Rh5BG030000

DNA binding domain with preference for A/T rich regions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001372
Physical Location & Seq
Forward (+)
2122658 .. 2123227
570 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG030000.1

Sequence Viewer

Length: 570 bp
ATGCCACTTTTCAGGATTGCGAGTACAACTACCTATATTGATGCTTGCTTCAGGACTCTTCGTGATGAGTTGGTGCTTCACATATCTAACTTCGGGGTTCCTTCGACCACTTCTCATGGTGGAGATGATCACAACATGAATATTGATGGAGAGGACCATGGAGGTTACAATGAACAGAGTCAGTTGGTTGATGATGAGGTCAGGAGCCCGAACCTTGAACTTAGTCAAACTCCTGTATTTGGTGCACATGCACCAAGTTCTATCATGCTGGGAGGTGAGACTGTGTGCAAGGATCCTCAAATTCAACCTGATGATGTAGATTCTGAAAAGAGTGCATTGGTTCAGGATGTGATAAAGAACCTCGATCTCGAACTCAACCTATTTGCAGAGCCTACATTCGCTGCTCGTGATCCAACCAGTATAGAAGAAGAGCCTGCACCAAAGCCAAGCGAGGTGTTCGGGGGAGAGACTGTTGCATCTGAAGGATCCATTCCTTCAGGAACAATCGAACCAGGCAATCTGGTACTGTTAGACACTATGGCATGTGATATTGGAGAGGTGGAATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

189

Amino Acids

20.29

Weight (kDa)

4.08

Isoelectric Point (pI)

59.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 5 cut(s) 287, 300, 404, 480, 493
AcsI RAATTY 1 cut(s) 300
AcuI CTGAAG 3 cut(s) 34, 480, 501
AfaI GTAC 2 cut(s) 25, 525
AfiI CCNNNNNNNGG 1 cut(s) 239
AgsI TTSAA 2 cut(s) 218, 305
AjnI CCWGG 1 cut(s) 511
Alw21I GWGCWC 1 cut(s) 247
Alw26I GTCTC 2 cut(s) 272, 461
Alw44I GTGCAC 1 cut(s) 243
AlwI GGATC 5 cut(s) 287, 300, 404, 480, 493
ApaLI GTGCAC 1 cut(s) 243
ApeKI GCWGC 1 cut(s) 401
ApoI RAATTY 1 cut(s) 300
AspS9I GGNCC 1 cut(s) 154
AsuHPI GGTGA 1 cut(s) 287
AvaII GGWCC 1 cut(s) 154
BaeGI GKGCMC 1 cut(s) 247
BamHI GGATCC 2 cut(s) 292, 485
BanII GRGCYC 1 cut(s) 209
BauI CACGAG 1 cut(s) 405
Bbv12I GWGCWC 1 cut(s) 247
BbvI GCAGC 1 cut(s) 388
BccI CCATC 1 cut(s) 140
BciT130I CCWGG 1 cut(s) 513
BclI TGATCA 1 cut(s) 127
BcoDI GTCTC 2 cut(s) 272, 461
BisI GCNGC 1 cut(s) 402
BlsI GCNGC 1 cut(s) 403
Bme1390I CCNGG 1 cut(s) 513
Bme18I GGWCC 1 cut(s) 154
BmgT120I GGNCC 1 cut(s) 154
BmiI GGNNCC 4 cut(s) 99, 206, 294, 487
BmrFI CCNGG 1 cut(s) 513
BmsI GCATC 2 cut(s) 31, 485
BsaJI CCNNGG 1 cut(s) 157
BsaXI ACNNNNNCTCC 2 cut(s) 456, 486
Bsc4I CCNNNNNNNGG 1 cut(s) 239
Bse1I ACTGG 1 cut(s) 417
BseBI CCWGG 1 cut(s) 513
BseDI CCNNGG 1 cut(s) 157
BseGI GGATG 1 cut(s) 352
BseLI CCNNNNNNNGG 1 cut(s) 239
BseNI ACTGG 1 cut(s) 417
BseSI GKGCMC 1 cut(s) 247
BseXI GCAGC 1 cut(s) 388
BseYI CCCAGC 1 cut(s) 268
BsgI GTGCAG 1 cut(s) 420
BsiHKAI GWGCWC 1 cut(s) 247
BslI CCNNNNNNNGG 1 cut(s) 239
BsmAI GTCTC 2 cut(s) 272, 461
Bsp1286I GDGCHC 2 cut(s) 209, 247
Bsp143I GATC 5 cut(s) 127, 292, 364, 409, 485
Bsp19I CCATGG 1 cut(s) 157
BspLI GGNNCC 4 cut(s) 99, 206, 294, 487
BspPI GGATC 5 cut(s) 287, 300, 404, 480, 493
BspQI GCTCTTC 1 cut(s) 423
BsrI ACTGG 1 cut(s) 417
BssECI CCNNGG 1 cut(s) 157
BssMI GATC 5 cut(s) 127, 292, 364, 409, 485
BssSI CACGAG 1 cut(s) 405
BssT1I CCWWGG 1 cut(s) 157
Bst2BI CACGAG 1 cut(s) 405
Bst2UI CCWGG 1 cut(s) 513
Bst4CI ACNGT 3 cut(s) 283, 472, 528
Bst6I CTCTTC 2 cut(s) 63, 423
BstC8I GCNNGC 2 cut(s) 46, 435
BstDEI CTNAG 2 cut(s) 221, 567
BstDSI CCRYGG 1 cut(s) 157
BstF5I GGATG 1 cut(s) 352
BstKTI GATC 5 cut(s) 130, 295, 367, 412, 488
BstMAI GTCTC 2 cut(s) 272, 461
BstMBI GATC 5 cut(s) 127, 292, 364, 409, 485
BstNI CCWGG 1 cut(s) 513
BstNSI RCATGY 2 cut(s) 251, 546
BstSCI CCNGG 1 cut(s) 511
BstSLI GKGCMC 1 cut(s) 247
BstV1I GCAGC 1 cut(s) 388
BstX2I RGATCY 2 cut(s) 292, 485
BstYI RGATCY 2 cut(s) 292, 485
BtgI CCRYGG 1 cut(s) 157
BtsCI GGATG 1 cut(s) 352
Cac8I GCNNGC 2 cut(s) 46, 435
Cfr13I GGNCC 1 cut(s) 154
Csp6I GTAC 2 cut(s) 24, 524
CviAII CATG 6 cut(s) 116, 136, 158, 248, 265, 543
CviJI RGCY 4 cut(s) 207, 391, 433, 445
CviKI_1 RGCY 4 cut(s) 207, 391, 433, 445
CviQI GTAC 2 cut(s) 24, 524
DdeI CTNAG 2 cut(s) 221, 567
DpnI GATC 5 cut(s) 129, 294, 366, 411, 487
DpnII GATC 5 cut(s) 127, 292, 364, 409, 485
Eam1104I CTCTTC 2 cut(s) 63, 423
EarI CTCTTC 2 cut(s) 63, 423
Eco130I CCWWGG 1 cut(s) 157
Eco24I GRGCYC 1 cut(s) 209
Eco47I GGWCC 1 cut(s) 154
Eco57I CTGAAG 3 cut(s) 34, 480, 501
EcoRII CCWGG 1 cut(s) 511
EcoT14I CCWWGG 1 cut(s) 157
EcoT38I GRGCYC 1 cut(s) 209
ErhI CCWWGG 1 cut(s) 157
FaeI CATG 6 cut(s) 119, 139, 161, 251, 268, 546
FatI CATG 6 cut(s) 115, 135, 157, 247, 264, 542
FbaI TGATCA 1 cut(s) 127
Fnu4HI GCNGC 1 cut(s) 402
FokI GGATG 1 cut(s) 359
FriOI GRGCYC 1 cut(s) 209
Fsp4HI GCNGC 1 cut(s) 402
GluI GCNGC 1 cut(s) 402
GsaI CCCAGC 1 cut(s) 272
Hin1II CATG 6 cut(s) 119, 139, 161, 251, 268, 546
HinfI GANTC 4 cut(s) 55, 178, 320, 563
HphI GGTGA 1 cut(s) 287
Hpy166II GTNNAC 1 cut(s) 245
Hpy188I TCNGA 2 cut(s) 325, 481
Hpy188III TCNNGA 8 cut(s) 13, 52, 62, 202, 344, 368, 407, 498
Hpy8I GTNNAC 1 cut(s) 245
HpyAV CCTTC 3 cut(s) 111, 476, 504
HpyCH4III ACNGT 3 cut(s) 283, 472, 528
HpyCH4V TGCA 7 cut(s) 245, 251, 288, 335, 386, 437, 476
HpyF3I CTNAG 2 cut(s) 221, 567
Hsp92II CATG 6 cut(s) 119, 139, 161, 251, 268, 546
Ksp22I TGATCA 1 cut(s) 127
Kzo9I GATC 5 cut(s) 127, 292, 364, 409, 485
LguI GCTCTTC 1 cut(s) 423
LmnI GCTCC 1 cut(s) 204
Lsp1109I GCAGC 1 cut(s) 388
LweI GCATC 2 cut(s) 31, 485
MaeIII GTNAC 1 cut(s) 164
MalI GATC 5 cut(s) 129, 294, 366, 411, 487
MboI GATC 5 cut(s) 127, 292, 364, 409, 485
MboII GAAGA 3 cut(s) 50, 437, 440
MflI RGATCY 2 cut(s) 292, 485
MhlI GDGCHC 2 cut(s) 209, 247
MluCI AATT 1 cut(s) 300
MlyI GAGTC 2 cut(s) 49, 187
MmeI TCCRAC 1 cut(s) 437
MnlI CCTC 8 cut(s) 145, 155, 190, 266, 306, 371, 445, 550
MspR9I CCNGG 1 cut(s) 513
MvaI CCWGG 1 cut(s) 513
NcoI CCATGG 1 cut(s) 157
NdeII GATC 5 cut(s) 127, 292, 364, 409, 485
NlaIII CATG 6 cut(s) 119, 139, 161, 251, 268, 546
NlaIV GGNNCC 4 cut(s) 99, 206, 294, 487
NspI RCATGY 2 cut(s) 251, 546
PciSI GCTCTTC 1 cut(s) 423
PfeI GAWTC 2 cut(s) 320, 563
PkrI GCNGC 1 cut(s) 403
PleI GAGTC 2 cut(s) 49, 186
PpsI GAGTC 2 cut(s) 49, 186
Psp6I CCWGG 1 cut(s) 511
PspFI CCCAGC 1 cut(s) 268
PspGI CCWGG 1 cut(s) 511
PspN4I GGNNCC 4 cut(s) 99, 206, 294, 487
PspPI GGNCC 1 cut(s) 154
PsuI RGATCY 2 cut(s) 292, 485
RsaI GTAC 2 cut(s) 25, 525
RsaNI GTAC 2 cut(s) 24, 524
SapI GCTCTTC 1 cut(s) 423
SatI GCNGC 1 cut(s) 402
Sau3AI GATC 5 cut(s) 127, 292, 364, 409, 485
Sau96I GGNCC 1 cut(s) 154
SchI GAGTC 2 cut(s) 49, 187
ScrFI CCNGG 1 cut(s) 513
SduI GDGCHC 2 cut(s) 209, 247
SfaNI GCATC 2 cut(s) 31, 485
SinI GGWCC 1 cut(s) 154
Sse9I AATT 1 cut(s) 300
SspI AATATT 1 cut(s) 142
StyD4I CCNGG 1 cut(s) 511
StyI CCWWGG 1 cut(s) 157
TaaI ACNGT 3 cut(s) 283, 472, 528
TaqI TCGA 4 cut(s) 104, 363, 369, 507
TasI AATT 1 cut(s) 300
TatI WGTACW 1 cut(s) 23
TfiI GAWTC 2 cut(s) 320, 563
TseI GCWGC 1 cut(s) 401
TspDTI ATGAA 2 cut(s) 152, 186
VneI GTGCAC 1 cut(s) 243
VpaK11BI GGWCC 1 cut(s) 154
XapI RAATTY 1 cut(s) 300
XceI RCATGY 2 cut(s) 251, 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.