Rh5BG029700

DNA binding domain with preference for A/T rich regions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001372
Physical Location & Seq
Forward (+)
2104572 .. 2106101
1530 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG029700.1

Sequence Viewer

Length: 1530 bp
ATGAATCAGAGTCAAGGAACAAGCTCTTTATCTTCGCCAGATCTCCCTATGAAGCGGAAACGTGGACGTCCACGAAAGGATGAGAATTTAGTTCACGGAGAGAGCATGCCTGCAATGCCAGGACCTGATAGTTTGAAGAAAAACAAACAGAGTGCGGGTACTAACGATGATGGTGAAGATGAAATGGTGGGTCGAGTGGTTTCTGGTGTTATTGAAGGTTCATTTGATGCCGGCTATCTTCTGAATGTTAAGGTAGAAGACACCAACACTCAGCTGAGGGGTGTTGTATTTCTTCCGGGAAAGTTCACTCCAGTCACTGCAGCAAATGATGTTGCTCCTAATGTTAAGATGTGCAAAAGAAAAGAAGTTCCAATACCAGCTGTCAACCTGCAATCTCATCTTTATGGTTCTGCCCGGCCATCAGAGCAAAGTAACAGGCCACCTGTTGAGGTGAAAAAGCAAATACCTGCAATTCCAGACCAAATTCCTCCCCGTATTCCTCAGTGTGGCATCCCGGAGACTCTTAGAAGTCAATCTTTCTCTGGCATCCCAGAGTCTCTTCAAAGCCAACGCATCCAGGAGTCTCTTAGAAGTCAATCTTTCCCTGGCATCCCGGAGTCTCTTAGCCAACCATCCTCTTTTGTAAATCCTCCGGTTGATAATTTGCCAAAGAATGATGCCCCTGTCTTGGGAGGTAAAGTCACACCACAGCAACCTTTGGATCCTGGACTTGATAACCAATCCACCCATGTAATGACAAAGTCCGAGAATGATGAAGTTGTTGAACAGGAAAAAGTGTTGGGGGAAGTTGAACCGGCCACAACAATAACAGAGCCAACTGGTGTAGAGGACAATAAAGAATCAAAGACAGAAACATCATCTGAACCAATTGGTGAGGTACTAAGCATAGACACAGTATGTAAGGATCCTCCAATTCAACCTCAGGCTGTGGGTTCTGAAAGCAGTGCATTGGTTCAGGACGACGTAAAGAGCATGAATTTTGAGTCAATTCCTGTATTTGCTGAAGCTGCTCCTGCAATGGCTACCATACAAAGCGTTGAAACTGTATGCAAGGCTCCTCAAATTCAACCTCAGACTTTGGATACTGAAAAGAGTCAATTGGTTGATGATGAGGTAAAGAGTGTGAACGTTGAACTCAATCAAACTCCTGTATTTGCTGAACCCGCAACCAGTTCTGTCATACTGGGAGTTGAGACTGTATGTAAGGATCCTCAAATTCAACCTGAGGATGTGGGTTCTGAAAAGAGTGCATTGGTTCAGGATGTGGTAAAGAGCCTACATCTTGAGCTCAACCAAACTCCTCTGGTTGCAGAACCTGCATTTGCAGCCAGTAAACCAGTTGACAATCTGATGGAAGAAGAGGCTGCACCAAAGCCGAGTCAGGTGTTAGGGGGAGGTACTGTTGCATCTGTAGGATCCATTCCTTCAGCCATGGGTGAACCTCCTTCTGGTGCTGTTGACAGTATGGAATGTGATATTGCAGATGCCATTTCACCTGCACAATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

509

Amino Acids

54.09

Weight (kDa)

4.53

Isoelectric Point (pI)

61.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1525
AatII GACGTC 1 cut(s) 70
Acc36I ACCTGC 4 cut(s) 396, 475, 1345, 1525
AciI CCGC 3 cut(s) 55, 155, 1185
AclI AACGTT 1 cut(s) 1149
AclWI GGATC 8 cut(s) 716, 729, 920, 933, 1223, 1236, 1431, 1444
AcoI YGGCCR 2 cut(s) 416, 816
AcsI RAATTY 5 cut(s) 85, 483, 997, 1083, 1236
AcuI CTGAAG 2 cut(s) 1044, 1431
AcyI GRCGYC 1 cut(s) 67
AfaI GTAC 3 cut(s) 160, 900, 1420
AfiI CCNNNNNNNGG 4 cut(s) 506, 688, 689, 1469
AjnI CCWGG 4 cut(s) 118, 576, 604, 724
AluBI AGCT 5 cut(s) 24, 274, 380, 1028, 1309
AluI AGCT 5 cut(s) 24, 274, 380, 1028, 1309
Alw21I GWGCWC 1 cut(s) 1311
Alw26I GTCTC 5 cut(s) 512, 561, 588, 624, 1208
AlwI GGATC 8 cut(s) 716, 729, 920, 933, 1223, 1236, 1431, 1444
AlwNI CAGNNNCTG 3 cut(s) 125, 317, 1337
AoxI GGCC 3 cut(s) 416, 437, 816
ApeKI GCWGC 4 cut(s) 320, 1028, 1346, 1385
ApoI RAATTY 5 cut(s) 85, 483, 997, 1083, 1236
AspS9I GGNCC 1 cut(s) 122
AsuC2I CCSGG 4 cut(s) 297, 415, 515, 614
AsuHPI GGTGA 5 cut(s) 185, 463, 905, 1469, 1506
AvaII GGWCC 1 cut(s) 122
AxyI CCTNAGG 2 cut(s) 942, 1245
BamHI GGATCC 4 cut(s) 721, 925, 1228, 1436
BanII GRGCYC 1 cut(s) 1311
BarI GAAGNNNNNNTAC 2 cut(s) 357, 389
BbsI GAAGAC 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 1311
BbvCI CCTCAGC 1 cut(s) 275
BbvI GCAGC 4 cut(s) 332, 1015, 1358, 1372
BccI CCATC 4 cut(s) 164, 427, 640, 1366
BciT130I CCWGG 4 cut(s) 120, 578, 606, 726
BciVI GTATCC 1 cut(s) 1096
BcnI CCSGG 4 cut(s) 297, 415, 515, 614
BcoDI GTCTC 5 cut(s) 512, 561, 588, 624, 1208
BfmI CTRYAG 2 cut(s) 318, 1431
BfuAI ACCTGC 4 cut(s) 396, 475, 1345, 1525
BfuI GTATCC 1 cut(s) 1096
BglII AGATCT 1 cut(s) 40
BisI GCNGC 4 cut(s) 321, 1029, 1347, 1386
BlsI GCNGC 4 cut(s) 322, 1030, 1348, 1387
Bme1390I CCNGG 8 cut(s) 120, 297, 415, 515, 578, 606, 614, 726
Bme18I GGWCC 1 cut(s) 122
BmgT120I GGNCC 1 cut(s) 122
BmiI GGNNCC 5 cut(s) 723, 927, 1077, 1230, 1438
BmrFI CCNGG 8 cut(s) 120, 297, 415, 515, 578, 606, 614, 726
BmrI ACTGGG 1 cut(s) 1214
BmsI GCATC 8 cut(s) 217, 519, 555, 582, 618, 667, 1436, 1495
BmuI ACTGGG 1 cut(s) 1214
BpiI GAAGAC 1 cut(s) 264
BpmI CTGGAG 1 cut(s) 294
Bpu10I CCTNAGC 1 cut(s) 275
BpuEI CTTGAG 1 cut(s) 1325
BpuMI CCSGG 4 cut(s) 297, 415, 515, 614
BsaHI GRCGYC 1 cut(s) 67
BsaJI CCNNGG 2 cut(s) 604, 1452
BsaWI WCCGGW 1 cut(s) 652
BsaXI ACNNNNNCTCC 4 cut(s) 684, 714, 1407, 1437
Bsc4I CCNNNNNNNGG 4 cut(s) 506, 688, 689, 1469
Bse118I RCCGGY 2 cut(s) 230, 814
Bse1I ACTGG 6 cut(s) 311, 844, 1191, 1209, 1350, 1358
Bse21I CCTNAGG 2 cut(s) 942, 1245
Bse3DI GCAATG 2 cut(s) 120, 1044
BseBI CCWGG 4 cut(s) 120, 578, 606, 726
BseDI CCNNGG 2 cut(s) 604, 1452
BseGI GGATG 8 cut(s) 85, 510, 546, 573, 609, 632, 1255, 1288
BseLI CCNNNNNNNGG 4 cut(s) 506, 688, 689, 1469
BseMI GCAATG 2 cut(s) 120, 1044
BseMII CTCAG 6 cut(s) 266, 284, 515, 956, 1106, 1236
BseNI ACTGG 6 cut(s) 311, 844, 1191, 1209, 1350, 1358
BseRI GAGGAG 2 cut(s) 1068, 1311
BseXI GCAGC 4 cut(s) 332, 1015, 1358, 1372
BsgI GTGCAG 2 cut(s) 1371, 1503
BshFI GGCC 3 cut(s) 418, 439, 818
BsiHKAI GWGCWC 1 cut(s) 1311
BsiSI CCGG 7 cut(s) 231, 296, 415, 515, 614, 653, 815
BslI CCNNNNNNNGG 4 cut(s) 506, 688, 689, 1469
BsmAI GTCTC 5 cut(s) 512, 561, 588, 624, 1208
BsnI GGCC 3 cut(s) 418, 439, 818
Bsp1286I GDGCHC 1 cut(s) 1311
Bsp143I GATC 5 cut(s) 40, 721, 925, 1228, 1436
Bsp19I CCATGG 1 cut(s) 1452
BspACI CCGC 3 cut(s) 55, 155, 1185
BspANI GGCC 3 cut(s) 418, 439, 818
BspCNI CTCAG 6 cut(s) 267, 283, 514, 955, 1105, 1237
BspLI GGNNCC 5 cut(s) 723, 927, 1077, 1230, 1438
BspMAI CTGCAG 1 cut(s) 322
BspMI ACCTGC 4 cut(s) 396, 475, 1345, 1525
BspPI GGATC 8 cut(s) 716, 729, 920, 933, 1223, 1236, 1431, 1444
BsrDI GCAATG 2 cut(s) 120, 1044
BsrFI RCCGGY 2 cut(s) 230, 814
BsrI ACTGG 6 cut(s) 311, 844, 1191, 1209, 1350, 1358
BssAI RCCGGY 2 cut(s) 230, 814
BssECI CCNNGG 2 cut(s) 604, 1452
BssMI GATC 5 cut(s) 40, 721, 925, 1228, 1436
BssNI GRCGYC 1 cut(s) 67
BssT1I CCWWGG 1 cut(s) 1452
Bst2UI CCWGG 4 cut(s) 120, 578, 606, 726
Bst4CI ACNGT 5 cut(s) 916, 1066, 1219, 1423, 1484
Bst6I CTCTTC 2 cut(s) 564, 1374
BstACI GRCGYC 1 cut(s) 67
BstAPI GCANNNNNTGC 1 cut(s) 1337
BstC8I GCNNGC 3 cut(s) 107, 111, 232
BstDSI CCRYGG 1 cut(s) 1452
BstF5I GGATG 8 cut(s) 85, 510, 546, 573, 609, 632, 1255, 1288
BstKTI GATC 5 cut(s) 43, 724, 928, 1231, 1439
BstMAI GTCTC 5 cut(s) 512, 561, 588, 624, 1208
BstMBI GATC 5 cut(s) 40, 721, 925, 1228, 1436
BstMWI GCNNNNNNNGC 7 cut(s) 115, 424, 1028, 1034, 1184, 1337, 1346
BstNI CCWGG 4 cut(s) 120, 578, 606, 726
BstNSI RCATGY 1 cut(s) 109
BstSCI CCNGG 8 cut(s) 118, 295, 413, 513, 576, 604, 612, 724
BstSFI CTRYAG 2 cut(s) 318, 1431
BstV1I GCAGC 4 cut(s) 332, 1015, 1358, 1372
BstV2I GAAGAC 1 cut(s) 264
BstX2I RGATCY 5 cut(s) 40, 721, 925, 1228, 1436
BstYI RGATCY 5 cut(s) 40, 721, 925, 1228, 1436
Bsu36I CCTNAGG 2 cut(s) 942, 1245
BsuI GTATCC 1 cut(s) 1096
BsuRI GGCC 3 cut(s) 418, 439, 818
BtgI CCRYGG 1 cut(s) 1452
BtsCI GGATG 8 cut(s) 85, 510, 546, 573, 609, 632, 1255, 1288
BtsI GCAGTG 2 cut(s) 315, 970
BtsIMutI CAGTG 3 cut(s) 315, 509, 970
BveI ACCTGC 4 cut(s) 396, 475, 1345, 1525
Cac8I GCNNGC 3 cut(s) 107, 111, 232
CaiI CAGNNNCTG 3 cut(s) 125, 317, 1337
Cfr10I RCCGGY 2 cut(s) 230, 814
Cfr13I GGNCC 1 cut(s) 122
Csp6I GTAC 3 cut(s) 159, 899, 1419
CviAII CATG 4 cut(s) 106, 749, 994, 1453
CviQI GTAC 3 cut(s) 159, 899, 1419
DpnI GATC 5 cut(s) 42, 723, 927, 1230, 1438
DpnII GATC 5 cut(s) 40, 721, 925, 1228, 1436
EaeI YGGCCR 2 cut(s) 416, 816
Eam1104I CTCTTC 2 cut(s) 564, 1374
EarI CTCTTC 2 cut(s) 564, 1374
Ecl136II GAGCTC 1 cut(s) 1309
Eco130I CCWWGG 1 cut(s) 1452
Eco24I GRGCYC 1 cut(s) 1311
Eco47I GGWCC 1 cut(s) 122
Eco53kI GAGCTC 1 cut(s) 1309
Eco57I CTGAAG 2 cut(s) 1044, 1431
Eco81I CCTNAGG 2 cut(s) 942, 1245
EcoICRI GAGCTC 1 cut(s) 1309
EcoO109I RGGNCCY 1 cut(s) 122
EcoRII CCWGG 4 cut(s) 118, 576, 604, 724
EcoT14I CCWWGG 1 cut(s) 1452
EcoT38I GRGCYC 1 cut(s) 1311
ErhI CCWWGG 1 cut(s) 1452
FaeI CATG 4 cut(s) 109, 752, 997, 1456
FalI AAGNNNNNCTT 4 cut(s) 520, 552, 583, 615
FatI CATG 4 cut(s) 105, 748, 993, 1452
FauI CCCGC 2 cut(s) 148, 1192
Fnu4HI GCNGC 4 cut(s) 321, 1029, 1347, 1386
FokI GGATG 8 cut(s) 92, 497, 533, 560, 596, 619, 1262, 1295
FriOI GRGCYC 1 cut(s) 1311
Fsp4HI GCNGC 4 cut(s) 321, 1029, 1347, 1386
GluI GCNGC 4 cut(s) 321, 1029, 1347, 1386
GsuI CTGGAG 1 cut(s) 294
HaeIII GGCC 3 cut(s) 418, 439, 818
HapII CCGG 7 cut(s) 231, 296, 415, 515, 614, 653, 815
Hin1I GRCGYC 1 cut(s) 67
Hin1II CATG 4 cut(s) 109, 752, 997, 1456
HincII GTYRAC 3 cut(s) 385, 1363, 1480
HindII GTYRAC 3 cut(s) 385, 1363, 1480
HpaII CCGG 7 cut(s) 231, 296, 415, 515, 614, 653, 815
HphI GGTGA 5 cut(s) 185, 463, 905, 1469, 1506
Hpy188I TCNGA 9 cut(s) 9, 243, 424, 766, 883, 958, 1095, 1261, 1371
Hpy188III TCNNGA 4 cut(s) 476, 977, 1280, 1304
Hpy99I CGWCG 1 cut(s) 986
HpyAV CCTTC 3 cut(s) 209, 1455, 1476
HpyCH4III ACNGT 5 cut(s) 916, 1066, 1219, 1423, 1484
HpyCH4IV ACGT 4 cut(s) 61, 67, 984, 1149
HpyF10VI GCNNNNNNNGC 7 cut(s) 115, 424, 1028, 1034, 1184, 1337, 1346
HpySE526I ACGT 4 cut(s) 61, 67, 984, 1149
Hsp92I GRCGYC 1 cut(s) 67
Hsp92II CATG 4 cut(s) 109, 752, 997, 1456
KroI GCCGGC 1 cut(s) 230
KroNI GCCGGC 1 cut(s) 232
Kzo9I GATC 5 cut(s) 40, 721, 925, 1228, 1436
LmnI GCTCC 3 cut(s) 340, 1036, 1081
Lsp1109I GCAGC 4 cut(s) 332, 1015, 1358, 1372
LweI GCATC 8 cut(s) 217, 519, 555, 582, 618, 667, 1436, 1495
MaeII ACGT 4 cut(s) 61, 67, 984, 1149
MaeIII GTNAC 3 cut(s) 313, 431, 700
MalI GATC 5 cut(s) 42, 723, 927, 1230, 1438
MboI GATC 5 cut(s) 40, 721, 925, 1228, 1436
MboII GAAGA 9 cut(s) 24, 148, 188, 230, 269, 284, 551, 1388, 1391
MfeI CAATTG 2 cut(s) 888, 1118
MflI RGATCY 5 cut(s) 40, 721, 925, 1228, 1436
MhlI GDGCHC 1 cut(s) 1311
MlyI GAGTC 8 cut(s) 19, 514, 563, 590, 626, 1013, 1123, 1408
MroNI GCCGGC 1 cut(s) 230
MseI TTAA 2 cut(s) 249, 345
MslI CAYNNNNRTG 1 cut(s) 402
MspA1I CMGCKG 2 cut(s) 274, 380
MspI CCGG 7 cut(s) 231, 296, 415, 515, 614, 653, 815
MspR9I CCNGG 8 cut(s) 120, 297, 415, 515, 578, 606, 614, 726
MunI CAATTG 2 cut(s) 888, 1118
MvaI CCWGG 4 cut(s) 120, 578, 606, 726
MwoI GCNNNNNNNGC 7 cut(s) 115, 424, 1028, 1034, 1184, 1337, 1346
NaeI GCCGGC 1 cut(s) 232
NciI CCSGG 4 cut(s) 297, 415, 515, 614
NcoI CCATGG 1 cut(s) 1452
NdeII GATC 5 cut(s) 40, 721, 925, 1228, 1436
NgoMIV GCCGGC 1 cut(s) 230
NlaIII CATG 4 cut(s) 109, 752, 997, 1456
NlaIV GGNNCC 5 cut(s) 723, 927, 1077, 1230, 1438
NmeAIII GCCGAG 1 cut(s) 1422
NmuCI GTSAC 2 cut(s) 313, 700
NspI RCATGY 1 cut(s) 109
PaeI GCATGC 1 cut(s) 109
PaqCI CACCTGC 1 cut(s) 1525
PdiI GCCGGC 1 cut(s) 232
PfeI GAWTC 2 cut(s) 4, 860
PflFI GACNNNGTC 1 cut(s) 760
PfoI TCCNGGA 5 cut(s) 295, 513, 576, 612, 724
PkrI GCNGC 4 cut(s) 322, 1030, 1348, 1387
PleI GAGTC 8 cut(s) 18, 514, 562, 589, 625, 1012, 1122, 1407
PpsI GAGTC 8 cut(s) 18, 514, 562, 589, 625, 1012, 1122, 1407
PpuMI RGGWCCY 1 cut(s) 122
Psp124BI GAGCTC 1 cut(s) 1311
Psp1406I AACGTT 1 cut(s) 1149
Psp5II RGGWCCY 1 cut(s) 122
Psp6I CCWGG 4 cut(s) 118, 576, 604, 724
PspGI CCWGG 4 cut(s) 118, 576, 604, 724
PspN4I GGNNCC 5 cut(s) 723, 927, 1077, 1230, 1438
PspPI GGNCC 1 cut(s) 122
PspPPI RGGWCCY 1 cut(s) 122
PstI CTGCAG 1 cut(s) 322
PstNI CAGNNNCTG 3 cut(s) 125, 317, 1337
PsuI RGATCY 5 cut(s) 40, 721, 925, 1228, 1436
PsyI GACNNNGTC 1 cut(s) 760
PvuII CAGCTG 2 cut(s) 274, 380
RsaI GTAC 3 cut(s) 160, 900, 1420
RsaNI GTAC 3 cut(s) 159, 899, 1419
RseI CAYNNNNRTG 1 cut(s) 402
SacI GAGCTC 1 cut(s) 1311
SaqAI TTAA 2 cut(s) 249, 345
SatI GCNGC 4 cut(s) 321, 1029, 1347, 1386
Sau3AI GATC 5 cut(s) 40, 721, 925, 1228, 1436
Sau96I GGNCC 1 cut(s) 122
SchI GAGTC 8 cut(s) 19, 514, 563, 590, 626, 1013, 1123, 1408
ScrFI CCNGG 8 cut(s) 120, 297, 415, 515, 578, 606, 614, 726
SduI GDGCHC 1 cut(s) 1311
SfaNI GCATC 8 cut(s) 217, 519, 555, 582, 618, 667, 1436, 1495
SfcI CTRYAG 2 cut(s) 318, 1431
SinI GGWCC 1 cut(s) 122
SmiMI CAYNNNNRTG 1 cut(s) 402
SmlI CTYRAG 1 cut(s) 1304
SmoI CTYRAG 1 cut(s) 1304
SphI GCATGC 1 cut(s) 109
SsiI CCGC 3 cut(s) 55, 155, 1185
SstI GAGCTC 1 cut(s) 1311
StyD4I CCNGG 8 cut(s) 118, 295, 413, 513, 576, 604, 612, 724
StyI CCWWGG 1 cut(s) 1452
TaaI ACNGT 5 cut(s) 916, 1066, 1219, 1423, 1484
TaiI ACGT 4 cut(s) 64, 70, 987, 1152
TaqI TCGA 1 cut(s) 193
TfiI GAWTC 2 cut(s) 4, 860
Tru1I TTAA 2 cut(s) 249, 345
Tru9I TTAA 2 cut(s) 249, 345
TscAI CASTG 3 cut(s) 322, 509, 970
TseFI GTSAC 2 cut(s) 313, 700
TseI GCWGC 4 cut(s) 320, 1028, 1346, 1385
Tsp45I GTSAC 2 cut(s) 313, 700
TspDTI ATGAA 6 cut(s) 17, 65, 195, 210, 789, 1010
TspGWI ACGGA 1 cut(s) 111
TspRI CASTG 3 cut(s) 322, 509, 970
Tth111I GACNNNGTC 1 cut(s) 760
VpaK11BI GGWCC 1 cut(s) 122
XapI RAATTY 5 cut(s) 85, 483, 997, 1083, 1236
XceI RCATGY 1 cut(s) 109
ZraI GACGTC 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.