Rh5BG029800

DNA binding domain with preference for A/T rich regions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001372
Physical Location & Seq
Reverse (-)
2107692 .. 2109287
1596 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG029800.1

Sequence Viewer

Length: 1107 bp
ATGAAGCAGAAACGTGGACGTCCACGTAAGGATGAGAATTTAGTTCATGGAGAGAGCATGCCTGCAATACCAGGACCTGATAGTTTGAAGAAAAACAAGCAGAGTGCGGGTACTAACGATGATGTCGAAGATGAAATGGTGGGTCGGGTGGTTTCTGGTGTTATTGAAGGTTCATTTGATGCCGGCTATCTTCTGAATGTTGAGGTAGAAGACACAGACACTCAGCTGAGGGCTGTTGTATTTCTACCAGGAAAGTTCACTCCAGTCACTGCAGCAAATGATGTTGCTACTAATGTTAAGATGTGCAAAAGAAAAGAAGTTCCAATACCAGCTGTCAACCTGCAATCTCATCTTTATGGTTCTGCCCGGCCATCAGAGCAAAGTAACAGGCAACCTTCTGAGGTGAAAAAGCAAATACCTGCAGTTCCAGACCAAATTCCACCCCGTATTCCTCAGTGTGGCATCCCGGAGTCTCTTAGAAGTCAATCTTTCTCTGGCATCCCGGAGTCTCTTAGCCAACGCATCTCAGAGTCTCTTAGAAGTCGATCTTTCTCTGGCATCCCGGAGTCTCTTAGAAGCCAACCAACCTCTTTTGTAATTCCTCCGGTTGATAATTTGCCAAAAGATGATGCCCCTACCTTAGGAGCGTTGAAACTGTATGCAAGGCTCCTCAAATTCAACCTCAAGACTGTGGATACTGAAAAGAGTCAATTGGTTGATGATGAGGTAAAGAGTGTGAACGTTGAACTCAATCAAACTCCTGTATTTGCTGAACCTACACCAAGCTCTGTCATACTGGGAGTTGAGACTGTATGTAAGGATCCTCAAATTCAACCAGAGGATGTGGGTTCTGAAAAGAGTGCATTGGTTCAGGATGTGGTAAAGAGCCTAGATCTTGAACTCAACCAAACTCCTCTGGTTGCAGAACCTGCGAATCTGCCAGTAAACCAGTTGAGAGAATCTGATGGAAGAAGAGGCTGCACCAAAGCCGAGTCAGGTGTTAGGGGGAGATGCCATTCCACCAGCACAATCTTGGATCAGCTCCGTAGTACCAGGCTGGTTGGGCCGGTTTTTTCATTTCAGTCACAATTAATGCTTTTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

368

Amino Acids

40.13

Weight (kDa)

5.84

Isoelectric Point (pI)

58.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 22
Acc36I ACCTGC 3 cut(s) 348, 427, 937
AciI CCGC 1 cut(s) 107
AclI AACGTT 1 cut(s) 741
AclWI GGATC 3 cut(s) 815, 828, 1044
AcoI YGGCCR 1 cut(s) 368
AcsI RAATTY 4 cut(s) 37, 435, 674, 828
AcyI GRCGYC 1 cut(s) 19
AfaI GTAC 2 cut(s) 112, 1051
AfiI CCNNNNNNNGG 2 cut(s) 458, 641
AgsI TTSAA 7 cut(s) 88, 167, 652, 679, 746, 833, 899
AjnI CCWGG 3 cut(s) 70, 247, 1052
AluBI AGCT 4 cut(s) 226, 332, 786, 1042
AluI AGCT 4 cut(s) 226, 332, 786, 1042
Alw26I GTCTC 5 cut(s) 477, 513, 537, 573, 800
AlwI GGATC 3 cut(s) 815, 828, 1044
AlwNI CAGNNNCTG 3 cut(s) 77, 269, 929
AoxI GGCC 2 cut(s) 368, 1064
ApeKI GCWGC 2 cut(s) 272, 978
ApoI RAATTY 4 cut(s) 37, 435, 674, 828
AseI ATTAAT 1 cut(s) 1091
AspS9I GGNCC 2 cut(s) 74, 1064
AsuC2I CCSGG 4 cut(s) 367, 467, 503, 563
AsuHPI GGTGA 1 cut(s) 415
AvaII GGWCC 1 cut(s) 74
AxyI CCTNAGG 1 cut(s) 640
BamHI GGATCC 1 cut(s) 820
BarI GAAGNNNNNNTAC 2 cut(s) 309, 341
BbsI GAAGAC 1 cut(s) 216
BbvCI CCTCAGC 1 cut(s) 227
BbvI GCAGC 2 cut(s) 284, 965
BccI CCATC 2 cut(s) 379, 959
BciT130I CCWGG 3 cut(s) 72, 249, 1054
BciVI GTATCC 1 cut(s) 688
BcnI CCSGG 4 cut(s) 367, 467, 503, 563
BcoDI GTCTC 5 cut(s) 477, 513, 537, 573, 800
BfaI CTAG 1 cut(s) 890
BfmI CTRYAG 2 cut(s) 270, 420
BfuAI ACCTGC 3 cut(s) 348, 427, 937
BfuI GTATCC 1 cut(s) 688
BglII AGATCT 1 cut(s) 892
BisI GCNGC 2 cut(s) 273, 979
BlsI GCNGC 2 cut(s) 274, 980
Bme1390I CCNGG 7 cut(s) 72, 249, 367, 467, 503, 563, 1054
Bme18I GGWCC 1 cut(s) 74
BmgT120I GGNCC 2 cut(s) 74, 1064
BmiI GGNNCC 2 cut(s) 668, 822
BmrFI CCNGG 7 cut(s) 72, 249, 367, 467, 503, 563, 1054
BmrI ACTGGG 1 cut(s) 806
BmsI GCATC 7 cut(s) 169, 471, 507, 531, 567, 619, 1001
BmuI ACTGGG 1 cut(s) 806
BpiI GAAGAC 1 cut(s) 216
BpmI CTGGAG 1 cut(s) 246
Bpu10I CCTNAGC 1 cut(s) 227
BpuEI CTTGAG 1 cut(s) 668
BpuMI CCSGG 4 cut(s) 367, 467, 503, 563
BsaAI YACGTR 1 cut(s) 26
BsaHI GRCGYC 1 cut(s) 19
BsaWI WCCGGW 1 cut(s) 604
Bsc4I CCNNNNNNNGG 2 cut(s) 458, 641
Bse118I RCCGGY 2 cut(s) 182, 1066
Bse1I ACTGG 4 cut(s) 263, 801, 941, 949
Bse21I CCTNAGG 1 cut(s) 640
BseBI CCWGG 3 cut(s) 72, 249, 1054
BseGI GGATG 6 cut(s) 37, 462, 498, 558, 847, 880
BseLI CCNNNNNNNGG 2 cut(s) 458, 641
BseMII CTCAG 5 cut(s) 218, 236, 390, 467, 540
BseNI ACTGG 4 cut(s) 263, 801, 941, 949
BseRI GAGGAG 2 cut(s) 659, 903
BseXI GCAGC 2 cut(s) 284, 965
BsgI GTGCAG 1 cut(s) 964
BshFI GGCC 2 cut(s) 370, 1066
BsiSI CCGG 7 cut(s) 183, 367, 467, 503, 563, 605, 1067
BslI CCNNNNNNNGG 2 cut(s) 458, 641
BsmAI GTCTC 5 cut(s) 477, 513, 537, 573, 800
BsnI GGCC 2 cut(s) 370, 1066
Bsp143I GATC 4 cut(s) 545, 820, 892, 1036
BspACI CCGC 1 cut(s) 107
BspANI GGCC 2 cut(s) 370, 1066
BspCNI CTCAG 5 cut(s) 219, 235, 391, 466, 539
BspLI GGNNCC 2 cut(s) 668, 822
BspMAI CTGCAG 2 cut(s) 274, 424
BspMI ACCTGC 3 cut(s) 348, 427, 937
BspPI GGATC 3 cut(s) 815, 828, 1044
BsrFI RCCGGY 2 cut(s) 182, 1066
BsrI ACTGG 4 cut(s) 263, 801, 941, 949
BssAI RCCGGY 2 cut(s) 182, 1066
BssMI GATC 4 cut(s) 545, 820, 892, 1036
BssNI GRCGYC 1 cut(s) 19
Bst2UI CCWGG 3 cut(s) 72, 249, 1054
Bst4CI ACNGT 3 cut(s) 657, 691, 811
Bst6I CTCTTC 1 cut(s) 967
BstACI GRCGYC 1 cut(s) 19
BstAPI GCANNNNNTGC 1 cut(s) 929
BstBAI YACGTR 1 cut(s) 26
BstC8I GCNNGC 3 cut(s) 59, 63, 184
BstENI CCTNNNNNAGG 1 cut(s) 639
BstF5I GGATG 6 cut(s) 37, 462, 498, 558, 847, 880
BstKTI GATC 4 cut(s) 548, 823, 895, 1039
BstMAI GTCTC 5 cut(s) 477, 513, 537, 573, 800
BstMBI GATC 4 cut(s) 545, 820, 892, 1036
BstMWI GCNNNNNNNGC 3 cut(s) 376, 929, 1063
BstNI CCWGG 3 cut(s) 72, 249, 1054
BstNSI RCATGY 1 cut(s) 61
BstSCI CCNGG 7 cut(s) 70, 247, 365, 465, 501, 561, 1052
BstSFI CTRYAG 2 cut(s) 270, 420
BstV1I GCAGC 2 cut(s) 284, 965
BstV2I GAAGAC 1 cut(s) 216
BstX2I RGATCY 2 cut(s) 820, 892
BstYI RGATCY 2 cut(s) 820, 892
Bsu36I CCTNAGG 1 cut(s) 640
BsuI GTATCC 1 cut(s) 688
BsuRI GGCC 2 cut(s) 370, 1066
BtsCI GGATG 6 cut(s) 37, 462, 498, 558, 847, 880
BtsI GCAGTG 1 cut(s) 267
BtsIMutI CAGTG 2 cut(s) 267, 461
BveI ACCTGC 3 cut(s) 348, 427, 937
Cac8I GCNNGC 3 cut(s) 59, 63, 184
CaiI CAGNNNCTG 3 cut(s) 77, 269, 929
Cfr10I RCCGGY 2 cut(s) 182, 1066
Cfr13I GGNCC 2 cut(s) 74, 1064
Csp6I GTAC 2 cut(s) 111, 1050
CviAII CATG 2 cut(s) 47, 58
CviQI GTAC 2 cut(s) 111, 1050
DpnI GATC 4 cut(s) 547, 822, 894, 1038
DpnII GATC 4 cut(s) 545, 820, 892, 1036
EaeI YGGCCR 1 cut(s) 368
Eam1104I CTCTTC 1 cut(s) 967
EarI CTCTTC 1 cut(s) 967
Eco47I GGWCC 1 cut(s) 74
Eco81I CCTNAGG 1 cut(s) 640
EcoNI CCTNNNNNAGG 1 cut(s) 639
EcoO109I RGGNCCY 1 cut(s) 74
EcoRII CCWGG 3 cut(s) 70, 247, 1052
FaeI CATG 2 cut(s) 50, 61
FaiI YATR 6 cut(s) 48, 59, 357, 660, 794, 814
FalI AAGNNNNNCTT 4 cut(s) 472, 504, 532, 564
FatI CATG 2 cut(s) 46, 57
FauI CCCGC 1 cut(s) 100
Fnu4HI GCNGC 2 cut(s) 273, 979
FokI GGATG 6 cut(s) 44, 449, 485, 545, 854, 887
Fsp4HI GCNGC 2 cut(s) 273, 979
FspBI CTAG 1 cut(s) 890
GluI GCNGC 2 cut(s) 273, 979
GsuI CTGGAG 1 cut(s) 246
HaeIII GGCC 2 cut(s) 370, 1066
HapII CCGG 7 cut(s) 183, 367, 467, 503, 563, 605, 1067
Hin1I GRCGYC 1 cut(s) 19
Hin1II CATG 2 cut(s) 50, 61
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HinfI GANTC 8 cut(s) 470, 506, 530, 566, 706, 934, 959, 992
HpaII CCGG 7 cut(s) 183, 367, 467, 503, 563, 605, 1067
HphI GGTGA 1 cut(s) 415
Hpy166II GTNNAC 6 cut(s) 17, 23, 258, 337, 739, 946
Hpy188I TCNGA 6 cut(s) 195, 376, 400, 529, 853, 964
Hpy188III TCNNGA 4 cut(s) 428, 685, 872, 896
Hpy8I GTNNAC 6 cut(s) 17, 23, 258, 337, 739, 946
HpyAV CCTTC 2 cut(s) 161, 405
HpyCH4III ACNGT 3 cut(s) 657, 691, 811
HpyCH4IV ACGT 4 cut(s) 13, 19, 25, 741
HpyCH4V TGCA 9 cut(s) 65, 272, 306, 343, 422, 662, 863, 923, 981
HpyF10VI GCNNNNNNNGC 3 cut(s) 376, 929, 1063
HpySE526I ACGT 4 cut(s) 13, 19, 25, 741
Hsp92I GRCGYC 1 cut(s) 19
Hsp92II CATG 2 cut(s) 50, 61
KroI GCCGGC 1 cut(s) 182
KroNI GCCGGC 1 cut(s) 184
Kzo9I GATC 4 cut(s) 545, 820, 892, 1036
LmnI GCTCC 3 cut(s) 644, 672, 1047
Lsp1109I GCAGC 2 cut(s) 284, 965
LweI GCATC 7 cut(s) 169, 471, 507, 531, 567, 619, 1001
MaeI CTAG 1 cut(s) 890
MaeII ACGT 4 cut(s) 13, 19, 25, 741
MaeIII GTNAC 3 cut(s) 265, 383, 1083
MalI GATC 4 cut(s) 547, 822, 894, 1038
MboI GATC 4 cut(s) 545, 820, 892, 1036
MboII GAAGA 6 cut(s) 100, 140, 182, 221, 981, 984
MfeI CAATTG 1 cut(s) 710
MflI RGATCY 2 cut(s) 820, 892
MluCI AATT 8 cut(s) 37, 435, 597, 613, 674, 710, 828, 1088
MlyI GAGTC 6 cut(s) 479, 515, 539, 575, 715, 1001
MroNI GCCGGC 1 cut(s) 182
MseI TTAA 2 cut(s) 297, 1091
MslI CAYNNNNRTG 1 cut(s) 354
MspA1I CMGCKG 2 cut(s) 226, 332
MspI CCGG 7 cut(s) 183, 367, 467, 503, 563, 605, 1067
MspR9I CCNGG 7 cut(s) 72, 249, 367, 467, 503, 563, 1054
MunI CAATTG 1 cut(s) 710
MvaI CCWGG 3 cut(s) 72, 249, 1054
MwoI GCNNNNNNNGC 3 cut(s) 376, 929, 1063
NaeI GCCGGC 1 cut(s) 184
NciI CCSGG 4 cut(s) 367, 467, 503, 563
NdeII GATC 4 cut(s) 545, 820, 892, 1036
NgoMIV GCCGGC 1 cut(s) 182
NlaIII CATG 2 cut(s) 50, 61
NlaIV GGNNCC 2 cut(s) 668, 822
NmeAIII GCCGAG 1 cut(s) 1015
NmuCI GTSAC 2 cut(s) 265, 1083
NspI RCATGY 1 cut(s) 61
PaeI GCATGC 1 cut(s) 61
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PdiI GCCGGC 1 cut(s) 184
PfeI GAWTC 2 cut(s) 934, 959
PfoI TCCNGGA 3 cut(s) 465, 501, 561
PkrI GCNGC 2 cut(s) 274, 980
PleI GAGTC 6 cut(s) 478, 514, 538, 574, 714, 1000
PpsI GAGTC 6 cut(s) 478, 514, 538, 574, 714, 1000
Ppu21I YACGTR 1 cut(s) 26
PpuMI RGGWCCY 1 cut(s) 74
PshBI ATTAAT 1 cut(s) 1091
Psp1406I AACGTT 1 cut(s) 741
Psp5II RGGWCCY 1 cut(s) 74
Psp6I CCWGG 3 cut(s) 70, 247, 1052
PspGI CCWGG 3 cut(s) 70, 247, 1052
PspN4I GGNNCC 2 cut(s) 668, 822
PspPI GGNCC 2 cut(s) 74, 1064
PspPPI RGGWCCY 1 cut(s) 74
PstI CTGCAG 2 cut(s) 274, 424
PstNI CAGNNNCTG 3 cut(s) 77, 269, 929
PsuI RGATCY 2 cut(s) 820, 892
PvuII CAGCTG 2 cut(s) 226, 332
RsaI GTAC 2 cut(s) 112, 1051
RsaNI GTAC 2 cut(s) 111, 1050
RseI CAYNNNNRTG 1 cut(s) 354
SaqAI TTAA 2 cut(s) 297, 1091
SatI GCNGC 2 cut(s) 273, 979
Sau3AI GATC 4 cut(s) 545, 820, 892, 1036
Sau96I GGNCC 2 cut(s) 74, 1064
SchI GAGTC 6 cut(s) 479, 515, 539, 575, 715, 1001
ScrFI CCNGG 7 cut(s) 72, 249, 367, 467, 503, 563, 1054
SfaNI GCATC 7 cut(s) 169, 471, 507, 531, 567, 619, 1001
SfcI CTRYAG 2 cut(s) 270, 420
SinI GGWCC 1 cut(s) 74
SmiMI CAYNNNNRTG 1 cut(s) 354
SmlI CTYRAG 1 cut(s) 683
SmoI CTYRAG 1 cut(s) 683
SphI GCATGC 1 cut(s) 61
Sse9I AATT 8 cut(s) 37, 435, 597, 613, 674, 710, 828, 1088
SsiI CCGC 1 cut(s) 107
SspMI CTAG 1 cut(s) 890
StyD4I CCNGG 7 cut(s) 70, 247, 365, 465, 501, 561, 1052
TaaI ACNGT 3 cut(s) 657, 691, 811
TaiI ACGT 4 cut(s) 16, 22, 28, 744
TaqI TCGA 2 cut(s) 126, 544
TasI AATT 8 cut(s) 37, 435, 597, 613, 674, 710, 828, 1088
TfiI GAWTC 2 cut(s) 934, 959
Tru1I TTAA 2 cut(s) 297, 1091
Tru9I TTAA 2 cut(s) 297, 1091
TscAI CASTG 2 cut(s) 274, 461
TseFI GTSAC 2 cut(s) 265, 1083
TseI GCWGC 2 cut(s) 272, 978
Tsp45I GTSAC 2 cut(s) 265, 1083
TspDTI ATGAA 5 cut(s) 17, 35, 147, 162, 1065
TspGWI ACGGA 1 cut(s) 1034
TspRI CASTG 2 cut(s) 274, 461
VpaK11BI GGWCC 1 cut(s) 74
VspI ATTAAT 1 cut(s) 1091
XagI CCTNNNNNAGG 1 cut(s) 639
XapI RAATTY 4 cut(s) 37, 435, 674, 828
XceI RCATGY 1 cut(s) 61
XcmI CCANNNNNNNNNTGG 1 cut(s) 1030
XspI CTAG 1 cut(s) 890
ZraI GACGTC 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.