MD10G1319800.v1.1

DNA binding domain with preference for A/T rich regions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001372
Physical Location & Seq
Reverse (-)
40181915 .. 40185527
3613 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1319800.v1.1.491

Sequence Viewer

Length: 1449 bp
ATGAATCAACAGATTCAGGGAACAGGCTCTCTATCTTCGTCAGACCCCCCTATGAAGCGAAAACGTGGACGTCCACGTAAGGATGAAAACTTGGCTCCTGCAGAGAACCCAACTGTGATCCCAGGACCTGACAGTTCGAAGAAAACCAAACAGAATGCAAGTACAAGTGATGATGTTGAGAATGAAATGGTAGGCCAGGTAGTCACTGGCGTGATTGAGGGGTCATTTGATGCTGGATATCTTCTGAATGTTAGGGTCGGAGACACCGACACTCAGCTGAGGGGTGTTGTGTTTCTGCAGGAGAAAATCACTCCCATTACTCCAGCAAATGACATTGCTCCTCATATTATGATGTGCAAAAGAAGAGAAATTCCCATACCGCCTCTCAACCCACAATCTCAGCGTTATGAGTCCGCCCACCCATCAGAACAAAGTAATAGGCAACCTGTTGAGGTGAAACCCCAAATACCTACTGTTCCTAACCAAATTCAATCCTCTGTCCCTCAGTCTGGAATCCTGGAGTCACTTGAAAACAAACCTTTCTCTTTTGTGACTCCGCCGGTTGTGAACTTGCCGAAGAATGATGCAGTCCCGCCCTTGGGAGGAAAAGTCATGCCACAGCAATTTCCGGAACCTGGAACTGATAACCAATCTGCCCCTACTATGCCCAAGCCAGAGCATAATAACGTTGTTGAACAGGAGAAAGTGCTGGAGGAAGTTGAAGCTGCCACAATAATGGAAGGGCCAAATGGTGTAGAGGCCAATGAGGCATCAAAAACAGAAACAGCAGCTGGACCAATTGCTGCCCTACCAGGTGTTGAACCCGTATGTAAGGCTCCTGAAATTCAAATTCAGGCTGCGGGTTCTGAAAAGAGTGCATCGGCTCCTTATGAAGTAAAGAGATTCAGACTTGAACTCAACCAAATGCCTGTATTCGGTGAACCTAGAGTGACCACTGTCATGCCAGTTGTTGAACTCAACCACACGCCTGTATTTGCTGAATCCGCAAGAATTGCTGCCATGCCAGTCATCGAAACCAAAGGTAAGGATAATCAAATTCAACCTCAGGCTGTGGATTCTGAAAAGAGCGCATTGGTTCATGATGGTGCAAAAAGCCCGAATCTCGAGCTCAACCAAACTCCTGTATTTGCTGAACCTGCCTCTGTTGCTCTCATACCAGGCATTGAAACCGTATGCAAGAATTCTCAAATTCAACCTCCAGATGTCAGTCCTGAAACGAGTTCATTGGTTCATGATGCGGTAAAGTGCGTGGACATAGAACTCAACCAAACTCCTTTTGCTGCTCCGGTTACTATACCATCCGAATCAAAGATTGCTGCAGAAGGATCTATTCCTCTGGGAACTGCCGAACCTCAAATCCCTACTTCTGATGGCATTAGCAACATGGAGTATGACGTCGAAGATGTCATTCCAGCTGCACAATCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

483

Amino Acids

51.58

Weight (kDa)

4.73

Isoelectric Point (pI)

56.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 73, 1419
Acc36I ACCTGC 1 cut(s) 1165
AccIII TCCGGA 1 cut(s) 628
AciI CCGC 7 cut(s) 380, 414, 557, 593, 860, 1005, 1259
AclI AACGTT 1 cut(s) 687
AclWI GGATC 2 cut(s) 112, 1354
AcsI RAATTY 7 cut(s) 369, 486, 843, 849, 1056, 1201, 1209
AcyI GRCGYC 2 cut(s) 70, 1416
AfaI GTAC 1 cut(s) 163
AfiI CCNNNNNNNGG 6 cut(s) 509, 598, 599, 602, 635, 935
AjnI CCWGG 6 cut(s) 121, 195, 516, 634, 811, 1177
AleI CACNNNNGTG 1 cut(s) 209
AloI GAACNNNNNNTCC 2 cut(s) 1362, 1394
AluBI AGCT 5 cut(s) 277, 725, 791, 1129, 1436
AluI AGCT 5 cut(s) 277, 725, 791, 1129, 1436
Alw21I GWGCWC 1 cut(s) 1131
Alw26I GTCTC 1 cut(s) 255
AlwI GGATC 2 cut(s) 112, 1354
AlwNI CAGNNNCTG 2 cut(s) 128, 791
Ama87I CYCGRG 1 cut(s) 1124
Aor13HI TCCGGA 1 cut(s) 628
AoxI GGCC 3 cut(s) 193, 743, 759
ApeKI GCWGC 8 cut(s) 725, 788, 803, 857, 1016, 1301, 1337, 1436
ApoI RAATTY 7 cut(s) 369, 486, 843, 849, 1056, 1201, 1209
AspLEI GCGC 1 cut(s) 1091
AspS9I GGNCC 3 cut(s) 125, 743, 794
AsuHPI GGTGA 2 cut(s) 466, 950
AsuII TTCGAA 1 cut(s) 137
AvaI CYCGRG 1 cut(s) 1124
AvaII GGWCC 2 cut(s) 125, 794
AxyI CCTNAGG 1 cut(s) 1065
BanII GRGCYC 1 cut(s) 1131
Bbv12I GWGCWC 1 cut(s) 1131
BbvCI CCTCAGC 1 cut(s) 278
BbvI GCAGC 8 cut(s) 712, 790, 800, 844, 1003, 1288, 1324, 1423
BccI CCATC 4 cut(s) 430, 1097, 1327, 1385
BciT130I CCWGG 6 cut(s) 123, 197, 518, 636, 813, 1179
BcoDI GTCTC 1 cut(s) 255
BfaI CTAG 1 cut(s) 945
BfmI CTRYAG 3 cut(s) 99, 296, 1338
BfuAI ACCTGC 1 cut(s) 1165
BglI GCCNNNNNGGC 1 cut(s) 767
BisI GCNGC 8 cut(s) 726, 789, 804, 858, 1017, 1302, 1338, 1437
BlsI GCNGC 8 cut(s) 727, 790, 805, 859, 1018, 1303, 1339, 1438
Bme1390I CCNGG 6 cut(s) 123, 197, 518, 636, 813, 1179
Bme18I GGWCC 2 cut(s) 125, 794
BmeT110I CYCGRG 1 cut(s) 1124
BmgT120I GGNCC 3 cut(s) 125, 743, 794
BmiI GGNNCC 4 cut(s) 96, 633, 837, 885
BmrFI CCNGG 6 cut(s) 123, 197, 518, 636, 813, 1179
BmsI GCATC 5 cut(s) 220, 574, 779, 887, 1246
BoxI GACNNNNGTC 1 cut(s) 956
BpmI CTGGAG 4 cut(s) 306, 539, 731, 1203
Bpu10I CCTNAGC 1 cut(s) 278
Bpu14I TTCGAA 1 cut(s) 137
BsaAI YACGTR 1 cut(s) 77
BsaHI GRCGYC 2 cut(s) 70, 1416
BsaJI CCNNGG 2 cut(s) 121, 597
BsaWI WCCGGW 2 cut(s) 628, 1306
BsaXI ACNNNNNCTCC 2 cut(s) 594, 624
Bsc4I CCNNNNNNNGG 6 cut(s) 509, 598, 599, 602, 635, 935
Bse118I RCCGGY 1 cut(s) 559
Bse1I ACTGG 3 cut(s) 211, 965, 1025
Bse21I CCTNAGG 1 cut(s) 1065
Bse3DI GCAATG 1 cut(s) 333
BseAI TCCGGA 1 cut(s) 628
BseBI CCWGG 6 cut(s) 123, 197, 518, 636, 813, 1179
BseDI CCNNGG 2 cut(s) 121, 597
BseGI GGATG 2 cut(s) 88, 1319
BseLI CCNNNNNNNGG 6 cut(s) 509, 598, 599, 602, 635, 935
BseMI GCAATG 1 cut(s) 333
BseMII CTCAG 5 cut(s) 269, 287, 413, 518, 1079
BseNI ACTGG 3 cut(s) 211, 965, 1025
BseRI GAGGAG 1 cut(s) 330
BseXI GCAGC 8 cut(s) 712, 790, 800, 844, 1003, 1288, 1324, 1423
BsgI GTGCAG 1 cut(s) 1422
BshFI GGCC 3 cut(s) 195, 745, 761
BsiHKAI GWGCWC 1 cut(s) 1131
BsiHKCI CYCGRG 1 cut(s) 1124
BsiSI CCGG 3 cut(s) 560, 629, 1307
BslFI GGGAC 2 cut(s) 485, 575
BslI CCNNNNNNNGG 6 cut(s) 509, 598, 599, 602, 635, 935
BsmAI GTCTC 1 cut(s) 255
BsmFI GGGAC 2 cut(s) 485, 575
BsmI GAATGC 1 cut(s) 160
BsnI GGCC 3 cut(s) 195, 745, 761
BsoBI CYCGRG 1 cut(s) 1124
Bsp119I TTCGAA 1 cut(s) 137
Bsp1286I GDGCHC 1 cut(s) 1131
Bsp13I TCCGGA 1 cut(s) 628
Bsp143I GATC 2 cut(s) 117, 1346
BspACI CCGC 7 cut(s) 380, 414, 557, 593, 860, 1005, 1259
BspANI GGCC 3 cut(s) 195, 745, 761
BspCNI CTCAG 5 cut(s) 270, 286, 412, 517, 1078
BspEI TCCGGA 1 cut(s) 628
BspHI TCATGA 2 cut(s) 1099, 1252
BspLI GGNNCC 4 cut(s) 96, 633, 837, 885
BspMAI CTGCAG 3 cut(s) 103, 300, 1342
BspMI ACCTGC 1 cut(s) 1165
BspPI GGATC 2 cut(s) 112, 1354
BspT104I TTCGAA 1 cut(s) 137
BsrDI GCAATG 1 cut(s) 333
BsrFI RCCGGY 1 cut(s) 559
BsrI ACTGG 3 cut(s) 211, 965, 1025
BssAI RCCGGY 1 cut(s) 559
BssECI CCNNGG 2 cut(s) 121, 597
BssMI GATC 2 cut(s) 117, 1346
BssNI GRCGYC 2 cut(s) 70, 1416
BssT1I CCWWGG 1 cut(s) 597
Bst2UI CCWGG 6 cut(s) 123, 197, 518, 636, 813, 1179
Bst4CI ACNGT 5 cut(s) 115, 134, 475, 958, 1192
Bst6I CTCTTC 1 cut(s) 358
BstACI GRCGYC 2 cut(s) 70, 1416
BstAPI GCANNNNNTGC 1 cut(s) 1013
BstBAI YACGTR 1 cut(s) 77
BstBI TTCGAA 1 cut(s) 137
BstDEI CTNAG 5 cut(s) 273, 278, 399, 504, 1065
BstF5I GGATG 2 cut(s) 88, 1319
BstHHI GCGC 1 cut(s) 1091
BstKTI GATC 2 cut(s) 120, 1349
BstMAI GTCTC 1 cut(s) 255
BstMBI GATC 2 cut(s) 117, 1346
BstMWI GCNNNNNNNGC 5 cut(s) 767, 1004, 1013, 1157, 1166
BstNI CCWGG 6 cut(s) 123, 197, 518, 636, 813, 1179
BstPAI GACNNNNGTC 1 cut(s) 956
BstSCI CCNGG 6 cut(s) 121, 195, 516, 634, 811, 1177
BstSFI CTRYAG 3 cut(s) 99, 296, 1338
BstV1I GCAGC 8 cut(s) 712, 790, 800, 844, 1003, 1288, 1324, 1423
BstX2I RGATCY 1 cut(s) 1346
BstXI CCANNNNNNTGG 1 cut(s) 736
BstYI RGATCY 1 cut(s) 1346
Bsu36I CCTNAGG 1 cut(s) 1065
BsuRI GGCC 3 cut(s) 195, 745, 761
BtsCI GGATG 2 cut(s) 88, 1319
BtsIMutI CAGTG 2 cut(s) 204, 954
BveI ACCTGC 1 cut(s) 1165
CaiI CAGNNNCTG 2 cut(s) 128, 791
CciI TCATGA 2 cut(s) 1099, 1252
CfoI GCGC 1 cut(s) 1091
Cfr10I RCCGGY 1 cut(s) 559
Cfr13I GGNCC 3 cut(s) 125, 743, 794
CsiI ACCWGGT 1 cut(s) 811
Csp6I GTAC 1 cut(s) 162
CviAII CATG 6 cut(s) 613, 961, 1021, 1100, 1253, 1405
CviQI GTAC 1 cut(s) 162
DdeI CTNAG 5 cut(s) 273, 278, 399, 504, 1065
DpnI GATC 2 cut(s) 119, 1348
DpnII GATC 2 cut(s) 117, 1346
Eam1104I CTCTTC 1 cut(s) 358
EarI CTCTTC 1 cut(s) 358
EciI GGCGGA 2 cut(s) 403, 546
Ecl136II GAGCTC 1 cut(s) 1129
Eco130I CCWWGG 1 cut(s) 597
Eco24I GRGCYC 1 cut(s) 1131
Eco32I GATATC 1 cut(s) 239
Eco47I GGWCC 2 cut(s) 125, 794
Eco53kI GAGCTC 1 cut(s) 1129
Eco81I CCTNAGG 1 cut(s) 1065
Eco88I CYCGRG 1 cut(s) 1124
EcoICRI GAGCTC 1 cut(s) 1129
EcoO109I RGGNCCY 1 cut(s) 125
EcoRI GAATTC 1 cut(s) 1201
EcoRII CCWGG 6 cut(s) 121, 195, 516, 634, 811, 1177
EcoRV GATATC 1 cut(s) 239
EcoT14I CCWWGG 1 cut(s) 597
EcoT38I GRGCYC 1 cut(s) 1131
ErhI CCWWGG 1 cut(s) 597
FaeI CATG 6 cut(s) 616, 964, 1024, 1103, 1256, 1408
FaqI GGGAC 2 cut(s) 485, 575
FatI CATG 6 cut(s) 612, 960, 1020, 1099, 1252, 1404
FauI CCCGC 2 cut(s) 600, 853
Fnu4HI GCNGC 8 cut(s) 726, 789, 804, 858, 1017, 1302, 1338, 1437
FokI GGATG 2 cut(s) 95, 1306
FriOI GRGCYC 1 cut(s) 1131
Fsp4HI GCNGC 8 cut(s) 726, 789, 804, 858, 1017, 1302, 1338, 1437
FspBI CTAG 1 cut(s) 945
GlaI GCGC 1 cut(s) 1090
GluI GCNGC 8 cut(s) 726, 789, 804, 858, 1017, 1302, 1338, 1437
GsuI CTGGAG 4 cut(s) 306, 539, 731, 1203
HaeIII GGCC 3 cut(s) 195, 745, 761
HapII CCGG 3 cut(s) 560, 629, 1307
HhaI GCGC 1 cut(s) 1091
Hin1I GRCGYC 2 cut(s) 70, 1416
Hin1II CATG 6 cut(s) 616, 964, 1024, 1103, 1256, 1408
Hin6I GCGC 1 cut(s) 1089
HinP1I GCGC 1 cut(s) 1089
HpaII CCGG 3 cut(s) 560, 629, 1307
HphI GGTGA 2 cut(s) 466, 950
Hpy166II GTNNAC 5 cut(s) 68, 74, 568, 941, 1273
Hpy188I TCNGA 9 cut(s) 43, 246, 260, 427, 868, 908, 1081, 1324, 1390
Hpy188III TCNNGA 8 cut(s) 510, 629, 839, 1100, 1124, 1220, 1232, 1253
Hpy8I GTNNAC 5 cut(s) 68, 74, 568, 941, 1273
Hpy99I CGWCG 1 cut(s) 1421
HpyAV CCTTC 2 cut(s) 734, 1337
HpyCH4III ACNGT 5 cut(s) 115, 134, 475, 958, 1192
HpyCH4IV ACGT 5 cut(s) 64, 70, 76, 687, 1416
HpyF10VI GCNNNNNNNGC 5 cut(s) 767, 1004, 1013, 1157, 1166
HpyF3I CTNAG 5 cut(s) 273, 278, 399, 504, 1065
HpySE526I ACGT 5 cut(s) 64, 70, 76, 687, 1416
Hsp92I GRCGYC 2 cut(s) 70, 1416
Hsp92II CATG 6 cut(s) 616, 964, 1024, 1103, 1256, 1408
HspAI GCGC 1 cut(s) 1089
Kpn2I TCCGGA 1 cut(s) 628
Kzo9I GATC 2 cut(s) 117, 1346
LmnI GCTCC 5 cut(s) 100, 343, 841, 889, 1309
Lsp1109I GCAGC 8 cut(s) 712, 790, 800, 844, 1003, 1288, 1324, 1423
LweI GCATC 5 cut(s) 220, 574, 779, 887, 1246
MabI ACCWGGT 1 cut(s) 811
MaeI CTAG 1 cut(s) 945
MaeII ACGT 5 cut(s) 64, 70, 76, 687, 1416
MaeIII GTNAC 5 cut(s) 202, 522, 550, 949, 1309
MalI GATC 2 cut(s) 119, 1348
MboI GATC 2 cut(s) 117, 1346
MboII GAAGA 6 cut(s) 27, 151, 233, 375, 589, 1433
MfeI CAATTG 1 cut(s) 798
MflI RGATCY 1 cut(s) 1346
MhlI GDGCHC 1 cut(s) 1131
MlyI GAGTC 3 cut(s) 419, 530, 547
MmeI TCCRAC 1 cut(s) 238
MroI TCCGGA 1 cut(s) 628
MslI CAYNNNNRTG 4 cut(s) 209, 734, 959, 1104
MspA1I CMGCKG 3 cut(s) 277, 791, 1436
MspI CCGG 3 cut(s) 560, 629, 1307
MspR9I CCNGG 6 cut(s) 123, 197, 518, 636, 813, 1179
MunI CAATTG 1 cut(s) 798
Mva1269I GAATGC 1 cut(s) 160
MvaI CCWGG 6 cut(s) 123, 197, 518, 636, 813, 1179
MwoI GCNNNNNNNGC 5 cut(s) 767, 1004, 1013, 1157, 1166
NdeII GATC 2 cut(s) 117, 1346
NlaIII CATG 6 cut(s) 616, 964, 1024, 1103, 1256, 1408
NlaIV GGNNCC 4 cut(s) 96, 633, 837, 885
NmuCI GTSAC 4 cut(s) 202, 522, 550, 949
NspV TTCGAA 1 cut(s) 137
OliI CACNNNNGTG 1 cut(s) 209
PaeR7I CTCGAG 1 cut(s) 1124
PagI TCATGA 2 cut(s) 1099, 1252
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PctI GAATGC 1 cut(s) 160
PfeI GAWTC 8 cut(s) 4, 13, 513, 903, 1001, 1076, 1120, 1325
PfoI TCCNGGA 1 cut(s) 516
PkrI GCNGC 8 cut(s) 727, 790, 805, 859, 1018, 1303, 1339, 1438
PleI GAGTC 3 cut(s) 418, 529, 547
PpsI GAGTC 3 cut(s) 418, 529, 547
Ppu21I YACGTR 1 cut(s) 77
PpuMI RGGWCCY 1 cut(s) 125
PshAI GACNNNNGTC 1 cut(s) 956
Psp124BI GAGCTC 1 cut(s) 1131
Psp1406I AACGTT 1 cut(s) 687
Psp5II RGGWCCY 1 cut(s) 125
Psp6I CCWGG 6 cut(s) 121, 195, 516, 634, 811, 1177
PspGI CCWGG 6 cut(s) 121, 195, 516, 634, 811, 1177
PspN4I GGNNCC 4 cut(s) 96, 633, 837, 885
PspPI GGNCC 3 cut(s) 125, 743, 794
PspPPI RGGWCCY 1 cut(s) 125
PstI CTGCAG 3 cut(s) 103, 300, 1342
PstNI CAGNNNCTG 2 cut(s) 128, 791
PsuI RGATCY 1 cut(s) 1346
PvuII CAGCTG 3 cut(s) 277, 791, 1436
RsaI GTAC 1 cut(s) 163
RsaNI GTAC 1 cut(s) 162
RseI CAYNNNNRTG 4 cut(s) 209, 734, 959, 1104
SacI GAGCTC 1 cut(s) 1131
SatI GCNGC 8 cut(s) 726, 789, 804, 858, 1017, 1302, 1338, 1437
Sau3AI GATC 2 cut(s) 117, 1346
Sau96I GGNCC 3 cut(s) 125, 743, 794
SchI GAGTC 3 cut(s) 419, 530, 547
ScrFI CCNGG 6 cut(s) 123, 197, 518, 636, 813, 1179
SduI GDGCHC 1 cut(s) 1131
SexAI ACCWGGT 1 cut(s) 811
SfaNI GCATC 5 cut(s) 220, 574, 779, 887, 1246
SfcI CTRYAG 3 cut(s) 99, 296, 1338
Sfr274I CTCGAG 1 cut(s) 1124
SfuI TTCGAA 1 cut(s) 137
SinI GGWCC 2 cut(s) 125, 794
SlaI CTCGAG 1 cut(s) 1124
SmiMI CAYNNNNRTG 4 cut(s) 209, 734, 959, 1104
SmlI CTYRAG 1 cut(s) 1124
SmoI CTYRAG 1 cut(s) 1124
SsiI CCGC 7 cut(s) 380, 414, 557, 593, 860, 1005, 1259
SspMI CTAG 1 cut(s) 945
SstI GAGCTC 1 cut(s) 1131
StyD4I CCNGG 6 cut(s) 121, 195, 516, 634, 811, 1177
StyI CCWWGG 1 cut(s) 597
TaaI ACNGT 5 cut(s) 115, 134, 475, 958, 1192
TaiI ACGT 5 cut(s) 67, 73, 79, 690, 1419
TaqI TCGA 4 cut(s) 137, 1032, 1125, 1419
TatI WGTACW 1 cut(s) 161
TfiI GAWTC 8 cut(s) 4, 13, 513, 903, 1001, 1076, 1120, 1325
TscAI CASTG 2 cut(s) 211, 961
TseFI GTSAC 4 cut(s) 202, 522, 550, 949
TseI GCWGC 8 cut(s) 725, 788, 803, 857, 1016, 1301, 1337, 1436
Tsp45I GTSAC 4 cut(s) 202, 522, 550, 949
TspDTI ATGAA 8 cut(s) 17, 68, 99, 198, 906, 1088, 1233, 1241
TspRI CASTG 2 cut(s) 211, 961
VpaK11BI GGWCC 2 cut(s) 125, 794
XapI RAATTY 7 cut(s) 369, 486, 843, 849, 1056, 1201, 1209
XcmI CCANNNNNNNNNTGG 1 cut(s) 203
XhoI CTCGAG 1 cut(s) 1124
XspI CTAG 1 cut(s) 945
ZraI GACGTC 2 cut(s) 71, 1417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.