RchiOBHm_Chr5g0003501

DNA binding domain with preference for A/T rich regions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001372
Physical Location & Seq
Reverse (-)
2182555 .. 2186135
3581 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28481

Sequence Viewer

Length: 1020 bp
ATGAATCAGAATAAAGGAACAAGCTCTTTATCTTCACCAGATCTCCCTATGAAGCGGAAACGTGGACGTCCACGTAAGGATGAGAATTTAGTTCACGGAGAGAGCATGCCTGCAATGCCAGGACCTGATAGTTTGAAGAAACACAAACAGAGTGCGGGTACTAACGATGATGGTGAAGATGAAATGGTGGGTCGAGTGGTTTCTGGTGTTATTGAAGGTTCATTTGATGATGGCTATCTTCTGAATGTTAAGGTAGAAGACACCGACACTCAGCTGAGGGGTGTTGTATTTCTTCCGGGAAAGTTCACTCCAGTCACTGCAGCAAATGATGTTGCTCCTAATGTTAAGATGTGCAAAAGAAAAGAAGTTCCAATACCAGCTGTCAACCTGCAATCTCATCTTTATGGTTCTGCCCGCCCATCAGAGCAAAGTAACAGGCAACCTTCTGACGTGAAAAAGCAAATACCTGCAGTTCCAGACCAAATTCCACCCCGTATTCCTCAGTGTGGCATCCCAGAGTCTCTTAGAAGCCAACCAACCTCTTTTGTAATTCCTCCGGTTGATAATTTGCCAAAAGATGATGCCCCTACCTTAGGAGGTAAAGTCATACCACAGCAACAGTTGGAGCCTGGACTTGATAACCAATCCACCCATGTAATGACAAAGTCCGAGAATGATGAAGTTGTTGAACAGGAAAAAGTGTTGGAGGAAGTTGAACCGGCCACAACAATAACAGAGCCAACTGGTGTAGAGGACAATAAAGAATCAAAAACAGAAACATCATCTGAACCAATTGGTGAGTTACCAAGCATAGACACAGTACATAAGGATCCTCCAATTCAACCTCAGGCTGTGGGTTCTGAAAGCCGTGCATTGGTTCAGGACGACGTAAAGAGCCTGAATTTTGAAGTCAACCAAACTCCTGTATTTGCTGAAGCTACTCCTGCAATGGCTATCATACCAAGCGTTGAAACTGTATGCAAGGCTCCTCAAATTCAACCTCAAGACTGTGGATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

339

Amino Acids

36.67

Weight (kDa)

4.83

Isoelectric Point (pI)

60.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 70
Acc36I ACCTGC 2 cut(s) 396, 475
AciI CCGC 3 cut(s) 55, 155, 415
AclWI GGATC 2 cut(s) 824, 837
AcoI YGGCCR 1 cut(s) 720
AcsI RAATTY 4 cut(s) 85, 483, 901, 993
AcuI CTGAAG 1 cut(s) 954
AcyI GRCGYC 1 cut(s) 67
AfaI GTAC 2 cut(s) 160, 822
AfiI CCNNNNNNNGG 3 cut(s) 506, 593, 874
AgsI TTSAA 8 cut(s) 136, 215, 689, 716, 842, 908, 971, 998
AjiI CACGTC 1 cut(s) 451
AjnI CCWGG 2 cut(s) 118, 628
AluBI AGCT 4 cut(s) 24, 274, 380, 938
AluI AGCT 4 cut(s) 24, 274, 380, 938
Alw26I GTCTC 1 cut(s) 525
AlwI GGATC 2 cut(s) 824, 837
AlwNI CAGNNNCTG 2 cut(s) 125, 317
AoxI GGCC 1 cut(s) 720
ApeKI GCWGC 1 cut(s) 320
ApoI RAATTY 4 cut(s) 85, 483, 901, 993
AspS9I GGNCC 1 cut(s) 122
AsuC2I CCSGG 1 cut(s) 297
AsuHPI GGTGA 3 cut(s) 27, 185, 809
AvaII GGWCC 1 cut(s) 122
AxyI CCTNAGG 2 cut(s) 592, 846
BamHI GGATCC 1 cut(s) 829
BarI GAAGNNNNNNTAC 2 cut(s) 357, 389
BbsI GAAGAC 1 cut(s) 264
BbvCI CCTCAGC 1 cut(s) 275
BbvI GCAGC 1 cut(s) 332
BccI CCATC 3 cut(s) 164, 224, 427
BceAI ACGGC 1 cut(s) 852
BciT130I CCWGG 2 cut(s) 120, 630
BcnI CCSGG 1 cut(s) 297
BcoDI GTCTC 1 cut(s) 525
BfmI CTRYAG 2 cut(s) 318, 468
BfuAI ACCTGC 2 cut(s) 396, 475
BglII AGATCT 1 cut(s) 40
BisI GCNGC 1 cut(s) 321
BlsI GCNGC 1 cut(s) 322
Bme1390I CCNGG 3 cut(s) 120, 297, 630
Bme18I GGWCC 1 cut(s) 122
BmgBI CACGTC 1 cut(s) 451
BmgT120I GGNCC 1 cut(s) 122
BmiI GGNNCC 3 cut(s) 627, 831, 987
BmrFI CCNGG 3 cut(s) 120, 297, 630
BmsI GCATC 3 cut(s) 519, 571, 1004
BpiI GAAGAC 1 cut(s) 264
BpmI CTGGAG 1 cut(s) 294
Bpu10I CCTNAGC 1 cut(s) 275
BpuEI CTTGAG 1 cut(s) 987
BpuMI CCSGG 1 cut(s) 297
BsaAI YACGTR 1 cut(s) 74
BsaBI GATNNNNATC 1 cut(s) 234
BsaHI GRCGYC 1 cut(s) 67
BsaWI WCCGGW 1 cut(s) 556
BsaXI ACNNNNNCTCC 4 cut(s) 27, 57, 588, 618
Bsc4I CCNNNNNNNGG 3 cut(s) 506, 593, 874
Bse118I RCCGGY 1 cut(s) 718
Bse1I ACTGG 2 cut(s) 311, 748
Bse21I CCTNAGG 2 cut(s) 592, 846
Bse3DI GCAATG 2 cut(s) 120, 954
Bse8I GATNNNNATC 1 cut(s) 234
BseBI CCWGG 2 cut(s) 120, 630
BseGI GGATG 3 cut(s) 85, 510, 1019
BseJI GATNNNNATC 1 cut(s) 234
BseLI CCNNNNNNNGG 3 cut(s) 506, 593, 874
BseMI GCAATG 2 cut(s) 120, 954
BseMII CTCAG 4 cut(s) 266, 284, 515, 860
BseNI ACTGG 2 cut(s) 311, 748
BseRI GAGGAG 1 cut(s) 978
BseXI GCAGC 1 cut(s) 332
BshFI GGCC 1 cut(s) 722
BsiSI CCGG 3 cut(s) 296, 557, 719
BslI CCNNNNNNNGG 3 cut(s) 506, 593, 874
BsmAI GTCTC 1 cut(s) 525
BsnI GGCC 1 cut(s) 722
Bsp143I GATC 2 cut(s) 40, 829
BspACI CCGC 3 cut(s) 55, 155, 415
BspANI GGCC 1 cut(s) 722
BspCNI CTCAG 4 cut(s) 267, 283, 514, 859
BspLI GGNNCC 3 cut(s) 627, 831, 987
BspMAI CTGCAG 2 cut(s) 322, 472
BspMI ACCTGC 2 cut(s) 396, 475
BspPI GGATC 2 cut(s) 824, 837
BsrDI GCAATG 2 cut(s) 120, 954
BsrFI RCCGGY 1 cut(s) 718
BsrI ACTGG 2 cut(s) 311, 748
BssAI RCCGGY 1 cut(s) 718
BssMI GATC 2 cut(s) 40, 829
BssNI GRCGYC 1 cut(s) 67
Bst2UI CCWGG 2 cut(s) 120, 630
Bst4CI ACNGT 4 cut(s) 621, 820, 976, 1010
BstACI GRCGYC 1 cut(s) 67
BstBAI YACGTR 1 cut(s) 74
BstC8I GCNNGC 3 cut(s) 107, 111, 415
BstDEI CTNAG 6 cut(s) 270, 275, 501, 524, 592, 846
BstENI CCTNNNNNAGG 1 cut(s) 591
BstF5I GGATG 3 cut(s) 85, 510, 1019
BstKTI GATC 2 cut(s) 43, 832
BstMAI GTCTC 1 cut(s) 525
BstMBI GATC 2 cut(s) 40, 829
BstMWI GCNNNNNNNGC 2 cut(s) 115, 944
BstNI CCWGG 2 cut(s) 120, 630
BstNSI RCATGY 1 cut(s) 109
BstSCI CCNGG 3 cut(s) 118, 295, 628
BstSFI CTRYAG 2 cut(s) 318, 468
BstV1I GCAGC 1 cut(s) 332
BstV2I GAAGAC 1 cut(s) 264
BstX2I RGATCY 2 cut(s) 40, 829
BstYI RGATCY 2 cut(s) 40, 829
Bsu36I CCTNAGG 2 cut(s) 592, 846
BsuRI GGCC 1 cut(s) 722
BtrI CACGTC 1 cut(s) 451
BtsCI GGATG 3 cut(s) 85, 510, 1019
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 2 cut(s) 315, 509
BveI ACCTGC 2 cut(s) 396, 475
Cac8I GCNNGC 3 cut(s) 107, 111, 415
CaiI CAGNNNCTG 2 cut(s) 125, 317
Cfr10I RCCGGY 1 cut(s) 718
Cfr13I GGNCC 1 cut(s) 122
Csp6I GTAC 2 cut(s) 159, 821
CviAII CATG 2 cut(s) 106, 653
CviQI GTAC 2 cut(s) 159, 821
DdeI CTNAG 6 cut(s) 270, 275, 501, 524, 592, 846
DpnI GATC 2 cut(s) 42, 831
DpnII GATC 2 cut(s) 40, 829
EaeI YGGCCR 1 cut(s) 720
Eco47I GGWCC 1 cut(s) 122
Eco57I CTGAAG 1 cut(s) 954
Eco81I CCTNAGG 2 cut(s) 592, 846
EcoNI CCTNNNNNAGG 1 cut(s) 591
EcoO109I RGGNCCY 1 cut(s) 122
EcoRII CCWGG 2 cut(s) 118, 628
FaeI CATG 2 cut(s) 109, 656
FaiI YATR 9 cut(s) 50, 107, 405, 608, 654, 812, 825, 959, 979
FatI CATG 2 cut(s) 105, 652
FauI CCCGC 2 cut(s) 148, 422
Fnu4HI GCNGC 1 cut(s) 321
FokI GGATG 2 cut(s) 92, 497
Fsp4HI GCNGC 1 cut(s) 321
GluI GCNGC 1 cut(s) 321
GsuI CTGGAG 1 cut(s) 294
HaeIII GGCC 1 cut(s) 722
HapII CCGG 3 cut(s) 296, 557, 719
Hin1I GRCGYC 1 cut(s) 67
Hin1II CATG 2 cut(s) 109, 656
HincII GTYRAC 2 cut(s) 385, 913
HindII GTYRAC 2 cut(s) 385, 913
HinfI GANTC 3 cut(s) 4, 518, 764
HpaII CCGG 3 cut(s) 296, 557, 719
HphI GGTGA 3 cut(s) 27, 185, 809
Hpy166II GTNNAC 6 cut(s) 65, 71, 94, 306, 385, 913
Hpy188I TCNGA 7 cut(s) 9, 243, 424, 448, 670, 787, 862
Hpy188III TCNNGA 3 cut(s) 476, 881, 1004
Hpy8I GTNNAC 6 cut(s) 65, 71, 94, 306, 385, 913
Hpy99I CGWCG 1 cut(s) 890
HpyAV CCTTC 2 cut(s) 209, 453
HpyCH4III ACNGT 4 cut(s) 621, 820, 976, 1010
HpyCH4IV ACGT 5 cut(s) 61, 67, 73, 450, 888
HpyCH4V TGCA 8 cut(s) 113, 320, 354, 391, 470, 872, 947, 981
HpyF10VI GCNNNNNNNGC 2 cut(s) 115, 944
HpyF3I CTNAG 6 cut(s) 270, 275, 501, 524, 592, 846
HpySE526I ACGT 5 cut(s) 61, 67, 73, 450, 888
Hsp92I GRCGYC 1 cut(s) 67
Hsp92II CATG 2 cut(s) 109, 656
Kzo9I GATC 2 cut(s) 40, 829
LmnI GCTCC 3 cut(s) 340, 625, 991
Lsp1109I GCAGC 1 cut(s) 332
LweI GCATC 3 cut(s) 519, 571, 1004
MaeII ACGT 5 cut(s) 61, 67, 73, 450, 888
MaeIII GTNAC 3 cut(s) 313, 431, 801
MalI GATC 2 cut(s) 42, 831
MboI GATC 2 cut(s) 40, 829
MboII GAAGA 6 cut(s) 24, 148, 188, 230, 269, 284
MfeI CAATTG 1 cut(s) 792
MflI RGATCY 2 cut(s) 40, 829
MluCI AATT 8 cut(s) 85, 483, 549, 565, 792, 837, 901, 993
MlyI GAGTC 1 cut(s) 527
MmeI TCCRAC 2 cut(s) 603, 684
MseI TTAA 2 cut(s) 249, 345
MslI CAYNNNNRTG 1 cut(s) 402
MspA1I CMGCKG 2 cut(s) 274, 380
MspI CCGG 3 cut(s) 296, 557, 719
MspR9I CCNGG 3 cut(s) 120, 297, 630
MunI CAATTG 1 cut(s) 792
MvaI CCWGG 2 cut(s) 120, 630
MwoI GCNNNNNNNGC 2 cut(s) 115, 944
NciI CCSGG 1 cut(s) 297
NdeII GATC 2 cut(s) 40, 829
NlaIII CATG 2 cut(s) 109, 656
NlaIV GGNNCC 3 cut(s) 627, 831, 987
NmuCI GTSAC 1 cut(s) 313
NspI RCATGY 1 cut(s) 109
PaeI GCATGC 1 cut(s) 109
PfeI GAWTC 2 cut(s) 4, 764
PflFI GACNNNGTC 1 cut(s) 664
PfoI TCCNGGA 1 cut(s) 295
PkrI GCNGC 1 cut(s) 322
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
Ppu21I YACGTR 1 cut(s) 74
PpuMI RGGWCCY 1 cut(s) 122
Psp5II RGGWCCY 1 cut(s) 122
Psp6I CCWGG 2 cut(s) 118, 628
PspGI CCWGG 2 cut(s) 118, 628
PspN4I GGNNCC 3 cut(s) 627, 831, 987
PspPI GGNCC 1 cut(s) 122
PspPPI RGGWCCY 1 cut(s) 122
PstI CTGCAG 2 cut(s) 322, 472
PstNI CAGNNNCTG 2 cut(s) 125, 317
PsuI RGATCY 2 cut(s) 40, 829
PsyI GACNNNGTC 1 cut(s) 664
PvuII CAGCTG 2 cut(s) 274, 380
RsaI GTAC 2 cut(s) 160, 822
RsaNI GTAC 2 cut(s) 159, 821
RseI CAYNNNNRTG 1 cut(s) 402
SaqAI TTAA 2 cut(s) 249, 345
SatI GCNGC 1 cut(s) 321
Sau3AI GATC 2 cut(s) 40, 829
Sau96I GGNCC 1 cut(s) 122
SchI GAGTC 1 cut(s) 527
ScrFI CCNGG 3 cut(s) 120, 297, 630
SfaNI GCATC 3 cut(s) 519, 571, 1004
SfcI CTRYAG 2 cut(s) 318, 468
SinI GGWCC 1 cut(s) 122
SmiMI CAYNNNNRTG 1 cut(s) 402
SmlI CTYRAG 1 cut(s) 1002
SmoI CTYRAG 1 cut(s) 1002
SphI GCATGC 1 cut(s) 109
Sse9I AATT 8 cut(s) 85, 483, 549, 565, 792, 837, 901, 993
SsiI CCGC 3 cut(s) 55, 155, 415
StyD4I CCNGG 3 cut(s) 118, 295, 628
TaaI ACNGT 4 cut(s) 621, 820, 976, 1010
TaiI ACGT 5 cut(s) 64, 70, 76, 453, 891
TaqI TCGA 1 cut(s) 193
TasI AATT 8 cut(s) 85, 483, 549, 565, 792, 837, 901, 993
TatI WGTACW 1 cut(s) 820
TfiI GAWTC 2 cut(s) 4, 764
Tru1I TTAA 2 cut(s) 249, 345
Tru9I TTAA 2 cut(s) 249, 345
TscAI CASTG 2 cut(s) 322, 509
TseFI GTSAC 1 cut(s) 313
TseI GCWGC 1 cut(s) 320
Tsp45I GTSAC 1 cut(s) 313
TspDTI ATGAA 5 cut(s) 17, 65, 195, 210, 693
TspGWI ACGGA 1 cut(s) 111
TspRI CASTG 2 cut(s) 322, 509
Tth111I GACNNNGTC 1 cut(s) 664
VpaK11BI GGWCC 1 cut(s) 122
XagI CCTNNNNNAGG 1 cut(s) 591
XapI RAATTY 4 cut(s) 85, 483, 901, 993
XceI RCATGY 1 cut(s) 109
ZraI GACGTC 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.