 120, 297, 551, 995, 1197
SfaNI GCATC 9 cut(s) 160, 217, 519, 555, 582, 618, 655, 1442, 1501
SfcI CTRYAG 3 cut(s) 318, 468, 1437
SinI GGWCC 1 cut(s) 122
SmiMI CAYNNNNRTG 1 cut(s) 402
SphI GCATGC 1 cut(s) 109
SsiI CCGC 2 cut(s) 55, 155
SspMI CTAG 2 cut(s) 1304, 1331
StyD4I CCNGG 5 cut(s) 118, 295, 549, 993, 1195
StyI CCWWGG 2 cut(s) 675, 1458
TaaI ACNGT 7 cut(s) 916, 1054, 1072, 1105, 1225, 1429, 1490
TaiI ACGT 4 cut(s) 64, 70, 76, 1158
TatI WGTACW 2 cut(s) 715, 1274
TfiI GAWTC 5 cut(s) 4, 848, 875, 1187, 1372
Tru1I TTAA 3 cut(s) 243, 249, 345
Tru9I TTAA 3 cut(s) 243, 249, 345
TscAI CASTG 2 cut(s) 509, 970
TseFI GTSAC 3 cut(s) 382, 631, 1499
TseI GCWGC 3 cut(s) 320, 1034, 1352
Tsp45I GTSAC 3 cut(s) 382, 631, 1499
TspDTI ATGAA 5 cut(s) 17, 65, 83, 210, 777
TspGWI ACGGA 1 cut(s) 530
TspRI CASTG 2 cut(s) 509, 970
Tth111I GACNNNGTC 1 cut(s) 748
VpaK11BI GGWCC 1 cut(s) 122
XapI RAATTY 4 cut(s) 85, 483, 794, 1242
XceI RCATGY 1 cut(s) 109
XspI CTAG 2 cut(s) 1304, 1331
ZraI GACGTC 1 cut(s) 68
Zsp2I ATGCAT 1 cut(s) 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.