Rh5CG032800

DNA binding domain with preference for A/T rich regions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0001372
Physical Location & Seq
Forward (+)
2121121 .. 2122212
1092 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG032800.1

Sequence Viewer

Length: 669 bp
ATGCTCCACGACTTTCTTAAGTTGCCTCCTATTGCACCGGATGATGAACGTGCTTATTTTGAAGTTGGGCAGAACATAATCCCTACTTCCCATAATTCTGAAGTTAAGGCGGGTGGTGGTAAACTTTTAATTGCTTTGGATAATGCCAATCATTCCATCCGCTTTACCACCTTGATATATTCCAAGATTGATTACTACACTCGCACAACTACCTATATTGATGCTTGCTTCAGGACTCTTCGTGATGAGTTGGTGCTTCACATATCTAACTTTGGGGTTCCTTCGACCACTTCTCATGGTGGAGAGGATTTCAACATGAATATTGATGGAGAGGACCATGGAGGTTACAGTGAACAGAGTCAGTTGGTTGATGATGAGGTCAGGAGCCCGAACCTTGAACTTAATCGAACTCCTGTATTTGGTGGCCATGCACCAAGTTCTATCATGCTGGGAGGTGAGACTGTGTGCAAGGATCCTCAAATTCAACCTGATGATGTAGATTCTGAAAAGAGTGCATTGGTTCAGGATGTGATAAAGAACCTCGATCTCGAACTCAACCTATTTGCAGAACCTACATTCGCAGCTCGTGATCCAACCAGTATAGAAGAAGAGCCGGCACTAAAGCCAAGCGAGGTGTTCGGGGGAGAGACTGTTGCATCTGAACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

222

Amino Acids

24.41

Weight (kDa)

4.44

Isoelectric Point (pI)

48.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 110, 160
AclWI GGATC 3 cut(s) 467, 480, 584
AcoI YGGCCR 1 cut(s) 424
AcsI RAATTY 1 cut(s) 480
AcuI CTGAAG 2 cut(s) 120, 214
AfiI CCNNNNNNNGG 1 cut(s) 419
AflII CTTAAG 1 cut(s) 17
AgsI TTSAA 4 cut(s) 62, 313, 398, 485
AluBI AGCT 1 cut(s) 584
AluI AGCT 1 cut(s) 584
Alw26I GTCTC 2 cut(s) 452, 641
AlwI GGATC 3 cut(s) 467, 480, 584
AoxI GGCC 1 cut(s) 424
ApeKI GCWGC 1 cut(s) 581
ApoI RAATTY 1 cut(s) 480
AspS9I GGNCC 1 cut(s) 334
AsuHPI GGTGA 1 cut(s) 467
AvaII GGWCC 1 cut(s) 334
BalI TGGCCA 1 cut(s) 426
BamHI GGATCC 1 cut(s) 472
BanII GRGCYC 1 cut(s) 389
BauI CACGAG 1 cut(s) 585
BbvI GCAGC 1 cut(s) 593
BccI CCATC 2 cut(s) 164, 320
BcoDI GTCTC 2 cut(s) 452, 641
BfrI CTTAAG 1 cut(s) 17
BisI GCNGC 1 cut(s) 582
BlsI GCNGC 1 cut(s) 583
Bme18I GGWCC 1 cut(s) 334
BmgT120I GGNCC 1 cut(s) 334
BmiI GGNNCC 3 cut(s) 279, 386, 474
BmsI GCATC 2 cut(s) 211, 665
BsaJI CCNNGG 1 cut(s) 337
BsaWI WCCGGW 1 cut(s) 37
BsaXI ACNNNNNCTCC 2 cut(s) 636, 666
Bsc4I CCNNNNNNNGG 1 cut(s) 419
Bse118I RCCGGY 1 cut(s) 613
Bse1I ACTGG 1 cut(s) 597
BseDI CCNNGG 1 cut(s) 337
BseGI GGATG 3 cut(s) 46, 156, 532
BseLI CCNNNNNNNGG 1 cut(s) 419
BseNI ACTGG 1 cut(s) 597
BseXI GCAGC 1 cut(s) 593
BseYI CCCAGC 1 cut(s) 448
BshFI GGCC 1 cut(s) 426
BsiSI CCGG 2 cut(s) 38, 614
BslI CCNNNNNNNGG 1 cut(s) 419
BsmAI GTCTC 2 cut(s) 452, 641
BsnI GGCC 1 cut(s) 426
Bsp1286I GDGCHC 1 cut(s) 389
Bsp143I GATC 3 cut(s) 472, 544, 589
Bsp19I CCATGG 1 cut(s) 337
BspACI CCGC 2 cut(s) 110, 160
BspANI GGCC 1 cut(s) 426
BspLI GGNNCC 3 cut(s) 279, 386, 474
BspPI GGATC 3 cut(s) 467, 480, 584
BspQI GCTCTTC 1 cut(s) 603
BspTI CTTAAG 1 cut(s) 17
BsrFI RCCGGY 1 cut(s) 613
BsrI ACTGG 1 cut(s) 597
BssAI RCCGGY 1 cut(s) 613
BssECI CCNNGG 1 cut(s) 337
BssMI GATC 3 cut(s) 472, 544, 589
BssSI CACGAG 1 cut(s) 585
BssT1I CCWWGG 1 cut(s) 337
Bst2BI CACGAG 1 cut(s) 585
Bst4CI ACNGT 3 cut(s) 350, 463, 652
Bst6I CTCTTC 2 cut(s) 243, 603
BstAFI CTTAAG 1 cut(s) 17
BstC8I GCNNGC 2 cut(s) 226, 615
BstDSI CCRYGG 1 cut(s) 337
BstF5I GGATG 3 cut(s) 46, 156, 532
BstKTI GATC 3 cut(s) 475, 547, 592
BstMAI GTCTC 2 cut(s) 452, 641
BstMBI GATC 3 cut(s) 472, 544, 589
BstV1I GCAGC 1 cut(s) 593
BstX2I RGATCY 1 cut(s) 472
BstYI RGATCY 1 cut(s) 472
BsuRI GGCC 1 cut(s) 426
BtgI CCRYGG 1 cut(s) 337
BtsCI GGATG 3 cut(s) 46, 156, 532
BtsIMutI CAGTG 1 cut(s) 355
Cac8I GCNNGC 2 cut(s) 226, 615
Cfr10I RCCGGY 1 cut(s) 613
Cfr13I GGNCC 1 cut(s) 334
CviAII CATG 5 cut(s) 296, 316, 338, 428, 445
CviJI RGCY 5 cut(s) 387, 426, 584, 613, 625
CviKI_1 RGCY 5 cut(s) 387, 426, 584, 613, 625
DpnI GATC 3 cut(s) 474, 546, 591
DpnII GATC 3 cut(s) 472, 544, 589
EaeI YGGCCR 1 cut(s) 424
Eam1104I CTCTTC 2 cut(s) 243, 603
EarI CTCTTC 2 cut(s) 243, 603
Eco130I CCWWGG 1 cut(s) 337
Eco24I GRGCYC 1 cut(s) 389
Eco47I GGWCC 1 cut(s) 334
Eco57I CTGAAG 2 cut(s) 120, 214
EcoT14I CCWWGG 1 cut(s) 337
EcoT38I GRGCYC 1 cut(s) 389
ErhI CCWWGG 1 cut(s) 337
FaeI CATG 5 cut(s) 299, 319, 341, 431, 448
FatI CATG 5 cut(s) 295, 315, 337, 427, 444
FauI CCCGC 1 cut(s) 103
Fnu4HI GCNGC 1 cut(s) 582
FokI GGATG 3 cut(s) 53, 143, 539
FriOI GRGCYC 1 cut(s) 389
Fsp4HI GCNGC 1 cut(s) 582
GluI GCNGC 1 cut(s) 582
GsaI CCCAGC 1 cut(s) 452
HaeIII GGCC 1 cut(s) 426
HapII CCGG 2 cut(s) 38, 614
Hin1II CATG 5 cut(s) 299, 319, 341, 431, 448
HinfI GANTC 3 cut(s) 235, 358, 500
HpaII CCGG 2 cut(s) 38, 614
HphI GGTGA 1 cut(s) 467
Hpy166II GTNNAC 2 cut(s) 122, 353
Hpy188I TCNGA 4 cut(s) 100, 505, 661, 668
Hpy188III TCNNGA 6 cut(s) 232, 242, 382, 524, 548, 587
Hpy8I GTNNAC 2 cut(s) 122, 353
HpyAV CCTTC 1 cut(s) 291
HpyCH4III ACNGT 3 cut(s) 350, 463, 652
HpyCH4IV ACGT 1 cut(s) 49
HpyCH4V TGCA 6 cut(s) 35, 431, 468, 515, 566, 656
HpySE526I ACGT 1 cut(s) 49
Hsp92II CATG 5 cut(s) 299, 319, 341, 431, 448
KroI GCCGGC 1 cut(s) 613
KroNI GCCGGC 1 cut(s) 615
Kzo9I GATC 3 cut(s) 472, 544, 589
LguI GCTCTTC 1 cut(s) 603
LmnI GCTCC 2 cut(s) 9, 384
LpnPI CCDG 9 cut(s) 51, 217, 367, 426, 434, 501, 509, 610, 627
Lsp1109I GCAGC 1 cut(s) 593
LweI GCATC 2 cut(s) 211, 665
MaeII ACGT 1 cut(s) 49
MaeIII GTNAC 1 cut(s) 344
MalI GATC 3 cut(s) 474, 546, 591
MboI GATC 3 cut(s) 472, 544, 589
MboII GAAGA 3 cut(s) 230, 617, 620
MflI RGATCY 1 cut(s) 472
MhlI GDGCHC 1 cut(s) 389
MlsI TGGCCA 1 cut(s) 426
MluCI AATT 3 cut(s) 94, 129, 480
MluNI TGGCCA 1 cut(s) 426
MlyI GAGTC 2 cut(s) 229, 367
MmeI TCCRAC 1 cut(s) 617
MnlI CCTC 9 cut(s) 36, 298, 325, 335, 370, 446, 486, 551, 625
Mox20I TGGCCA 1 cut(s) 426
MroNI GCCGGC 1 cut(s) 613
MscI TGGCCA 1 cut(s) 426
MseI TTAA 4 cut(s) 18, 105, 128, 402
Msp20I TGGCCA 1 cut(s) 426
MspCI CTTAAG 1 cut(s) 17
MspI CCGG 2 cut(s) 38, 614
NaeI GCCGGC 1 cut(s) 615
NcoI CCATGG 1 cut(s) 337
NdeII GATC 3 cut(s) 472, 544, 589
NgoMIV GCCGGC 1 cut(s) 613
NlaIII CATG 5 cut(s) 299, 319, 341, 431, 448
NlaIV GGNNCC 3 cut(s) 279, 386, 474
PciSI GCTCTTC 1 cut(s) 603
PdiI GCCGGC 1 cut(s) 615
PfeI GAWTC 1 cut(s) 500
PkrI GCNGC 1 cut(s) 583
PleI GAGTC 2 cut(s) 229, 366
PpsI GAGTC 2 cut(s) 229, 366
PspFI CCCAGC 1 cut(s) 448
PspN4I GGNNCC 3 cut(s) 279, 386, 474
PspPI GGNCC 1 cut(s) 334
PsuI RGATCY 1 cut(s) 472
SapI GCTCTTC 1 cut(s) 603
SaqAI TTAA 4 cut(s) 18, 105, 128, 402
SatI GCNGC 1 cut(s) 582
Sau3AI GATC 3 cut(s) 472, 544, 589
Sau96I GGNCC 1 cut(s) 334
SchI GAGTC 2 cut(s) 229, 367
SduI GDGCHC 1 cut(s) 389
SfaNI GCATC 2 cut(s) 211, 665
SinI GGWCC 1 cut(s) 334
SmlI CTYRAG 1 cut(s) 17
SmoI CTYRAG 1 cut(s) 17
Sse9I AATT 3 cut(s) 94, 129, 480
SsiI CCGC 2 cut(s) 110, 160
SspI AATATT 1 cut(s) 322
StyI CCWWGG 1 cut(s) 337
TaaI ACNGT 3 cut(s) 350, 463, 652
TaiI ACGT 1 cut(s) 52
TaqI TCGA 4 cut(s) 284, 406, 543, 549
TasI AATT 3 cut(s) 94, 129, 480
TfiI GAWTC 1 cut(s) 500
Tru1I TTAA 4 cut(s) 18, 105, 128, 402
Tru9I TTAA 4 cut(s) 18, 105, 128, 402
TscAI CASTG 1 cut(s) 355
TseI GCWGC 1 cut(s) 581
TspDTI ATGAA 2 cut(s) 60, 332
TspRI CASTG 1 cut(s) 355
Vha464I CTTAAG 1 cut(s) 17
VpaK11BI GGWCC 1 cut(s) 334
XapI RAATTY 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.