RchiOBHm_Chr5g0003491

DNA binding domain with preference for A/T rich regions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001372
Physical Location & Seq
Forward (+)
2177359 .. 2181491
4133 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28480

Sequence Viewer

Length: 1509 bp
ATGAATCAGAGTCAAGGAACAAGCTCTTTATCTTCGCCAGATCTCCCTATGAAGCGGAAACGTGGACGTCCACGAAAGGATGAGAATTTAGTTCACGGAGAGAGCACGCCTGCAATGCCAGGACCTGATAGTTTGAAGAAAAACAAACAGAGTGCGGGTACTAACGATGATGGTGAAGATGAAATGGTGGGTCGAGTGGTTTCTGGTGTTATTGAAGGTTCATTTGATGCCGGCTATCTTCTGAATGTTAAGGTAGAAGACACCGACACTCAGCTGAGGGGTGTTGTATTTCTTCCGGGAAAGTTCACTCCAGTCACTGCAGCAAATGATGTTGCTCCTAATGTTAAGATGTGCAAAAGAAAAGAAGTTCCAATACCAGCTGTCAACCTGCAATCTCATCTTTATGGTTCTGCCCGGCCATCAGAGCAAAGTAACAGGCCACCTGTTGAGGTGAAAAAGCAAATACCTGCAATTCCAGACCAAATTCCTCCCCGTATTCCTCAGTGTGGCATCCCGGAGACTCTTAGAAGTCAATCTTTCTCTGGCATCCCAGAGTCTCTTCAAAGCCAACGCATCCAGGAGTCTCTTAGAAGTCAATCTTTCCCTTGCATCCCGGAGTCCTCTTTTGTAAATCTTCCGGTTGATAATTTGCCAAAGAATGATGCCCCTGTCTTGGGAGGTAAAGTCACACCACAGCAACCTTTGGATCCTGGACTTGATAACCAATCCACCCATTCTGAGAATGATGAAGTTGTTGAACAGGAAAAAGTGTTGGAGGAAGTTGAACCGGCCACAACAATAACAGAGCCAACTGGTGTAGAGGACAATAAAGAATCAAAGACAGAAACATCATCTGAACCAATTGGTGAGGTACTAAGCATAGACACAGTATGTAAGGATCCTCCAATTCAACCTCAGGCTGTGGGTTCTGAAAGCAGTGCATTGGTTCAGGACGACGTAAAGAGCATGAATTTTGAACTCAACCATATTCCTCTATTTGCTGAAGCTGCTCCTGCAATGGCTACCATACAAAGCGTTGAAACTGTATGCAAGGCTCCTCAAATTCAACCTCAGACTGTGGATACTGAAAGGAGTCAATTGGTTGATGATGAGGTAAAAAGTGTGAACGTTGAACTCAATCAAACTCCTGTATTTGCTGAACCCGCAACCAGTTCTGTCATACTGGGAGTTGAGACTGTATGTAAGGATCCTCAAATTCAACCTGAGGATGTGGGTTCTGAAAAGAGTGCATTGGTTCAGGATGTGGTAAAGAGCCTACATCTTGAGCTCAACCAAACTCTTCTGGTTGCAGAACCTGCATTTGCAGCCATTAAACCAGTTGACAATCTGATGGAAGAAGAGGCTGCACCAAAGCCGAGTCAGGTGTTAGGGGGAGGGACTGTTGCATCTGTAGGATCCATTCCTTCAGCCATGGGTGAACCTCCTTCTGGTGCTGTTGACAGTATGGAATGTGATATTGCAGATGCCATTTCACCTGCACAATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

502

Amino Acids

53.57

Weight (kDa)

4.49

Isoelectric Point (pI)

62.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1504
AatII GACGTC 1 cut(s) 70
Acc36I ACCTGC 4 cut(s) 396, 475, 1324, 1504
AciI CCGC 3 cut(s) 55, 155, 1164
AclI AACGTT 1 cut(s) 1128
AclWI GGATC 8 cut(s) 701, 714, 893, 906, 1202, 1215, 1410, 1423
AcoI YGGCCR 2 cut(s) 416, 789
AcsI RAATTY 5 cut(s) 85, 483, 970, 1062, 1215
AcuI CTGAAG 2 cut(s) 1023, 1410
AcyI GRCGYC 1 cut(s) 67
AfaI GTAC 2 cut(s) 160, 873
AfiI CCNNNNNNNGG 4 cut(s) 506, 673, 674, 1448
AjnI CCWGG 3 cut(s) 118, 576, 709
AluBI AGCT 5 cut(s) 24, 274, 380, 1007, 1288
AluI AGCT 5 cut(s) 24, 274, 380, 1007, 1288
Alw21I GWGCWC 2 cut(s) 107, 1290
Alw26I GTCTC 4 cut(s) 512, 561, 588, 1187
AlwI GGATC 8 cut(s) 701, 714, 893, 906, 1202, 1215, 1410, 1423
AlwNI CAGNNNCTG 3 cut(s) 125, 317, 1316
AoxI GGCC 3 cut(s) 416, 437, 789
ApeKI GCWGC 4 cut(s) 320, 1007, 1325, 1364
ApoI RAATTY 5 cut(s) 85, 483, 970, 1062, 1215
AspS9I GGNCC 1 cut(s) 122
AsuC2I CCSGG 4 cut(s) 297, 415, 515, 614
AsuHPI GGTGA 5 cut(s) 185, 463, 878, 1448, 1485
AvaII GGWCC 1 cut(s) 122
AxyI CCTNAGG 2 cut(s) 915, 1224
BamHI GGATCC 4 cut(s) 706, 898, 1207, 1415
BanII GRGCYC 1 cut(s) 1290
BarI GAAGNNNNNNTAC 2 cut(s) 357, 389
BbsI GAAGAC 1 cut(s) 264
Bbv12I GWGCWC 2 cut(s) 107, 1290
BbvCI CCTCAGC 1 cut(s) 275
BbvI GCAGC 4 cut(s) 332, 994, 1337, 1351
BccI CCATC 3 cut(s) 164, 427, 1345
BciT130I CCWGG 3 cut(s) 120, 578, 711
BciVI GTATCC 1 cut(s) 1075
BcnI CCSGG 4 cut(s) 297, 415, 515, 614
BcoDI GTCTC 4 cut(s) 512, 561, 588, 1187
BfmI CTRYAG 2 cut(s) 318, 1410
BfuAI ACCTGC 4 cut(s) 396, 475, 1324, 1504
BfuI GTATCC 1 cut(s) 1075
BglII AGATCT 1 cut(s) 40
BisI GCNGC 4 cut(s) 321, 1008, 1326, 1365
BlsI GCNGC 4 cut(s) 322, 1009, 1327, 1366
Bme1390I CCNGG 7 cut(s) 120, 297, 415, 515, 578, 614, 711
Bme18I GGWCC 1 cut(s) 122
BmgT120I GGNCC 1 cut(s) 122
BmiI GGNNCC 5 cut(s) 708, 900, 1056, 1209, 1417
BmrFI CCNGG 7 cut(s) 120, 297, 415, 515, 578, 614, 711
BmrI ACTGGG 1 cut(s) 1193
BmsI GCATC 8 cut(s) 217, 519, 555, 582, 618, 652, 1415, 1474
BmuI ACTGGG 1 cut(s) 1193
BpiI GAAGAC 1 cut(s) 264
BpmI CTGGAG 1 cut(s) 294
Bpu10I CCTNAGC 1 cut(s) 275
BpuEI CTTGAG 1 cut(s) 1304
BpuMI CCSGG 4 cut(s) 297, 415, 515, 614
BsaHI GRCGYC 1 cut(s) 67
BsaJI CCNNGG 1 cut(s) 1431
BsaWI WCCGGW 1 cut(s) 637
BsaXI ACNNNNNCTCC 4 cut(s) 669, 699, 1386, 1416
Bsc4I CCNNNNNNNGG 4 cut(s) 506, 673, 674, 1448
Bse118I RCCGGY 2 cut(s) 230, 787
Bse1I ACTGG 5 cut(s) 311, 817, 1170, 1188, 1337
Bse21I CCTNAGG 2 cut(s) 915, 1224
Bse3DI GCAATG 2 cut(s) 120, 1023
BseBI CCWGG 3 cut(s) 120, 578, 711
BseDI CCNNGG 1 cut(s) 1431
BseGI GGATG 7 cut(s) 85, 510, 546, 573, 609, 1234, 1267
BseLI CCNNNNNNNGG 4 cut(s) 506, 673, 674, 1448
BseMI GCAATG 2 cut(s) 120, 1023
BseMII CTCAG 7 cut(s) 266, 284, 515, 729, 929, 1085, 1215
BseNI ACTGG 5 cut(s) 311, 817, 1170, 1188, 1337
BseRI GAGGAG 1 cut(s) 1047
BseXI GCAGC 4 cut(s) 332, 994, 1337, 1351
BsgI GTGCAG 2 cut(s) 1350, 1482
BshFI GGCC 3 cut(s) 418, 439, 791
BsiHKAI GWGCWC 2 cut(s) 107, 1290
BsiSI CCGG 7 cut(s) 231, 296, 415, 515, 614, 638, 788
BslFI GGGAC 1 cut(s) 1411
BslI CCNNNNNNNGG 4 cut(s) 506, 673, 674, 1448
BsmAI GTCTC 4 cut(s) 512, 561, 588, 1187
BsmFI GGGAC 1 cut(s) 1411
BsnI GGCC 3 cut(s) 418, 439, 791
Bsp1286I GDGCHC 2 cut(s) 107, 1290
Bsp143I GATC 5 cut(s) 40, 706, 898, 1207, 1415
Bsp19I CCATGG 1 cut(s) 1431
BspACI CCGC 3 cut(s) 55, 155, 1164
BspANI GGCC 3 cut(s) 418, 439, 791
BspCNI CTCAG 7 cut(s) 267, 283, 514, 730, 928, 1084, 1216
BspLI GGNNCC 5 cut(s) 708, 900, 1056, 1209, 1417
BspMAI CTGCAG 1 cut(s) 322
BspMI ACCTGC 4 cut(s) 396, 475, 1324, 1504
BspPI GGATC 8 cut(s) 701, 714, 893, 906, 1202, 1215, 1410, 1423
BsrDI GCAATG 2 cut(s) 120, 1023
BsrFI RCCGGY 2 cut(s) 230, 787
BsrI ACTGG 5 cut(s) 311, 817, 1170, 1188, 1337
BssAI RCCGGY 2 cut(s) 230, 787
BssECI CCNNGG 1 cut(s) 1431
BssMI GATC 5 cut(s) 40, 706, 898, 1207, 1415
BssNI GRCGYC 1 cut(s) 67
BssT1I CCWWGG 1 cut(s) 1431
Bst2UI CCWGG 3 cut(s) 120, 578, 711
Bst4CI ACNGT 6 cut(s) 889, 1045, 1078, 1198, 1402, 1463
Bst6I CTCTTC 3 cut(s) 564, 1305, 1353
BstACI GRCGYC 1 cut(s) 67
BstAPI GCANNNNNTGC 1 cut(s) 1316
BstC8I GCNNGC 3 cut(s) 107, 111, 232
BstDSI CCRYGG 1 cut(s) 1431
BstF5I GGATG 7 cut(s) 85, 510, 546, 573, 609, 1234, 1267
BstKTI GATC 5 cut(s) 43, 709, 901, 1210, 1418
BstMAI GTCTC 4 cut(s) 512, 561, 588, 1187
BstMBI GATC 5 cut(s) 40, 706, 898, 1207, 1415
BstMWI GCNNNNNNNGC 7 cut(s) 115, 424, 1007, 1013, 1163, 1316, 1325
BstNI CCWGG 3 cut(s) 120, 578, 711
BstSCI CCNGG 7 cut(s) 118, 295, 413, 513, 576, 612, 709
BstSFI CTRYAG 2 cut(s) 318, 1410
BstV1I GCAGC 4 cut(s) 332, 994, 1337, 1351
BstV2I GAAGAC 1 cut(s) 264
BstX2I RGATCY 5 cut(s) 40, 706, 898, 1207, 1415
BstYI RGATCY 5 cut(s) 40, 706, 898, 1207, 1415
Bsu36I CCTNAGG 2 cut(s) 915, 1224
BsuI GTATCC 1 cut(s) 1075
BsuRI GGCC 3 cut(s) 418, 439, 791
BtgI CCRYGG 1 cut(s) 1431
BtsCI GGATG 7 cut(s) 85, 510, 546, 573, 609, 1234, 1267
BtsI GCAGTG 2 cut(s) 315, 943
BtsIMutI CAGTG 3 cut(s) 315, 509, 943
BveI ACCTGC 4 cut(s) 396, 475, 1324, 1504
Cac8I GCNNGC 3 cut(s) 107, 111, 232
CaiI CAGNNNCTG 3 cut(s) 125, 317, 1316
Cfr10I RCCGGY 2 cut(s) 230, 787
Cfr13I GGNCC 1 cut(s) 122
Csp6I GTAC 2 cut(s) 159, 872
CviAII CATG 2 cut(s) 967, 1432
CviQI GTAC 2 cut(s) 159, 872
DpnI GATC 5 cut(s) 42, 708, 900, 1209, 1417
DpnII GATC 5 cut(s) 40, 706, 898, 1207, 1415
EaeI YGGCCR 2 cut(s) 416, 789
Eam1104I CTCTTC 3 cut(s) 564, 1305, 1353
EarI CTCTTC 3 cut(s) 564, 1305, 1353
Ecl136II GAGCTC 1 cut(s) 1288
Eco130I CCWWGG 1 cut(s) 1431
Eco24I GRGCYC 1 cut(s) 1290
Eco47I GGWCC 1 cut(s) 122
Eco53kI GAGCTC 1 cut(s) 1288
Eco57I CTGAAG 2 cut(s) 1023, 1410
Eco81I CCTNAGG 2 cut(s) 915, 1224
EcoICRI GAGCTC 1 cut(s) 1288
EcoO109I RGGNCCY 1 cut(s) 122
EcoRII CCWGG 3 cut(s) 118, 576, 709
EcoT14I CCWWGG 1 cut(s) 1431
EcoT38I GRGCYC 1 cut(s) 1290
ErhI CCWWGG 1 cut(s) 1431
FaeI CATG 2 cut(s) 970, 1435
FalI AAGNNNNNCTT 4 cut(s) 520, 552, 583, 615
FaqI GGGAC 1 cut(s) 1411
FatI CATG 2 cut(s) 966, 1431
FauI CCCGC 2 cut(s) 148, 1171
Fnu4HI GCNGC 4 cut(s) 321, 1008, 1326, 1365
FokI GGATG 7 cut(s) 92, 497, 533, 560, 596, 1241, 1274
FriOI GRGCYC 1 cut(s) 1290
Fsp4HI GCNGC 4 cut(s) 321, 1008, 1326, 1365
GluI GCNGC 4 cut(s) 321, 1008, 1326, 1365
GsuI CTGGAG 1 cut(s) 294
HaeIII GGCC 3 cut(s) 418, 439, 791
HapII CCGG 7 cut(s) 231, 296, 415, 515, 614, 638, 788
Hin1I GRCGYC 1 cut(s) 67
Hin1II CATG 2 cut(s) 970, 1435
HincII GTYRAC 3 cut(s) 385, 1342, 1459
HindII GTYRAC 3 cut(s) 385, 1342, 1459
HinfI GANTC 9 cut(s) 4, 10, 520, 554, 581, 617, 833, 1093, 1378
HpaII CCGG 7 cut(s) 231, 296, 415, 515, 614, 638, 788
HphI GGTGA 5 cut(s) 185, 463, 878, 1448, 1485
Hpy166II GTNNAC 9 cut(s) 65, 71, 94, 306, 385, 1126, 1342, 1439, 1459
Hpy188I TCNGA 9 cut(s) 9, 243, 424, 739, 856, 931, 1074, 1240, 1350
Hpy188III TCNNGA 4 cut(s) 476, 950, 1259, 1283
Hpy8I GTNNAC 9 cut(s) 65, 71, 94, 306, 385, 1126, 1342, 1439, 1459
Hpy99I CGWCG 1 cut(s) 959
HpyAV CCTTC 3 cut(s) 209, 1434, 1455
HpyCH4III ACNGT 6 cut(s) 889, 1045, 1078, 1198, 1402, 1463
HpyCH4IV ACGT 4 cut(s) 61, 67, 957, 1128
HpyF10VI GCNNNNNNNGC 7 cut(s) 115, 424, 1007, 1013, 1163, 1316, 1325
HpySE526I ACGT 4 cut(s) 61, 67, 957, 1128
Hsp92I GRCGYC 1 cut(s) 67
Hsp92II CATG 2 cut(s) 970, 1435
KroI GCCGGC 1 cut(s) 230
KroNI GCCGGC 1 cut(s) 232
Kzo9I GATC 5 cut(s) 40, 706, 898, 1207, 1415
LmnI GCTCC 3 cut(s) 340, 1015, 1060
Lsp1109I GCAGC 4 cut(s) 332, 994, 1337, 1351
LweI GCATC 8 cut(s) 217, 519, 555, 582, 618, 652, 1415, 1474
MaeII ACGT 4 cut(s) 61, 67, 957, 1128
MaeIII GTNAC 3 cut(s) 313, 431, 685
MalI GATC 5 cut(s) 42, 708, 900, 1209, 1417
MboI GATC 5 cut(s) 40, 706, 898, 1207, 1415
MfeI CAATTG 2 cut(s) 861, 1097
MflI RGATCY 5 cut(s) 40, 706, 898, 1207, 1415
MhlI GDGCHC 2 cut(s) 107, 1290
MlyI GAGTC 7 cut(s) 19, 514, 563, 590, 626, 1102, 1387
MmeI TCCRAC 1 cut(s) 753
MroNI GCCGGC 1 cut(s) 230
MseI TTAA 3 cut(s) 249, 345, 1332
MslI CAYNNNNRTG 1 cut(s) 402
MspA1I CMGCKG 2 cut(s) 274, 380
MspI CCGG 7 cut(s) 231, 296, 415, 515, 614, 638, 788
MspR9I CCNGG 7 cut(s) 120, 297, 415, 515, 578, 614, 711
MunI CAATTG 2 cut(s) 861, 1097
MvaI CCWGG 3 cut(s) 120, 578, 711
MwoI GCNNNNNNNGC 7 cut(s) 115, 424, 1007, 1013, 1163, 1316, 1325
NaeI GCCGGC 1 cut(s) 232
NciI CCSGG 4 cut(s) 297, 415, 515, 614
NcoI CCATGG 1 cut(s) 1431
NdeII GATC 5 cut(s) 40, 706, 898, 1207, 1415
NgoMIV GCCGGC 1 cut(s) 230
NlaIII CATG 2 cut(s) 970, 1435
NlaIV GGNNCC 5 cut(s) 708, 900, 1056, 1209, 1417
NmeAIII GCCGAG 1 cut(s) 1401
NmuCI GTSAC 2 cut(s) 313, 685
PaqCI CACCTGC 1 cut(s) 1504
PdiI GCCGGC 1 cut(s) 232
PfeI GAWTC 2 cut(s) 4, 833
PfoI TCCNGGA 5 cut(s) 295, 513, 576, 612, 709
PkrI GCNGC 4 cut(s) 322, 1009, 1327, 1366
PleI GAGTC 7 cut(s) 18, 514, 562, 589, 625, 1101, 1386
PpsI GAGTC 7 cut(s) 18, 514, 562, 589, 625, 1101, 1386
PpuMI RGGWCCY 1 cut(s) 122
Psp124BI GAGCTC 1 cut(s) 1290
Psp1406I AACGTT 1 cut(s) 1128
Psp5II RGGWCCY 1 cut(s) 122
Psp6I CCWGG 3 cut(s) 118, 576, 709
PspGI CCWGG 3 cut(s) 118, 576, 709
PspN4I GGNNCC 5 cut(s) 708, 900, 1056, 1209, 1417
PspPI GGNCC 1 cut(s) 122
PspPPI RGGWCCY 1 cut(s) 122
PstI CTGCAG 1 cut(s) 322
PstNI CAGNNNCTG 3 cut(s) 125, 317, 1316
PsuI RGATCY 5 cut(s) 40, 706, 898, 1207, 1415
PvuII CAGCTG 2 cut(s) 274, 380
RsaI GTAC 2 cut(s) 160, 873
RsaNI GTAC 2 cut(s) 159, 872
RseI CAYNNNNRTG 1 cut(s) 402
SacI GAGCTC 1 cut(s) 1290
SaqAI TTAA 3 cut(s) 249, 345, 1332
SatI GCNGC 4 cut(s) 321, 1008, 1326, 1365
Sau3AI GATC 5 cut(s) 40, 706, 898, 1207, 1415
Sau96I GGNCC 1 cut(s) 122
SchI GAGTC 7 cut(s) 19, 514, 563, 590, 626, 1102, 1387
ScrFI CCNGG 7 cut(s) 120, 297, 415, 515, 578, 614, 711
SduI GDGCHC 2 cut(s) 107, 1290
SfaNI GCATC 8 cut(s) 217, 519, 555, 582, 618, 652, 1415, 1474
SfcI CTRYAG 2 cut(s) 318, 1410
SinI GGWCC 1 cut(s) 122
SmiMI CAYNNNNRTG 1 cut(s) 402
SmlI CTYRAG 1 cut(s) 1283
SmoI CTYRAG 1 cut(s) 1283
SsiI CCGC 3 cut(s) 55, 155, 1164
SstI GAGCTC 1 cut(s) 1290
StyD4I CCNGG 7 cut(s) 118, 295, 413, 513, 576, 612, 709
StyI CCWWGG 1 cut(s) 1431
TaaI ACNGT 6 cut(s) 889, 1045, 1078, 1198, 1402, 1463
TaiI ACGT 4 cut(s) 64, 70, 960, 1131
TaqI TCGA 1 cut(s) 193
TfiI GAWTC 2 cut(s) 4, 833
Tru1I TTAA 3 cut(s) 249, 345, 1332
Tru9I TTAA 3 cut(s) 249, 345, 1332
TscAI CASTG 3 cut(s) 322, 509, 943
TseFI GTSAC 2 cut(s) 313, 685
TseI GCWGC 4 cut(s) 320, 1007, 1325, 1364
Tsp45I GTSAC 2 cut(s) 313, 685
TspDTI ATGAA 6 cut(s) 17, 65, 195, 210, 762, 983
TspGWI ACGGA 1 cut(s) 111
TspRI CASTG 3 cut(s) 322, 509, 943
VpaK11BI GGWCC 1 cut(s) 122
XapI RAATTY 5 cut(s) 85, 483, 970, 1062, 1215
ZraI GACGTC 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.